EWM25133.1, cds5569 (CDS) Nannochloropsis gaditana

BLAST of EWM25133.1 vs. NCBI_GenBank
Match: gi|585106771|gb|EWM25133.1| (Uncharacterized conserved protein UCP033563 [Nannochloropsis gaditana])

HSP 1 Score: 914.064 bits (2361), Expect = 0.000e+0
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0

Query:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYDDS 444
            MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYDDS
Sbjct:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYDDS 444          

BLAST of EWM25133.1 vs. NCBI_GenBank
Match: gi|553196252|ref|XP_005856301.1| (repb family protein, partial [Nannochloropsis gaditana CCMP526] >gi|422292779|gb|EKU20081.1| repb family protein, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 913.679 bits (2360), Expect = 0.000e+0
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0

Query:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYDDS 444
            MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYDDS
Sbjct:   17 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYDDS 460          

BLAST of EWM25133.1 vs. NCBI_GenBank
Match: gi|298712084|emb|CBJ26664.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 519.235 bits (1336), Expect = 2.812e-180
Identity = 258/447 (57.72%), Postives = 328/447 (73.38%), Query Frame = 0

Query:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLAD-GKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTG----ERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYD 442
            MV+V PF+  RPP  +A+ ++APPYDVI+SDEAR L     E SFLRVNKPE+DLP  ++ YD++VYERG +NLK+F+  GWL  +  P +Y+Y+Q+MG+H+Q GI     VE+YA GVIKRHEKTQVKKENDRTRLT+ QNAN GPVFL Y+  + +DEIV  +T   P E  F A +DG EH VW V D   V +I+RAFES++ +YIADGHHRSASAFRVGEMKIKEA+A G  VTG+E F  FLAVLFP+NQL  +EYNR+V+DL G ++++FL +V  NFEVS +                  P G+K V P    E+ MCLRG+WY ++A++ + P  DPV SLDVQ++Y+++L  VLNIG+PR DER+KYVGG+RG  EL+RLVT     E +W AVAF M+PVSV++VM +ADA+ LMPPKATWFEPKLRSGL+VR  D
Sbjct:    1 MVRVKPFRGLRPPPEMAAALSAPPYDVINSDEARELMKTAGETSFLRVNKPEVDLPPEINQYDVQVYERGNQNLKKFLENGWLVPDKTPCFYVYAQKMGEHLQTGIVAGVPVEDYALGVIKRHEKTQVKKENDRTRLTHVQNANAGPVFLTYRTSETVDEIVGRMTRENP-EVQFRA-EDGTEHSVWAVRDPVDVFAIQRAFESVKTTYIADGHHRSASAFRVGEMKIKEAVARGDVVTGEEPFNFFLAVLFPSNQLLCMEYNRVVRDLAGFSDEDFLERVSVNFEVSTT------------------PEGSK-VEPGRVREMAMCLRGSWYTIRAKDGTFPAEDPVKSLDVQILYDNLLNPVLNIGNPRADERIKYVGGIRGSNELQRLVTNPVPEEGSW-AVAFCMFPVSVDQVMRVADADQLMPPKATWFEPKLRSGLVVRSLD 425          

BLAST of EWM25133.1 vs. NCBI_GenBank
Match: gi|1082114727|gb|OFV82627.1| (hypothetical protein A2Y78_07155 [Acidobacteria bacterium RBG_13_68_16])

HSP 1 Score: 412.535 bits (1059), Expect = 1.187e-138
Identity = 227/442 (51.36%), Postives = 284/442 (64.25%), Query Frame = 0

Query:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYD 442
            M  V PF+A RP   LA  VAAPPYDVI+S+EAR +ADG E SFL +NKPEIDLP  +S YD RVY  GA+NL+ F+  G   +EA P +Y+Y QRMGDH+Q GI  A S  EYA G+IKRHE T+  KE+DRTR T+  NAN GPVFL Y+    +D IV  V    P    FVA  DGI H VW+V  E T  ++R AF  +   Y+ADGHHR+ASA RVG     E  AA     GDE +  FLAVLFP +QL+IL+YNR+VKDL GL+E +FL KV E F V+ +               EPRP  ++          GM L G WYRL+AR  S    DPV SLD  ++  ++L  VL I D R D+R+ +VGG+RGL ELE+   G+  W AVAFA++P S++++M +ADA  +MPPK+TWFEPKLRSGL+VR  D
Sbjct:    1 MSDVRPFRALRPRPELAERVAAPPYDVINSEEARSIADGNEISFLHINKPEIDLPPDVSLYDDRVYATGARNLRRFMGAGVFVREAEPRFYVYQQRMGDHVQAGIVCAASCREYADGLIKRHEFTRKDKEDDRTRHTHELNANAGPVFLTYRKRADVDAIVDGVRKASPV-YDFVA-SDGIAHTVWIVPAEQT-ETVRAAFGRVPALYVADGHHRAASAARVG----LERKAANPAHHGDEPYNYFLAVLFPHDQLRILDYNRVVKDLGGLSEKKFLEKVREKFTVAPA--------------PEPRPDASR--------RFGMLLGGKWYRLEARPGSFAADDPVRSLDAAILQENLLAPVLGIADVRTDKRIDFVGGIRGLGELEK--KGKEGW-AVAFALFPTSLDQLMSVADAGLVMPPKSTWFEPKLRSGLLVRTLD 410          

BLAST of EWM25133.1 vs. NCBI_GenBank
Match: gi|493987368|ref|WP_006930160.1| (hypothetical protein [Caldithrix abyssi] >gi|371721036|gb|EHO42807.1| Uncharacterized conserved protein UCP033563 [Caldithrix abyssi DSM 13497] >gi|1108620476|gb|APF18833.1| Uncharacterized conserved protein, DUF1015 family [Caldithrix abyssi DSM 13497])

HSP 1 Score: 407.142 bits (1045), Expect = 1.860e-136
Identity = 210/443 (47.40%), Postives = 277/443 (62.53%), Query Frame = 0

Query:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLV-TGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYD 442
            M  + PFK  RP   LA  VA+PPYDV+SS+EAR +A     SFL +NKPEIDLP   + YD  VY++G +NL  FI  G L ++  P +Y+Y Q MGDH Q G+    SVEEY   +IK+HE T+  KE DR +   T NA  GPVFL YK    +D ++  +    P E  F A DDGI H  WLV+ +  + +++  F+ +   Y+ADGHHRSA+A RV +M+     A   N TGDE +  FL V+FP NQ+ I++YNR+VKDLNGL+ D FL KV E F+V K G    KP +                    K E  M L G WYRLKAR  +  ++DPV  LDV ++ N++L  +L IGDPR+D+R+ +VGG+RGLEEL+R V +GE     VAF +YP S+E++M IADA  +MPPK+TWFEPKLRSGL++ L D
Sbjct:    1 MAIIKPFKGLRPKKELAEKVASPPYDVLSSEEAREMAKDNPFSFLHINKPEIDLPPETNIYDEAVYQKGKENLDRFIKEGILVQDEKPMFYVYRQIMGDHSQIGLVAGASVEEYEKNLIKKHELTRADKEEDRVKHVNTLNAQTGPVFLTYKARPEIDALMNKIVQGEP-EYDFTA-DDGIRHTFWLVDKDEDIKALQELFKQLDCLYVADGHHRSAAAMRVKQMR----QAQNPNHTGDEEYNYFLTVIFPHNQMYIMDYNRVVKDLNGLSVDAFLKKVSEKFDVQKLGASAEKPRQ--------------------KHEFVMYLNGEWYRLKARPGTFNENDPVEQLDVSILMNNLLVPILGIGDPRKDKRIDFVGGIRGLEELKRRVDSGEM---QVAFGLYPTSIEDLMAIADAGKIMPPKSTWFEPKLRSGLVIHLLD 414          

BLAST of EWM25133.1 vs. NCBI_GenBank
Match: gi|1176154676|gb|OQX71843.1| (hypothetical protein B6D62_01280 [Candidatus Cloacimonas sp. 4484_275])

HSP 1 Score: 399.438 bits (1025), Expect = 2.038e-133
Identity = 214/443 (48.31%), Postives = 279/443 (62.98%), Query Frame = 0

Query:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLV-TGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYD 442
            M K+ PF+  RP   L   VA+PPYDV+SS+EAR++A     SFL V KPEIDLP +++ YD  VY++G +NL + IS G L ++  P +Y Y   MG+  Q G+    S+E+Y   VIK+HE T+  KE DR +   T NAN GPVFL Y     +DE+   +T  +P        +DGI H VW V DE  +  I   F  I+  Y+ADGHHRSASA  VG+ +     AA  N TG+E +  FL+V+FP NQL I++YNR+VKDLNGL +DEFL KV E F+V     EEV               GNK  +P      GM L G WY+L A E + PK DPV SLDV ++  ++L  +L IGDPR+D+R+ +VGG+RGL+ELER V +GE    AVAF+M+P S+EE+M IADA  +MPPK+TWFEPKLRSG+IV L D
Sbjct:    1 MAKIRPFRGVRPKQELVEKVASPPYDVLSSEEARKMAAKNPFSFLHVVKPEIDLPENINIYDEAVYQKGKENLYKMISDGILIQDEKPCFYFYRLIMGNVNQIGLVAGASIEDYENDVIKKHEHTRADKEADRIKHVDTLNANTGPVFLTYPAQKEIDELTEKITGEKPLYD--FETEDGIRHTVWKVEDENRIKEIISLFSKIKNLYVADGHHRSASATIVGQRR----RAANPNHTGEEEYNFFLSVIFPHNQLYIMDYNRVVKDLNGLEKDEFLKKVGEKFKV-----EEVT--------------GNKPYKPEKAHTFGMYLDGKWYKLTALEGTYPKDDPVKSLDVSILQENLLAPILGIGDPRKDKRIDFVGGIRGLKELERRVDSGE----AVAFSMFPTSIEELMTIADAGKVMPPKSTWFEPKLRSGVIVHLLD 414          

BLAST of EWM25133.1 vs. NCBI_GenBank
Match: gi|491177028|ref|WP_005035394.1| (DUF1015 domain-containing protein [Holophaga foetida])

HSP 1 Score: 394.045 bits (1011), Expect = 2.263e-131
Identity = 208/443 (46.95%), Postives = 280/443 (63.21%), Query Frame = 0

Query:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYDD 443
            M ++ PF+AYRP   LA+ VA+ PYDVI++ EAR+L+ G   SFLRV +PE+DLP  +  +   VY +GA+NL  FI+ G L  E  P  Y+Y Q+  DH+Q G+   CSV+EY  G IK+HE T+  KE+DRTR    QNAN  PVFL Y+    +D IV  V  T P      A  DGI H VW ++DE  + ++   F+ I   YIADGHHR+A+A R  +   K  I AG   TG E F  F+AV+FP +QL+I++YNRIVKDLNGLT + FL+KV + F VS++     KP+       +PR               GM L G WYRL+A+  S P  DP+  LDV ++ +++L  +L I DPR D R+ +VGG+RG+ ELER V       AVAF++YP S+E++M +ADA  +MPPK+TWFEPKLRSGL++RLY+D
Sbjct:    1 MSQIKPFRAYRPKMGLAAKVASVPYDVINTQEARQLSAGNPFSFLRVGRPEVDLPEGVDEHSDAVYAKGAENLHRFITEGTLIHEDKPCLYVYQQKADDHVQTGLVALCSVKEYEDGFIKKHELTRKDKEDDRTRHVTEQNANAEPVFLTYRSVPYIDGIVDKVRDTAPLYDIVTA--DGIGHTVWCISDETVIYALSHLFDGIDNLYIADGHHRTAAAIRYSQA--KRLITAG--ATGAEPFESFMAVVFPHDQLRIMDYNRIVKDLNGLTPEAFLAKVAQVFNVSET--RRSKPE-------DPR-------------TFGMYLGGKWYRLEAKVGSFPATDPIRGLDVSILQDNLLGPILGITDPRTDSRIDFVGGIRGMGELERRVN---EGAAVAFSLYPTSIEQLMSVADAGQIMPPKSTWFEPKLRSGLLLRLYED 412          

BLAST of EWM25133.1 vs. NCBI_GenBank
Match: gi|740208521|ref|WP_038050430.1| (DUF1015 domain-containing protein [Thermoanaerobaculum aquaticum] >gi|632136085|gb|KDA52944.1| hypothetical protein EG19_08535 [Thermoanaerobaculum aquaticum])

HSP 1 Score: 390.578 bits (1002), Expect = 6.597e-130
Identity = 218/442 (49.32%), Postives = 289/442 (65.38%), Query Frame = 0

Query:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYD 442
            M  V PF+A RP   LA+ VAAPPYDV+SS+EAR+LA+GK  SFL +NKPEIDLP  +  YD RVY +GA NL++F++ G   +E  P +Y+Y Q+MG+H+Q G+  A SV EY  G+IKRHE T+  KE+DRTR     NAN  PVFL Y+   A+D +V  V +  P    FVA +DGI H VW+V  E   +++++AF+ +   Y+ADGHHR+A+A RVG    +E  A      GDE +  F+AVLFP NQL+IL+YNR+VKDL GL+ + FLSKV E F V K           A +G           RP  + + GM L+G WYRL+A   S  + DP+ SLDV ++  ++L  VL I DPR D+R+ ++GG+RGL  LE+ V     W AVAFA+YP S+E+VM +ADA  +MPPK+TWFEPKLRSGL+VR  D
Sbjct:    1 MSDVRPFRALRPRPELAAQVAAPPYDVVSSEEARKLAEGKPYSFLHINKPEIDLPPDVDLYDDRVYAQGASNLRKFMAEGVFFREQEPRFYVYQQKMGNHVQAGVVAAVSVAEYDAGLIKRHELTRKDKEDDRTRHVDELNANDEPVFLTYRQLAAIDALVDQVRAGAPL-YDFVA-EDGIGHTVWVVPSE-LAANLQQAFQQVPALYVADGHHRTAAAARVG----RERKAKNPQHRGDEPYNYFMAVLFPHNQLQILDYNRVVKDLGGLSPEAFLSKVAEKFRVEK-----------ASSG-----------RPEKRHQFGMYLQGQWYRLEAVPGSFREDDPIGSLDVSILQENLLAPVLGITDPRTDKRIDFIGGIRGLSALEQRVA--EGW-AVAFALYPTSLEQVMAVADAGLIMPPKSTWFEPKLRSGLLVRTLD 410          

BLAST of EWM25133.1 vs. NCBI_GenBank
Match: gi|1085181534|gb|OGT69797.1| (hypothetical protein A3H44_00050 [Gammaproteobacteria bacterium RIFCSPLOWO2_02_FULL_57_10])

HSP 1 Score: 389.423 bits (999), Expect = 1.960e-129
Identity = 206/443 (46.50%), Postives = 291/443 (65.69%), Query Frame = 0

Query:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLV-TGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYD 442
            M  + PF A RP   LA+ VAAPPYDV++ +EA  +A G E SFLR+NKPEID+  ++ +YD+RVY+RGA+NL++FI +G ++++A   +Y+Y Q MG H Q G+  A SVE Y   +IK+HE T+  KE+DR     T +A VGPVFL Y+    +D ++ TVT+T P E  FV+ DDG  H++W+V D+ TV  I+ AF+ +   Y+ADGHHRSA+A RV +++ K    A    TG ES+  F  V+FP NQ++IL+YNR+VKDL G++E+EFL  +   F V +S         GA             V+PT + E  M L G WY L+A + +  ++DPV SLDV ++ N++L  +L I DPR D+R+ +VGG+RGL+ LE+ V +GE  W A AFA+YP S++ +M IAD   +MPPK+TWFEPKL+SGL V + D
Sbjct:    1 MKLIRPFNALRPRKELAAKVAAPPYDVLNREEAFEMAKGNEYSFLRINKPEIDVDFAIDAYDMRVYQRGAENLQKFIDKGIMQRDARNTFYVYKQVMGKHEQVGLVAAASVEAYEKELIKKHEFTRPDKEDDRVNHINTLSAQVGPVFLTYRAQPEIDALIATVTATTP-EFDFVS-DDGNRHVLWVVADDATVQKIQAAFDKVPCLYVADGHHRSAAAQRVKDLRKK----ANPAHTGQESYNFFSTVIFPDNQMQILDYNRVVKDLKGMSEEEFLRAIQAWFNVHESKA-------GA-------------VKPTREHEFTMYLGGKWYILEAIDGTWIENDPVRSLDVSILQNNLLSPLLGIEDPRRDKRIDFVGGIRGLQALEKRVDSGE--WKA-AFALYPTSIKSLMAIADVGEVMPPKSTWFEPKLKSGLFVHILD 414          

BLAST of EWM25133.1 vs. NCBI_GenBank
Match: gi|652457369|ref|WP_026852209.1| (hypothetical protein [Geothrix fermentans])

HSP 1 Score: 385.571 bits (989), Expect = 6.429e-128
Identity = 203/442 (45.93%), Postives = 271/442 (61.31%), Query Frame = 0

Query:    1 MVKVLPFKAYRPPAALASTVAAPPYDVISSDEARRLADGKEDSFLRVNKPEIDLPASLSSYDLRVYERGAKNLKEFISRGWLRKEAAPAYYIYSQRMGDHIQHGICGACSVEEYATGVIKRHEKTQVKKENDRTRLTYTQNANVGPVFLMYKPHDALDEIVRTVTSTRPAETSFVADDDGIEHLVWLVNDEPTVSSIRRAFESIRFSYIADGHHRSASAFRVGEMKIKEAIAAGKNVTGDESFCLFLAVLFPANQLKILEYNRIVKDLNGLTEDEFLSKVMENFEVSKSGGEEVKPDEGAGNGGEPRPGGNKIVRPTVKGEVGMCLRGTWYRLKAREASVPKHDPVNSLDVQVVYNHVLKAVLNIGDPREDERMKYVGGVRGLEELERLVTGERAWGAVAFAMYPVSVEEVMDIADAEALMPPKATWFEPKLRSGLIVRLYD 442
            M ++ PF+AYRP   LA+ VAA PYDV+++ EA  LA G   SFL V +PEIDLPA    +   VY +G  NL   I  G L +E  P+ Y+Y QRMGDH+Q G+ G CSV+EY  G IKRHE T+  KE+DRTR    QNAN  PVFL YK    +D IV  +    P     V   DGI H VW+V  EP +  +   F  +   YIADGHHR+A+A R G+ +   A AA      DE F  F+AV+FP +QLKI++YNR+VKDLNGL+ D FL+KV + F+V+                    P  ++   P      GM L G WY LKA+  + P  DPV  LDV ++  ++L  +L I DPR + R+ +VGG+RG++ELER V   +   AVAF+++P S++++M +ADA  +MPPK+TWFEPKLRSGL+VR+Y+
Sbjct:    1 MSQLKPFRAYRPRPELAAQVAAVPYDVVNTQEAAELAKGNPHSFLHVGRPEIDLPAGTDVHADEVYAKGVANLMRMIETGTLMQEGLPSLYVYQQRMGDHVQAGLVGLCSVQEYENGTIKRHEFTRKDKEDDRTRHVTEQNANAEPVFLTYKAVPYIDHIVNDIRKLPPVYD--VVTPDGIGHTVWVVTGEPVICELVNLFNGVPALYIADGHHRTAAAIRYGQARRTAAGAAATG---DEPFESFMAVVFPHDQLKIMDYNRVVKDLNGLSPDAFLAKVRDKFDVA--------------------PAAHR--SPKAPTTFGMYLGGAWYELKAKPGTYPASDPVRGLDVSILQENLLGPILGIDDPRTNTRIDFVGGIRGMDELERRV---KNGYAVAFSLFPTSLDQLMSVADAGEVMPPKSTWFEPKLRSGLLVRMYE 412