EWM23300.1, cds7373 (CDS) Nannochloropsis gaditana

BLAST of EWM23300.1 vs. NCBI_GenBank
Match: gi|585104306|gb|EWM23300.1| (amp-dependent synthetase and ligase, partial [Nannochloropsis gaditana])

HSP 1 Score: 1076.23 bits (2782), Expect = 0.000e+0
Identity = 532/532 (100.00%), Postives = 532/532 (100.00%), Query Frame = 0

Query:    1 TFLPLSLPPSLLPLAELANLPPPTQVLTFDEALSASLARPLTFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLADIARAMGKSFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMYR 532
            TFLPLSLPPSLLPLAELANLPPPTQVLTFDEALSASLARPLTFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLADIARAMGKSFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMYR
Sbjct:    1 TFLPLSLPPSLLPLAELANLPPPTQVLTFDEALSASLARPLTFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLADIARAMGKSFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMYR 532          

BLAST of EWM23300.1 vs. NCBI_GenBank
Match: gi|298711624|emb|CBJ32681.1| (long chain acyl-CoA synthetase [Ectocarpus siliculosus])

HSP 1 Score: 466.848 bits (1200), Expect = 2.996e-154
Identity = 260/519 (50.10%), Postives = 351/519 (67.63%), Query Frame = 0

Query:   14 LAELANLPPPTQVLTFDEALSASLARPLTFRPVPKDVRSV-ATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLADIARAMGKSFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMY 531
            L E  N+P   +V+TF E L       L   PV +  RS+ ATLVYTSGTT KPKG VL H+NLLHQV+ NSF D     P  NP +GDV VS+LPCWHIFERTAEY+  ++G+ + YSNV+NFK+DL K +P F++ VPRLLETI++GV  +  A+    +K     TRV S  +KA R   G V+R   P   E+ L+ VL  V  PL    +KLVW K+R G+GGR+KV+V+GGSS+P  LEDFFE+    V+VGYG+TETSPVI NRVA +N  G+ G+    +EVKI D E+G ++  GQ G +L+RGPQ+M+GY++N EA+ AV+D++G+ DTGDLGR +P+T  LI+TGRAKDTIVLSNGENVEPQP+ED +    +L+DQVM  GQDE+ LG +V  N   +  AG++ +  A R + LLG   LT G AG+  EL + E  +     +++A++ D  +A+    R  E+VG+V +  EPF  +NG +TQTLK KRNVV+  +++EI+ +Y
Sbjct:  210 LQESLNIPESIKVVTFSECLDKGGV--LDAAPVVE--RSMKATLVYTSGTTGKPKGAVLTHANLLHQVSQNSF-DRSEGSP-MNPWVGDVFVSILPCWHIFERTAEYFTLTRGVTMGYSNVRNFKSDLKKFKPHFLIVVPRLLETIWKGVQTQLEAKSKGAQKAAGALTRVSSLRMKAARRFSGTVIRDSKPKGPEKALSAVLMGVTLPLKLAAEKLVWGKIRDGVGGRVKVVVSGGSSLPSFLEDFFEMAGVRVLVGYGLTETSPVIANRVATENTKGTTGKPVPGSEVKIADQETGQQVAVGQTGKILIRGPQVMSGYQNNPEATAAVIDKDGYFDTGDLGRFNPITGDLIVTGRAKDTIVLSNGENVEPQPLEDAISGACSLIDQVMLFGQDERFLGAIVCLNPLNMGAAGIISQEDASRYSGLLGPTPLTTGPAGTPKELADAEKEILGAPGLERAVM-DQLKAVAGEERPWEQVGSVIMKFEPFTTSNGLITQTLKTKRNVVAERFSKEIDALY 721          

BLAST of EWM23300.1 vs. NCBI_GenBank
Match: gi|515862160|ref|WP_017292788.1| (long-chain fatty acid--CoA ligase [Geminocystis herdmanii])

HSP 1 Score: 433.721 bits (1114), Expect = 2.036e-142
Identity = 226/531 (42.56%), Postives = 330/531 (62.15%), Query Frame = 0

