EWM22932.1, cds7742 (CDS) Nannochloropsis gaditana

Overview
NameEWM22932.1
Unique Namecds7742
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002473.1:299247..300128 +

Link to JBrowse

Properties
Property NameValue
Protein idEWM22932.1
Productc-5 sterol desaturase
Orig transcript idgnl|cribi|Naga_100461g3.9044.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002473.1supercontigCM002473.1:299247..300128 +
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Biological Process
TermDefinition
GO:0009987cellular process
GO:0009058biosynthetic process
GO:0006629lipid metabolic process
GO:0006633fatty acid biosynthetic process
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0005488binding
GO:0003824catalytic activity
GO:0005506iron ion binding
GO:0016491oxidoreductase activity
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR006694Fatty_acid_hydroxylase
Homology
BLAST of EWM22932.1 vs. NCBI_GenBank
Match: gi|585103650|gb|EWM22932.1| (c-5 sterol desaturase [Nannochloropsis gaditana])

HSP 1 Score: 593.964 bits (1530), Expect = 0.000e+0
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0
Query:    1 MASTAFLLLESLFLTGENDLFLRGAGGGAFLLWNAHTWLSHHFIAWGLLVLAGLEILACLVFAFGRLRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSKGPALVAWRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFLLPVHALTALAFIVLGGFLASLNHTRFDIKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPYPEEKGAVSGGGGGGGKKE 293
            MASTAFLLLESLFLTGENDLFLRGAGGGAFLLWNAHTWLSHHFIAWGLLVLAGLEILACLVFAFGRLRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSKGPALVAWRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFLLPVHALTALAFIVLGGFLASLNHTRFDIKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPYPEEKGAVSGGGGGGGKKE
Sbjct:    1 MASTAFLLLESLFLTGENDLFLRGAGGGAFLLWNAHTWLSHHFIAWGLLVLAGLEILACLVFAFGRLRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSKGPALVAWRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFLLPVHALTALAFIVLGGFLASLNHTRFDIKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPYPEEKGAVSGGGGGGGKKE 293          
BLAST of EWM22932.1 vs. NCBI_GenBank
Match: gi|298712977|emb|CBJ26879.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 295.819 bits (756), Expect = 1.548e-96
Identity = 152/275 (55.27%), Postives = 193/275 (70.18%), Query Frame = 0
Query:   19 DLFLRGAGGG-AFLLWNA-HTWLSHHFIAWGLLVLAGLEILACLVFAFGRLRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSKGPALVAWRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFLLPVHALTALAFIVLGGFLASLNHTRFDIKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPYP--EEKGAVSGGGGGG 289
            D+ ++GAGGG AF L NA  TW   H+ AW  ++L GLE +A +V  FGR  +  +I  RGKHLD     D  F+AFN+ +T +F+YH +++LW +     VAW V E+   +   AL   +VVYD  YT+FHR LH R LYKHIHKHHHRQKAP+RGN DAVNVHP+EF+ GEYNHL A++LVSR+++PVH ++A AFIV GGF ASLNHTRFDI+ P     +Y+VRYHD+HHW+PE NYGQY MLWD + GS K YP  E++G      GGG
Sbjct:   16 DVLVKGAGGGVAFSLVNAADTWQWWHYPAWLSVILLGLEFVAAMVHVFGRASKAATIRPRGKHLDEFTPLDRLFVAFNRCSTAVFTYHAVQYLWYAPWGGRVAWGVSELGLASGLGALVAQYVVYDLFYTVFHRVLHLRGLYKHIHKHHHRQKAPTRGNADAVNVHPLEFLGGEYNHLLALHLVSRYVVPVHVVSAGAFIVAGGFFASLNHTRFDIRIPF----LYEVRYHDIHHWYPECNYGQYTMLWDWVMGSLKAYPAEEDRGERKRNLGGG 286          
BLAST of EWM22932.1 vs. NCBI_GenBank
Match: gi|922887299|gb|KOO52938.1| (c-5 sterol desaturase [Chrysochromulina sp. CCMP291])

