EWM29943.1, cds730 (CDS) Nannochloropsis gaditana | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

Overview


Name	EWM29943.1
Unique Name	cds730
Type	CDS
Organism	Nannochloropsis gaditana (N. gaditana B-31)
Alignment location	CM002456.1:277461..278481 +

Link to JBrowse

Properties


Property Name	Value
Protein id	EWM29943.1
Product	cl- channel voltage-gated family protein
Partial	true
Orig transcript id	gnl\|cribi\|Naga_100247g12.2326.mrna
Gene	Chloride
Gbkey	CDS
End range	.
End range	278481

Mutants

Expression

No biomaterial libraries express this feature.

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
CM002456.1	supercontig	CM002456.1:277461..278481 +

Analyses

This CDS is derived from or has results from the following analyses


Analysis Name	Date Performed
GO annotation for N. gaditana B31	2020-04-08
BLAST analysis for N. gaditana B-31	2020-04-07
InterPro analysis for N. gaditana B-31	2020-04-06
Gene prediction for N. gaditana B-31	2014-02-18

Annotated Terms

The following terms have been associated with this CDS:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005215	transporter activity
GO:0005216	ion channel activity
GO:0005247	voltage-gated chloride channel activity

Vocabulary: **Biological Process**
Term	Definition
GO:0009987	cellular process
GO:0006810	transport
GO:0006821	chloride transport
GO:0055085	transmembrane transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR014743	Cl-channel_core
IPR001807	Cl-channel_volt-gated

Homology

[Close]

BLAST of EWM29943.1 vs. NCBI_GenBank
Match: gi|585112531|gb|EWM29943.1| (cl- channel voltage-gated family protein, partial [Nannochloropsis gaditana])

HSP 1 Score: 663.685 bits (1711), Expect = 0.000e+0
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0

Query:    1 MAGLPSGRNQEAAGLTMAFSGKQRPRIRSSFPGLCPSPSLPSPTARTLSLLPFGRCIVTTFTILFFSSLSAAFHLSSPPLSLRHHLGLPPFSFSPSLYITGCPRPSPSAHKPRRYLLTRTALSPDHPPRTSSSSPPPSFPITKLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFLTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLSGQAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRLVTLKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEIASAQGGGEGG 340
            MAGLPSGRNQEAAGLTMAFSGKQRPRIRSSFPGLCPSPSLPSPTARTLSLLPFGRCIVTTFTILFFSSLSAAFHLSSPPLSLRHHLGLPPFSFSPSLYITGCPRPSPSAHKPRRYLLTRTALSPDHPPRTSSSSPPPSFPITKLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFLTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLSGQAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRLVTLKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEIASAQGGGEGG
Sbjct:    1 MAGLPSGRNQEAAGLTMAFSGKQRPRIRSSFPGLCPSPSLPSPTARTLSLLPFGRCIVTTFTILFFSSLSAAFHLSSPPLSLRHHLGLPPFSFSPSLYITGCPRPSPSAHKPRRYLLTRTALSPDHPPRTSSSSPPPSFPITKLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFLTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLSGQAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRLVTLKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEIASAQGGGEGG 340

[Close]

BLAST of EWM29943.1 vs. NCBI_GenBank
Match: gi|873222382|emb|CEM31324.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 140.198 bits (352), Expect = 4.412e-33
Identity = 97/188 (51.60%), Postives = 122/188 (64.89%), Query Frame = 0

Query:  157 GLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNF---LTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLSGQAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRLVT--LKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEIASAQGGGEG 339
            G    +A+L  A L G+ TGLS+  FK  I++++  TYG+    +T +L   G       A   +PALGG+ VG++RE    F PGL G   EVD +K    ++ + KS A VATLGTGCSLGPEGPAVELGV  SRLV+     SL++R+QLL AGAAAGVAAGFNAP+AGVFFALEI +A     G
Sbjct:  144 GFAAESAYLSTAILTGVLTGLSVTAFKRSINLVQGLTYGDLAKVVTPSLGILG-------AALLVPALGGLLVGILRELV-GFTPGLKGSLNEVDNRKNCAFQKQLAKSGAGVATLGTGCSLGPEGPAVELGVSCSRLVSQFFSMSLDRRRQLLSAGAAAGVAAGFNAPVAGVFFALEIVTAAKDNVG 323

[Close]

BLAST of EWM29943.1 vs. NCBI_GenBank
Match: gi|298709049|emb|CBJ30998.1| (Chloride channel protein EriC [Ectocarpus siliculosus])

HSP 1 Score: 127.872 bits (320), Expect = 6.793e-29
Identity = 91/200 (45.50%), Postives = 125/200 (62.50%), Query Frame = 0

Query:  143 KLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFL--------TSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAA--REFPPGLSGQAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRLVTL--KASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEI 330
            KL +L + + E        + FL+ AAL+G+ TG ++  FKTLI  ++ ++Y + +        ++ + AF            IP LG + V  +  A   + + PGL G  EEVD+Q P   +R + K+LAAVATLGTG SLGPEGPAVELGV +SR V+   K S+++++ LL AGAAAGVAAGFNAPIAG+FFALEI
Sbjct:  117 KLQKLRQYVFELPEDTAEESRFLIMAALLGVITGTAVTIFKTLIGAVKKFSYDDLIGRVPPGAASTCIGAF------------IPVLGAIGVVSLMWALQLKSYGPGLGGLTEEVDRQVPWRPRRTLGKTLAAVATLGTGNSLGPEGPAVELGVASSRWVSAMSKLSVQRQRMLLGAGAAAGVAAGFNAPIAGIFFALEI 304

[Close]

BLAST of EWM29943.1 vs. NCBI_GenBank
Match: gi|544213481|ref|XP_005536888.1| (probable CLC-type chloride channel [Cyanidioschyzon merolae strain 10D] >gi|449017450|dbj|BAM80852.1| probable CLC-type chloride channel [Cyanidioschyzon merolae strain 10D])

HSP 1 Score: 114.005 bits (284), Expect = 3.595e-24
Identity = 93/268 (34.70%), Postives = 134/268 (50.00%), Query Frame = 0

Query:   88 LPPFSFSPSLYITGCP---RPSPSAHKPRRYLLTRTALSPDHPPRTSSSSPPPSFP------ITKLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFLTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLSGQAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRLV--------TLKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEIASAQGGGE 338
            LPP +F P     G     +P     + +R L  R        P + S +P   FP      +    +  ++L+  D G++     L+ AAL+G  TGL +  FKT I  +  + YG+ +  AL  F      SL + A+P +GG CV  ++        G+      ++K +      A++K++AAV TLG+GCSLGPEGP+VE+G   +RL+          + S E+ +QL   G AAGVAAGFNAP AGV FA EIA   GG  
Sbjct:    2 LPPVAFLPQPVRPGTRSKRKPEARVKRQKRALRRRVPCQSSLTP-SRSQAPKALFPGDVEAWLRAAAKRRQRLQSPDSGVL-----LVLAALVGAGTGLGVTLFKTAIRGVSDFGYGDTVAGALLPFVG----SLNLIAVPTMGGACVSFLKLRFGALDGGIEAMLRSMEKNERFQSAGAIVKAIAAVFTLGSGCSLGPEGPSVEIGASVARLIPQWASERLQWRLSSERLRQLFACGCAAGVAAGFNAPFAGVLFANEIAQRAGGNH 259

[Close]

BLAST of EWM29943.1 vs. NCBI_GenBank
Match: gi|1039174966|gb|OBQ19508.1| (Cl- channel voltage-gated family protein [Anabaena sp. WA113] >gi|1039203430|gb|OBQ44156.1| Cl- channel voltage-gated family protein [Aphanizomenon flos-aquae WA102])

HSP 1 Score: 113.235 bits (282), Expect = 4.505e-24
Identity = 86/208 (41.35%), Postives = 127/208 (61.06%), Query Frame = 0

Query:  129 RTSSSSPPPSFPITKLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFLTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLS---GQAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRLVT--LKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEIA 331
            R S + PPPS    +   L+ + + +     P T  L  A L+G +TG+ I+TF  LI ++  +   NF+   +  +G+       +AA+P LGG+ VG++R   ++F PGLS     ++  + +KPL   R V K +AA  +LG+G SLGPEGP+VE+G     L++  L+ S E+++ LL AGAAAG+AAGFNAPIAGVFFALE+ 
Sbjct:   13 RESPAFPPPSI---QFANLINRFDPS-----PETVVLFLAVLVGGSTGVGIVTFHNLIQLINNFMLENFM-GEIGIWGA-----WTLAAVPTLGGIIVGLMRWRTQDFGPGLSSLIAVSQGKEAKKPL---RPVTKMIAAAVSLGSGASLGPEGPSVEIGTNFGVLLSDILQVSQERQRLLLGAGAAAGLAAGFNAPIAGVFFALEVV 203

[Close]

BLAST of EWM29943.1 vs. NCBI_GenBank
Match: gi|1039180761|gb|OBQ25146.1| (Cl- channel voltage-gated family protein [Aphanizomenon flos-aquae LD13] >gi|1039183374|gb|OBQ27578.1| Cl- channel voltage-gated family protein [Aphanizomenon flos-aquae MDT14a])

HSP 1 Score: 113.235 bits (282), Expect = 4.548e-24
Identity = 86/208 (41.35%), Postives = 127/208 (61.06%), Query Frame = 0

Query:  129 RTSSSSPPPSFPITKLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFLTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLS---GQAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRLVT--LKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEIA 331
            R S + PPPS    +   L+ + + +     P T  L  A L+G +TG+ I+TF  LI ++  +   NF+   +  +G+       +AA+P LGG+ VG++R   ++F PGLS     ++  + +KPL   R V K +AA  +LG+G SLGPEGP+VE+G     L++  L+ S E+++ LL AGAAAG+AAGFNAPIAGVFFALE+ 
Sbjct:   13 RESPAFPPPSI---QFANLINRFDPS-----PETVVLFLAVLVGGSTGVGIVTFHNLIQLINNFMLENFM-GEIGIWGA-----WTLAAVPTLGGIIVGLMRWRTQDFGPGLSSLIAVSQGKEAKKPL---RPVTKMIAAAVSLGSGASLGPEGPSVEIGTNFGVLLSDILQVSQERQRLLLGAGAAAGLAAGFNAPIAGVFFALEVV 203

[Close]

BLAST of EWM29943.1 vs. NCBI_GenBank
Match: gi|515856408|ref|WP_017287036.1| (hypothetical protein [Leptolyngbya boryana] >gi|932878258|dbj|BAS58310.1| Cl- channel voltage-gated family protein [Leptolyngbya boryana IAM M-101] >gi|932884898|dbj|BAS64658.1| Cl- channel voltage-gated family protein [Leptolyngbya boryana dg5] >gi|1210136373|dbj|BAY55478.1| chloride channel protein [Leptolyngbya boryana NIES-2135])

HSP 1 Score: 113.235 bits (282), Expect = 5.735e-24
Identity = 85/208 (40.87%), Postives = 122/208 (58.65%), Query Frame = 0

Query:  133 SSPPPSF--------PITKLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFLTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLSGQAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRLV--TLKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEI 330
            S+PPP          P ++   L+ +L+       P T  L+ A +IG   GL ++ F+ LI +    T  + L   +  FG+       +A +P LGG+ VG++R   +EF PG+SG    V   K +   R ++K++AA  +LGTG SLGPEGP+VE+G     LV  +LK S ++++ LL AGAAAG+AAGFNAPIAGVFFALE+
Sbjct:    3 STPPPKILLPDSSIAPQSRFNGLLNRLQPH-----PDTIVLLLAVVIGGGAGLGVLMFRYLIELFHRLTLED-LMGTIGHFGA-----WTLACVPTLGGILVGLMRWLTKEFSPGISGLIAAVQAGKEMSPTRPIIKTVAAAVSLGTGASLGPEGPSVEVGANFGLLVGQSLKVSRDRQRLLLGAGAAAGLAAGFNAPIAGVFFALEV 199

[Close]

BLAST of EWM29943.1 vs. NCBI_GenBank
Match: gi|648394524|ref|WP_026086275.1| (hypothetical protein [Fischerella muscicola])

HSP 1 Score: 112.849 bits (281), Expect = 6.908e-24
Identity = 88/210 (41.90%), Postives = 122/210 (58.10%), Query Frame = 0

Query:  129 RTSSSSPPPSFPITKLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFLTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLSG-----QAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRL--VTLKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEIA 331
            R  +  P   FP  +LT LV + + +     P T  L+ A LIG  +G+ ++TF  LI ++      N L S +  +G+       +A +P LGG+ VG++R   ++F PGLS      Q  E+ +Q      R V K LAA  +LG+G SLGPEGP+VE+G     L  V L+ S E+++ LL AGAAAG+AAGFNAPIAGVFFALE+ 
Sbjct:   10 RKVTEKPTFGFPSFRLTYLVNRFQPS-----PETVVLLLALLIGSGSGMGVVTFHYLIQLIHHLMLEN-LMSVIGVWGA-----WTLACVPILGGLIVGLMRWRTQDFGPGLSSLIAASQKGEIKRQL-----RPVTKMLAAAVSLGSGASLGPEGPSVEIGANFGMLLSVVLQVSQERQRLLLGAGAAAGLAAGFNAPIAGVFFALEVV 203

[Close]

BLAST of EWM29943.1 vs. NCBI_GenBank
Match: gi|1121231454|ref|WP_073555302.1| (chloride channel protein [Fischerella major] >gi|1115251330|gb|OKH15847.1| chloride channel protein [Fischerella major NIES-592])

HSP 1 Score: 112.464 bits (280), Expect = 9.625e-24
Identity = 88/210 (41.90%), Postives = 122/210 (58.10%), Query Frame = 0

Query:  129 RTSSSSPPPSFPITKLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFLTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLSG-----QAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRL--VTLKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEIA 331
            R  +  P   FP  +LT LV + + +     P T  L+ A LIG  +G+ ++TF  LI ++      N L S +  +G+       +A +P LGG+ VG++R   ++F PGLS      Q  E+ +Q      R V K LAA  +LG+G SLGPEGP+VE+G     L  V L+ S E+++ LL AGAAAG+AAGFNAPIAGVFFALE+ 
Sbjct:   10 RKVTEKPTFGFPSFRLTYLVNRFQPS-----PETVVLLLALLIGSGSGMGVVTFHYLIGLIHHLMLEN-LMSVIGVWGA-----WTLACVPILGGLIVGLMRWRTQDFGPGLSSLIAASQKGEIKRQL-----RPVTKMLAAAVSLGSGASLGPEGPSVEIGANFGMLLSVVLQVSQERQRLLLGAGAAAGLAAGFNAPIAGVFFALEVV 203

[Close]

BLAST of EWM29943.1 vs. NCBI_GenBank
Match: gi|497071325|ref|WP_009457794.1| (MULTISPECIES: hypothetical protein [Fischerella] >gi|353542845|gb|EHC12306.1| Cl- channel voltage-gated family protein [Fischerella sp. JSC-11])

HSP 1 Score: 112.464 bits (280), Expect = 9.716e-24
Identity = 88/210 (41.90%), Postives = 122/210 (58.10%), Query Frame = 0

Query:  129 RTSSSSPPPSFPITKLTRLVEKLEEADIGLVPTTAFLMQAALIGLATGLSIITFKTLISVMETYTYGNFLTSALNAFGSGKPLSLAMAAIPALGGVCVGVIREAAREFPPGLSG-----QAEEVDKQKPLDVKRAVLKSLAAVATLGTGCSLGPEGPAVELGVVASRL--VTLKASLEQRKQLLLAGAAAGVAAGFNAPIAGVFFALEIA 331
            R  +  P   FP  +LT LV + + +     P T  L+ A LIG  +G+ ++TF  LI ++      N L S +  +G+       +A +P LGG+ VG++R   ++F PGLS      Q  E+ +Q      R V K LAA  +LG+G SLGPEGP+VE+G     L  V L+ S E+++ LL AGAAAG+AAGFNAPIAGVFFALE+ 
Sbjct:   10 RKVTEKPTFGFPSFRLTYLVNRFQPS-----PETVVLLLALLIGSGSGMGVVTFHYLIGLIHHLMLEN-LMSVIGVWGA-----WTLACVPILGGLIVGLMRWRTQDFGPGLSSLIAASQKGEIKRQL-----RPVTKMLAAAVSLGSGASLGPEGPSVEIGANFGMLLSVVLQVSQERQRLLLGAGAAAGLAAGFNAPIAGVFFALEVV 203

The following BLAST results are available for this feature:

BLAST of EWM29943.1 vs. NCBI_GenBank

BLAST of EWM29943.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10


Match Name	E-value	Identity	Description
gi\|585112531\|gb\|EWM29943.1\|	0.000e+0	100.00	cl- channel voltage-gated family protein, partial ...	[more]
gi\|873222382\|emb\|CEM31324.1\|	4.412e-33	51.60	unnamed protein product [Vitrella brassicaformis C...	[more]
gi\|298709049\|emb\|CBJ30998.1\|	6.793e-29	45.50	Chloride channel protein EriC [Ectocarpus siliculo...	[more]
gi\|544213481\|ref\|XP_005536888.1\|	3.595e-24	34.70	probable CLC-type chloride channel [Cyanidioschyzo...	[more]
gi\|1039174966\|gb\|OBQ19508.1\|	4.505e-24	41.35	Cl- channel voltage-gated family protein [Anabaena...	[more]
gi\|1039180761\|gb\|OBQ25146.1\|	4.548e-24	41.35	Cl- channel voltage-gated family protein [Aphanizo...	[more]
gi\|515856408\|ref\|WP_017287036.1\|	5.735e-24	40.87	hypothetical protein [Leptolyngbya boryana] >gi\|93...	[more]
gi\|648394524\|ref\|WP_026086275.1\|	6.908e-24	41.90	hypothetical protein [Fischerella muscicola]	[more]
gi\|1121231454\|ref\|WP_073555302.1\|	9.625e-24	41.90	chloride channel protein [Fischerella major] >gi\|1...	[more]
gi\|497071325\|ref\|WP_009457794.1\|	9.716e-24	41.90	MULTISPECIES: hypothetical protein [Fischerella] >...	[more]

Relationships

This CDS is a part of the following mRNA feature(s):


Feature Name	Unique Name	Species	Type
rna730	rna730	Nannochloropsis gaditana (N. gaditana B-31)	mRNA

Sequences

Synonyms

Publications