EWM23275.1, cds7398 (CDS) Nannochloropsis gaditana | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

Overview


Name	EWM23275.1
Unique Name	cds7398
Type	CDS
Organism	Nannochloropsis gaditana (N. gaditana B-31)
Alignment location	CM002471.1:544296..544323 -
Alignment location	CM002471.1:544185..544252 -
Alignment location	CM002471.1:543919..544092 -
Alignment location	CM002471.1:543645..543752 -
Alignment location	CM002471.1:543254..543514 -

Link to JBrowse

Properties


Property Name	Value
Protein id	EWM23275.1
Product	Pumilio RNA-binding repeat containing protein
Orig transcript id	gnl\|cribi\|Naga_101027g1.10516.mrna
Gbkey	CDS

Mutants

Expression

No biomaterial libraries express this feature.

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
CM002471.1	supercontig	CM002471.1:544296..544323 -
CM002471.1	supercontig	CM002471.1:544185..544252 -
CM002471.1	supercontig	CM002471.1:543919..544092 -
CM002471.1	supercontig	CM002471.1:543645..543752 -
CM002471.1	supercontig	CM002471.1:543254..543514 -

Analyses

This CDS is derived from or has results from the following analyses


Analysis Name	Date Performed
GO annotation for N. gaditana B31	2020-04-08
BLAST analysis for N. gaditana B-31	2020-04-07
InterPro analysis for N. gaditana B-31	2020-04-06
Gene prediction for N. gaditana B-31	2014-02-18

Annotated Terms

The following terms have been associated with this CDS:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005829	cytosol
GO:0005634	nucleus

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0005488	binding
GO:0003723	RNA binding

Vocabulary: **INTERPRO**
Term	Definition
IPR016024	ARM-type_fold
IPR011989	ARM-like
IPR001313	Pumilio_RNA-bd_rpt

Homology

[Close]

BLAST of EWM23275.1 vs. NCBI_GenBank
Match: gi|585104277|gb|EWM23275.1| (Pumilio RNA-binding repeat containing protein [Nannochloropsis gaditana])

HSP 1 Score: 439.884 bits (1130), Expect = 2.316e-156
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0

Query:    1 MRPRPERQPHHPEYAAFLLPQLEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEKISGEKLV 212
            MRPRPERQPHHPEYAAFLLPQLEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEKISGEKLV
Sbjct:    1 MRPRPERQPHHPEYAAFLLPQLEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEKISGEKLV 212

[Close]

BLAST of EWM23275.1 vs. NCBI_GenBank
Match: gi|298710813|emb|CBJ32228.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 246.899 bits (629), Expect = 8.181e-76
Identity = 120/195 (61.54%), Postives = 149/195 (76.41%), Query Frame = 0

Query:   17 FLLPQLEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEKISGEKL 211
            +L   ++FII+ FVGQ   VEK S H YGCRVIQRILEHC   QK  IL+EI+ S S LIQDQYGNYVIQHV+KHGRP DR  ++ EVK  +L YSQHKFASNVVEKCLQYG+K ER  +I  ++    D +SLLQVMV DPY NYVVQKI+D+AD  Q++ +   +++ A QL+RYT+GKHI+ RLEK+SG+K+
Sbjct:  352 YLHSHIQFIIDGFVGQ---VEKLSMHAYGCRVIQRILEHCIDEQKQVILEEIKDSFSVLIQDQYGNYVIQHVLKHGRPTDRGRLMREVKENLLSYSQHKFASNVVEKCLQYGTKEERGVLIQHLLYGHSDGTSLLQVMVCDPYANYVVQKIIDVADQEQRQTIIMEIKAHAAQLKRYTFGKHIISRLEKLSGKKM 543

HSP 2 Score: 65.4698 bits (158), Expect = 5.773e-9
Identity = 41/141 (29.08%), Postives = 70/141 (49.65%), Query Frame = 0

Query:   30 VGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDI 170
             G G +  +F    +G R IQ+ LE  T   K A  +EI   + +L+ D +GNYV+Q +  +G    R+ + + +    +  S   +   VV+K L+Y S       ID ++  V +    +   V+D  GN+VVQK +++
Sbjct:  208 AGGGGHTVQFCRDQHGSRFIQQKLEVSTDEDKEAFFNEILPHTQSLMTDVFGNYVVQKLFDNGSSAQREALASFLVGHAVQLSLQMYGCRVVQKALEYSS-------IDTLIALVSEFCGQVMKCVQDQNGNHVVQKCIEV 341

[Close]

BLAST of EWM23275.1 vs. NCBI_GenBank
Match: gi|15489333|gb|AAH13765.1| (Pum2 protein, partial [Mus musculus])

HSP 1 Score: 205.297 bits (521), Expect = 9.000e-64
Identity = 102/187 (54.55%), Postives = 135/187 (72.19%), Query Frame = 0

Query:   20 PQ-LEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEK 205
            PQ L+FII+AF GQ   V   STHPYGCRVIQRILEHCT  Q   IL+E+ + +  L+QDQYGNYVIQHV++HGRP D+  +++E++ ++L  SQHKFASNVVEKC+ + S+AER  +IDEV        S L  M++D Y NYVVQK++D+A+  Q++ +   +R     LR+YTYGKHIL +LEK
Sbjct:    1 PQSLQFIIDAFKGQ---VFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEK 184

[Close]

BLAST of EWM23275.1 vs. NCBI_GenBank
Match: gi|1059271643|pdb|5KL1|A (Chain A, Crystal Structure Of The Pumilio-nos-hunchback Rna Complex >gi|1059271647|pdb|5KL8|A Chain A, Crystal Structure Of The Pumilio-nos-cyclinb Rna Complex >gi|1059271650|pdb|5KLA|A Chain A, Crystal Structure Of The Drosophila Pumilio Rna-binding Domain In Complex With Hunchback Rna)

HSP 1 Score: 209.149 bits (531), Expect = 1.712e-63
Identity = 102/184 (55.43%), Postives = 133/184 (72.28%), Query Frame = 0

Query:   22 LEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEK 205
            L+FIINAF GQ   V   STHPYGCRVIQRILEHCT  Q + ILDE+ + +  LIQDQYGNYVIQHV++HG+  D+ ++IN V+ ++LV SQHKFASNVVEKC+ + ++ ER  +IDEV        + L VM++D Y NYVVQK++D+++  Q + L T +R     LR+YTYGKHI  +LEK
Sbjct:  160 LQFIINAFKGQ---VYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTF---NDNALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEK 337

HSP 2 Score: 79.7221 bits (195), Expect = 4.105e-14
Identity = 51/179 (28.49%), Postives = 90/179 (50.28%), Query Frame = 0

Query:   33 GNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVR-LEKISGEK 210
             N++ +FS   +G R IQ+ LE  T A+K  +  EI  ++ +L+ D +GNYVIQ   + G P  +  +  +VK  +L  +   +   V++K L+  S  ++ +I+ E+   VL         V+D  GN+VVQK ++  D    + +    +     L  + YG  ++ R LE  + E+
Sbjct:   24 ANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLK-------CVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQ 195

HSP 3 Score: 73.559 bits (179), Expect = 7.294e-12
Identity = 42/166 (25.30%), Postives = 84/166 (50.60%), Query Frame = 0

Query:   35 NVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHIL 200
            +V + +   YGCRVIQ+ LE  +  Q+  I+ E+       ++DQ GN+V+Q  ++   P   Q +IN  K ++   S H +   V+++ L++ +  +   I+DE+           + +++D YGNYV+Q +L+      +  L   +R     L ++ +  +++
Sbjct:   98 HVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHT-------EQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVV 256

[Close]

BLAST of EWM23275.1 vs. NCBI_GenBank
Match: gi|701305806|ref|XP_010013640.1| (PREDICTED: pumilio homolog 2-like, partial [Nestor notabilis])

HSP 1 Score: 204.912 bits (520), Expect = 3.992e-63
Identity = 102/187 (54.55%), Postives = 135/187 (72.19%), Query Frame = 0

Query:   20 PQ-LEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEK 205
            PQ L+FII+AF GQ   V   STHPYGCRVIQRILEHCT  Q   IL+E+ + +  L+QDQYGNYVIQHV++HGRP D+  +++E++ ++L  SQHKFASNVVEKC+ + S+AER  +IDEV        S L  M++D Y NYVVQK++D+A+  Q++ +   +R     LR+YTYGKHIL +LEK
Sbjct:   27 PQSLQFIIDAFKGQ---VFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEK 210

[Close]

BLAST of EWM23275.1 vs. NCBI_GenBank
Match: gi|704336267|ref|XP_010173746.1| (PREDICTED: pumilio homolog 2-like [Antrostomus carolinensis])

HSP 1 Score: 204.912 bits (520), Expect = 8.302e-63
Identity = 102/187 (54.55%), Postives = 135/187 (72.19%), Query Frame = 0

Query:   20 PQ-LEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEK 205
            PQ L+FII+AF GQ   V   STHPYGCRVIQRILEHCT  Q   IL+E+ + +  L+QDQYGNYVIQHV++HGRP D+  +++E++ ++L  SQHKFASNVVEKC+ + S+AER  +IDEV        S L  M++D Y NYVVQK++D+A+  Q++ +   +R     LR+YTYGKHIL +LEK
Sbjct:   52 PQSLQFIIDAFKGQ---VFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEK 235

HSP 2 Score: 73.9442 bits (180), Expect = 2.214e-12
Identity = 39/157 (24.84%), Postives = 85/157 (54.14%), Query Frame = 0

Query:   44 YGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHIL 200
            YGCRVIQ+ LE  +  Q++ ++ E+       ++DQ GN+V+Q  ++  +P   Q +I+  K ++ V S H +   V+++ L++ +  +   I++E+ +         + +V+D YGNYV+Q +L+      +  + + +R     L ++ +  +++
Sbjct:    2 YGCRVIQKALESISPDQQNEMVKELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHT-------EQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVV 151

[Close]

BLAST of EWM23275.1 vs. NCBI_GenBank
Match: gi|321475178|gb|EFX86141.1| (hypothetical protein DAPPUDRAFT_308458 [Daphnia pulex])

HSP 1 Score: 213.001 bits (541), Expect = 2.062e-62
Identity = 105/187 (56.15%), Postives = 138/187 (73.80%), Query Frame = 0

Query:   21 QLEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKS--SLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEK 205
            QL+FII+AF GQ   V   STHPYGCRVIQRILEHCT  Q S +L+E+  ++ TLIQDQYGNYVIQHV++HG+P D+  ++  VK ++L  SQHKFASNVVEKC+ + +++ER  +IDEV+ S  D S  S L  M++D Y NYVVQK++D+A+  Q++ L   +R     LR+YTYGKHIL +LEK
Sbjct:  378 QLQFIIDAFEGQ---VLSLSTHPYGCRVIQRILEHCTPEQTSPVLNELHHNTETLIQDQYGNYVIQHVLEHGKPEDKLRIVTAVKGKVLSLSQHKFASNVVEKCVSHATRSERASLIDEVI-SFNDASPHSPLHTMMKDQYANYVVQKMIDVAEPSQRKLLMHKIRPHVSTLRKYTYGKHILAKLEK 560

HSP 2 Score: 75.485 bits (184), Expect = 2.308e-12
Identity = 46/170 (27.06%), Postives = 83/170 (48.82%), Query Frame = 0

Query:   34 NNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRL 203
            N++ +FS   +G R IQ+ LE  T A+K  +  EI   +  L+ D +GNYVIQ   + G P  +  +  +++  +L  +   +   V++K L+     ++ +I+ E+   VL         V+D  GN+VVQK ++  D  Q + +          L  + YG  ++ R+
Sbjct:  244 NHIVEFSQDQHGSRFIQQKLERATPAEKQMVFTEIIVGTYALMTDVFGNYVIQKFFEFGSPEQKSTLAQKIRGHVLPLALQMYGCRVIQKALESVPSDQQKEIVRELDGHVLK-------CVKDQNGNHVVQKCIECVDPMQLQFIIDAFEGQVLSLSTHPYGCRVIQRI 406

HSP 3 Score: 71.633 bits (174), Expect = 5.417e-11
Identity = 37/157 (23.57%), Postives = 83/157 (52.87%), Query Frame = 0

Query:   44 YGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHIL 200
            YGCRVIQ+ LE     Q+  I+ E+       ++DQ GN+V+Q  ++   P   Q +I+  + ++L  S H +   V+++ L++ +  + + +++E+  +        + +++D YGNYV+Q +L+      +  + T ++     L ++ +  +++
Sbjct:  326 YGCRVIQKALESVPSDQQKEIVRELDGHVLKCVKDQNGNHVVQKCIECVDPMQLQFIIDAFEGQVLSLSTHPYGCRVIQRILEHCTPEQTSPVLNELHHNT-------ETLIQDQYGNYVIQHVLEHGKPEDKLRIVTAVKGKVLSLSQHKFASNVV 475

