EWM22446.1, cds8187 (CDS) Nannochloropsis gaditana

BLAST of EWM22446.1 vs. NCBI_GenBank
Match: gi|585102952|gb|EWM22446.1| (mannosyl-oligosaccharide -alpha-mannosidase [Nannochloropsis gaditana])

HSP 1 Score: 1587.78 bits (4110), Expect = 0.000e+0
Identity = 768/768 (100.00%), Postives = 768/768 (100.00%), Query Frame = 0

Query:    1 MLRSSRGYRHFEKSSSIDRTSKRLGVVIYLSFALLLGALPWLLWPGTAPHGDRTGPGNMLLFAVAENSPDSNTATFHGAEPAAAEHKSATLSTQAEDLRDPLIDAERIRENDEGKDKMGETEETQNQNDEKAGKEGGVKVAAEKVAAPATEEELEKIVQDVEENEMNSSKNKKGEEKLPAAELNGDVIGVPDAKKLEKGTKGYKKSIAATLKTEAEKKRKPQENRPDQTEADAEKDTRDQVIVKIANKETGKVLATSVRKADKDPFVRLAPQDPHIDKTLAKERRMRVKTAMKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAGLPSGSHQACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEKGLFRLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKETWLREMFDESVDGAEELLIARGPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVFDQDGVPQASDPRYILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGEVDLSRQVFNTEAHPLHIFDE 768
            MLRSSRGYRHFEKSSSIDRTSKRLGVVIYLSFALLLGALPWLLWPGTAPHGDRTGPGNMLLFAVAENSPDSNTATFHGAEPAAAEHKSATLSTQAEDLRDPLIDAERIRENDEGKDKMGETEETQNQNDEKAGKEGGVKVAAEKVAAPATEEELEKIVQDVEENEMNSSKNKKGEEKLPAAELNGDVIGVPDAKKLEKGTKGYKKSIAATLKTEAEKKRKPQENRPDQTEADAEKDTRDQVIVKIANKETGKVLATSVRKADKDPFVRLAPQDPHIDKTLAKERRMRVKTAMKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAGLPSGSHQACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEKGLFRLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKETWLREMFDESVDGAEELLIARGPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVFDQDGVPQASDPRYILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGEVDLSRQVFNTEAHPLHIFDE
Sbjct:    1 MLRSSRGYRHFEKSSSIDRTSKRLGVVIYLSFALLLGALPWLLWPGTAPHGDRTGPGNMLLFAVAENSPDSNTATFHGAEPAAAEHKSATLSTQAEDLRDPLIDAERIRENDEGKDKMGETEETQNQNDEKAGKEGGVKVAAEKVAAPATEEELEKIVQDVEENEMNSSKNKKGEEKLPAAELNGDVIGVPDAKKLEKGTKGYKKSIAATLKTEAEKKRKPQENRPDQTEADAEKDTRDQVIVKIANKETGKVLATSVRKADKDPFVRLAPQDPHIDKTLAKERRMRVKTAMKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAGLPSGSHQACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEKGLFRLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKETWLREMFDESVDGAEELLIARGPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVFDQDGVPQASDPRYILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGEVDLSRQVFNTEAHPLHIFDE 768          

BLAST of EWM22446.1 vs. NCBI_GenBank
Match: gi|298709031|emb|CBJ30981.1| (Mannosyl-oligosaccharide 1,2-alpha-mannosidase, family GH47 [Ectocarpus siliculosus])

HSP 1 Score: 380.178 bits (975), Expect = 3.165e-117
Identity = 208/489 (42.54%), Postives = 280/489 (57.26%), Query Frame = 0

Query:  280 LAKERRMRVKTAMKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAGLPSGSHQACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEKGLFRLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKETWLREMFDESVDGAEELLIARGPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVFDQDGVPQASDPR--YILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGEVDLSRQVFNTEAHPLHIF 766
            L +ER   ++ AM   W  Y  HAWG D + P+S   ++ WGGM +T++DSL TLW+MGL EEF   RDW+ ++L+F  VG +SVFET IR LGGLLSAF+LSRD+VFLE+A +LAD L+PAF + TG P   + LS G   G   + L+E GT+QLEFRYLSH  G+  YA+ AM  ++ +   +      GL+ +  N +TG +     + GA GDSFYEYLLK W+QG ++E   R M+++++DG  ELL+ +   G  Y++  +   +  RM+HLACF  G LALGA T          +RA   +   + L  TCYQ      TGL+P+ V F+  G  +A D    YILRPE  ES + LH L GDP +RE+G ++F AI+ +CR   AY      S P                DV   G               +  +D MESFF  ETLKYLYL+ + +   +DL     NTEAHPL IF
Sbjct:  297 LGRERAAVIRAAMDLLWKGYSEHAWGYDEVKPLSKKGSDNWGGMGVTLVDSLDTLWLMGLTEEFYNGRDWVRDHLTFNEVGMVSVFETTIRVLGGLLSAFELSRDEVFLERAKDLADRLMPAFGTSTGIPYKLVNLSNGKTGGGSYSVLSELGTLQLEFRYLSHEVGDEKYARTAMRAFDFL---AGRTVPHGLYPIRINPNTGKFTSSQVTFGALGDSFYEYLLKVWMQGGRREESYRRMYEDAMDGVAELLVQKAR-GLTYLSEWNGTRRQHRMDHLACFLAGNLALGAMTSHDS------RRAARDLRTGKALAYTCYQMYLRTATGLSPEVVEFNGAGDMRARDQARFYILRPETLESFFVLHQLTGDPVYREWGWEIFRAIERHCRVGVAYG-----SHP----------------DVENPG---------------LEAKDHMESFFPGETLKYLYLLMQPDH-PIDLMEYTLNTEAHPLKIF 738          

BLAST of EWM22446.1 vs. NCBI_GenBank
Match: gi|224000011|ref|XP_002289678.1| (mannosyl-oligosaccharide 1,2-alpha-mannosidase [Thalassiosira pseudonana CCMP1335] >gi|220974886|gb|EED93215.1| mannosyl-oligosaccharide 1,2-alpha-mannosidase [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 352.443 bits (903), Expect = 2.380e-110
Identity = 207/487 (42.51%), Postives = 276/487 (56.67%), Query Frame = 0

Query:  292 MKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAGLPS----GSHQACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEK-GLFRLDFNSDTGA--WGGKHYSVGARGDSFYEYLLKTWLQGNKKETWLREMFDESVDGA-EELLIARGPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVF--DQDGVPQASDPRYILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGEVD-LSRQVFNTEAHPLHIFD 767
            MK  W  Y  +A+GKD + PIS  S N WGGM  +++DSL TLW+M LK+EF +ARDW+ E+LS   VG +S FET IR+LGGLLSA+DLSRD VFLEKA +L   L  +F++P+G P   + L  G         +   LAE GTVQ+E+RYL+HATG   YA K+    ER+ +  AD+  K GL   +     G+  +     S GA GDS YEY++K WLQG +KE   R+M+D S+ G  E+LL    P G  YIA+  +G    +M+HL CF  G LA+GA T     D+    RA   +  A+ L  TCYQ      TG+AP+ V F    D    +S P YILRPE  E+ YYL +L GDP +RE+G ++F++I+ YCR    Y  +                                    K  D   +  ED MESFF+AETLKY YL+ + +  E+D L++ VFNTEAHP+ IF+
Sbjct:    1 MKHVWKNYKEYAFGKDELHPISHKSTNNWGGMGTSLVDSLDTLWLMDLKDEFYEARDWVRESLSHSHVGDVSAFETTIRSLGGLLSAYDLSRDAVFLEKAEDLGSRLFKSFNTPSGLPHGSVNLQTGTSHNFGWNGNAYILAEIGTVQIEYRYLAHATGKTEYATKS----ERVFDILADIMPKDGLLSQNLKDKGGSAYFSNDKVSFGAMGDSTYEYMIKMWLQGGRKEQKYRDMWDHSMSGVHEQLLQKSSPNGLSYIADRVSGRIDHKMDHLVCFMGGALAIGAYTDPLGFDS---PRAQRDLKTAKALTYTCYQMYARSKTGIAPEFVRFAGGNDFEIPSSAPFYILRPETVEAFYYLSVLTGDPIYREWGWEIFQSIEKYCRVQYGYGSL------------------------------------KHVDNVNMNPEDRMESFFMAETLKYFYLLFDPD-SEIDILNKHVFNTEAHPMKIFE 443          

BLAST of EWM22446.1 vs. NCBI_GenBank
Match: gi|224003517|ref|XP_002291430.1| (mannosyl-oligosaccharide alpha-mannosidase, partial [Thalassiosira pseudonana CCMP1335] >gi|220973206|gb|EED91537.1| mannosyl-oligosaccharide alpha-mannosidase, partial [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 349.362 bits (895), Expect = 3.808e-109
Identity = 207/491 (42.16%), Postives = 284/491 (57.84%), Query Frame = 0

Query:  288 VKTAMKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSV-GHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAG---LPSGSHQ--ACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEKGLF--RLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKETWLREMFDESVDGAEELLI-ARGPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVFD-QDGVPQASD-PRYILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGEVDLSRQVFNTEAHPLHIFD 767
            VK AM+ AWN Y  +AWGKD +LP++    N WGG   T++DSLSTLW+MG+K+EF +ARDWI +NL F  V G +SVFET IR LG LLSAFD+S DKVFLEKA +L + L+ AFD+P G P   + L  G     +G H+  A L+E GT+Q+EFRYL+  TG   YA  AM   + + +      E GL+   +    ++ ++     S+GA GDSFYEYLLK WLQG K E   R ++D +VDG  + L+ +  P    Y+A    G    +M+HL+CF  G LALGA    T  D +   RA   + + ++L  TCYQ      +GL+P+ V F+  D   +A D P Y LRPE +E+ + L+ L  DP +RE+G ++F+AID++CRT+  YA +                                      KD+  + + + MESFF+AETLKYLYL+Q    G   L++ VFNTEAHPL + D
Sbjct:    1 VKEAMRHAWNGYRKYAWGKDEVLPLTHGGQNNWGGQGTTLVDSLSTLWMMGMKDEFNEARDWIRDNLDFSKVEGAVSVFETTIRNLGSLLSAFDMSGDKVFLEKADDLGNRLMRAFDTPRGVPFGEVELFDGGSAYNTGWHKTSAVLSEIGTLQVEFRYLARVTGKSEYATVAMRALDELLKLD---TESGLYPTFIHNTKNSLSFANNDISIGAMGDSFYEYLLKIWLQGGKTEGNYRILYDRAVDGILDYLVHSSRPNYLTYVAELSQGKVKHKMDHLSCFLGGNLALGAY---THPDGLASMRAQRQLKVGKQLAYTCYQMYARSKSGLSPEYVSFEGSDDFVKAHDAPYYNLRPETSETFFILYHLTKDPVYREWGWEVFQAIDWHCRTDAGYAAI--------------------------------------KDVDTMQKNNRMESFFIAETLKYLYLLQGDSHGLDLLNKHVFNTEAHPLRLLD 447          

BLAST of EWM22446.1 vs. NCBI_GenBank
Match: gi|299471205|emb|CBN79061.1| (Mannosyl-oligosaccharide 1,2-alpha-mannosidase, family GH47 [Ectocarpus siliculosus])

HSP 1 Score: 361.688 bits (927), Expect = 3.207e-108
Identity = 204/481 (42.41%), Postives = 271/481 (56.34%), Query Frame = 0

Query:  271 PQDPHIDKTLAKERRMRVKTAMKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAGLPSGSHQACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEKGLFRLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKETWLREMFDESVDG-AEELLIARGPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVFD--QDGVPQASDPRYILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGE 748
            PQ         +ER  +VK AM+ AW  Y  HAWG D + P +      WGGM +T++DSL TLW+MG+KEEF +ARDW+ + L+F     +SVFET IR LGGLLSA+DLS+D++F  KA ELAD+LLPAFD+ TG P   +   +   SG  ++ LAE GT+Q+EFRYLS  TG P YA K   V++ M+      ++ GL+ +  N   G   G   + GA GDSFYEYLLK WLQG K ET  R M+D ++DG   +LL    P G  Y+++        +M+HL CF  GTLALG+ T +   D V   RA   +  A+ L  TCYQ    Q TGLAP+ V F+  +D V        ILRPE AE++Y LH + GDP +R++   +F+A + +CR   A+      + P+           R+P                         ED MESFFLAETLKYLYLIQ+ +  E
Sbjct:  425 PQSKEESDRKGRERAAKVKEAMQHAWKGYEQHAWGADELAPRAKKGKQPWGGMGVTLVDSLDTLWLMGMKEEFYRARDWVKDKLTFSHASEVSVFETTIRELGGLLSAYDLSKDEIFKTKAIELADLLLPAFDTNTGIPTARVSFRSHKNSGGSKSVLAEIGTLQVEFRYLSKITGIPKYADKVNKVFDHMY---GLPSKDGLYPIFVNPSNGQTVGSQVTFGALGDSFYEYLLKAWLQGGKTETKYRTMYDRAMDGMTSKLLRKSDPSGLTYVSDLSGSKNVDKMDHLVCFLAGTLALGSKTTD---DPV---RADRDLKTAKALAYTCYQMYERQKTGLAPEMVTFEKGKDMVVPPKAAYNILRPETAEALYVLHQITGDPIYRDWSWNIFQAFEKHCRLPVAFGSFPNVNDPN-----------RQP-------------------------EDRMESFFLAETLKYLYLIQDPDQKE 860          

BLAST of EWM22446.1 vs. NCBI_GenBank
Match: gi|219114373|ref|XP_002176357.1| (predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1] >gi|217402603|gb|EEC42593.1| predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 345.125 bits (884), Expect = 6.156e-107
Identity = 213/508 (41.93%), Postives = 283/508 (55.71%), Query Frame = 0

Query:  280 LAKERRMRVKTAMKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFD-SPTGFPINGLILSAG----LPSGSHQACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESA-----------DVAEKGLFRLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKETWLREMFDESVDGA-EELLIARGPGGRLYIA--NNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVF--DQDGVPQASDPRYILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGEVD-LSRQVFNTEAHPLHI 765
            +A+ RR  V+ AM+  W  Y  +A+GKD + P S + +++WGG  +T++DSL TLW+M LK+EF QARDW+ ++L   +VG +SVFET IR LGGLL+AFD S+D+VFL+KA +L D LL AFD + TG P   + L++G    +      A +AEFGTVQ+E RYLS  T +  YAKK   VYE + E S            D   KG F  D  +    +     + GA  DS YEY LKTWLQG + E   R M+D+S+ G  ++LL    P    YIA  N ++G    +M+HL CF  G LALGA T     D+    RA   +  A+ L  TCYQ      TG++ + V F   +D       P Y+LRPE  ES + LH L GDP +RE+G ++F+AI+ YC+T   Y                  GA    +DV G+                    D MESFFLAETLKYLYL+Q+ +  EVD L + VFNTEAHPL I
Sbjct:   22 VARSRRFHVQKAMRFVWGNYVKYAFGKDELKPQSASGSDQWGGQGITLVDSLDTLWLMNLKDEFWQARDWVRDHLDHSTVGSVSVFETTIRDLGGLLAAFDWSKDRVFLDKAVDLGDRLLRAFDGATTGIPWGEVNLASGNRKNIGWSGDNAIVAEFGTVQIENRYLSKLTADDKYAKKTERVYEILDEISYPDGLYPYYLRNDPRNKGKFLRDGVTIRPEFSNDKLTFGAMADSLYEYFLKTWLQGGRTEPLYRAMYDKSIQGMHDQLLQTSTPSKLTYIADKNRNSGTMDHKMDHLVCFMGGLLALGAYTDPLGLDST---RAQRDLQTAKALTYTCYQMYARMNTGISAEYVQFYAGRDFQIGRGAPHYLLRPEAVESFFILHQLTGDPVYREWGWEVFQAIEKYCKTPYGY------------------GALNNVADVNGK------------------PRDSMESFFLAETLKYLYLLQDPDT-EVDVLHKHVFNTEAHPLRI 489          

BLAST of EWM22446.1 vs. NCBI_GenBank
Match: gi|676384617|ref|XP_009035587.1| (hypothetical protein AURANDRAFT_71340 [Aureococcus anophagefferens] >gi|323453654|gb|EGB09525.1| hypothetical protein AURANDRAFT_71340 [Aureococcus anophagefferens])

HSP 1 Score: 362.459 bits (929), Expect = 1.223e-106
Identity = 211/492 (42.89%), Postives = 279/492 (56.71%), Query Frame = 0

Query:  281 AKERRMRVKTAMKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAG----LPSGSHQACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEKGLFRLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKETWLREMFDESVDGAEELLIAR-GPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVFDQ--DGVPQASDPRYILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDD-MESFFLAETLKYLYLIQETEPGEVDLSRQVFNTEAHPLH 764
            A  R  +V+ AM+ AW  Y   AWG+D + P+S    + WGGM +T++DSL TLW+MGL++EF++A+ W+ ++L F   G +SVFET IR LGGLL+AFDLS DKVFL+KA +L   L  AFD+PTG P     L  G    +      A LAE G+V+LEFRYL +  G+  ++ KA  V+E+    S     +GLF +  +   G    +  + GA GDS+YEYLLKTWLQG + ET LREM+DE++DG  +LL+    P    Y+A+ D G    +M+HL CF  G LALGA T     D+   +R  H   +A  L  TC Q    Q T LAP+ V F +  D V   S P YILRPE AES++ LH +  +P +R++G ++F AI+  CRT   Y                                      G   D+  VT+ DD MESFFLAETLKYLYL+Q+     VDL   VFNTEAHPL 
Sbjct:  345 AAGRAAKVRDAMRHAWKGYKDRAWGRDELKPVSGRGHDNWGGMGVTLVDSLDTLWLMGLRQEFDEAKKWVRDSLRFDRTGDVSVFETTIRELGGLLAAFDLSGDKVFLDKARDLGQRLAHAFDTPTGIPKGSTSLREGRAKDVGWTGGNAVLAELGSVELEFRYLGYKLGDASFSDKANRVFEQF---STMKGTQGLFPIYVDPRNGGLKSRKITFGALGDSYYEYLLKTWLQGGRTETRLREMYDEAMDGMVDLLLQHSSPSNLAYVADIDGG-TVHKMDHLVCFLGGVLALGAKTDPRGFDSPRARRDLH---VAEALTHTCVQMYLRQPTKLAPEFVTFRRGADMVVAPSAPFYILRPETAESLFVLHEVTKNPVYRDWGWEIFSAIEARCRTAHGY--------------------------------------GSVPDVRKVTKPDDRMESFFLAETLKYLYLLQDPNHA-VDLETAVFNTEAHPLR 790          

BLAST of EWM22446.1 vs. NCBI_GenBank
Match: gi|1002264486|ref|XP_015636580.1| (PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1 [Oryza sativa Japonica Group] >gi|38346546|emb|CAD41779.2| OSJNBa0035M09.7 [Oryza sativa Japonica Group] >gi|113565371|dbj|BAF15714.1| Os04g0606400 [Oryza sativa Japonica Group] >gi|222629502|gb|EEE61634.1| hypothetical protein OsJ_16076 [Oryza sativa Japonica Group] >gi|937915972|dbj|BAS90891.1| Os04g0606400 [Oryza sativa Japonica Group])

HSP 1 Score: 330.102 bits (845), Expect = 4.289e-100
Identity = 202/504 (40.08%), Postives = 277/504 (54.96%), Query Frame = 0

Query:  268 RLAPQDPHIDKTLAKERRMRVKTAMKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAGL---PSGSH-QACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEKGLFRLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKE--TWLREMFDESVDGAEELLIARGPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVFDQDGVPQASDPRYILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGEVDLSRQVFNTEAHPLHI 765
            +L   DP     +  ERR +VK AM  AWN+Y  +AWG D + P S    N +GG+  T++DSL TL++MGLK+EF++ARDW+A++LSF      SVFET IR +GGLLSA+DLS DKVFLEKA ++ D LLPA+D+P+G P N + L+ G    P  ++  + LA+ GT QLEF  LS  TG+P Y +KA +V  ++ +        GL  +  N  +G       + GA GDSFYEYLLK W+QGNK E     R+M++ S++G   L     P    YI   + G  + +M+ LACF PG LALGA+  E        ++A   M+LA+EL  TCY F  +  T LA ++  F             ILRPE  ES+ YL  L G+  ++++G  +F+A +            R+ SG  G R V                      +G+  +M        M+SFFLAETLKYLYL+  + P  +     VFNTEAHPL I
Sbjct:   92 KLVEIDP-----INNERREKVKEAMAHAWNSYVKYAWGMDELQPQSKNGVNSFGGLGATLVDSLDTLYIMGLKDEFQRARDWVADSLSFDKDYDASVFETTIRVVGGLLSAYDLSGDKVFLEKAKDITDRLLPAWDTPSGIPYNRINLAHGRAHNPGWTNGDSILADSGTEQLEFIALSQRTGDPKYQQKAENVIRQLQK---IYPSDGLLPIYINPHSGTASYSTITFGAMGDSFYEYLLKVWVQGNKTEHVKHYRQMWETSMEGLLSLTKKTTPSNYYYICEKNGGSLSDKMDELACFAPGMLALGASGYEET------EKAEEIMNLAKELARTCYNFYQTTPTKLAGENYFFHTGQDMNVGTSWNILRPETVESLMYLWRLTGNKTYQDWGWDIFQAFE---------KNSRIESGYVGLRDV---------------------NTGEKDNM--------MQSFFLAETLKYLYLLF-SPPSVISFDEWVFNTEAHPLRI 542          

BLAST of EWM22446.1 vs. NCBI_GenBank
Match: gi|951005236|ref|XP_014508082.1| (PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1-like isoform X2 [Vigna radiata var. radiata])

HSP 1 Score: 328.176 bits (840), Expect = 6.405e-100
Identity = 206/515 (40.00%), Postives = 288/515 (55.92%), Query Frame = 0

Query:  258 VRKADKDPFVRLAPQDPHIDKTLAKERRMRVKTAMKRAWNAYHAHAWGKDTILPISLTSANRWGGMAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAGLPSGSHQA----CLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEKGLFRLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKE--TWLREMFDESVDGAEELLIARGPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVFDQDGVPQASDPRY-ILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVRLSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGEVDLSRQVFNTEAHPLHI 765
            V K  KD +V    +DP     ++K+RR ++K AM  AW +Y  +AWGKD + P S+   + +GG+  T++DSL TL+VMGL ++F +AR+W+AE+LSF     +SVFET IR +GGLLSA+DLS DKVFLEKA E+AD LLPA+++P+G P N L L+ G  +    A     LA+ G+ QLEF  LS  T +P Y +KA  V + +H+   D    GL  +  N  TG   G   + GA GDSFYEYLLK W+QGNK E   + REM+++S+ G + ++    P    YI+         +M+ LACF PG LALG++   T G    EK    Y+ LA EL  TCY F  S  T LA ++  F +DG   +    + I RPE  ES++YL    G+  ++E+G  +F+A +            R+ +G  G + V                      +GK  +M        M+S+FL+ETLKYLYL+  +    + L   VFNTEAHPL I
Sbjct:   61 VNKFAKDIYV---DEDP-----ISKQRRDKIKDAMLHAWTSYETYAWGKDELKPQSMNGVDSFGGLGATIVDSLDTLFVMGLDDQFNRAREWVAESLSFDKNIEVSVFETTIRVMGGLLSAYDLSGDKVFLEKAREIADKLLPAWNTPSGIPYNRLNLAFGNTNNPGWARGNSILADSGSEQLEFIALSQRTKDPKYQEKAEKVIKGLHKSFPD---DGLLPIYLNPLTGTQTGGSVTFGAMGDSFYEYLLKAWIQGNKTEAVAFYREMWEKSMIGLQSMIKKSTPSSFTYISEKLGNAVFDKMDELACFVPGMLALGSS---TSGPGEAEK----YLSLAEELVWTCYNFYQSTPTKLAGENYYF-RDGEDMSVGTTWNIQRPETIESLFYLWRFTGNKTYQEWGWNIFQAFE---------NNTRIETGYVGLKDVT---------------------TGKKDNM--------MQSYFLSETLKYLYLLF-SPSSVISLDEWVFNTEAHPLRI 517          

BLAST of EWM22446.1 vs. NCBI_GenBank
Match: gi|678198791|gb|KFV65773.1| (Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, partial [Picoides pubescens])

HSP 1 Score: 326.635 bits (836), Expect = 1.515e-99
Identity = 193/511 (37.77%), Postives = 279/511 (54.60%), Query Frame = 0

Query:  271 PQDPHIDKTLAKERRMRVKTAMKRAWNAYHAHAWGKDTILPISLT--SANRWGG--MAMTMLDSLSTLWVMGLKEEFEQARDWIAENLSFKSVGHISVFETNIRALGGLLSAFDLSRDKVFLEKAAELADMLLPAFDSPTGFPINGLILSAGLP-----SGSHQACLAEFGTVQLEFRYLSHATGNPIYAKKAMHVYERMHEESADVAEKGLFRLDFNSDTGAWGGKHYSVGARGDSFYEYLLKTWLQGNKKETWLREMFDESVDGAEELLIARGPGGRLYIANNDNGMQAKRMEHLACFYPGTLALGAATLETKGDAVLEKRATHYMDLARELGETCYQFAGSQMTGLAPDSVVFDQDGVP----QASDPRYILRPEVAESMYYLHLLDGDPKWREYGAKLFEAIDFYCRTNEAYAQVR--LSSGPSGRRSVALRGAERKPSDVGGEGASQAEQSGKAKDMGLVTQEDDMESFFLAETLKYLYLIQETEPGEVDLSRQVFNTEAHPLHIF 766
            P DP +     +ERR ++K  MK AW+ Y  + WG + + PI+    S N +G   M  T++D+L TL++MGL +EF + ++WI +NL F     +SVFE NIR +GGLL+A+ LS  +VF  KA +LA  LLPAF++PTG P   + L +G+      + +  + LAEFGT+ +EF +LS+ TG+P+Y  K MH+ + + +        GL+    N  TG WG  H SVG  GDSFYEYLLK WL  +K +T  R+M+D++++  E+ LI +  GG  +I    NG   ++M HL CF  G  ALGA       D   E +A HY+ L  E+  TC++        L P++  FD  GV     + ++  YILRPEV E+ +Y+     DPK+R++G +  +AID YCR +  ++ V+   SS P                                      T +D  +SFFLAETLKYLYL+   +   + L   VFNTEAHPL + 
Sbjct:   63 PSDPDV-----RERRNKIKEMMKHAWDNYRQYGWGHNELKPIARKGHSTNIFGNSQMGATIVDALDTLYIMGLHDEFREGQEWIDKNLDFSVNSEVSVFEVNIRFIGGLLAAYYLSGQEVFKIKAVQLAGKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFVHLSYLTGDPVYYNKVMHIRKLLQKMD---RPNGLYPNYLNPRTGRWGQHHTSVGGLGDSFYEYLLKAWLMSDKMDTEARKMYDDAIEAIEKHLIRKSNGGLTFIGEWKNGHLERKMGHLTCFAGGMFALGA-------DGSREDKAGHYLQLGAEIAHTCHESYDRTTLKLGPEAFKFD-GGVEAVAVRQNEKYYILRPEVIETYWYMWRFTHDPKYRQWGWEAAQAIDKYCRVSGGFSGVKDVYSSSP--------------------------------------TYDDVQQSFFLAETLKYLYLLFSNDD-LLPLDNWVFNTEAHPLPVL 518