EWM22466.1, cds8170 (CDS) Nannochloropsis gaditana

BLAST of EWM22466.1 vs. NCBI_GenBank
Match: gi|585102972|gb|EWM22466.1| (major facilitator superfamily mfs_1 [Nannochloropsis gaditana])

HSP 1 Score: 892.108 bits (2304), Expect = 0.000e+0
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query:    1 MPPPHSSFLSPLRMLLLSNALEMLAVALVLPAYPDLTKALGISLQTRGLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFILLLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTPFLGGYLLERHGGHALGFVSALFLLPVCAHVAFTEFFKVRKVVQEDKKEK 442
            MPPPHSSFLSPLRMLLLSNALEMLAVALVLPAYPDLTKALGISLQTRGLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFILLLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTPFLGGYLLERHGGHALGFVSALFLLPVCAHVAFTEFFKVRKVVQEDKKEK
Sbjct:    1 MPPPHSSFLSPLRMLLLSNALEMLAVALVLPAYPDLTKALGISLQTRGLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFILLLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTPFLGGYLLERHGGHALGFVSALFLLPVCAHVAFTEFFKVRKVVQEDKKEK 442          

BLAST of EWM22466.1 vs. NCBI_GenBank
Match: gi|553184243|ref|XP_005854058.1| (major facilitator superfamily mfs 1 [Nannochloropsis gaditana CCMP526] >gi|422295006|gb|EKU22305.1| major facilitator superfamily mfs 1 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 666.766 bits (1719), Expect = 0.000e+0
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query:  115 ISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFILLLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTPFLGGYLLERHGGHALGFVSALFLLPVCAHVAFTEFFKVRKVVQEDKKEK 442
            ISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFILLLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTPFLGGYLLERHGGHALGFVSALFLLPVCAHVAFTEFFKVRKVVQEDKKEK
Sbjct:    9 ISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFILLLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTPFLGGYLLERHGGHALGFVSALFLLPVCAHVAFTEFFKVRKVVQEDKKEK 336          

BLAST of EWM22466.1 vs. NCBI_GenBank
Match: gi|503325107|ref|WP_013559768.1| (MFS transporter [Anaerolinea thermophila] >gi|319994610|dbj|BAJ63381.1| major facilitator superfamily transporter [Anaerolinea thermophila UNI-1])

HSP 1 Score: 167.162 bits (422), Expect = 1.412e-43
Identity = 116/391 (29.67%), Postives = 198/391 (50.64%), Query Frame = 0

Query:   11 PLRMLLLSNALEMLAVALVLPAYPDLTKALGISLQTRGLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFILLLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTPFLGGYLLE 401
            PL  + L   +++L  +L+LP  P L K+   S    GL+V+ ++L QF+ +P LGR SD  GR  +L +S   +   +  + +A++     L R+L GF   +ISV+QAYISD++  Q RA+ +G++G+ FGL FI+GPA+GG L         + A   +A NF+L+ S  + S             ER+            +  N      +  K  + +  +  V      GP       LL  + F  ++  +F+T F+ YA +   +  +  G +L+++GV+SV+     +  L+  F + QL+F AI+  AFS   WG   ++  LL  L  + ++G I  T++++ LS        G +LG+S ++E+  +++ P LG  LL+
Sbjct:    6 PLSSIFLVVFIDLLGFSLILPLLPYLAKSFSASEFQIGLLVASYALAQFIGAPFLGRLSDRFGRRPILLISIAGNAIGFFLLGIAQNLEMLFLSRILAGFTAANISVAQAYISDVTDAQSRARGLGLIGAAFGLGFILGPAIGGALSQFGYAYPAFAATGLSALNFLLVFSWLKESLT----------PERR------------QALNHQQRPPITLKAMLETLRRPYV------GP-------LLHTRFFFGLAFSLFQTIFSLYALERFNLQARDTGYILAYVGVISVLTQGVFIGKLTRKFNEIQLMFVAILTMAFSLLAWGFAPSVPVLLITLLPIAMSGGILNTVINSALSKSVAPIETGGILGLSASLESLTRVIAPSLGTLLLQ 361          

BLAST of EWM22466.1 vs. NCBI_GenBank
Match: gi|1082225075|gb|OFW46407.1| (hypothetical protein A3J29_17705 [Acidobacteria bacterium RIFCSPLOWO2_12_FULL_67_14b])

HSP 1 Score: 135.961 bits (341), Expect = 2.991e-32
Identity = 112/401 (27.93%), Postives = 184/401 (45.89%), Query Frame = 0

Query:    9 LSPLRMLLLSNALEMLAVALVLPAYPDLTKALGISLQTRGLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFI-LLLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQL----IFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTPFLGGYLLERHG 404
            +SPL ++ ++  +++L   +++P  P   ++ G S    GL+ + FSL+QF+ SPI GR SD +GR  ++ +  + S   Y+ + +A       + R+L G    +I  +QAYI+D+++P+ RAK MGM+G+ FGL FI GPA+GG L    P   +W A     +NFI     LPE                R A   NP+ +G  R                         D  RH      + +LLA    V  +   FE +FA ++    G    ++G L +++GVV  +    +V  +     + +L    IF   VG     F W    ++ +LL  LA L +        LS  +S        G  LG++ ++ +  +++ P  GGYL + +G
Sbjct:    1 MSPLAVIFVTVFIDLLGFGIIIPLLPFYAESFGASAFAIGLLGTSFSLMQFVFSPIWGRWSDRIGRKPIILLGLMGSCVSYLTLALATSLTLVFVARILGGIAGANIPTAQAYIADVTTPENRAKGMGMVGAAFGLGFIFGPAIGGVLSRISPEAPMWTASALCLSNFIAAWFLLPE---------------SRAA---NPETKGLGRL------------------------DAFRHAMTKPTLVLLLALYFIVTAAFSGFEATFALFSESRFGFTAASIGFLFAYIGVVLALVQGVLVHKVVKRVGEARLIPVAIFAIAVGVGLVPFAW----SVPTLLVALAVLALGMGFNSPALSAMVSKTADADDQGGTLGLASSLASLGRVVGPAWGGYLYDAYG 355          

BLAST of EWM22466.1 vs. NCBI_GenBank
Match: gi|1169633358|ref|WP_080507469.1| (MFS transporter [Bryobacter aggregatus])

HSP 1 Score: 130.183 bits (326), Expect = 8.936e-30
Identity = 113/405 (27.90%), Postives = 184/405 (45.43%), Query Frame = 0

Query:   21 LEMLAVALVLPAYPDLTKALGISLQTRGLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVA-LLATAANFILLLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTPFLGGYLLERHGGHALGFVSALFLLPVCAHVA 424
            L++L + +++P  P   +       T GL+ ++FS+ QF SSP+LG  SD  GR  ++  + + S F Y     A      ML RV+ G    SIS +QAYI+D++ P+ R K  G++G++FG+ FIIGPA GGFL S    +  + A  L  AA       LPE    S P        ER+   P        R A+AN    +      A+  +D +  ++         A+      F A+     +++FA +  K  GM  Q    +  ++GV+SV+    +VR L   +    L    ++G A              L P +A +     +   +L+  +S     +  GTVLG + +I +  +I+ P   GY+ +  G  A  ++ A+ ++   A VA
Sbjct:   64 LDVLGLGVLIPVLPYYAQRFSADAMTVGLLSAVFSVAQFTSSPLLGAISDRYGRRRVILANILGSAFGYFLFGWASTLWVLMLARVIEGVTGGSISTAQAYIADVTPPEDRMKRFGLIGAVFGVGFIIGPAFGGFLSSYSLAMPAYAAGSLCLAAAIFGYFMLPE----SLP-------VERRIQTP-------LRLADANPIRQI-----AAALQRDSIRAIL--------FAIFAVNVAFSAL-----QSNFAFFTMKRFGMGPQQNAWIFVYIGVLSVLMQGVVVRRLPKHWNPLSLCITGLIGTAIGLLGISFSQEEWQLFPSIAVMAFGNGVAAPMLTALVSRRVGASEQGTVLGSTQSILSSTRIVGPIFAGYVFDAFGSGAPYWMGAILVVAGVAIVA 432          

BLAST of EWM22466.1 vs. NCBI_GenBank
Match: gi|501377102|ref|WP_012408668.1| (MFS transporter [Nostoc punctiforme] >gi|186464857|gb|ACC80658.1| major facilitator superfamily MFS_1 [Nostoc punctiforme PCC 73102])

HSP 1 Score: 128.257 bits (321), Expect = 2.582e-29
Identity = 121/433 (27.94%), Postives = 201/433 (46.42%), Query Frame = 0

Query:   11 PLRMLLLSNALEMLAVALVLPAYPDLTKALGISLQTRGLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFILLLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITG-SIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTPFLGGYLLERHGGHALGFVSALFLLPVCAHVAFTEFFKVRKVVQEDKKEK 442
            P+ +L L+  +++L   ++LP  P   +  G       L+V+++SL+QFL +P+ GR SD  GR  +L ++   S+  Y  +  A       L R L G    +IS +QAYI+DI++P  RA+ MG++G+ FGL FI+GPA+GGFL+  DP            ANF L      PS  S   +L    +   A    P+   SE KA          + ++   S            Q  Q  VL+    FV  +    +++ A ++ + L    Q    L +F+G+VS +    ++ +L   F + +L+   I+G        G   +L  LL V  +L   G S+ Q +L++ +S +T     G +LGI+ +    A+I+ P   G    + G  +  F+S   ++ V   ++     +V K   E  KE+
Sbjct:    9 PILVLFLTVFIDLLGFGIILPILPLYAEQFGAKPNEATLLVAIYSLMQFLFAPLWGRFSDRYGRRPILLLTLFGSVIAYAGLGFANSLWMLFLARSLAGIMAGNISTAQAYIADITTPANRARGMGIIGAAFGLGFILGPAIGGFLIGSDP----------DNANFHL------PSLFSGGLSLF---ALLCALTLLPESLNSETKAKMQANRHRRRRLNLLQLS------------QRPQFCVLVGIYFFVTFAVAAMDSTLALWSKQQLNWGPQQTSYLFAFMGIVSTIVQGGLIGFLKKHFGEIKLLTLGILGLGLGLLLIGFSQSLILLL-VATTLVAWGISVSQPILNSLISQMTAPEEQGQILGIASSCSALARIVGPTWAGISFMKFGSDS-PFLSGFLVMIVALALS----LRVTKSASESNKER 404          

BLAST of EWM22466.1 vs. NCBI_GenBank
Match: gi|500219918|ref|WP_011890043.1| (tetracycline resistance MFS efflux pump [Chlorobium phaeovibrioides] >gi|145205775|gb|ABP36818.1| major facilitator superfamily MFS_1 [Chlorobium phaeovibrioides DSM 265])

HSP 1 Score: 127.487 bits (319), Expect = 7.543e-29
Identity = 114/407 (28.01%), Postives = 185/407 (45.45%), Query Frame = 0

Query:   10 SPLRMLLLSNALEMLAVALVLPAYPDLTKALGISLQTRGLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTV-WVALLATAANFIL-LLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPV----LASLTITGSIFQTLLSTELSTVTPHALV-----------GTVLGISYAIETFAKILTPFLGGYL 399
            SPL +LLL+  L+++   +VLP  P   K LG S    GL+ ++FS++QF+ SP+ G+ SD +GR  ++  S   +   Y+           +  R L G    +I+ +QAYI+D++  + R+  MGM+G+ FG+ FIIGP +GG L        V +V+      +FIL +  LPE + ++   + R  G+E  A+ P P      +K N     A   K + +SR                   +++A  +F      +   S   +    L  D Q +G + +F+G+ SV+    ++R L   F +H+L            F WGH++    +  V      SL   G       +   S V P  L            G +LGIS ++ +FA+I+ PF G  L
Sbjct:    4 SPLFILLLTVMLDLIGFGIVLPLLPTYAKDLGASPFMIGLIAAIFSIMQFIFSPLWGKLSDRIGRRPVMLFSIFITAVSYLVFSQTTTIALLIFSRGLSGIGSANIAAAQAYITDVTDSKSRSGAMGMIGAAFGIGFIIGPLIGGLLKHNYGIPMVGYVSAGLIFVDFILAVFFLPESNKNAKKLSFRRSGTEHSADRP-PTSAVLAQKIN---EYAAGVKLAFSSRP---------------LAMLMIANYLFTFAIVNMQVASILLWKEYFLASD-QQIGYIFAFVGLFSVIVQGGMIRNLVKKFGEHKL------------FLWGHLFTFIGVFFVPFAPKESLFTIGLTILFFFAVGTSLVAPINLSLISLYSYKQQQGEILGISQSVNSFARIMGPFSGSIL 378          

BLAST of EWM22466.1 vs. NCBI_GenBank
Match: gi|504902707|ref|WP_015089809.1| (tetracycline resistance MFS efflux pump [Bdellovibrio bacteriovorus] >gi|425859294|gb|AFY00330.1| tetracycline-efflux transporter [Bdellovibrio bacteriovorus str. Tiberius])

HSP 1 Score: 125.561 bits (314), Expect = 1.951e-28
Identity = 107/388 (27.58%), Postives = 179/388 (46.13%), Query Frame = 0

Query:   15 LLLSNALEMLAVALVLPAYPDLTKALGISLQTR-----GLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFIL-LLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQT---LLSTELSTVTPHALVGTVLGISYAIETFAKILTP 393
            + ++  L+M+ + LV+P+ PD+ +   +S +T      G  +S+++L+QFL+SP+LG  SD  GR  +L +S + + F Y+ M  A        GR++ G    +I+V+ AYI+D+S+ + R+ N GM+G+ FGL FIIGPA+GG L    P     VA      NF   L  LPE    S P N+R +   R+ N P     G  R  +      V     +A ++   +W L                                 Y     G     +GL L+ +G++S +   ++ R +   F +H+ +     G A S   +G    LA+   ++ ++ I  ++F T    L + +S  TP    G + G   ++ + A I+TP
Sbjct:   11 IFITVTLDMIGLGLVIPSLPDIMRRF-VSSETSVTEYFGYFISIYALMQFLASPLLGALSDRFGRRSVLLISLLVAGFDYILMAYAPTLEILFAGRIIAGLTGANITVAMAYIADVSNDENRSANFGMVGAAFGLGFIIGPAIGGLLGHLGPEYPFLVAAGLNLLNFFFGLFVLPE----SLPKNMRRKIDLRRTN-PLYSLVGILRSKHLLALLLVYFFFQLAGQTHPSIWTL---------------------------------YTETRYGWTTAQVGLSLAVVGILSAIAQGWLTRLVIPKFGEHRTVVIGSFGYAISFIFFG----LATEGWMMYAILILSAVFWTSPPALQSLISQKTPPQEQGELQGSLVSLSSLAAIITP 355          

BLAST of EWM22466.1 vs. NCBI_GenBank
Match: gi|740666152|ref|WP_038451443.1| (tetracycline resistance MFS efflux pump [Bdellovibrio bacteriovorus] >gi|635303993|gb|AHZ86732.1| tetracycline transporter [Bdellovibrio bacteriovorus])

HSP 1 Score: 125.561 bits (314), Expect = 2.429e-28
Identity = 107/388 (27.58%), Postives = 179/388 (46.13%), Query Frame = 0

Query:   15 LLLSNALEMLAVALVLPAYPDLTKALGISLQTR-----GLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFIL-LLSLPEPSTHSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQT---LLSTELSTVTPHALVGTVLGISYAIETFAKILTP 393
            + ++  L+M+ + LV+P+ PD+ +   +S +T      G  +S+++L+QFL+SP+LG  SD  GR  +L +S + + F Y+ M  A        GR++ G    +I+V+ AYI+D+S+ + R+ N GM+G+ FGL FIIGPA+GG L    P     VA      NF   L  LPE    S P NLR +   R+ N P     G  R  +      V     +A ++   +W L                                 Y     G     +GL L+ +G++S +   ++ R +   F +H+ +     G A S   +G    +A+   ++ ++ I  ++F T    L + +S  TP    G + G   ++ + A I+TP
Sbjct:   11 IFITVTLDMIGLGLVIPSLPDIMRRF-VSSETSVTEYFGYFISIYALMQFLASPLLGALSDRFGRRSVLLISLLVAGFDYILMAYAPTLEILFAGRIIAGLTGANITVAMAYIADVSNDENRSANFGMVGAAFGLGFIIGPAIGGLLGHLGPEYPFLVAAALNLLNFFFGLFILPE----SLPKNLRRKIDLRRTN-PLYSLLGVLRSKHLLALLLVYFFFQLAGQTHPSIWTL---------------------------------YTETRYGWTTAQVGLSLAVVGLLSALAQGWLTRLVIPKFGEHRTVVIGAFGYAVSFIFYG----MATEGWMMYAILILSAVFWTSPPALQSLISHKTPPQEQGELQGSLVSLSSLAAIITP 355          

BLAST of EWM22466.1 vs. NCBI_GenBank
Match: gi|470517166|ref|XP_004353172.1| (transporter, major facilitator subfamily protein [Acanthamoeba castellanii str. Neff] >gi|440802715|gb|ELR23644.1| transporter, major facilitator subfamily protein [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 126.331 bits (316), Expect = 3.085e-28
Identity = 108/392 (27.55%), Postives = 176/392 (44.90%), Query Frame = 0

Query:   11 PLRMLLLSNALEMLAVALVLPAYPDLTKALGISLQTRGLMVSLFSLLQFLSSPILGRGSDVLGRSFMLRVSAVASLFCYVCMLVAKDQRTFMLGRVLPGFFKCSISVSQAYISDISSPQVRAKNMGMMGSMFGLAFIIGPAVGGFLMSKDPWLTVWVALLATAANFIL-----LLSLPEPST----HSAPPNLRGEGSERKANVPNPKEEGSERKANANGTTAVNGKGSVASRSKDGVWDLIRHGPQGGQVAVLLARKVFVAMSSGIFETSFANYASKHLGMDGQTLGLLLSFLGVVSVVNNVFIVRWLSNSFQDHQLIFPAIVGQAFSTFCWGHVYNLASLLPVLASLTITGSIFQTLLSTELSTVTPHALVGTVLGISYAIETFAKILTP 393
            PL  L +   ++MLAV LV+P  P     LG    T G + S++ + Q + SP++G  SD  GR   L VS +AS+  Y  M +A       L R+  G  K ++S+S AY+SD++    RAK +G +G   G+ FIIGPA+GG L      L   VA    +A F+       L LP+ ST             EG E + +V   KE  SE +           +  + +      W L     +   V  L+A      ++   +  +F   +     +D +  G LLS++G++SV      V +L+  F + +L+  A  G   S       +NL  L   L  L +   +  T + + ++   P A +G V+G+  ++++    ++P
Sbjct:   22 PLERLWVLVFVDMLAVGLVIPLLPYYASNLGADAVTYGYLGSIYGISQLIGSPLMGSLSDRYGRVNTLIVSFLASVVSYAMMGMAGSLAMLFLSRIPVGVLKQTMSISYAYVSDVTDSTSRAKYLGFLGVAVGVGFIIGPALGGVLSEVSYTLPALVA----SAMFVFDSVFAYLFLPDGSTIMLDEEKERESGDEGLEGEIDVEKAKESQSEDEVVLPAQAKARQQWDIRAYLN---WVLCVF--KDTSVLFLIATSFAATLAFTTYRVTFPMISQVRFDLDARANGFLLSYMGLLSVFVQGSAVGYLTKRFSEGRLVQLASFGLGVSLCACALAFNLPMLAVALIPLVVCNGVLSTCILSTVTKKVPKADLGGVIGLLGSVQSLCSAVSP 404