Query:   16 ELANLPPPTQVLTFDEALSASLARPL-------------TFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLADIARAMGK--SFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMY 531
            EL      + VL  DEA+    A+P+             TF PV +D+  +ATL+YTSGTT +PKGV+L H NLLHQV Y         E    P+ GD ++S+LP WH +ER AEY++ S+G  ++Y+N++ FK DL +++P F+V VPRL E+IY GV ++F      ++KI+ FF  V   ++ A R+A+GL L +  P+ IE++ A + AL+L PL  +GDKLV+ K+R G+GG +K  ++GG S+   +++FF+++  P+IVGYG+TETSPV   R   +N+ G+ G+   +TE+KI+D E+   LP G+ GLV ++G Q+M GY  N EA+   +D EG+ D+GDLG + P    L+ITGRAKDTIVLSNGEN+EPQP+ED  C  S  +DQ+M VGQD+K LG L+VPN+ AL +                  Q L      + A ++ +E S    KEV      ++AR +     +R  +R+GA +++LEPF+M NG +TQTLK+KR V++ HY   I  M+
Sbjct:  131 ELKEFSLKSIVLLSDEAIETEFAQPIYNFQQFMELGANNTFTPVSQDIDDLATLIYTSGTTGQPKGVMLSHRNLLHQVRY--------LETVIKPLPGDRILSILPSWHSYERAAEYFLLSRGTTLIYTNIRYFKGDLKEYRPHFMVGVPRLWESIYEGVQKQFRDGSKNQQKIVSFFLSVSQKYLDATRIAQGLTLDNLNPSSIEKITAQITALLLLPLHKLGDKLVYQKIRDGVGGCVKTWISGGGSLAKHIDNFFQIVGIPLIVGYGLTETSPVTNARRLHRNIVGASGQPITETEIKIIDLETKKTLPVGKIGLVCIKGTQVMQGYYKNPEATAKAIDSEGWFDSGDLGMVTP-QNDLVITGRAKDTIVLSNGENIEPQPLED-ACVRSPYIDQIMVVGQDQKYLGALIVPNLDALQKWA--------------QSQNLNLTFPDASATMDMIENSDLYAKEVLSLYKQELAREVKNRAGYRPDDRIGAFKLILEPFSMDNGMMTQTLKIKRPVITSHYQDIINSMF 637          

BLAST of EWM23300.1 vs. NCBI_GenBank
Match: gi|1053984123|ref|WP_066121074.1| (long-chain fatty acid--CoA ligase [Geminocystis sp. NIES-3709] >gi|770473648|dbj|BAQ66540.1| long-chain-fatty-acid--CoA ligase [Geminocystis sp. NIES-3709])

HSP 1 Score: 431.024 bits (1107), Expect = 2.901e-141
Identity = 217/531 (40.87%), Postives = 329/531 (61.96%), Query Frame = 0

Query:   16 ELANLPPPTQVLTFDEALSASLARPL-------------TFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLADIARAMGK--SFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMY 531
            EL+     + +L  DEA+    ++P+             TF PV K++  +ATL+YTSGTT +PKGV+L H NLLHQV Y         E    P+ GD ++S+LP WH +ER AEY++ S+G  ++Y+N++ FKADL +++P F+V VPRL E+IY G+ ++F      ++K++ FF  +   +++A R+++GL L +  P+P+E++ A V AL+  PL  +GDKLV+ KVR G+G  +K  ++GG S+P  +++FF+++   +IVGYG+TETSPV   R  ++N+ G+ G+   +TE+KI+D E+   LP+G+ GLV +RG Q+M GY  N  A+   +D +G+ D+GDLG + P    L+ITGRAKDTIVLSNGEN+EPQP+ED  C  S  +DQ+M VGQD+K LG L+VPN+ AL +                  Q L+       A+L  +E S    KEV      ++ R +     +R  +R+   + ++EPF+M NG +TQTLK+KR V++ HY   I  M+
Sbjct:  132 ELSQFNLKSIILLSDEAIETEFSQPIYNFEQFMVLGKNHTFTPVSKNLDDLATLIYTSGTTGQPKGVMLSHGNLLHQVRY--------LETVIKPLPGDRILSILPSWHSYERAAEYFLLSRGTTLIYTNIRYFKADLKEYRPHFMVGVPRLWESIYEGIQKQFRDGSKTQQKLVFFFLSMSEKYLEATRISKGLTLDNLNPSPLEKITAQVTALLFLPLHKLGDKLVYQKVRDGVGSCVKTWISGGGSLPKHIDNFFQIVGISLIVGYGLTETSPVTNARRFDRNIVGASGQPIPETEIKIIDLETKETLPQGKIGLVYIRGSQVMQGYYKNPSATAKAIDSDGWFDSGDLGWVTP-ENDLVITGRAKDTIVLSNGENIEPQPLED-ACVRSPYIDQIMVVGQDQKYLGALIVPNLDALRK--------------WTQSQNLSLTFPDVSADLSMIENSDLYAKEVISLYKQELNREVKNRAGYRPDDRISVFKFIVEPFSMDNGMMTQTLKIKRPVITSHYQDMINSMF 638          