[Close]

BLAST of EWM23275.1 vs. NCBI_GenBank
Match: gi|326328009|pdb|3Q0Q|A (Chain A, Crystal Structure Of The Pumilio-homology Domain From Human Pumilio2 In Complex With P38alpha Nrea >gi|326328011|pdb|3Q0R|A Chain A, Crystal Structure Of The Pumilio-homology Domain From Human Pumilio2 In Complex With P38alpha Nreb >gi|326328013|pdb|3Q0S|A Chain A, Crystal Structure Of The Pumilio-homology Domain From Human Pumilio2 In Complex With Erk2 Nre)

HSP 1 Score: 204.912 bits (520), Expect = 1.178e-61
Identity = 102/187 (54.55%), Postives = 135/187 (72.19%), Query Frame = 0

Query:   20 PQ-LEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEK 205
            PQ L+FII+AF GQ   V   STHPYGCRVIQRILEHCT  Q   IL+E+ + +  L+QDQYGNYVIQHV++HGRP D+  +++E++ ++L  SQHKFASNVVEKC+ + S+AER  +IDEV        S L  M++D Y NYVVQK++D+A+  Q++ +   +R     LR+YTYGKHIL +LEK
Sbjct:  158 PQSLQFIIDAFKGQ---VFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEK 341

HSP 2 Score: 71.2478 bits (173), Expect = 5.246e-11
Identity = 40/159 (25.16%), Postives = 85/159 (53.46%), Query Frame = 0

Query:   44 YGCRVIQRILEHCTGAQK--SAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHIL 200
            YGCRVIQ+ LE  +  Q+  S ++ E+       ++DQ GN+V+Q  ++  +P   Q +I+  K ++ V S H +   V+++ L++ +  +   I++E+ +         + +V+D YGNYV+Q +L+      +  + + +R     L ++ +  +++
Sbjct:  106 YGCRVIQKALESISSDQQVISEMVKELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHT-------EQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVV 257

HSP 3 Score: 66.2402 bits (160), Expect = 2.900e-9
Identity = 47/178 (26.40%), Postives = 90/178 (50.56%), Query Frame = 0

Query:   34 NNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVR-LEKISGEK 210
             ++ +FS   +G R IQ+ LE  T A++  + +EI +++  L+ D +GNYVIQ   + G  + +  +   ++  +L  +   +   V++K L+  S +    +I E+V+  LD   L    V+D  GN+VVQK ++    +  + +    +     L  + YG  ++ R LE  + E+
Sbjct:   24 GHIVEFSQDQHGSRFIQQKLERATPAERQMVFNEILQAAYQLMTDVFGNYVIQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALE--SISSDQQVISEMVKE-LDGHVL--KCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQ 196

[Close]

BLAST of EWM23275.1 vs. NCBI_GenBank
Match: gi|699635749|ref|XP_009908929.1| (PREDICTED: pumilio homolog 1-like, partial [Picoides pubescens])

HSP 1 Score: 201.06 bits (510), Expect = 1.197e-61
Identity = 101/187 (54.01%), Postives = 132/187 (70.59%), Query Frame = 0

Query:   20 PQ-LEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEK 205
            PQ L+FII+AF GQ   V   STHPYGCRVIQRILEHC   Q   IL+E+ + +  L+QDQYGNYVIQHV++HGRP D+  ++ E++  +LV SQHKFASNVVEKC+ + S+ ER  +IDEV        S L  M++D Y NYVVQK++D+A+  Q++ +   +R     LR+YTYGKHIL +LEK
Sbjct:   33 PQSLQFIIDAFKGQ---VFALSTHPYGCRVIQRILEHCLPEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAMLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPAQRKIVMHKIRPHIATLRKYTYGKHILAKLEK 216

[Close]

BLAST of EWM23275.1 vs. NCBI_GenBank
Match: gi|242556581|pdb|3GVO|A (Chain A, Structure And Rna Binding Of The Mouse Pumilio-2 Puf Domain >gi|242556583|pdb|3GVT|A Chain A, Structure And Rna Binding Of The Mouse Pumilio-2 Puf Domain >gi|242556584|pdb|3GVT|B Chain B, Structure And Rna Binding Of The Mouse Pumilio-2 Puf Domain)

HSP 1 Score: 204.912 bits (520), Expect = 1.355e-61
Identity = 102/187 (54.55%), Postives = 135/187 (72.19%), Query Frame = 0

Query:   20 PQ-LEFIINAFVGQGNNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVRLEK 205
            PQ L+FII+AF GQ   V   STHPYGCRVIQRILEHCT  Q   IL+E+ + +  L+QDQYGNYVIQHV++HGRP D+  +++E++ ++L  SQHKFASNVVEKC+ + S+AER  +IDEV        S L  M++D Y NYVVQK++D+A+  Q++ +   +R     LR+YTYGKHIL +LEK
Sbjct:  158 PQSLQFIIDAFKGQ---VFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEK 341

HSP 2 Score: 75.8702 bits (185), Expect = 9.172e-13
Identity = 40/157 (25.48%), Postives = 85/157 (54.14%), Query Frame = 0

Query:   44 YGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHIL 200
            YGCRVIQ+ LE  +  Q+S ++ E+       ++DQ GN+V+Q  ++  +P   Q +I+  K ++ V S H +   V+++ L++ +  +   I++E+ +         + +V+D YGNYV+Q +L+      +  + + +R     L ++ +  +++
Sbjct:  108 YGCRVIQKALESISSDQQSEMVKELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHT-------EQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVV 257

HSP 3 Score: 67.781 bits (164), Expect = 7.529e-10
Identity = 44/178 (24.72%), Postives = 90/178 (50.56%), Query Frame = 0

Query:   34 NNVEKFSTHPYGCRVIQRILEHCTGAQKSAILDEIRKSSSTLIQDQYGNYVIQHVMKHGRPNDRQMVINEVKSRMLVYSQHKFASNVVEKCLQYGSKAERNDIIDEVVRSVLDKSSLLQVMVRDPYGNYVVQKILDIADDRQQEALCTYLRSCAPQLRRYTYGKHILVR-LEKISGEK 210
             ++ +FS   +G R IQ+ LE  T A++  + +EI +++  L+ D +GNYVIQ   + G  + +  +   ++  +L  +   +   V++K L+  S  ++++++ E+   VL         V+D  GN+VVQK ++    +  + +    +     L  + YG  ++ R LE  + E+
Sbjct:   26 GHIVEFSQDQHGSRFIQQKLERATPAERQIVFNEILQAAYQLMTDVFGNYVIQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVLK-------CVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQ 196

The following BLAST results are available for this feature:

BLAST of EWM23275.1 vs. NCBI_GenBank

BLAST of EWM23275.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10


Match Name	E-value	Identity	Description
gi\|585104277\|gb\|EWM23275.1\|	2.316e-156	100.00	Pumilio RNA-binding repeat containing protein [Nan...	[more]
gi\|298710813\|emb\|CBJ32228.1\|	8.181e-76	61.54	conserved unknown protein [Ectocarpus siliculosus]	[more]
gi\|15489333\|gb\|AAH13765.1\|	9.000e-64	54.55	Pum2 protein, partial [Mus musculus]	[more]
gi\|1059271643\|pdb\|5KL1\|A	1.712e-63	55.43	Chain A, Crystal Structure Of The Pumilio-nos-hunc...	[more]
gi\|701305806\|ref\|XP_010013640.1\|	3.992e-63	54.55	PREDICTED: pumilio homolog 2-like, partial [Nestor...	[more]
gi\|704336267\|ref\|XP_010173746.1\|	8.302e-63	54.55	PREDICTED: pumilio homolog 2-like [Antrostomus car...	[more]
gi\|321475178\|gb\|EFX86141.1\|	2.062e-62	56.15	hypothetical protein DAPPUDRAFT_308458 [Daphnia pu...	[more]
gi\|326328009\|pdb\|3Q0Q\|A	1.178e-61	54.55	Chain A, Crystal Structure Of The Pumilio-homology...	[more]
gi\|699635749\|ref\|XP_009908929.1\|	1.197e-61	54.01	PREDICTED: pumilio homolog 1-like, partial [Picoid...	[more]
gi\|242556581\|pdb\|3GVO\|A	1.355e-61	54.55	Chain A, Structure And Rna Binding Of The Mouse Pu...	[more]

Relationships

This CDS is a part of the following mRNA feature(s):


Feature Name	Unique Name	Species	Type
rna7415	rna7415	Nannochloropsis gaditana (N. gaditana B-31)	mRNA

Sequences

Synonyms

Publications