EWM24234.1, cds6441 (CDS) Nannochloropsis gaditana

Overview
NameEWM24234.1
Unique Namecds6441
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002468.1:546468..549362 -

Link to JBrowse

Properties
Property NameValue
Protein idEWM24234.1
Productpentatricopeptide repeat-containing protein
Orig transcript idgnl|cribi|Naga_100020g20.5367.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002468.1supercontigCM002468.1:546468..549362 -
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR001478PDZ
IPR002885Pentatricopeptide_repeat
IPR011990TPR-like_helical_dom
Homology
BLAST of EWM24234.1 vs. NCBI_GenBank
Match: gi|585105625|gb|EWM24234.1| (pentatricopeptide repeat-containing protein [Nannochloropsis gaditana])

HSP 1 Score: 1973.75 bits (5112), Expect = 0.000e+0
Identity = 964/964 (100.00%), Postives = 964/964 (100.00%), Query Frame = 0
Query:    1 METPPQTLPPSPPGNRHSPEPSRPQATSRIRRRSHSISGCGGAVIAEWWRLMLLSSSAATAAAAATAAFIGSGGATPTSVGGAVPTAWWVSTQQQQQLNRQSQLHNSWCAVSSTSRLATATGGWDSSAAPLRGRRGWDGMMGAPDKEEVLLRAASPVRGMSEKPQARWQGEGPDARDEEGQDRAGNAGSSWMEALRLEKGALRRNHRFEVTIQKPLGIFMSEGEADVGAPTISEVRLGSNADKAGLRVGDRIVAASATVGNKLWTKTTLVGVESAINSRFRFSDDITFCVERTLAQDAGDDSIASRQVEVFEVELERPVPLEWMQENAEGGVFVRRVLKADCEALRRIQPYDRVVAVSASLGDRMWRTNSLDGVLSALCTRIGSKVRLQFERSPEVARLIARGAGNPGVGVADLVPPLPPRPSMAAASLLSPPSTADLVAKGEMLLMGEPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLEMPTPSTGGAVSGLAHGDGVESQMEEAREQLVMFYHAALKLLAEQKVRARVNLYVACLHACLRTDRLDLAAEVMALRRANTLQIFQLFKKHEEKVHRWERKIDRELDARFYSNKNLV 964
            METPPQTLPPSPPGNRHSPEPSRPQATSRIRRRSHSISGCGGAVIAEWWRLMLLSSSAATAAAAATAAFIGSGGATPTSVGGAVPTAWWVSTQQQQQLNRQSQLHNSWCAVSSTSRLATATGGWDSSAAPLRGRRGWDGMMGAPDKEEVLLRAASPVRGMSEKPQARWQGEGPDARDEEGQDRAGNAGSSWMEALRLEKGALRRNHRFEVTIQKPLGIFMSEGEADVGAPTISEVRLGSNADKAGLRVGDRIVAASATVGNKLWTKTTLVGVESAINSRFRFSDDITFCVERTLAQDAGDDSIASRQVEVFEVELERPVPLEWMQENAEGGVFVRRVLKADCEALRRIQPYDRVVAVSASLGDRMWRTNSLDGVLSALCTRIGSKVRLQFERSPEVARLIARGAGNPGVGVADLVPPLPPRPSMAAASLLSPPSTADLVAKGEMLLMGEPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLEMPTPSTGGAVSGLAHGDGVESQMEEAREQLVMFYHAALKLLAEQKVRARVNLYVACLHACLRTDRLDLAAEVMALRRANTLQIFQLFKKHEEKVHRWERKIDRELDARFYSNKNLV
Sbjct:    1 METPPQTLPPSPPGNRHSPEPSRPQATSRIRRRSHSISGCGGAVIAEWWRLMLLSSSAATAAAAATAAFIGSGGATPTSVGGAVPTAWWVSTQQQQQLNRQSQLHNSWCAVSSTSRLATATGGWDSSAAPLRGRRGWDGMMGAPDKEEVLLRAASPVRGMSEKPQARWQGEGPDARDEEGQDRAGNAGSSWMEALRLEKGALRRNHRFEVTIQKPLGIFMSEGEADVGAPTISEVRLGSNADKAGLRVGDRIVAASATVGNKLWTKTTLVGVESAINSRFRFSDDITFCVERTLAQDAGDDSIASRQVEVFEVELERPVPLEWMQENAEGGVFVRRVLKADCEALRRIQPYDRVVAVSASLGDRMWRTNSLDGVLSALCTRIGSKVRLQFERSPEVARLIARGAGNPGVGVADLVPPLPPRPSMAAASLLSPPSTADLVAKGEMLLMGEPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLEMPTPSTGGAVSGLAHGDGVESQMEEAREQLVMFYHAALKLLAEQKVRARVNLYVACLHACLRTDRLDLAAEVMALRRANTLQIFQLFKKHEEKVHRWERKIDRELDARFYSNKNLV 964          
BLAST of EWM24234.1 vs. NCBI_GenBank
Match: gi|553184162|ref|XP_005854039.1| (hypothetical protein NGA_2025420 [Nannochloropsis gaditana CCMP526] >gi|553196310|ref|XP_005856315.1| hypothetical protein NGA_2025410 [Nannochloropsis gaditana CCMP526] >gi|422292736|gb|EKU20038.1| hypothetical protein NGA_2025410 [Nannochloropsis gaditana CCMP526] >gi|422295019|gb|EKU22318.1| hypothetical protein NGA_2025420 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.000e+0
Identity = 526/527 (99.81%), Postives = 527/527 (100.00%), Query Frame = 0
Query:  438 LVAKGEMLLMGEPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLEMPTPSTGGAVSGLAHGDGVESQMEEAREQLVMFYHAALKLLAEQKVRARVNLYVACLHACLRTDRLDLAAEVMALRRANTLQIFQLFKKHEEKVHRWERKIDRELDARFYSNKNLV 964
            +VAKGEMLLMGEPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLEMPTPSTGGAVSGLAHGDGVESQMEEAREQLVMFYHAALKLLAEQKVRARVNLYVACLHACLRTDRLDLAAEVMALRRANTLQIFQLFKKHEEKVHRWERKIDRELDARFYSNKNLV
Sbjct:    1 MVAKGEMLLMGEPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLEMPTPSTGGAVSGLAHGDGVESQMEEAREQLVMFYHAALKLLAEQKVRARVNLYVACLHACLRTDRLDLAAEVMALRRANTLQIFQLFKKHEEKVHRWERKIDRELDARFYSNKNLV 527          
BLAST of EWM24234.1 vs. NCBI_GenBank
Match: gi|553184168|ref|XP_005854041.1| (hypothetical protein NGA_2094920, partial [Nannochloropsis gaditana CCMP526] >gi|553196329|ref|XP_005856321.1| hypothetical protein NGA_2094910, partial [Nannochloropsis gaditana CCMP526] >gi|422292742|gb|EKU20044.1| hypothetical protein NGA_2094910, partial [Nannochloropsis gaditana CCMP526] >gi|422295021|gb|EKU22320.1| hypothetical protein NGA_2094920, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 735.332 bits (1897), Expect = 0.000e+0
Identity = 361/367 (98.37%), Postives = 363/367 (98.91%), Query Frame = 0
Query:    1 METPPQTLPPSPPGNRHSPEPSRPQATSRIRRRSHSISGCGGAVIAEWWRLMLLSSSAATAAAAATAAFIGSGGATPTSVGGAVPTAWWVSTQQQQQLNRQSQLHNSWCAVSSTSRLATATGGWDSSAAPLRGRRGWDGMMGAPDKEEVLLRAASPVRGMSEKPQARWQGEGPDARDEEGQDRAGNAGSSWMEALRLEKGALRRNHRFEVTIQKPLGIFMSEGEADVGAPTISEVRLGSNADKAGLRVGDRIVAASATVGNKLWTKTTLVGVESAINSRFRFSDDITFCVERTLAQDAGDDSIASRQVEVFEVELERPVPLEWMQENAEGGVFVRRVLKADCEALRRIQPYDRVVAVSASLGDRMWR 367
            METPPQTLPPSP GNRHSPEPSRPQATSRIRRRSHSISGCGGAVIAEWWRLMLLSSSAATAAAAATAAFIGSGGATPTSVGGAVPTAWWVSTQQQQQLNRQSQLHNSWCAVSSTSRLATATGGWDSSAAPLRGRRGWDGMMGAPDKEEVLLRAASPVRGMSEKPQARWQGEGPD R+EEGQDRAGN GSSWMEALRLEKGALRRNHRFEVTI+KPLGIFMSEGEADVGAPTISEVRLGSNADKAGLRVGDRIVAASATVGNKLWTKTTLVGVESAINSRFRFSDDITFCVERTLAQDAGDDSIASRQVEVFEVELERPVPLE MQENAEGGVFVRRVLKADCEALRRIQPYDRVVAVSASLGDRMWR
Sbjct:    1 METPPQTLPPSPLGNRHSPEPSRPQATSRIRRRSHSISGCGGAVIAEWWRLMLLSSSAATAAAAATAAFIGSGGATPTSVGGAVPTAWWVSTQQQQQLNRQSQLHNSWCAVSSTSRLATATGGWDSSAAPLRGRRGWDGMMGAPDKEEVLLRAASPVRGMSEKPQARWQGEGPDGREEEGQDRAGNVGSSWMEALRLEKGALRRNHRFEVTIRKPLGIFMSEGEADVGAPTISEVRLGSNADKAGLRVGDRIVAASATVGNKLWTKTTLVGVESAINSRFRFSDDITFCVERTLAQDAGDDSIASRQVEVFEVELERPVPLELMQENAEGGVFVRRVLKADCEALRRIQPYDRVVAVSASLGDRMWR 367          
BLAST of EWM24234.1 vs. NCBI_GenBank
Match: gi|1023176429|pdb|5I9F|A (Chain A, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U10 In Complex With Its Target Rna U10)