HSP 1 Score: 247.284 bits (630), Expect = 1.734e-78
Identity = 122/236 (51.69%), Postives = 161/236 (68.22%), Query Frame = 0
Query:   47 GLLVLAGLEILACLV-FAFGRLRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSKGPALVAWRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFLLPVHALTALAFIVLGGFLASLNHTRFD---IKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPYPEE 278
            G +   GLE+    V FAF   R  P +P RGKHLD   + D  FI F++LA  + ++H L+F+  S     V W++EE+T  N C+ALPL FV+YD  Y  FHRALHH  +Y  +HKHHHRQ  P+RGNTDA+NVHP EF+ GEYNH+ A+YLVSRF+LPVHAL+ + F+V GG LA+LNHTR D   ++ P    P++ VR HD HH  P SNYGQY MLWD+  G+++ +P++
Sbjct:    2 GAVTQIGLELYRQGVPFAF---RSWPKLPARGKHLDEFASIDRQFIVFSQLAIVVMTFHYLQFMLTSPN---VLWKLEELTVANVCVALPLFFVIYDAFYAPFHRALHHHRVYAFVHKHHHRQVVPTRGNTDAINVHPFEFIMGEYNHVLAVYLVSRFVLPVHALSCVLFLVFGGALATLNHTRLDCNFLRIPFTKVPIFAVRAHDTHHAVPNSNYGQYTMLWDYAMGTYRAHPQD 231          
BLAST of EWM22932.1 vs. NCBI_GenBank
Match: gi|551666303|ref|XP_005835913.1| (hypothetical protein GUITHDRAFT_105560 [Guillardia theta CCMP2712] >gi|428180064|gb|EKX48933.1| hypothetical protein GUITHDRAFT_105560 [Guillardia theta CCMP2712])

HSP 1 Score: 234.958 bits (598), Expect = 1.177e-73
Identity = 127/232 (54.74%), Postives = 157/232 (67.67%), Query Frame = 0
Query:   46 WGLLVLAGLEILACLVFAFGRLRRVPS-IPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSKGPALVAWRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFLLPVHALTALAFIVLGGFLASLNHTRFDIKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPYP 276
            W +++L GLE+++ LV    +    PS IP+RGKHLD L  +D+ FI +NK  TTLF+YH LRF WLS     +AW   EMT  NT +A   LFVVYDF Y ++HR LH R LYK IHKHHH Q APSRGN DAVNVHP+EF+ GEY HL AI     F++P HA+ A  F+VLGG LASLNHTR+DI  P     ++  + HD HH  P  NYGQY MLWD +FG++ P+P
Sbjct:    7 WSVVILLGLELMSYLVNEIPQRFFNPSAIPIRGKHLDVLSWKDISFITWNKFTTTLFTYHALRFCWLSPR---IAWGWSEMTILNTVIAFFSLFVVYDFFYCIWHRILHVRGLYKFIHKHHHHQAAPSRGNADAVNVHPIEFIVGEYCHLLAI-----FIIPCHAVVAAVFVVLGGILASLNHTRYDIAVPL----LFDTKAHDQHHVIPTVNYGQYTMLWDRVFGTYVPHP 226          
BLAST of EWM22932.1 vs. NCBI_GenBank
Match: gi|1210518618|dbj|GAX22494.1| (hypothetical protein FisN_14Hh114 [Fistulifera solaris])

HSP 1 Score: 217.238 bits (552), Expect = 2.526e-65
Identity = 108/234 (46.15%), Postives = 150/234 (64.10%), Query Frame = 0
Query:   47 GLLVLAGLEILACLVFAFGRLRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSKGPALVAWRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFLLPVHALTALAFIVLGGFLASLNHTRFDIKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPY-PEEK 279
            G+ V A  E L  +   +G   +   IP+RGKHLD   ARD+ +I F+K  T  F+Y      +LS       W++ E++  NT LAL  LF+ +DF YTL H ALH   +Y +IHKHHH QKAPSR   DA+NVHP+E++ GEYNHL A++    F++PVH +T++ F+ +GG LA+LNHTR DI    +   +Y  + HDVHH  P+SNYGQY M WD +FG+F+ Y P+++
Sbjct:   63 GISVTAAFEGLDHIAKNWGVWWKSKQIPVRGKHLDYFTARDVIYIGFSKANTAPFTY-----FYLSFAARRCPWKLTEVSATNTLLALIALFIAFDFVYTLLHWALHVPRIYGYIHKHHHHQKAPSRAAVDAINVHPIEYILGEYNHLLALWFCQSFVVPVHGITSVVFLGIGGMLAALNHTRHDIVISFMGVTIYDSKVHDVHHRIPQSNYGQYTMFWDKVFGTFRDYNPKDR 291          
BLAST of EWM22932.1 vs. NCBI_GenBank
Match: gi|1210530658|dbj|GAX10609.1| (hypothetical protein FisN_14Lh114 [Fistulifera solaris])

HSP 1 Score: 208.764 bits (530), Expect = 1.238e-62
Identity = 105/234 (44.87%), Postives = 148/234 (63.25%), Query Frame = 0
Query:   47 GLLVLAGLEILACLVFAFGRLRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSKGPALVAWRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFLLPVHALTALAFIVLGGFLASLNHTRFDIKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPY-PEEK 279
            G+ V A  E L  +   +G   +   IP+RGKHLD   A D+ +I F+K  T  F+Y      +LS       W++ +++  NT +AL  LF+ +DF YTL H ALH   +Y +IHKHHH QKAPSR   DA+NVHP+E++ GEYNHL A++    F++PVH +T++ F+ +GG LA+LNHTR DI    +   +Y  + HDVHH  P+SNYGQY M WD +FG+F+ Y P ++
Sbjct:   10 GISVTAAFEGLDHIAKHWGVWWKSKQIPVRGKHLDYFTAHDVIYIGFSKANTAPFTY-----FYLSFAAHRCPWKLTDVSATNTLIALIALFIAFDFVYTLLHWALHVPRIYGYIHKHHHHQKAPSRAAVDAINVHPIEYILGEYNHLLALWFCHSFVVPVHGITSVVFLGIGGMLAALNHTRHDIVISFMGVTIYDSKGHDVHHRIPQSNYGQYTMFWDKVFGTFRDYNPNDR 238          
BLAST of EWM22932.1 vs. NCBI_GenBank
Match: gi|1072230655|gb|OEU15388.1| (hypothetical protein FRACYDRAFT_218274 [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 208.764 bits (530), Expect = 1.504e-62
Identity = 115/238 (48.32%), Postives = 150/238 (63.03%), Query Frame = 0
Query:   49 LVLAGLEILACLVFAFGR-LRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSKGPALVAWRVEEMT--WQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFL-LPVHALTALAFIVLGGFLASLNHTRFDIKTP--GVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPY-PEEK 279
            L++  LE+L  L    G  +     IP+RGKHLD L ++DL FI  +K  T  F Y LLR+ +       + W   E T   Q   L LP+LF+VYDF YT+ H  LH +A+Y  IHKHHH QKAPSR   DAVNVHPVEF  GEYNHL  I+L  R L + +H L+ L F+ +GG LA  NHTRFDI     G+++ ++  + HDVHH  P+SNYGQY M WD++FGS++PY P ++
Sbjct:   41 LIMLALELLKFLCVLSGESIGSKQKIPVRGKHLDDLSSKDLLFIGISKAQTGPFLYILLRYCFQETN---IQWTFNEDTSILQTVLLPLPVLFIVYDFFYTILHWFLHIKAIYPVIHKHHHHQKAPSRATDDAVNVHPVEFTLGEYNHLWTIFLFCRILNMRMHILSVLLFLAIGGVLAGWNHTRFDITFSIFGIVT-IFDSKAHDVHHRIPQSNYGQYTMFWDNVFGSYRPYDPTDR 274          
BLAST of EWM22932.1 vs. NCBI_GenBank
Match: gi|219121861|ref|XP_002181276.1| (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >gi|217407262|gb|EEC47199.1| predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 203.756 bits (517), Expect = 1.010e-60
Identity = 107/230 (46.52%), Postives = 142/230 (61.74%), Query Frame = 0
Query:   44 IAWGLLVLAGLEILACLVFAFGRLRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSKGPALVAWRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFLLPVHALTALAFIVLGGFLASLNHTRFDIKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFK 273
            + + +L+L   E+L       G   R   IP+RGKHLD L  +D  FI  +K AT  F Y  LRF +       V W +  + + NT   L  LF+VYDF YT+ H ALH +A+Y +IHKHHH QKAPSR N DAVNVHPVEF  GEYNHL  ++L +R +  +H L +L F+ +GGFLA +NHTR+D+        ++  + HDVHH  P+SNYGQY M WD +F S++
Sbjct:   63 VLFAVLILLSFELLDFFSKHSGSWFRSKLIPVRGKHLDELTVKDQLFIGISKAATAPFVYFYLRFAYFEPN---VVWDLANLRFANTVAPLLALFIVYDFFYTILHWALHIKAIYGYIHKHHHHQKAPSRANVDAVNVHPVEFFLGEYNHLWTLFLCTR-VAQIHILGSLLFLAIGGFLAGINHTRYDMVVSFWGVRIFDSKAHDVHHRIPQSNYGQYTMFWDWVFQSYR 288          
BLAST of EWM22932.1 vs. NCBI_GenBank
Match: gi|397640865|gb|EJK74359.1| (hypothetical protein THAOC_03966, partial [Thalassiosira oceanica])

HSP 1 Score: 200.675 bits (509), Expect = 3.007e-58
Identity = 114/272 (41.91%), Postives = 152/272 (55.88%), Query Frame = 0
Query:   54 LEILACLVFAFGRLRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSK----------GPALVA----------------------------WRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFL-------LPVHALTALAFIVLGGFLASLNHTRFDIKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPY-PEEK 279
            LE+++ +V+   R     +IP+RGKHLD    +D  F+ FNK  T LF Y    +LW  +          G  L++                            W ++E++  NT   +P+LFVVYDF YTL H ALH +A+Y  +HKHHH QKAPSR N DAVNVHP+EF  GE+NHL A++LV R +       + V  L AL FI LGG LA LNHTR D         ++  ++HDVHH  P+SNYGQY +LWD IFG+F+ Y P ++
Sbjct:  104 LELVSNIVYYVPRFFSAKTIPVRGKHLDEFSFKDKAFVTFNKCLTGLFVYAYFGYLWSVRKMPGDDEHESGDELLSLQHNHTHVHDHDHTFGNHDHCCGGGKGIWALDELSLANTVFPVPMLFVVYDFFYTLLHWALHVKAVYAFVHKHHHHQKAPSRANIDAVNVHPLEFALGEFNHLLALHLVGRGMPLICYHGMDVSWLGALLFISLGGILAGLNHTRHDAVARVGKYTIFDSKHHDVHHRIPQSNYGQYTVLWDRIFGTFRDYNPNDR 375          
BLAST of EWM22932.1 vs. NCBI_GenBank
Match: gi|397594454|gb|EJK56240.1| (hypothetical protein THAOC_23917 [Thalassiosira oceanica])

HSP 1 Score: 196.823 bits (499), Expect = 3.255e-57
Identity = 112/272 (41.18%), Postives = 151/272 (55.51%), Query Frame = 0
Query:   54 LEILACLVFAFGRLRRVPSIPMRGKHLDTLDARDLGFIAFNKLATTLFSYHLLRFLWLSK----------GPALVA----------------------------WRVEEMTWQNTCLALPLLFVVYDFAYTLFHRALHHRALYKHIHKHHHRQKAPSRGNTDAVNVHPVEFVAGEYNHLAAIYLVSRFL-------LPVHALTALAFIVLGGFLASLNHTRFDIKTPGVLSPVYQVRYHDVHHWFPESNYGQYLMLWDHIFGSFKPY-PEEK 279
            LE+++ +V+   R     +IP+RGKHLD    +D  F+ FNK  T LF Y    +LW  +          G  L++                            W ++E++  NT   +P+LFVVYDF YTL H ALH +A+Y  +HKHHH QKAPSR N DAVNVHP+EF  GE+NH+ A++LV   +       + V  L AL FI LGG LA LNHTR D         ++  ++HDVHH  P+SNYGQY +LWD IFG+F+ Y P ++
Sbjct:   60 LELVSNIVYYVPRFFSAKTIPVRGKHLDEFSFKDKAFVTFNKCLTGLFVYAYFGYLWSVRKMPGDDEHEAGDELLSLQNNHTHVHDHDHTFGNHDHCCGGGKGIWALDELSLANTVFPVPMLFVVYDFFYTLLHWALHVKAVYAFVHKHHHHQKAPSRANIDAVNVHPLEFALGEFNHVLALHLVVGGMPLICYHGMDVSWLGALLFISLGGILAGLNHTRHDAVARVGKYTIFDSKHHDVHHRIPQSNYGQYTVLWDRIFGTFRDYNPNDR 331          
The following BLAST results are available for this feature:
BLAST of EWM22932.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585103650|gb|EWM22932.1|0.000e+0100.00c-5 sterol desaturase [Nannochloropsis gaditana][more]
gi|298712977|emb|CBJ26879.1|1.548e-9655.27conserved unknown protein [Ectocarpus siliculosus][more]
gi|922887299|gb|KOO52938.1|1.734e-7851.69c-5 sterol desaturase [Chrysochromulina sp. CCMP29... [more]
gi|551666303|ref|XP_005835913.1|1.177e-7354.74hypothetical protein GUITHDRAFT_105560 [Guillardia... [more]
gi|1210518618|dbj|GAX22494.1|2.526e-6546.15hypothetical protein FisN_14Hh114 [Fistulifera sol... [more]
gi|1210530658|dbj|GAX10609.1|1.238e-6244.87hypothetical protein FisN_14Lh114 [Fistulifera sol... [more]
gi|1072230655|gb|OEU15388.1|1.504e-6248.32hypothetical protein FRACYDRAFT_218274 [Fragilario... [more]
gi|219121861|ref|XP_002181276.1|1.010e-6046.52predicted protein [Phaeodactylum tricornutum CCAP ... [more]
gi|397640865|gb|EJK74359.1|3.007e-5841.91hypothetical protein THAOC_03966, partial [Thalass... [more]
gi|397594454|gb|EJK56240.1|3.255e-5741.18hypothetical protein THAOC_23917 [Thalassiosira oc... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna7763rna7763Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications