EWM25182.1, cds5491 (CDS) Nannochloropsis gaditana

Overview
NameEWM25182.1
Unique Namecds5491
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002466.1:140187..141305 -

Link to JBrowse

Properties
Property NameValue
Protein idEWM25182.1
Productriboflavin kinase
Orig transcript idgnl|cribi|Naga_100527g2.3199.mrna
GbkeyCDS
Noteencoded by transcript variant 1; alternatively spliced
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002466.1supercontigCM002466.1:140187..141305 -
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Molecular Function
TermDefinition
GO:0016301kinase activity
GO:0008168methyltransferase activity
GO:0008531riboflavin kinase activity
Vocabulary: Biological Process
TermDefinition
GO:0009058biosynthetic process
GO:0008152metabolic process
GO:0009231riboflavin biosynthetic process
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR023468Riboflavin_kinase
IPR029063SAM-dependent_MTases
IPR023465Riboflavin_kinase_domain
IPR013216Methyltransf_11
IPR015865Riboflavin_kinase_bac/euk
Homology
BLAST of EWM25182.1 vs. NCBI_GenBank
Match: gi|585106842|gb|EWM25182.1| (riboflavin kinase [Nannochloropsis gaditana] >gi|585106843|gb|EWM25183.1| riboflavin kinase [Nannochloropsis gaditana])

HSP 1 Score: 748.043 bits (1930), Expect = 0.000e+0
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 0
Query:    1 MDLSAGMLVVARERFPRASFWQGDVLDLPVIYEREGGKEEGLFTAVFFNACFGNVFDQARALREVGKLVKKGGRVVISHPLGADFVEELRRRSPSVVLADLPRDEKAWRVGKEGRERGRDGGRAGRRERRNRDDMWSRGRSSSFLSLLPTIPSFPFLPCSFFQDILVTTPFQLTSLQSTSSLYLALLCRRPDQPLPQALLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGRECKAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAKRLVEEEGEGTGEEERGNGASKGKGRWVPFEEWGPR 372
            MDLSAGMLVVARERFPRASFWQGDVLDLPVIYEREGGKEEGLFTAVFFNACFGNVFDQARALREVGKLVKKGGRVVISHPLGADFVEELRRRSPSVVLADLPRDEKAWRVGKEGRERGRDGGRAGRRERRNRDDMWSRGRSSSFLSLLPTIPSFPFLPCSFFQDILVTTPFQLTSLQSTSSLYLALLCRRPDQPLPQALLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGRECKAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAKRLVEEEGEGTGEEERGNGASKGKGRWVPFEEWGPR
Sbjct:    1 MDLSAGMLVVARERFPRASFWQGDVLDLPVIYEREGGKEEGLFTAVFFNACFGNVFDQARALREVGKLVKKGGRVVISHPLGADFVEELRRRSPSVVLADLPRDEKAWRVGKEGRERGRDGGRAGRRERRNRDDMWSRGRSSSFLSLLPTIPSFPFLPCSFFQDILVTTPFQLTSLQSTSSLYLALLCRRPDQPLPQALLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGRECKAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAKRLVEEEGEGTGEEERGNGASKGKGRWVPFEEWGPR 372          
BLAST of EWM25182.1 vs. NCBI_GenBank
Match: gi|553187066|ref|XP_005854544.1| (riboflavin kinase [Nannochloropsis gaditana CCMP526] >gi|422294516|gb|EKU21816.1| riboflavin kinase [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 313/372 (84.14%), Postives = 314/372 (84.41%), Query Frame = 0
Query:    1 MDLSAGMLVVARERFPRASFWQGDVLDLPVIYEREGGKEEGLFTAVFFNACFGNVFDQARALREVGKLVKKGGRVVISHPLGADFVEELRRRSPSVVLADLPRDEKAWRVGKEGRERGRDGGRAGRRERRNRDDMWSRGRSSSFLSLLPTIPSFPFLPCSFFQDILVTTPFQLTSLQSTSSLYLALLCRRPDQPLPQALLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGRECKAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAKRLVEEEGEGTGEEERGNGASKGKGRWVPFEEWGPR 372
            MDLSAGMLVVARE FPRASFWQGDVLDLPVIYEREGGKEEGLFTAVFFNACFGNVFDQARALREVGKLVKKGGRVVISHPLGADFVEELRRRSPSVVLADLPRDEKAWR                                     +L T P                  FQLTSLQSTSSLYLALLCRRPDQPLPQALLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGRECKAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAKRLVEEEGEGTGEEERGNGASKGKGRWVPFEEWGPR
Sbjct:  208 MDLSAGMLVVARESFPRASFWQGDVLDLPVIYEREGGKEEGLFTAVFFNACFGNVFDQARALREVGKLVKKGGRVVISHPLGADFVEELRRRSPSVVLADLPRDEKAWR------------------------------------DILVTTP------------------FQLTSLQSTSSLYLALLCRRPDQPLPQALLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGRECKAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAKRLVEEEGEGTGEEERGNGASKGKGRWVPFEEWGPR 525          
BLAST of EWM25182.1 vs. NCBI_GenBank
Match: gi|299115578|emb|CBN75781.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 178.333 bits (451), Expect = 6.148e-48
Identity = 117/314 (37.26%), Postives = 151/314 (48.09%), Query Frame = 0
Query:   46 VFFNACFGNVFDQARALREVGKLVKKGGRVVISHPLGADFVEELRRRSPSVVLADLPRDEKAWRVGKEGRERGRDGGRAGRRERRNRDDMWSRGRSSSFLSLLPTIPSFPFLPCSFFQDILVTTPFQLTSLQSTSSLYLALLCRRPDQPLPQALLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGREC----------------------KAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAK 337
            V FNA FGN++DQ  AL     ++++GG+VVISHPLG DFV  L+    +VV  +LP  +   R+                                  +  LP +                     + S +S   LYLA+L + P +   +   L+G VS GY RGSKKLG PTANLP S F     +  +P GVY GWA + G                         K V N+GYSPTF G+EN EKIVE HLI G    DFYG  M ++L GF R E+KF SFP LVA I +DV DA +A+D P ++  K
Sbjct:  175 VVFNAVFGNLWDQGAALERAANILEEGGKVVISHPLGRDFVSRLKAVDDTVVPHELPERDALERL----------------------------------VQFLPLV---------------------IDSFESGPELYLAVLRKTPHRASGRVRYLRGTVSTGYGRGSKKLGVPTANLPESQFAEN--LRTLPTGVYFGWAALEGAANKEGEGGKGDASGGGGDGGEGLWKCVANVGYSPTFAGQENAEKIVEGHLI-GYEGEDFYGRTMRMLLAGFQRREKKFASFPELVATINKDVGDAAMALDEPRFSAFK 430          
BLAST of EWM25182.1 vs. NCBI_GenBank
Match: gi|1210513007|dbj|GAX28015.1| (riboflavin kinase [Fistulifera solaris])

HSP 1 Score: 168.703 bits (426), Expect = 2.276e-44
Identity = 124/333 (37.24%), Postives = 158/333 (47.45%), Query Frame = 0
Query:   42 LFTAVFFNACFGNVFDQARALREVGKLVKK------GGRVVISHPLGADFVEELRRRSPSVVLADLPRDEKAWRVGKEGRERGRDGGRAGRRERRNRDDMWSRGRSSSFLSLLPTIPSFPFLPCSF--FQDILVTTPFQLTSLQSTSS-LYLALLCRRPDQPLPQALLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGREC--KAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAKRLVEEEGEGTGEEERGNGASKGKGRW 363
            LF  +  NACFGN +     L    +L+K       GG + I+HPLGA+FV +L + +P  V   LP  E+   + +                                           FLP     FQ        Q  S+Q  +   Y A L R     LPQ +  +GPV+ GY RG KKLGFPTANL  ++F  +  +E +  GVY GWA + GR    KAVVN+G SPTF+G EN EKI+EAHL+      DFYGE M L L GFLRPE KF SF ALVA I  D ++A+ A+DL  Y   K   +   E       GN    G+  W
Sbjct:  182 LFDGIVMNACFGNFWSGLEVLERAAQLLKPPVPQSPGGFLFITHPLGAEFVAQLHQETPETVPHCLPNHEQLIHMTR-------------------------------------------FLPLQLIEFQ--------QRVSVQGEAQPFYCATLERVRHCCLPQLMRFRGPVASGYGRGGKKLGFPTANLEPAVF--RKALETVSTGVYFGWAVIEGRNACHKAVVNVGISPTFEGAENAEKIIEAHLLLEEPIDDFYGETMRLQLHGFLRPETKFDSFSALVAQIAADAKEAKEALDLRPYVTLKS--DTFLERNSPTWIGNSGGDGQASW 459          
BLAST of EWM25182.1 vs. NCBI_GenBank
Match: gi|1210511474|dbj|GAX29288.1| (riboflavin kinase [Fistulifera solaris])

HSP 1 Score: 163.696 bits (413), Expect = 2.009e-42
Identity = 94/177 (53.11%), Postives = 112/177 (63.28%), Query Frame = 0
Query:  170 PFQLTSLQSTSSL-------YLALLCRRPDQPLPQALLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGREC--KAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAK 337
            P QL  LQ + SL       Y   L R     LPQ +  +GPV+ GY RG KKLGFPTANL  +LF  +  +E +P GVY GWA + GR    KAVVN+G+SPTF+G EN EKIVEAHL+      DFYGE M L L GFLRPE KF SFPALVA I  D ++A+ A+DL  Y+  K
Sbjct:  260 PLQLIDLQKSVSLTGEAQPFYCGTLERVRHCCLPQLMRFRGPVASGYGRGGKKLGFPTANLEPALF--RKALETVPTGVYFGWAVIEGRNACHKAVVNVGFSPTFEGAENAEKIVEAHLLLEESIDDFYGETMRLQLHGFLRPEIKFDSFPALVAQITADAKEAKEALDLRPYSTLK 434          
BLAST of EWM25182.1 vs. NCBI_GenBank
Match: gi|551659236|ref|XP_005832387.1| (hypothetical protein GUITHDRAFT_71408 [Guillardia theta CCMP2712] >gi|428176523|gb|EKX45407.1| hypothetical protein GUITHDRAFT_71408 [Guillardia theta CCMP2712])

HSP 1 Score: 150.599 bits (379), Expect = 6.555e-41
Identity = 74/132 (56.06%), Postives = 97/132 (73.48%), Query Frame = 0
Query:  201 LQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVG---RECKAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMD 329
            ++G V+ G+ RG KKLG PTANLP S+F     + ++  GVY GWA++ G   +  KAVVN+GYSPTF G ENREK+VEAHL++  F  DFYG+ M L+LTGFLRPE KF SFP L+ AI +D+E++R A+D
Sbjct:    1 MRGDVASGWGRGGKKLGIPTANLPESMFA--DALREVQTGVYLGWAQIGGVSKKSVKAVVNVGYSPTFVGAENREKVVEAHLLE-KFENDFYGKEMRLMLTGFLRPETKFDSFPELLEAIHKDIENSREALD 129          
BLAST of EWM25182.1 vs. NCBI_GenBank
Match: gi|219118032|ref|XP_002179799.1| (predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1] >gi|217408852|gb|EEC48785.1| predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 147.517 bits (371), Expect = 1.027e-39
Identity = 80/148 (54.05%), Postives = 97/148 (65.54%), Query Frame = 0
Query:  199 LLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGREC---------KAVVNIGYSPTFQGEENREKIVEAHLIDGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAK 337
            L L+G V  GY RG KKLGFPTANLPS LF  ++ ++D+P GVY GWA +   E          KAVVN+G+SPTF+G+EN EKIVEAHL+      DFY E M L L GFLRPE KF SFP L+  I  DV DA+ A+D+  + G K
Sbjct:    1 LRLRGKVDSGYGRGGKKLGFPTANLPSRLF--QNALQDVPAGVYFGWALLESDELAAPGRNIAHKAVVNVGFSPTFEGQENAEKIVEAHLMAEEPLTDFYNETMRLQLHGFLRPEIKFSSFPDLIKQINADVVDAKEALDVSPFVGFK 146          
BLAST of EWM25182.1 vs. NCBI_GenBank
Match: gi|224001818|ref|XP_002290581.1| (predicted protein [Thalassiosira pseudonana CCMP1335] >gi|220974003|gb|EED92333.1| predicted protein [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 149.058 bits (375), Expect = 2.502e-38
Identity = 86/183 (46.99%), Postives = 111/183 (60.66%), Query Frame = 0
Query:  199 LLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARV--------VGREC--KAVVNIGYSPTFQGEENREKIVEAHLI---------------------DGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMD-LP-VYAGAKRLVEEEGEGTG 348
            L L+GPV+ GY RG K+LG PTANLP+SLF  +S +ED+  GVY GWA V        +GR    KAVVN+GYSPTF+G+EN+EKIVEAHLI                     D    GDFYGE M L L GFLR E+KF SFP L+A I +D+ +A  A+D +P +++     +++ G   G
Sbjct:  127 LRLRGPVATGYGRGGKQLGVPTANLPASLF--QSALEDVATGVYFGWAVVEAPSTDSTIGRNTPIKAVVNVGYSPTFEGKENKEKIVEAHLITSNSPMEKNELADEYSTSSADDTKIEGDFYGETMRLQLIGFLRAERKFDSFPDLIAQIHRDIGNAAWALDSMPYIFSKEAEFIQDSGHWVG 307          
BLAST of EWM25182.1 vs. NCBI_GenBank
Match: gi|551572851|ref|XP_005772244.1| (hypothetical protein EMIHUDRAFT_444832 [Emiliania huxleyi CCMP1516] >gi|551600167|ref|XP_005781851.1| riboflavin kinase [Emiliania huxleyi CCMP1516] >gi|485623660|gb|EOD19815.1| hypothetical protein EMIHUDRAFT_444832 [Emiliania huxleyi CCMP1516] >gi|485634647|gb|EOD29422.1| riboflavin kinase [Emiliania huxleyi CCMP1516])

HSP 1 Score: 137.887 bits (346), Expect = 1.958e-35
Identity = 81/151 (53.64%), Postives = 95/151 (62.91%), Query Frame = 0
Query:  190 RPDQPLPQALLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARVVGRECKAVVNIGYSPTFQGEENREKIVEAHLI--DGAFPGDFYGELMTLILTGFLRPEQK--FPSFPA-LVAAIQQDVEDARVAMDLPVYAG 335
            R  QPLP    L GPV QGY RGS KLGFPTANLP SLF  +  +  +  GVY GWA + G   K V NIG SP+F GEEN E IVEAH+I  D   P DFYGE + L+L GF+RPE+K  F + P  L+AAI  DV  A   +D P +A 
Sbjct:   31 RAVQPLPALPALSGPVLQGYGRGSAKLGFPTANLPCSLF--QDRLVQLERGVYLGWAALGGDVRKCVANIGISPSFVGEENAETIVEAHVIAEDAPLPADFYGETLRLLLLGFVRPERKFDFTNGPGELIAAIGADVATATAELDRPPFAA 179          
BLAST of EWM25182.1 vs. NCBI_GenBank
Match: gi|1072233803|gb|OEU18533.1| (riboflavin kinase, partial [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 136.346 bits (342), Expect = 2.842e-35
Identity = 78/165 (47.27%), Postives = 99/165 (60.00%), Query Frame = 0
Query:  199 LLLQGPVSQGYKRGSKKLGFPTANLPSSLFVVKSLIEDIPVGVYCGWARV------VGRECKAVVNIGYSPTFQGEENREKIVEAHLI--------------------DGAFPGDFYGELMTLILTGFLRPEQKFPSFPALVAAIQQDVEDARVAMDLPVYAGAK 337
            L  +G V +GY RG KKLG PTANLPSSLF  ++ +ED+  GVY GWA +      + +  KAVVN+GYSPTF+G+EN+EKI+EAHLI                          DFYG  M L L GFLR E+KF SFP L+A I  DV+DAR+++D   Y   +
Sbjct:    1 LRYRGSVDEGYGRGGKKLGVPTANLPSSLF--QNALEDVATGVYFGWAAIERNNNELLQVYKAVVNVGYSPTFEGKENKEKIIEAHLILEKEEEEEDHNENDGGDDDKTSNIIEDFYGIPMRLQLLGFLREEKKFDSFPELIAQILADVDDARLSLDCHPYQSCR 163          
The following BLAST results are available for this feature:
BLAST of EWM25182.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Position :
0
Zoom :
x 1
01SequenceEWM25182.1XP_005854544.1CBN75781.1GAX28015.1GAX29288.1XP_005832387.1XP_002179799.1XP_002290581.1XP_005772244.1OEU18533.1
Match NameE-valueIdentityDescription
gi|585106842|gb|EWM25182.1|0.000e+0100.00riboflavin kinase [Nannochloropsis gaditana] >gi|5... [more]
gi|553187066|ref|XP_005854544.1|0.000e+084.14riboflavin kinase [Nannochloropsis gaditana CCMP52... [more]
gi|299115578|emb|CBN75781.1|6.148e-4837.26conserved unknown protein [Ectocarpus siliculosus][more]
gi|1210513007|dbj|GAX28015.1|2.276e-4437.24riboflavin kinase [Fistulifera solaris][more]
gi|1210511474|dbj|GAX29288.1|2.009e-4253.11riboflavin kinase [Fistulifera solaris][more]
gi|551659236|ref|XP_005832387.1|6.555e-4156.06hypothetical protein GUITHDRAFT_71408 [Guillardia ... [more]
gi|219118032|ref|XP_002179799.1|1.027e-3954.05predicted protein, partial [Phaeodactylum tricornu... [more]
gi|224001818|ref|XP_002290581.1|2.502e-3846.99predicted protein [Thalassiosira pseudonana CCMP13... [more]
gi|551572851|ref|XP_005772244.1|1.958e-3553.64hypothetical protein EMIHUDRAFT_444832 [Emiliania ... [more]
gi|1072233803|gb|OEU18533.1|2.842e-3547.27riboflavin kinase, partial [Fragilariopsis cylindr... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna5505rna5505Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications