EWM29848.1, cds872 (CDS) Nannochloropsis gaditana

BLAST of EWM29848.1 vs. NCBI_GenBank
Match: gi|585112429|gb|EWM29848.1| (beta- gba2 type family protein [Nannochloropsis gaditana])

HSP 1 Score: 2147.47 bits (5563), Expect = 0.000e+0
Identity = 1040/1040 (100.00%), Postives = 1040/1040 (100.00%), Query Frame = 0

Query:    1 MPGLKESLSLLPLAWRMGQYVSGERRENRDPIFDLNGLLLQPPIPGPYAGVPLGGLGGGCIGRGFRGEFRRWNLNPGKYRHKVVAANQFSVRVLRKGQAPVAQVLAVGDGRETDGLRAWRWGMDPRVGTYHALFPRAWTVYEEAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRFSSGGAGSKEASEVWRQFLEDGSLREIGRTEEQHGDQDHLETLATRTRGSDRIVETSECALERPSEGRGRGGRVGEQGDGHTDGRHHRYHHHHHRLHHHAGNGKHRRPQHSKEAGPRRPHHRPSKAKESIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWTESRAGRAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDARRRAGGASGVENNRTFQSCHGEQRMVGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGWKRFGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTRMPATTVSARPADLVGRTDDELEQCDKRRDDDTINGAEQHFSHTNGVQTRNNADQVCNK 1040
            MPGLKESLSLLPLAWRMGQYVSGERRENRDPIFDLNGLLLQPPIPGPYAGVPLGGLGGGCIGRGFRGEFRRWNLNPGKYRHKVVAANQFSVRVLRKGQAPVAQVLAVGDGRETDGLRAWRWGMDPRVGTYHALFPRAWTVYEEAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRFSSGGAGSKEASEVWRQFLEDGSLREIGRTEEQHGDQDHLETLATRTRGSDRIVETSECALERPSEGRGRGGRVGEQGDGHTDGRHHRYHHHHHRLHHHAGNGKHRRPQHSKEAGPRRPHHRPSKAKESIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWTESRAGRAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDARRRAGGASGVENNRTFQSCHGEQRMVGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGWKRFGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTRMPATTVSARPADLVGRTDDELEQCDKRRDDDTINGAEQHFSHTNGVQTRNNADQVCNK
Sbjct:    1 MPGLKESLSLLPLAWRMGQYVSGERRENRDPIFDLNGLLLQPPIPGPYAGVPLGGLGGGCIGRGFRGEFRRWNLNPGKYRHKVVAANQFSVRVLRKGQAPVAQVLAVGDGRETDGLRAWRWGMDPRVGTYHALFPRAWTVYEEAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRFSSGGAGSKEASEVWRQFLEDGSLREIGRTEEQHGDQDHLETLATRTRGSDRIVETSECALERPSEGRGRGGRVGEQGDGHTDGRHHRYHHHHHRLHHHAGNGKHRRPQHSKEAGPRRPHHRPSKAKESIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWTESRAGRAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDARRRAGGASGVENNRTFQSCHGEQRMVGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGWKRFGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTRMPATTVSARPADLVGRTDDELEQCDKRRDDDTINGAEQHFSHTNGVQTRNNADQVCNK 1040          

BLAST of EWM29848.1 vs. NCBI_GenBank
Match: gi|168014916|ref|XP_001759997.1| (predicted protein [Physcomitrella patens] >gi|162688747|gb|EDQ75122.1| predicted protein [Physcomitrella patens])

HSP 1 Score: 517.694 bits (1332), Expect = 4.524e-164
Identity = 290/616 (47.08%), Postives = 363/616 (58.93%), Query Frame = 0

Query:  399 PSKAKESIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWTESRAGRAHESD---GEEEGGQGWETGRNRRPDQEGEVWRGNREQDARRRAGGASGVENNRTFQS----CHGEQRMVGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGWKRFGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTRMPATTVSARPADLVGRTDDELEQCD 1007
            PSK    I AA+   V + P+    + F LAWD P V+F  G +  RRYT F+  +G   A  IA +AL  Y LWE+EIE WQRP+LED  LP +Y+  LFNELY+L  GGTVWT S A  +       G+   G        RR      + R  ++ D      G++    N    S       E+  VGQFLY+EG EY+M+NTYDVHFYASFAL  L+P LEL++QRD AA  +  D    + +A G+   +KV G+VPHDLG     PW +VNAYN+ D +RWKDL  K VLQ YRD +AT    FA+AVWP +   M A L  FDRD DGLIEN GFPDQTYD WTV G SAY GGLW+AAL A  A+   +   N          ++Q+   QAREAY   LWNG+YF YD+  S +  SI ADQMAGQWYA A GL P+   A+ARS+L  +F +NV +  +G  GAVNGM P+G+VD+TCMQS+EVWTG TY++AAAM+ E  L                          AF  A G++ AGW   GYWF TPEAW  +G+FRSL YMRPL+IWAMQWA+   P  T++  P   +   D    +CD
Sbjct:  354 PSKPGSVIGAAIAAHVVIPPNEKRTIDFVLAWDSPTVKFLKGRSYLRRYTTFYDADGN-AAPRIAHNALCGYRLWEEEIEKWQRPILEDEKLPEWYRVTLFNELYYLVAGGTVWTASSANTSAPGSPLHGDLSLGGKVVPFSTRRDIDLAALRRDCKDGDVVAALEGSNKSSFNSRLPSGLALLQAEEENVGQFLYLEGIEYIMWNTYDVHFYASFALLSLFPKLELAIQRDFAAGCLAHDPRRTKFMAGGKKGIKKVFGSVPHDLG--QHDPWAEVNAYNIHDTSRWKDLNAKFVLQVYRDVVATGDKVFAKAVWPAVYAAM-AYLDQFDRDRDGLIENDGFPDQTYDTWTVHGVSAYCGGLWIAALQAAEAMAVLLEEKNAAR-------YFQSKFIQAREAYLKKLWNGSYFSYDSGNSSNSKSIQADQMAGQWYAWASGLPPLFDDAKARSALQKIFDFNVMKVKNGHLGAVNGMHPNGKVDETCMQSREVWTGVTYALAAAMIHEGML------------------------DQAFRAAEGVFIAGWSDLGYWFQTPEAWTMDGYFRSLAYMRPLAIWAMQWAID--PPETIANAPR--IPTVDRLPSECD 930          

HSP 2 Score: 174.096 bits (440), Expect = 9.589e-41
Identity = 120/314 (38.22%), Postives = 161/314 (51.27%), Query Frame = 0

Query:    4 LKESLSLLPLAWRMGQYVSGERRENRDPIFDLNGLLLQPPIPGPYAGVPLGGLGGGCIGRGFRGEFRRWNLNPGKYRHKVVAANQFSVRV-LRKGQAPVAQVLAVGDGRETDGLR-------AWRWGMDPRVGTYHALFPRAWTVYE-EAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRFSSGGAG-SKEASEVWRQFLEDGSLREIGRTE 307
              E   +L L  RM  YV  E+ E R P  D      +   P    GVP GG+GGG IGRGFRGEFRRW + PG      V ANQFSV V L  G+  V+ VL  G  RE  GL+       +W W +D +  TYHALFPRAWT+YE E  P ++++CRQ+SP +PH+Y++SS P CVF +   N G   ADV+++F++ N +G+++   GGH N  F+     +S +GG+                               LTFA+AA       +S C  F   G G S  A ++W      G ++E GR E
Sbjct:   73 FMEGFKMLRLGMRMWTYVQSEKSEGRLPPIDP---FRRETKPSACHGVPCGGMGGGSIGRGFRGEFRRWQIVPGVCDEAPVLANQFSVHVSLSGGEKAVSSVLYPGRPRELKGLKNDENGISSWDWKLDGQNTTYHALFPRAWTIYEGEPDPALKISCRQISPFIPHNYKESSYPACVFSYVAVNTGKEVADVNLLFTWANSIGSTTGSTGGHINETFT-----TSVKGGQ------------------------------PLTFAIAAKQSPDVSLSACPCFQVSGEGQSITAKDLW------GRMKEHGRLE 342          

BLAST of EWM29848.1 vs. NCBI_GenBank
Match: gi|1173145249|ref|XP_020519085.1| (non-lysosomal glucosylceramidase isoform X2 [Amborella trichopoda])

HSP 1 Score: 509.22 bits (1310), Expect = 1.589e-162
Identity = 287/619 (46.37%), Postives = 365/619 (58.97%), Query Frame = 0

Query:  399 PSKAKESIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWT------------ESRAGRAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDA-----RRRAGGASGVENNRTFQSCHG------EQRMVGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGWKR--FGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTRMPATTVSARP 992
            PS+ + SI AAV  +V++ PH V  ++F+LAW    V+F SG    RRYT F+G +G   A L+    L +   WE +IEAWQ+P+++D +LP +Y+  LFNELY+L  GGT+WT            E R     +S+ E     G                   RE D       R       ++N  T  S  G      ++  +GQFLY EG EY M+NTYDVHFYASFAL ML+P LELS+QRD AAAVM  D    + L  G+  PRKV GAVPHDLG     PW +VNAYN+ DVNRWKDL PK VLQ YRD + T    FA +VWP +   M A +  FD+D DG+IEN GFPDQTYD+W+V G SAYTGGLWVAAL A +A+   V     G +A A   ++      A+  Y G LWNG+YF+YDNS   +  SI ADQ+AGQWYARACGL P+    +A+S+L  V+++NV +  DG  GAVNGM P+G VD + MQS+E+WTG TY+VAAAM+QE                         +  +AF+TA G+YEA W     GY F TPEAWN  G FRSL YMRPL+IWA+QWAL+       + +P
Sbjct:  223 PSEPESSIGAAVVASVTVPPHTVRNVTFSLAWACSKVKFPSGKIYHRRYTTFYGTQGDAAAKLVHDAILEHGS-WESQIEAWQKPIIQDKTLPEWYRITLFNELYYLNAGGTIWTDGLPPIESQVCVEDRKFSLDKSNFENTTSLG-------------------RENDTAIGILDRMTSILEEMQNPSTANSAFGPTLLLKDEENIGQFLYYEGIEYHMWNTYDVHFYASFALIMLFPKLELSIQRDFAAAVMMHDPERMKTLQDGKWVPRKVLGAVPHDLGLND--PWFEVNAYNIHDVNRWKDLNPKFVLQVYRDMVFTGDKSFAHSVWPSVYMAM-AYMDQFDKDRDGMIENEGFPDQTYDVWSVTGVSAYTGGLWVAALQAASAMAHEV-----GDNASA--DYFWHKFENAKRVY-GTLWNGSYFNYDNSDGAYSSSIQADQLAGQWYARACGLVPIVDDEKAQSALEKVYQFNVLKLGDGKRGAVNGMLPNGTVDMSTMQSREIWTGVTYAVAAAMIQEG------------------------MEDSAFKTAEGIYEASWSPEGLGYSFQTPEAWNTNGEFRSLSYMRPLAIWAIQWALSPPVLNNKTQKP 786          

HSP 2 Score: 122.479 bits (306), Expect = 1.030e-24
Identity = 69/191 (36.13%), Postives = 105/191 (54.97%), Query Frame = 0

Query:  115 GLRAWRWGMDPRVGTYHALFPRAWTVYE-EAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRFS-SGGAGSKEASEVWRQFLEDGSLREI 303
            G+ +W W ++    TYHAL+PRAWTVYE E  P +++ CRQ+SP++P++Y++SSLPV VF +T+ N G  AADVS++F+F N +G  S+ +G H N +  +                        V GV L H+    + +  +TFA+AA   +   VS C  F  SG +    A ++W +  E GS   +
Sbjct:   48 GIGSWDWNLNGEHSTYHALYPRAWTVYEDEPDPDLKIVCRQISPIIPNNYRESSLPVTVFTFTLINSGKDAADVSLLFTFANSVGGQSEFSGNHLNSQLRMMDG---------------------VRGVTLCHKVVNGQPE--VTFAIAAQETNDVHVSECPSFVISGNSQGFSARDMWHEVKEHGSFDRL 215          

BLAST of EWM29848.1 vs. NCBI_GenBank
Match: gi|586670556|ref|XP_006836825.1| (non-lysosomal glucosylceramidase isoform X1 [Amborella trichopoda] >gi|769819498|ref|XP_011620970.1| non-lysosomal glucosylceramidase isoform X1 [Amborella trichopoda] >gi|1173145236|ref|XP_020519079.1| non-lysosomal glucosylceramidase isoform X1 [Amborella trichopoda] >gi|1173145238|ref|XP_020519080.1| non-lysosomal glucosylceramidase isoform X1 [Amborella trichopoda] >gi|1173145241|ref|XP_020519081.1| non-lysosomal glucosylceramidase isoform X1 [Amborella trichopoda] >gi|1173145243|ref|XP_020519082.1| non-lysosomal glucosylceramidase isoform X1 [Amborella trichopoda] >gi|1173145245|ref|XP_020519083.1| non-lysosomal glucosylceramidase isoform X1 [Amborella trichopoda] >gi|1173145247|ref|XP_020519084.1| non-lysosomal glucosylceramidase isoform X1 [Amborella trichopoda] >gi|548839389|gb|ERM99678.1| hypothetical protein AMTR_s00099p00047790 [Amborella trichopoda])

HSP 1 Score: 508.834 bits (1309), Expect = 7.289e-161
Identity = 287/619 (46.37%), Postives = 365/619 (58.97%), Query Frame = 0

Query:  399 PSKAKESIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWT------------ESRAGRAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDA-----RRRAGGASGVENNRTFQSCHG------EQRMVGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGWKR--FGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTRMPATTVSARP 992
            PS+ + SI AAV  +V++ PH V  ++F+LAW    V+F SG    RRYT F+G +G   A L+    L +   WE +IEAWQ+P+++D +LP +Y+  LFNELY+L  GGT+WT            E R     +S+ E     G                   RE D       R       ++N  T  S  G      ++  +GQFLY EG EY M+NTYDVHFYASFAL ML+P LELS+QRD AAAVM  D    + L  G+  PRKV GAVPHDLG     PW +VNAYN+ DVNRWKDL PK VLQ YRD + T    FA +VWP +   M A +  FD+D DG+IEN GFPDQTYD+W+V G SAYTGGLWVAAL A +A+   V     G +A A   ++      A+  Y G LWNG+YF+YDNS   +  SI ADQ+AGQWYARACGL P+    +A+S+L  V+++NV +  DG  GAVNGM P+G VD + MQS+E+WTG TY+VAAAM+QE                         +  +AF+TA G+YEA W     GY F TPEAWN  G FRSL YMRPL+IWA+QWAL+       + +P
Sbjct:  339 PSEPESSIGAAVVASVTVPPHTVRNVTFSLAWACSKVKFPSGKIYHRRYTTFYGTQGDAAAKLVHDAILEHGS-WESQIEAWQKPIIQDKTLPEWYRITLFNELYYLNAGGTIWTDGLPPIESQVCVEDRKFSLDKSNFENTTSLG-------------------RENDTAIGILDRMTSILEEMQNPSTANSAFGPTLLLKDEENIGQFLYYEGIEYHMWNTYDVHFYASFALIMLFPKLELSIQRDFAAAVMMHDPERMKTLQDGKWVPRKVLGAVPHDLGLND--PWFEVNAYNIHDVNRWKDLNPKFVLQVYRDMVFTGDKSFAHSVWPSVYMAM-AYMDQFDKDRDGMIENEGFPDQTYDVWSVTGVSAYTGGLWVAALQAASAMAHEV-----GDNASA--DYFWHKFENAKRVY-GTLWNGSYFNYDNSDGAYSSSIQADQLAGQWYARACGLVPIVDDEKAQSALEKVYQFNVLKLGDGKRGAVNGMLPNGTVDMSTMQSREIWTGVTYAVAAAMIQEG------------------------MEDSAFKTAEGIYEASWSPEGLGYSFQTPEAWNTNGEFRSLSYMRPLAIWAIQWALSPPVLNNKTQKP 902          

HSP 2 Score: 179.104 bits (453), Expect = 2.645e-42
Identity = 108/308 (35.06%), Postives = 160/308 (51.95%), Query Frame = 0

Query:    4 LKESLSLLPLAWRMGQYVSGERRENRDPIFDLNGLLLQPPIPGPYA-GVPLGGLGGGCIGRGFRGEFRRWNLNPGKYRHKVVAANQFSVRVLRKGQAPVAQVLAVG-----DGRETDGLRAWRWGMDPRVGTYHALFPRAWTVYE-EAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRFS-SGGAGSKEASEVWRQFLEDGSLREI 303
            + E L +  L  RM ++++ E  + R  + D       P I      G+PLGG+G G IGR ++GEF+RW L PG    + V ANQFSV + R      + VL  G           G+ +W W ++    TYHAL+PRAWTVYE E  P +++ CRQ+SP++P++Y++SSLPV VF +T+ N G  AADVS++F+F N +G  S+ +G H N +  +                        V GV L H+    + +  +TFA+AA   +   VS C  F  SG +    A ++W +  E GS   +
Sbjct:   51 MLEKLKMASLGIRMWRHINEEHSKGRTAVIDP----FNPQIKHTSCHGIPLGGIGAGSIGRSYKGEFQRWQLFPGICEDEAVLANQFSVFISRPNGKSFSTVLHPGRPDVLKDHTISGIGSWDWNLNGEHSTYHALYPRAWTVYEDEPDPDLKIVCRQISPIIPNNYRESSLPVTVFTFTLINSGKDAADVSLLFTFANSVGGQSEFSGNHLNSQLRMMDG---------------------VRGVTLCHKVVNGQPE--VTFAIAAQETNDVHVSECPSFVISGNSQGFSARDMWHEVKEHGSFDRL 331          

BLAST of EWM29848.1 vs. NCBI_GenBank
Match: gi|727536804|ref|XP_010442466.1| (PREDICTED: non-lysosomal glucosylceramidase-like isoform X3 [Camelina sativa])

HSP 1 Score: 502.286 bits (1292), Expect = 1.425e-160
Identity = 283/597 (47.40%), Postives = 354/597 (59.30%), Query Frame = 0

Query:  399 PSKAKESIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWTE------SRAG---RAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDARRRAGGASGVENNRTFQSCHGEQ--RMVGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGWKR--FGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTR 982
            PS+   SI AAV  + ++ P     ++F+LAWD P V+F SG    RRY++F+G  G   A  IA  A+  +  WE  IEAWQRP+LED  LP +Y   LFNELY+L  GGTVWT+      S AG   +    D  + G +   T   R+ D    V            A  AS    N  F +   E+    +G FLY+EG EY M+NTYDVHFY+SFAL ML+P LELS+QRD AAAVM  D    + L  G+   RKV GAVPHDLG     PW +VN YNL + +RWKDL PK VLQ YRD +AT    FA AVWP + +V  A +  FD+DGDG+IEN GFPDQTYD W+  G SAY+GGLWVAAL A +AL   V   N          ++     +A+  Y   LWNG+YF+YDNSGSP+  SI ADQ+AGQWYARA GL P+    +AR++L  V+ +NV +  DG  GAVNGM P G+VD   MQS+E+W+G TY+++A M+QE                         L   AF+TA G+YEA W     GY F TPEAWN    +RSL YMRPL+IWAMQWALT+
Sbjct:  157 PSEHGSSIGAAVAASATVPPGESRIVTFSLAWDCPEVQFPSGKIYSRRYSKFYGNHGD-AAAQIAYDAILGHSQWESWIEAWQRPILEDKRLPEWYPITLFNELYYLNSGGTVWTDGSSPVHSLAGVREKKFSLDKSQLGLKNDTTDVPRQNDTAISVLEKMSSTLEELHASTAS----NSAFGTKLLEEGEENIGHFLYLEGVEYRMWNTYDVHFYSSFALVMLFPKLELSIQRDFAAAVMLHDPTKVKTLTEGQWVQRKVLGAVPHDLGIND--PWFEVNGYNLHNTDRWKDLNPKFVLQVYRDMVATGDKKFATAVWPSV-YVAMAYMAQFDKDGDGMIENEGFPDQTYDTWSASGVSAYSGGLWVAALQAASALARVVDDKNSQD-------YFWKKFQKAKVVYEKQLWNGSYFNYDNSGSPYSSSIQADQLAGQWYARASGLLPIVDEDKARTALEKVYNFNVMKIKDGKRGAVNGMHPSGKVDTASMQSREIWSGVTYALSATMIQEG------------------------LVDMAFQTASGVYEAAWSEGGLGYSFQTPEAWNTNDEYRSLTYMRPLAIWAMQWALTK 714          

HSP 2 Score: 99.7525 bits (247), Expect = 1.247e-17
Identity = 64/198 (32.32%), Postives = 99/198 (50.00%), Query Frame = 0

Query:  143 EAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRF-SSGGAGSKEASEVWRQFLEDGSLREIGRTE----EQHGDQDHLETLATRTR--GSDRIVETS 333
            E  P +R+ CRQVSP +PH+Y++SS PV VF +T+ NLG + AD +++F++ N +G  S+ +GGH+N +  ++                       V GV LHH+   A    +L++A++A +  G  VS C  F  SG      A ++W+   E+GS      +E     +HG        A+ T   G  RIV  S
Sbjct:   11 EPDPELRIVCRQVSPFIPHNYKESSFPVSVFTFTLHNLGDTTADATLLFTWANSVGGDSEFSGGHYNSKIMMNDG---------------------VQGVLLHHKT--AIGLPSLSYAISAQSTDGVSVSTCPFFIVSGKQNGITAKDMWQVIKENGSFDNFEASEVSMPSEHGSSIGAAVAASATVPPGESRIVTFS 185          

BLAST of EWM29848.1 vs. NCBI_GenBank
Match: gi|702375293|ref|XP_010062405.1| (PREDICTED: non-lysosomal glucosylceramidase isoform X3 [Eucalyptus grandis] >gi|702375297|ref|XP_010062406.1| PREDICTED: non-lysosomal glucosylceramidase isoform X3 [Eucalyptus grandis] >gi|1091482696|ref|XP_018731807.1| PREDICTED: non-lysosomal glucosylceramidase isoform X3 [Eucalyptus grandis] >gi|1091482698|ref|XP_018731808.1| PREDICTED: non-lysosomal glucosylceramidase isoform X3 [Eucalyptus grandis])

HSP 1 Score: 501.516 bits (1290), Expect = 8.180e-160
Identity = 279/599 (46.58%), Postives = 361/599 (60.27%), Query Frame = 0

Query:  399 PSKAKESIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWTESR-------AGRAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDARRRAGGASGVENNRTFQSCHGEQRM------VGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGW--KRFGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTR 982
            PS+A  SI AA+  ++ + P  V  ++F+LAWD P VRF   +   RRYT+F+G  G   A  IA  A+  +  WE +I+AWQ P+L D  LP++Y   LFNELY+L  GGT+WT+         +G    S  + + G   E G     D   E+          R A   S +    T  +  G   +      +GQFLY+EG+EYLM+NTYDVHFY+SFAL ML+P LELS+QRD AAAVM  D   R++++ G+  PRKV GAVPHD+G  +  PW +VNAYNL + +RWKDL PK VLQ YRD++AT    FA+AVWP +   M A +  FD+DGDG+IEN GFPDQTYD W+V G SAY GGLWVAAL A +A+   V     G        +Y  +  Q  +     LWNG Y++YD+SGS    SI ADQ+AGQW ARACGL P+A   + RS+L  VF +NV +   G  GAVNGM PDG++D + MQS+EVW G TYS+AA+M+QE  +                          AF+TA G+YEA W  K  G+ F TPEAWN    FRS+ YMRPL+IWAMQWAL++
Sbjct:  204 PSEAGSSIGAAIAASLVVPPGSVRTVTFSLAWDCPEVRFDDNVYY-RRYTKFYGTNGD-AAAKIARDAILEHTQWESQIDAWQGPILHDRRLPSWYPVTLFNELYYLNSGGTIWTDGSPPVRNLLSGHVKFSLDQAKLGLKNEVGN----DTAVEI--------IERMASTLSRIHGPLTSNTAFGTSLLQEGEENIGQFLYLEGNEYLMWNTYDVHFYSSFALIMLYPKLELSIQRDFAAAVMLHDPQKRKIMSDGKWVPRKVLGAVPHDIGLNN--PWFEVNAYNLFNTDRWKDLNPKFVLQVYRDFIATGDLNFAKAVWPSVYTAM-AYMDQFDKDGDGMIENEGFPDQTYDAWSVSGVSAYCGGLWVAALQAASAMAREVGDITSG--------NYFWVKYQKAKVVYDKLWNGCYYNYDDSGSSVSASIQADQLAGQWIARACGLLPIADSDKIRSALEKVFDFNVSKMKGGTRGAVNGMLPDGRIDMSAMQSREVWPGVTYSLAASMIQEGMM------------------------DMAFQTAAGVYEAAWSEKSLGFSFQTPEAWNENDEFRSICYMRPLAIWAMQWALSK 753          

HSP 2 Score: 114.005 bits (284), Expect = 4.078e-22
Identity = 71/201 (35.32%), Postives = 108/201 (53.73%), Query Frame = 0

Query:  110 GRETDGLRAWRWGMDPRVGTYHALFPRAWTVYE-EAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRF-SSGGAGSKEASEVWRQFLEDGSLREIGRTEE 308
            G    G   W W ++    TYHALFPRAWT+ + E  P +R+  RQ+SP++PH+Y++SS PV VF +TV N G ++ADVS++F++ N +G  S  +GGHFN                    +  E  +G + G+ LHH+   A     +TFA+AA+  S   +S C  F  SG +    A ++W    ++GS    G+ + 
Sbjct:   24 GSRGSGTECWDWNLNGDKCTYHALFPRAWTICDGEPDPDLRIVTRQLSPIIPHNYKESSFPVSVFTFTVSNQGRTSADVSLLFTWVNSVGGVSGFSGGHFN--------------------SNMETTDG-IHGILLHHRT--ANGHPPVTFAIAAEESSDVHISKCPYFLISGTSHGFTAKDMWYAIKKNGSFDHTGQNDN 201          

BLAST of EWM29848.1 vs. NCBI_GenBank
Match: gi|1073020358|ref|XP_018461486.1| (PREDICTED: non-lysosomal glucosylceramidase-like isoform X2 [Raphanus sativus])

HSP 1 Score: 503.442 bits (1295), Expect = 7.764e-159
Identity = 282/588 (47.96%), Postives = 351/588 (59.69%), Query Frame = 0

Query:  405 SIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWTE------SRAGRAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDARRRAGGASGVENNRTFQSCHGEQ--RMVGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGWKR--FGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTR 982
            SI AAV  + ++ P     ++F+LAWD P V+F SG    RRYT+F+G +G   A  IA  A+  +  WE  IE WQRP+LED  LPA+Y   LFNELY+L  GGT+WT+      S AG A E     +  Q         P Q+ +      E+ A       +   +N  F +   E+    +G FLY+EG EY M+NTYDVHFYASFAL ML+P LELS+QRD AAAVM  D    + L+ G+   RKV GAVPHDLG     PW +VN YNL + +RWKDL PK VLQ YRD +AT    FA AVWP + +V  A +  FD+DGDG+IEN GFPDQTYD W+  G SAY GGLWVAAL A +AL   V   N          ++ +   +A+  Y   LWNG+YF YDNSGS +  SI ADQ+AGQWYARA GL P+    +AR +L  VF +NV +  DG  GAVNGM PDG+VD   MQS+E+W+G TY+++A M+QE                         L   AF+TA G+YEA W     GY F TPEAWN    +RSL YMRPL+IWAMQWALTR
Sbjct:  342 SIGAAVAASATVLPGQSRIITFSLAWDCPEVQFPSGKIYSRRYTKFYGTDGN-AAAQIAYDAILGHSQWESWIEDWQRPILEDKRLPAWYPITLFNELYYLNSGGTLWTDGSSPLHSLAG-AREKKFSLDKSQSSLKNIIDVPQQQNDTAVSVLEKMASTLEQLHASTASNSAFGTKLLEEGEENIGHFLYLEGIEYRMWNTYDVHFYASFALVMLFPKLELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKVLGAVPHDLGIND--PWFEVNGYNLHNTDRWKDLNPKFVLQVYRDVVATGDKKFALAVWPSV-YVAMAYMAQFDKDGDGMIENEGFPDQTYDTWSASGVSAYCGGLWVAALQAASALAREVGDKNSQD-------YFWSKFQKAKVVYEKKLWNGSYFSYDNSGSQYSSSIQADQLAGQWYARASGLMPIVDEDKARKALEKVFNFNVMKIKDGKRGAVNGMHPDGKVDTASMQSREIWSGVTYALSATMIQEG------------------------LVDKAFQTASGVYEAAWSETGLGYSFQTPEAWNTNDQYRSLTYMRPLAIWAMQWALTR 893          

HSP 2 Score: 165.622 bits (418), Expect = 4.526e-38
Identity = 106/297 (35.69%), Postives = 156/297 (52.53%), Query Frame = 0

Query:   50 GVPLGGLGGGCIGRGFRGEFRRWNLNPGKYRHKVVAANQFSVRVLRKGQAPVAQVLA-----VGDGRETDGLRAWRWGMDPRVGTYHALFPRAWTVYE-EAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRFS-SGGAGSKEASEVWRQFLEDGSLREIGRTE----EQHGDQ--DHLETLATRTRGSDRIVETS 333
            GVPLGG+G G IGR F+GEF+RW L P K   + V ANQFS  V R      + VL      +       G+ +W W +     TYHAL+PR+WT+YE E  P +R+ CRQ+SP +PH+Y++SS PV VF +T+ NLG + AD +++F++ N +G  S+ +GGH+N +  ++                    +G V GV LHH+   A    +L++A+ A    G  +S+C  F+ SG      A ++W    E GS   +  +E     +HG      +   AT   G  RI+  S
Sbjct:   91 GVPLGGIGSGSIGRSFKGEFQRWQLFPPKCEDEPVLANQFSAFVSRANGKKYSSVLCPRNPKLAKQESEPGISSWDWNLKGDKSTYHALYPRSWTIYEGEPDPELRIVCRQISPFIPHNYKESSFPVSVFTFTIHNLGDTTADATLLFTWANSVGGDSEFSGGHYNSKIMMN--------------------DG-VKGVLLHHKT--ANGLPSLSYAITAQETDGVSISVCPFFTVSGKQNGITAKDMWNIIKEHGSFDHLDASEASMQSEHGSSIGAAVAASATVLPGQSRIITFS 364          

BLAST of EWM29848.1 vs. NCBI_GenBank
Match: gi|1073019464|ref|XP_018461028.1| (PREDICTED: non-lysosomal glucosylceramidase-like isoform X2 [Raphanus sativus] >gi|1073019466|ref|XP_018461029.1| PREDICTED: non-lysosomal glucosylceramidase-like isoform X2 [Raphanus sativus])

HSP 1 Score: 503.442 bits (1295), Expect = 9.848e-159
Identity = 282/588 (47.96%), Postives = 351/588 (59.69%), Query Frame = 0

Query:  405 SIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWTE------SRAGRAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDARRRAGGASGVENNRTFQSCHGEQ--RMVGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGWKR--FGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTR 982
            SI AAV  + ++ P     ++F+LAWD P V+F SG    RRYT+F+G +G   A  IA  A+  +  WE  IE WQRP+LED  LPA+Y   LFNELY+L  GGT+WT+      S AG A E     +  Q         P Q+ +      E+ A       +   +N  F +   E+    +G FLY+EG EY M+NTYDVHFYASFAL ML+P LELS+QRD AAAVM  D    + L+ G+   RKV GAVPHDLG     PW +VN YNL + +RWKDL PK VLQ YRD +AT    FA AVWP + +V  A +  FD+DGDG+IEN GFPDQTYD W+  G SAY GGLWVAAL A +AL   V   N          ++ +   +A+  Y   LWNG+YF YDNSGS +  SI ADQ+AGQWYARA GL P+    +AR +L  VF +NV +  DG  GAVNGM PDG+VD   MQS+E+W+G TY+++A M+QE                         L   AF+TA G+YEA W     GY F TPEAWN    +RSL YMRPL+IWAMQWALTR
Sbjct:  341 SIGAAVAASATVLPGESRIITFSLAWDCPEVQFPSGKIYSRRYTKFYGTDGN-AAAQIAYDAILGHSQWESWIEDWQRPILEDKRLPAWYPITLFNELYYLNSGGTLWTDGSSPLHSLAG-AREKKFSLDKSQSSLKNIIDVPQQQNDTAVSVLEKMASTLEQLHASTASNSAFGTKLLEEGEENIGHFLYLEGIEYRMWNTYDVHFYASFALVMLFPKLELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKVLGAVPHDLGIND--PWFEVNGYNLHNTDRWKDLNPKFVLQVYRDVVATGDKKFALAVWPSV-YVAMAYMAQFDKDGDGMIENEGFPDQTYDTWSASGVSAYCGGLWVAALQAASALAREVGDKNSQD-------YFWSKFQKAKVVYEKKLWNGSYFSYDNSGSQYSSSIQADQLAGQWYARASGLMPIVDEDKARKALEKVFNFNVMKIKDGKRGAVNGMHPDGKVDTASMQSREIWSGVTYALSATMIQEG------------------------LVDKAFQTASGVYEAAWSETGLGYSFQTPEAWNTNDQYRSLTYMRPLAIWAMQWALTR 892          

HSP 2 Score: 165.622 bits (418), Expect = 4.357e-38
Identity = 106/297 (35.69%), Postives = 157/297 (52.86%), Query Frame = 0

Query:   50 GVPLGGLGGGCIGRGFRGEFRRWNLNPGKYRHKVVAANQFSVRVLRKGQAPVAQVLA-----VGDGRETDGLRAWRWGMDPRVGTYHALFPRAWTVYE-EAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRFS-SGGAGSKEASEVWRQFLEDGSLREIGRTE----EQHGDQ--DHLETLATRTRGSDRIVETS 333
            GVPLGG+G G IGR F+GEF+RW L P K   + V AN+FS  V R      + VL      +       G+ +W W +     TYHAL+PR+WT+YE E  P +R+ CRQ+SP +PH+Y++SS PV VF +T+ NLG + AD +++F++ N +G  S+ +GGH+N +  ++                    +G V GV LHH+   A    +L++A++A    G  VS+C  F+ SG      A ++W    E GS   +  +E     +HG      +   AT   G  RI+  S
Sbjct:   90 GVPLGGIGSGSIGRSFKGEFQRWQLFPPKCEDEPVLANEFSAFVSRANGKKYSSVLCPRNPKLAKQESESGISSWDWNLKGDKSTYHALYPRSWTIYEGEPDPELRIVCRQISPFIPHNYKESSFPVSVFTFTIHNLGETTADATLLFTWANSVGGDSEFSGGHYNSKIMMN--------------------DG-VKGVLLHHKT--ANGLPSLSYAISAQETDGVSVSVCPFFTVSGKQNGITAKDMWNIIKEHGSFDHLDASEASMQSEHGSSIGAAVAASATVLPGESRIITFS 363          

BLAST of EWM29848.1 vs. NCBI_GenBank
Match: gi|1073019460|ref|XP_018461026.1| (PREDICTED: non-lysosomal glucosylceramidase-like isoform X1 [Raphanus sativus] >gi|1073019462|ref|XP_018461027.1| PREDICTED: non-lysosomal glucosylceramidase-like isoform X1 [Raphanus sativus])

HSP 1 Score: 503.056 bits (1294), Expect = 1.078e-158
Identity = 282/588 (47.96%), Postives = 351/588 (59.69%), Query Frame = 0

Query:  405 SIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWTE------SRAGRAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDARRRAGGASGVENNRTFQSCHGEQ--RMVGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGWKR--FGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTR 982
            SI AAV  + ++ P     ++F+LAWD P V+F SG    RRYT+F+G +G   A  IA  A+  +  WE  IE WQRP+LED  LPA+Y   LFNELY+L  GGT+WT+      S AG A E     +  Q         P Q+ +      E+ A       +   +N  F +   E+    +G FLY+EG EY M+NTYDVHFYASFAL ML+P LELS+QRD AAAVM  D    + L+ G+   RKV GAVPHDLG     PW +VN YNL + +RWKDL PK VLQ YRD +AT    FA AVWP + +V  A +  FD+DGDG+IEN GFPDQTYD W+  G SAY GGLWVAAL A +AL   V   N          ++ +   +A+  Y   LWNG+YF YDNSGS +  SI ADQ+AGQWYARA GL P+    +AR +L  VF +NV +  DG  GAVNGM PDG+VD   MQS+E+W+G TY+++A M+QE                         L   AF+TA G+YEA W     GY F TPEAWN    +RSL YMRPL+IWAMQWALTR
Sbjct:  342 SIGAAVAASATVLPGESRIITFSLAWDCPEVQFPSGKIYSRRYTKFYGTDGN-AAAQIAYDAILGHSQWESWIEDWQRPILEDKRLPAWYPITLFNELYYLNSGGTLWTDGSSPLHSLAG-AREKKFSLDKSQSSLKNIIDVPQQQNDTAVSVLEKMASTLEQLHASTASNSAFGTKLLEEGEENIGHFLYLEGIEYRMWNTYDVHFYASFALVMLFPKLELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKVLGAVPHDLGIND--PWFEVNGYNLHNTDRWKDLNPKFVLQVYRDVVATGDKKFALAVWPSV-YVAMAYMAQFDKDGDGMIENEGFPDQTYDTWSASGVSAYCGGLWVAALQAASALAREVGDKNSQD-------YFWSKFQKAKVVYEKKLWNGSYFSYDNSGSQYSSSIQADQLAGQWYARASGLMPIVDEDKARKALEKVFNFNVMKIKDGKRGAVNGMHPDGKVDTASMQSREIWSGVTYALSATMIQEG------------------------LVDKAFQTASGVYEAAWSETGLGYSFQTPEAWNTNDQYRSLTYMRPLAIWAMQWALTR 893          

HSP 2 Score: 165.622 bits (418), Expect = 4.811e-38
Identity = 106/297 (35.69%), Postives = 157/297 (52.86%), Query Frame = 0

Query:   50 GVPLGGLGGGCIGRGFRGEFRRWNLNPGKYRHKVVAANQFSVRVLRKGQAPVAQVLA-----VGDGRETDGLRAWRWGMDPRVGTYHALFPRAWTVYE-EAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRFS-SGGAGSKEASEVWRQFLEDGSLREIGRTE----EQHGDQ--DHLETLATRTRGSDRIVETS 333
            GVPLGG+G G IGR F+GEF+RW L P K   + V AN+FS  V R      + VL      +       G+ +W W +     TYHAL+PR+WT+YE E  P +R+ CRQ+SP +PH+Y++SS PV VF +T+ NLG + AD +++F++ N +G  S+ +GGH+N +  ++                    +G V GV LHH+   A    +L++A++A    G  VS+C  F+ SG      A ++W    E GS   +  +E     +HG      +   AT   G  RI+  S
Sbjct:   91 GVPLGGIGSGSIGRSFKGEFQRWQLFPPKCEDEPVLANEFSAFVSRANGKKYSSVLCPRNPKLAKQESESGISSWDWNLKGDKSTYHALYPRSWTIYEGEPDPELRIVCRQISPFIPHNYKESSFPVSVFTFTIHNLGETTADATLLFTWANSVGGDSEFSGGHYNSKIMMN--------------------DG-VKGVLLHHKT--ANGLPSLSYAISAQETDGVSVSVCPFFTVSGKQNGITAKDMWNIIKEHGSFDHLDASEASMQSEHGSSIGAAVAASATVLPGESRIITFS 364          

BLAST of EWM29848.1 vs. NCBI_GenBank
Match: gi|702375261|ref|XP_010062399.1| (PREDICTED: non-lysosomal glucosylceramidase isoform X1 [Eucalyptus grandis] >gi|702375265|ref|XP_010062400.1| PREDICTED: non-lysosomal glucosylceramidase isoform X1 [Eucalyptus grandis] >gi|702375270|ref|XP_010062401.1| PREDICTED: non-lysosomal glucosylceramidase isoform X1 [Eucalyptus grandis] >gi|702375276|ref|XP_010062402.1| PREDICTED: non-lysosomal glucosylceramidase isoform X1 [Eucalyptus grandis] >gi|629104075|gb|KCW69544.1| hypothetical protein EUGRSUZ_F02980 [Eucalyptus grandis])

HSP 1 Score: 502.671 bits (1293), Expect = 1.465e-158
Identity = 279/599 (46.58%), Postives = 361/599 (60.27%), Query Frame = 0

Query:  399 PSKAKESIAAAVCQAVSLGPHGVARLSFALAWDMPIVRFGSGLALPRRYTRFFGKEGGLTAGLIASHALRYYPLWEKEIEAWQRPVLEDPSLPAFYKHMLFNELYFLTDGGTVWTESR-------AGRAHESDGEEEGGQGWETGRNRRPDQEGEVWRGNREQDARRRAGGASGVENNRTFQSCHGEQRM------VGQFLYVEGHEYLMYNTYDVHFYASFALTMLWPMLELSVQRDVAAAVMGQDLGMRRLLARGEMRPRKVAGAVPHDLGCPSGVPWGKVNAYNLQDVNRWKDLGPKLVLQAYRDYMATESAPFARAVWPVLRHVMEAALTAFDRDGDGLIENSGFPDQTYDIWTVKGPSAYTGGLWVAALTAMAALGEAVTTTNGGSDAQAVVAHYQALATQAREAYAGHLWNGTYFDYDNSGSPHQDSIMADQMAGQWYARACGLTPVAKGAQARSSLTTVFKYNVQQFADGCSGAVNGMRPDGQVDDTCMQSQEVWTGTTYSVAAAMLQEAALAASEAAEHSEGRGRALQEEADFLRHAAFETARGLYEAGW--KRFGYWFATPEAWNHEGHFRSLGYMRPLSIWAMQWALTR 982
            PS+A  SI AA+  ++ + P  V  ++F+LAWD P VRF   +   RRYT+F+G  G   A  IA  A+  +  WE +I+AWQ P+L D  LP++Y   LFNELY+L  GGT+WT+         +G    S  + + G   E G     D   E+          R A   S +    T  +  G   +      +GQFLY+EG+EYLM+NTYDVHFY+SFAL ML+P LELS+QRD AAAVM  D   R++++ G+  PRKV GAVPHD+G  +  PW +VNAYNL + +RWKDL PK VLQ YRD++AT    FA+AVWP +   M A +  FD+DGDG+IEN GFPDQTYD W+V G SAY GGLWVAAL A +A+   V     G        +Y  +  Q  +     LWNG Y++YD+SGS    SI ADQ+AGQW ARACGL P+A   + RS+L  VF +NV +   G  GAVNGM PDG++D + MQS+EVW G TYS+AA+M+QE  +                          AF+TA G+YEA W  K  G+ F TPEAWN    FRS+ YMRPL+IWAMQWAL++
Sbjct:  337 PSEAGSSIGAAIAASLVVPPGSVRTVTFSLAWDCPEVRFDDNVYY-RRYTKFYGTNGD-AAAKIARDAILEHTQWESQIDAWQGPILHDRRLPSWYPVTLFNELYYLNSGGTIWTDGSPPVRNLLSGHVKFSLDQAKLGLKNEVGN----DTAVEI--------IERMASTLSRIHGPLTSNTAFGTSLLQEGEENIGQFLYLEGNEYLMWNTYDVHFYSSFALIMLYPKLELSIQRDFAAAVMLHDPQKRKIMSDGKWVPRKVLGAVPHDIGLNN--PWFEVNAYNLFNTDRWKDLNPKFVLQVYRDFIATGDLNFAKAVWPSVYTAM-AYMDQFDKDGDGMIENEGFPDQTYDAWSVSGVSAYCGGLWVAALQAASAMAREVGDITSG--------NYFWVKYQKAKVVYDKLWNGCYYNYDDSGSSVSASIQADQLAGQWIARACGLLPIADSDKIRSALEKVFDFNVSKMKGGTRGAVNGMLPDGRIDMSAMQSREVWPGVTYSLAASMIQEGMM------------------------DMAFQTAAGVYEAAWSEKSLGFSFQTPEAWNENDEFRSICYMRPLAIWAMQWALSK 886          

HSP 2 Score: 169.088 bits (427), Expect = 4.090e-39
Identity = 111/311 (35.69%), Postives = 162/311 (52.09%), Query Frame = 0

Query:    4 LKESLSLLPLAWRMGQYVSGERRENRDPIFDLNGLLLQPPIPGPYAGVPLGGLGGGCIGRGFRGEFRRWNLNPGKYRHKVVAANQFSVRVLRKGQAPVAQVLA-----VGDGRETDGLRAWRWGMDPRVGTYHALFPRAWTVYE-EAVPLVRLTCRQVSPVLPHDYQDSSLPVCVFEWTVENLGASAADVSIMFSFQNGLGTSSDGAGGHFNRRFSVHRQDSSPRGGEQEAGTTEEAEEGVVVGVHLHHQQEGARDKDALTFAVAADAQSGSRVSLCSRF-SSGGAGSKEASEVWRQFLEDGSLREIGRTE 307
            L E L L P+  R+  +   E    RD IFD      +  + G + GVPLGG+G G IGR ++GEF+R+ L P       + ANQFSV V R      + VL      +  G    G   W W ++    TYHALFPRAWT+ + E  P +R+  RQ+SP++PH+Y++SS PV VF +TV N G ++ADVS++F++ N +G  S  +GGHFN                    +  E  +G + G+ LHH+   A     +TFA+AA+  S   +S C  F  SG +    A ++W    ++GS    G+ +
Sbjct:   50 LHEKLHLAPIGVRLWHHSKQEAVHGRDTIFDFTK---KRHVTG-HQGVPLGGIGAGSIGRSYKGEFQRFQLFPKVSEDVPILANQFSVMVSRPNGKKFSTVLCPKTAGMPKGSRGSGTECWDWNLNGDKCTYHALFPRAWTICDGEPDPDLRIVTRQLSPIIPHNYKESSFPVSVFTFTVSNQGRTSADVSLLFTWVNSVGGVSGFSGGHFN--------------------SNMETTDG-IHGILLHHRT--ANGHPPVTFAIAAEESSDVHISKCPYFLISGTSHGFTAKDMWYAIKKNGSFDHTGQND 333