EWM21795.1, cds8880 (CDS) Nannochloropsis gaditana

BLAST of EWM21795.1 vs. NCBI_GenBank
Match: gi|585102131|gb|EWM21795.1| (bifunctional dihydrofolate reductase-thymidylate synthase [Nannochloropsis gaditana])

HSP 1 Score: 1093.95 bits (2828), Expect = 0.000e+0
Identity = 525/525 (100.00%), Postives = 525/525 (100.00%), Query Frame = 0

Query:    1 MPSTAAAAAMSTSAAAAATGKTEALRGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLNLPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525
            MPSTAAAAAMSTSAAAAATGKTEALRGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLNLPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV
Sbjct:    1 MPSTAAAAAMSTSAAAAATGKTEALRGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLNLPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525          

BLAST of EWM21795.1 vs. NCBI_GenBank
Match: gi|303275197|ref|XP_003056897.1| (bifunctional dihydrofolate reductase-thymidylate synthase [Micromonas pusilla CCMP1545] >gi|226461249|gb|EEH58542.1| bifunctional dihydrofolate reductase-thymidylate synthase [Micromonas pusilla CCMP1545])

HSP 1 Score: 610.527 bits (1573), Expect = 0.000e+0
Identity = 307/522 (58.81%), Postives = 365/522 (69.92%), Query Frame = 0

Query:   26 RGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLN--------------LPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFPVIPATEYCL-VSSTPVLSQNALRFRFQRYERLD------RGP-SADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525
            R F +VVAAT    GIG  G LPW LP+DM +FK +TA  + P K NAVIMGR+TW+SIP KFRPL  RLNVVLS++  + E                 LP  V +  SL+AALA L  G  A  VEK+FV+GGA VY EA+ S AC+A+  T +   +  TP    CD H P +   ++ L  S+ PV  +   + +F  Y   D      R P S  LP G   N V       EE QYL L+++IIE G  +GDRT TGT+SKFG  +RF LR  +FPLLTTKRVFWRGVAEELLWF+ G T+A  L++K I IWDGN SREYLD +GLSHREE DLGPVYGFQWRHFGAEY+DMHA+Y GKG+DQLA  I  IK+NP DRRI+++AWNPA L +MALPPCHMFCQFYVA+GELSCQMYQRS DMGLGVPFNIASY+LLT ++AQVCGLK G+F+H +GD HVY NHV+ LK+QL   PR FP L+INPAK DIDSF F DFTIEGY PH  ++MKMAV
Sbjct:   10 RPFQVVVAATKGDMGIGLDGALPWRLPKDMAYFKSVTATVAEPGKRNAVIMGRKTWESIPAKFRPLPGRLNVVLSKSGALVESTGSENAAPANGTPSNVLPPSVLLQPSLEAALATLGAGEHAENVEKIFVVGGAQVYAEAMASPACQAVHITEVTPPKD-TPDAFKCDTHMPPVDMEKFKLYASAPPVNEKEGAKIQFLSYVAKDAATGKFRIPGSVSLPPGAVANGV-----RHEELQYLDLIRDIIESGNVKGDRTGTGTISKFGTSMRFDLR-RSFPLLTTKRVFWRGVAEELLWFVGGRTNAKELQDKDIHIWDGNGSREYLDSVGLSHREEQDLGPVYGFQWRHFGAEYTDMHANYDGKGVDQLAEVIDKIKNNPNDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCGLKPGDFVHVMGDTHVYSNHVEPLKKQLLCEPRPFPTLKINPAKTDIDSFEFSDFTIEGYDPHPKIEMKMAV 524          

BLAST of EWM21795.1 vs. NCBI_GenBank
Match: gi|308799723|ref|XP_003074642.1| (bifunctional dihydrofolate reductase-thymidylate synthase 2 / DHFR-TS (ISS) [Ostreococcus tauri])

HSP 1 Score: 605.52 bits (1560), Expect = 0.000e+0
Identity = 302/528 (57.20%), Postives = 375/528 (71.02%), Query Frame = 0

Query:    7 AAAMSTSAAAAATGKTEALRGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLN---------LPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525
            A+A +   A  + G  E +R F +VVAAT    GIGK   LPW L +DM HFK+LT+ T   + +NAV+MGR+TW+SIP KFRPL  RLNVVLSR+ G+  + N         LP+GV V  ++  AL+ ++  +    VEK FVIGGA +Y EA++SE CEA+  T +E +        +CD   P I A ++ L   +  +++N  R++F  Y   D       P+  G    +G     EE QYL +++EII+ G  +GDRT TGT+S FG Q+RF LR  +FPLLTTKRVFWRGVAEELLWF++G T+AN L EKKI IWDGN SREYLD +GL+ RE GDLGPVYGFQWRHFGAEY++MHADYTGKG+DQLA  I  IK+NP DRRI+++AWNPA L +MALPPCHMFCQFYVA+GELSCQMYQRS DMGLGVPFNIASY+LLT ++AQVC LK G+F+H  GD HVY NHV+ LK+QLT  PR FP L+INP KKDIDSFTF+DF I GY PH  ++MKMAV
Sbjct:  238 ASARAIGEAKRSRGGME-MRAFQVVVAATVDG-GIGKDNGLPWKLTKDMGHFKKLTSETRDANAVNAVVMGRKTWESIPAKFRPLPGRLNVVLSRS-GVLAEANDENNNGAETLPEGVLVRKTIDDALSAIS--SSEKRVEKTFVIGGAQIYEEALKSERCEAVHLTEVEGD-------FECDVFIPKIDADKFKLYGQSKPITENGARYQFLTYVTKDSESGKFRPIA-GELLPAGCDVKHEEHQYLDMIREIIDQGAVKGDRTGTGTISTFGNQMRFDLR-RSFPLLTTKRVFWRGVAEELLWFVAGETNANKLAEKKINIWDGNGSREYLDSIGLTEREVGDLGPVYGFQWRHFGAEYTNMHADYTGKGVDQLAEVINKIKNNPTDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCDLKPGDFVHVCGDTHVYSNHVEPLKKQLTCEPRPFPILKINPEKKDIDSFTFDDFEIIGYDPHPKIEMKMAV 751          

BLAST of EWM21795.1 vs. NCBI_GenBank
Match: gi|1199292847|gb|OUS47933.1| (thymidylate synthase/dCMP hydroxymethylase domain-containing protein [Ostreococcus tauri])

HSP 1 Score: 603.594 bits (1555), Expect = 0.000e+0
Identity = 298/510 (58.43%), Postives = 368/510 (72.16%), Query Frame = 0

Query:   25 LRGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLN---------LPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525
            +R F +VVAAT    GIGK   LPW L +DM HFK+LT+ T   + +NAV+MGR+TW+SIP KFRPL  RLNVVLSR+ G+  + N         LP+GV V  ++  AL+ ++  +    VEK FVIGGA +Y EA++SE CEA+  T +E +        +CDA  P I A ++ L   +  +++N  R++F  Y   D       P+  G    +G     EE QYL +++EII+ G  +GDRT TGT+S FG Q+RF LR  +FPLLTTKRVFWRGVAEELLWF++G T+AN L EKKI IWDGN SREYLD +GL+ RE GDLGPVYGFQWRHFGAEY++MHADYTGKG+DQLA  I  IK+NP DRRI+++AWNPA L +MALPPCHMFCQFYVA+GELSCQMYQRS DMGLGVPFNIASY+LLT ++AQVC LK G+F+H  GD HVY NHV+ LK+QLT  PR FP L+INP KKDIDSFTF+DF I GY PH  ++MKMAV
Sbjct:    1 MRAFQVVVAATVDG-GIGKDNGLPWKLTKDMGHFKKLTSETRDANAVNAVVMGRKTWESIPAKFRPLPGRLNVVLSRS-GVLAEANDENNNGAETLPEGVLVRKTIDDALSAIS--SSEKRVEKTFVIGGAQIYEEALKSERCEAVHLTEVEGD-------FECDAFIPKIDADKFKLYGQSKPITENGARYQFLTYVTKDSESGKFRPIA-GELLPAGCDVKHEEHQYLDMIREIIDQGAVKGDRTGTGTISTFGNQMRFDLR-RSFPLLTTKRVFWRGVAEELLWFVAGETNANKLAEKKINIWDGNGSREYLDSIGLTEREVGDLGPVYGFQWRHFGAEYTNMHADYTGKGVDQLAEVINKIKNNPTDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCDLKPGDFVHVCGDTHVYSNHVEPLKKQLTCEPRPFPILKINPEKKDIDSFTFDDFEIIGYDPHPKIEMKMAV 497          

BLAST of EWM21795.1 vs. NCBI_GenBank
Match: gi|693501883|emb|CEF96887.1| (Thymidylate synthase [Ostreococcus tauri])

HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0
Identity = 297/510 (58.24%), Postives = 367/510 (71.96%), Query Frame = 0

Query:   25 LRGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLN---------LPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525
            +R F +VVAAT    GIGK   LPW L +DM HFK+LT+ T   + +NAV+MGR+TW+SIP KFRPL  RLNVVLSR+ G+  + N         LP+GV V  ++  AL+ ++  +    VEK FVIGGA +Y EA++SE CEA+  T +E +        +CD   P I A ++ L   +  +++N  R++F  Y   D       P+  G    +G     EE QYL +++EII+ G  +GDRT TGT+S FG Q+RF LR  +FPLLTTKRVFWRGVAEELLWF++G T+AN L EKKI IWDGN SREYLD +GL+ RE GDLGPVYGFQWRHFGAEY++MHADYTGKG+DQLA  I  IK+NP DRRI+++AWNPA L +MALPPCHMFCQFYVA+GELSCQMYQRS DMGLGVPFNIASY+LLT ++AQVC LK G+F+H  GD HVY NHV+ LK+QLT  PR FP L+INP KKDIDSFTF+DF I GY PH  ++MKMAV
Sbjct:    3 MRAFQVVVAATVDG-GIGKDNGLPWKLTKDMGHFKKLTSETRDANAVNAVVMGRKTWESIPAKFRPLPGRLNVVLSRS-GVLAEANDENNNGAETLPEGVLVRKTIDDALSAIS--SSEKRVEKTFVIGGAQIYEEALKSERCEAVHLTEVEGD-------FECDVFIPKIDADKFKLYGQSKPITENGARYQFLTYVTKDSESGKFRPIA-GELLPAGCDVKHEEHQYLDMIREIIDQGAVKGDRTGTGTISTFGNQMRFDLR-RSFPLLTTKRVFWRGVAEELLWFVAGETNANKLAEKKINIWDGNGSREYLDSIGLTEREVGDLGPVYGFQWRHFGAEYTNMHADYTGKGVDQLAEVINKIKNNPTDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCDLKPGDFVHVCGDTHVYSNHVEPLKKQLTCEPRPFPILKINPEKKDIDSFTFDDFEIIGYDPHPKIEMKMAV 499          

BLAST of EWM21795.1 vs. NCBI_GenBank
Match: gi|676394827|ref|XP_009040691.1| (hypothetical protein AURANDRAFT_38942 [Aureococcus anophagefferens] >gi|323448689|gb|EGB04584.1| hypothetical protein AURANDRAFT_38942 [Aureococcus anophagefferens])

HSP 1 Score: 602.053 bits (1551), Expect = 0.000e+0
Identity = 301/508 (59.25%), Postives = 358/508 (70.47%), Query Frame = 0

Query:   22 TEALRGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPD-KINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLNLPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAG---NQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525
            T  L   T +VAA A ++GIGK G LPW L  DM++FK++T  ++ P+ K NA +MGR+TW SIP KFRPL  R NVVLSRNP  RE+L LP  V V  SL AA+  LA   D   + +VFVIGG  VY  A+    C  +  TSI++  GA   FADCDA FP      +   +S   L +N + +RF+   R  + P      G      +       N EE QYL L+ EI++ G RRGDRT TGT+S FGA++ FSLR++ FPLLTTKRVFWRGVAEEL+WF+SGST+   L +K I IWDGN SRE+LD+ GL HREE DLGPVYGFQWRHFGA+Y DMH DY G+G+DQLA  + TIK +P  RRI++SAWNPADL +MALPPCHMFCQFYVA+GELSCQMYQRSADMGLGVPFNIASYALLTR+VAQ  GLK G+F+H IGDAHVY NHVDALK QL R PR FP L INPAK  ID F F DFT+EGY P+  + MKMAV
Sbjct:   11 TNDLPQLTCIVAAVAGSYGIGKDGTLPWKLAGDMKYFKKVT--SAAPEGKTNACVMGRKTWLSIPPKFRPLGGRKNVVLSRNPNAREELGLPPDVFVCPSLDAAVDALAGEAD---LGEVFVIGGGQVYASALADARCTKVLLTSIDDAAGA---FADCDAVFPDPAKAGFASAASGEALVENGVAYRFETLTRPAKKPPLRESNGPPAPPPANEKPAAPNHEEKQYLDLIAEILDRGVRRGDRTGTGTISIFGARMSFSLRNDRFPLLTTKRVFWRGVAEELVWFVSGSTNGKLLADKGIHIWDGNGSREFLDRRGLGHREEMDLGPVYGFQWRHFGAKYVDMHKDYAGEGVDQLAACVETIKQDPNSRRIVLSAWNPADLDEMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRMVAQATGLKPGDFVHVIGDAHVYSNHVDALKVQLQREPRPFPTLTINPAKTGIDDFEFADFTVEGYKPYPKIDMKMAV 510          

BLAST of EWM21795.1 vs. NCBI_GenBank
Match: gi|145346608|ref|XP_001417778.1| (predicted protein [Ostreococcus lucimarinus CCE9901] >gi|144578006|gb|ABO96071.1| predicted protein [Ostreococcus lucimarinus CCE9901])

HSP 1 Score: 598.586 bits (1542), Expect = 0.000e+0
Identity = 295/506 (58.30%), Postives = 365/506 (72.13%), Query Frame = 0

Query:   28 FTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLN--------LPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525
            F +VVAAT +  GIG+   LPW L  DM +FK++T+ T   D +NAV+MGR+TW+SIP KFRPL  RLN+VLSR+ G+ E  +        LP+GV V  S+  AL+ ++  +    +EK FVIGGA +Y EA++SE CEA+  T +E E        +CDA  P I AT++ L   +  + +   RF+F  Y   D       P  D     +G +   EE QYL +++EII+ G  +GDRT TGT+S FG Q+RF LR  +FPLLTTKRVFWRGVAEELLWF++G T+AN L EKKI IWDGN SREYLD +GL+ RE GDLGPVYGFQWRHFGAEY++MHADYTGKG+DQLA  I  IK+NP DRRI+++AWNPA L +MALPPCHMFCQFYVA+GELSCQMYQRS DMGLGVPFNIASY+LLT ++AQVCGLK G+F+H  GD HVY NHV+ L++QL   PR FP L+INP KKDIDSFTF+DF I GY PH  ++MKMAV
Sbjct:    6 FQVVVAAT-REGGIGRENALPWRLAGDMGYFKKITSETRDGDAMNAVVMGRKTWESIPGKFRPLPGRLNIVLSRSGGLAEANDENNNGAETLPEGVLVRKSIDDALSAIS--SSEKRIEKTFVIGGAQIYEEALQSEKCEAVHLTEVEGE-------FECDAFIPKIDATKFKLYGQSKPMIEKGTRFQFLTYVTADAESGKFRPKAD-EVLPAGCSIKHEEYQYLEMIREIIDQGAVKGDRTGTGTISTFGNQMRFDLR-RSFPLLTTKRVFWRGVAEELLWFVAGETNANKLAEKKINIWDGNGSREYLDSIGLTEREVGDLGPVYGFQWRHFGAEYTNMHADYTGKGVDQLAEVIHKIKNNPNDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCGLKPGDFVHCCGDTHVYSNHVEPLEKQLACEPRPFPILKINPEKKDIDSFTFDDFEIVGYDPHPKIEMKMAV 499          

BLAST of EWM21795.1 vs. NCBI_GenBank
Match: gi|698790846|ref|XP_009828455.1| (thymidylate synthase [Aphanomyces astaci] >gi|574116234|gb|ETV81718.1| thymidylate synthase [Aphanomyces astaci])

HSP 1 Score: 596.275 bits (1536), Expect = 0.000e+0
Identity = 296/504 (58.73%), Postives = 356/504 (70.63%), Query Frame = 0

Query:   25 LRGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQL--NLPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFP-VIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525
            +   ++VVA   KT+GIG    LPW LP DM+ F+ +T  T+   K NAVIMGR TW+SIP KFRPL  RLNVVL+RN  +  +L  + PQ V  A+SL  AL+ L     ++ +E VF IGGA+VY +A+   AC     T ++ +        DCDA FP  +    +    +     +N + F F  YER                         EE QYL L+Q I++ G ++GDRT TGTLS FGAQ+RFSLRD+ FPLLTTKRVFW+GVAEELLWFISG+T+A  L++K I IWDGN SREYLD +GL HREEGDLGPVYGFQWRHFGA+Y DMH DYTG+G DQLA  I  IK  P DRRII+SAWNPADLG MALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRL+AQVCGL+AG+FIH  GDAHVYLNHV  L+EQL R+PR FP L++N AK +ID FTF+DFT++GY PH+++KM M+V
Sbjct:    1 MHEISVVVAVARKTWGIGINNALPWKLPSDMKRFREITTGTTDATKQNAVIMGRNTWESIPAKFRPLPGRLNVVLTRNAQLAAELEASSPQ-VLAASSLNDALSKLP----SATIEHVFAIGGASVYSDALRHPACHRAYVTLVDGD-------FDCDAFFPSTLKQLGFVETEALGTQRENDIDFHFATYER-----------------------THEELQYLALIQRILDDGIQKGDRTGTGTLSLFGAQMRFSLRDDVFPLLTTKRVFWKGVAEELLWFISGNTNAKTLQDKGIKIWDGNGSREYLDSIGLVHREEGDLGPVYGFQWRHFGAKYIDMHTDYTGQGHDQLADVIYKIKHTPNDRRIILSAWNPADLGIMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLLAQVCGLQAGDFIHVFGDAHVYLNHVAPLQEQLKRSPRPFPTLKVNAAKTEIDDFTFDDFTLDGYHPHKTIKMDMSV 469          

BLAST of EWM21795.1 vs. NCBI_GenBank
Match: gi|813151154|ref|XP_012201157.1| (bifunctional dihydrofolate reductase-thymidylate synthase 2 [Saprolegnia parasitica CBS 223.65] >gi|641534078|gb|KDO28037.1| bifunctional dihydrofolate reductase-thymidylate synthase 2 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 595.89 bits (1535), Expect = 0.000e+0
Identity = 291/499 (58.32%), Postives = 357/499 (71.54%), Query Frame = 0

Query:   28 FTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLNLPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFP-VIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525
             +++VA    T GIG  GQLPW L  D++ F+ LT  T+   K NAV+MGR+TW+S+P K RPL  R+NVVLSRNP +R  L+   GV  A +L  AL  L      + VE VF+IGG ++Y EA +   C     T ++ E        +CDA FP  + +  +   S +PV  +  + F+   Y +LDR                      EE QYL L+Q+I++ G  +GDRT TGT+S FGAQ+RFSLR + FPLLTTKRVFW+GVAEELLWFISG T+A  L++K I IWDGN SRE+LDKLGL+HRE GDLGPVYGFQWRHFGA+Y DM+ DYTG+G+DQLA  I  IK +P DRRI++SAWNP+DLG MALPPCHMFCQFYVA+GELSCQMYQRSADMGLGVPFNIASYALLTR++AQVCGLKAG+FIH IGDAHVY NHV+ L+EQL RTP  FP L+INPAK DID FTF+DFTIEGY P +++KM+MA+
Sbjct:    4 LSMIVAVARATNGIGIKGQLPWRLAGDLKRFRELTTSTADATKQNAVVMGRKTWESLPLKHRPLPGRVNVVLSRNPAVRAALSAQTGVLAAGNLDEALGAL------TGVEHVFIIGGQSLYEEAAQHPRCTRAYLTLVDGEY-------ECDAFFPKSLASCGFAETSVSPVQIEKDISFQ---YIQLDR--------------------KHEELQYLELIQKILDEGVHKGDRTGTGTISLFGAQMRFSLRHDVFPLLTTKRVFWKGVAEELLWFISGDTNAKTLQDKGIKIWDGNGSREFLDKLGLTHREAGDLGPVYGFQWRHFGAKYVDMYTDYTGQGVDQLADVIHKIKHSPNDRRILLSAWNPSDLGLMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRMIAQVCGLKAGDFIHVIGDAHVYSNHVEPLQEQLKRTPSPFPMLKINPAKMDIDKFTFDDFTIEGYTPQKTIKMEMAI 466          

BLAST of EWM21795.1 vs. NCBI_GenBank
Match: gi|552833481|ref|XP_005848544.1| (hypothetical protein CHLNCDRAFT_30873 [Chlorella variabilis] >gi|307108201|gb|EFN56442.1| hypothetical protein CHLNCDRAFT_30873 [Chlorella variabilis])

HSP 1 Score: 593.964 bits (1530), Expect = 0.000e+0
Identity = 297/517 (57.45%), Postives = 364/517 (70.41%), Query Frame = 0

Query:   23 EALRGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRN----------PG----IREQLNLPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEERGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLDRGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525
            +A R   IVVAAT K +GIGK G LPW+LP DM++F+ LT+ T+ P K N VIMGR+TW+SIP KFRPL  R+NVVL+R           PG       + +  +GV +++SL +AL +L+     S VE VFVIGG  VY+E +ES    AI  T +E E       A+CD   P +  + + L S++   S    R+ F  Y R  +     LP           A   EE QYL ++++++ +G  RGDRT TGT SKFGA +RF+LR  +FPLLTTKRVFWRGVAEELLWF+SG T+A  L +K I IWDGN SREYLD +GLSHREE DLGPVYGFQWRHFGAEY+DMHADY GKG+DQLA  I  IK NP DRR+++SAWNPA L  MALPPCHMFCQFYVADGELSCQMYQRS D+GLGVPFNIASY+LLT +VAQVCGL+ G+F+H +GDAHVY NHV  L++QL   PR FP+L INPAK DIDSF F+DF +EGY PH+++KM+MAV
Sbjct:    5 QARRTIQIVVAAT-KQWGIGKGGSLPWSLPGDMKYFRELTSRTADPAKQNVVIMGRKTWESIPAKFRPLAGRINVVLTRGAVAGDENASAPGNATAALAEASKAEGVHISSSLDSALEMLSGPEFDSRVESVFVIGGGQVYKECMESPLLSAIHLTLVEGEAAEA---ANCDTFMPPVDESRFRLWSASAPRSVGGTRYSFLCYTRAGQEGLPALP--------PTVASRHEEHQYLEMIEDVMANGVFRGDRTGTGTYSKFGATMRFNLR-HSFPLLTTKRVFWRGVAEELLWFVSGCTNARVLMDKGIHIWDGNGSREYLDSIGLSHREEMDLGPVYGFQWRHFGAEYTDMHADYGGKGVDQLAELIHKIKTNPNDRRLVLSAWNPAALTDMALPPCHMFCQFYVADGELSCQMYQRSCDLGLGVPFNIASYSLLTCMVAQVCGLRPGDFVHVLGDAHVYANHVGPLRDQLKNAPRHFPRLLINPAKTDIDSFVFDDFQLEGYQPHKAIKMQMAV 508