HSP 1 Score: 207.223 bits (526), Expect = 1.428e-54
Identity = 125/387 (32.30%), Postives = 208/387 (53.75%), Query Frame = 0
Query:  450 PRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRL 836
            P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  + + Y + +    R+ R+E A   LS + E  L
Sbjct:   49 PTVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDL 432          

HSP 2 Score: 206.838 bits (525), Expect = 1.470e-54
Identity = 121/378 (32.01%), Postives = 208/378 (55.03%), Query Frame = 0
Query:  471 AISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLE 848
            A+ +F     +G+ P     ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  +VV YN  + G  ++G+ + AL++   + EK + P+  T++ ++E
Sbjct:   35 ALELFAELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVE 409          

HSP 3 Score: 199.904 bits (507), Expect = 4.971e-52
Identity = 116/363 (31.96%), Postives = 200/363 (55.10%), Query Frame = 0
Query:  486 DAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLE 848
            D    ++++ A     + ++ALE+   +   GVAP +VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  +VV YN  + G  ++G+ + AL++   + EK + P++ T+  L++
Sbjct:   15 DVRAYTTVLHALSRAGRYERALELFAELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLID 374          

HSP 4 Score: 189.889 bits (481), Expect = 1.667e-48
Identity = 106/315 (33.65%), Postives = 176/315 (55.87%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRK 763
            +P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K
Sbjct:   83 KPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK 395          

HSP 5 Score: 150.984 bits (380), Expect = 3.265e-35
Identity = 88/264 (33.33%), Postives = 145/264 (54.92%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKA 712
            +P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP+   Y   V+ + +A
Sbjct:  153 KPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDELTYRRVVESYCRA 414          

HSP 6 Score: 135.961 bits (341), Expect = 2.966e-30
Identity = 83/256 (32.42%), Postives = 133/256 (51.95%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMR--DKGHVKPNLELY 702
            +P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD LTY  ++       + E A+    E+   D    K  LE Y
Sbjct:  188 KPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAY 442          
BLAST of EWM24234.1 vs. NCBI_GenBank
Match: gi|1023176433|pdb|5I9H|A (Chain A, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U8g2 In Complex With Its Target Rna U8g2)

HSP 1 Score: 206.453 bits (524), Expect = 2.194e-54
Identity = 125/387 (32.30%), Postives = 208/387 (53.75%), Query Frame = 0
Query:  450 PRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRL 836
            P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  YT +I    K   +  AL L   + + G+ PD   Y TL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  + + Y + +    R+ R+E A   LS + E  L
Sbjct:   49 PTVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDL 432          

HSP 2 Score: 204.912 bits (520), Expect = 8.434e-54
Identity = 120/378 (31.75%), Postives = 207/378 (54.76%), Query Frame = 0
Query:  471 AISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLE 848
            A+ +F     +G+ P     ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   Y TL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  +VV YN  + G  ++G+ + AL++   + EK + P+  T++ ++E
Sbjct:   35 ALELFAELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVE 409          

HSP 3 Score: 200.29 bits (508), Expect = 3.089e-52
Identity = 116/363 (31.96%), Postives = 200/363 (55.10%), Query Frame = 0
Query:  486 DAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLE 848
            D    ++++ A     + ++ALE+   +   GVAP +VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  +VV YN  + G  ++G+ + AL++   + EK + P++ T+  L++
Sbjct:   15 DVRAYTTVLHALSRAGRYERALELFAELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLID 374          

HSP 4 Score: 193.356 bits (490), Expect = 8.087e-50
Identity = 108/315 (34.29%), Postives = 178/315 (56.51%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRK 763
            +P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +YT L++   K G+L EAL LFEE V  G++P++  YT +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K
Sbjct:   83 KPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK 395          
BLAST of EWM24234.1 vs. NCBI_GenBank
Match: gi|1023176427|pdb|5I9D|A (Chain A, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U8a2 In Complex With Its Target Rna U8a2)

HSP 1 Score: 206.068 bits (523), Expect = 2.828e-54
Identity = 120/363 (33.06%), Postives = 200/363 (55.10%), Query Frame = 0
Query:  486 DAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLE 848
            D    ++++ A     + ++ALE+   +   GVAP +VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TYS L+  L    +L+ A  L+ EM +KG +KPN+  YS  +D   KAG   +A+ +F+EM    ++PN+VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  +VV YN  + G  ++G+ + AL++   + EK + P++ T+  L++
Sbjct:   15 DVRAYTTVLHALSRAGRYERALELFAELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYSTLIDGLCKAGKLDEALKLFEEMVEKG-IKPNVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLID 374          

HSP 2 Score: 206.068 bits (523), Expect = 3.306e-54
Identity = 125/397 (31.49%), Postives = 215/397 (54.16%), Query Frame = 0
Query:  452 VHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLE 848
            V A  + +    RA   + A+ +F     +G+ P     ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   Y+TL+ G  K  +  +A+++   M  KG+ P+ +TYS L+  L    +L+ A  L+ EM +KG +KPN+  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  +VV YN  + G  ++G+ + AL++   + EK + P+  T++ ++E
Sbjct:   16 VRAYTTVLHALSRAGRYERALELFAELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYSTLIDGLCKAGKLDEALKLFEEMVEKG-IKPNVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVE 409          

HSP 3 Score: 201.445 bits (511), Expect = 1.352e-52
Identity = 122/387 (31.52%), Postives = 209/387 (54.01%), Query Frame = 0
Query:  450 PRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRL 836
            P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y+ +I    K   +  AL L   + + G+ P+   Y+TL+ G  K  +  +A+++   M  KG+ P+ +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  + + Y + +    R+ R+E A   LS + E  L
Sbjct:   49 PTVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDL 432          

HSP 4 Score: 187.963 bits (476), Expect = 6.530e-48
Identity = 105/315 (33.33%), Postives = 177/315 (56.19%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRK 763
            +P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y+ L++   K G+L EAL LFEE V  G++P +  Y+ +I    K   +  AL L   + + G+ P+   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K
Sbjct:   83 KPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK 395          

HSP 5 Score: 145.976 bits (367), Expect = 1.488e-33
Identity = 86/264 (32.58%), Postives = 145/264 (54.92%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKA 712
            +P V    + + G  +A   D A+ +F+  VE+G+ PD    S+LI       + D+AL++   M+E G+ P++VT +  +D   K  + +E L+LF EE+   G+ P++ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP+   Y   V+ + +A
Sbjct:  153 KPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYSTLIDGLCKAGKLDEALKLF-EEMVEKGIKPNVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDELTYRRVVESYCRA 414          

HSP 6 Score: 133.65 bits (335), Expect = 1.773e-29
Identity = 82/256 (32.03%), Postives = 133/256 (51.95%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMR--DKGHVKPNLELY 702
            +P V    + + G  +A   D A+ +F+  VE+G+ P+    S+LI       + D+AL++   M+E G+ P++VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD LTY  ++       + E A+    E+   D    K  LE Y
Sbjct:  188 KPDVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAY 442          
BLAST of EWM24234.1 vs. NCBI_GenBank
Match: gi|1023176431|pdb|5I9G|C (Chain C, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U8c2 In Complex With Its Target Rna U8c2)

HSP 1 Score: 203.756 bits (517), Expect = 1.769e-53
Identity = 124/397 (31.23%), Postives = 214/397 (53.90%), Query Frame = 0
Query:  452 VHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLE 848
            V A  + +    RA   + A+ +F     +G+ P     ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ P  +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  +VV YN  + G  ++G+ + AL++   + EK + P+  T++ ++E
Sbjct:   16 VRAYTTVLHALSRAGRYERALELFAELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVE 409          

HSP 2 Score: 201.83 bits (512), Expect = 1.113e-52
Identity = 123/387 (31.78%), Postives = 206/387 (53.23%), Query Frame = 0
Query:  450 PRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRL 836
            P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ P    YNTL+ G  K  +  +A+++   M  KG+ P  +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  + + Y + +    R+ R+E A   LS + E  L
Sbjct:   49 PTVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDL 432          

HSP 3 Score: 199.134 bits (505), Expect = 9.340e-52
Identity = 116/363 (31.96%), Postives = 200/363 (55.10%), Query Frame = 0
Query:  486 DAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLE 848
            D    ++++ A     + ++ALE+   +   GVAP +VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K G+  DV +    +    + G L+    L +EM + GI  +VV YN  + G  ++G+ + AL++   + EK + P++ T+  L++
Sbjct:   15 DVRAYTTVLHALSRAGRYERALELFAELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEK-GIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLID 374          

HSP 4 Score: 186.037 bits (471), Expect = 2.836e-47
Identity = 105/315 (33.33%), Postives = 174/315 (55.24%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRK 763
            +P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ PD+VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P +  Y  +I    K   +  AL L   + + G+ P    YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP++  Y+  +D   KAG   +A+ +F+EM    ++P++VTY  L++   +AG    AL + +EM  K
Sbjct:   83 KPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEK 395          

HSP 5 Score: 145.206 bits (365), Expect = 2.285e-33
Identity = 86/264 (32.58%), Postives = 143/264 (54.17%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKA 712
            +P V    + + G  +A   D A+ +F+  VE+G+ PD    ++LI       + D+AL++   M+E G+ P +VT N  +D   K  + +E L+LF EE+   G+ P + +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD +TY+ L+  L    +L+ A  L+ EM +KG +KP+   Y   V+ + +A
Sbjct:  153 KPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKG-IKPDELTYRRVVESYCRA 414          

HSP 6 Score: 130.568 bits (327), Expect = 1.965e-28
Identity = 81/256 (31.64%), Postives = 131/256 (51.17%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMR--DKGHVKPNLELY 702
            +P V    + + G  +A   D A+ +F+  VE+G+ P     ++LI       + D+AL++   M+E G+ P +VT N  +D   K  + +E L+LF EE+   G+ PD+ +Y  L++   K G+L EAL LFEE V  G++P++  Y  +I    K   +  AL L   + + G+ PD   YNTL+ G  K  +  +A+++   M  KG+ PD LTY  ++       + E A+    E+   D    K  LE Y
Sbjct:  188 KPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLF-EEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAY 442          
BLAST of EWM24234.1 vs. NCBI_GenBank
Match: gi|1200537766|gb|OUW49982.1| (hypothetical protein CBD47_00860 [Synechococcus sp. TMED187])

HSP 1 Score: 207.608 bits (527), Expect = 6.492e-52
Identity = 225/828 (27.17%), Postives = 366/828 (44.20%), Query Frame = 0
Query:  208 FEVTIQKPLGIFMSEGEADVGAPTISEVRLGSNADKAGLRVGDRIVAASATVGNKLWTKTTLVGVESAINSRF------------------RFSDDITFCVERTLAQDAG---DDSIASRQVEVFEVELERPVPL-------------EWMQENAEGGVFVRRVLKADCEALR-RIQPYDRVVAVSASLGDRMWRTNSLDGVLSALCTR--IGSKVRLQFERSPEVARLIARGAGNPGVGVADLVPP---LPPRPSMAAASLLS-------------PPSTADLVAK----------GEMLLMGEPR-------VHAVLSQ----------------MAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAV----ACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIEN---LIQEMQDSG--IHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCL-LEMPTPSTGGAVSGLAHGDGVESQMEEAREQLVMFYHAALKLLAEQKVRARVNLYVACLHACLRTDRLDLAAEVMALRRANTLQIFQLFKKHE 939
            FEV  +KPLG+ + E         ++ V  GS A + G+R GD IVA SA++G+++W K TL GVESAI +R                   R+ D + +  E +L +  G     S  S  +     E   P P+             +   E    G  V  V++    A    ++  D VVA SAS+GD MWR +S++G+ +A+ TR  +   VRL+  RS  + R              +L PP    P R  ++AA+L S             P + AD + +          G M ++ +         V AVL +                M  ALRA D  LA+ +F   V+ G  P+A   ++LIKA+G+  Q  +A+ V++ M   GV P +   NAAM    +G     ++ LF   +   GL P + S+  ++ ++ K   L E   L EE +       + P+  +Y  + +A V++  +  A  L+  +  +G+  DA + NTLL G+ K+L W++A++    + + G   D L+YS LL   V   Q      +    R  G   PN  +Y+L +   A+ G+ ++A+ +F++M+ + V P   T+ ALMEA +    P  A ++  +M  K G+A DV +    L+          +E    L+++M   G     N + +N  L G  RS +++LAL +L  +   R+ P   T   L LE   P+   A +G     G  +    A E  + F    +++  E +      +Y A L  C RT  +  A  ++A R    L + +  +  E
Sbjct:   45 FEVACKKPLGLQLREKPGR--GVEVALVYEGSKAAQLGVRAGDLIVATSASLGSQMWEKKTLAGVESAIQTRVDGIVRLRLRRERAERWRSRWEDPVKYEYEVSLTRPIGLVLRPSSPSVALSAAATEAAAPEPVGAATAMGDSSAGGQSADERPPAGAEVAEVVEGGSAAASGAVRVGDVVVATSASVGDSMWRKSSVEGITAAISTRLSLSGSVRLRLSRSIRLGRW-----------AVELDPPPSGQPRRRRLSAAALSSYRQQLGELRSAPLPVALADAIRRICARAVRPPLGRMPIVADGERGADARAVCAVLRRLRSARVALDARLANGLMGTALRADDAQLALRVFDELVDGGGAPNAQVFTTLIKAHGVAGQRQEAVAVLQRMRAQGVPPTVRAYNAAMAVRARGGDRRGMMELF-AAISADGLRPTVASWNAVL-LFCKEQRLPE---LAEEVLRRMRRAAMTPDAFSYACVTQACVQRGRLERARELVDEMQAAGIPADAFILNTLLGGYAKQLRWTEALRTFNKLTSGGTAADLLSYSLLLRACVRARQPAQGAVVLERARAAGFA-PNARMYTLLLAAHAQEGEVSEALELFQQMQASGVPPTRHTFGALMEACIVVRQPAPAQALFAQM-EKAGIAADVVTHTLLLRALATHPTRSSLERGVALLEQMSAEGGDATPNALSFNALLDGCARSAQHDLALVLLRRMLRMRVSPTRRTFSHLALE---PAAMAAANGGGGAAGSSAGSTAAAESQLRFLLDVVEIFEEYRRPLGGAVYSALLRCCTRTGEVQTAEALVAKRHGGRLVVLRRAEMEE 849          
BLAST of EWM24234.1 vs. NCBI_GenBank
Match: gi|551540996|ref|XP_005758827.1| (hypothetical protein EMIHUDRAFT_53066, partial [Emiliania huxleyi CCMP1516] >gi|485606664|gb|EOD06398.1| hypothetical protein EMIHUDRAFT_53066, partial [Emiliania huxleyi CCMP1516])

HSP 1 Score: 180.644 bits (457), Expect = 1.128e-45
Identity = 119/370 (32.16%), Postives = 199/370 (53.78%), Query Frame = 0
Query:  471 AISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNL 840
            A+S+ +     G+ P+    ++ I A     + D+AL ++R M ++GV PD+++ +AA+ ACEK  R +E L L RE +P  G+ P+ +SY   ++   K G   EA++L  E  A GL P + +Y+A I A  K      AL+LLR + D+GLSP+   YN  +    K     +A+ +LR M   G+ PD ++Y+  +S      +   A SL REM   G V+P++  YS A+    KA    +A+++ +EM   RVQP++++Y A + +  +AG    ALS+  EM    GL+ +  S    +    + G      +L++EM  +G+  NV+ YN  +    ++GR + AL +LS + E  + P++
Sbjct:   64 ALSLLREMPGAGVQPNVISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYSAAISACEKAGRCDEALSLLRE-MPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSAG-VQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMP-DAGLSPNEYSYNAAISACEKAGRCNEALSLLREMPGAGLTPNVISYNAAISACEKAGRCDEALSLLSKMQEDGVQPDV 430          

HSP 2 Score: 169.859 bits (429), Expect = 5.997e-42
Identity = 112/363 (30.85%), Postives = 187/363 (51.52%), Query Frame = 0
Query:  482 GLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQ 844
            G  P+    S LI      K    A+ ++R M   GV P++++ NAA+  CEK  + ++ L L RE +P  G+ P++ SY   ++   K G   EAL+L  E    G++P++ +Y+A I A  K      AL+LLR +  +G+ P+   YN  +    K   W +A+ +LR M A GL P+ ++YS  +S      + + A SL REM D G + PN+  Y+ A+    KAG   +A+++ +EM    VQP++++Y A + A  +AG    ALS+  EM    G+  DV S    +    +    +   +L++EM  + +  +V+ YN  +    ++GR   AL +L  + +  L PN  ++ 
Sbjct:    5 GCEPNVFSYSILINLCARSKDWKTAVSLLREMPNAGVQPNVISYNAAISVCEKASQWKKALSLLRE-MPGAGVQPNVISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAG-LSPNVISYNAAISACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSA-GVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGLSPNEYSYN 364          

HSP 3 Score: 148.673 bits (374), Expect = 9.336e-35
Identity = 99/324 (30.56%), Postives = 170/324 (52.47%), Query Frame = 0
Query:  449 EPRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTS 772
            +P V +  + ++   +A   D A+S+ +     G+ P+ +  ++ I A     + ++A+ ++R M   G+ P++V+ +AA+ ACEK  R +E L L RE +P  GL P++ SY   ++   K G   EAL+L  E    G++P++ +Y A I A  K      AL+LLR +  +G+ PD   Y+  +    K     +A+ +LR M    + PD ++Y+  +S      +   A SL REM D G + PN   Y+ A+    KAG   +A+++ +EM    + PN+++Y A + A  +AG    ALS+  +M+   G+  DV S
Sbjct:  112 QPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLRE-MPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSAGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAG-LSPNEYSYNAAISACEKAGRCNEALSLLREMPGAGLTPNVISYNAAISACEKAGRCDEALSLLSKMQED-GVQPDVIS 432          

HSP 4 Score: 145.206 bits (365), Expect = 1.570e-33
Identity = 86/267 (32.21%), Postives = 147/267 (55.06%), Query Frame = 0
Query:  471 AISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTY 737
            A+S+ +     GL P+    S+ I A     + D+AL ++R M + G++P++++ NAA+ ACEK  R +E L L R E+P  G+ PD+ SY   ++   K G   EAL+L  E  + G++P++ +Y+A I A  K      AL+LLR +  + + PD   YN  +    K    ++A+ +LR M   GL P+  +Y+  +S      +   A SL REM   G + PN+  Y+ A+    KAG   +A+++  +M+ + VQP+++++
Sbjct:  169 AVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAAISACEKAGRCDEALSLLR-EMPGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSAGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGLSPNEYSYNAAISACEKAGRCNEALSLLREMPGAG-LTPNVISYNAAISACEKAGRCDEALSLLSKMQEDGVQPDVISF 433          

HSP 5 Score: 107.842 bits (268), Expect = 4.498e-21
Identity = 68/217 (31.34%), Postives = 111/217 (51.15%), Query Frame = 0
Query:  450 PRVHAVLSQMAGALRARDPDLAISIFKLAVERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTY 666
            P V +  + ++   +A   D A+S+ +     G+ PD    ++ I A     + ++AL ++R M   GV PD+++ +AA+ ACEK  R +E L L RE +P   + PD+ SY   ++   K G   EAL+L  E    GL P   +Y A I A  K      AL+LLR +  +GL+P+   YN  +    K     +A+ +L  M+  G+ PD +++
Sbjct:  218 PNVISYNAAISACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSAGVQPDVISYSAAISACEKASRCDEALSLLRE-MPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGLSPNEYSYNAAISACEKAGRCNEALSLLREMPGAGLTPNVISYNAAISACEKAGRCDEALSLLSKMQEDGVQPDVISF 433          

HSP 6 Score: 83.1889 bits (204), Expect = 4.835e-13
Identity = 57/190 (30.00%), Postives = 98/190 (51.58%), Query Frame = 0
Query:  654 MEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDLEGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETH 843
            M   G  P+  +YS L++        + A SL REM + G V+PN+  Y+ A+ +  KA    +A+++ +EM    VQPN+++Y A + A  +AG    ALS+  EM    G+  DV S    +    + G  +   +L++EM  +G+  N   YN  +    ++GR+E A+ +L  +    L PN+ ++
Sbjct:    1 MRQAGCEPNVFSYSILINLCARSKDWKTAVSLLREMPNAG-VQPNVISYNAAISVCEKASQWKKALSLLREMPGAGVQPNVISYNAAISACEKAGRCDEALSLLREM-PDVGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSY 188          
BLAST of EWM24234.1 vs. NCBI_GenBank
Match: gi|551664760|ref|XP_005835143.1| (hypothetical protein GUITHDRAFT_86178 [Guillardia theta CCMP2712] >gi|428179291|gb|EKX48163.1| hypothetical protein GUITHDRAFT_86178 [Guillardia theta CCMP2712])

HSP 1 Score: 181.8 bits (460), Expect = 6.417e-45
Identity = 137/478 (28.66%), Postives = 233/478 (48.74%), Query Frame = 0
Query:  483 LTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLF---REEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILRLMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGDTTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDEMRRKPGLARDVTSCVWRLKCFVELGDL-EGIENLIQEMQDSGIHLNVVVYNQYLRGAMRSGRYELALQILSALFEKRLLPNLETHQCLLEMPTPSTGGAVSGLAHGDGVESQMEEAREQLVMFYHA-ALKLLAEQKVRARVNLYVACLHACLRTDRLDLAAEVMALRRANTLQIFQLFKKHEEKVHRWERKIDRELDA 955
            L P  HC  +LIKAYG   Q + A E +  M++ G AP +  +NA + AC +         LF   R       L P   S+ ++VN Y +R ++ +A  +  E    G +P+   YT ++KA V       A  LL  + + G+ P+A  YN +L+G  ++L+W KA ++L+ ME  G+  + L+YSY +   V   +++ A +L+ EMR KG V PNL + +  +  +A  G   +A  I K ++  R++ N +T+++L+EAF+ AGY   A+ +  +M  + G+  D+      LK +  +G + E IE L + ++++   ++   ++  L    + G  E AL++L  +   R+ P+  T Q L                      S+ME+ +  ++  Y    + LL    VR    LY+A L A L     +LA  V+  R    L  F + K+ EE+  + E ++   L A
Sbjct:   29 LRPSVHCYGTLIKAYGQLGQLEAAFETLNGMVKGGPAPSIRVINALLQACVRAGNLTAAQTLFSSLRGAGLCISLQPTGVSFNIMVNAYARRRKVEKAFKMLLEMRRAGCKPDQITYTTLVKACVAAGQFDRAERLLGEMEELGIPPNAYSYNCILYGLAQKLQWGKATRLLQRMEEDGVEANLLSYSYCILACVRARRMKEASALFEEMRRKGIV-PNLFVMNTMMSGYAAKGMVDEAQGIMKLLQDMRIKANAMTFSSLIEAFINAGYADKAVEVKRKM-EELGVEVDMVVSTQMLKAYTSMGKVEEAIEKLEEIVKEASSPVSSRTFDSVLFQCSKRGSKEEALRVLGLMLRSRVTPSRTTCQLL----------------------SRMEKLQGSILTQYLLDVISLLRSHSVRPPGPLYLAVLRASLDAGETELATMVVEER---ILGKFSVSKQDEEEAEKLEMRMMNLLQA 479          

HSP 2 Score: 66.6254 bits (161), Expect = 1.510e-7
Identity = 66/284 (23.24%), Postives = 132/284 (46.48%), Query Frame = 0
Query:  480 ERGLTPDAHCCSSLIKAYGMKKQADKALEVVRMMLELGVAPDLVTLNAAMDACEKGKRPEEVLRLFREEVPRHGLVPDIFSYTVLVNMYGKRGELAEALALFEEAVACGLEPELPAYTAIIKAAVKKDDMGTALALLRRVFDSGLSPDARLYNTLLWGHTKRLEWSKAMQILR--LMEAKGLVPDRLTYSYLLSCLVDCNQLEVAQSLWREMRDKGHVKPNLELYSLAVDMFAKAGD--TTQAVTIFKEMRRNRVQPNLVTYTALMEAFVRAGYPMAALSISDE 759
            E G+ P+A+  + ++     K Q  KA  +++ M E GV  +L++ +  + AC + +R +E   LF EE+ R G+VP++F    +++ Y  +G + EA  + +      ++     ++++I+A +       A+ + R++ + G+  D  +   +L  +T   +  +A++ L   + EA   V  R   S L  C    ++ E  + L   +R +  V P+     L   M    G   T   + +   +R + V+P    Y A++ A + AG    A  + +E
Sbjct:  170 ELGIPPNAYSYNCILYGLAQKLQWGKATRLLQRMEEDGVEANLLSYSYCILACVRARRMKEASALF-EEMRRKGIVPNLFVMNTMMSGYAAKGMVDEAQGIMKLLQDMRIKANAMTFSSLIEAFINAGYADKAVEVKRKMEELGVEVDMVVSTQMLKAYTSMGKVEEAIEKLEEIVKEASSPVSSRTFDSVLFQCSKRGSKEEALRVLGLMLRSR--VTPSRTTCQLLSRMEKLQGSILTQYLLDVISLLRSHSVRPPGPLYLAVLRASLDAGETELATMVVEE 450          
The following BLAST results are available for this feature:
BLAST of EWM24234.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585105625|gb|EWM24234.1|0.000e+0100.00pentatricopeptide repeat-containing protein [Nanno... [more]
gi|553184162|ref|XP_005854039.1|0.000e+099.81hypothetical protein NGA_2025420 [Nannochloropsis ... [more]
gi|553184168|ref|XP_005854041.1|0.000e+098.37hypothetical protein NGA_2094920, partial [Nannoch... [more]
gi|1023176429|pdb|5I9F|A1.428e-5432.30Chain A, Crystal Structure Of Designed Pentatricop... [more]
gi|1023176433|pdb|5I9H|A2.194e-5432.30Chain A, Crystal Structure Of Designed Pentatricop... [more]
gi|1023176427|pdb|5I9D|A2.828e-5433.06Chain A, Crystal Structure Of Designed Pentatricop... [more]
gi|1023176431|pdb|5I9G|C1.769e-5331.23Chain C, Crystal Structure Of Designed Pentatricop... [more]
gi|1200537766|gb|OUW49982.1|6.492e-5227.17hypothetical protein CBD47_00860 [Synechococcus sp... [more]
gi|551540996|ref|XP_005758827.1|1.128e-4532.16hypothetical protein EMIHUDRAFT_53066, partial [Em... [more]
gi|551664760|ref|XP_005835143.1|6.417e-4528.66hypothetical protein GUITHDRAFT_86178 [Guillardia ... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna6457rna6457Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications