EWM25445.1, cds5214 (CDS) Nannochloropsis gaditana

BLAST of EWM25445.1 vs. NCBI_GenBank
Match: gi|585107206|gb|EWM25445.1| (rna family [Nannochloropsis gaditana])

HSP 1 Score: 1162.13 bits (3005), Expect = 0.000e+0
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0

Query:    1 MCRPTRQDKLHPRASKMAFVLLLIRNTIGRRVASDSFSPSAGFSCQSPVSTFVRAGSCIGSYLPARYSLGRGGSTIRYLTEDEEGGMILSKGTRSVVTGSKQRRGRKSKPWQGPFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVEPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPRGGEIREIGFLKLGSRALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVFVEAHRTGAGAGPVPPWAGEGGTASL 566
            MCRPTRQDKLHPRASKMAFVLLLIRNTIGRRVASDSFSPSAGFSCQSPVSTFVRAGSCIGSYLPARYSLGRGGSTIRYLTEDEEGGMILSKGTRSVVTGSKQRRGRKSKPWQGPFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVEPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPRGGEIREIGFLKLGSRALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVFVEAHRTGAGAGPVPPWAGEGGTASL
Sbjct:    1 MCRPTRQDKLHPRASKMAFVLLLIRNTIGRRVASDSFSPSAGFSCQSPVSTFVRAGSCIGSYLPARYSLGRGGSTIRYLTEDEEGGMILSKGTRSVVTGSKQRRGRKSKPWQGPFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVEPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPRGGEIREIGFLKLGSRALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVFVEAHRTGAGAGPVPPWAGEGGTASL 566          

BLAST of EWM25445.1 vs. NCBI_GenBank
Match: gi|298715093|emb|CBJ27781.1| (RNA methyltransferase, TrmA family [Ectocarpus siliculosus])

HSP 1 Score: 462.996 bits (1190), Expect = 5.748e-155
Identity = 236/450 (52.44%), Postives = 297/450 (66.00%), Query Frame = 0

Query:  114 PFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVEPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPRGGEIREIGFLK-----------LGS---RALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRK--PKGATLLLRE-TREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVFVEAHR 546
            P+ Y EEL + +ESLTNLG GI R                          E   GWVVMVP VIPGE ++ +++RNH NYSEA L+ V E SP+RVEP CPLFG CGGCQYQHMSV+ QR WK + V+  L R G +   AV++T GT+ T GYRSK+TPH++ P  G +REIGF K           LG+     +VDV  C +AT  IN  L   R+E R   +AA  +   ++   +GATLL RE T EG VTD  A VS++VGG++F + AG FFQNNPYVLPHMV+YVV +A     + L+DAYCGGGLFCLSAS +FE C G+EIS +A+  A RNA  N I NC F AG A++IFA +   + ++TV I+DPPRKGCD+ FL QL +FRP R+VYVSCDPATQARDA+ ++  GY+  ++QPFDLFPQTRHIENVI F +  +
Sbjct:   92 PYEYLEELTVRIESLTNLGEGIAR--------------------------EGETGWVVMVPHVIPGELVKCKVFRNHANYSEATLVSVEEPSPDRVEPNCPLFGMCGGCQYQHMSVSMQRDWKNQQVMDCLTRIGGMVDPAVKDTFGTDETYGYRSKLTPHYDAPYQGRVREIGFKKVKFPTIYHGVDLGNVNPSKVVDVERCPLATEGINESLPPARQEARERVQAAFDSGDGKRVGKRGATLLFRETTEEGTVTDNNAHVSERVGGMVFNFKAGEFFQNNPYVLPHMVDYVVARAKEGGAKNLIDAYCGGGLFCLSASTSFEKCAGIEISPMAVDCATRNAATNDISNCLFLAGTASNIFANVS-FTPSETVVIVDPPRKGCDQLFLGQLFKFRPTRLVYVSCDPATQARDAKEIMANGYEAVDIQPFDLFPQTRHIENVITFQDNRK 514          

BLAST of EWM25445.1 vs. NCBI_GenBank
Match: gi|1200352242|gb|OUU71942.1| (SAM-dependent methyltransferase [Coraliomargarita sp. TMED73])

HSP 1 Score: 424.861 bits (1091), Expect = 1.583e-141
Identity = 224/437 (51.26%), Postives = 279/437 (63.84%), Query Frame = 0

Query:  114 PFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVEPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPRGGEIREIGFLKLGSR-ALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVFVEAHRTGA 549
            P+ YH ELEL V+SLTNLG GICRA+                           +GWVVMVP VIPGER+R RIYRNH NYSEADL+ VL+ SP+RV+PAC LF TCGGCQYQH+    Q   K   V++++ + G +    V+  +G+     YRSKITPH+E+P     + IGFL+ G R  +VDV  C IAT AIN  L G R E R +          R+ +G TLLLR   EGVVTDP+A VS++VGGL F++ AG FFQNNPY+LP MV+YV+ +A    V+YLVDAYCG GLF LSA+ +F    GVE+S+ A+L AQ N  +++I N  F  G A  IF G+  +   +T  I+DPPRKGCD  F  QL  +RPAR+VYVSC+PATQ RD    L+ GY IE +QPFDLFP TRHIENVI  V     G+
Sbjct:   13 PYPYHYELELIVDSLTNLGSGICRAT---------------------------DGWVVMVPFVIPGERVRARIYRNHSNYSEADLVEVLDRSPDRVDPACRLFQTCGGCQYQHIEYGRQLEEKTARVVELMEKAG-LGEHRVEPAVGSPQLYHYRSKITPHYERPARDGSQPIGFLQHGRRRTIVDVESCPIATQAINEVLPGARAEARESGGKK------RRQRGGTLLLRHVLEGVVTDPKAVVSERVGGLTFQFRAGEFFQNNPYILPQMVDYVIGEAKFGGVQYLVDAYCGSGLFALSAASSFTQVAGVEVSDQAVLWAQANCRISRIENARFLIGKAEAIFKGLSFLP-EETTVIIDPPRKGCDASFRDQLMGYRPARVVYVSCNPATQTRDVEEFLQSGYRIERIQPFDLFPHTRHIENVITLVGPEENGS 414          

BLAST of EWM25445.1 vs. NCBI_GenBank
Match: gi|1200287066|gb|OUU09256.1| (SAM-dependent methyltransferase [Opitutae bacterium TMED40])

HSP 1 Score: 419.468 bits (1077), Expect = 1.292e-139
Identity = 217/429 (50.58%), Postives = 272/429 (63.40%), Query Frame = 0

Query:  114 PFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVEPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPRGGEIREIGFLKLGSR-ALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVF 541
            P+ YH ELE+ +ES++NLG GI R                             +GWV+ +P V+PGE IR RIYRNHKNYS+AD + VLE SP RV+P+C LFG CGGCQYQ +  + Q +WK++HV +   R G +    V  T+ +     YRSK+TPH+EK    E R+IGFL+ GSR  LVDVP C IAT  INA L   RE +R         +T  K KG TLLLR+T EGV+T+P+  VS++VG L F++ AG FFQNNP++LP +V+YV+ +A +     LVDAYCGGGLF LSA+  F+   G+EIS      A+ NA LNKI N  F  GDA  IF+ +G         I+DPPRKGCD  F+ Q   FRP RIVYVSCDPATQARDA+ LL  GY I ++QPFDLFPQTRHIENV+  
Sbjct:   11 PYPYHHELEILIESVSNLGIGIARD----------------------------KGWVIQIPFVLPGETIRARIYRNHKNYSDADCLEVLEPSPHRVQPSCDLFGVCGGCQYQAVDYSTQLSWKQKHVEETFLRIGGIE-TTVNPTMASPKRFAYRSKLTPHYEKTLDTENRKIGFLRHGSRRILVDVPTCPIATDPINAALPEEREILR--------TQTMGKKKGGTLLLRDTIEGVITNPKETVSERVGDLTFQFKAGEFFQNNPFLLPQLVQYVIAEANSEVCPVLVDAYCGGGLFGLSAASQFDRVIGIEISREGFEGARTNALLNKIDNAEFFLGDAVSIFSELGQ-ENEPCALIIDPPRKGCDPDFIDQTLAFRPQRIVYVSCDPATQARDAKLLLNGGYRISQIQPFDLFPQTRHIENVLTM 401          

BLAST of EWM25445.1 vs. NCBI_GenBank
Match: gi|502809244|ref|WP_013044220.1| (class I SAM-dependent RNA methyltransferase [Coraliomargarita akajimensis] >gi|293615343|gb|ADE55498.1| RNA methyltransferase, TrmA family [Coraliomargarita akajimensis DSM 45221])

HSP 1 Score: 417.927 bits (1073), Expect = 5.768e-139
Identity = 214/427 (50.12%), Postives = 273/427 (63.93%), Query Frame = 0

Query:  114 PFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVEPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPRGGEIREIGFLKLGSR-ALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVI 539
            PF YH+ELELTV+SLTNLG G+CR                              GWVVMVP VIPGER+R+R+YRN+ NYS+ADL+ VLE S +RV P CPL+ TCGGCQYQH+    Q A K  HV +++ + G +    ++  IG+     YRSKITPH+++      + IGFL+ G R  +VDV +C IAT  IN  L   REE R++          R+ +G TLLLR   EGVVTDP+A VS++V  + F++ AG FFQNNP++LP MV+YV  +A A   RYLVDAYCG GLF LS +K+FE   GVE+S+ A+L AQ N  ++ I N  F  G A  IF G+      +T  I+DPPRKGCDE F  QL +FRP R+VYVSCDPATQARD +  ++ GY +  +QPFDLFP TRHIENV+
Sbjct:   13 PFEYHQELELTVDSLTNLGMGVCRDG---------------------------NGWVVMVPFVIPGERVRVRVYRNYSNYSDADLLEVLEPSADRVTPQCPLYQTCGGCQYQHVDYERQLAEKTGHVKELMEKIGELDH-PIEGAIGSPEVYYYRSKITPHYQRQNKDGSQPIGFLQFGRRNQIVDVEQCPIATREINEALPEAREEARLSGGKK------RRQRGGTLLLRHVLEGVVTDPKAVVSERVRDVTFQFKAGEFFQNNPFILPKMVDYVEAEASADGARYLVDAYCGSGLFALSTAKSFEQVAGVEVSDQAVLWAQANCRISGISNARFLIGKAEAIFNGL-KFPADETALIIDPPRKGCDESFRLQLLQFRPQRLVYVSCDPATQARDVKAFVDGGYRVTRIQPFDLFPHTRHIENVV 404          

BLAST of EWM25445.1 vs. NCBI_GenBank
Match: gi|1200339365|gb|OUU59632.1| (SAM-dependent methyltransferase [Opitutae bacterium TMED60])

HSP 1 Score: 416.772 bits (1070), Expect = 1.408e-138
Identity = 216/429 (50.35%), Postives = 274/429 (63.87%), Query Frame = 0

Query:  114 PFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVEPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPRGGEIREIGFLKLGSR-ALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVF 541
            P+ YH E++L +E+LTNLG+GI R                             +GWV+ VP V+PGE IR RIYRNHKNYS+AD + +L+SSP+RV+  C LFGTCGGCQYQ +    Q  WKR  V +   + G +T +    T  +  T GYRSK+TPH+EK R G  R++GFLK GSR  LVDVP C IAT +IN        E    AR A   ET  K KG TLLLR+ +EGV TDP+  VS++V G++F++ AG FFQNNP++LP +V++V+ +A   N   L+DAYCGGGLF LSAS+ F+   G+EIS      A+ NA LN+I N  F  GDA+ IF  + ++    ++ I+DPPRKGCD  FL Q   FRP RIVYVSCDPATQARDA+ L   GY I + QPFDLFPQTRHIENV+  
Sbjct:   13 PYPYHHEVDLKIETLTNLGFGIARD----------------------------DGWVIQVPFVLPGELIRARIYRNHKNYSDADSIEILQSSPDRVDAKCKLFGTCGGCQYQSVDYQKQLEWKRNQVNECFEKIGGLT-ITTMPTFPSPKTYGYRSKLTPHYEKSREGMERKLGFLKHGSRRILVDVPSCPIATTSIN--------EFLPQARTALFKET--KKKGGTLLLRDVQEGVATDPKEVVSERVNGIVFQFKAGEFFQNNPFILPDLVDHVIEQANPGNSISLIDAYCGGGLFSLSASRRFQKVVGIEISREGFEGARTNALLNQIKNAEFFLGDASSIFQELQNLPRPCSL-IVDPPRKGCDADFLRQCIEFRPNRIVYVSCDPATQARDAKILTXGGYRILQAQPFDLFPQTRHIENVLTL 401          

BLAST of EWM25445.1 vs. NCBI_GenBank
Match: gi|1154905571|ref|WP_078811420.1| (SAM-dependent methyltransferase [Prosthecobacter debontii] >gi|1152229958|emb|SKA76482.1| 23S rRNA (uracil1939-C5)-methyltransferase [Prosthecobacter debontii])

HSP 1 Score: 408.297 bits (1048), Expect = 3.038e-135
Identity = 206/429 (48.02%), Postives = 264/429 (61.54%), Query Frame = 0

Query:  114 PFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVEPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPRGGEIREIGFLKLGSR-ALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVF 541
            PF YH+EL++ +E+LTN G G+ R                             +GWVV VP  +PGE++R R+YRNHKNYS ADL+ VLE S +RV+P C LFGTCGGCQYQH+  A Q  WKR  V ++L     +    V+  I +    GYRSKITPH  KP+GGEI  IGFL+ G+R A+VDV  C IA PA+N  L  +R + R    A           GATLL+R    GV+T       +QVG + F++ AG FFQNNP++LP  V Y V +A A   R+LVDAYCG GLF +SA+++FE   GVE+SE A+  A  NAE+N + NC F A DA +IF  + H +G +TV I+DPPR GC E FL QL  F P R+VY+SC+PATQ RD     E GY + +VQPFDLFPQT+H+E V+  
Sbjct:   11 PFTYHQELDVRIENLTNEGSGVARV----------------------------DGWVVFVPFALPGEKVRCRVYRNHKNYSNADLVEVLEPSADRVQPQCTLFGTCGGCQYQHLDYARQTEWKRRQVEELLKHMAKIEH-PVEPVIASPQQYGYRSKITPHFHKPKGGEIGAIGFLRAGTRSAMVDVEHCPIAMPALNEELAAVRAQARANQDAFK--------NGATLLMRAAVNGVLTKADQIAIEQVGDVKFEFQAGDFFQNNPFILPEFVGYAVAEARASGARFLVDAYCGSGLFGISAARDFEEVLGVELSESAVRKAAHNAEINGLTNCKFLAADAREIFKEVPH-AGQETVVIIDPPRAGCSEEFLQQLFAFDPKRVVYISCNPATQMRDLNLFTEAGYKLGKVQPFDLFPQTKHLECVMTL 401          

BLAST of EWM25445.1 vs. NCBI_GenBank
Match: gi|494602814|ref|WP_007361066.1| (class I SAM-dependent RNA methyltransferase [Opitutaceae bacterium TAV1] >gi|391219673|gb|EIP98093.1| SAM-dependent methyltransferase, tRNA(uracil-5)-methyltransferase [Opitutaceae bacterium TAV1])

HSP 1 Score: 408.297 bits (1048), Expect = 6.662e-135
Identity = 225/450 (50.00%), Postives = 274/450 (60.89%), Query Frame = 0

Query:  105 GRKSKPWQGPFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERV-EPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPR------GGEIREIGFLKLGSR-ALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVFVEAHR 546
            GRK K    PFAYH E+EL + +LTNLG G+ R  L               P+  GG ++    WVVMVP  +PGER+R R++RNHKNYSEADL+ VL  SPERV  P CP+FG CGGCQYQ++   AQ  WKR  V ++L     +    V   IG+    GYRSKITPH   PR            +GFL+ G+R  ++DVP C IAT AINARL     EVR   RA   A   ++  GATLLLR+    V T     V++ VG L  ++ A  FFQNNP++LP    YV  +A A   R+LVDAYCG GLF L+A+  FE   G+EISE +I  A++NAELN+I N  FQAGDA+ IFAG+     ADT  I+DPPRKGCDE FL QL  + P  +VYVSCDPATQ RD R  L  GY +  VQPFDLFPQTRH+E VI      R
Sbjct:    5 GRKKKLNDQPFAYHAEIELEIATLTNLGVGLGRVPL--------------APAADGGEQK----WVVMVPFALPGERVRARVFRNHKNYSEADLLDVLTPSPERVATPPCPVFGRCGGCQYQNLDYDAQLRWKRRQVAELLRHMAGIEA-EVSSVIGSPRQYGYRSKITPHFNLPRDRGDPAAAATLPVGFLRQGTRFDILDVPRCEIATEAINARL----PEVRAATRAKVAAGDYQR--GATLLLRDASGAVTTRYDDIVTETVGDLRLRFLARDFFQNNPFILPAFTGYVREQAAASGARFLVDAYCGSGLFALTAASAFERVAGIEISESSIAFARQNAELNRIKNATFQAGDASAIFAGLA-FPAADTAVIIDPPRKGCDEPFLQQLFGYGPRAVVYVSCDPATQMRDLRHFLAAGYALTAVQPFDLFPQTRHLECVITLRRGRR 428          

BLAST of EWM25445.1 vs. NCBI_GenBank
Match: gi|1002801581|gb|KXU36065.1| (SAM-dependent methyltransferase [Cephaloticoccus capnophilus])

HSP 1 Score: 409.068 bits (1050), Expect = 8.152e-135
Identity = 227/453 (50.11%), Postives = 274/453 (60.49%), Query Frame = 0

Query:  114 PFAYHEELELTVESLTNLGWGICRASLPS------------------VPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVEPACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPRGGEIREIGFLKLGSR-ALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGG------LLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVF 541
            PFAYH E+EL + +LTN G G+ R  LP                    PD + P    PT STP   + S   WVVMVP  +PGER+R+R++RNHKNYSEADL+ VL +SP RVEP C LFG CGGCQYQH+  A Q  WKR  V ++L     +    V   IG+    GYRSKITPH +  R      IGFL+ G+R  ++DVPECAIATP INARL  +RE  R         E     + ATLLLR   EGVVTD  A +++++        L  ++ A  FFQNNP++LP    YV  +A +   R+LVDAYCG GLF LSA+  FE   GVEISE +I  AQ NA  N I N  F AGDA  IFAG+      +T  I+DPPR+GCDEGFL QL  F P  +VYVSCDPATQ RD R  LE GY ++ VQPFDLFPQTRH+E VI  
Sbjct:   13 PFAYHAEIELEISTLTNRGHGLGRVELPEEGAAPREGLDAGRADNAGTPDKEGPPDLEPTRSTPS--KNSLRQWVVMVPFCLPGERVRVRVFRNHKNYSEADLLKVLRASPHRVEPYCALFGQCGGCQYQHLDYAEQLVWKRRQVEELLQHMVGIEH-EVAPVIGSPRQYGYRSKITPHFKVGRPA----IGFLRQGTRFEVIDVPECAIATPEINARLVEVRERTRQR------LERREYKRDATLLLRHASEGVVTDYDAVITEELARGDAQEPLKLRFLARDFFQNNPFILPAFTGYVREQAASSGGRFLVDAYCGSGLFALSAASAFEQAAGVEISESSIRFAQENAVANGITNARFLAGDAAAIFAGL-DFPPEETAVIIDPPRRGCDEGFLGQLFAFGPRAVVYVSCDPATQMRDLRLFLEAGYALKAVQPFDLFPQTRHLECVITL 451          

BLAST of EWM25445.1 vs. NCBI_GenBank
Match: gi|1034530666|ref|WP_064500617.1| (class I SAM-dependent RNA methyltransferase [Opitutaceae bacterium TAV3] >gi|1033662207|gb|OAS32378.1| SAM-dependent methyltransferase [Opitutaceae bacterium TAV3])

HSP 1 Score: 408.683 bits (1049), Expect = 1.137e-134
Identity = 226/449 (50.33%), Postives = 279/449 (62.14%), Query Frame = 0

Query:  105 GRKSKPWQGPFAYHEELELTVESLTNLGWGICRASLPSVPDAQSPEMPLPTPSTPGGPRESPEGWVVMVPGVIPGERIRLRIYRNHKNYSEADLMHVLESSPERVE-PACPLFGTCGGCQYQHMSVAAQRAWKREHVLQVLARTGNVTGVAVQETIGTEHTLGYRSKITPHHEKPR---GGEIRE--IGFLKLGSR-ALVDVPECAIATPAINARLRGLREEVRVTARAAAVAETPRKPKGATLLLRETREGVVTDPRAEVSDQVGGLLFKYTAGSFFQNNPYVLPHMVEYVVRKAGAPNVRYLVDAYCGGGLFCLSASKNFEACYGVEISELAILSAQRNAELNKIGNCHFQAGDATDIFAGIGHISGADTVTILDPPRKGCDEGFLTQLCRFRPARIVYVSCDPATQARDARFLLERGYDIEEVQPFDLFPQTRHIENVIVFVEAHR 546
             +K +    PF YH E+EL + +LTNLG G+ R S+P+            +    GG +     WVVMVP  +PGER+R R++RNHKNYSEADL+ VL  SP+RV  P CPLFG CGGCQYQ+ + AAQ  WKR  V ++L     +    V   IG+    GYRSKITPH    R   G  + +  IGFL+ G+R  L+DVP C IAT AINARL  +R EV+  A A A        +GATLLLR+    V T+    V++QVG L  ++ A  FFQNNP++LP    YV  +A A   R+LVDAYCG GLF LSA+  FE   G+EISE +I  A++NA LN I N  FQAGDA+ IFAG+     ADT  I+DPPRKGCDE FL QL  + P  +VYVSCDPATQ RD R  L  GY++  VQPFDLFPQTRH+E VI    A R
Sbjct:    2 AKKKRFNDQPFPYHAEIELEIATLTNLGVGLGRVSIPAA-----------STEGEGGGQTGDSQWVVMVPFALPGERVRARVFRNHKNYSEADLLAVLTPSPDRVTTPPCPLFGRCGGCQYQNFNYAAQLVWKRRQVGELLRHMAGIES-PVSPVIGSPRQYGYRSKITPHFNPRRDASGAIVADMPIGFLRQGTRFDLLDVPRCEIATDAINARLTEVRAEVQTKAAAGAYV------RGATLLLRDASGVVTTNYDDIVTEQVGDLRLRFLARDFFQNNPFILPAFTGYVREQAAASGARFLVDAYCGSGLFALSAASAFERVTGIEISESSIAFARQNAALNGITNTTFQAGDASAIFAGLT-FPAADTAVIIDPPRKGCDESFLQQLFGYGPRTVVYVSCDPATQMRDLRHFLAAGYELTAVQPFDLFPQTRHLECVITLRAASR 431