EWM23818.1, cds6853 (CDS) Nannochloropsis gaditana

BLAST of EWM23818.1 vs. NCBI_GenBank
Match: gi|585105071|gb|EWM23818.1| (pentatricopeptide repeat-containing protein chloroplastic, partial [Nannochloropsis gaditana])

HSP 1 Score: 865.914 bits (2236), Expect = 0.000e+0
Identity = 424/424 (100.00%), Postives = 424/424 (100.00%), Query Frame = 0

Query:    1 PSLPPSLPSHLSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQLQWKKLTA 424
            PSLPPSLPSHLSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQLQWKKLTA
Sbjct:    1 PSLPPSLPSHLSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQLQWKKLTA 424          

BLAST of EWM23818.1 vs. NCBI_GenBank
Match: gi|1023176429|pdb|5I9F|A (Chain A, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U10 In Complex With Its Target Rna U10)

HSP 1 Score: 189.119 bits (479), Expect = 1.569e-51
Identity = 132/417 (31.65%), Postives = 214/417 (51.32%), Query Frame = 0

Query:    3 LPPSLPSHLSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            LPP      SR   LDV++Y  +L A L+ A  +  + A+E F  +L R+G AP  VT+NT++      GK   AL+    M ++ GI PDVV++ T+I    +    D A+++  +   E G++ D   Y   +     AG+  E L+LFE M          KG K               ++ YN +++ L    +  +AL++ +EM   G+ P+VV +NT++D + +AG+  EAL L ++M    +  D  TY   I  + K+  + +  +LFEEM   GI PD VTY TL++   +AG  ++  +L ++M  K G   DV  +  +I    +AG   EAL+L   M  + +KP VV+Y T +  L  +G+  EA +L E M  +G++P+ V      +    AG+LD+ ++L
Sbjct:    8 LPPG-----SR---LDVRAYTTVLHA-LSRAGRY--ERALELF-AELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM-VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMV--------EKGIKPD-------------VVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKL 388          

HSP 2 Score: 157.918 bits (398), Expect = 6.524e-40
Identity = 118/404 (29.21%), Postives = 199/404 (49.26%), Query Frame = 0

Query:   11 LSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLD 412
            + +GI  DV +YN L+   L +A      EA++ F  ++  +G  PD VT+NT++      GK   AL+    M ++ GI PDVV++ T+I    +    D A+++  +   E G++ D   Y   +     AG+  E L+LFE M          KG K               ++ YN +++ L    +  +AL++ +EM   G+ P+VV +NT++D + +AG+  EAL L ++M    +  D  TY   I  + K+  + +  +LFEEM   GI PD VTY TL++   +AG  ++  +L ++M  K G   DV  +  +I    +AG   EAL+L   M  + +KP  ++Y   + +   + R  EA   L  +    ++ ++  LE   E  +  GRL+
Sbjct:   78 VEKGIKPDVVTYNTLIDG-LCKAGKL--DEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM-VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMV--------EKGIKPD-------------VVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAYIEDAQ-FGRLE 452          

BLAST of EWM23818.1 vs. NCBI_GenBank
Match: gi|1023176433|pdb|5I9H|A (Chain A, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U8g2 In Complex With Its Target Rna U8g2)

HSP 1 Score: 187.963 bits (476), Expect = 3.778e-51
Identity = 132/417 (31.65%), Postives = 214/417 (51.32%), Query Frame = 0

Query:    3 LPPSLPSHLSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            LPP      SR   LDV++Y  +L A L+ A  +  + A+E F  +L R+G AP  VT+NT++      GK   AL+    M ++ GI PDVV++ T+I    +    D A+++  +   E G++ D   Y   +     AG+  E L+LFE M          KG K               ++ YN +++ L    +  +AL++ +EM   G+ P+VV + T++D + +AG+  EAL L ++M    +  D  TYT  I  + K+  + +  +LFEEM   GI PD VTY TL++   +AG  ++  +L ++M  K G   DV  +  +I    +AG   EAL+L   M  + +KP VV+Y T +  L  +G+  EA +L E M  +G++P+ V      +    AG+LD+ ++L
Sbjct:    8 LPPG-----SR---LDVRAYTTVLHA-LSRAGRY--ERALELF-AELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM-VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMV--------EKGIKPD-------------VVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKL 388          

HSP 2 Score: 157.147 bits (396), Expect = 1.590e-39
Identity = 118/404 (29.21%), Postives = 199/404 (49.26%), Query Frame = 0

Query:   11 LSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLD 412
            + +GI  DV +YN L+   L +A      EA++ F  ++  +G  PD VT+NT++      GK   AL+    M ++ GI PDVV++ T+I    +    D A+++  +   E G++ D   YT  +     AG+  E L+LFE M          KG K               ++ Y  +++ L    +  +AL++ +EM   G+ P+VV +NT++D + +AG+  EAL L ++M    +  D  TY   I  + K+  + +  +LFEEM   GI PD VTY TL++   +AG  ++  +L ++M  K G   DV  +  +I    +AG   EAL+L   M  + +KP  ++Y   + +   + R  EA   L  +    ++ ++  LE   E  +  GRL+
Sbjct:   78 VEKGIKPDVVTYNTLIDG-LCKAGKL--DEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM-VEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMV--------EKGIKPD-------------VVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAYIEDAQ-FGRLE 452          

BLAST of EWM23818.1 vs. NCBI_GenBank
Match: gi|929751352|ref|XP_014150659.1| (hypothetical protein SARC_10761 [Sphaeroforma arctica JP610] >gi|906943748|gb|KNC76757.1| hypothetical protein SARC_10761 [Sphaeroforma arctica JP610])

HSP 1 Score: 194.126 bits (492), Expect = 1.420e-50
Identity = 129/399 (32.33%), Postives = 208/399 (52.13%), Query Frame = 0

Query:   14 GIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLR-MMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAM-----QATQEKRKGGKERKGEGESEEDRLSVL---------IAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAA-GITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPN 394
            G+V +  +YN+    V+A   P  Q + V+  L ++       D   +N++L  + ++GK  LA++TL+ M K + GI P+V++++TVI ACG+   W+ AM +L + +  S +  +   Y AA+  C NAGR  E + L   M+       + T        E+    E+  + L+ +          AY+AV+ A  T  +W + LQV+QEM+  G  PN   +N+ + A   A + GEA++LL  M+ + VP +  TYT A     K+    +G  L  EM AA G   D   YT +++     G  E+ + L+  M R+RG P  V  + A++ AY  AG W++ ++ L TM+  NV    VSY T +AA A + +H +A ELL+ M R G+ P+
Sbjct:  350 GLVPNTTTYNS----VIATCVPDRQWKTVQGLLREMHDAKVPADLNIYNSVLSVYGAVGKWKLAVDTLKDMQKPEVGIRPNVITYSTVIAACGKAGQWEPAMALLKEMDINS-ITPNNYTYNAAVIACRNAGRWEETVRLLREMRKKGLRPNKYTYTSVISACEKTSRWEAALEVLADMGQAKLKPDAFAYSAVISACVTGGQWEKGLQVMQEMQSAGASPNTTTYNSCLSACERAEQWGEAMSLLNTMRAAGVPFNDSTYTLASVVCAKAGLWGEGMGLLREMAAAKGAKCDRKVYTNVIQACAAQGRWEEAQGLLADM-RERGVPPTVLTYNALLAAYKTAGKWKDTVQTLHTMRQENVAVDRVSYNTVLAACAQNAQHEKAAELLKDMGRAGILPD 742          

HSP 2 Score: 124.02 bits (310), Expect = 9.314e-27
Identity = 95/362 (26.24%), Postives = 163/362 (45.03%), Query Frame = 0

Query:   40 EAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLE 399
            EA    L D+ +    PD   ++ ++    + G+    L+ ++ M+  AG  P+  ++ + ++AC R   W  AM +L    + +GV  +   YT A  VCA AG   E + L   M A +  +  RK                     Y  V++A      W +A  +L +MR  G+ P V+ +N ++ A   AG+  + +  L  M+   V  D+ +Y   + A  ++A   +  EL ++M  AGI PD   Y+ ++  Y +AG  +    + + M  +RG  R    +T +I A  +A  W +A+ +L +M    V P   +Y  A+AA   + R   A +LL  M R  V P+ +   
Sbjct:  514 EAALEVLADMGQAKLKPDAFAYSAVISACVTGGQWEKGLQVMQEMQS-AGASPNTTTYNSCLSACERAEQWGEAMSLL-NTMRAAGVPFNDSTYTLASVVCAKAGLWGEGMGLLREMAAAKGAKCDRK--------------------VYTNVIQACAAQGRWEEAQGLLADMRERGVPPTVLTYNALLAAYKTAGKWKDTVQTLHTMRQENVAVDRVSYNTVLAACAQNAQHEKAAELLKDMGRAGILPDTFAYSNVLLAYEKAGQWKGAARVFRHMG-ERGVVRTTTTYTTMISACAKAKKWEDAIRVLDSMPRAGVTPDTSTYNAAIAACDQAARPNHAVKLLGDMVRACVSPDTITYN 852          

HSP 3 Score: 122.865 bits (307), Expect = 2.289e-26
Identity = 113/397 (28.46%), Postives = 172/397 (43.32%), Query Frame = 0

Query:   45 FLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRV-------LAQAEQ---ESGVEKDARMYTAAMRVCANAGRRREVL------------ELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
             LG++   G   D   +N  L   A  G    A E L  MK + G+ P V  +T+ + AC R  +W  A+R+       LA ++Q       E D +M T   +  A+A  R ++L            +L   +  M      + G    +             +   A+ +  ++           QE         V+ +N  + A A+  +   A+ LL+ M    + AD  TY+ AI A  K+    Q + L  EM A G+  +A TYT+     G+AG       ++  M  KRG   D +A+T+ I A  RAG W+ AL +L  M+A+NV P V +Y + ++A A + +  EA  LL  M   GVEPN  V   A  AC  AG+ D+ V L
Sbjct: 1048 LLGEVREDGLEIDGGMYNKGLYACAKKGASHTAQELLAEMKVK-GVDPTVQGYTSAVLACERAGEWQAAVRIVKEMGSTLASSDQLRAAVHTEVDTKMDTVGSQ--ADAVVREDILGSDLDTKERHTDDLSAHIDMMLDHTNNQAGVTHSQANPAHGGP-----VPKGAIPQQTQSQVREHAPASAAQERASDSHRQGVIAYNVAISACAKHRQGQWAIELLRDMTRVCLQADTVTYSTAIHACRKTGMHTQAQSLMREMQAKGVAVNAHTYTSNTVSKGEAGEWHSAVRVLDTM-EKRGVAPDTQAYTSAIAACARAGRWQTALTVLQRMRAKNVTPNVYTYSSVISACATASKWEEALALLRGMPNAGVEPNTFVYNAAISACGRAGQWDQAVGL 1435          

HSP 4 Score: 122.094 bits (305), Expect = 3.916e-26
Identity = 114/459 (24.84%), Postives = 192/459 (41.83%), Query Frame = 0

Query:   13 RGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSV--------------LIAYNAVLEALKT---DKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSY-----------------------------------GTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            RG+   V +YNALL A          ++ V+T L  + +   A DRV++NT+L   A   +   A E L+ M  +AGI+PD  +++ V+ A  +   W GA RV      E GV +    YT  +  CA A +  + + + + M     T +            ++     +V               I YN VL AL        W+ +L +L++M + G+ P+ + ++ V+ A +  G+    L +L+QM+   +  +   Y  +I A +K+   L    + ++M A GI  + VTY+ ++ L    G  E   EL ++M  +   P D R ++ +++A  +   W EAL+ + +M+     P +  Y                                      + A A  G    A ELL  MK +GV+P       A  AC  AG     V++
Sbjct:  667 RGVPPTVLTYNALLAAYKTAGK---WKDTVQT-LHTMRQENVAVDRVSYNTVLAACAQNAQHEKAAELLKDM-GRAGILPDTFAYSNVLLAYEKAGQWKGAARVFRHM-GERGVVRTTTTYTTMISACAKAKKWEDAIRVLDSMPRAGVTPDTSTYNAAIAACDQAARPNHAVKLLGDMVRACVSPDTITYNTVLSALTKGSGTNHWQVSLSLLRKMAQKGVQPDTITYSCVVAACSRGGQWQTILDILRQMRAQGIRGNTVIYNTSIAACVKAQQWLATFNILQDMRAEGIPTNTVTYSNVLSLCETLGKGEHALELYKEMRGEEVKP-DQRTYSILVQACAKGNRWHEALDFVSSMRHEGYPPGIGVYRWLFQGCVGAHQYDNALHLLGEVREDGLEIDGGMYNKGLYACAKKGASHTAQELLAEMKVKGVDPTVQGYTSAVLACERAGEWQAAVRI 1118          

HSP 5 Score: 108.997 bits (271), Expect = 9.641e-22
Identity = 101/384 (26.30%), Postives = 177/384 (46.09%), Query Frame = 0

Query:   54 FAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITAC---GRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEK-----------RKGGKERKGEGESEEDRLSVL---IAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELY--GQAGNKEQME-ELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            F     T  +++   A +  E + L  LR M    G   D   +T VI AC   GR W+ A  +LA   +E GV      Y A +     AG+ ++ ++    M+      ++           +    E+  E   +  R  +L    AY+ VL A +   +W+ A +V + M   G+V     + T++ A A+A +  +A+ +L  M  + V  D  TY AAI A  ++A      +L  +M  A ++PD +TY T++     G   N  Q+   L+++M++K G   D   ++ V+ A  R G W+  L++L  M+A+ ++   V Y T++AA   + + +  F +L+ M+ EG+  N V        C T G+ +  ++L
Sbjct:  600 FNDSTYTLASVVCAKAGLWGEGMGL--LREMAAAKGAKCDRKVYTNVIQACAAQGR-WEEAQGLLADM-RERGVPPTVLTYNALLAAYKTAGKWKDTVQTLHTMRQENVAVDRVSYNTVLAACAQNAQHEKAAELLKDMGRAGILPDTFAYSNVLLAYEKAGQWKGAARVFRHMGERGVVRTTTTYTTMISACAKAKKWEDAIRVLDSMPRAGVTPDTSTYNAAIAACDQAARPNHAVKLLGDMVRACVSPDTITYNTVLSALTKGSGTNHWQVSLSLLRKMAQK-GVQPDTITYSCVVAACSRGGQWQTILDILRQMRAQGIRGNTVIYNTSIAACVKAQQWLATFNILQDMRAEGIPTNTVTYSNVLSLCETLGKGEHALEL 978          

HSP 6 Score: 103.219 bits (256), Expect = 8.218e-20
Identity = 65/241 (26.97%), Postives = 118/241 (48.96%), Query Frame = 0

Query:  183 VLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTA------AIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
             L+ Y  +++A     +++  L++L EMR VGLVPN   +N+V+       +      LL++M  ++VPAD   Y +      A+     + D L+  +  E     GI P+ +TY+T++   G+AG  E    L+++M      P +   + A + A   AG W E + LL  M+ + ++P   +Y + ++A   + R   A E+L  M +  ++P+         AC T G+ +K +Q+
Sbjct:  319 TLVTYTTMIDAYAAGGQYKFGLELLAEMRTVGLVPNTTTYNSVIATCVPDRQWKTVQGLLREMHDAKVPADLNIYNSVLSVYGAVGKWKLAVDTLKDMQKPE----VGIRPNVITYSTVIAACGKAGQWEPAMALLKEMDINSITPNNY-TYNAAVIACRNAGRWEETVRLLREMRKKGLRPNKYTYTSVISACEKTSRWEAALEVLADMGQAKLKPDAFAYSAVISACVTGGQWEKGLQV 554          

HSP 7 Score: 100.523 bits (249), Expect = 5.725e-19
Identity = 82/346 (23.70%), Postives = 149/346 (43.06%), Query Frame = 0

Query:   46 LGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR-----DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAM 386
            L  + R G  PD  T+N  +       +   A++ L  M  +A + PD +++ TV++A  +      W  ++ +L +  Q+ GV+ D   Y+  +  C+  G+ + +L++  +M+A     +  +G                  + YN  + A    ++W     +LQ+MR  G+  N V ++ V+      G+   AL L K+M+   V  D+RTY+  ++A  K     +  +    M   G  P    Y  L +    A   +    L+ ++ R+ G   D   +   + A  + G    A ELL  MK + V PTV  Y +A+ A   +G    A  +++ M
Sbjct:  801 LDSMPRAGVTPDTSTYNAAIAACDQAARPNHAVKLLGDMV-RACVSPDTITYNTVLSALTKGSGTNHWQVSLSLLRKMAQK-GVQPDTITYSCVVAACSRGGQWQTILDILRQMRA-----QGIRGNT----------------VIYNTSIAACVKAQQWLATFNILQDMRAEGIPTNTVTYSNVLSLCETLGKGEHALELYKEMRGEEVKPDQRTYSILVQACAKGNRWHEALDFVSSMRHEGYPPGIGVYRWLFQGCVGAHQYDNALHLLGEV-REDGLEIDGGMYNKGLYACAKKGASHTAQELLAEMKVKGVDPTVQGYTSAVLACERAGEWQAAVRIVKEM 1122          

HSP 8 Score: 90.8929 bits (224), Expect = 7.336e-16
Identity = 59/232 (25.43%), Postives = 106/232 (45.69%), Query Frame = 0

Query:  187 YNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKR-GFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            YN V+ +L   ++ + A  V   M    +   +V + T++DA A  G+    L LL +M+   +  +  TY + I   +        + L  EM  A +  D   Y +++ +YG  G  +   + ++ M +   G   +V  ++ VI A G+AG W  A+ LL  M   ++ P   +Y  A+ A   +GR  E   LL  M+++G+ PN+        AC    R +  +++
Sbjct:  288 YNRVIMSLVHARDTKGARAVFDTMLTHKIARTLVTYTTMIDAYAAGGQYKFGLELLAEMRTVGLVPNTTTYNSVIATCVPDRQWKTVQGLLREMHDAKVPADLNIYNSVLSVYGAVGKWKLAVDTLKDMQKPEVGIRPNVITYSTVIAACGKAGQWEPAMALLKEMDINSITPNNYTYNAAVIACRNAGRWEETVRLLREMRKKGLRPNKYTYTSVISACEKTSRWEAALEV 519          

HSP 9 Score: 87.4261 bits (215), Expect = 1.014e-14
Identity = 80/338 (23.67%), Postives = 139/338 (41.12%), Query Frame = 0

Query:   14 GIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILK---HHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKA-------------MQATQEKRKG------GKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRA 327
            G+  D  +YNA + A    A P     AV+  LGD+ R   +PD +T+NT+L      +      ++L  LR M  Q G+ PD ++++ V+ AC R   W   + +L Q   + G+  +  +Y  ++  C  A +      + + M+A             +   +   KG       KE +GE    + R      Y+ +++A      W +AL  +  MR  G  P +  +  +      A +   AL LL +++   +  D   Y   + A  K       +EL  EM   G+ P    YT+ +    +AG  +    +V++M         +RA
Sbjct:  808 GVTPDTSTYNAAIAACDQAARP---NHAVK-LLGDMVRACVSPDTITYNTVLSALTKGSGTNHWQVSLSLLRKMA-QKGVQPDTITYSCVVAACSRGGQWQTILDILRQMRAQ-GIRGNTVIYNTSIAACVKAQQWLATFNILQDMRAEGIPTNTVTYSNVLSLCETLGKGEHALELYKEMRGEEVKPDQR-----TYSILVQACAKGNRWHEALDFVSSMRHEGYPPGIGVYRWLFQGCVGAHQYDNALHLLGEVREDGLEIDGGMYNKGLYACAKKGASHTAQELLAEMKVKGVDPTVQGYTSAVLACERAGEWQAAVRIVKEMGSTLASSDQLRA 1134          

HSP 10 Score: 87.4261 bits (215), Expect = 1.014e-14
Identity = 88/330 (26.67%), Postives = 136/330 (41.21%), Query Frame = 0

Query:   48 DLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGRDWDGAMRVLAQA----EQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLM---ELYGQAGNKEQ-MEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAA 369
            D  R+G     V  +   KH     +   A+E LR M  +  +  D V+++T I AC +     M   AQ+     Q  GV  +A  YT+       AG     + + + M              E++G     +       AY + + A      W+ AL VLQ MR   + PNV  +++V+ A A A +  EALALL+ M  + V  +   Y AAI A  ++    Q   L  EM + G+  D +TY   +    + GQ     Q +EE+  +   K     D   +T V +A      W     +   M   NV P+  +Y  A++A
Sbjct: 1232 DSHRQGVIAYNVAISACAKHR----QGQWAIELLRDMT-RVCLQADTVTYSTAIHACRKT---GMHTQAQSLMREMQAKGVAVNAHTYTSNTVSKGEAGEWHSAVRVLDTM--------------EKRGVAPDTQ-------AYTSAIAACARAGRWQTALTVLQRMRAKNVTPNVYTYSSVISACATASKWEEALALLRGMPNAGVEPNTFVYNAAISACGRAGQWDQAVGLVREMESKGVARDTITYNNAILGAAIGGQCAVALQLLEEMSDKSKGKVQTQPDTITYTCVFKACESGLEWENIPRIWKRMLGDNVTPSRRTYDAAVSA 1532          

HSP 11 Score: 87.0409 bits (214), Expect = 1.328e-14
Identity = 55/202 (27.23%), Postives = 96/202 (47.52%), Query Frame = 0

Query:  218 NVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNV--KPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            +  ++N V+ ++  A +   A A+   M   ++     TYT  I A         G EL  EM   G+ P+  TY +++         + ++ L+++M   +  P D+  + +V+  YG  G W+ A++ L  M+   V  +P V++Y T +AA   +G+   A  LL+ M    + PN      A  ACR AGR ++ V+L
Sbjct:  284 SATHYNRVIMSLVHARDTKGARAVFDTMLTHKIARTLVTYTTMIDAYAAGGQYKFGLELLAEMRTVGLVPNTTTYNSVIATCVPDRQWKTVQGLLREMHDAK-VPADLNIYNSVLSVYGAVGKWKLAVDTLKDMQKPEVGIRPNVITYSTVIAACGKAGQWEPAMALLKEMDINSITPNNYTYNAAVIACRNAGRWEETVRL 484          

HSP 12 Score: 70.0922 bits (170), Expect = 4.219e-9
Identity = 59/228 (25.88%), Postives = 99/228 (43.42%), Query Frame = 0

Query:   46 LGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACG--RDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMR-----RVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIK 266
            L  + +RG APD   + + +   A  G+   AL  L+ M+ +  + P+V ++++VI+AC     W+ A+ +L +    +GVE +  +Y AA+  C  AG+  + + L   M              E KG            I YN  +       +   ALQ+L+EM      +V   P+ + +  V  A     E      + K+M    V   +RTY AA+ A +K
Sbjct: 1331 LDTMEKRGVAPDTQAYTSAIAACARAGRWQTALTVLQRMRAK-NVTPNVYTYSSVISACATASKWEEALALL-RGMPNAGVEPNTFVYNAAISACGRAGQWDQAVGLVREM--------------ESKGVARD-------TITYNNAILGAAIGGQCAVALQLLEEMSDKSKGKVQTQPDTITYTCVFKACESGLEWENIPRIWKRMLGDNVTPSRRTYDAAVSASVK 1535          

BLAST of EWM23818.1 vs. NCBI_GenBank
Match: gi|299471045|emb|CBN78905.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 189.504 bits (480), Expect = 1.131e-49
Identity = 131/456 (28.73%), Postives = 215/456 (47.15%), Query Frame = 0

Query:   12 SRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQ-----------------------AEQESGVE-----------KDARMYTAAMRVCANAGRRREVLELFERMKA--MQATQEKRKGGKERKGEGESEEDRLSV------------LIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            S G+  D  +YN+++     E     Q +   + L ++S +G  P+ +++N  ++     G+   A+E LR M+ Q G+ PDV+S+   I ACG    W+ ++ +L +                        + E  VE            DA  + +A+  C  +GR +E LEL + M A  ++          E  G+G   E  L +            +I+ N  + A      W++ L++L++M   GL PNV+ +N+ +    + G+  +AL LL +MK   +  D  TY +AI A  K     +   L  EM   G+TPD ++YT  ++ YG+ G  E+  EL++QM  K G   +V  + +VI+A GR G W +AL+LL  +K   V P ++SY  A++A    GR  EA +LL  M  EG+ P+ +    A  AC   G  +K + L
Sbjct:  243 SVGLTPDAITYNSVITGCGKEG----QWKEALSVLTEMSAKGLTPETISYNMAIRACGRSGRWKEAVEVLRQMESQ-GVTPDVISYDAAIKACGGGGQWETSVDLLDEMAGRGVAPNTIHFNSAIVACGKGGQWEKAVELLREVTALGLTPDATSFNSAIAACTKSGRWKEALELLKEMPAKRLKPDAISYNSAIEACGKGGQWEMALELRRQMPTKGLTPNVISSNIAIRACGERGRWQEGLELLRQMPAQGLTPNVITYNSAIKTCGKGGQWEKALDLLAKMKELAMTPDSITYNSAIAACSKRGRWKEAVALLREMPGQGLTPDVISYTAAIDAYGKNGQWERAVELLRQMPTK-GLTPNVITYNSVIKACGRGGEWEKALDLLKELKETAVAPDLMSYNLAISACGKRGRWEEALDLLREMPAEGLTPDVISYTSAIRACNAEGEWEKALGL 692          

HSP 2 Score: 177.563 bits (449), Expect = 2.384e-45
Identity = 123/422 (29.15%), Postives = 205/422 (48.58%), Query Frame = 0

Query:   12 SRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGRD--WDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQE--------KRKGGKERKGEG------ESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            S+G+  DV SY+A + A         Q E     L +++ RG AP+ + FN+ +      G+   A+E LR +    G+ PD  SF + I AC +   W  A+ +L +   +  ++ DA  Y +A+  C   G+    LEL  +M     T          +  G + R  EG         +     +I YN+ ++      +W +AL +L +M+ + + P+ + +N+ + A ++ G   EA+ALL++M    +  D  +YTAAI A  K+    +  EL  +M   G+TP+ +TY ++++  G+ G  E+  +L++++      P D+ ++   I A G+ G W EAL+LL  M A  + P V+SY +A+ A  A G   +A  LL  M   GV P       A EAC   GR ++ V L
Sbjct:  313 SQGVTPDVISYDAAIKACGGGG----QWETSVDLLDEMAGRGVAPNTIHFNSAIVACGKGGQWEKAVELLREVT-ALGLTPDATSFNSAIAACTKSGRWKEALELLKEMPAKR-LKPDAISYNSAIEACGKGGQWEMALELRRQMPTKGLTPNVISSNIAIRACGERGRWQEGLELLRQMPAQGLTPNVITYNSAIKTCGKGGQWEKALDLLAKMKELAMTPDSITYNSAIAACSKRGRWKEAVALLREMPGQGLTPDVISYTAAIDAYGKNGQWERAVELLRQMPTKGLTPNVITYNSVIKACGRGGEWEKALDLLKELKETAVAP-DLMSYNLAISACGKRGRWEEALDLLREMPAEGLTPDVISYTSAIRACNAEGEWEKALGLLNLMGAHGVSPTATSYSLAIEACGKGGRREEAVCL 727          

HSP 3 Score: 158.303 bits (399), Expect = 1.126e-38
Identity = 107/392 (27.30%), Postives = 189/392 (48.21%), Query Frame = 0

Query:   14 GIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGRD--WDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVE----PNQVVLE 399
            G+  D  S+N+   A+ A       +EA+E  L ++  +   PD +++N+ ++     G+  +ALE  R M  + G+ P+V+S    I ACG    W   + +L Q   + G+  +   Y +A++ C   G+  + L+L  +MK +  T +                      I YN+ + A      W++A+ +L+EM   GL P+V+++   +DA  + G+   A+ LL+QM    +  +  TY + IKA  +  +  +  +L +E+    + PD ++Y   +   G+ G  E+  +L+++M  + G   DV ++T+ IRA    G W +AL LL  M A  V PT  SY  A+ A    GR  EA  L+  M + G+      N +++E
Sbjct:  385 GLTPDATSFNS---AIAACTKSGRWKEALE-LLKEMPAKRLKPDAISYNSAIEACGKGGQWEMALELRRQMPTK-GLTPNVISSNIAIRACGERGRWQEGLELLRQMPAQ-GLTPNVITYNSAIKTCGKGGQWEKALDLLAKMKELAMTPDS---------------------ITYNSAIAACSKRGRWKEAVALLREMPGQGLTPDVISYTAAIDAYGKNGQWERAVELLRQMPTKGLTPNVITYNSVIKACGRGGEWEKALDLLKELKETAVAPDLMSYNLAISACGKRGRWEEALDLLREMPAE-GLTPDVISYTSAIRACNAEGEWEKALGLLNLMGAHGVSPTATSYSLAIEACGKGGRREEAVCLVREMAQRGLSHRNISNSLIVE 748          

HSP 4 Score: 157.918 bits (398), Expect = 1.505e-38
Identity = 100/356 (28.09%), Postives = 177/356 (49.72%), Query Frame = 0

Query:   65 LKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGRD--WDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQLQ 418
            L+  A+ GK   AL  L  MK+  G V    S+TT IT CGR   W+ A+ +L +  ++ GV  +   Y +A+  C +  +    + L   M   +   ++                     I+YN+ ++A     +W + + +L+EM  VGL P+ + +N+V+    + G+  EAL++L +M    +  +  +Y  AI+A  +S    +  E+  +M + G+TPD ++Y   ++  G  G  E   +L+ +M+  RG   +   F + I A G+ G W +A+ELL  + A  + P   S+ +A+AA   SGR  EA ELL+ M  + ++P+ +    A EAC   G+ +  ++L+
Sbjct:  117 LREAATTGKWREALGILDEMKE--GGVVCAHSYTTAITTCGRQGQWEKALELLREIPEQEGVSPNVFCYNSAIEACGSGDQWEIAVSLLREMADREVVPDE---------------------ISYNSAIKACGRGGQWERVIGLLREMPSVGLTPDAITYNSVITGCGKEGQWKEALSVLTEMSAKGLTPETISYNMAIRACGRSGRWKEAVEVLRQMESQGVTPDVISYDAAIKACGGGGQWETSVDLLDEMA-GRGVAPNTIHFNSAIVACGKGGQWEKAVELLREVTALGLTPDATSFNSAIAACTKSGRWKEALELLKEMPAKRLKPDAISYNSAIEACGKGGQWEMALELR 448          

HSP 5 Score: 95.9005 bits (237), Expect = 1.298e-17
Identity = 71/291 (24.40%), Postives = 129/291 (44.33%), Query Frame = 0

Query:   12 SRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGRD--WDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYG 300
            ++G+  +V +YN+ +           Q E     L  +      PD +T+N+ +   +  G+   A+  LR M  Q G+ PDV+S+T  I A G++  W+ A+ +L Q   + G+  +   Y + ++ C   G   + L+L + +K      +                     L++YN  + A      W +AL +L+EM   GL P+V+++ + + A    GE  +AL LL  M    V     +Y+ AI+A  K     +   L  EM   G++   ++ + ++E  G
Sbjct:  488 AQGLTPNVITYNSAIKTCGKGG----QWEKALDLLAKMKELAMTPDSITYNSAIAACSKRGRWKEAVALLREMPGQ-GLTPDVISYTAAIDAYGKNGQWERAVELLRQMPTK-GLTPNVITYNSVIKACGRGGEWEKALDLLKELKETAVAPD---------------------LMSYNLAISACGKRGRWEEALDLLREMPAEGLTPDVISYTSAIRACNAEGEWEKALGLLNLMGAHGVSPTATSYSLAIEACGKGGRREEAVCLVREMAQRGLSHRNISNSLIVEALG 751          

HSP 6 Score: 69.707 bits (169), Expect = 4.237e-9
Identity = 59/232 (25.43%), Postives = 112/232 (48.28%), Query Frame = 0

Query:   18 DVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKA-------MQATQEKRKGGKERKGEGESEEDRLSVL---------IAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAE 231
            D  +YN+   A+ A +     +EAV   L ++  +G  PD +++   +  +   G+   A+E LR M  + G+ P+V+++ +VI ACGR  +W+ A+ +L +  +E+ V  D   Y  A+  C   GR  E L+L   M A       +  T   R    E  GE E     L+++          +Y+  +EA        +A+ +++EM + GL    ++++ +++A+ +
Sbjct:  529 DSITYNS---AIAACSKRGRWKEAV-ALLREMPGQGLTPDVISYTAAIDAYGKNGQWERAVELLRQMPTK-GLTPNVITYNSVIKACGRGGEWEKALDLLKEL-KETAVAPDLMSYNLAISACGKRGRWEEALDLLREMPAEGLTPDVISYTSAIRACNAE--GEWEKALGLLNLMGAHGVSPTATSYSLAIEACGKGGRREEAVCLVREMAQRGLSHRNISNSLIVEALGD 752          

BLAST of EWM23818.1 vs. NCBI_GenBank
Match: gi|1023176431|pdb|5I9G|C (Chain C, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U8c2 In Complex With Its Target Rna U8c2)

HSP 1 Score: 183.726 bits (465), Expect = 1.938e-49
Identity = 129/417 (30.94%), Postives = 211/417 (50.60%), Query Frame = 0

Query:    3 LPPSLPSHLSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            LPP        G  LDV++Y  +L A L+ A  +  + A+E F  +L R+G AP  VT+NT++      GK   AL+    M ++ GI PDVV++ T+I    +    D A+++  +   E G++ D   Y   +     AG+  E L+LFE M          KG K               ++ YN +++ L    +  +AL++ +EM   G+ P+VV +NT++D + +AG+  EAL L ++M    +     TY   I  + K+  + +  +LFEEM   GI P  VTY TL++   +AG  ++  +L ++M  K G   DV  +  +I    +AG   EAL+L   M  + +KP VV+Y T +  L  +G+  EA +L E M  +G++P+ V      +    AG+LD+ ++L
Sbjct:    8 LPP--------GSRLDVRAYTTVLHA-LSRAGRY--ERALELF-AELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM-VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMV--------EKGIKPD-------------VVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKL 388          

HSP 2 Score: 157.532 bits (397), Expect = 8.514e-40
Identity = 118/404 (29.21%), Postives = 199/404 (49.26%), Query Frame = 0

Query:   11 LSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLD 412
            + +GI  DV +YN L+   L +A      EA++ F  ++  +G  PD VT+NT++      GK   AL+    M ++ GI PDVV++ T+I    +    D A+++  +   E G++ D   Y   +     AG+  E L+LFE M          KG K               ++ YN +++ L    +  +AL++ +EM   G+ P+VV +NT++D + +AG+  EAL L ++M    +  D  TY   I  + K+  + +  +LFEEM   GI PD VTY TL++   +AG  ++  +L ++M  K G   DV  +  +I    +AG   EAL+L   M  + +KP  ++Y   + +   + R  EA   L  +    ++ ++  LE   E  +  GRL+
Sbjct:   78 VEKGIKPDVVTYNTLIDG-LCKAGKL--DEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM-VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMV--------EKGIKPS-------------VVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAYIEDAQ-FGRLE 452          

BLAST of EWM23818.1 vs. NCBI_GenBank
Match: gi|1023176427|pdb|5I9D|A (Chain A, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U8a2 In Complex With Its Target Rna U8a2)

HSP 1 Score: 183.341 bits (464), Expect = 2.338e-49
Identity = 128/417 (30.70%), Postives = 214/417 (51.32%), Query Frame = 0

Query:    3 LPPSLPSHLSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            LPP        G  LDV++Y  +L A L+ A  +  + A+E F  +L R+G AP  VT+NT++      GK   AL+    M ++ GI PDVV++ T+I    +    D A+++  +   E G++ D   Y   +     AG+  E L+LFE M          KG K               ++ YN +++ L    +  +AL++ +EM   G+ P+VV ++T++D + +AG+  EAL L ++M    +  +  TY+  I  + K+  + +  +LFEEM   GI P+ VTY TL++   +AG  ++  +L ++M  K G   DV  +  +I    +AG   EAL+L   M  + +KP VV+Y T +  L  +G+  EA +L E M  +G++P+ V      +    AG+LD+ ++L
Sbjct:    8 LPP--------GSRLDVRAYTTVLHA-LSRAGRY--ERALELF-AELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM-VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMV--------EKGIKPD-------------VVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKL 388          

