EWM24946.1, cds5737 (CDS) Nannochloropsis gaditana

BLAST of EWM24946.1 vs. NCBI_GenBank
Match: gi|585106576|gb|EWM24946.1| (hypothetical protein Naga_100086g9 [Nannochloropsis gaditana])

HSP 1 Score: 1742.24 bits (4511), Expect = 0.000e+0
Identity = 847/847 (100.00%), Postives = 847/847 (100.00%), Query Frame = 0

Query:    1 MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKRRLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHPPSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDLEAFQALYNCAQVLHALRASLRTVLMVVVYVHVDQNGYESFSPDPSEWEDVCRLDAGWGRTGTGRAATGDKGVNDASYQGANRSDGDDSDEEQDGEEDEANEGQCQSSPCPIFLPVGVPNLPRGAAIEFEVVALSTAFAQVKSPTRRAEQGRLEDILEEKDMGECPLPLPFQPPATFAALASKNMPLMSRPLLSYTWEAIFVPYVFNFVSLSLYLDDDLDGRNEGGQNETLLLNYTHVAYALVDQLRKILRVAHLTWQQLSHLRVFYIATCLDNEAMLREALAAALPSDGQCARTFLPVSSLMSECQEGSKRQDSLFLRVQVIALDLEKLRTEAWVHSVPDGGM 847
            MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKRRLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHPPSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDLEAFQALYNCAQVLHALRASLRTVLMVVVYVHVDQNGYESFSPDPSEWEDVCRLDAGWGRTGTGRAATGDKGVNDASYQGANRSDGDDSDEEQDGEEDEANEGQCQSSPCPIFLPVGVPNLPRGAAIEFEVVALSTAFAQVKSPTRRAEQGRLEDILEEKDMGECPLPLPFQPPATFAALASKNMPLMSRPLLSYTWEAIFVPYVFNFVSLSLYLDDDLDGRNEGGQNETLLLNYTHVAYALVDQLRKILRVAHLTWQQLSHLRVFYIATCLDNEAMLREALAAALPSDGQCARTFLPVSSLMSECQEGSKRQDSLFLRVQVIALDLEKLRTEAWVHSVPDGGM
Sbjct:    1 MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKRRLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHPPSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDLEAFQALYNCAQVLHALRASLRTVLMVVVYVHVDQNGYESFSPDPSEWEDVCRLDAGWGRTGTGRAATGDKGVNDASYQGANRSDGDDSDEEQDGEEDEANEGQCQSSPCPIFLPVGVPNLPRGAAIEFEVVALSTAFAQVKSPTRRAEQGRLEDILEEKDMGECPLPLPFQPPATFAALASKNMPLMSRPLLSYTWEAIFVPYVFNFVSLSLYLDDDLDGRNEGGQNETLLLNYTHVAYALVDQLRKILRVAHLTWQQLSHLRVFYIATCLDNEAMLREALAAALPSDGQCARTFLPVSSLMSECQEGSKRQDSLFLRVQVIALDLEKLRTEAWVHSVPDGGM 847          

BLAST of EWM24946.1 vs. NCBI_GenBank
Match: gi|553180768|ref|XP_005853459.1| (hypothetical protein NGA_0485810 [Nannochloropsis gaditana CCMP526] >gi|422295603|gb|EKU22902.1| hypothetical protein NGA_0485810 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1596.64 bits (4133), Expect = 0.000e+0
Identity = 775/776 (99.87%), Postives = 776/776 (100.00%), Query Frame = 0

Query:   72 MSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKRRLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHPPSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDLEAFQALYNCAQVLHALRASLRTVLMVVVYVHVDQNGYESFSPDPSEWEDVCRLDAGWGRTGTGRAATGDKGVNDASYQGANRSDGDDSDEEQDGEEDEANEGQCQSSPCPIFLPVGVPNLPRGAAIEFEVVALSTAFAQVKSPTRRAEQGRLEDILEEKDMGECPLPLPFQPPATFAALASKNMPLMSRPLLSYTWEAIFVPYVFNFVSLSLYLDDDLDGRNEGGQNETLLLNYTHVAYALVDQLRKILRVAHLTWQQLSHLRVFYIATCLDNEAMLREALAAALPSDGQCARTFLPVSSLMSECQEGSKRQDSLFLRVQVIALDLEKLRTEAWVHSVPDGGM 847
            MSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKRRLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPR+HFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHPPSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDLEAFQALYNCAQVLHALRASLRTVLMVVVYVHVDQNGYESFSPDPSEWEDVCRLDAGWGRTGTGRAATGDKGVNDASYQGANRSDGDDSDEEQDGEEDEANEGQCQSSPCPIFLPVGVPNLPRGAAIEFEVVALSTAFAQVKSPTRRAEQGRLEDILEEKDMGECPLPLPFQPPATFAALASKNMPLMSRPLLSYTWEAIFVPYVFNFVSLSLYLDDDLDGRNEGGQNETLLLNYTHVAYALVDQLRKILRVAHLTWQQLSHLRVFYIATCLDNEAMLREALAAALPSDGQCARTFLPVSSLMSECQEGSKRQDSLFLRVQVIALDLEKLRTEAWVHSVPDGGM
Sbjct:    1 MSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKRRLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRVHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHPPSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDLEAFQALYNCAQVLHALRASLRTVLMVVVYVHVDQNGYESFSPDPSEWEDVCRLDAGWGRTGTGRAATGDKGVNDASYQGANRSDGDDSDEEQDGEEDEANEGQCQSSPCPIFLPVGVPNLPRGAAIEFEVVALSTAFAQVKSPTRRAEQGRLEDILEEKDMGECPLPLPFQPPATFAALASKNMPLMSRPLLSYTWEAIFVPYVFNFVSLSLYLDDDLDGRNEGGQNETLLLNYTHVAYALVDQLRKILRVAHLTWQQLSHLRVFYIATCLDNEAMLREALAAALPSDGQCARTFLPVSSLMSECQEGSKRQDSLFLRVQVIALDLEKLRTEAWVHSVPDGGM 776          

BLAST of EWM24946.1 vs. NCBI_GenBank
Match: gi|1173940551|gb|OQR89932.1| (hypothetical protein ACHHYP_05931 [Achlya hypogyna])

HSP 1 Score: 385.956 bits (990), Expect = 3.359e-119
Identity = 253/650 (38.92%), Postives = 355/650 (54.62%), Query Frame = 0

Query:    1 MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKRRLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHPPSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDLEAFQAL--YNCAQVLHALRASLRTVLMVVVYVHVDQNGYESFSPDPSEWEDVCRLDAGWGRTGTGRAATGDKGVNDASYQGANRSDGDDSDEEQDGEEDEANEGQCQSSPCPIFLPVGVPNLPRGAAIEFEVVALSTAFAQVKSPT 648
            M+ VALVSGGKDSV+S+ KC+ +GHE+V LA L PP D   E+DSFMFQS G+  V  +AEC+ +P+VR T++G S    + Y PTEGDEVEDL+ LL  V+  +PEV  V++GA+ S YQRTRVE+VC RLGL S+A+LW+ DQ +L++EM +  +  +LVKV+S GL P KHLG+SIA+L P F  L+ ++ FH+ GEGGEYETL +D P F++R+V+D   + +H+DD++ PVGL  I   H E KEES          P    + P + A      D  P   A   G        G RA  +R +           + +SG     P +               Q+      +Q +L      L +VVV+H+Y++ MA+FAA+NA Y    F   HPPSRSCV+L +A                V +D +AL G+G      +   R VLHV+SLS WAP+CIGPYSQA+ ++   +   AGQI L+PATM L+    L A  AL   N  +VL AL ++LR V   +VY     +  ES                    T     +   + + +A+   A+  + +   +E D E D+A      ++  P+ + V VP LP+ A +E EVVAL+    +   PT
Sbjct:    1 MKVVALVSGGKDSVYSLLKCVEHGHEIVCLATLTPPADN-AEVDSFMFQSIGTHVVASIAECMELPLVRRTLAGQSLATDMGYAPTEGDEVEDLYQLLVAVRAQFPEVTGVNTGAIFSNYQRTRVENVCARLGLTSIAYLWRKDQDELMQEMIDADMHCILVKVASMGLNPRKHLGRSIADLYPTFLDLHAKYQFHICGEGGEYETLTLDCPLFRKRIVVDAARIELHADDMFAPVGLYCIDALHLEAKEES----------PTAPPLPPAQTAATPPRLDFSPRRPALPFG--------GPRATCFRNQ-----------IALSGVACEDPSLPL-----------GDQVHAIFATLQELLARFDAGLSNVVVMHVYVQDMAAFAALNAVYGTY-FPGLHPPSRSCVQLDMA--------------AAVQIDCLALRGAGLNPRVPTPVQRSVLHVQSLSEWAPMCIGPYSQASTVAQS-VVLCAGQIPLEPATMALLPAPTLAANLALSFRNAGRVLEALDSNLRHVATTLVY-----HTLES--------------------TAAADVSVLGREILEANGHAADAFEHELDSDESDDEHDKAAARVALATRSPLAV-VAVPQLPKDAPVEVEVVALTHKALRAFPPT 567          

BLAST of EWM24946.1 vs. NCBI_GenBank
Match: gi|922479690|ref|XP_013637882.1| (PREDICTED: diphthine--ammonia ligase isoform X1 [Brassica oleracea var. oleracea])

HSP 1 Score: 361.688 bits (927), Expect = 9.843e-110
Identity = 223/539 (41.37%), Postives = 301/539 (55.84%), Query Frame = 0

Query:    1 MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKR-RLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHP---PSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDL-EAFQALYNCAQVLHALRASL-RTVLMVVVY 533
            M+ VALVSGGKDS + + KC+ YGHE+V LANL P  D  +ELDS+M+Q+ G   +   AEC+ VP+ R  + G+S HQ L Y  T  DEVEDL  LL  VK+  P + AVSSGA+ S YQR RVES+C RLGL SLAFLW+ DQ+ LL+EM   G++A+LVKV++ GL P KHLGK +A +EP   +L   +G +V GEGGEYETL +D P F   R+VLD+ +VV+HS D   PVG+LH    H E K+ +S  E   G              +V  +   GP+T   +      AV   +  R        H+ + G+    S  C        +G+  S+ G     +   L+  +  LL+ G +  +V+ +HLY+  M+ FAA N  Y +   H   P   PSRS +ELPL       EA  G   VEVL+               + Q +++LHV+S+S WAP CIGPYSQA L  H  +  +AGQ+GLDP TM L     + E  QAL N   +  A R S+  + L+ VV+
Sbjct:    1 MKVVALVSGGKDSCYVMMKCIQYGHEIVALANLLPVDDSVDELDSYMYQTVGHQIIVSYAECMNVPLFRRRIRGSSRHQKLSYQMTPDDEVEDLFVLLSEVKRQIPSITAVSSGAIASDYQRLRVESICSRLGLVSLAFLWKQDQTLLLQEMIANGIKAILVKVAAIGLDPSKHLGKDLAFMEPYLLKLKELYGSNVCGEGGEYETLTLDCPLFTNARIVLDEFQVVLHSPDSIAPVGVLHPTTFHLEKKDSNSLEEKEPG--------------LVFEVQGDGPNTPESTHQRDNRAVDVVEHTRN-----GVHLSKTGKENTFSICC------WLEGSSESSTG-LKEDLESVLKETESQLLKQGFNWQNVLYIHLYISDMSEFAAANETYVKFITHEKCPFGVPSRSTIELPLV------EAGLGKAYVEVLV--------------ANDQSKRILHVQSISSWAPSCIGPYSQATL--HKGVLHMAGQLGLDPPTMNLRNEGAVAELNQALTNSEAIAEAFRCSISSSALLFVVF 491          

BLAST of EWM24946.1 vs. NCBI_GenBank
Match: gi|1072965261|ref|XP_018432630.1| (PREDICTED: diphthine--ammonia ligase [Raphanus sativus])

HSP 1 Score: 358.992 bits (920), Expect = 1.260e-108
Identity = 222/538 (41.26%), Postives = 296/538 (55.02%), Query Frame = 0

Query:    1 MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKR-RLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHP---PSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDL-EAFQALYNCAQVLHALRASLRT--VLMVV 531
            M+ VALVSGGKDS + + KC+ YGHE+V LANL P  D  +ELDS+M+Q+ G   +   AEC+ VP+ R  + G+S HQ L Y  T  DEVEDL  LL  VK+  P + AVSSGA+ S YQR RVES+C RLGL SLAFLW+ DQ+ LL+EM   G++A+LVKV++ GL P KHLGK +A +EP   +L   +G +V GEGGEYETL +D P F   R+VLD+ +VV+HS D   PVG+LH    H E           KGN  +  L    E  +V  +   GP+T   +       V           R + H+ +  +    S  C        +G+  S+ G     +   L  ++  LL+ G    +V+ +HLY+  M+ FA  N  Y +   H   P   PSRS +ELPL       +A  G   VEVL+               + Q ++VLHV+S+S WAP CIGPYSQA L  H  +  +AGQ+GLDP TM L     + E  QAL N   +  A R S+ +  VL VV
Sbjct:    1 MKVVALVSGGKDSCYVMMKCIQYGHEIVALANLLPVDDSVDELDSYMYQTVGHQIIVSYAECMNVPLFRRRIRGSSRHQKLSYQMTPDDEVEDLFVLLSEVKRQIPSITAVSSGAIASDYQRLRVESICSRLGLVSLAFLWKQDQTLLLQEMIANGIKAILVKVAAIGLDPSKHLGKDLAFMEPYLLKLKELYGSNVCGEGGEYETLTLDCPLFTNARIVLDEFQVVLHSPDSIAPVGVLHPTIFHLE----------KKGNPDSDSLEEKKEPGLVFEVQGDGPNTPESTRQPDNGTVE--------HTRNRVHLSKTEKENTFSICC------WLEGSSESSTG-LKEDLETVLTEIESQLLKQGFKWQNVLYIHLYISDMSEFAVANETYVKFITHEKCPFGVPSRSTIELPLV------QAGLGKSYVEVLV--------------ANDQSKRVLHVQSISSWAPSCIGPYSQATL--HKGVLHMAGQLGLDPPTMNLRNEGAIAELNQALTNSEAIAEAFRCSISSSAVLFVV 491          

BLAST of EWM24946.1 vs. NCBI_GenBank
Match: gi|685383343|ref|XP_009123987.1| (PREDICTED: diphthine--ammonia ligase [Brassica rapa])

HSP 1 Score: 352.829 bits (904), Expect = 2.782e-106
Identity = 220/539 (40.82%), Postives = 296/539 (54.92%), Query Frame = 0

Query:    1 MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKR-RLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHP---PSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDL-EAFQALYNCAQVLHALRASL-RTVLMVVVY 533
            M+ VALVSGGKDS + + KC+ YGHE+V LANL P  D  +ELDS+M+Q+ G   +   AEC+ VP+ R  + G+S HQ L Y  T  DEVEDL  LL  VK+  P + AVSSGA+ S YQR RVES+C RLGL SLAFLW+ DQ+ LL+EM   G++A+LVKV++ GL P KHLGK +A +EP   +L   +G +V GEGGEYETL +D P F   R++LD+ +VV+HS D   PVG+LH    H E K+ +S  E   G              +V  +   GP+T   +       V           R +  V + G+    S  C        +G+  S+ G     +   L   +  LL+ G +  +V+ +HLY+  M+ FA  N  Y +   H   P   PSRS +ELPL       +A  G   VEVL+               + Q ++VLHV+S+S WAP CIGPYSQA L  H  +  +AGQ+GLDP TM L     + E  QAL N   +  A R S+  + L  VV+
Sbjct:    1 MKVVALVSGGKDSCYVMMKCIQYGHEIVALANLLPVDDSVDELDSYMYQTVGHQIIVSYAECMNVPLFRRRIRGSSRHQKLSYQMTPDDEVEDLFVLLSEVKRQIPSITAVSSGAIASDYQRLRVESICSRLGLVSLAFLWKQDQTLLLQEMIANGIKAILVKVAAIGLDPSKHLGKDLAFMEPHLLKLKELYGSNVCGEGGEYETLTLDCPLFTNARIMLDEFQVVLHSPDSIAPVGVLHPTVFHLEKKDSNSLEEKEPG--------------LVFEVQGDGPNTPESTCQPDNGTVE--------HTRNRVSVSKTGKENTFSICC------WVEGSSQSSTG-LREDLETVLTETESQLLKQGFNWQNVLYIHLYISDMSEFAVANETYVKFITHEKCPFGVPSRSTIELPLV------QAGLGKAYVEVLV--------------ANDQSKRVLHVQSISSWAPSCIGPYSQATL--HKGVLHMAGQLGLDPPTMNLRNEGAVAELNQALTNSEAIAEAFRCSISSSALHFVVF 488          

BLAST of EWM24946.1 vs. NCBI_GenBank
Match: gi|1072964028|ref|XP_018493053.1| (PREDICTED: diphthine--ammonia ligase-like isoform X1 [Raphanus sativus])

HSP 1 Score: 352.443 bits (903), Expect = 3.557e-106
Identity = 219/540 (40.56%), Postives = 300/540 (55.56%), Query Frame = 0

Query:    1 MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKR-RLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQ-MRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHP---PSRSCVELPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDL-EAFQALYNCAQVLHALRASL-RTVLMVVVY 533
            M+ VALVSGGKDS + + KC+ YGHE+V LANL P  D  +ELDS+M+Q+ G   +   AEC+ VP+ R  + G+S HQ L Y  T+GDEVED+  LL  VK+  P + AVSSGA+ S YQR RVES+C RLGL SLAFLW+ DQ+ LL+EM   G++A+LVKV++ GL P KHLGK +A +EP   +L   +G +V GEGGEYETL +D P F   R+VLD+ EV +HS D   PVG+LH    H E           KGN  +  +    E  +V  +   GP+T   +     C +  G        R + ++ + G+    S  C       +   DSS       + +   L  ++  +L+HGL+  +V+ +HLY+  M  FA  N  Y +       P   PSRS +ELPL       +A  G   VEVL+               +   ++VLHV+S+S WAP CIGPYSQA L  H  +  +AGQ+GLDP TM L     + E  QAL N   +  A R S+  + ++ VV+
Sbjct:    1 MKVVALVSGGKDSCYVMMKCIQYGHEIVALANLLPVDDSVDELDSYMYQTVGHQIIVSYAECMNVPLFRRRIRGSSRHQKLSYQMTQGDEVEDMFVLLSEVKRQIPSITAVSSGAIASDYQRLRVESICSRLGLVSLAFLWKQDQTLLLQEMIANGIKAILVKVAAIGLDPSKHLGKDLAFMEPHLLKLKELYGSNVCGEGGEYETLTLDCPLFTNARIVLDEFEVKLHSPDSIAPVGVLHPTIFHLE----------KKGNADSNSV--EEEPGLVFEVQGDGPNTPEST-----CQLDNGIVEHT---RTRVNLSKTGKENTFSICC-------WLEEDSSESSTGLKEDLETVLTEIETQILKHGLNWQNVLYIHLYISDMTEFAVANETYVKFITQEKCPFGVPSRSTIELPLV------QAGLGKAYVEVLV--------------ANDHSKRVLHVQSISSWAPSCIGPYSQATL--HKGVLHMAGQLGLDPPTMNLRNEGAIAELNQALTNSEAIAEAFRCSITSSAILFVVF 491          

BLAST of EWM24946.1 vs. NCBI_GenBank
Match: gi|1024933365|ref|XP_016311915.1| (PREDICTED: diphthine--ammonia ligase [Sinocyclocheilus anshuiensis])

HSP 1 Score: 349.747 bits (896), Expect = 1.202e-105
Identity = 220/550 (40.00%), Postives = 306/550 (55.64%), Query Frame = 0

Query:    1 MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKRRLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEP---NRC--------LVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHPPSRSCVE--LPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVK-PRDLEAFQALYNCAQVLHAL--RASLRTVLMVVVYV 534
            MR  AL+SGGKDS +++ +C++ GH +V LANL P    P+ELDS+M+Q+ G  AV   AE + VP+ R T+ G S H   +Y+ TEGDEVEDL+ LL  VK+    VEAVS GA+LS YQR RVE+VC RL LQ LA+LW+ DQ++LL+E+   GL+A+L+KV++ GL P +HLGKS+AE+E     L+ ++G H+ GEGGEYET  +D P FK+++V+D  E VIHS D + PVG L   R H E K E S      G  P   +RC        +   LE A       GG  ++AE    ++C    G            + W       ISG            A  S H +   Q R     +QR L E+GL +  V++VHLY+  M +F+ +N+ YC   +  H+PP+R C +  LP+++              ++ +DV+   G+    +   F  R  LHV+SLS WAP  IGPYSQA  +      F AGQI L P +M LVK P  L+A     +  +VL A+  R +L  VL   +Y+
Sbjct:    1 MRVAALISGGKDSCYNMLQCVSAGHSIVALANLMPHTHVPDELDSYMYQTVGHQAVGLYAEAMDVPLYRRTIEGFSLHTAREYSQTEGDEVEDLYQLLKLVKE-KEHVEAVSVGAILSDYQRVRVENVCARLQLQPLAYLWRRDQAELLREIISSGLQALLIKVAAYGLDPERHLGKSLAEMEAYLHELSEKYGVHICGEGGEYETFTVDCPLFKKKIVIDSMETVIHSADAFAPVGYLRFTRMHTEDKREGS------GGRPLPLSRCPCQNAIDSMTEELEYADKTPDVQGGCPSNAE----RSCQSELGCLPSHSSRSSSGYQW-------ISGL----------TALQSEHPDIQSQTRHVFTLLQRQLQENGLLMKHVLLVHLYVSDMENFSQINSVYC--TYFTHNPPARVCAQVWLPVSQ--------------QLRMDVLLQDGT-VVCEEDDFPQRDTLHVQSLSHWAPANIGPYSQAVQVREA--VFCAGQIALVPCSMQLVKGPVALQARLCFSHMEKVLCAMDSRLTLAHVLQAHIYI 503          

BLAST of EWM24946.1 vs. NCBI_GenBank
Match: gi|1042374144|ref|XP_017331419.1| (PREDICTED: diphthine--ammonia ligase [Ictalurus punctatus])

HSP 1 Score: 342.428 bits (877), Expect = 1.091e-102
Identity = 236/649 (36.36%), Postives = 326/649 (50.23%), Query Frame = 0

Query:    1 MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKRRLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEPNRCLVLPLEVAIVRSLADGGPSTSA---------ESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHPPSRSCVE--LPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVKPRDLEAFQALY-NCAQVLHALRASLRTVLMVVVYVHVDQNGYESFSPDPSEWEDVCRLDAGWGRTGTGRAATGDKGVNDASYQGANRSDGDDSDEEQDGEEDEANEGQCQSSPCPIFLPVGVPNLPRGAAIEFEVVAL 637
            M+ VAL+SGGKDS +++ +C+A GH +V LANL P     +ELDS+M+Q+ G  A+E  AE + +P+ R T+ G+S   G +Y+P + DEVEDL+ LL  VK+    VEAVS GA+LS YQR RVE+VC RL LQ LA+LW+ +Q+ LL EM   GL+A ++KV++ GL P  HLGK++AE+EP   +L++++G H+ GEGGEYETL ID P FK+R+V+D  E ++HSDD + PV  L   + H E K   S TE++          LPL     +S+ D     +          E   S AC      R+         + W  G    ISG+      I  Q   + T              +Q  L E G  +  +V+VHLY+R M  F  VNA Y    F  H PP+R CV+  LP+ +               + +DV+ L     A + G FQ R  LHV+S+S WAP  IGPYSQA  +      F AGQI L P TM L++       Q  Y +  +VL A+ ASL                                          G        V D ++  A R      D+ QD  E E   G  +   CP  +   +P+LPRGAA+E  V+A+
Sbjct:    3 MKVVALISGGKDSCYNMMQCVAAGHTIVALANLRPA--HTDELDSYMYQTVGHQAIELYAEAMDLPLYRRTIEGSSVDTGREYSPNDADEVEDLYQLLKLVKE-KEGVEAVSVGAILSDYQRVRVENVCSRLQLQPLAYLWRREQADLLSEMISSGLQAFVIKVAAYGLDPGTHLGKTLAEMEPYLHQLSKKYGVHICGEGGEYETLTIDCPLFKKRIVIDAMETIMHSDDAFAPVAYLRFTQMHTEDKTTGS-TESA----------LPLTSCPCQSVVDMMTEETVYADKAQDVQEEFASNACQFMSSARSSA------GYQWITG----ISGHSSEVTDIQSQAQHAFT-------------VLQGELQEKGWQMKHIVLVHLYVRDMEDFGKVNAVY--RIFFSHCPPARVCVQARLPVGQ--------------RLQMDVL-LHDWSVAPEVGCFQHRDTLHVQSISHWAPANIGPYSQAVQVDEA--VFCAGQIALVPCTMQLLRAGPRPQAQLCYSHMEKVLRAMSASLT----------------------------------------LGHTLQAHCYVTDHTHIPAVRKVWH--DKIQDSHEQEVCCG-VEEIQCPPLVVSVMPSLPRGAAVELHVIAV 552          

BLAST of EWM24946.1 vs. NCBI_GenBank
Match: gi|1025327792|ref|XP_016388558.1| (PREDICTED: diphthine--ammonia ligase-like [Sinocyclocheilus rhinocerous])

HSP 1 Score: 342.813 bits (878), Expect = 1.282e-102
Identity = 236/673 (35.07%), Postives = 341/673 (50.67%), Query Frame = 0

Query:    1 MRFVALVSGGKDSVFSIAKCLAYGHELVVLANLFPPVDGPEELDSFMFQSAGSSAVECLAECIGVPMVRATMSGASTHQGLQYTPTEGDEVEDLHALLFRVKQLYPEVEAVSSGAVLSTYQRTRVESVCDRLGLQSLAFLWQYDQSQLLKEMTEYGLEAVLVKVSSQGLAPHKHLGKSIAELEPLFKRLNRQHGFHVAGEGGEYETLAIDAPFFKRRLVLDDTEVVIHSDDVYTPVGLLHIHRCHAEFKEESSPTETSKGNEP---NRC--------LVLPLEVAIVRSLADGGPSTSAESLGSQACAVAPGKRARKWRIRPQPHVWRQGRVLNISGYCVPTPRIHFQGADSSTHGEAAGQMRLCLEGVQRVLLEHGLSLHDVVVVHLYLRSMASFAAVNAEYCRAPFHPHHPPSRSCVE--LPLARPYDDEEACRGWEEVEVLLDVMALAGSGAAMDAGSFQPRQVLHVKSLSGWAPVCIGPYSQANLLSHGHLAFLAGQIGLDPATMVLVK-PRDLEAFQALYNCAQVLHALRASLRTVLMVVVYVHVDQNGYESFSPDPSEWEDVCRLDAGWGRTGTGRAATGDKGVNDASYQGANRSDGDDSDEEQDGEEDEANEGQCQSSPCPIFLPVGVPNLPRGAAIEFEVVALSTAFAQVKSPTRRAEQGRLEDI 659
            MR  AL+SGGKDS +++ +C++ GH +V LANL P    P+ELDS+M+Q+ G  AV+  AE + VP+ R T+ G+S H G +Y+ TEGDEVEDL+ LL  VK+    VEAVS GA+LS YQR RVE+VC RL LQ LA+LW+ DQ++LL+E+   GL+A+L+KV++ GL P +HLGKS+AE+E     L+ ++G H+ GEGGEYET  +D P FK+++V+D  E VIHS D +    ++ ++              T  G  P   +RC        +   LE A       GG  ++AE    ++C   PG            + W       ISG            A  S H +   Q R     +QR L E GL +  +++VHLY+  M +F+ +N+ YC   +  H+PP+R CV+  LP+++              ++ +DV+   G+    +  +F  R  LHV+SLS WAP  IGPYSQA  +      F AGQI L P +M LVK P  L+A     +  +VL A+ +  R  L  V+  H+    +E  +     W                                        + + QD + + A   Q +SS         VP+LPRGAA+E  V A+      +  PT RA    +  I
Sbjct:    1 MRVAALISGGKDSCYNMLQCVSAGHSIVALANLMPHTHVPDELDSYMYQTVGHQAVDLYAEAMDVPLYRRTIEGSSLHTGREYSQTEGDEVEDLYQLLKLVKE-KEHVEAVSVGAILSDYQRVRVENVCARLQLQPLAYLWRRDQAELLREIISSGLQALLIKVAAYGLDPERHLGKSLAEMEAYLHELSEKYGVHICGEGGEYETFTVDCPLFKKKIVIDSMETVIHSADAFDTYRIIVLY--------------TGSGGRPLPLSRCPCQNAIDSMTEELEYADKTPDVQGGCPSNAE----RSCQSEPGCLPSHSSRSSSGYQW-------ISGL----------TALQSEHPDVQSQTRHVFTLLQRQLQEKGLLMKHMLLVHLYVSDMENFSQINSVYC--TYFTHNPPARVCVQVWLPVSQ--------------QLRMDVLLHDGTVVCKE-DAFPQRDTLHVQSLSHWAPANIGPYSQAVQVREA--VFCAGQIALVPCSMQLVKGPVALQARLCFSHLEKVLCAMDS--RLTLAHVLQAHIYITHHEHAAAVTEAW----------------------------------------NTKLQDVQGECAGAAQVESS------VAVVPSLPRGAAVELHVTAV------LDRPTDRASHLSVSQI 564