EWM30090.1, cds584 (CDS) Nannochloropsis gaditana

Overview
NameEWM30090.1
Unique Namecds584
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002455.1:1459948..1459950 +
Alignment locationCM002455.1:1460240..1460368 +
Alignment locationCM002455.1:1460605..1461174 +

Link to JBrowse

Properties
Property NameValue
Protein idEWM30090.1
ProductPyridine nucleotide-disulfide oxidoreductase, NAD-binding domain protein
Orig transcript idgnl|cribi|Naga_100729g2.10074.mrna
GeneNDE1
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002455.1supercontigCM002455.1:1459948..1459950 +
CM002455.1supercontigCM002455.1:1460240..1460368 +
CM002455.1supercontigCM002455.1:1460605..1461174 +
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0000166nucleotide binding
GO:0050660flavin adenine dinucleotide binding
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR001327Pyr_OxRdtase_NAD-bd_dom
Homology
BLAST of EWM30090.1 vs. NCBI_GenBank
Match: gi|585112730|gb|EWM30090.1| (Pyridine nucleotide-disulfide oxidoreductase, NAD-binding domain protein [Nannochloropsis gaditana])

HSP 1 Score: 478.789 bits (1231), Expect = 5.974e-171
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0
Query:    1 MVSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVEPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSLSKGPGGRLLVDGRLRLQGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 233
            MVSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVEPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSLSKGPGGRLLVDGRLRLQGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF
Sbjct:    1 MVSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVEPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSLSKGPGGRLLVDGRLRLQGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 233          
BLAST of EWM30090.1 vs. NCBI_GenBank
Match: gi|553185261|ref|XP_005854223.1| (NADH dehydrogenase [Nannochloropsis gaditana CCMP526] >gi|422294837|gb|EKU22137.1| NADH dehydrogenase [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 430.639 bits (1106), Expect = 2.574e-149
Identity = 211/233 (90.56%), Postives = 211/233 (90.56%), Query Frame = 0
Query:    1 MVSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVEPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSLSKGPGGRLLVDGRLRLQGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 233
            MVSVTLIEASDHILGSFDRRLVDYV                    EPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPS  KGPGGRLLVDGRLRLQGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF
Sbjct:  191 MVSVTLIEASDHILGSFDRRLVDYV--------------------EPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSFPKGPGGRLLVDGRLRLQGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 403          
BLAST of EWM30090.1 vs. NCBI_GenBank
Match: gi|929752801|ref|XP_014151383.1| (hypothetical protein SARC_10059, partial [Sphaeroforma arctica JP610] >gi|906945278|gb|KNC77481.1| hypothetical protein SARC_10059, partial [Sphaeroforma arctica JP610])

HSP 1 Score: 197.593 bits (501), Expect = 6.002e-58
Identity = 105/243 (43.21%), Postives = 145/243 (59.67%), Query Frame = 0
Query:    2 VSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVE----------PNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPS-LSKGPGGRLLVDGRLRLQGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 233
             ++TL+EA+D ILG FD  L  YV R+F +R++++ T T+V  VE             A + DGT   FG  VWS G++       +    + +   GR+++D  LR++GH+ K V+A+GDCA  ET PLP LAQVAQQQ+ Y+  VL K     + ++   F +  LGSM  +G WKG+ D   L D  G    K + ++G+ AF+ +R AYW K VSW NK+LIPMYW KSW FGRDI RF
Sbjct:  155 TTLTLVEAADAILGPFDNHLRAYVERLFNKRNIKIRTKTAVTGVELCHVEGFHHESTKAIMSDGTEHRFGTLVWSAGLQPVKFTDKVTSKGIQRTEAGRIIIDEYLRVKGHEGK-VWAIGDCAECETMPLPLLAQVAQQQSGYMAKVLTK----SIREDEKAFHFYSLGSMLSVGKWKGIYDGQSLGDPYGWR-AKVTNISGFAAFITYRTAYWGKQVSWTNKLLIPMYWFKSWAFGRDICRF 391          
BLAST of EWM30090.1 vs. NCBI_GenBank
Match: gi|954188105|gb|KRX00931.1| (hypothetical protein PPERSA_09537 [Pseudocohnilembus persalinus])

HSP 1 Score: 194.126 bits (492), Expect = 4.825e-56
Identity = 101/246 (41.06%), Postives = 157/246 (63.82%), Query Frame = 0
Query:    2 VSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKV---------EPN-VAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSLSKGPGGRLLVDGRLRLQGHDQKG----VFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 233
            V +T++EA++ ILG+F++ L  YV ++F++RH+ + T+ + EK+         EP  V +L +G  + FG+ +WS GV+   L++     LSK   GR+ ++G+L+++ + ++G    VFA+GDCA ++ + LP +AQVA QQA+YL    N        K    FE+ +LG MA +G   GV+D+  + +  G +  K + L G++AFL+WRAAYW K VSW+NKILIPM+WLKS IFGRD S +
Sbjct:  218 VKITMVEAAEDILGTFNQNLRKYVEKLFKKRHINLKTEVAFEKIAEEKFDGFREPQPVGYLNNGEKMKFGMMIWSAGVRPIKLIRD-NEQLSKNKQGRIDINGQLQVKLNGKQGFEQDVFAIGDCATNKDKSLPQIAQVASQQARYLSKYFNNDC-----KSANDFEFFNLGQMAFIGNKSGVIDATSIGNPYGKKH-KITYLEGFSAFLIWRAAYWGKQVSWSNKILIPMHWLKSSIFGRDTSSY 456          
BLAST of EWM30090.1 vs. NCBI_GenBank
Match: gi|470412200|ref|XP_004336447.1| (NADH dehydrogenase, putative [Acanthamoeba castellanii str. Neff] >gi|440793246|gb|ELR14434.1| NADH dehydrogenase, putative [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 188.734 bits (478), Expect = 9.840e-54
Identity = 102/232 (43.97%), Postives = 143/232 (61.64%), Query Frame = 0
Query:    2 VSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVEPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSLSKGPGGRLLVDGRLRLQGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 233
            V +TLIEA   +L +FD+RL DY  R F++R++ V T  SV++V+ +   L DG  + FGL VWSTG+   P ++ LP    K   GRLLVD  L ++    +GV+AVGDCA  ET PLP  AQ A+Q+ KYL   LN  +     +E   F+Y H G +A +G ++ ++DS  +         K+S   G+  +++W AAY TK VS  NK++IPMYW KS++FGRDISRF
Sbjct:  259 VQITLIEAGKTLLSTFDQRLSDYTMRTFRKRNIDVRTSVSVKQVKRHEMVLSDGAVIPFGLGVWSTGLSPIPFIKGLP--FPKDRSGRLLVDEYLHVKAPGVEGVYAVGDCAAFETNPLPATAQGAEQEGKYLAQALNAKAR---GEEPKKFQYHHKGMLAYVGGYRALIDSPLI---------KRS---GFLTWIMWNAAYITKLVSIKNKMMIPMYWFKSFVFGRDISRF 473          
BLAST of EWM30090.1 vs. NCBI_GenBank
Match: gi|1023268447|gb|KZT63442.1| (FAD/NAD(P)-binding domain-containing protein [Daedalea quercina L-15889])

HSP 1 Score: 181.8 bits (460), Expect = 4.682e-51
Identity = 97/234 (41.45%), Postives = 139/234 (59.40%), Query Frame = 0
Query:    4 VTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVEPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSLSKGPGGRLLVDGRLRL----QGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 233
            ++L + +  ILGSFD+ L  Y  + FQ   +R+LT   VE+VEP   ++K+   +HFGL VWSTG+   PLV+++  +        L+ DG L +     GH    V+A+GD A+ +  PLP  AQVA Q+AKYL   LN   +     +  PF + + GS+A +G W+ V D  + +  KG E G+        A+LLWR+AY+T ++SW NKIL+P YW  +WIFGRDISRF
Sbjct:  264 ISLYDVAPQILGSFDKGLQAYATQKFQREGIRLLTQHHVERVEPGKMYVKEQGEVHFGLLVWSTGLAPNPLVESITETEKDPKTKTLITDGHLNVIMKDTGHPDPDVWAIGDAAMIKEMPLPATAQVANQKAKYLWKKLNALGKGRPAGD--PFRFHNAGSLAYVGDWQAVYDRTRAEKAKGKEAGR-------FAWLLWRSAYFTMTLSWKNKILVPTYWFMNWIFGRDISRF 488          
BLAST of EWM30090.1 vs. NCBI_GenBank
Match: gi|857970399|emb|CEP03422.1| (hypothetical protein PBRA_003182 [Plasmodiophora brassicae])

HSP 1 Score: 181.03 bits (458), Expect = 6.965e-51
Identity = 113/260 (43.46%), Postives = 143/260 (55.00%), Query Frame = 0
Query:    2 VSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVEPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSLSKGPGGRLLVDGRLRLQGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSE---PHVNKE----VPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMY---------------------WLKSWIFGRDISRF 233
            + +T++EA   ILGSFDR L +Y  R+FQ R + V T  +V++V      L DG+ +   L VWSTGV A PL+Q+L     K   GRLLV   L +      GV+A+GDCA++E  PLPC AQVAQQQAKYL  V N+Y+    P V  +      PF YRHL S+A +G WK + DS  +         K S L  W   L WR+AYWT +VS  NKILIPM                      W K+W+FGRDIS F
Sbjct:  220 IRITVLEAGRTILGSFDRALSEYTVRLFQARDIEVRTGVAVKQVTKRGVELGDGSVVPSNLVVWSTGVAAIPLIQSLNWEHDK-RSGRLLVSEYLSVP--SAPGVYALGDCAVNEKEPLPCTAQVAQQQAKYLARVFNQYNSVGGPPVGSQETTKTKPFVYRHLASLAYVGGWKSIADSKVV---------KSSGLGTW---LFWRSAYWTTNVSIRNKILIPMCKAMPACLVAVRLLTNIAWRIDWFKAWLFGRDISTF 464          
BLAST of EWM30090.1 vs. NCBI_GenBank
Match: gi|909127146|gb|KNE54457.1| (hypothetical protein AMAG_00431 [Allomyces macrogynus ATCC 38327])

HSP 1 Score: 180.259 bits (456), Expect = 1.719e-50
Identity = 101/237 (42.62%), Postives = 140/237 (59.07%), Query Frame = 0
Query:    2 VSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVEPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSLSKGPGG--RLLVDGRLRL---QGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 233
            V +TL + +  ILG+FD +L +Y  + F    V++ T T V  VE +   L DG  + +GL VW+TG+  T LV+ L   ++  P G  R++ D RLR+   QG   + V+A+GDCA      LP  AQVA Q+A YLG  LNK  E    K + PF YRHLGSMA +G W+ VVD           D K S   G++A++ WR++Y + SVSW NK LIP+YW  +++FGRD+SR 
Sbjct:  259 VRMTLYDVAPRILGAFDAKLAEYATKRFVRSGVQIRTGTQVRNVEKDKIVLNDGEEVPYGLLVWATGITQTELVRGLRGRVAPDPRGAHRIVTDRRLRVLDPQGEPMENVYALGDCATISDYDLPATAQVANQKALYLGKQLNKTDE----KAIVPFAYRHLGSMAYVGGWRAVVDMPN--------DIKPS---GYSAWIFWRSSYLSMSVSWRNKCLIPLYWFAAYVFGRDVSRI 480          
BLAST of EWM30090.1 vs. NCBI_GenBank
Match: gi|1183517063|gb|ORZ40279.1| (pyridine nucleotide-disulfide oxidoreductase-domain-containing protein [Catenaria anguillulae PL171])

HSP 1 Score: 178.718 bits (452), Expect = 9.094e-50
Identity = 106/238 (44.54%), Postives = 137/238 (57.56%), Query Frame = 0
Query:    2 VSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVEPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSL---SKGPGGRLLVDGRLRL---QGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSEPHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 233
            V +TL + +  ILG+FD  L     + F  R ++V T T V KVEP    L  G  + FGL VW+TG+  TPLV++L   L   +KG  GR++ D  LR     G     VFA+GDCA+ +   LP  AQVA Q+A YL   LNK   P     + PF Y+HLGSMA +G W+ V D +K        + K S   GW A++ WR+AY T SVSW NK LIPM+W  +W FGRD+SRF
Sbjct:  268 VQITLYDVAPRILGAFDASLSSEATKRFMRRGIKVRTGTRVAKVEPGTIVLASGERVPFGLLVWATGITQTPLVRSLADRLMAETKG-LGRIVTDDNLRALKPDGTPLPDVFALGDCAMIKGSDLPATAQVANQKAVYLAKQLNKPEAP-----MQPFAYKHLGSMAYIGGWRAVADISK--------EFKPS---GWGAWVFWRSAYLTMSVSWRNKALIPMFWFLAWAFGRDVSRF 488          
BLAST of EWM30090.1 vs. NCBI_GenBank
Match: gi|1183361170|gb|ORX88473.1| (FAD/NAD-P-binding domain-containing protein [Basidiobolus meristosporus CBS 931.73])

HSP 1 Score: 178.333 bits (451), Expect = 1.138e-49
Identity = 94/239 (39.33%), Postives = 137/239 (57.32%), Query Frame = 0
Query:    2 VSVTLIEASDHILGSFDRRLVDYVGRVFQERHVRVLTDTSVEKVEPNVAWLKDGTPLHFGLCVWSTGVKATPLVQALPPSLSKGPGGRLLVDGRLR----LQGHDQKGVFAVGDCAISETRPLPCLAQVAQQQAKYLGGVLNKYSE---PHVNKEVPPFEYRHLGSMAQLGMWKGVVDSAKLDDKKGDEDGKKSVLTGWTAFLLWRAAYWTKSVSWANKILIPMYWLKSWIFGRDISRF 233
            V +T+ + +  IL +FD  L  YV + F  R +++ T   +E+V P+   +K     H GL VW+TG+   PL++A+   ++K    RL+ D   R    L     K V+A+GDCA  +   LP  AQVA+Q+ +YL   LNK +    P++ +E+ PF+YRH  SMA +G WK +V+        G +        GW ++L WR+AY+T SVS  NK+LIPMYW  +WIFGRD SRF
Sbjct:  251 VQMTIYDVAPTILSNFDSSLEQYVTKRFMRRGIKIRTGAIIEEVCPDSIKIKGEGEKHTGLVVWATGLTENPLIKAMEGLVAKDGRKRLITDRHFRILDPLTQDPIKNVYAIGDCATIKDYDLPATAQVAKQKGEYLVKALNKIAREGSPNIVEEIKPFDYRHFASMAYVGGWKAIVEV-------GSDKAHDMKYAGWMSWLFWRSAYFTMSVSNKNKVLIPMYWFLTWIFGRDTSRF 482          
The following BLAST results are available for this feature:
BLAST of EWM30090.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585112730|gb|EWM30090.1|5.974e-171100.00Pyridine nucleotide-disulfide oxidoreductase, NAD-... [more]
gi|553185261|ref|XP_005854223.1|2.574e-14990.56NADH dehydrogenase [Nannochloropsis gaditana CCMP5... [more]
gi|929752801|ref|XP_014151383.1|6.002e-5843.21hypothetical protein SARC_10059, partial [Sphaerof... [more]
gi|954188105|gb|KRX00931.1|4.825e-5641.06hypothetical protein PPERSA_09537 [Pseudocohnilemb... [more]
gi|470412200|ref|XP_004336447.1|9.840e-5443.97NADH dehydrogenase, putative [Acanthamoeba castell... [more]
gi|1023268447|gb|KZT63442.1|4.682e-5141.45FAD/NAD(P)-binding domain-containing protein [Daed... [more]
gi|857970399|emb|CEP03422.1|6.965e-5143.46hypothetical protein PBRA_003182 [Plasmodiophora b... [more]
gi|909127146|gb|KNE54457.1|1.719e-5042.62hypothetical protein AMAG_00431 [Allomyces macrogy... [more]
gi|1183517063|gb|ORZ40279.1|9.094e-5044.54pyridine nucleotide-disulfide oxidoreductase-domai... [more]
gi|1183361170|gb|ORX88473.1|1.138e-4939.33FAD/NAD-P-binding domain-containing protein [Basid... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna584rna584Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications