EWM24275.1, cds6421 (CDS) Nannochloropsis gaditana

Overview
NameEWM24275.1
Unique Namecds6421
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002468.1:513715..513726 -
Alignment locationCM002468.1:513570..513637 -
Alignment locationCM002468.1:512920..513313 -
Alignment locationCM002468.1:512690..512749 -

Link to JBrowse

Properties
Property NameValue
Protein idEWM24275.1
Productdna-directed rna polymerase ii 19 kda polypeptide
Orig transcript idgnl|cribi|Naga_100020g41.5408.mrna
GbkeyCDS
Noteencoded by transcript variant 1; alternatively spliced
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002468.1supercontigCM002468.1:513715..513726 -
CM002468.1supercontigCM002468.1:513570..513637 -
CM002468.1supercontigCM002468.1:512920..513313 -
CM002468.1supercontigCM002468.1:512690..512749 -
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Cellular Component
TermDefinition
GO:0005622intracellular
Vocabulary: Biological Process
TermDefinition
GO:0006139nucleobase-containing compound metabolic process
GO:0009058biosynthetic process
GO:0006351transcription, DNA-templated
Vocabulary: Molecular Function
TermDefinition
GO:0016740transferase activity
GO:0003676nucleic acid binding
GO:0003899DNA-directed 5'-3' RNA polymerase activity
Vocabulary: INTERPRO
TermDefinition
IPR003029Rbsml_prot_S1_RNA-bd_dom
IPR012340NA-bd_OB-fold
IPR005576RNA_pol_Rpb7_N
Homology
BLAST of EWM24275.1 vs. NCBI_GenBank
Match: gi|585105666|gb|EWM24275.1| (dna-directed rna polymerase ii 19 kda polypeptide [Nannochloropsis gaditana])

HSP 1 Score: 362.459 bits (929), Expect = 8.038e-127
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0
Query:    1 MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDYGFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISGETD 177
            MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDYGFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISGETD
Sbjct:    1 MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDYGFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISGETD 177          
BLAST of EWM24275.1 vs. NCBI_GenBank
Match: gi|585105667|gb|EWM24276.1| (dna-directed rna polymerase ii 19 kda polypeptide [Nannochloropsis gaditana])

HSP 1 Score: 298.516 bits (763), Expect = 6.338e-102
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0
Query:   33 MMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDYGFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISGETD 177
            MMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDYGFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISGETD
Sbjct:    1 MMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDYGFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISGETD 145          
BLAST of EWM24275.1 vs. NCBI_GenBank
Match: gi|325185644|emb|CCA20126.1| (DNAdirected RNA polymerase II 19 kDa polypeptide pu [Albugo laibachii Nc14])

