EWM22347.1, cds8315 (CDS) Nannochloropsis gaditana

BLAST of EWM22347.1 vs. NCBI_GenBank
Match: gi|585102847|gb|EWM22347.1| (trna pseudouridine synthase pus10 [Nannochloropsis gaditana])

HSP 1 Score: 1245.34 bits (3221), Expect = 0.000e+0
Identity = 611/611 (100.00%), Postives = 611/611 (100.00%), Query Frame = 0

Query:    1 MPVSPPPSLPLPDEDTWSLPAFSLLYPKINCIRCSLRLAGVCDAEILRAPERQLRATLCSAQGAEISHATCRMCPLCLGILQSSGDRELGSALLDAILAQIKTKKYVLPTGGDSPDMTQHKSTTDPVGEMVAEHSKYLLERKVPFILGVSLPGSVAIRDRAALEWVACEQQTGRHQDPGRRLDVKEILKAVLESCLPKHYPCLYAASTPSPGALRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMPPSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIPGREEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGIVMEGEEGEREEGKEQEEEAGGGVKGAEINGIVV 611
            MPVSPPPSLPLPDEDTWSLPAFSLLYPKINCIRCSLRLAGVCDAEILRAPERQLRATLCSAQGAEISHATCRMCPLCLGILQSSGDRELGSALLDAILAQIKTKKYVLPTGGDSPDMTQHKSTTDPVGEMVAEHSKYLLERKVPFILGVSLPGSVAIRDRAALEWVACEQQTGRHQDPGRRLDVKEILKAVLESCLPKHYPCLYAASTPSPGALRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMPPSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIPGREEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGIVMEGEEGEREEGKEQEEEAGGGVKGAEINGIVV
Sbjct:    1 MPVSPPPSLPLPDEDTWSLPAFSLLYPKINCIRCSLRLAGVCDAEILRAPERQLRATLCSAQGAEISHATCRMCPLCLGILQSSGDRELGSALLDAILAQIKTKKYVLPTGGDSPDMTQHKSTTDPVGEMVAEHSKYLLERKVPFILGVSLPGSVAIRDRAALEWVACEQQTGRHQDPGRRLDVKEILKAVLESCLPKHYPCLYAASTPSPGALRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMPPSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIPGREEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGIVMEGEEGEREEGKEQEEEAGGGVKGAEINGIVV 611          

BLAST of EWM22347.1 vs. NCBI_GenBank
Match: gi|1173942599|gb|OQR91567.1| (tRNA pseudouridine synthase [Achlya hypogyna])

HSP 1 Score: 218.009 bits (554), Expect = 2.660e-60
Identity = 180/557 (32.32%), Postives = 251/557 (45.06%), Query Frame = 0

Query:   31 CIRCSLRLAGVCDAEILRAPERQLRATLCS--AQGAEISHATCRMCPLCLGILQSSGDRELGSALLDAILAQIKTKKYVLPTGGDSPDMTQHKSTTDPVGEMVAEHSKYLLERKVPFILGVSLPGSVAIRDRAALEWVACEQQTGRHQDPGRRLDVKEILKAVLESCLPKHYPCLYAASTPSPGALRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMPPSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIPGREEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGIVMEGEEG 585
            CIRC LR AG+CD  +   P  +L AT  +  AQ A+    +  +C  CL +     D  L  A  +A+ A +    Y  P+                                  F++   +P SV IR  A L +V         Q     +D+KE+LK  +    P     L  A+  +   + V + F H  ++         +LLP          R      +  + ++     V+R L          SGL    A    PP+        A+++PA   V V R  +++AGRYLK  R + QT WV++G  R G +SVEE + + +     A S     +GRED+DVRMLG GRPF+L + D +      E     A +       +   V V  L  + K    A+ +GA  K K Y C VW  G +  A +AT L G  +L VAQKTP+RVLHRR LLTRPKVIH+ R                  E+    Y  L L TSAGTY+KEFVHGD+GRT P+V  LL G +AD+L LDV  ++ +G+ G
Sbjct:   29 CIRCCLRFAGLCDEALYLLPSSELEATWAAYGAQHAQAVTPSAPICTCCLNVF----DVLLAPAGREAVTATLAEAGYTTPS----------------------------------FVIATKIPSSVLIRQYAMLHFV---------QAKLTPVDLKEVLKWCIT---PIFAASLGGATYNAASDVSVHVHFTHPLSESE------AMLLPT--------LRDTIMQNKKRKLDIDGFGAVSRAL----------SGLRSLPAAIPSPPT--------AVQSPATLVVTVERSPVYVAGRYLKYQRGLSQTPWVLDG-ERMGDSSVEEAIGDVIAPEFRAKSYKFHTAGREDVDVRMLGNGRPFILELLDAKVATLSPEAF---ATVQNRVNAANLDVVEVRELTGATKEGFAALQSGADSKRKTYCCVVWTSGVLTPARVAT-LNGIVDLKVAQKTPVRVLHRRTLLTRPKVIHDARV-----------------EVLNDHYMLLRLTTSAGTYVKEFVHGDLGRTFPNVASLL-GCDADILQLDVENLI-DGQPG 479          

BLAST of EWM22347.1 vs. NCBI_GenBank
Match: gi|514803213|ref|XP_004976495.1| (PREDICTED: putative tRNA pseudouridine synthase Pus10 [Setaria italica])

HSP 1 Score: 216.468 bits (550), Expect = 3.283e-59
Identity = 178/578 (30.80%), Postives = 265/578 (45.85%), Query Frame = 0

Query:   31 CIRCSLRLAGVCDA-------------EILRAPERQLRATLCSAQGAEISHATCRMCPLCLGILQSSGDRELGSALLDAILAQIKTKKYVLPTGGDSPDMTQHKSTTDPVGEMVAEHSKYLLERKVPFILGVSLPGSVAIRDRAALEWVACEQQTGRHQ------DPGRRLDVKEILKAVLESCLPKHYPCLYAASTPSPGALRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNL-----MPPSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIPGREEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEG---SKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGIVME 581
            C+RC LRL G   +               L   +  ++   CS   A+ S+     C +CLG+L  +  RE G          ++T       GG   D     S +  + ++V + S YL++    F L +SLP  +A  +RA   ++  +Q+ G          P + + VKE L+ ++   L K        +     + R+ L++ H  A      Q +  LLPN  G+   +T  R   + SN+ +  N+T      Q +  D  +   L     + L     +PP          +  P    +   R  I++ GRYLKLSR + Q+ W+++   R G+ASVEE++ E +       S     +GRED+DVRMLG+GRPFL+ + + R IP   EV  +E  IN +        VRV  L+L        M  G +EK K Y+  +W      R L    L     +K++ + QKTPIRVLHRR+ L R ++IH M    VK                   Y+ LHL T AGTYIKEFVHGD+GRT PS+G +  G  A++L LDVT + M+
Sbjct:   33 CVRCILRLLGAYSSACSFASLTASVLHSFLEEHDNSIKGGSCSCLSADDSY-----CSVCLGVLLPAWHREEG----------VETSH-----GGFHID-----SISSMISQVVQKES-YLVDE---FSLEISLPPVIAANERAVRLYM--KQKFGNENWFKDKMFPQQIMSVKEALRLLMVPSLEKQ-----MNAKHDNCSFRIRLTYTHDDAS-----QKLHSLLPNEHGRK-RKTDSRNGSDTSNEAHKRNSTD-GNNKQISESDSFIYKTLEGIQDQELCNLIQLPPE--------KVSKPCHLVISCMRSPIYIGGRYLKLSRNVSQSCWIIDD-ERMGEASVEEIIGENIRVICRGDSCKFHAAGREDIDVRMLGSGRPFLVELLNVRSIPSEIEVQQIEDRINNS----EKKYVRVRNLKLVGNEIWTMMREGESEKQKQYTALIWTS----RELAENDLHNISVTKDMEIVQKTPIRVLHRRSPLERKRIIHWMEIEKVK---------------GSSNYYLLHLCTQAGTYIKEFVHGDLGRTHPSIGAIF-GCRAEILQLDVTDVKMD 534          

BLAST of EWM22347.1 vs. NCBI_GenBank
Match: gi|673025686|ref|XP_008866804.1| (tRNA pseudouridine(54/55) synthase, variant 2 [Aphanomyces invadans] >gi|574478368|gb|ETW05364.1| tRNA pseudouridine(54/55) synthase, variant 2 [Aphanomyces invadans])

HSP 1 Score: 214.157 bits (544), Expect = 5.977e-59
Identity = 175/559 (31.31%), Postives = 251/559 (44.90%), Query Frame = 0

Query:   31 CIRCSLRLAGVCDAEILRAPERQLRAT---LCSAQGAEISHATCRM---CPLCLGILQSSGDRELGSALLDAILAQIKTKKYVLPTGGDSPDMTQHKSTTDPVGEMVAEHSKYLLERKVPFILGVSLPGSVAIRDRAALEWVACEQQTGRHQDPGRRLDVKEILKAVLESCLPKHYPCLYAASTPSPGALRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMP--PSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPR--RIPGREEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGIV 579
            C+RC LR A V D E+      +L AT     +AQG  +  A   +   C  CL + + +    LG++    ++A+ K   Y   T                                  F++ + +P +  IR  +    V         + P   +D+KE+LK  L   L      L  A+  +   + + L F                                 +HE S QE +   T+   ++Q       + +  AV  A   M   PST  P      +TP + +V++ R   ++AGRYLK  R + QT WV++G  R G++SVEE + + VL    A S     +GRED+DVRMLG GRPF+L + D +   +P       ++A +N+T    +   V + G +L+DK     + AGA  K K Y C VWV  ++    +AT L    +L VAQKTP+RVLHRR LLTRPKVIH    T +                    Y  L L TSAGTY+KEFVHGD+GRT P+V  LL G +AD+L LDV  +V
Sbjct:   28 CVRCILRYAAVTDHELYSLDSAELSATWDTFSAAQGQPVPDAPAAIAGVCTCCLDVFEGA----LGASNRADLVAKAKDCGYATTT----------------------------------FMIAIQIPSATLIRQHSLSHHV---------KFPSTPIDLKEVLKWCLTPILAT---ALNNATYVATSDVSIHLHF---------------------------------QHELSEQEAMQVPTIRDTIMQNKRRKLEIDAFGAVTRALQSMHDFPST-MPCPPTVAKTPCSLTVKMERAPTYVAGRYLKYQRGLSQTPWVLDG-ERLGESSVEEAIGDIVLPHFHAKSYKFHTAGREDVDVRMLGNGRPFILELIDAKVAALPSPAHYERIQAEVNET----NADRVEIRGFQLTDKKRFSVLQAGADSKRKTYCCVVWVAEKLTPDRVAT-LNSISDLAVAQKTPVRVLHRRTLLTRPKVIHSANVTVLN-----------------DHYMLLRLTTSAGTYVKEFVHGDLGRTLPNVSTLL-GCDADILQLDVEDLV 478          

BLAST of EWM22347.1 vs. NCBI_GenBank
Match: gi|971541324|ref|XP_015161917.1| (PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2 [Solanum tuberosum])

HSP 1 Score: 214.157 bits (544), Expect = 3.776e-58
Identity = 184/593 (31.03%), Postives = 262/593 (44.18%), Query Frame = 0

Query:   14 EDTWSLPAFSLLYPKINCIRCSLRLAGV---------------CDAEILRAPERQLRATLCSAQGAEISHATCRMCPLCLGILQSSGDRELGSALLDAILAQIKTKKYVLPTGGDSPDMTQHKSTTDPVGEMVAEHSKYLLERKVPFILGVSLPGSVAIRDRAALEWVACEQQTGRHQ------DPGRRLDVKEILKAVLESCLPKHYPCLYAASTPSPGALRVDLSFWHAGA----DEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMPPSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIPGREEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGIVME 581
            +D WS+ A         C+RC  RL G+               CD  IL    R+           E S  T   C +CLGILQ     + G+ L          KKY                + D    +++E  K   ++   F L VSLP SV   D+A   W+  + +  RH+         + +  K+ILK ++ + L +           S  + RV L++ H+ A    D  D + G +      TG           H ES+  ++ +   + +V    + D +           NLM            +  P   ++   R + ++ GRYLK SR + QT WV++   R G+ASVEE++   +L      +     +GRED+DVRMLGTGRPFL+ + + R++P    +  +E  IN          VRV  LR         M  G  EK K Y+  VW+   V    L T +   KEL VAQKTPIRVLHRR+ L R K+IH M+    + V G  +            YF LHL T AGTYIKEFVHGD GRT+PS+G +L G   ++L LDVT + M+
Sbjct:   44 KDLWSIGA---------CVRCIFRLFGLHELMCSHPSLSISKWCD--ILEDVMRKKDGEFNEQSHQERSRET-NFCKICLGILQFLYYDDTGTLL----------KKY----------------SADDFAVVISELVKQQYQQIDSFSLEVSLPSSVTENDQAV--WLYMKNKF-RHELWFQEKAECQFISTKDILKLLVINPLQR-----LLGVKSSQSSFRVRLTYSHSDALTRDDVQDGNGGNKRRKTGFTGDLKTTGESVTPHVESSDNHIVDGDGLTKVCPKESQDLS---------CNNLMAQ-------LDKVGKPCCLTIHCCRSSTYIGGRYLKYSRNVSQTRWVIDD-ERMGEASVEEIIGGAILPIFQGDNYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEALIKEIERKINSL----ESHYVRVKNLRAVGIQGWDLMREGEAEKQKQYAALVWISRPVSDEDLQT-ISSLKELKVAQKTPIRVLHRRSPLEREKIIHWMQ---AERVSGSSQ------------YFLLHLCTQAGTYIKEFVHGDFGRTQPSIGSIL-GCRTEILQLDVTDVKMD 552          

BLAST of EWM22347.1 vs. NCBI_GenBank
Match: gi|699669664|ref|XP_009884377.1| (PREDICTED: putative tRNA pseudouridine synthase Pus10 [Charadrius vociferus])

HSP 1 Score: 213.001 bits (541), Expect = 4.724e-58
Identity = 174/575 (30.26%), Postives = 260/575 (45.22%), Query Frame = 0

Query:   20 PAFSLLYPKINCIRCSLRLAGVCDAEILRAPERQL----RATLCSAQGAEISHATCRMCPLCLGILQSSGDRELGSALLDAILAQIKT--------KKYVLPTGGDSPDMTQHKSTTDPVGEMVAEHSKYLLERKVPFILGVSLPGSVAIRDRAALEWVACEQQTGRHQDPGRRLDV---KEILKAVLESCLPKHYPCLYAASTPSPGALRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMPPSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIPGR-EEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGI 578
            P   LL     C RC LR   V    + R P + L    +  L + Q  E +  +  + P C  I     +++      +  L QI +        +  V+        + Q     D V + V +       +   F+  VSLP  +++R+RAA  W+  +Q+ G+      + D+   KE  K ++   L +    L+  S          + F H   DE  +       L  A    F   +        N++++     V + L+    D          F ++   P +       ALE      +Q    A+F+AGRY K SR +PQT W+++G  RK ++SVEE+++E ++    A S     SGRED+DVR LG GRPF + + +PRRI    EE+ GL+  IN     NS   ++V  L+L  +  +  M  G  EK K YS  +W    + +  +A  L+  KEL + QKTP+RVLHRR L  R ++IH M+                  E     +F LHL+T AGTYIKEFVHGD GRT+PS+G LL    AD+L LDV  +
Sbjct:   10 PIVQLLLNTGTCPRCILRFCCVGSQTLYRCPYKDLMKDLKEFLKNNQEKEDTVCSDVVDPPCKRIRLEHTEKQPDDLNHNGALQQIPSVINENTAVENSVIEVCNVCLGILQEFCEIDFVKK-VCQKVNSADYQFTSFVFSVSLPPQLSVRERAA--WLLVKQEMGKLGLSLAKDDIVQLKEAYKWIIHPQLSEELGSLFEVS----------VVFAHPETDEECH------FLATACPDCFKPAK--------NKQSVFTRMAVIKALEKIKEDD---------FLKHFPCPPSSPKNLCVALE------IQCNNGAVFVAGRYNKYSRNLPQTPWIIDG-ERKLESSVEELISEHLMAKFKADSFNFSSSGREDVDVRTLGNGRPFAIELVNPRRIHFTAEEMKGLQQTIN-----NSSDKIQVRDLQLVTREAIGRMKEGEEEKTKTYSALIWTDKAIRKEDIAF-LDDIKELKLDQKTPLRVLHRRPLAVRCRIIHTMK-----------------SEYIDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPSIGSLLNRT-ADILELDVESV 517          

BLAST of EWM22347.1 vs. NCBI_GenBank
Match: gi|543264914|ref|XP_005422688.1| (PREDICTED: putative tRNA pseudouridine synthase Pus10 [Geospiza fortis])

HSP 1 Score: 213.001 bits (541), Expect = 5.157e-58
Identity = 155/438 (35.39%), Postives = 217/438 (49.54%), Query Frame = 0

Query:  145 FILGVSLPGSVAIRDRAALEWVACEQQTGRHQDPGRRLDVKEILKAVLESCLPKHYPCLYAASTPSPGA--LRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMPPSTHSPTAYA-ALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIP-GREEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGI 578
            F+  VSLP  +++R+RAA  W+  +QQ      P  + D+ ++ +A      P+       A  PS G     V + F H   DE  +       L  A    F   +        N++++     V + L+        R     F      PPS  SP     ALE      +Q    A+F+AGRY K SR +PQT W+++ G RK ++SVEE+++E ++    A S     SGRED+DVR LG GRPF + + +PRRI    EE+  L+ AIN     +S   ++V  L+L  +  +  M  G  EK K YS  +W    + R  +A  L+  KEL + QKTP+RVLHRR L  R ++IH MR                  E     +F LHLRT AGTYIKEFVHGD GRT+P++G LL    AD+L LDV  +
Sbjct:  150 FVFSVSLPPQLSVRERAA--WLGVKQQMRNLGLPLAKDDIVQLKEAYKWIIHPQ------LAEDPSDGKSLFEVSVVFAHPETDEECH------FLATACPDCFKPAK--------NKQSVFTRMAVIKALE--------RIKEEDFLKHFPCPPS--SPKNLCDALE------IQCNNGAVFVAGRYNKYSRNLPQTPWIID-GERKLESSVEELISEHLMAEFKADSFNFSSSGREDVDVRTLGNGRPFAMELVNPRRIHFSAEEMKRLQQAIN-----DSSDKIQVRDLQLVTREAIGRMKEGEEEKTKTYSALIWTDKAIQREDIAF-LDDIKELRLEQKTPLRVLHRRPLAVRCRLIHSMR-----------------SEYIDQHHFRLHLRTQAGTYIKEFVHGDFGRTKPNIGSLLNRT-ADILELDVESV 524          

BLAST of EWM22347.1 vs. NCBI_GenBank
Match: gi|960952931|ref|XP_014821859.1| (PREDICTED: putative tRNA pseudouridine synthase Pus10 [Calidris pugnax] >gi|960952933|ref|XP_014821860.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 [Calidris pugnax] >gi|960952935|ref|XP_014821861.1| PREDICTED: putative tRNA pseudouridine synthase Pus10 [Calidris pugnax])

HSP 1 Score: 212.616 bits (540), Expect = 7.309e-58
Identity = 151/440 (34.32%), Postives = 217/440 (49.32%), Query Frame = 0

Query:  145 FILGVSLPGSVAIRDRAALEWVACEQQTGRHQDPGRRLDV---KEILKAVLESCLPKHYPCLYAASTPSPGA--LRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMPPSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIPGR-EEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGI 578
            F+  VSLP  +++R+RAA  W+  +Q+TG+      + D+   KE  K ++   L +          P+ G     V + F H   DE          L  A    F   +        N++++     V + L+    +         F      PPS  SP   +      A  +Q    A+F+AGRY K SR +PQT W+++G  RK ++SVEE+++E ++    A S     SGRED+DVR LG GRPF + + +PRRI    EE+ GL+  IN     NS   ++V  L+L  +  +  M  G  EK K YS  +W    + R  + T L+  KEL + QKTP+RVLHRR L  R ++IH M+                  E     +F LHL+T AGTYIKEFVHGD GRT+P++G LL    AD+L LDV  +
Sbjct:  146 FVFSVSLPPQLSVRERAA--WLLVKQETGKLGLSLAKEDIVQLKEAYKWIIHPQLSEEL------GVPADGKSLFEVSVVFAHPETDEECR------FLATACPDCFKPAK--------NKQSVFTRMAVIKALEKIKEED--------FLKHFPCPPS--SPKNLSV-----ALEIQCNNGAVFVAGRYNKYSRNLPQTPWIIDG-ERKLESSVEELISEHLMAEFKADSFNFSSSGREDVDVRTLGNGRPFAIELVNPRRIHFTAEEMKGLQQTIN-----NSSDKIQVRDLQLVTREAIGRMKEGEEEKTKTYSALIWTDKAIQREDI-TFLDDIKELKLDQKTPLRVLHRRPLAVRCRIIHTMK-----------------SEYIDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLLNRT-ADILELDVESV 523          

BLAST of EWM22347.1 vs. NCBI_GenBank
Match: gi|697457786|gb|KGL94142.1| (Putative tRNA pseudouridine synthase Pus10, partial [Charadrius vociferus])

HSP 1 Score: 212.231 bits (539), Expect = 8.450e-58
Identity = 175/575 (30.43%), Postives = 260/575 (45.22%), Query Frame = 0

Query:   20 PAFSLLYPKINCIRCSLRLAGVCDAEILRAPERQL----RATLCSAQGAEISHATCRMCPLCLGILQSSGDRELGSALLDAILAQIKT--------KKYVLPTGGDSPDMTQHKSTTDPVGEMVAEHSKYLLERKVPFILGVSLPGSVAIRDRAALEWVACEQQTGRHQDPGRRLDV---KEILKAVLESCLPKHYPCLYAASTPSPGALRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMPPSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIPGR-EEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGI 578
            P   LL     C RC LR   V    + R P + L    +  L + Q  E +  +  + P C  I     +++      +  L QI +        +  V+        + Q     D V + V +       +   F+  VSLP  +++R+RAA  W+  +Q+ G+      + D+   KE  K ++       +P L      S     V + F H   DE  +       L  A    F   +        N++++     V + L+    D          F ++   P +       ALE      +Q    A+F+AGRY K SR +PQT W+++G  RK ++SVEE+++E ++    A S     SGRED+DVR LG GRPF + + +PRRI    EE+ GL+  IN     NS   ++V  L+L  +  +  M  G  EK K YS  +W    + +  +A  L+  KEL + QKTP+RVLHRR L  R ++IH M+                  E     +F LHL+T AGTYIKEFVHGD GRT+PS+G LL    AD+L LDV  +
Sbjct:   10 PIVQLLLNTGTCPRCILRFCCVGSQTLYRCPYKDLMKDLKEFLKNNQEKEDTVCSDVVDPPCKRIRLEHTEKQPDDLNHNGALQQIPSVINENTAVENSVIEVCNVCLGILQEFCEIDFVKK-VCQKVNSADYQFTSFVFSVSLPPQLSVRERAA--WLLVKQEMGKLGLSLAKDDIVQLKEAYKWII-------HPQL------SEDLFEVSVVFAHPETDEECH------FLATACPDCFKPAK--------NKQSVFTRMAVIKALEKIKEDD---------FLKHFPCPPSSPKNLCVALE------IQCNNGAVFVAGRYNKYSRNLPQTPWIIDG-ERKLESSVEELISEHLMAKFKADSFNFSSSGREDVDVRTLGNGRPFAIELVNPRRIHFTAEEMKGLQQTIN-----NSSDKIQVRDLQLVTREAIGRMKEGEEEKTKTYSALIWTDKAIRKEDIAF-LDDIKELKLDQKTPLRVLHRRPLAVRCRIIHTMK-----------------SEYIDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPSIGSLLNRT-ADILELDVESV 514          

BLAST of EWM22347.1 vs. NCBI_GenBank
Match: gi|698412341|ref|XP_009811615.1| (PREDICTED: LOW QUALITY PROTEIN: putative tRNA pseudouridine synthase Pus10 [Gavia stellata])

HSP 1 Score: 212.616 bits (540), Expect = 8.982e-58
Identity = 147/435 (33.79%), Postives = 215/435 (49.43%), Query Frame = 0

Query:  145 FILGVSLPGSVAIRDRAALEWVACEQQTGRHQDPGRRLDVKEILKAVLESCLPKHYPCLYAASTPSPGALRVDLSFWHAGADEADYHQGVRILLPNATGKAFNRTRKRRRHEESNQENLCNATVVARVLQAATGDPNVRSGLAVFFARNLMPPSTHSPTAYAALETPAASSVQVTREAIFLAGRYLKLSREMPQTLWVVEGGRRKGKASVEEVVAEPVLDWTSATSAVLIGSGREDMDVRMLGTGRPFLLRVNDPRRIPGR-EEVAGLEAAINQTTGLNSGGDVRVHGLRLSDKHEVKAMHAGATEKVKMYSCRVWVGGRVDRALLATSLEGSKELVVAQKTPIRVLHRRALLTRPKVIHEMRCTDVKVVEGGEEGGEEGGEMAVGTYFTLHLRTSAGTYIKEFVHGDMGRTRPSVGDLLGGLEADLLALDVTGI 578
            F+  VSLP  +++R+RAA  W+  +Q+TG+      + D+ ++ +A      P+                 V + F H   DE  +       L  A    F   +        N++++     V + L+    +         F      PPS  SP     +    A  +Q    A+F+AGRY K SR +PQT W+++G  RK ++SVEE+++E ++    A S     SGRED+DVR LG GRPF + + +PRRI    EE+ GL+  IN     NS   ++V  L+L  +  +  M  G  EK K YS  +W    + +  +A  L+G KEL + QKTP+RVLHRR L  R ++IH M+                  E     +F LHL+T AGTYIKEFVHGD GRT+P++G LL    AD+L LDV  +
Sbjct:  146 FVFSVSLPPQLSVRERAA--WLLIKQETGKLGLSLAKDDIVQLKEAYKWIIHPQLSEEXXXXXWKGVSLFEVSVVFAHPETDEECH------FLATACPDCFKPAK--------NKQSVFTRMAVIKALEKIKEED--------FLKHFPCPPS--SP-----MNLCVALEIQCNNGAVFVAGRYNKYSRNLPQTPWIIDG-ERKLESSVEELISEHLMAEFKADSFNFSSSGREDVDVRTLGNGRPFAIELVNPRRIHFTAEEMKGLQQTIN-----NSSDKIQVRDLQLVTREAIGRMKEGEEEKTKTYSALIWTDKAIQKEDIAF-LDGIKELKLDQKTPLRVLHRRPLAVRCRIIHTMQ-----------------SEYIDEHHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLLNRT-ADILELDVESV 524