BLAST of EWM23300.1 vs. NCBI_GenBank
Match: gi|1003387106|dbj|BAU66930.1| (AMP-dependent synthetase and ligase [Stanieria sp. NIES-3757])

HSP 1 Score: 430.639 bits (1106), Expect = 3.055e-141
Identity = 225/532 (42.29%), Postives = 327/532 (61.47%), Query Frame = 0

Query:   15 AELANLPPPTQVLTFDEALSASLARPL-------------TFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLADIARAMGK--SFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMY 531
            + +A+LP    VL  DE   + LA  +             T + +P+   ++ATL+YTSGTT KPKG +L H NLLHQVN  + T     +      +GD ++S+LP WH +ER+AEY++F++G  + Y+N+++ K DL K+QP ++VAVPRL E+IY GV ++F  +   K+K+I FF  +   +++A R+A+ + L    P+  ERLLA   A +LSPL  + DK+V+ KVR  +G  +K LV GG S+   L+DF+E+++ P++VGYG+TETSPV   R  + N  G+ G+    TE+KIVDP+S   LP+G+ GLVL+RGPQ+M GY  N EA++  +D EG+ D+GDLG + P    LI+TGRAKDTIVLSNGEN+EPQPIED  C  S  +DQ+M VGQD++ LG L+VPN+ AL +                  Q L   +    A L E+  S    K V+     ++   +     +R  +R+G+  ++LEPF+  NG +TQTLK+KR VV   Y Q I+ M+
Sbjct:  130 SRVADLPIELIVLLSDETPESDLAIKIVNFQQLMATGSNNTLQAIPQKKENLATLIYTSGTTGKPKGAMLTHGNLLHQVN--NLTSIIKAD------VGDRVLSILPSWHAYERSAEYFLFAQGCTLYYTNLRSLKNDLQKYQPHYMVAVPRLWESIYEGVQKQFREQPPNKQKLINFFFEISQEYIEARRIAQKMSLEHLNPSASERLLAQTKASLLSPLYNLADKIVFKKVRQAVGSNVKTLVNGGGSLAKHLDDFYEIVKIPLLVGYGLTETSPVTNARTLKHNFRGTAGKPIPQTEIKIVDPQSRQTLPQGEQGLVLIRGPQVMQGYYKNPEATQKAIDPEGWFDSGDLGWVTP-DNDLILTGRAKDTIVLSNGENIEPQPIED-ACVRSPYIDQIMLVGQDQRALGALIVPNLDALQQWAT--------------NQKLNLNLPNPNASLAEISQSDLYSKPVQNLFRQELNSQVQNRPGYRADDRIGSFRLILEPFSQENGMMTQTLKIKRPVVQQRYQQLIDSMF 637          

BLAST of EWM23300.1 vs. NCBI_GenBank
Match: gi|1054577213|ref|WP_066346030.1| (long-chain fatty acid--CoA ligase [Geminocystis sp. NIES-3708] >gi|770468673|dbj|BAQ61571.1| Long-chain-fatty-acid--CoA ligase [Geminocystis sp. NIES-3708])

HSP 1 Score: 428.713 bits (1101), Expect = 2.183e-140
Identity = 218/532 (40.98%), Postives = 325/532 (61.09%), Query Frame = 0

Query:   15 AELANLPPPTQVLTFDEALSASLARPL-------------TFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLADIARAMGK--SFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMY 531
            +EL      + +L  DEA+    ++P+             +F PV K++  +ATL+YTSGTT KPKGV+L H NLLHQV Y         E    P+ GD ++S+LP WH +ER AEY++ S+G  ++Y+N++ FK DL +++P F+V VPRL E+IY GV ++F      ++K++ FF  +   +++A R+ +GL L    P  +E+++A + AL L PL  +GDKLV+ K+R G+GG +K  ++GG S+   +++FF+++  P+IVGYG+TETSPV   R   +N+ G+ G+   +TE+KI+D ++ A LP+G+ GLV +RG Q+M GY  N EA+   +D +G+ D+GDLG + P    L+ITGRAKDTIVLSNGEN+EPQP+ED  C  S  +DQ+M VGQD+K LG L+VPN+ AL +                  Q L        A  EE+E S    KEV      ++ R +     +R  +R+    ++LE F+M NG +TQTLK+KR V++ HY   I  M+
Sbjct:  131 SELKEFNLKSIILLSDEAIETEFSQPIYNFEQFMALGDSHSFTPVSKNLDDLATLIYTSGTTGKPKGVMLSHGNLLHQVRY--------LETVIKPLPGDRILSILPSWHSYERAAEYFLLSRGTTLIYTNIRYFKGDLKEYRPHFMVGVPRLWESIYEGVQKQFRDGSKTQQKLVFFFLSLSEKYLEATRIYKGLTLEKLNPTFLEKIIAQITALSLFPLHKLGDKLVYQKIRDGVGGCVKTWISGGGSLAKHIDNFFQIVGIPLIVGYGLTETSPVTNARRVTRNIVGASGQPIPETEIKIIDLDTKATLPQGEIGLVCIRGSQVMQGYYKNPEATAKAIDSQGWFDSGDLGMVTP-ENDLVITGRAKDTIVLSNGENIEPQPLED-ACIRSPYIDQIMVVGQDQKYLGALIVPNIDALQKWA--------------QSQNLNLTFPDVSASPEELENSDLYAKEVISLYKQELNREVKNRAGYRPDDRIAIFNLILESFSMDNGMMTQTLKIKRPVITSHYQDMINSMF 638          

BLAST of EWM23300.1 vs. NCBI_GenBank
Match: gi|754511980|ref|WP_041922643.1| (long-chain fatty acid--CoA ligase [Cyanobacterium aponinum])

HSP 1 Score: 422.935 bits (1086), Expect = 3.426e-138
Identity = 219/517 (42.36%), Postives = 321/517 (62.09%), Query Frame = 0

Query:   16 ELANLPPPTQVLTFDEALSASLARPLTFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLA-DIARAMGKSFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMY 531
            E  N   P     F + L     RP  F PV KD  ++ATL+YTSGTT KPKGV+L H NLLHQV +      P K        GD ++S+LP WH +ER AEY++ S+G  ++Y+N++ FK DL K +P  ++ VPRL E+IY GV ++F      ++K++ FF      ++KA R+  GL L    P+  ++L A + + +L P+  +GDKL+++KVR G+GG I+  V+GG S+   +++FF+++  P+IVGYG+TETSPV   R   +N+ G+ G+   +TE+KIV+PE+   LP+G+ GLV +RG Q+M GY  N EA+   +D+EG+ D+GDLG + P    L+ITGRAKDTIVLSNGEN+EPQP+ED  C  S  +DQ+M VGQD+K LG L+VPN+ AL + G       E    +L        +  +RAE+E+ +        + K  LA ++    G  +R  +R+   E++LEPF++ NG +TQTLK+KR V++ HY   I  M+
Sbjct:  147 EEVNRDYPHTTYNFSQFLQLGAQRPFIFVPVEKD--TLATLIYTSGTTGKPKGVMLSHRNLLHQVCHLDVILQPKK--------GDRILSILPSWHSYERAAEYFLLSRGTTLIYTNIRYFKQDLKKIKPHHMIGVPRLWESIYEGVQKQFRDGSKTQQKLVNFFLATSEKYIKAKRIKNGLDLEHLNPSSGQKLTAKITSTLLLPVHKLGDKLIYTKVREGVGGCIQTWVSGGGSLAKHIDNFFQIVGIPLIVGYGLTETSPVTNARRISRNIVGASGQPIPETEIKIVNPETRETLPQGEKGLVFIRGTQVMQGYYKNPEATTKAIDEEGWFDSGDLGWVTP-DNDLVITGRAKDTIVLSNGENIEPQPLED-ACVRSPYIDQIMVVGQDQKYLGALIVPNLEALQKWG-------EEQNRILN----LPSVGANRAEIEKSDLYSSGVLSLYKQELAREVKNRPG--YRIDDRISVFELILEPFSIDNGMMTQTLKIKRPVITNHYQDMINSMF 638          

BLAST of EWM23300.1 vs. NCBI_GenBank
Match: gi|428683354|gb|AFZ52821.1| (Long-chain-fatty-acid--CoA ligase [Cyanobacterium aponinum PCC 10605])

HSP 1 Score: 422.935 bits (1086), Expect = 3.446e-138
Identity = 219/517 (42.36%), Postives = 321/517 (62.09%), Query Frame = 0

Query:   16 ELANLPPPTQVLTFDEALSASLARPLTFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLA-DIARAMGKSFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMY 531
            E  N   P     F + L     RP  F PV KD  ++ATL+YTSGTT KPKGV+L H NLLHQV +      P K        GD ++S+LP WH +ER AEY++ S+G  ++Y+N++ FK DL K +P  ++ VPRL E+IY GV ++F      ++K++ FF      ++KA R+  GL L    P+  ++L A + + +L P+  +GDKL+++KVR G+GG I+  V+GG S+   +++FF+++  P+IVGYG+TETSPV   R   +N+ G+ G+   +TE+KIV+PE+   LP+G+ GLV +RG Q+M GY  N EA+   +D+EG+ D+GDLG + P    L+ITGRAKDTIVLSNGEN+EPQP+ED  C  S  +DQ+M VGQD+K LG L+VPN+ AL + G       E    +L        +  +RAE+E+ +        + K  LA ++    G  +R  +R+   E++LEPF++ NG +TQTLK+KR V++ HY   I  M+
Sbjct:  149 EEVNRDYPHTTYNFSQFLQLGAQRPFIFVPVEKD--TLATLIYTSGTTGKPKGVMLSHRNLLHQVCHLDVILQPKK--------GDRILSILPSWHSYERAAEYFLLSRGTTLIYTNIRYFKQDLKKIKPHHMIGVPRLWESIYEGVQKQFRDGSKTQQKLVNFFLATSEKYIKAKRIKNGLDLEHLNPSSGQKLTAKITSTLLLPVHKLGDKLIYTKVREGVGGCIQTWVSGGGSLAKHIDNFFQIVGIPLIVGYGLTETSPVTNARRISRNIVGASGQPIPETEIKIVNPETRETLPQGEKGLVFIRGTQVMQGYYKNPEATTKAIDEEGWFDSGDLGWVTP-DNDLVITGRAKDTIVLSNGENIEPQPLED-ACVRSPYIDQIMVVGQDQKYLGALIVPNLEALQKWG-------EEQNRILN----LPSVGANRAEIEKSDLYSSGVLSLYKQELAREVKNRPG--YRIDDRISVFELILEPFSIDNGMMTQTLKIKRPVITNHYQDMINSMF 640          

BLAST of EWM23300.1 vs. NCBI_GenBank
Match: gi|505007533|ref|WP_015194635.1| (long-chain fatty acid--CoA ligase [Stanieria cyanosphaera] >gi|428271032|gb|AFZ36973.1| Long-chain-fatty-acid--CoA ligase [Stanieria cyanosphaera PCC 7437])

HSP 1 Score: 421.394 bits (1082), Expect = 1.571e-137
Identity = 214/509 (42.04%), Postives = 319/509 (62.67%), Query Frame = 0

Query:   25 QVLTFDEALSASLARPLTFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLADIARAMGK--SFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMY 531
            Q++ F++ +     +  + +PV +   ++ATL+YTSGTT KPKG +L H NLLHQVN  + T     +      +GD ++S+LP WH +ER+AEY++F++G  + Y+N+++ K+DL K+QP ++VAVPRL E+IY GV ++F  +  +K+K+I +F  +   +++A R+A G+ L     +   RLLA   A +L PL  +GD++V+ KVR  +G  +K LV GG S+   L+DF+E+++ P++VGYG+TETSPV   R  + N  G+ G+   +TE+KIVDP++   LP+GQ GLVL+RGPQ+M GY  N EA+   +D EG+ D+GDLG + P    LI+TGRAKDTIVLSNGEN+EPQPIED  C  S  +DQ+M VGQD++ LG L+VPN+ AL +                  Q L   +      L E+  S    K V+     ++   +     +R  +R+G   ++LEPF+  NG +TQTLK+KR VV   Y Q I+ M+
Sbjct:  155 QIVNFEQLMET--GKNHSLQPVQQTKENLATLIYTSGTTGKPKGAMLSHGNLLHQVN--NLTSIIKAD------VGDRVLSILPSWHAYERSAEYFLFAQGCTLYYTNLRSLKSDLQKYQPHYMVAVPRLWESIYEGVQKQFREQPPSKQKLINYFFEIAEEYIEARRIAEGMSLEHLNLSVGARLLAKTKASLLLPLYNLGDRIVFKKVRQAVGSNVKTLVNGGGSLAKHLDDFYEIVKIPLLVGYGLTETSPVTNARTLKHNFRGTAGKPIPETEIKIVDPQTRQTLPQGQQGLVLIRGPQVMQGYYKNPEATAKAIDPEGWFDSGDLGWVTP-DNDLILTGRAKDTIVLSNGENIEPQPIED-ACVRSPYIDQIMLVGQDQRALGALIVPNLDALQQWAT--------------NQKLNLNLPAPNTSLAEISQSDLYSKPVQNLFRQELNSQVQNRPGYRADDRIGTFRLILEPFSQENGTMTQTLKIKRPVVQQRYQQLIDSMF 637          

BLAST of EWM23300.1 vs. NCBI_GenBank
Match: gi|497068385|ref|WP_009456154.1| (MULTISPECIES: long-chain fatty acid--CoA ligase [Fischerella] >gi|353546695|gb|EHC16143.1| Long-chain-fatty-acid--CoA ligase [Fischerella sp. JSC-11])

HSP 1 Score: 421.779 bits (1083), Expect = 1.627e-137
Identity = 215/497 (43.26%), Postives = 313/497 (62.98%), Query Frame = 0

Query:   42 TFRPVPKDVRSVATLVYTSGTTNKPKGVVLRHSNLLHQVNYNSFTDSPSKEPAYNPVLGDVLVSVLPCWHIFERTAEYWMFSKGIHVVYSNVKNFKADLAKHQPQFIVAVPRLLETIYRGVLQKFAAEKGAKKKIIEFFTRVGSAWVKAWRVARGLVLRSRAPNPIERLLALVLALVLSPLAAVGDKLVWSKVRAGLGGRIKVLVAGGSSMPLVLEDFFELLRTPVIVGYGMTETSPVITNRVAEKNLAGSVGRTARDTEVKIVDPESGARLPEGQPGLVLMRGPQMMAGYKSNAEASKAVLDQEGFLDTGDLGRIHPLTKHLIITGRAKDTIVLSNGENVEPQPIEDVVCANSALVDQVMCVGQDEKVLGMLVVPNVRALARAGLVDRGLAERVAELLGGQVLTNGIAGSRAELEEVEASLREKKEVKKALLADIARAMGK-------SFRETERVGAVEVVLEPFNMANGFLTQTLKVKRNVVSGHYAQEIEQMY 531
            + +PV +++  +ATL+YTSGTT KPKGV+L H NL+HQ+        P          GD+++S+LP WH +ER+ EY++ S+G   VY+N+++ K DL ++QP ++VAVPRLLE+IY G  ++F  +  +++++I FF  +G  ++KA R   GL L +  P+  +RL A +    LSPL A+G+KLV+SKVR   GGR+K +++GG ++P  ++DFFE+L   ++VGYG+TETSPV   R   +NL G+ G+    TE KIVDPE+   LP G+ GLVL+RGPQ+M GY  N EA+   +D EG+ D+GDLG + P    LI+TGRAKDTIVL+NGEN+EPQPIED  C  S  +DQ+M VGQD K LG L+VPN+ AL +                      +G      E E V A+  +K  ++  ++ D+ R            +R  +R+G  +++LEPF++ NG +TQTLK+KR+VV  HY   I  M+
Sbjct:  190 SLQPVKRNLSDLATLIYTSGTTGKPKGVMLTHRNLMHQIVAIPVVVQPRP--------GDIVLSILPTWHSYERSCEYFLLSQGCTQVYTNLRSIKRDLKEYQPHYMVAVPRLLESIYEGAQKQFREQPASRQRLINFFFEIGEKYIKARRTLTGLNLENLNPSMGDRLTASLQVAALSPLYALGEKLVYSKVREATGGRVKQMISGGGALPKHVDDFFEILGVEILVGYGLTETSPVTHARRHWRNLRGAAGQPIPGTETKIVDPETKKELPTGERGLVLLRGPQIMQGYYQNPEATAKAIDPEGWFDSGDLGWVTP-QNDLILTGRAKDTIVLTNGENIEPQPIED-ACLRSPYIDQIMLVGQDRKSLGALIVPNLEALEKWA-------------------ASGNLQLTIEDENVTAATSQKINLESKIIQDLFRQELNREVQNRPGYRPDDRIGPFKLILEPFSIENGMMTQTLKIKRHVVMQHYHDIINGMF 657