EWM29829.1, cds823 (CDS) Nannochloropsis gaditana

Overview
NameEWM29829.1
Unique Namecds823
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002456.1:504460..504714 -
Alignment locationCM002456.1:503479..504081 -

Link to JBrowse

Properties
Property NameValue
Protein idEWM29829.1
Producthomocysteine s-methyltransferase
Orig transcript idgnl|cribi|Naga_100017g12.327.mrna
GbkeyCDS
Noteencoded by transcript variant 1; alternatively spliced
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002456.1supercontigCM002456.1:504460..504714 -
CM002456.1supercontigCM002456.1:503479..504081 -
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Molecular Function
TermDefinition
GO:0016740transferase activity
GO:0005488binding
GO:0008898S-adenosylmethionine-homocysteine S-methyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR003726S_MeTrfase
Homology
BLAST of EWM29829.1 vs. NCBI_GenBank
Match: gi|585112410|gb|EWM29829.1| (homocysteine s-methyltransferase [Nannochloropsis gaditana])

HSP 1 Score: 572.007 bits (1473), Expect = 0.000e+0
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0
Query:    1 MSSAGVRVTRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYGLTVEELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVWQDKRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACLLAK 285
            MSSAGVRVTRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYGLTVEELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVWQDKRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACLLAK
Sbjct:    1 MSSAGVRVTRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYGLTVEELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVWQDKRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACLLAK 285          
BLAST of EWM29829.1 vs. NCBI_GenBank
Match: gi|585112411|gb|EWM29830.1| (homocysteine s-methyltransferase [Nannochloropsis gaditana])

HSP 1 Score: 412.535 bits (1059), Expect = 6.074e-140
Identity = 200/201 (99.50%), Postives = 201/201 (100.00%), Query Frame = 0
Query:   85 ELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVWQDKRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACLLAK 285
            +LMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVWQDKRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACLLAK
Sbjct:  293 QLMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVWQDKRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACLLAK 493          

HSP 2 Score: 174.481 bits (441), Expect = 1.886e-47
Identity = 96/128 (75.00%), Postives = 102/128 (79.69%), Query Frame = 0
Query:    1 MSSAGVRVTRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYGLTVEELMDWHRPRLAIL--GETDADLL----AFETIPCQAEVVAILQL 122
            MSSAGVRVTRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYGLTVEE+     P L +L    T +  +     F + PC    V +LQL
Sbjct:   83 MSSAGVRVTRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYGLTVEEVSREIVPALDVLLPSRTSSPCMRPHAPFCSSPCMRPHVPLLQL 210          
BLAST of EWM29829.1 vs. NCBI_GenBank
Match: gi|553189163|ref|XP_005854945.1| (homocysteine S-methyltransferase, partial [Nannochloropsis gaditana CCMP526] >gi|422294116|gb|EKU21416.1| homocysteine S-methyltransferase, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 297.745 bits (761), Expect = 1.208e-99
Identity = 146/147 (99.32%), Postives = 147/147 (100.00%), Query Frame = 0
Query:   85 ELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVW 231
            +LMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVW
Sbjct:    9 QLMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVW 155          
BLAST of EWM29829.1 vs. NCBI_GenBank
Match: gi|1082655086|gb|OFZ80118.1| (homocysteine S-methyltransferase [Bdellovibrionales bacterium RIFOXYD1_FULL_53_11])

HSP 1 Score: 196.438 bits (498), Expect = 1.128e-57
Identity = 121/279 (43.37%), Postives = 159/279 (56.99%), Query Frame = 0
Query:   12 QAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYGLTVEELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKG-SERILIAYPNSGEVWQDKR--WL-PGTG-VCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACLLAK 285
            +A  LIRK V +A+EARD FW           +   AG   R++PL+AAS+G YGA  ADGSEYRG YGL+V EL D+HR RLA L +T AD+LAFET+PC  E  A ++LL EG  +          P WI+ +C+D   +  G     C+ +V     GS PQ+V +GVNC AP+HVE                  +I AA+  +++ +I YPNS E+W  +R  W+ P  G V P     AD A +W  AGAR++GGCCRT    I+ALR  LL K
Sbjct:   83 EAASLIRKSVEIAVEARDAFWLV---------QDSRAG---REKPLIAASIGPYGAFLADGSEYRGFYGLSVRELADFHRDRLAALVQTRADMLAFETVPCPEEAEAYVRLLEEGEAK----------PAWISFSCKDGLSVCQGVPFERCIDIV----AGS-PQIVAIGVNCTAPEHVE-----------------SLIRAARSRTDKAIIVYPNSSEIWDARRRTWVSPAHGPVKP-----ADFAPQWFEAGARIIGGCCRTGIADIRALRTRLLQK 312          
BLAST of EWM29829.1 vs. NCBI_GenBank
Match: gi|504246229|ref|WP_014433331.1| (homocysteine S-methyltransferase [Caldilinea aerophila] >gi|381383279|dbj|BAM00096.1| homocysteine S-methyltransferase [Caldilinea aerophila DSM 14535 = NBRC 104270])

HSP 1 Score: 196.052 bits (497), Expect = 2.201e-57
Identity = 116/277 (41.88%), Postives = 154/277 (55.60%), Query Frame = 0
Query:    8 VTRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYGLTVEELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVW--QDKRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACL 282
            +  AQ  +L R  V LA EA +E+ A            E   G  R  PL+AAS+G YGA  ADGSEYRG YGL+VE L+ WHRPR++ L ET+ADL A ETIPC AE  A+++LL E  +           P W++ +C+D   L+SGE     V+L    +     Q+V +GVNC AP+ VE  L + R                  +++ L+ YPNSGE W  + + W+ GTGV     DFA+ AR+W  AGAR++GGCCRTTP  I A+   L
Sbjct:   81 LNEAQVRNLFRLSVQLAAEAIEEYLA------------ETQAGPARLPPLIAASIGPYGAYLADGSEYRGDYGLSVEALIAWHRPRVSALAETEADLFACETIPCLAEAEALIRLLEEYPDM----------PAWLSFSCRDGESLSSGEPFAEAVRLANRSE-----QIVAVGVNCTAPRFVESLLQIAR----------------PLTDKPLLCYPNSGEAWDAEARCWVEGTGVT----DFAEPARRWYAAGARLIGGCCRTTPADIAAMAQAL 310          
BLAST of EWM29829.1 vs. NCBI_GenBank
Match: gi|1194436361|gb|OUC08407.1| (homocysteine methyltransferase [Litorilinea aerophila])

HSP 1 Score: 195.282 bits (495), Expect = 2.883e-57
Identity = 118/273 (43.22%), Postives = 154/273 (56.41%), Query Frame = 0
Query:    8 VTRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYGLTVEELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVWQDKR--WLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKAL 278
            ++R QAE+L+   V LA EARD FWA            + A    R RPLVAAS+G YGA  ADGSEYRG YGL+VE LMD+HRPR+A+L  + ADLLA ETIPCQAE  A+++LL E  +             W++ +C D  H+  GE    CV+L    +     QV+ +G+NC  P+HVE  L                  A   ++R L+AYPNSGE W  +   W+ G+G      DF   A +W  AGAR++GGCCRTTP  I+A+
Sbjct:   82 LSREQAEELLLLSVRLAQEARDLFWA------------DPANRQGRIRPLVAASIGPYGAYLADGSEYRGDYGLSVEALMDFHRPRMAVLAASGADLLACETIPCQAEGEALVRLLAEFPQAVA----------WLSFSCCDDLHVCHGEPFARCVRLASRSE-----QVIAVGLNCTPPRHVEALLR----------------HAVGVTDRPLVAYPNSGETWDAEHHCWVAGSG----ETDFRTPALRWYAAGARLIGGCCRTTPADIQAM 307          
BLAST of EWM29829.1 vs. NCBI_GenBank
Match: gi|944227510|gb|KQK91914.1| (hypothetical protein SETIT_036632mg [Setaria italica])

HSP 1 Score: 193.356 bits (490), Expect = 5.193e-57
Identity = 114/279 (40.86%), Postives = 151/279 (54.12%), Query Frame = 0
Query:    8 VTRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYG--LTVEELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVWQD--KRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACL 282
            +T  +AEDL++  V LA+EARDEFW          + T         R LVAAS+G YGA  ADGSEY G+YG  +T E+L D+HR RL +L     DL+AFE IP + E  A+++LL E   Q          P WI  +  D  HL SGES   C+Q++ A +     +V  +GVNC  PQ +EG +   R                K +++ +  YPNSGEVW    KRWLP    C   + F  LA++W  AGA ++GGCCRTTP TI+A+   L
Sbjct:   32 MTLDEAEDLLQTSVKLALEARDEFW----------KSTLRKSKPIYNRALVAASIGSYGAYLADGSEYSGSYGADITTEKLKDFHRRRLQVLAGAGPDLIAFEAIPNKMEAQALVELLEEENIQ---------VPSWICFSSVDGKHLCSGESFADCLQILNASE-----KVAVVGVNCTPPQFIEGIICEFR----------------KQTKKAIAVYPNSGEVWDGIAKRWLPAE--CLGHKSFDALAKRWHEAGASLIGGCCRTTPSTIRAVSKIL 268          
BLAST of EWM29829.1 vs. NCBI_GenBank
Match: gi|1174567826|gb|OQU92985.1| (hypothetical protein SORBI_3001G453100 [Sorghum bicolor])

HSP 1 Score: 192.971 bits (489), Expect = 7.588e-57
Identity = 116/278 (41.73%), Postives = 153/278 (55.04%), Query Frame = 0
Query:   12 QAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYG--LTVEELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVW--QDKRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACLLAK 285
            +AEDL+R  V LA+EARDEFW +  R+                R LVAASVG YGA  ADGSEY G+YG  +T E+L D+HR RL +L     DL+AFE IP + E  A+++LL E + Q          P WI  +  D  +L SGES   C+++   LDT  +  VVG  VNC  PQ +EG +   +                K +++ +  YPNSGEVW  + KRWLP    C   + F  LA++W  AGA ++GGCCRTTP TI+A+   L  K
Sbjct:   32 EAEDLLRTSVKLAVEARDEFWKSALRK----------AKPIYNRALVAASVGSYGAYLADGSEYSGSYGADITAEKLKDFHRRRLQVLASAGPDLIAFEAIPNKMEAQALVELLEEEKVQ---------VPSWICFSSVDGKNLCSGESFADCLKI---LDTSDKVAVVG--VNCTPPQFIEGIICEFK----------------KQTKKAIAVYPNSGEVWDGRAKRWLPVE--CLGHKSFDALAKRWQEAGASLIGGCCRTTPSTIRAVSKILKGK 267          
BLAST of EWM29829.1 vs. NCBI_GenBank
Match: gi|514820963|ref|XP_004985165.1| (PREDICTED: homocysteine S-methyltransferase 1 [Setaria italica] >gi|514820965|ref|XP_004985166.1| PREDICTED: homocysteine S-methyltransferase 1 [Setaria italica] >gi|944227511|gb|KQK91915.1| hypothetical protein SETIT_036632mg [Setaria italica])

HSP 1 Score: 192.971 bits (489), Expect = 3.415e-56
Identity = 116/279 (41.58%), Postives = 153/279 (54.84%), Query Frame = 0
Query:    8 VTRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYG--LTVEELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVWQD--KRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALRACL 282
            +T  +AEDL++  V LA+EARDEFW +  R    K K          R LVAAS+G YGA  ADGSEY G+YG  +T E+L D+HR RL +L     DL+AFE IP + E  A+++LL E   Q          P WI  +  D  HL SGES   C+Q++ A +     +V  +GVNC  PQ +EG +   R                K +++ +  YPNSGEVW    KRWLP    C   + F  LA++W  AGA ++GGCCRTTP TI+A+   L
Sbjct:   81 MTLDEAEDLLQTSVKLALEARDEFWKSTLR----KSKP------IYNRALVAASIGSYGAYLADGSEYSGSYGADITTEKLKDFHRRRLQVLAGAGPDLIAFEAIPNKMEAQALVELLEEENIQ---------VPSWICFSSVDGKHLCSGESFADCLQILNASE-----KVAVVGVNCTPPQFIEGIICEFR----------------KQTKKAIAVYPNSGEVWDGIAKRWLPAE--CLGHKSFDALAKRWHEAGASLIGGCCRTTPSTIRAVSKIL 317          
BLAST of EWM29829.1 vs. NCBI_GenBank
Match: gi|1053899271|ref|WP_066096701.1| (homocysteine S-methyltransferase [Hydrogenophaga sp. LPB0072] >gi|1026338846|gb|OAD39332.1| homocysteine S-methyltransferase [Hydrogenophaga sp. LPB0072] >gi|1078664384|gb|AOW11492.1| homocysteine S-methyltransferase [Hydrogenophaga sp. LPB0072])

HSP 1 Score: 192.586 bits (488), Expect = 4.649e-56
Identity = 113/273 (41.39%), Postives = 152/273 (55.68%), Query Frame = 0
Query:    9 TRAQAEDLIRKGVALAIEARDEFWAAYSREEAGKRKTEEAGGGTRQRPLVAASVGCYGAAQADGSEYRGAYGLTVEELMDWHRPRLAILGETDADLLAFETIPCQAEVVAILQLLREGREQEGSAPTSRRTPCWITLACQDATHLNSGESLVSCVQLVKALDTGSRPQVVGLGVNCCAPQHVEGALTVLREGLLTISAAKEVISAAKGSERILIAYPNSGEVWQ--DKRWLPGTGVCPSSRDFADLARKWVVAGARVLGGCCRTTPETIKALR 279
            +RA A  L+R+ + LAIEARD+FWA              A    RQ+PLVAASVG YGA  ADGSEYRG YG+  + LMD+HRPR+A+L E  ADLLA ETIPC  E +AI +LL          P       WI+ +C+D  H + GE L  CV  + A+     PQ+  +G+NC AP+ +   +   R+                 + + ++ YPNSGE +    K W    GV  +++DFA  A +W   GAR++GGCCRT PE I+A+R
Sbjct:   88 SRADAAMLMRRSIELAIEARDQFWA------------NPANRIGRQKPLVAASVGPYGAMLADGSEYRGHYGVARQALMDFHRPRMAVLAEAGADLLACETIPCLDEAMAIAELL----------PDFGSISAWISFSCKDGEHNSQGERLADCVAALDAV-----PQIAAIGINCTAPEWIPSLVEQARQ----------------HTAKPIVVYPNSGEHYDATSKEW---HGVS-NAQDFAQQAMRWSQRGARLVGGCCRTGPEDIRAVR 313          
The following BLAST results are available for this feature:
BLAST of EWM29829.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585112410|gb|EWM29829.1|0.000e+0100.00homocysteine s-methyltransferase [Nannochloropsis ... [more]
gi|585112411|gb|EWM29830.1|6.074e-14099.50homocysteine s-methyltransferase [Nannochloropsis ... [more]
gi|553189163|ref|XP_005854945.1|1.208e-9999.32homocysteine S-methyltransferase, partial [Nannoch... [more]
gi|1082655086|gb|OFZ80118.1|1.128e-5743.37homocysteine S-methyltransferase [Bdellovibrionale... [more]
gi|504246229|ref|WP_014433331.1|2.201e-5741.88homocysteine S-methyltransferase [Caldilinea aerop... [more]
gi|1194436361|gb|OUC08407.1|2.883e-5743.22homocysteine methyltransferase [Litorilinea aeroph... [more]
gi|944227510|gb|KQK91914.1|5.193e-5740.86hypothetical protein SETIT_036632mg [Setaria itali... [more]
gi|1174567826|gb|OQU92985.1|7.588e-5741.73hypothetical protein SORBI_3001G453100 [Sorghum bi... [more]
gi|514820963|ref|XP_004985165.1|3.415e-5641.58PREDICTED: homocysteine S-methyltransferase 1 [Set... [more]
gi|1053899271|ref|WP_066096701.1|4.649e-5641.39homocysteine S-methyltransferase [Hydrogenophaga s... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna823rna823Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications