EWM30567.1, cds84 (CDS) Nannochloropsis gaditana

BLAST of EWM30567.1 vs. NCBI_GenBank
Match: gi|585113234|gb|EWM30567.1| (tubulin--tyrosine ligase-like protein [Nannochloropsis gaditana])

HSP 1 Score: 1449.11 bits (3750), Expect = 0.000e+0
Identity = 698/698 (100.00%), Postives = 698/698 (100.00%), Query Frame = 0

Query:    1 MDASATSSPPPSSFLDRHRKQLESVGFPPTLYSRLESKLVGETFDAGDTFGVVEDESQRRAVVCSAANGLEPQCDVWLIDHLYSFQVETYRQDLQKRPELLSRLAVMMGLEEPHEDGIFEEETPETSMEKNPIPPLASVPTPGLRAEKRPDSLNLNEETLCLRRPHRRERPTLYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPSTALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAGYTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFSFYGVDVMVTKDLQPKILECTFGPDCASALKLDADFYNKAFGIIFRGEEYEDFRQL 698
            MDASATSSPPPSSFLDRHRKQLESVGFPPTLYSRLESKLVGETFDAGDTFGVVEDESQRRAVVCSAANGLEPQCDVWLIDHLYSFQVETYRQDLQKRPELLSRLAVMMGLEEPHEDGIFEEETPETSMEKNPIPPLASVPTPGLRAEKRPDSLNLNEETLCLRRPHRRERPTLYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPSTALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAGYTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFSFYGVDVMVTKDLQPKILECTFGPDCASALKLDADFYNKAFGIIFRGEEYEDFRQL
Sbjct:    1 MDASATSSPPPSSFLDRHRKQLESVGFPPTLYSRLESKLVGETFDAGDTFGVVEDESQRRAVVCSAANGLEPQCDVWLIDHLYSFQVETYRQDLQKRPELLSRLAVMMGLEEPHEDGIFEEETPETSMEKNPIPPLASVPTPGLRAEKRPDSLNLNEETLCLRRPHRRERPTLYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPSTALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAGYTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFSFYGVDVMVTKDLQPKILECTFGPDCASALKLDADFYNKAFGIIFRGEEYEDFRQL 698          

BLAST of EWM30567.1 vs. NCBI_GenBank
Match: gi|659100091|ref|XP_008450923.1| (PREDICTED: tubulin--tyrosine ligase-like protein 12 [Cucumis melo])

HSP 1 Score: 291.967 bits (746), Expect = 1.480e-83
Identity = 190/573 (33.16%), Postives = 294/573 (51.31%), Query Frame = 0

Query:  133 IPPLASVPTPGLRAEKRPDS-LNLNEETLCLRRPHRRERPTLYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPSTALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAGYTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFS--FYGVDVMVTKDLQPKILECTFGPDCASALKLDA------------DFYNKAFGIIFRGE 690
            +P L+++   G+  EK   S  ++ +  L  R P      TL ++V   +W+Y+ +YRL D  +     +VW+V DE GSA +H+     NFR++PF+ F P G+L  A+ +++ WPI N+ +G+ECT DY FG           E+K     +R+ RL  WF  P +        H   K +        N  SK +  P +   +      +     LRVY DN QV   L+R +    ++P EAD++W     I    R+  G+     V+Q   + CLV KH L  T  +A G   WL  TYN+ET  +  I +Y  R     R G                 L+ L+ILKPWN+ R++D+ V D+L   ++L +T PK+  +Y+   A  N +KFD+RY + VR+ +PLEIF    + VR++N+PYSL  +++ +++ HFTVM+Y G      +  DF+  FE+++  V W ++ S+V  +I+++FE+AA++        E+   FS   YG+DVM+    QPK+LE T+ PDC  A K D             +FYN  FG +F  E
Sbjct:  354 LPNLSNLN--GIDVEKILSSGKHMIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADE-EKIDETSVWYVMDELGSALRHSDEP--NFRVAPFL-FMPEGNLTSAISFTILWPIHNVQKGDECTRDYLFGIG---------EDK-----QRSARLTAWFHTPQNY-----FVHEYEKHI-------KNLQSKVLTSPISQTTSKTEELRQSKGGTLRVYTDNPQVEEFLNRPEFTITSDPKEADIIWT-SMQIDEDTRKATGITDKQYVNQFPFEACLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRK----RDG-----------------LNNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHK-NIADFVREFEQEH-NVKWLDIHSRVTSMIRSVFESAAVV------HPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVENVFGGGVIEGREFYNYIFGCLFLNE 864          

BLAST of EWM30567.1 vs. NCBI_GenBank
Match: gi|449452568|ref|XP_004144031.1| (PREDICTED: tubulin--tyrosine ligase-like protein 12 [Cucumis sativus] >gi|700211204|gb|KGN66300.1| hypothetical protein Csa_1G596500 [Cucumis sativus])

HSP 1 Score: 291.197 bits (744), Expect = 3.250e-83
Identity = 181/542 (33.39%), Postives = 278/542 (51.29%), Query Frame = 0

Query:  163 RRPHRRERPTLYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPSTALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAGYTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFS--FYGVDVMVTKDLQPKILECTFGPDCASALKLDAD------------FYNKAFGIIFRGE 690
            R P      TL ++V   +W+Y+ +YRL D  +     +VW+V DE GSA +H+     NFR++PF+ F P G+L  A+ +++ WPI N+ +G+ECT DY FG           E+K     +R+ RL  WF  P +        H   K +        N  SK +  P +   +      +     LRVY DN QV   L+R +    ++P EAD++W     I    R+  G+     V+Q   + CLV KH L  T  +A G   WL  TYN+ET  +  I +Y  R  +                      L+ L+ILKPWN+ R++D+ V D+L   ++L +T PK+  +Y+   A  N +KFD+RY + VR+ +PLEIF    + VR++N+PYSL  +++ +++ HFTVM+Y G      +  DF+  FE+++  V W ++ S+V  +I+++FE+AA++        E+   FS   YG+DVM+    QPK+LE T+ PDC  A K D +            FYN  FG +F  E
Sbjct:  387 RLPEWAPEETLPDRVINAMWQYLMTYRLADE-EKIDETSVWYVMDELGSALRHSDEP--NFRVAPFL-FMPEGNLMSAISFTILWPIHNVQKGDECTRDYLFGIG---------EDK-----QRSARLTAWFHTPQNY-----FVHEYEKHI-------KNLQSKVLTSPISQTTSKTEELCQSKGGTLRVYTDNPQVEEFLNRPEFTITSDPKEADIIWT-SMQIDEDTRKATGITDKQYVNQFPFEACLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKRDR---------------------LNNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHK-NIADFVREFEQEH-NVKWLDIHSRVRSMIRSVFESAAVV------HPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVENVFGGEIIKGEGFYNYIFGCLFLNE 868          

BLAST of EWM30567.1 vs. NCBI_GenBank
Match: gi|1026061266|ref|XP_016575081.1| (PREDICTED: tubulin--tyrosine ligase-like protein 12 [Capsicum annuum])

HSP 1 Score: 286.189 bits (731), Expect = 1.556e-81
Identity = 179/534 (33.52%), Postives = 278/534 (52.06%), Query Frame = 0

Query:  173 LYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPSTALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAG---YTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFSFYGVDVMVTKDLQPKILECTFGPDCASALKLDA-------------DFYNKAFGIIFRGE 690
            L ++V   +W Y+ +YRL D  +     +VW+V DE GSA +H+     NFR+SPF+Y P  G+L  A+ YS+ WPI ++ EG+ECT DY FG           EEK     +R+ RL  WF  P   R  F   + + K    S+ ++      P+  P +++    S   R     LRVY D  QV  +L+R +     EP +AD++W     I    ++  G+  +  ++Q   + CLV KH L  T  +A G   WL  TYN+ET+ +  I ++  R  E                      L  L+ILKPWN+ R++D+ +  +L   ++L +T PK++ +Y+   A +N RKFD+RY + VR+  PLEIF    + VR++N+ Y+L   + ++++ HFTVM+Y G   +  TP    DF++ FE+++  V W ++ S++  +IK+ F+ AA +  E       ++S + YGVDVM+    QPK+LE T+ PDC  A+  D              DFYN  FG +F  E
Sbjct:  388 LTDRVINAMWLYLMTYRLADE-EKIDETSVWYVMDELGSALRHSDKP--NFRVSPFLYMPE-GNLASAVSYSILWPIDDVREGDECTRDYLFGIG---------EEK-----QRSARLTAWFHTP---RNYFVKEYEKYKNKLQSIKIA-----SPI--PGSSIT---SSPCRGDGRALRVYADIPQVEEYLTRPEFVITTEPKDADIIWT-SMQIDEETKKTTGINDEQYINQFPFEACLVMKHHLAETIQKAHGLVEWLQPTYNLETQLSQLIGDFHLRESEK---------------------LDNLWILKPWNMARTIDTTITSNLSAIIRLMETGPKISQKYIEHPALLNGRKFDLRYIVLVRSMNPLEIFLAEVFWVRLANNTYTLEKHSFDEYETHFTVMNYRGRLNHMNTP----DFVKEFEKEH-EVNWQDIHSRIRNMIKSAFKAAAAVHPEM----HHSKSRAMYGVDVMLDCHFQPKLLEITYCPDCTRAVTYDTEAVVGGGETVKGKDFYNYIFGCLFLNE 859          

BLAST of EWM30567.1 vs. NCBI_GenBank
Match: gi|661893276|emb|CDP03085.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 286.189 bits (731), Expect = 2.104e-81
Identity = 185/529 (34.97%), Postives = 277/529 (52.36%), Query Frame = 0

Query:  175 EQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPSTALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAGYTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFSFYGVDVMVTKDLQPKILECTFGPDCASALKLD-------------ADFYNKAFGIIFRGE 690
            ++V   +W ++ +YRL D  +     +VW+V DE GSA +H+     NF +SPF+Y P  G L  A+ YS+ WPIANI  G+ECT D+ FG           EEK     +R+ RL  WF  P +    +     E+++L L    S      PV   ST  A + S         LRVY D  QV   L+R +    +EP +AD++W     +    R+  GL     ++Q   + CLV KH L  T  +A GS  WL  TYN+ETE    I +Y  R  E                      L+ L+ILKPWN+ R++D+ V  +L   ++L +T PKV  +Y+   A +N RKFD+RY + VR+  PLEIF    + VR++N+ YSL   ++ +++ HFTVM+Y G     ++ ++F++ FE+++  V W ++ S+V ++I+++FE+AA +  E     +   S + YGVDVM+ +  QPKILE T+ PDC  A K D             +DF+N  FG +F  E
Sbjct:  400 DRVISAMWLFLMTYRLADE-EKVDETSVWYVMDELGSALRHSDEP--NFSVSPFLYLP-GGDLASAVSYSIVWPIANIERGDECTRDFLFGIG---------EEK-----QRSARLTAWFHTPQN----YFIREYEKQRLKLQ---SKKFHPLPVVS-STTKALYGSDG-----TALRVYTDIPQVEEFLTRPEFIITSEPKDADIIWT-SMQVDEEMRKAVGLNEQQFINQFPFEACLVMKHHLAETIQKAHGSPEWLQPTYNLETELTQLIGDYCIRERER---------------------LNNLWILKPWNMARTIDTTVTGNLSAIIRLMETGPKVCQKYIEHPALLNGRKFDIRYIVLVRSMNPLEIFLADTFWVRLANNTYSLDKHSLFEYETHFTVMNYRGRLNH-MNTSEFVKEFEKEH-HVNWMDIHSRVSRMIRSIFESAAAVHPEM----QSPTSRAIYGVDVMLDRHFQPKILEVTYCPDCTRACKYDTEPVIRAGGFVKGSDFFNYVFGCLFLNE 869          

BLAST of EWM30567.1 vs. NCBI_GenBank
Match: gi|698571254|ref|XP_009774830.1| (PREDICTED: tubulin--tyrosine ligase-like protein 12 [Nicotiana sylvestris])

HSP 1 Score: 285.034 bits (728), Expect = 4.371e-81
Identity = 182/535 (34.02%), Postives = 274/535 (51.21%), Query Frame = 0

Query:  173 LYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPSTALA-AHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAG---YTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFSFYGVDVMVTKDLQPKILECTFGPDCASALKLDA-------------DFYNKAFGIIFRGE 690
            L ++V   +W Y+ +YRL D  +     +VW+V DE GSA +H+     NFR+SPF+Y P  G+L  A+ YS+ WPI ++ EG+ECT DY FG           EEK     +R+ RL  WF  P   R  F   + + K    S     N    PV   S  ++  H   S       LRVY D   V  +L+R +     EP +AD++W     I    ++  GL  +  ++Q   + CLV KH L  T  +A G   WL  TYN+ET+    I ++ +R  E                      L  L+ILKPWN+ R++D+ +  +L   ++L +T PK+  +Y+   A    RKFD+RY + VR+  PLEIF    + VR++N+ Y+L   + ++++ HFTVM+Y G   +  TP    DF++ FE+++  V W ++ S++ ++IK+ FE AA++  E       ++S + YGVDVM+    QPK+LE T+ PDC  A+K D              DFYN  FG +F  E
Sbjct:  382 LEDRVINAMWLYLMTYRLADE-EKIDETSVWYVMDELGSALRHSDKP--NFRVSPFLYMP-EGNLASAVSYSIIWPIDDVREGDECTRDYLFGIG---------EEK-----QRSARLTAWFHTP---RNYFIKEYEKYKNKLQS-----NKFVSPVQGSSVTISLCHGDGS------ALRVYTDIPPVEEYLTRPEFVITTEPKDADIIWT-SMQIDEETKKAAGLNDEQYINQFPFEACLVMKHHLAETIQKAHGLVEWLQPTYNLETQLTQLIGDFHQREREK---------------------LDNLWILKPWNMARTIDTTITSNLSAIIRLMETGPKICQKYIEHPALFKGRKFDLRYIVLVRSMNPLEIFLAEVFWVRLANNTYTLEKHSFDEYETHFTVMNYRGRLNHMNTP----DFVKEFEKEH-KVNWLDIHSRIRRMIKSAFEAAAVVHPEM----HHSKSRAMYGVDVMLDCHFQPKLLEITYCPDCGRAVKYDTEAVVGGGETVKGKDFYNYIFGCLFLNE 853          

BLAST of EWM30567.1 vs. NCBI_GenBank
Match: gi|527200494|gb|EPS67911.1| (hypothetical protein M569_06860, partial [Genlisea aurea])

HSP 1 Score: 276.944 bits (707), Expect = 4.891e-81
Identity = 179/536 (33.40%), Postives = 270/536 (50.37%), Query Frame = 0

Query:  172 TLYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPSTALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAG---YTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFSFYGVDVMVTKDLQPKILECTFGPDCASALKLDA--------------DFYNKAFGIIFRGE 690
            +L ++V   +W Y  +YRL D  +     +VW+V DE GSA +H+  A  NFR+SPF+Y P  G L+ A+ YSL WP  ++  G+ECT DY FG    +              +R+ RL  WF  P   R  F   + + +    S        ++    PS+     F    R    VLRV+ D   V   LSR +     +  +AD++W   T +    ++  GL +   ++Q   + CLV KH L  T  +  G   WL  TYN+ET+ A  I +Y        R  EGA+              + L+ILKPWN+ R++D+ V D+L   ++L +T PK+  +Y+   A    RKFD+RY + +R++ PLEIF    + VR++N+PYSL      +++ HFTVM+Y G   +  TP    DF+E F++++  V W ++D ++ ++I+++FE A I      G     +S + YGVDVM+    QPK+LE T+ PDC  A K D               DFYN  FG +F  E
Sbjct:   58 SLIDRVLSAMWFYAMTYRLADE-EKIDETSVWYVMDELGSALRHSDEA--NFRVSPFLYMPD-GKLESAVSYSLLWPTKDVQRGDECTRDYLFGIGENK--------------QRSARLTAWFCTP---RDYFLREYRKHETKLRS-----EKNTRFPEIPSSTTCNLFEIHGR----VLRVFTDIPPVEECLSRPEFIITTDVKDADIIWT-STQVDEEMKKAAGLTVKQYINQFPFEACLVMKHHLAETVQKVHGVADWLQPTYNLETQLAQLIGDY------LSREMEGAD--------------NNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFRGRKFDLRYIVLIRSFNPLEIFLTDIFWVRLANNPYSLEKHTFFEYETHFTVMNYRGKLNHMNTP----DFVEGFQQEH-HVDWLDIDGRIRQMIRSVFEAAVI---THPGIHS-PKSRAMYGVDVMLDSHYQPKLLEVTYCPDCTRACKYDTQTATGERGGIVRGRDFYNIVFGCLFLNE 533          

BLAST of EWM30567.1 vs. NCBI_GenBank
Match: gi|1025110720|ref|XP_016461435.1| (PREDICTED: tubulin--tyrosine ligase-like protein 12 [Nicotiana tabacum])

HSP 1 Score: 283.493 bits (724), Expect = 1.388e-80
Identity = 183/535 (34.21%), Postives = 273/535 (51.03%), Query Frame = 0

Query:  173 LYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPS-TALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAG---YTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFSFYGVDVMVTKDLQPKILECTFGPDCASALKLDA-------------DFYNKAFGIIFRGE 690
            L ++V   +W Y+ +YRL D  +     +VW+V DEFGSA +H+     NFR+SPF+Y P  G+L  A+ YS+ WPI ++ EG+ECT DY FG           EEK     +R+ RL  WF  P   R  F   + + K    S     N    PV   S T    H   S       LRVY D   V  +L+R +     EP +AD++W     I    ++  GL  +  ++Q   + CLV KH L  T  +A G   WL  TYN+ET+    I ++ +R  E                      L  L+ILKPWN+ R++D+ +  +L   ++L +T PK+  +Y+   A    RKFD+RY + VR+  PLEIF    + VR++N+ Y+L   + ++++ HFTVM+Y G   +  TP    DF++ FE+++  V W ++ S++ ++I + FE AA++  E       ++S + YGVDVM+    QPK+LE T+ PDC  A+K D              DFYN  FG +F  E
Sbjct:  382 LEDRVINAMWLYLMTYRLADE-EKIDETSVWYVMDEFGSALRHSDKP--NFRVSPFLYMP-EGNLASAVSYSIIWPIDDVREGDECTRDYLFGIG---------EEK-----QRSARLTAWFHTP---RNYFIKEYEKYKNKLQS-----NKFVSPVQGSSVTNSLCHGDGS------ALRVYTDIPPVEEYLTRPEFVITTEPKDADIIWT-SMQIDEETKKAAGLNDEQYINQFPFEACLVMKHHLAETIQKAHGLVEWLQPTYNLETQLTQLIGDFHQREREK---------------------LDNLWILKPWNMARTIDTTITSNLSAIIRLMETGPKIGQKYIEHPALFKGRKFDLRYIVLVRSMNPLEIFLAEVFWVRLANNTYTLEKHSFDEYETHFTVMNYRGRLNHMNTP----DFVKEFEKEH-KVNWLDIHSRIRRMITSAFEAAAVVHPEM----HHSKSRAMYGVDVMLDCHFQPKLLEITYCPDCGRAVKYDTEAVVGGGETVKGKDFYNYIFGCLFLNE 853          

BLAST of EWM30567.1 vs. NCBI_GenBank
Match: gi|1111104744|ref|XP_019245868.1| (PREDICTED: tubulin--tyrosine ligase-like protein 12 isoform X1 [Nicotiana attenuata] >gi|1102147231|gb|OIT03544.1| hypothetical protein A4A49_11377 [Nicotiana attenuata])

HSP 1 Score: 283.493 bits (724), Expect = 1.867e-80
Identity = 183/535 (34.21%), Postives = 273/535 (51.03%), Query Frame = 0

Query:  173 LYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPS-TALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAG---YTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFSFYGVDVMVTKDLQPKILECTFGPDCASALKLDA-------------DFYNKAFGIIFRGE 690
            L ++V   +W Y+ +YRL D  +     +VW+V DE GSA +H+  +  NFR+SPF+Y P  G+L  A+ YS+ WPI ++ EG+ECT DY FG           EEK     +R+ RL  WF  P   R  F   + + K    S     N    PV   S T    H   S       LRVY D   V  +L+R +     EP +AD++W     I    ++  GL  +  ++Q   + CLV KH L  T  +A G   WL  TYN+ET+    I ++ +R  E                      L  L+ILKPWN+ R++D+ +  +L   ++L +T PK+  +Y+   A    RKFD+RY + VR+  PLEIF    + VR++N+ Y+L   + ++++ HFTVM+Y G   +  TP    DF++ FE+++  V W ++ S++  +IK+ FE AA++  E       ++S + YGVDVM+    QPK+LE T+ PDC  A+K D              DFYN  FG +F  E
Sbjct:  382 LEDRVINAMWLYLMTYRLADE-EKIDETSVWYVMDELGSALRHSDKS--NFRVSPFLYMPE-GNLASAVSYSILWPIDDVREGDECTRDYLFGIG---------EEK-----QRSARLTAWFHTP---RNYFIKEYEKYKNKLQS-----NKFVSPVQGSSVTNSLCHGDGS------ALRVYTDIPPVEEYLTRPEFVITTEPKDADIIWT-SMQIDEETKKAAGLNDEQYINQFPFEACLVMKHHLAETIKKAHGLVEWLQPTYNLETQLTQLIGDFHQREREK---------------------LDNLWILKPWNMARTIDTTITSNLSAIVRLMETGPKICQKYIEHPALFQGRKFDLRYIVLVRSMNPLEIFLAEVFWVRLANNTYTLEKHSFDEYETHFTVMNYRGRLNHMNTP----DFVKEFEKEH-EVNWLDIHSRIRSMIKSAFEAAAVVHPEM----HHSKSRAMYGVDVMLDCQFQPKLLEITYCPDCGRAVKYDTEAVVGGGETVKGKDFYNYIFGCLFLNE 853          

BLAST of EWM30567.1 vs. NCBI_GenBank
Match: gi|1025327002|ref|XP_016505294.1| (PREDICTED: tubulin--tyrosine ligase-like protein 12 [Nicotiana tabacum])

HSP 1 Score: 281.952 bits (720), Expect = 6.558e-80
Identity = 179/534 (33.52%), Postives = 270/534 (50.56%), Query Frame = 0

Query:  173 LYEQVCEQIWRYVSSYRLVDPGDPFKFKTVWFVKDEFGSAFQHAPPALVNFRISPFIYFPPTGSLKGAMGYSLAWPIANIAEGEECTHDYAFGCANVRDNSITQEEKFLRWMKRACRLAVWFELPLSVRAIFEAAHAERKQLFLSLAVSVNAASKPVADPSTALAAHFSRSWRHSPLVLRVYNDNGQVLRHLSRADVQRAAEPAEADVLWVYHTAITPHYRRRHGLGLDVVVSQTGGDECLVFKHLLPLTATRAPGSQAWLPDTYNMETETAAFIHEYSRRSDEAHRSGEGAEHPPHAQEHGPSTPLSPLFILKPWNLGRSMDSAVADSLPQALQLAKTCPKVASQYVYDIATVNDRKFDVRYRLFVRAWEPLEIFRWVHWGVRVSNHPYSLAAENINDFQAHFTVMDYAG---YTQTPVSETDFLEAFERDNGGVPWSEVDSKVEKIIKNLFETAAILILERTGREEVAQSFSFYGVDVMVTKDLQPKILECTFGPDCASALKLDA-------------DFYNKAFGIIFRGE 690
            L ++V   +W Y+ +YRL D  +     +VW+V DE GSA +H+     NFR+SPF+Y P  G+L  A+ YS+ WPI ++ EG+ECT DY FG           EEK     +R+ RL  WF  P   +  F   + + K    S     N    PV   S   +             LRVY D   V  +L+R +     EP +AD++W     I    ++  GL  +  ++Q   + CLV KH L  T  +A G   WL  TYN+ET+    I ++ +R  E                      L  L+ILKPWN+ R++D+ +  +L   ++L +T PK+  +Y+   A    RKFD+RY + VR+  PLEIF    + VR++N+ Y+L   + ++++ HFTVM+Y G   +  TP    DF++ FE+++  V W ++ S++  +IK+ FE AA++  E       ++S + YGVDVM+    QPK+LE T+ PDC  A+K D              DFYN  FG +F  E
Sbjct:  382 LEDRVINAMWLYLMTYRLADE-EKIDETSVWYVMDELGSALRHSDKP--NFRVSPFLYMP-EGNLASALSYSILWPIDDVREGDECTRDYLFGIG---------EEK-----QRSARLTAWFHTP---KNYFLKEYEKYKNKLQS-----NKFVSPVQGSSVTNSLRLGDG-----SALRVYTDIPPVEEYLTRPEFVITTEPKDADIIWT-SMQIDEERKKAAGLNDEQYINQFPFEACLVMKHHLAETIQKAHGLVEWLQPTYNLETQLTQLIGDFHQREREK---------------------LDNLWILKPWNMARTIDTTITSNLSAIIRLMETGPKIGQKYIEHPALFKGRKFDLRYIVLVRSMNPLEIFLAEVFWVRLANNTYTLEKHSFDEYETHFTVMNYRGRLNHMNTP----DFVKEFEKEH-EVNWLDIHSRIRSMIKSAFEAAAVVHPEM----HHSKSRAMYGVDVMLDCHFQPKLLEITYCPDCGRAVKYDTEALGGGGETVKGKDFYNYIFGCLFLNE 853