EWM25406.1, cds5211 (CDS) Nannochloropsis gaditana

BLAST of EWM25406.1 vs. NCBI_GenBank
Match: gi|585107167|gb|EWM25406.1| (dna topoisomerase 6 subunit a [Nannochloropsis gaditana])

HSP 1 Score: 1173.3 bits (3034), Expect = 0.000e+0
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query:    1 MVRRRSVKDDTSSEEEEEEEVSSSDEEDEDSDFSPWEATTRKGKKITKAAPEQSIARSHTAATLTSSASKKKSPSQPRSLGKKEGKRGGGHGTVPRKAAAARTGTTSPGTLAGRKRRKLEREEDDEEEGKEEDEAKEVYDEDEEEDDDDEPEGKSRGGSTRRMTGKNQRKTKGLNVTGVGNSKVDKVLEEVRQLMLRSRRDDAPNPTLADLGITTVREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573
            MVRRRSVKDDTSSEEEEEEEVSSSDEEDEDSDFSPWEATTRKGKKITKAAPEQSIARSHTAATLTSSASKKKSPSQPRSLGKKEGKRGGGHGTVPRKAAAARTGTTSPGTLAGRKRRKLEREEDDEEEGKEEDEAKEVYDEDEEEDDDDEPEGKSRGGSTRRMTGKNQRKTKGLNVTGVGNSKVDKVLEEVRQLMLRSRRDDAPNPTLADLGITTVREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI
Sbjct:    1 MVRRRSVKDDTSSEEEEEEEVSSSDEEDEDSDFSPWEATTRKGKKITKAAPEQSIARSHTAATLTSSASKKKSPSQPRSLGKKEGKRGGGHGTVPRKAAAARTGTTSPGTLAGRKRRKLEREEDDEEEGKEEDEAKEVYDEDEEEDDDDEPEGKSRGGSTRRMTGKNQRKTKGLNVTGVGNSKVDKVLEEVRQLMLRSRRDDAPNPTLADLGITTVREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573          

BLAST of EWM25406.1 vs. NCBI_GenBank
Match: gi|698777825|ref|XP_009821956.1| (hypothetical protein H257_00790 [Aphanomyces astaci] >gi|574124081|gb|ETV89556.1| hypothetical protein H257_00790 [Aphanomyces astaci])

HSP 1 Score: 574.704 bits (1480), Expect = 0.000e+0
Identity = 265/387 (68.48%), Postives = 325/387 (83.98%), Query Frame = 0

Query:  187 VLEEVRQLMLRSRRDDAPNPTLADLGITTVREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573
            VL++V QL  ++       PTLA+L +  VREV +M P  V  EIE  ++     ILRGEGF Y VP+R+  NQ+Y+ ELDRIVL+DK++ R F N   VRKTAITT++M+LVH++L+KGIH+TKRD+FYTDVKLF+DQ +SD +LDDVACM+GCTRS L+VVASEKG+VVGR+ F +DGD IDCTRMGVGGKAIPPY+D++  I SDAKFILLVEK+AA+MRLAEDRFYNKYPCI++TAKGQPDVATR+FLK+++ ELH+PVLGLVDSDPYGLKILSVYM+GSKNMSYDSASLT  DIKWLGVRPSDL KY IP QCRL M + D++TG+ +L+E+FI+KNP W KEL+LMLK K+KAEIQALS FGFQ++T++YLP+KL  GDWI
Sbjct:   56 VLKKVNQLT-QALASGGQLPTLAELNVFNVREVREMVPTEVSTEIESMVVGVVSSILRGEGFRYAVPSRTTANQIYVTELDRIVLADKLSVRDFTNMSAVRKTAITTKVMELVHQLLAKGIHVTKRDLFYTDVKLFKDQSESDTILDDVACMVGCTRSSLHVVASEKGVVVGRIAFRDDGDLIDCTRMGVGGKAIPPYIDRLTDITSDAKFILLVEKDAAFMRLAEDRFYNKYPCIILTAKGQPDVATRMFLKRIQNELHLPVLGLVDSDPYGLKILSVYMSGSKNMSYDSASLTCRDIKWLGVRPSDLTKYKIPDQCRLPMKETDIKTGKDMLEEEFIKKNPLWSKELELMLKSKEKAEIQALSTFGFQFLTQEYLPQKLRDGDWI 441          

BLAST of EWM25406.1 vs. NCBI_GenBank
Match: gi|971520589|dbj|GAQ77576.1| (Topoisomerase 6 subunit A homolog 3 [Klebsormidium nitens])

HSP 1 Score: 563.918 bits (1452), Expect = 0.000e+0
Identity = 263/390 (67.44%), Postives = 326/390 (83.59%), Query Frame = 0

Query:  185 DKVLEEVRQLMLRSRRDDAPNPTLADLGITTV-REVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573
            D++L +V +L    ++    + TL DL +  V +EV D+    V+ EIEKT+L+ A  IL G GF Y +PTR++ NQLY+ +LDRIVL DK + R + N   VRKTAITTRI+QL+H++ +KGIH+TKRD+FYTDVKLF++Q  SD +LDDV+CMLGCTRS LNVV SEKG+VVGR+ F EDGD IDCT+MGVGGKAIPP +DK+   QSDAKFILLVEK+AA+MRLAEDRFYN++PC++ITAKGQPDVATRLFLKK+K+EL IPVLGLVDSDPYGLKILSVYM+GSKNMSYDS++LTT DIKWLGVRPSDLD++ IP QCRL MTD DM+TG+++L+EDFI+K+P W  EL+LM+K K+KAEIQALS+FGFQY+TE YLP KL++GDWI
Sbjct:   28 DEILAKVAKLSEAEKKKPKESVTLQDLNLPNVSKEVVDLGAPQVISEIEKTMLSVAASILNGHGFGYTIPTRTSANQLYVPQLDRIVLKDKSSFRQYGNMSTVRKTAITTRILQLIHQLCNKGIHVTKRDLFYTDVKLFQEQGQSDTILDDVSCMLGCTRSSLNVVGSEKGVVVGRLIFVEDGDVIDCTKMGVGGKAIPPNIDKVTGFQSDAKFILLVEKDAAFMRLAEDRFYNRFPCVIITAKGQPDVATRLFLKKMKSELKIPVLGLVDSDPYGLKILSVYMSGSKNMSYDSSNLTTPDIKWLGVRPSDLDRFKIPEQCRLPMTDADMKTGKEMLEEDFIKKDPRWHAELELMVKKKEKAEIQALSSFGFQYLTEVYLPMKLQEGDWI 417          

BLAST of EWM25406.1 vs. NCBI_GenBank
Match: gi|219111201|ref|XP_002177352.1| (type II DNA topoisomerase VI subunit [Phaeodactylum tricornutum CCAP 1055/1] >gi|217411887|gb|EEC51815.1| type II DNA topoisomerase VI subunit [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 555.829 bits (1431), Expect = 0.000e+0
Identity = 256/358 (71.51%), Postives = 313/358 (87.43%), Query Frame = 0

Query:  216 VREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573
            + EV ++    VL  +E   L  A  +L  +GFS ++P+R+A+NQ+Y+ E DRIVL  K ++R+FLN +E RK+AIT R+MQL+H VL K IHITKRD+FYTDVKLF DQ +SD VLDDVA M+GCTRS L+VVAS+KGLVVGR+QFEEDGDFIDCT+MGVGGKAIPPY+DKI  I SDA+FILLVEKEAAYMR+AEDRFY++YPCI+ITAKGQPDVATR+FL ++ +EL IPVLGLVDSDPYGLKILSVYM+GSKNMSYDSASLTT DIKWLG+RPSDLDKY +P QCRLDMT++D++TG+++++EDFIQKNPEW+KEL++M+K KKKAEIQALS+FGFQY+TE YLPRKL++GDWI
Sbjct:   73 IVEVVELPATQVLEGMEAMALHIAQQVLERKGFSLEIPSRAASNQIYVKEWDRIVLGGKRSSRSFLNVKESRKSAITLRVMQLLHAVLMKRIHITKRDLFYTDVKLFVDQAESDGVLDDVATMIGCTRSNLHVVASDKGLVVGRIQFEEDGDFIDCTKMGVGGKAIPPYIDKIENIASDAEFILLVEKEAAYMRMAEDRFYHRYPCIIITAKGQPDVATRMFLARITSELKIPVLGLVDSDPYGLKILSVYMSGSKNMSYDSASLTTPDIKWLGLRPSDLDKYDLPEQCRLDMTENDIKTGKEMMKEDFIQKNPEWMKELEIMVKTKKKAEIQALSSFGFQYVTEQYLPRKLKEGDWI 430          

BLAST of EWM25406.1 vs. NCBI_GenBank
Match: gi|754345858|ref|XP_004363895.2| (topoisomerase [Capsaspora owczarzaki ATCC 30864] >gi|765551399|gb|KJE92020.1| topoisomerase [Capsaspora owczarzaki ATCC 30864])

HSP 1 Score: 555.444 bits (1430), Expect = 0.000e+0
Identity = 257/387 (66.41%), Postives = 318/387 (82.17%), Query Frame = 0

Query:  187 VLEEVRQLMLRSRRDDAPNPTLADLGITTVREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573
            VL+EV +L    ++      TL +LGI+  REV+ M    V   IE+ ++ AA  IL G GFSY VP+R+++NQLY+ +L+RIVL   V+ R F +   VRKTAITTR++ LVHEVL+K IHITKRD+FYTDVKLF  Q DSD VLDDVACM+GCTR+ LNVVASEKG+V+GRV F +DGDFIDCT+MGVGGKAIP  +D+I  +Q DA+F+LLVEK+AA+MRL+EDRFYN+YPCI+ITA+GQPDV+TRLFL  +KT L+IP+LGLVDSDPYGLKILSVY++GSKNMSYDSASLTTSDIKWLGVRPSDL+ Y IP +CRLDMT+ D++ G+ LL+EDFI+ NP W+ EL+LMLK K+KAEIQALS FGFQY+T+ YLP KL++GDWI
Sbjct:   33 VLQEVNKLAKAQQQSVKHRATLEELGISDAREVAVMDDADVAQTIERIVIQAAQSILAGTGFSYDVPSRASSNQLYVPQLERIVLKSAVSNRPFSSTATVRKTAITTRVLSLVHEVLNKRIHITKRDLFYTDVKLFEKQNDSDAVLDDVACMIGCTRTSLNVVASEKGVVIGRVTFRDDGDFIDCTKMGVGGKAIPSLIDRITDLQGDAQFVLLVEKDAAFMRLSEDRFYNQYPCIIITARGQPDVSTRLFLNMVKTRLNIPILGLVDSDPYGLKILSVYLSGSKNMSYDSASLTTSDIKWLGVRPSDLETYNIPKECRLDMTESDIKYGQDLLKEDFIKNNPAWMAELELMLKTKEKAEIQALSNFGFQYLTQTYLPLKLQQGDWI 419          

BLAST of EWM25406.1 vs. NCBI_GenBank
Match: gi|698576587|ref|XP_009776251.1| (PREDICTED: DNA topoisomerase 6 subunit A [Nicotiana sylvestris] >gi|1025122055|ref|XP_016463309.1| PREDICTED: DNA topoisomerase 6 subunit A-like [Nicotiana tabacum])

HSP 1 Score: 554.673 bits (1428), Expect = 0.000e+0
Identity = 257/388 (66.24%), Postives = 322/388 (82.99%), Query Frame = 0

Query:  187 VLEEVRQLMLRSRRDDAPNPTLADLGI-TTVREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573
            +L+ ++ L   S    +  PTLAD+G+ TT REV+D+  + V + I   +L  A  IL GEGFS+ VP+RS+ NQLY+ ELDRI+L DK + RAF N   VRKT ITTRI+QL+H++ +K IH+TKRD+FYTDVKLF+DQ  SD VLDDV+C+LGCTRS LNVVA+EKG+VVGR+ F +DGD IDCT+MG+GGKAIPP +D++  +QSDA FILLVEK+AAYMRLAEDRFYN++PCI++TAKGQPDVATRLFL+K+K EL++PVL LVDSDPYGLKILSVY  GSKNMSYDSA+LTT DIKWLG+RPSDLDKY IP QCRL MT+ D++TG+ LL+EDF++KNP WV+EL LM+K K+KAEIQALS+FGFQY++E YLP KL++ DW+
Sbjct:   33 ILQTLKNLKASSAASSSKTPTLADVGLSTTCREVTDLPIDEVQYNIGSVMLNLARSILAGEGFSFNVPSRSSANQLYVPELDRIILKDKSSIRAFSNVSTVRKTTITTRILQLIHQLCTKNIHVTKRDLFYTDVKLFQDQTQSDTVLDDVSCILGCTRSSLNVVAAEKGVVVGRLIFSDDGDMIDCTKMGMGGKAIPPNIDRVGDMQSDALFILLVEKDAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELNLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDIKTGKDLLEEDFVKKNPAWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQEQDWL 420          

BLAST of EWM25406.1 vs. NCBI_GenBank
Match: gi|168022302|ref|XP_001763679.1| (predicted protein [Physcomitrella patens] >gi|162685172|gb|EDQ71569.1| predicted protein [Physcomitrella patens])

HSP 1 Score: 553.903 bits (1426), Expect = 0.000e+0
Identity = 258/388 (66.49%), Postives = 323/388 (83.25%), Query Frame = 0

Query:  187 VLEEVRQLMLRSRRDDAPNPTLADLGI-TTVREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573
            +L+ V +    ++  D    TLADL +  + +EV ++    V+ E+EK+ILA A  IL G GF Y +P+RS+ NQLY+ ELDRIVL DK + R F N   VRKTAITTRI+QLVH++ +K IH+TKRD+FYTDVKLF++Q  SD +LDDV+CMLGCTRS LNVVASEKG+VVGR+ F EDGD IDCT+MGVGGKAIPP +DK+  ++SDA FILLVEK+AA+MRLAEDRFYN++PC+++TAKGQPDVATRLFLKK+KT+L +PVLGLVDSDPYGLKILSVYM+GSKNMSYDS++LTT DIKWLGVRPSDL+KY IP QCRL M+D D++TG+ +L+EDFI+K+  WVKEL+LM+K K+KAEIQALS+FGFQY++E YLP KL++ DWI
Sbjct:   26 ILKIVTKFAEAAKEADVETRTLADLNLANSCKEVVELNTRSVVLEVEKSILAIAKSILSGNGFGYLIPSRSSANQLYVPELDRIVLKDKASFRQFANIASVRKTAITTRILQLVHQLCTKQIHVTKRDLFYTDVKLFQEQGQSDTILDDVSCMLGCTRSSLNVVASEKGVVVGRLIFIEDGDRIDCTKMGVGGKAIPPNIDKVQGMESDALFILLVEKDAAFMRLAEDRFYNRFPCVILTAKGQPDVATRLFLKKMKTDLKLPVLGLVDSDPYGLKILSVYMSGSKNMSYDSSNLTTPDIKWLGVRPSDLNKYKIPEQCRLPMSDQDIKTGKDMLEEDFIKKDKNWVKELELMVKTKEKAEIQALSSFGFQYLSEVYLPLKLQQEDWI 413          

BLAST of EWM25406.1 vs. NCBI_GenBank
Match: gi|552825097|ref|XP_005846746.1| (hypothetical protein CHLNCDRAFT_31596 [Chlorella variabilis] >gi|307106398|gb|EFN54644.1| hypothetical protein CHLNCDRAFT_31596 [Chlorella variabilis])

HSP 1 Score: 553.518 bits (1425), Expect = 0.000e+0
Identity = 270/422 (63.98%), Postives = 326/422 (77.25%), Query Frame = 0

Query:  153 GKSRGGSTRRMTGKNQRKTKGLNVTGVGNSKVDKVLEEVRQLMLRSRRDDAPNPTLADLGIT-TVREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573
            GK + G+   +T  N++K K           VD++L +V+ L  R + +     TLA+L +    REV D     VL EIE  IL  A  +LRGEGF+Y +P+RS  NQLY+ ELDRIVL D  + RAF N    RK  ITTRI+QLVHE+ +K IH+TKRD+FYTDVKLF DQ +SD +LDD+ACMLGCTRS L+VVASEKG+VVGR+ F EDGD IDC RMGVGGKAIPP +DK+  I SDA FILLVEK+AA+MRLAEDRFYN YPCI+ITAKGQPDVATRLFL+KLK  L IPVL LVDSDPYGLKILSVYM GS NMSYDS++LTT DIKWLGVRPSDLD++ IP QCRL MTD D++TG++LL+EDF++ NPEWVKEL++M+  K KAEIQALS+FGFQY+++ YLP KL++GDWI
Sbjct:    7 GKKKAGT--ELTALNKKKLKS----------VDEILGKVKWLAERVKGEPEVQKTLAELNLPHACREVVDKDAGAVLDEIETAILFVAASVLRGEGFTYTLPSRSKANQLYVPELDRIVLKDSQSHRAFANTSTCRKAVITTRILQLVHELCTKRIHVTKRDLFYTDVKLFEDQSNSDAILDDLACMLGCTRSSLHVVASEKGVVVGRLTFREDGDPIDCRRMGVGGKAIPPNIDKVDAITSDAMFILLVEKDAAFMRLAEDRFYNTYPCIIITAKGQPDVATRLFLRKLKQVLRIPVLALVDSDPYGLKILSVYMKGSMNMSYDSSNLTTPDIKWLGVRPSDLDRFSIPQQCRLPMTDEDIKTGKKLLEEDFVKANPEWVKELEIMVTTKVKAEIQALSSFGFQYLSQQYLPLKLQEGDWI 416          

BLAST of EWM25406.1 vs. NCBI_GenBank
Match: gi|1111065262|ref|XP_019239263.1| (PREDICTED: DNA topoisomerase 6 subunit A [Nicotiana attenuata] >gi|1102270309|gb|OIT21152.1| dna topoisomerase 6 subunit a [Nicotiana attenuata])

HSP 1 Score: 551.592 bits (1420), Expect = 0.000e+0
Identity = 256/388 (65.98%), Postives = 322/388 (82.99%), Query Frame = 0

Query:  187 VLEEVRQLMLRSRRDDAPNPTLADLGI-TTVREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573
            +L+ ++ L + S    +   TLAD+G+ TT REV+D+  + V + I   +L  A  IL GEGFS+ VP+RS+ NQLY+ ELDRI+L DK + RAF N   VRKT ITTRI+QL+H++ +K IH+TKRD+FYTDVKLF+DQ  SD VLDDV+C+LGCTRS LNVVA+EKG+VVGR+ F +DGD IDCT+MG+GGKAIPP +D++  +QSDA FILLVEK+AAYMRLAEDRFYN++PCI++TAKGQPDVATRLFL+K+K EL++PVL LVDSDPYGLKILSVY  GSKNMSYDSA+LTT DIKWLG+RPSDLDKY IP QCRL MT+ D++TG+ LL+EDF++KNP WV+EL LM+K K+KAEIQALS+FGFQY++E YLP KL++ DW+
Sbjct:   33 ILQTLKTLKVSSAASSSKTLTLADVGLSTTCREVTDLPIDEVQYNIGSVMLNLARSILAGEGFSFNVPSRSSANQLYVPELDRIILKDKSSVRAFSNVSTVRKTTITTRILQLIHQLCTKNIHVTKRDLFYTDVKLFQDQTQSDAVLDDVSCILGCTRSSLNVVAAEKGVVVGRLIFSDDGDIIDCTKMGMGGKAIPPNIDRVGDMQSDALFILLVEKDAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELNLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDIKTGKDLLEEDFVKKNPAWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQEQDWL 420          

BLAST of EWM25406.1 vs. NCBI_GenBank
Match: gi|697187007|ref|XP_009602544.1| (PREDICTED: DNA topoisomerase 6 subunit A [Nicotiana tomentosiformis] >gi|1025160200|ref|XP_016469099.1| PREDICTED: DNA topoisomerase 6 subunit A-like [Nicotiana tabacum])

HSP 1 Score: 551.206 bits (1419), Expect = 0.000e+0
Identity = 256/388 (65.98%), Postives = 321/388 (82.73%), Query Frame = 0

Query:  187 VLEEVRQLMLRSRRDDAPNPTLADLGI-TTVREVSDMAPEGVLFEIEKTILAAAGMILRGEGFSYQVPTRSATNQLYIAELDRIVLSDKVATRAFLNPREVRKTAITTRIMQLVHEVLSKGIHITKRDMFYTDVKLFRDQKDSDVVLDDVACMLGCTRSCLNVVASEKGLVVGRVQFEEDGDFIDCTRMGVGGKAIPPYVDKIACIQSDAKFILLVEKEAAYMRLAEDRFYNKYPCIVITAKGQPDVATRLFLKKLKTELHIPVLGLVDSDPYGLKILSVYMTGSKNMSYDSASLTTSDIKWLGVRPSDLDKYGIPPQCRLDMTDHDMRTGRQLLQEDFIQKNPEWVKELQLMLKMKKKAEIQALSAFGFQYITEDYLPRKLEKGDWI 573
            +L+ ++ L   S    +   TLAD+G+ TT REV+D+  + V + I   +L  A  IL GEGFS+ VP+RS+ NQLY+ ELDRI+L DK + RAF N   VRKT ITTRI+QL+H++ +K IH+TKRD+FYTDVKLF+DQ  SD VLDDV+C+LGCTRS LNVVA+EKG+VVGR+ F +DGD IDCT+MG+GGKAIPP +D++  +QSDA FILLVEK+AAYMRLAEDRFYN++PCI++TAKGQPDVATRLFL+K+K EL++PVL LVDSDPYGLKILSVY  GSKNMSYDSA+LTT DIKWLG+RPSDLDKY IP QCRL MT+ D++TG+ LL+EDF++KNP WV+EL LM+K K+KAEIQALS+FGFQY++E YLP KL++ DW+
Sbjct:   33 ILQTLKNLKASSAASSSKTLTLADVGLSTTCREVTDLPIDEVQYNIGSVMLNLARSILAGEGFSFNVPSRSSANQLYVPELDRIILKDKSSVRAFSNVSTVRKTTITTRILQLIHQLCTKNIHVTKRDLFYTDVKLFQDQTQSDAVLDDVSCILGCTRSSLNVVAAEKGVVVGRLIFSDDGDMIDCTKMGMGGKAIPPNIDRVGDMQSDALFILLVEKDAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELNLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPDQCRLPMTEQDIKTGKDLLEEDFVKKNPAWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKLQEQDWV 420