EWM23640.1, cds7029 (CDS) Nannochloropsis gaditana

BLAST of EWM23640.1 vs. NCBI_GenBank
Match: gi|585104792|gb|EWM23640.1| (n -dimethylguanosine trna methyltransferase [Nannochloropsis gaditana])

HSP 1 Score: 1233.39 bits (3190), Expect = 0.000e+0
Identity = 593/593 (100.00%), Postives = 593/593 (100.00%), Query Frame = 0

Query:    1 MLVPRLLSRRIALFSSATHTNNLPTTVCCWHRDSKCRIFSSVAMEKPVSSNTATNVPEEIPEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKKEGLTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRKRKMEEGGREKEGLEGARETTMKE 593
            MLVPRLLSRRIALFSSATHTNNLPTTVCCWHRDSKCRIFSSVAMEKPVSSNTATNVPEEIPEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKKEGLTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRKRKMEEGGREKEGLEGARETTMKE
Sbjct:    1 MLVPRLLSRRIALFSSATHTNNLPTTVCCWHRDSKCRIFSSVAMEKPVSSNTATNVPEEIPEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKKEGLTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRKRKMEEGGREKEGLEGARETTMKE 593          

BLAST of EWM23640.1 vs. NCBI_GenBank
Match: gi|299115724|emb|CBN74289.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 593.964 bits (1530), Expect = 0.000e+0
Identity = 294/530 (55.47%), Postives = 380/530 (71.70%), Query Frame = 0

Query:   61 PEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKK----EGLTPEQVTAQ----LADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRKRKMEEGGREKEG 582
            P G  L+ EGQA++ F  GNEVFYNKVQ  NRDLSI +++LFAE+RL++K  K L+K    EG    + TAQ    L   DW+ R A++A+ +G+ +LDALAA+ LRSIRY KE+PG+K++VVNDL+E AV+ A RN+ +N+VD +++  QQG+A   M+  + ++++FDVID+DPYGSAAPF+DAAVQ V  GGLLCVTCTD+ VL+G HPE C+ KY +MPTK +Y+HEMA+RIVL ALE QANRYRR++VP+LS+ IDFY+R+FVRVFTS AEVKKA  K AYVHQSLGCGSF+L   GK S +  N S     G     +CP+TG  F++GGPIWS  +H+MEWV   L R ++          G     T +R+ G+LT+VSEEL DVPL Y+L DM  TLHC  PR+ D++AAL+NAG+RVS QHKEP+A KTDAP  V+WD+MRCWV+KHPV  + +K SSP   IL+K P  QA+F+ P  L   +K  +RY  NPE +WGPKS+A G KR  EE     EG
Sbjct:   37 PPGHRLLVEGQAAIPFSEGNEVFYNKVQEFNRDLSIHVIKLFAEKRLQEKTEKALRKTRKREG---AEFTAQDLEGLDATDWSQRAADSASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVQAAQRNIDFNQVDVSKIRVQQGDAISAMHASSAAKQRFDVIDLDPYGSAAPFLDAAVQCVEEGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGKYLHEMALRIVLHALEAQANRYRRHVVPVLSLSIDFYVRLFVRVFTSAAEVKKACTKAAYVHQSLGCGSFFLHPVGKMSNNNYNPSLTTGDG-----LCPQTGKRFKVGGPIWSPAIHNMEWVRLLLDRVQKN--------RGPYQPSTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCNSPRMIDMQAALVNAGFRVSQQHKEPQAVKTDAPDDVVWDVMRCWVKKHPVSDK-RKTSSPGQAILSKDPNHQADFTVPSGLWQ-RKTATRYQQNPEPYWGPKSRATGNKRGAEESCGPSEG 548          

BLAST of EWM23640.1 vs. NCBI_GenBank
Match: gi|970650778|gb|KUF89730.1| (tRNA (guanine(26)-N(2))-dimethyltransferase [Phytophthora nicotianae])

HSP 1 Score: 523.857 bits (1348), Expect = 2.676e-178
Identity = 259/531 (48.78%), Postives = 349/531 (65.73%), Query Frame = 0

Query:   58 EEIPEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKK------EGLTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRKRKMEEGGREKEG 582
            +E P G  +I+EG   M+FP  N+VFYN VQVLNRDLSI M+  FA  R R++  K  +K      EG T   V  +  D +    + +TA E+G+ + +AL+ASGLRSIRYF+++PG+K I+VND++  AVE   RNVA+N +   RV P + +AT VMY H +  +QFDVID+DPYGSA+ F+D+AVQ+VANGGLLCVTCTD+ VL+G  PEVC+ +Y A+P K+ Y+HEMA+R+VLQ +E+ ANRY R+IVP+ S  IDFY+RVFVRV+ SP  VKKA  K++YV+Q + C SF+LQ  G  SG+    +Y AS GP     C +    +++ GPIWSAP+H  + VL+   R E+            +  PT  R+ GLLT +SEELVD PLHYTL  ++  LHC+ PR+  ++ A+ +AGY VS  HK P A KT AP  V+WDIMRCWV+KHP+  +   E +P ++ILAK PK +ANFS        K K  R+P NPE  WGPK++A G +        + EG
Sbjct:   16 KEAPAGHKIIREGNGVMIFPEQNQVFYNPVQVLNRDLSIAMISEFARTRAREQLTKEARKAANAAGEGAT--FVPKEYTDEEIEKHLTDTAEEKGIRIFEALSASGLRSIRYFQQIPGVKSILVNDMDPAAVETIKRNVAFNELPLDRVIPNEADATDVMYNHRKPADQFDVIDLDPYGSASIFLDSAVQSVANGGLLCVTCTDMPVLSGKQPEVCFSRYGALPNKSHYLHEMALRMVLQTIESSANRYARHIVPIASCSIDFYVRVFVRVYKSPVNVKKAMTKLSYVYQCVQCESFHLQPLGVSSGN----TYHASRGPIVGQECDQCKGKYKMAGPIWSAPLHSRDVVLKIRDRVEKST----------SEFPTKPRLHGLLTTISEELVDAPLHYTLPGLSKVLHCSNPRMSQIQGAIRHAGYEVSQFHKIPEAIKTTAPNDVIWDIMRCWVKKHPLNKKRDGEETPGSRILAKEPKFEANFSSKRPGPNDKPKALRFPLNPEPNWGPKARAVGNRNDNVTPASKSEG 530          

BLAST of EWM23640.1 vs. NCBI_GenBank
Match: gi|970657346|gb|KUF95240.1| (Voltage-dependent L-type calcium channel subunit alpha-1S [Phytophthora nicotianae])

HSP 1 Score: 524.242 bits (1349), Expect = 2.749e-178
Identity = 257/519 (49.52%), Postives = 346/519 (66.67%), Query Frame = 0

Query:   58 EEIPEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKK------EGLTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRK 570
            +E P G  +I+EG   M+FP  N+VFYN VQVLNRDLSI M+  FA  R R++  K  +K      EG T   V  +  D +    + +TA E+G+ + +AL+ASGLRSIRYF+++PG+K I+VND++  AVE   RNVA+N +   RV P + +AT VMY H +  +QFDVID+DPYGSA+ F+D+AVQ+VANGGLLCVTCTD+ VL+G  PEVC+ +Y A+P K+ Y+HEMA+R+VLQ +E+ ANRY R+IVP+ S  IDFY+RVFVRV+ SP  VKKA  K++YV+Q + C SF+LQ  G  SG+    +Y AS GP     C +    +++ GPIWSAP+H  + VL+   R E+            +  PT  R+ GLLT +SEELVD PLHYTL  ++  LHC+ PR+  ++ A+ +AGY VS  HK P A KT AP  V+WDIMRCWV+KHP+  +   E +P ++ILAK PK +ANFS        K K  R+P NPE  WGPK++A G +
Sbjct:   16 KEAPAGHKIIREGNGVMIFPEQNQVFYNPVQVLNRDLSIAMISEFARTRAREQLTKEARKAANAAGEGAT--FVPKEYTDEEIEKHLTDTAEEKGIRIFEALSASGLRSIRYFQQIPGVKSILVNDMDPAAVETIKRNVAFNELPLDRVIPNEADATDVMYNHRKPADQFDVIDLDPYGSASIFLDSAVQSVANGGLLCVTCTDMPVLSGKQPEVCFSRYGALPNKSHYLHEMALRMVLQTIESSANRYARHIVPIASCSIDFYVRVFVRVYKSPVNVKKAMTKLSYVYQCVQCESFHLQPLGVSSGN----TYHASRGPIVGQECDQCKGKYKMAGPIWSAPLHSRDVVLKIRDRVEKST----------SEFPTKPRLHGLLTTISEELVDAPLHYTLPGLSKVLHCSNPRMSQIQGAIRHAGYEVSQFHKIPEAIKTTAPNDVIWDIMRCWVKKHPLNKKRDGEETPGSRILAKEPKFEANFSSKRPGPNDKPKALRFPLNPEPNWGPKARAVGNR 518          

BLAST of EWM23640.1 vs. NCBI_GenBank
Match: gi|675192937|ref|XP_008905353.1| (N2,N2-dimethylguanosine tRNA methyltransferase [Phytophthora parasitica INRA-310] >gi|567961582|gb|ETK87122.1| N2,N2-dimethylguanosine tRNA methyltransferase [Phytophthora parasitica] >gi|567990313|gb|ETL40545.1| N2,N2-dimethylguanosine tRNA methyltransferase [Phytophthora parasitica] >gi|568019537|gb|ETL93681.1| N2,N2-dimethylguanosine tRNA methyltransferase [Phytophthora parasitica] >gi|568094744|gb|ETN09520.1| N2,N2-dimethylguanosine tRNA methyltransferase [Phytophthora parasitica INRA-310] >gi|570327902|gb|ETO75850.1| N2,N2-dimethylguanosine tRNA methyltransferase [Phytophthora parasitica P1976] >gi|570951689|gb|ETP16946.1| N2,N2-dimethylguanosine tRNA methyltransferase [Phytophthora parasitica CJ01A1] >gi|570985881|gb|ETP45042.1| N2,N2-dimethylguanosine tRNA methyltransferase [Phytophthora parasitica P10297])

HSP 1 Score: 523.472 bits (1347), Expect = 1.279e-177
Identity = 257/519 (49.52%), Postives = 346/519 (66.67%), Query Frame = 0

Query:   58 EEIPEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKK------EGLTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRK 570
            +E P G  +I+EG   M+FP  N+VFYN VQVLNRDLSI M+  FA  R R++  K  +K      EG T   V  +  D +    + +TA E+G+ + +AL+ASGLRSIRYF+++PG+K I+VND++  AVE   RNVA+N +   RV P + +AT VMY H +  +QFDVID+DPYGSA+ F+D+AVQ+VANGGLLCVTCTD+ VL+G  PEVC+ +Y A+P K+ Y+HEMA+R+VLQ +E+ ANRY R+IVP+ S  IDFY+RVFVRV+ SP  VKKA  K++YV+Q + C SF+LQ  G  SG+    +Y AS GP     C +    +++ GPIWSAP+H  + VL+   R E+            +  PT  R+ GLLT +SEELVD PLHYTL  ++  LHC+ PR+  ++ A+ +AGY VS  HK P A KT AP  V+WDIMRCWV+KHP+  +   E +P ++ILAK PK +ANFS        K K  R+P NPE  WGPK++A G +
Sbjct:   40 KEAPAGHKIIREGNGVMIFPEQNQVFYNPVQVLNRDLSIAMISEFARTRAREQLTKEARKAANAAGEGAT--FVPKEYTDEEIEKHLTDTAEEKGIRIFEALSASGLRSIRYFQQIPGVKSILVNDMDPAAVETIKRNVAFNELPLDRVIPNEADATDVMYNHRKPADQFDVIDLDPYGSASIFLDSAVQSVANGGLLCVTCTDMPVLSGKQPEVCFSRYGALPNKSHYLHEMALRMVLQTIESSANRYARHIVPIASCSIDFYVRVFVRVYKSPVNVKKAMTKLSYVYQCVQCESFHLQPLGVSSGN----TYHASRGPIVGQECDQCKGKYKMAGPIWSAPLHSRDVVLKIRDRVEKST----------SEFPTKPRLHGLLTTISEELVDAPLHYTLPGLSKVLHCSNPRMSQIQGAIRHAGYEVSQFHKIPEAIKTTAPNDVIWDIMRCWVKKHPLNKKRDGEETPGSRILAKEPKFEANFSSKRPGPNDKPKALRFPLNPEPNWGPKARAVGNR 542          

BLAST of EWM23640.1 vs. NCBI_GenBank
Match: gi|566024258|gb|ETI47169.1| (N2,N2-dimethylguanosine tRNA methyltransferase [Phytophthora parasitica P1569] >gi|568049953|gb|ETM46919.1| N2,N2-dimethylguanosine tRNA methyltransferase [Phytophthora parasitica])

HSP 1 Score: 523.087 bits (1346), Expect = 2.276e-177
Identity = 257/519 (49.52%), Postives = 346/519 (66.67%), Query Frame = 0

Query:   58 EEIPEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKK------EGLTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRK 570
            +E P G  +I+EG   M+FP  N+VFYN VQVLNRDLSI M+  FA  R R++  K  +K      EG T   V  +  D +    + +TA E+G+ + +AL+ASGLRSIRYF+++PG+K I+VND++  AVE   RNVA+N +   RV P + +AT VMY H +  +QFDVID+DPYGSA+ F+D+AVQ+VANGGLLCVTCTD+ VL+G  PEVC+ +Y A+P K+ Y+HEMA+R+VLQ +E+ ANRY R+IVP+ S  IDFY+RVFVRV+ SP  VKKA  K++YV+Q + C SF+LQ  G  SG+    +Y AS GP     C +    +++ GPIWSAP+H  + VL+   R E+            +  PT  R+ GLLT +SEELVD PLHYTL  ++  LHC+ PR+  ++ A+ +AGY VS  HK P A KT AP  V+WDIMRCWV+KHP+  +   E +P ++ILAK PK +ANFS        K K  R+P NPE  WGPK++A G +
Sbjct:   40 KEAPAGHKIIREGNGVMIFPEQNQVFYNPVQVLNRDLSIAMISEFARTRAREQLTKEARKAANAAGEGAT--FVPKEYTDEEIEKHLTDTAEEKGIRIFEALSASGLRSIRYFQQIPGVKSILVNDMDPAAVETIKRNVAFNELPLDRVIPNEADATDVMYNHRKPADQFDVIDLDPYGSASIFLDSAVQSVANGGLLCVTCTDMPVLSGKQPEVCFSRYGALPNKSHYLHEMALRMVLQTIESSANRYARHIVPIASCSIDFYVRVFVRVYKSPVNVKKAMTKLSYVYQCVQCESFHLQPLGVSSGN----TYHASRGPIVGQECDQCKGKYKMAGPIWSAPLHSRDVVLKIRDRVEKST----------SEFPTKPRLHGLLTTISEELVDAPLHYTLPGLSKVLHCSNPRMSQIQGAIRHAGYEVSQFHKIPEAIKTTAPNDVIWDIMRCWVKKHPLNKKRDGEETPGSRILAKEPKFEANFSSKRPGPNDKPKALRFPLNPEPNWGPKARAVGNR 542          

BLAST of EWM23640.1 vs. NCBI_GenBank
Match: gi|695406655|ref|XP_009524166.1| (hypothetical protein PHYSODRAFT_488933 [Phytophthora sojae] >gi|348681633|gb|EGZ21449.1| hypothetical protein PHYSODRAFT_488933 [Phytophthora sojae])

HSP 1 Score: 518.464 bits (1334), Expect = 5.786e-176
Identity = 252/514 (49.03%), Postives = 342/514 (66.54%), Query Frame = 0

Query:   61 PEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKKEGLTP----EQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRK 570
            P G  +I+EG   M+FP  N VFYN VQVLNRDLSI M+  FA  R R++ AK  +KE        + V  + +D +    + +TA ++G+ + +AL+ASGLRSIRYF+++PG+K I+VND++  AVE   RNVA+N +   RV P + +AT VMY H +  +QFDVID+DPYGSA+ F+D+AVQ+V+NGGLLCVTCTD+ VL+G  PEVC+ +Y A+P K+ Y+HEMA+R+VLQ +E+ ANRY R+IVP+ S  IDFY+RVFVRV+ SP  VKKA  K++YV+Q + C SF+LQ  G  SG+    SY AS GP     C +    +++ GPIWSAP+H  + VL+   R E+               PT  R+ GLLT +SEELVD PLHYTL  ++  LHC+ PR+  ++ A+ +AGY VS  HK P A KT AP  V+WDIMRCWV+KHP+  +   + +P ++ILA  PK +ANFS        K K  R+P NPE  WGPK++A G +
Sbjct:   18 PAGHKIIREGDGVMIFPEQNRVFYNPVQVLNRDLSIAMISEFARTRAREQLAKESRKEAAAAGKAADFVPKEYSDEEIEQHLKDTAEDKGIRIFEALSASGLRSIRYFQQIPGVKSILVNDMDPAAVETIKRNVAFNELPLDRVIPNEADATDVMYNHRKPADQFDVIDLDPYGSASIFLDSAVQSVSNGGLLCVTCTDMPVLSGKQPEVCFSRYGALPNKSHYLHEMALRMVLQTIESSANRYARHIVPIASCSIDFYVRVFVRVYKSPVNVKKAMTKLSYVYQCVQCESFHLQPLGVSSGN----SYHASRGPIVGQECDQCKGKYKMAGPIWSAPLHSRDVVLKIRERVEKST----------TEFPTKPRLHGLLTTISEELVDAPLHYTLPGLSKVLHCSNPRMSQIQGAIRHAGYEVSQFHKVPEAIKTTAPNDVIWDIMRCWVKKHPLNKKRDGQETPGSRILATKPKFEANFSSKRPGPNEKPKALRFPLNPEPNWGPKARAVGNR 517          

BLAST of EWM23640.1 vs. NCBI_GenBank
Match: gi|301107221|ref|XP_002902693.1| (N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative [Phytophthora infestans T30-4] >gi|262098567|gb|EEY56619.1| N(2),N(2)-dimethylguanosine tRNA methyltransferase, putative [Phytophthora infestans T30-4])

HSP 1 Score: 512.301 bits (1318), Expect = 1.564e-173
Identity = 250/528 (47.35%), Postives = 344/528 (65.15%), Query Frame = 0

Query:   61 PEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKKE----GLTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRKRKMEEGGREKEGLE 584
            P G  +I+EG   M+F   N+VFYN VQVLNRDLSI M+  FA  R R++  +  +K     G +   V  +  D +    +A+TA ++G+ + +AL+ASGLRSIRYF+++PG+K I+VND++  AVE   RNV +N +   RV P + +AT VMY H +  +QFDVID+DPYGSA+ F+D+AVQ+V+NGGLLCVTCTD+ VL+G  PEVC+ +Y A+P K+ Y+HEMA+R+VLQ +E+ ANRY R+IVP+ S  IDFY+RVFVRV+ SP  VKKA  K+++V+Q + C SF+LQ  G  +G+    SY AS GP     C +    +++ GPIWSAP+H  + VL+   R E             +  PT  R+ GLLT +SEELVD PLHYTL  +   LHC+ PR+  ++ A+  AGY VS  HK P A KT AP  V+WDIMRCWV+KHP+  +   E +P ++ILAK PK +ANFS        K K  R+P NPE  WGPK++A G +       + ++ +E
Sbjct:   19 PPGHKIIREGNGVMIFSEQNQVFYNPVQVLNRDLSITMISEFARTRAREQLTREARKAANAAGESASFVPKEYTDEEIEKHLADTAEDKGIRIFEALSASGLRSIRYFQQIPGVKSILVNDMDPAAVENIKRNVVFNELPLDRVIPNEADATDVMYNHRKPADQFDVIDLDPYGSASIFLDSAVQSVSNGGLLCVTCTDMPVLSGKQPEVCFSRYGALPNKSHYLHEMALRMVLQTIESSANRYARHIVPVASCSIDFYVRVFVRVYKSPVNVKKAMTKLSHVYQCVQCESFHLQPLGVSNGN----SYHASRGPIVGQECDQCKGKYKMAGPIWSAPLHSRDVVLKIRDRVE----------MSTSEFPTKPRLHGLLTTISEELVDAPLHYTLPGLCKVLHCSNPRMSQIQGAIRRAGYEVSQFHKVPDAVKTTAPNDVIWDIMRCWVKKHPLNKKRDGEETPGSRILAKEPKFEANFSSKRPGPNDKPKALRFPLNPEPNWGPKARAVGNRVDNAPAAKSEDKVE 532          

BLAST of EWM23640.1 vs. NCBI_GenBank
Match: gi|813133347|ref|XP_012197790.1| (N2,N2-dimethylguanosine tRNA methyltransferase [Saprolegnia parasitica CBS 223.65] >gi|641537330|gb|KDO31183.1| N2,N2-dimethylguanosine tRNA methyltransferase [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 508.064 bits (1307), Expect = 3.214e-172
Identity = 252/522 (48.28%), Postives = 351/522 (67.24%), Query Frame = 0

Query:   50 SNTATNVPEEIPEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRD---KEAKRLKKEGLTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKG 568
            S  AT  P  + +G  +IKEG+A ++FP GN+VFYNKVQVLNRDLSI ++  FA  R R+   K+ KR K E L          D +  A V   A   GL + +AL+ASGLRSIRY++E+ G+K I+VNDL+  AV    RN+ +N +   ++ P + +AT VMY+H +  + FDVID+DPYGSA+ F+D AVQA+ANGGLLCVTCTD+ VL+G  P+VC+ +Y ++P K+ Y+HE A+R+VL ++E+ A RY+++IVP++S  IDFY+RVFVRV+ SP  VK +  K++YV+Q  GC SF++Q  GK++G     +Y AS       +C + G  F++ GPIWS P+HD + VL+           +   K G+A  PT +R+ GLLT+VSEE+VD PL YTL  +  TLHC  PR+D +++A++NAGY+VS  HK P A KTDAP  V+WDIMRCWV+KHP+  + + E     +ILAK PK +A F+      A K+K  R+P NPEA+WGPKS+A+G
Sbjct:    2 STDATKTPTPMADG-EIIKEGRAKIIFPKGNQVFYNKVQVLNRDLSIAVINQFAHERAREAILKQRKREKNEDLP--------TDDEVKAHVLAHAETSGLKIFEALSASGLRSIRYWQEIAGVKSILVNDLDPAAVISIKRNIEFNGLTTDKLIPNEDDATSVMYRHRKEADNFDVIDLDPYGSASIFLDGAVQAIANGGLLCVTCTDMPVLSGKDPDVCFHRYGSVPYKSNYLHENALRMVLHSIESAAIRYQKHIVPIVSCSIDFYVRVFVRVYKSPVNVKASMTKLSYVYQCTGCDSFHMQPLGKFNGK----TYHASFVEPERALCDQCGKKFKMSGPIWSNPLHDRDLVLKI---------RENVIKEGVAKYPTKDRLHGLLTSVSEEVVDAPLFYTLHGICKTLHCGQPRMDQMQSAILNAGYKVSQSHKVPEAIKTDAPNDVIWDIMRCWVKKHPISKKHESEKDAGTQILAKEPKHEAVFTG-KRPSAGKEKALRFPKNPEAYWGPKSRARG 500          

BLAST of EWM23640.1 vs. NCBI_GenBank
Match: gi|325181940|emb|CCA16394.1| (N(2) putative [Albugo laibachii Nc14])

HSP 1 Score: 506.523 bits (1303), Expect = 1.900e-171
Identity = 244/523 (46.65%), Postives = 346/523 (66.16%), Query Frame = 0

Query:   60 IPEGFMLIKEGQASMLFPSGNEVFYNKVQVLNRDLSIMMLRLFAERRLRDKEAKRLKKEGLTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKG-RKRKMEEGGREKE 581
            + E    I+EG+A ++ P  N VFYNKVQVLNRDLSI ++  FA  R R+K     KK+     Q    +   +    +  TA   GL +L+ALAASGLRSIRYF+++PG++ I+VNDL+  AVE   RN+ +N V   RV   + +A+ ++Y H +  EQFDVID+DPYGSAA  +D AVQA++NGGL+CVTCTD+ VL+G +PE C+ +Y ++P KA+Y+HE A+RIVLQA+E  A +Y+R+IVP+ S  IDFY+RVFVRVF SP  VKK+  K+++V Q +GC +F+LQ  G  +G+    ++ A+ GP     C E G+ FR+ GPIWS P+HD E VL             A+    +A  PT +R+ GLLT +SEE+++ PLHYT+  +A TLHCT PR+D  + A+ + GY+VS  HK P A KT APP  +WDIMRCWV KHP+  + + +++P  +ILAK   L+ +F+        K+KV R+P NP+ +WGPK++AKG R   ++E  +E++
Sbjct:   13 LTENMEKIQEGRAVIITPEQNRVFYNKVQVLNRDLSIAVISDFARERAREKMLLERKKKQDGNAQADEHIDASEIDKYLDATAELSGLKILEALAASGLRSIRYFQQIPGVRSILVNDLDATAVESIKRNIKFNNVPSNRVIASRADASDILYSHRKESEQFDVIDLDPYGSAAMLLDGAVQAISNGGLICVTCTDMPVLSGKNPETCYSRYSSIPGKAQYVHENAIRIVLQAIERCAIKYQRHIVPVTSCSIDFYIRVFVRVFKSPYHVKKSVTKLSHVFQCVGCETFHLQPLGVATGN----TFHAARGPVVGTECGECGSKFRVTGPIWSDPLHDRETVLR----------VRAQVADSVASYPTKDRLYGLLTTISEEVIEAPLHYTISGLARTLHCTNPRLDQFQFAIRSQGYQVSQFHKAPDAIKTTAPPQTIWDIMRCWVMKHPINKKWENQNTPGNRILAKKSNLELDFNTRRPSKLQKEKVMRFPKNPQPYWGPKARAKGNRSNALDESVQEQK 521