HSP 2 Score: 158.303 bits (399), Expect = 4.420e-40
Identity = 118/404 (29.21%), Postives = 200/404 (49.50%), Query Frame = 0

Query:   11 LSRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLD 412
            + +GI  DV +YN L+   L +A      EA++ F  ++  +G  PD VT+NT++      GK   AL+    M ++ GI PDVV++ T+I    +    D A+++  +   E G++ D   Y+  +     AG+  E L+LFE M          KG K               ++ Y+ +++ L    +  +AL++ +EM   G+ PNVV +NT++D + +AG+  EAL L ++M    +  D  TY   I  + K+  + +  +LFEEM   GI PD VTY TL++   +AG  ++  +L ++M  K G   DV  +  +I    +AG   EAL+L   M  + +KP  ++Y   + +   + R  EA   L  +    ++ ++  LE   E  +  GRL+
Sbjct:   78 VEKGIKPDVVTYNTLIDG-LCKAGKL--DEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM-VEKGIKPDVVTYSTLIDGLCKAGKLDEALKLFEEMV--------EKGIKPN-------------VVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAYIEDAQ-FGRLE 452          

BLAST of EWM23818.1 vs. NCBI_GenBank
Match: gi|551540996|ref|XP_005758827.1| (hypothetical protein EMIHUDRAFT_53066, partial [Emiliania huxleyi CCMP1516] >gi|485606664|gb|EOD06398.1| hypothetical protein EMIHUDRAFT_53066, partial [Emiliania huxleyi CCMP1516])

HSP 1 Score: 174.096 bits (440), Expect = 3.754e-46
Identity = 126/410 (30.73%), Postives = 199/410 (48.54%), Query Frame = 0

Query:   14 GIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITAC---GRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQLQWK 420
            G+  +V SYNA + +V  +A    Q +   + L ++   G  P+ +++N  +      G+   AL  LR M D  G+ PDV+S++  I+AC   GR  D A+ +L +    +GV+ +   Y AA+  C  AGR  E + L   M A                           +++Y+A + A +      +AL +L+EM   GL PNV+++N  + A  +AG   EAL+LL++M  + V  D  +Y AAI A  K+    +   L  EM +AG+ PD ++Y+  +    +A   ++   L+++M   R  P DV ++ A I +  +AG   EAL LL  M    + P   SY  A++A   +GR  EA  LL  M   G+ PN +    A  AC  AGR D+ + L  K
Sbjct:   40 GVQPNVISYNAAI-SVCEKAS---QWKKALSLLREMPGAGVQPNVISYNAAISACEKAGRCDEALSLLREMPD-VGVQPDVISYSAAISACEKAGR-CDEALSLLREMPG-AGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPN---------------------VVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSAGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQP-DVISYNAAISSCEKAGRCNEALSLLREMPDAGLSPNEYSYNAAISACEKAGRCNEALSLLREMPGAGLTPNVISYNAAISACEKAGRCDEALSLLSK 420          

HSP 2 Score: 174.096 bits (440), Expect = 4.343e-46
Identity = 120/379 (31.66%), Postives = 189/379 (49.87%), Query Frame = 0

Query:   44 TFLGDLSRRGFAPDRVTFNTILK--HHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITAC---GRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            + L ++   G  P+ +++N  +     AS  K+AL+L  LR M   AG+ P+V+S+   I+AC   GR  D A+ +L +   + GV+ D   Y+AA+  C  AGR  E L L                 +E  G G    +      +YNA + A +    W +A+ +L+EM   GL PNVV+++  + A  +AG   EAL+LL++M  + +  +  +Y AAI A  K+    +   L  EM  AG+ PD ++Y   +    +AG   +   L+++M    G   DV +++A I A  +A    EAL LL  M    V+P V+SY  A+++   +GR  EA  LL  M   G+ PN+     A  AC  AGR ++ + L
Sbjct:   31 SLLREMPNAGVQPNVISYNAAISVCEKASQWKKALSL--LREMPG-AGVQPNVISYNAAISACEKAGR-CDEALSLLREM-PDVGVQPDVISYSAAISACEKAGRCDEALSLL----------------REMPGAGVQPNE-----YSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSA-GVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGLSPNEYSYNAAISACEKAGRCNEALSL 382          

HSP 3 Score: 161.384 bits (407), Expect = 1.980e-41
Identity = 106/333 (31.83%), Postives = 170/333 (51.05%), Query Frame = 0

Query:   87 QAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            QAG  P+V S++ +I  C R  DW  A+ +L +    +GV+ +   Y AA+ VC  A + ++ L L                 +E  G G         +I+YNA + A +      +AL +L+EM  VG+ P+V++++  + A  +AG   EAL+LL++M  + V  ++ +Y AAI A  K+    +   L  EM AAG+ P+ V+Y+  +    +AG  ++   L+++M    G   +V ++ A I A  +AG   EAL LL  M    V+P V+SY  A++A   +GR  EA  LL  M   GV+P+ +    A  AC  A R D+ + L
Sbjct:    3 QAGCEPNVFSYSILINLCARSKDWKTAVSLLREMPN-AGVQPNVISYNAAISVCEKASQWKKALSLL----------------REMPGAGVQPN-----VISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMP-DAGLSPNVISYNAAISACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSAGVQPDVISYSAAISACEKASRCDEALSL 312          

HSP 4 Score: 133.65 bits (335), Expect = 3.402e-31
Identity = 107/386 (27.72%), Postives = 174/386 (45.08%), Query Frame = 0

Query:   14 GIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITAC---GRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQV 396
            G+  DV SY+A + A         + +   + L ++   G  P+  ++N  +      G+   A+  LR M   AG+ P+VVS++  I+AC   GR  D A+ +L +   ++G+  +   Y AA+  C  AGR  E L L                 +E  G G   +     +I+YNA + A +      +AL +L+EM   G+ P+V++++  + A  +A    EAL+LL++M  +RV  D  +Y AAI +  K+    +   L  EM  AG++P+  +Y                                     A I A  +AG   EAL LL  M    + P V+SY  A++A   +GR  EA  LL  M+ +GV+P+ +
Sbjct:  110 GVQPDVISYSAAISACEKAG----RCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMP-AAGLRPNVVSYSAAISACEKAGR-CDEALSLLREM-PDAGLSPNVISYNAAISACEKAGRCDEALSLL----------------REMPGAGVQPD-----VISYNAAISACEKAGRGNEALSLLREMPSAGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGLSPNEYSY------------------------------------NAAISACEKAGRCNEALSLLREMPGAGLTPNVISYNAAISACEKAGRCDEALSLLSKMQEDGVQPDVI 431          

HSP 5 Score: 107.457 bits (267), Expect = 7.508e-22
Identity = 67/208 (32.21%), Postives = 108/208 (51.92%), Query Frame = 0

Query:  210 MRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            MR+ G  PNV +++ +++  A + +   A++LL++M  + V  +  +Y AAI    K++   +   L  EM  AG+ P+ ++Y   +    +AG  ++   L+++M    G   DV +++A I A  +AG   EAL LL  M    V+P   SY  A++A   +GR  EA  LL  M   G+ PN V    A  AC  AGR D+ + L
Sbjct:    1 MRQAGCEPNVFSYSILINLCARSKDWKTAVSLLREMPNAGVQPNVISYNAAISVCEKASQWKKALSLLREMPGAGVQPNVISYNAAISACEKAGRCDEALSLLREMP-DVGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSL 207          

BLAST of EWM23818.1 vs. NCBI_GenBank
Match: gi|299471535|emb|CBN80021.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 177.563 bits (449), Expect = 4.593e-46
Identity = 110/408 (26.96%), Postives = 204/408 (50.00%), Query Frame = 0

Query:   12 SRGIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGRD--WDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            ++GI  D +SY A + A         + +     L ++  R   P+ + +N+ +   A  G+  +A+  ++ M    G+ PDV+S+++ I+ACGR   W+ A+ +  +  + SGV  D   Y +A+  CAN GR +E + L  ++  +  T                       +I+Y++V+ A   + +W+ AL +L+EM+ + L PN++ +N  +DA A+ G   EA+ LL++M    +P D  +Y++ I A  K     +  ++  EM   G++P+A++Y + ++   + G  ++ + L+++M    G  + V  + + I A  +   W+EAL LL  M    +  TV SY +A+ A       ++A ELL  M   G+ PN+V    A +AC    + ++ V L
Sbjct:   96 TKGIAPDARSYGAAINACARGG----RWKIALDLLREMLARDVTPNVIIYNSAINSCAKAGQWEIAVSLVKEMA-TVGLAPDVISYSSAISACGRGGRWEEALELF-EDMRTSGVAPDVITYGSAIAACANGGRWKEAVSLLRKIPTVGLTPN---------------------VISYSSVITACAKEGQWKIALDLLREMKAMRLAPNIITYNAAIDACAKGGRWKEAIDLLREMPTVGLPPDVVSYSSVIDACSKGDRWKEAIDILREMPTVGLSPNAISYNSAIDACAKGGQWKEAKGLLREMPTA-GVTQRVIGYNSAIAACAKGEQWKEALALLREMPTVGLHTTVFSYSSAIDACGKGNLWIKAKELLREMATVGLAPNEVCYNSAIDACGRGDQWEEAVDL 475          

HSP 2 Score: 170.629 bits (431), Expect = 1.609e-43
Identity = 115/406 (28.33%), Postives = 202/406 (49.75%), Query Frame = 0

Query:   14 GIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITACGRD--WDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            G+  DV SY++   A+ A       +EA+E F  D+   G APD +T+ + +   A+ G+   A+  LR +    G+ P+V+S+++VITAC ++  W  A+ +L + +    +  +   Y AA+  CA  GR +E ++L   M  +    +                     +++Y++V++A      W++A+ +L+EM  VGL PN +++N+ +DA A+ G+  EA  LL++M  + V      Y +AI A  K     +   L  EM   G+     +Y++ ++  G+     + +EL+++M+     P +V  + + I A GR   W EA++LL  M    + P V SY +A+AA A   +  EA  +L+ M   G+ P+ +    A +AC   GR    V L
Sbjct:  168 GLAPDVISYSS---AISACGRGGRWEEALELF-EDMRTSGVAPDVITYGSAIAACANGGRWKEAVSLLRKIP-TVGLTPNVISYSSVITACAKEGQWKIALDLLREMKAMR-LAPNIITYNAAIDACAKGGRWKEAIDLLREMPTVGLPPD---------------------VVSYSSVIDACSKGDRWKEAIDILREMPTVGLSPNAISYNSAIDACAKGGQWKEAKGLLREMPTAGVTQRVIGYNSAIAACAKGEQWKEALALLREMPTVGLHTTVFSYSSAIDACGKGNLWIKAKELLREMATVGLAPNEV-CYNSAIDACGRGDQWEEAVDLLREMPTVGLSPDVFSYSSAIAACAKGDQWKEALAVLKEMSAAGLAPDLICYNSAIDACSKGGRWKMAVAL 545          

HSP 3 Score: 145.976 bits (367), Expect = 9.690e-35
Identity = 96/343 (27.99%), Postives = 165/343 (48.10%), Query Frame = 0

Query:   77 ALETLRMMKDQAGIVPDVVSFTTVITACGR--DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            AL  +  MK   G + D  S+   ITACGR   W+ A+R+L +   E G   D   Y+A +  CA  G+ +  +     M       + R                     +Y A + A      W+ AL +L+EM    + PNV+ +N+ +++ A+AG+   A++L+K+M    +  D  +Y++AI A  +     +  ELFE+M  +G+ PD +TY + +      G  ++   L++++    G   +V ++++VI A  + G W+ AL+LL  MKA  + P +++Y  A+ A A  GR  EA +LL  M   G+ P+ V      +AC    R  + + +
Sbjct:   16 ALRAVDGMKKLPGWLIDQKSYCMAITACGRGGQWEQAVRLLREMPTE-GAAPDLSAYSAVIDACAKGGQWKMAVFFLMEMPTKGIAPDAR---------------------SYGAAINACARGGRWKIALDLLREMLARDVTPNVIIYNSAINSCAKAGQWEIAVSLVKEMATVGLAPDVISYSSAISACGRGGRWEEALELFEDMRTSGVAPDVITYGSAIAACANGGRWKEAVSLLRKIP-TVGLTPNVISYSSVITACAKEGQWKIALDLLREMKAMRLAPNIITYNAAIDACAKGGRWKEAIDLLREMPTVGLPPDVVSYSSVIDACSKGDRWKEAIDI 335          

HSP 4 Score: 128.257 bits (321), Expect = 1.607e-28
Identity = 85/311 (27.33%), Postives = 140/311 (45.02%), Query Frame = 0

Query:  107 DWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            DW  A+R +   ++  G   D + Y  A+  C   G+  + + L   M                  EG + +     L AY+AV++A     +W+ A+  L EM   G+ P+  ++   ++A A  G    AL LL++M    V  +   Y +AI +  K+        L +EM   G+ PD ++Y++ +   G+ G  E+  EL + M R  G   DV  + + I A    G W+EA+ LL  +    + P V+SY + + A A  G+   A +LL  MK   + PN +    A +AC   GR  + + L
Sbjct:   12 DWRKALRAVDGMKKLPGWLIDQKSYCMAITACGRGGQWEQAVRLLREMPT----------------EGAAPD-----LSAYSAVIDACAKGGQWKMAVFFLMEMPTKGIAPDARSYGAAINACARGGRWKIALDLLREMLARDVTPNVIIYNSAINSCAKAGQWEIAVSLVKEMATVGLAPDVISYSSAISACGRGGRWEEALELFEDM-RTSGVAPDVITYGSAIAACANGGRWKEAVSLLRKIPTVGLTPNVISYSSVITACAKEGQWKIALDLLREMKAMRLAPNIITYNAAIDACAKGGRWKEAIDL 300          

HSP 5 Score: 114.775 bits (286), Expect = 6.928e-24
Identity = 100/409 (24.45%), Postives = 167/409 (40.83%), Query Frame = 0

Query:   14 GIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITAC--GRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQE--KRKGGKERKGEGESEEDRLSVL-----------------------------------------------IAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALA 371
            G+  +V SY++++ A   E     Q +     L ++     AP+ +T+N  +   A  G+   A++ LR M    G+ PDVVS+++VI AC  G  W  A+ +L +     G+  +A  Y +A+  CA  G+ +E   L   M     TQ             +GE  ++ L++L                                               + YN+ ++A     +W +A+ +L+EM  VGL P+V ++++ + A A+  +  EALA+LK+M  + +  D   Y +AI A  K         L  EM AAG+TP+ ++Y++                                     I A  R G W+E + LL  M+   V P V++Y   M   A
Sbjct:  238 GLTPNVISYSSVITACAKEG----QWKIALDLLREMKAMRLAPNIITYNAAIDACAKGGRWKEAIDLLREMP-TVGLPPDVVSYSSVIDACSKGDRWKEAIDILREMPT-VGLSPNAISYNSAIDACAKGGQWKEAKGLLREMPTAGVTQRVIGYNSAIAACAKGEQWKEALALLREMPTVGLHTTVFSYSSAIDACGKGNLWIKAKELLREMATVGLAPNEVCYNSAIDACGRGDQWEEAVDLLREMPTVGLSPDVFSYSSAIAACAKGDQWKEALAVLKEMSAAGLAPDLICYNSAIDACSKGGRWKMAVALLGEMRAAGLTPNIISYSS------------------------------------AIDACVRGGQWKEGIALLEEMRGSGVVPDVITYHALMVTCA 604          

BLAST of EWM23818.1 vs. NCBI_GenBank
Match: gi|551540954|ref|XP_005758806.1| (hypothetical protein EMIHUDRAFT_53301, partial [Emiliania huxleyi CCMP1516] >gi|485606643|gb|EOD06377.1| hypothetical protein EMIHUDRAFT_53301, partial [Emiliania huxleyi CCMP1516])

HSP 1 Score: 172.17 bits (435), Expect = 1.092e-45
Identity = 115/377 (30.50%), Postives = 183/377 (48.54%), Query Frame = 0

Query:   44 TFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITAC---GRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
            + L ++   G  P+ +++N  +      G+   AL  LR M D  G+ PDV+S+   I+AC   GR  D A+ +L +   + GV+ D   Y+AA+  C  AGR  E L L                 +E  G G    +      +YNA + A +    W +A+ +L+EM   GL PNVV+++  + A  +AG   EAL+LL++M  + +  +  +Y AA  A  K+    +   L  EM  AG+ PD ++Y   +    +AG   +   L+++M    G   DV ++ A I A  +A  W +AL LL  M    V+P V+SY  A++A   + R  EA  LL  M    V+P+ +    A  +C  AGR ++ + L
Sbjct:   35 SLLREMPNAGVQPNVISYNAAISACEKAGRCDEALSLLREMPD-VGVQPDVISYNAAISACEKAGR-CDEALSLLREM-PDVGVQPDVISYSAAISACEKAGRCDEALSLL----------------REMPGAGVQPNE-----YSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAATSACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRCNEALSLLREMPSA-GVQPDVISYNAAISACEKASRWEKALSLLREMPDVGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSL 386          

HSP 2 Score: 154.836 bits (390), Expect = 3.185e-39
Identity = 109/376 (28.99%), Postives = 183/376 (48.67%), Query Frame = 0

Query:   45 FLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITAC---GRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTMKARNVKPTVVSYGTAMAALAASGRHVEAFELLEAMKREGVEPNQVVLEWATEACRTAGRLDKVVQL 417
             L ++ + G  P+  +++ ++   A       A+  LR M + AG+ P+V+S+   I+AC   GR  D A+ +L +   + GV+ D   Y AA+  C  AGR  E L L   M  +    +                     +I+Y+A + A +      +AL +L+EM   G+ PN  ++N  + A  +AG   EA++LL++M  + +  +  +Y+AAI A  K+    +   L  EM  AG++P+ ++Y        +AG  ++   L+++M    G   DV ++ A I A  +AG   EAL LL  M +  V+P V+SY  A++A   + R  +A  LL  M   GV+P+ +    A  AC  A R D+ + L
Sbjct:    1 ILDEMRQAGCEPNVFSYSILINLCARSKDWKTAVSLLREMPN-AGVQPNVISYNAAISACEKAGR-CDEALSLLREM-PDVGVQPDVISYNAAISACEKAGRCDEALSLLREMPDVGVQPD---------------------VISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAATSACEKAGRCDEALSLLREMP-GAGVQPDVISYNAAISACEKAGRCNEALSLLREMPSAGVQPDVISYNAAISACEKASRWEKALSLLREMPDVGVQPDVISYSAAISACEKASRCDEALSL 351          

HSP 3 Score: 132.109 bits (331), Expect = 5.911e-31
Identity = 105/358 (29.33%), Postives = 171/358 (47.77%), Query Frame = 0

Query:   14 GIVLDVKSYNALLPAVLAEAPPFLQQEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITAC---GRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQ-----ATQEKRKGGKERKGEGESEEDRLSVL------------IAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGITPDAVTYTTLMELYGQAGNKEQMEELVQQMSRKRGFPRDVRAFTAVIRAYGRAGLWREALELLGTM 351
            G+  +V SYNA + A         + +   + L ++   G  PD +++N  +      G+   AL  LR M D  G+ PDV+S++  I+AC   GR  D A+ +L +    +GV+ +   Y AA+  C  AGR  E + L   M A        +        E+ G  +   + LS+L            I+YNA   A +      +AL +L+EM   G+ P+V+++N  + A  +AG   EAL+LL++M  + V  D  +Y AAI A  K++   +   L  EM   G+ PD ++Y+  +    +A   ++   L+++M   R  P DV ++ A I +  +AG   EAL LL  M
Sbjct:   44 GVQPNVISYNAAISACEKAG----RCDEALSLLREMPDVGVQPDVISYNAAISACEKAGRCDEALSLLREMPD-VGVQPDVISYSAAISACEKAGR-CDEALSLLREMPG-AGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCD---EALSLLREMPDAGLSPNVISYNAATSACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRCNEALSLLREMPSAGVQPDVISYNAAISACEKASRWEKALSLLREMPDVGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQP-DVISYNAAISSCEKAGRCNEALSLLREM 390          

HSP 4 Score: 108.612 bits (270), Expect = 1.551e-22
Identity = 76/251 (30.28%), Postives = 124/251 (49.40%), Query Frame = 0

Query:   39 QEAVETFLGDLSRRGFAPDRVTFNTILKHHASMGKEALALETLRMMKDQAGIVPDVVSFTTVITAC---GRDWDGAMRVLAQAEQESGVEKDARMYTAAMRVCANAGRRREVLELFERMKAMQATQEKRKGGKERKGEGESEEDRLSVLIAYNAVLEALKTDKEWRQALQVLQEMRRVGLVPNVVNHNTVMDAIAEAGEAGEALALLKQMKVSRVPADKRTYTAAIKAMIKSADVLQGRELFEEMCAAGIT 286
            +EAV + L ++   G  P+ V+++  +      G+   AL  LR M D AG+ P+V+S+    +AC   GR  D A+ +L +    +GV+ D   Y AA+  C  AGR  E L L   M +     +                     +I+YNA + A +    W +AL +L+EM  VG+ P+V++++  + A  +A    EAL+LL++M  +RV  D  +Y AAI +  K+    +   L  EM  AG++
Sbjct:  171 EEAV-SLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPD-AGLSPNVISYNAATSACEKAGR-CDEALSLLREMPG-AGVQPDVISYNAAISACEKAGRCNEALSLLREMPSAGVQPD---------------------VISYNAAISACEKASRWEKALSLLREMPDVGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGLS 396