HSP 1 Score: 169.859 bits (429), Expect = 1.250e-50
Identity = 81/179 (45.25%), Postives = 118/179 (65.92%), Query Frame = 0
Query:    1 MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDY--GFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISGETD 177
            MF+LK L +D +L P   GP L + I+ RL + +EGT     G+VI +  + D+ I  G I+  +G V   +++  +LFRPF+NE+LDA V+ V  +GFFAD GP+Q ++V++H MP D   G+D ++N W + D EVEIR GCGVR+R++GV I    +N +G+I DDY GLIS E +
Sbjct:    1 MFFLKQLSRDLLLHPMHFGPKLHDIIRLRLIEEVEGTSMGKYGYVITVTEVRDEDIGKGVIQDNSGYVCFNIRYRAILFRPFKNEVLDAVVSVVNQLGFFADVGPLQ-VFVSRHAMPTDLNNGYDHENNAWISDDREVEIRKGCGVRLRIMGVSIDVTEINAIGTIKDDYLGLISSENN 178          
BLAST of EWM24275.1 vs. NCBI_GenBank
Match: gi|301099953|ref|XP_002899067.1| (DNA-directed RNA polymerase II 19 kDa polypeptide [Phytophthora infestans T30-4] >gi|675169637|ref|XP_008893703.1| hypothetical protein PPTG_02059 [Phytophthora parasitica INRA-310] >gi|262104379|gb|EEY62431.1| DNA-directed RNA polymerase II 19 kDa polypeptide [Phytophthora infestans T30-4] >gi|566021744|gb|ETI44655.1| hypothetical protein F443_10664 [Phytophthora parasitica P1569] >gi|567959058|gb|ETK84638.1| hypothetical protein L915_10426 [Phytophthora parasitica] >gi|567987780|gb|ETL38075.1| hypothetical protein L916_10318 [Phytophthora parasitica] >gi|568016998|gb|ETL91175.1| hypothetical protein L917_10259 [Phytophthora parasitica] >gi|568047413|gb|ETM44494.1| hypothetical protein L914_10286 [Phytophthora parasitica] >gi|568107369|gb|ETN21999.1| hypothetical protein PPTG_02059 [Phytophthora parasitica INRA-310] >gi|570325330|gb|ETO73278.1| hypothetical protein F444_10770 [Phytophthora parasitica P1976] >gi|570949236|gb|ETP14493.1| hypothetical protein F441_10611 [Phytophthora parasitica CJ01A1] >gi|570982404|gb|ETP42537.1| hypothetical protein F442_10571 [Phytophthora parasitica P10297] >gi|953498352|emb|CEG39990.1| rna polymerase ii subunit [Plasmopara halstedii] >gi|970655944|gb|KUF94025.1| Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family [Phytophthora nicotianae] >gi|970665100|gb|KUG01842.1| DNA-directed RNA polymerase II 19 kDa polypeptide [Phytophthora nicotianae])

HSP 1 Score: 164.081 bits (414), Expect = 1.794e-48
Identity = 78/178 (43.82%), Postives = 116/178 (65.17%), Query Frame = 0
Query:    1 MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDY--GFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISGET 176
            MF+LK L +D +L P   GP L + I+ RL + +EGT     G+VI +  + D+ I  G I+  +G V   +++  +LFRPF+N++LDA VT V  +GFFAD GP+Q ++V++H MP D   G+D + N W + D EVEIR GCGVR++++GV +    +N +G+I DDY GLIS + 
Sbjct:    1 MFFLKQLSRDLLLHPMHFGPKLHDIIRLRLIEEVEGTSMGKYGYVITVTEVRDEDIGKGVIQDNSGFVCFNIRYRAILFRPFKNQVLDAVVTVVNQLGFFADVGPLQ-VFVSRHAMPTDLNNGYDHESNAWISDDREVEIRKGCGVRLKIMGVSVDVTEINAIGTIKDDYLGLISSDN 177          
BLAST of EWM24275.1 vs. NCBI_GenBank
Match: gi|224002987|ref|XP_002291165.1| (19kDa subunit of RNA polymerase [Thalassiosira pseudonana CCMP1335] >gi|220972941|gb|EED91272.1| 19kDa subunit of RNA polymerase [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 159.458 bits (402), Expect = 1.761e-46
Identity = 80/182 (43.96%), Postives = 117/182 (64.29%), Query Frame = 0
Query:    1 MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDY--GFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLI---SGETD 177
            MFY+KTLE +  LEP   G  L+ TI R L + +EG    + GFV+ ++ + ++ IR G IEY+TG V  +VK+T +L RPF NE++DA V    ++GFFA  GP++ I+V+KH MP+D   GFD + + W + D EV I  GCGVR+++ G  +  G +  +G+I D+Y GLI   SGE +
Sbjct:    1 MFYVKTLEHEIELEPIHFGQKLRATIVRLLKEEVEGLALANYGFVVNVIEVPEEQIRSGIIEYDTGNVVFYVKYTALLLRPFINEVVDATVAQCNELGFFAYVGPLR-IFVSKHSMPEDMFNGFDGERDSWVSDDKEVVIASGCGVRLKIKGRTVEQGNITAIGTIKDNYLGLIDDGSGEAE 181          
BLAST of EWM24275.1 vs. NCBI_GenBank
Match: gi|1072235217|gb|OEU19946.1| (hypothetical protein FRACYDRAFT_181715 [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 158.688 bits (400), Expect = 3.523e-46
Identity = 78/179 (43.58%), Postives = 115/179 (64.25%), Query Frame = 0
Query:    1 MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDY--GFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISGETD 177
            MFY+KTLE + +L+P   GP LK TI R L + +EG    + GFV+ ++ + +  I+ G IEY+TG+V   VK+T +L RPF NE+LDA V+    +GFFA  GP++ ++V+KH MP+D   G++   + W + D EV I  GCGVR+R+ G  +  G +  +G+I D Y GL+S E D
Sbjct:    1 MFYVKTLEHEIVLQPSHFGPKLKPTIIRLLKEEVEGLALAEYGFVVNVIEVPEDQIKSGIIEYDTGDVVFSVKYTALLLRPFVNEVLDAVVSQCNTLGFFAYVGPLR-VFVSKHSMPEDMLNGWNTDKDSWVSDDKEVIISSGCGVRLRIKGRTVEQGNITAIGTIKDSYLGLVSEEDD 178          
BLAST of EWM24275.1 vs. NCBI_GenBank
Match: gi|298710882|emb|CBJ26391.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 158.303 bits (399), Expect = 4.314e-46
Identity = 80/177 (45.20%), Postives = 119/177 (67.23%), Query Frame = 0
Query:    1 MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDY--GFDAQDNVWRTS-DMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISG 174
            MFYLK L +  ++ P D GP LK+TI+R+L + +EGT    LGFVI +  + D  I+ G IE++TG   V V +  + FRPF NE+LDA V+ VT++GFFA+ GP++ ++V++  MP D   G+D   ++W +S D + EI+ GCGVR+R++GV +    +  VGSI DD+ GL+SG
Sbjct:    1 MFYLKELRRPMLVPPSDFGPKLKDTIRRKLIEEVEGTSVGSLGFVITVTNVSDDQIQMGTIEFDTGFANVLVVYRAICFRPFVNEVLDAAVSHVTELGFFAEVGPLE-VFVSRLGMPDDIAGGYDPVGDMWVSSEDSDTEIKSGCGVRLRIIGVTVG-AELKAVGSIKDDFLGLVSG 175          
BLAST of EWM24275.1 vs. NCBI_GenBank
Match: gi|635360330|emb|CCI50307.1| (unnamed protein product [Albugo candida])

HSP 1 Score: 164.851 bits (416), Expect = 8.360e-46
Identity = 78/179 (43.58%), Postives = 117/179 (65.36%), Query Frame = 0
Query:    1 MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDY--GFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLISGETD 177
            MF+LK L +D +L P   GP L + I+ RL + +EGT     G+VI +  + D+ I  G I+  +G V   +++  +LFRPF+N++LDA V+ V  +GFFAD GP+Q ++V++H MP D   G+D ++N W + D EVEIR  CG+R+R++GV I    +N +G+I DDY GLIS E +
Sbjct:  265 MFFLKQLSRDLLLHPMHFGPKLHDIIRLRLIEEVEGTSMGKYGYVITVTEVRDEDIGKGVIQDSSGYVCFNIRYRAILFRPFKNQVLDAVVSVVNQLGFFADVGPLQ-VFVSRHAMPTDLNNGYDHENNAWISDDREVEIRKSCGIRLRIMGVSIDVTEINAIGTIKDDYLGLISTENN 442          
BLAST of EWM24275.1 vs. NCBI_GenBank
Match: gi|219118585|ref|XP_002180062.1| (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >gi|217408319|gb|EEC48253.1| predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 156.377 bits (394), Expect = 2.264e-45
Identity = 76/174 (43.68%), Postives = 113/174 (64.94%), Query Frame = 0
Query:    1 MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQD--YGFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLI 172
            MFY+KTL+ + +LEP   GP LK TI R L + +EG    + G+V+ ++ + +  IR G IEY+TG V   VK+T +L RPF NE+LDA VT    +GFFA  GP++ I+V+KH MP+D  +GF+ + + W + D EV I  G GVR+++ G  +  G +  +G+I D+Y GL+
Sbjct:    1 MFYVKTLDHEIVLEPVHFGPKLKATIVRLLKEHVEGLALANYGYVVNVIEVPEDQIRSGIIEYDTGNVVFTVKYTALLLRPFLNEVLDAVVTQCNALGFFAFCGPLR-IFVSKHSMPEDMFHGFNHEQDAWVSDDKEVVIAAGSGVRLKIKGRTVEQGNITAIGTIKDNYLGLV 173          
BLAST of EWM24275.1 vs. NCBI_GenBank
Match: gi|397575684|gb|EJK49830.1| (hypothetical protein THAOC_31253 [Thalassiosira oceanica])

HSP 1 Score: 155.221 bits (391), Expect = 8.414e-45
Identity = 75/174 (43.10%), Postives = 112/174 (64.37%), Query Frame = 0
Query:    1 MFYLKTLEKDFMLEPKDLGPGLKETIKRRLTQMMEGTLEPDLGFVIALVALDDQHIRPGKIEYETGEVEVWVKFTVMLFRPFRNEILDAQVTTVTDMGFFADAGPVQGIYVNKHQMPQDY--GFDAQDNVWRTSDMEVEIRPGCGVRMRVVGVDITPGRMNVVGSIMDDYTGLI 172
            MFY+K LE +  LEP   G  LK+TI R L + +EG    + GFV+ ++ + ++ IR G IEY+TG V  +VK+T ++ RPF NE++DA V    ++GFFA  GP++ I+V+KH MP+D   GFD + + W + D EV I  GCGVR+++ G  +    +  +G+I D+Y GLI
Sbjct:    1 MFYVKMLEHEIELEPIHFGQKLKDTIVRLLKEEVEGLALANYGFVVNVIEVPEEQIRSGVIEYDTGNVVFYVKYTALMLRPFVNEVIDATVAQCNELGFFAYVGPLR-IFVSKHSMPEDMFNGFDGERDSWVSDDKEVVISSGCGVRLKIKGRTVEQNNITAIGTIKDNYLGLI 173          
The following BLAST results are available for this feature:
BLAST of EWM24275.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585105666|gb|EWM24275.1|8.038e-127100.00dna-directed rna polymerase ii 19 kda polypeptide ... [more]
gi|585105667|gb|EWM24276.1|6.338e-102100.00dna-directed rna polymerase ii 19 kda polypeptide ... [more]
gi|325185644|emb|CCA20126.1|1.250e-5045.25DNAdirected RNA polymerase II 19 kDa polypeptide p... [more]
gi|301099953|ref|XP_002899067.1|1.794e-4843.82DNA-directed RNA polymerase II 19 kDa polypeptide ... [more]
gi|224002987|ref|XP_002291165.1|1.761e-4643.9619kDa subunit of RNA polymerase [Thalassiosira pse... [more]
gi|1072235217|gb|OEU19946.1|3.523e-4643.58hypothetical protein FRACYDRAFT_181715 [Fragilario... [more]
gi|298710882|emb|CBJ26391.1|4.314e-4645.20conserved unknown protein [Ectocarpus siliculosus][more]
gi|635360330|emb|CCI50307.1|8.360e-4643.58unnamed protein product [Albugo candida][more]
gi|219118585|ref|XP_002180062.1|2.264e-4543.68predicted protein [Phaeodactylum tricornutum CCAP ... [more]
gi|397575684|gb|EJK49830.1|8.414e-4543.10hypothetical protein THAOC_31253 [Thalassiosira oc... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna6437rna6437Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications