EWM22616.1, cds8057 (CDS) Nannochloropsis gaditana

Overview
NameEWM22616.1
Unique Namecds8057
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationKK037247.1:517025..517732 -

Link to JBrowse

Properties
Property NameValue
Protein idEWM22616.1
Productrna methylase
Orig transcript idgnl|cribi|Naga_100528g4.9502.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
KK037247.1supercontigKK037247.1:517025..517732 -
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Molecular Function
TermDefinition
GO:0016740transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0006464cellular protein modification process
Vocabulary: INTERPRO
TermDefinition
IPR026170FAM173
IPR029063SAM-dependent_MTases
Homology
BLAST of EWM22616.1 vs. NCBI_GenBank
Match: gi|585103180|gb|EWM22616.1| (rna methylase [Nannochloropsis gaditana])

HSP 1 Score: 473.781 bits (1218), Expect = 6.768e-169
Identity = 235/235 (100.00%), Postives = 235/235 (100.00%), Query Frame = 0
Query:    1 MHDLETRPCSLAAQIEALVARHVKETEALEDSHLDSCIDEAAIQVQRHTLSPFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDRLVRAWRDFRRVEVVTLTYHLEAPVGGGEGEGVLYRECGGRVEIHTLEKKT 235
            MHDLETRPCSLAAQIEALVARHVKETEALEDSHLDSCIDEAAIQVQRHTLSPFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDRLVRAWRDFRRVEVVTLTYHLEAPVGGGEGEGVLYRECGGRVEIHTLEKKT
Sbjct:    1 MHDLETRPCSLAAQIEALVARHVKETEALEDSHLDSCIDEAAIQVQRHTLSPFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDRLVRAWRDFRRVEVVTLTYHLEAPVGGGEGEGVLYRECGGRVEIHTLEKKT 235          
BLAST of EWM22616.1 vs. NCBI_GenBank
Match: gi|553190538|ref|XP_005855210.1| (rna methylase, partial [Nannochloropsis gaditana CCMP526] >gi|422293850|gb|EKU21150.1| rna methylase, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 457.218 bits (1175), Expect = 1.597e-162
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0
Query:    1 MHDLETRPCSLAAQIEALVARHVKETEALEDSHLDSCIDEAAIQVQRHTLSPFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDRLVRAWRDFRRVEVVTLTYHLEAPVGGGEGEGVLYRECGGRVE 227
            MHDLETRPCSLAAQIEALVARHVKETEALEDSHLDSCIDEAAIQVQRHTLSPFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDRLVRAWRDFRRVEVVTLTYHLEAPVGGGEGEGVLYRECGGRVE
Sbjct:    1 MHDLETRPCSLAAQIEALVARHVKETEALEDSHLDSCIDEAAIQVQRHTLSPFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDRLVRAWRDFRRVEVVTLTYHLEAPVGGGEGEGVLYRECGGRVE 227          
BLAST of EWM22616.1 vs. NCBI_GenBank
Match: gi|219112515|ref|XP_002178009.1| (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >gi|217410894|gb|EEC50823.1| predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 105.916 bits (263), Expect = 4.381e-24
Identity = 61/162 (37.65%), Postives = 86/162 (53.09%), Query Frame = 0
Query:   50 LSPFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDRLVRAWRDFRRVEVVTLTYHLEAPVGG 211
            LSP++PT A  +A       L    +++D+GCGDGR  ++AA L  CR VG+D+S  CIA+A+ +  +E L DR+ +FF  D   D + +L           Q   +E  D  +  V+FLY YPTLL RL  LL +         +  VVTLTYHL   +  
Sbjct:   85 LSPYVPTTAAKIAKFVEFASLLESDVLLDIGCGDGRICVSAARLSGCRAVGIDVSPLCIAMARELAREENLEDRL-SFFEADATIDPKRLL-----------QSPLLE-SDLKDVTVIFLYTYPTLLGRLFPLLAKFTENG----KARVVTLTYHLSQSMAN 229          
BLAST of EWM22616.1 vs. NCBI_GenBank
Match: gi|1209288912|ref|WP_088335618.1| (hypothetical protein [Methanopyrus sp. KOL6])

HSP 1 Score: 72.4034 bits (176), Expect = 3.429e-12
Identity = 50/143 (34.97%), Postives = 72/143 (50.35%), Query Frame = 0
Query:   53 FLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFR----WDLLS-DQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDRLVRAW 190
            F+PT  E +  I   L L P + +VDLGCGDGR +I+AA L  C GVGVD+ +  + LA +     G+ D+ +         DLL  D + V   LV ++  +  E  + C      +V + +  P L A  V  LD L RA+
Sbjct:   36 FVPTPIETVVRILRKLELTPENRVVDLGCGDGRFVISAAYLYGCEGVGVDVREDVLELALTKSETLGVDDKTIFIHSDVRDVDLLELDPDVVFVYLVPSLLEEISEELVSCG---ATIVSYTFEVPGLGAPEVLRLDDLRRAY 175          
BLAST of EWM22616.1 vs. NCBI_GenBank
Match: gi|1129188965|gb|OLP94540.1| (Neurofilament heavy polypeptide [Symbiodinium microadriaticum])

HSP 1 Score: 71.633 bits (174), Expect = 1.167e-10
Identity = 57/167 (34.13%), Postives = 79/167 (47.31%), Query Frame = 0
Query:   49 TLSPFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDRLVRAWRD--FRRVEVVTLTYHLEAPVGGGE 213
            ++SPF+P AA  +  +    RLGP  ++ DLGCGDG  LI AA  C CR VG+DI   CI  A     +  + +R   F R DLL   +  L  L    E ++  AA          +VFL  +      LV L   L   WR+   R++ +VT    L++ V   E
Sbjct: 2129 SVSPFVPCAAGRIPYVLQAARLGPEDVLWDLGCGDGVVLIEAARRCGCRCVGLDIDGPCITAALQRAREANVEER-CTFLRCDLLQLPQGTLGGLSAEQEFQSLPAAT-------CALVFLTAH-----GLVRLSRWLHEEWREGSSRQLRLVTCVESLDSAVDFNE 2282          
BLAST of EWM22616.1 vs. NCBI_GenBank
Match: gi|1150838597|ref|WP_078031418.1| (SAM-dependent methyltransferase [Massilia sp. KIM] >gi|1150050100|gb|OON62762.1| SAM-dependent methyltransferase [Massilia sp. KIM])

HSP 1 Score: 65.0846 bits (157), Expect = 2.330e-9
Identity = 42/134 (31.34%), Postives = 64/134 (47.76%), Query Frame = 0
Query:   46 QRHTLSPFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARL 179
            +R+   P++PT  E +  + ++ ++G   ++ DLGCGDGR ++ AA     RG G+D+    IA AK I  + G+SDR  +F   DL                           D  EA VV LY+ PT+  +L
Sbjct:   38 ERNLDVPYVPTPQEVVDKMLDLAKVGKNDVLYDLGCGDGRIVVTAAKNRGARGTGIDLDPARIAEAKEIAKKAGVSDR-ASFRVGDLFK------------------------TDVSEATVVTLYLLPTVNVKL 146          
BLAST of EWM22616.1 vs. NCBI_GenBank
Match: gi|497242263|ref|WP_009556488.1| (class I SAM-dependent methyltransferase [Oscillatoriales cyanobacterium JSC-12] >gi|410712072|gb|EKQ69578.1| Putative RNA methylase family UPF0020 [Oscillatoriales cyanobacterium JSC-12])

HSP 1 Score: 63.5438 bits (153), Expect = 5.730e-9
Identity = 34/84 (40.48%), Postives = 47/84 (55.95%), Query Frame = 0
Query:   53 FLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQ 136
            FLPT  E +  I  + +L P  ++ DLGCGDGR LIAAA     RGVG+DI +  +  A     Q G+   ++ F++ DL   Q
Sbjct:   10 FLPTPPEVVEGILQLAKLDPNDLVYDLGCGDGRILIAAAQRFGIRGVGIDIDRDRVHEATQSAHQAGVH-HLLTFYQQDLYESQ 92          
BLAST of EWM22616.1 vs. NCBI_GenBank
Match: gi|1125594991|gb|OLE97624.1| (hypothetical protein AUG75_06060 [Cyanobacteria bacterium 13_1_20CM_4_61_6])

HSP 1 Score: 63.5438 bits (153), Expect = 7.605e-9
Identity = 47/134 (35.07%), Postives = 66/134 (49.25%), Query Frame = 0
Query:   52 PFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDR 185
            P+ P+    ++++  + R GP+ ++ DLGCGDGR +IAAA     RGVGV+I    + +A     + G+ DR V F R DL                    EA I      EA VV L++Y TL  RL+  L R
Sbjct:   43 PYFPSTDAAVSAMLELARTGPKDVVYDLGCGDGRIVIAAAKDFGARGVGVEIDPAPLRMAVYRAQRAGVGDR-VRFVRGDLF-------------------EADIG-----EATVVTLFLYETLNRRLLPKLLR 151          
BLAST of EWM22616.1 vs. NCBI_GenBank
Match: gi|1121295482|ref|WP_073607306.1| (class I SAM-dependent methyltransferase [Phormidium tenue] >gi|1115286953|gb|OKH50066.1| SAM-dependent methyltransferase [Phormidium tenue NIES-30])

HSP 1 Score: 62.7734 bits (151), Expect = 7.702e-9
Identity = 41/127 (32.28%), Postives = 59/127 (46.46%), Query Frame = 0
Query:   53 FLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLSDQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARL 179
            F+PT A+ + ++  +  L    ++ DLGCGDGR LI AAL    RGVGVD+    +  A++   QEG+ DR+                           Q+  +   D   A VVF+Y+ P L  RL
Sbjct:   10 FIPTPADAIVAMLKLADLSSSDVVYDLGCGDGRLLIRAALDYGVRGVGVDVDAALLRQAQARAEQEGVGDRLT-------------------------FQQKNLFETDLRNATVVFIYLLPHLNLRL 111          
BLAST of EWM22616.1 vs. NCBI_GenBank
Match: gi|19886829|gb|AAM01668.1| (SAM-dependent methyltransferase [Methanopyrus kandleri AV19])

HSP 1 Score: 63.1586 bits (152), Expect = 1.100e-8
Identity = 52/184 (28.26%), Postives = 85/184 (46.20%), Query Frame = 0
Query:   14 QIEALVARHVKETEA-LEDSHLDSCIDEAAIQVQRHTLSPFLPTAAEDLASIFNILRLGPRHIMVDLGCGDGRPLIAAALLCKCRGVGVDISQQCIALAKSIVSQEGLSDRMVAFFRWDLLS------DQEAVLNRLVEAVEAKAQEAAIECKDGVEAVVVFLYVYPTLLARLVCLLDRLVRAW 190
            ++E L+     E E    D H+ +C  E  +          +PT  E +  I   L L P++ +VDLGCGDGR +I+AA L  C G+GVD+ +  + LA++      + D+ + F   D+        + + V   L+ ++  +  E  + C      VV + +  P L    V  LD L RA+
Sbjct:    6 KVERLLDEFPNEVEYHRSDDHVVTCPTELYL----------VPTPIEIVVEILRKLELTPKNRVVDLGCGDGRFVISAAYLYGCEGMGVDVREDVLELARAKSETLRVDDKTI-FIHSDVRDIDLRELNPDVVFVYLMPSLLEEISEELVSCG---ATVVSYTFEVPGLGVPEVLRLDDLRRAY 175          
The following BLAST results are available for this feature:
BLAST of EWM22616.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585103180|gb|EWM22616.1|6.768e-169100.00rna methylase [Nannochloropsis gaditana][more]
gi|553190538|ref|XP_005855210.1|1.597e-162100.00rna methylase, partial [Nannochloropsis gaditana C... [more]
gi|219112515|ref|XP_002178009.1|4.381e-2437.65predicted protein [Phaeodactylum tricornutum CCAP ... [more]
gi|1209288912|ref|WP_088335618.1|3.429e-1234.97hypothetical protein [Methanopyrus sp. KOL6][more]
gi|1129188965|gb|OLP94540.1|1.167e-1034.13Neurofilament heavy polypeptide [Symbiodinium micr... [more]
gi|1150838597|ref|WP_078031418.1|2.330e-931.34SAM-dependent methyltransferase [Massilia sp. KIM]... [more]
gi|497242263|ref|WP_009556488.1|5.730e-940.48class I SAM-dependent methyltransferase [Oscillato... [more]
gi|1125594991|gb|OLE97624.1|7.605e-935.07hypothetical protein AUG75_06060 [Cyanobacteria ba... [more]
gi|1121295482|ref|WP_073607306.1|7.702e-932.28class I SAM-dependent methyltransferase [Phormidiu... [more]
gi|19886829|gb|AAM01668.1|1.100e-828.26SAM-dependent methyltransferase [Methanopyrus kand... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna8079rna8079Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications