EWM25780.1, cds4897 (CDS) Nannochloropsis gaditana

BLAST of EWM25780.1 vs. NCBI_GenBank
Match: gi|585107646|gb|EWM25780.1| (phosphatidylinositol 4-kinase [Nannochloropsis gaditana])

HSP 1 Score: 1523.45 bits (3943), Expect = 0.000e+0
Identity = 738/738 (100.00%), Postives = 738/738 (100.00%), Query Frame = 0

Query:    1 MYSLLPFLVYWLAVQEGLRVGGPGGPEGLRLSSGSRDAFLRDGFPSQHQLGGFDADAMAITPAVFDSQIEECIPQIATLLACTKVDGPPDAMRRNRWIEQQVIEKCTSNVHLGLKLFWLLRAALGHLEIPTRRGLPQITGERQRAHLMSLLNTGEILAREGQQSPGCLRPLRARYFAHCTTFVAALVKLSFDVKGVSSVRRLTALHQGIDRVNALLFRLMHTRGEEMEGGPDEASQSPQTPGSRPSASPPQRHGVSAVTGGGSAGASAHPSWLTEEHIARQCPEVAAHSVHLPMLDGSSPIFRFLRLVSAECELLPSRERCPYMVVAELLQTERNTRCGSARLYTGGATLGCSVWDALDHRVFPESLLYPDDHPAMRYPVRPFVFNNDYNDEDDYFLDLHATPHAPPHHSRSLPHHRPLPPPQAPLPPPLAFDEDTLSAMDPRLLDPSTRRAITVRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGFGVLNQPRHRRRLLDLMAVSIFEVPGKAAILDSFDKRLVYASYAPNALALVDESFDNYRTKMYDSYQKRRNGIW 738
            MYSLLPFLVYWLAVQEGLRVGGPGGPEGLRLSSGSRDAFLRDGFPSQHQLGGFDADAMAITPAVFDSQIEECIPQIATLLACTKVDGPPDAMRRNRWIEQQVIEKCTSNVHLGLKLFWLLRAALGHLEIPTRRGLPQITGERQRAHLMSLLNTGEILAREGQQSPGCLRPLRARYFAHCTTFVAALVKLSFDVKGVSSVRRLTALHQGIDRVNALLFRLMHTRGEEMEGGPDEASQSPQTPGSRPSASPPQRHGVSAVTGGGSAGASAHPSWLTEEHIARQCPEVAAHSVHLPMLDGSSPIFRFLRLVSAECELLPSRERCPYMVVAELLQTERNTRCGSARLYTGGATLGCSVWDALDHRVFPESLLYPDDHPAMRYPVRPFVFNNDYNDEDDYFLDLHATPHAPPHHSRSLPHHRPLPPPQAPLPPPLAFDEDTLSAMDPRLLDPSTRRAITVRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGFGVLNQPRHRRRLLDLMAVSIFEVPGKAAILDSFDKRLVYASYAPNALALVDESFDNYRTKMYDSYQKRRNGIW
Sbjct:    1 MYSLLPFLVYWLAVQEGLRVGGPGGPEGLRLSSGSRDAFLRDGFPSQHQLGGFDADAMAITPAVFDSQIEECIPQIATLLACTKVDGPPDAMRRNRWIEQQVIEKCTSNVHLGLKLFWLLRAALGHLEIPTRRGLPQITGERQRAHLMSLLNTGEILAREGQQSPGCLRPLRARYFAHCTTFVAALVKLSFDVKGVSSVRRLTALHQGIDRVNALLFRLMHTRGEEMEGGPDEASQSPQTPGSRPSASPPQRHGVSAVTGGGSAGASAHPSWLTEEHIARQCPEVAAHSVHLPMLDGSSPIFRFLRLVSAECELLPSRERCPYMVVAELLQTERNTRCGSARLYTGGATLGCSVWDALDHRVFPESLLYPDDHPAMRYPVRPFVFNNDYNDEDDYFLDLHATPHAPPHHSRSLPHHRPLPPPQAPLPPPLAFDEDTLSAMDPRLLDPSTRRAITVRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGFGVLNQPRHRRRLLDLMAVSIFEVPGKAAILDSFDKRLVYASYAPNALALVDESFDNYRTKMYDSYQKRRNGIW 738          

BLAST of EWM25780.1 vs. NCBI_GenBank
Match: gi|545706279|ref|XP_005705457.1| (phosphatidylinositol 4-kinase [Galdieria sulphuraria] >gi|452821912|gb|EME28937.1| phosphatidylinositol 4-kinase [Galdieria sulphuraria])

HSP 1 Score: 362.073 bits (928), Expect = 1.788e-108
Identity = 227/605 (37.52%), Postives = 316/605 (52.23%), Query Frame = 0

Query:  172 RARYFAHCTTFVAALVKLSFDVKGVSSVRRLTALHQGIDRVNALLFRLMHTRGEEMEGGPDEASQSPQTPGSRPSASPPQRHGVSAVTGGGSAGASAHPSWLTEEHIARQCPEVAAHSVHLPMLDGSSPIFRFLRLVSAECELLPSRERCPYMVVAELLQTERNTRCGSARLYTGGATLGCSVWDALDHRVFPESLLYPDDHPAMRYPVRPFVFNNDYNDEDDY-FLDLHATPHAPPHHSRSLPHHRPLPPPQAPLPPPLAFDEDTLSAMDPR---------------------------LLDPST--RRAITVRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGFGVLNQPRHRRRLLDLMAVSI--FEVP---GKAAILDSFDKR----LVYASYAPNALALVDESFDNYRTKMYDSYQKRRNGI 737
            R  YF     FV  LV+LS   +   S +R + + +G+DR+N L  R M      +E  P     SP  P       PPQ                  PS    E +A+Q   VA  S+HLP+    S + R LR V  E  + P+R R P++V  E+++T  N  C             CS +      VF E +L                  +DY + DD   + +  +PH   H +R         PP +      + +  T  +   +                           L DP +   R   +  V+GE+WHWK +RIR +SPFG   GW+LA FIVKAGDDLRQEQ+A+QLI   R IF EE LELW+RP+ +VC+   +GL+E + DA S+  +KKR P+F +L +Y+ER+YGG  S+ FR AQRNF+ S+AGY +  YLLQ+KDRHN NI++D QG +IHIDFGFMLG SPGSI FESAPFKLT E+V+++GG     +Q FR ++V+GF  L   +H  +L  L+ + I   ++P   G  ++L+SF +R    L+      + L+L+DES DN+RT+ YD YQ   NGI
Sbjct:  435 RFDYFNDGLVFVKQLVRLSSAARDAPSDKRYSFIQRGLDRINELFLRRMSG----LESHP-----SPLKP-------PPQL-----------------PSL---EWLAQQGRAVALRSLHLPIARAVSRVLRILRFVPEETIVFPTRTRVPFLVFVEVVET--NMLC-------------CSQY------VFFEHMLV-----------------DDYEENDDKDIVAVMESPHIASHIARKRTLSSEETPPSSKETRSGSVESSTTPSSKQKEHVRNAVYGNSVTAVNMEEYVESGKEDLEDPDSIISRDAALLAVYGEIWHWKAERIRKASPFGHFSGWKLASFIVKAGDDLRQEQLAVQLIAQFRNIFVEEDLELWVRPFTVVCLSADSGLVETLPDAVSLHALKKRTPNFVTLLDYFERVYGGKDSKRFRRAQRNFVESMAGYSLVCYLLQIKDRHNGNIMLDSQGRVIHIDFGFMLGNSPGSIRFESAPFKLTAEFVDVMGGTHSEAFQYFRELMVQGF--LAARKHHEKLTTLVEIMIEGTKIPCMHGGHSVLESFRQRFCLGLLENECIHHMLSLIDESVDNWRTRQYDRYQFYTNGI 963          

BLAST of EWM25780.1 vs. NCBI_GenBank
Match: gi|298705807|emb|CBJ28976.1| (similar to phosphatidylinositol 4-kinase III beta [Ectocarpus siliculosus])

HSP 1 Score: 347.821 bits (891), Expect = 5.164e-106
Identity = 248/694 (35.73%), Postives = 344/694 (49.57%), Query Frame = 0

Query:   66 DSQIEECIPQIATLLACTKVDGPPDAMRRNRWIEQQVI--------EKCTSNVHLGLKLFWLLRAALGHLEIPTRRGLPQITGERQRAHLMSLLNTGEILAREGQQSPGCLRPLRARYFAHCTTFVAALVKLSFDVKGVSSVRRLTALHQGIDRVNALLFRLMHTRGEEMEGGPDEASQSPQTPGSRPSASPPQRHGVSAVTGGGSAGASAHPSWLTEEHIARQCPEVAAHSVHLPM------LDGSSPIFRFLRLVSAECELLPSRERCPYMVVAELLQTERNTRCGSARLYTGGATLGCSVWDALDHRVFPESLLYPDDHPAMRYPVRPFVFNNDYNDE---DDYFLDLHATPHAPPHHSRSLPHHRPLPPPQAPLPPPLAFDEDT---LSAMDPRLLDPSTRRAITVRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGFGVLNQPRHRRRLLDL---MAVSIFEVPGKAAILDSFDKRLVYASYAPNALALVDESFDNYRTKMYDSYQKRRNG 736
            DS +E  +PQ+A +L      G P    RN  I Q V+        ++C  N+ L +++ W         E   RR  PQ  G    A      N+  + A  G   P  LR  R  Y A    FV  L+ +   +K +   RRL+AL   + RVN +L R M TRG   E            PG              A  GGG  G      WL E  +A  CPEV+ H++HLP+         ++   R LRL    C +LPS+E+ PY+VVAE+LQT  +    S RLY  G + G +  D ++ +  P++L        +R   +  V+ +   DE     Y L+L                              L FD  T   L++ DP    P  +      +VFG      E+ +R+ SPFG L+GW+LA FIVK+GDD R E +AMQ+I LV  IFK EGL L LRPY I+C G+  GL+E V DAKSIDHIKK       ++ ++ER+Y G  S  +  A  NF+RSLAGY + TY+LQVKDRHNANI++D +GH+IHIDFG++LG+SPG  + ESAPFK T++Y+E++GG     ++ FR + + GF  +   RH R +  L   M  S         IL  F KR +        + LVD+S  ++ T  YD +Q++ NG
Sbjct:   57 DSDVEFFVPQLAAVLMSK---GVPS---RNVHITQLVVGRLSANLQQRCFGNIGLAVQMMW-------QFEAVGRR--PQEDGPSWNA----FKNSYVLAALNGDNLPEQLRMQRRAYCADSMAFVDTLMDIGDSLKKLPIKRRLSALDNELGRVNRMLLRRMQTRGASFE-----------IPGD-----------TVAGGGGGGGGGGGGREWLNEHDVAAVCPEVSHHAIHLPIKVLKPGQPAATRAVRVLRLCRELCRVLPSKEKAPYLVVAEVLQTSFSY--ASDRLYLEGGSAGVTCEDVVNKKTLPQAL----QEELLRSRGQD-VWEDQNQDEASSQSYQLELEEAD-------------------------CLFFDRATNQYLTSGDPS--SPHIKNMREALQVFGTTSAALERMVRHQSPFGHLKGWKLAHFIVKSGDDFRMEALAMQVIRLVDSIFKREGLSLRLRPYSILCCGEMKGLVECVTDAKSIDHIKKFTAKSGGIRTFFERMYMGKTSGLYGRAVDNFVRSLAGYAIVTYMLQVKDRHNANIMLDTRGHIIHIDFGYILGSSPGWWNHESAPFKFTQDYMEIMGGPGSDKYERFRELFLTGFKAVQ--RHMREIETLIWPMFPSSDHRMTTQQIL--FRKRFMDFQTDDEIMRLVDDSVRSWTTGQYDDFQQKTNG 671          

BLAST of EWM25780.1 vs. NCBI_GenBank
Match: gi|544211798|ref|XP_005536298.1| (phosphatidylinositol 4-kinase [Cyanidioschyzon merolae strain 10D] >gi|449016610|dbj|BAM80012.1| phosphatidylinositol 4-kinase [Cyanidioschyzon merolae strain 10D])

HSP 1 Score: 302.368 bits (773), Expect = 4.263e-85
Identity = 157/325 (48.31%), Postives = 212/325 (65.23%), Query Frame = 0

Query:  424 APLPPPLAFDEDTLSAMDPRLLDPSTRRA--ITVRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGFGVLNQPRHRRRLLDLMAVSI--FEVP---GKAAILDSFDKRLVYA----SYAPNALALVDESFDNYRTKMYDSYQKRRNGI 737
            A +P  + F E   + +D    + S RRA   T+  V+GE+W WKE R+R +SPFG L  WRLA FIVKAGDDLRQEQ+A+QLI  +  IF+EE L LWLRP+ IVC+G+ AGL+E + DA S+  +KKR P+F SL+EY+ER YG P S    +A RNF  S+AGY + TYL QV+DRHN NI++   GH+IHIDFGFMLG SPGSI FE+APFKL+ EY++++G  +   +  FR + V GF  L   +H  +L  L+ + +    +P   G  A++D   +RL+ +          L L+DES DN+RT+ YD +Q   NGI
Sbjct:  856 AHVPRGVNFREQMKAPLD----EASERRAREATLLSVYGEMWAWKEARVRLASPFGHLPTWRLASFIVKAGDDLRQEQLAVQLIEQMLRIFEEESLPLWLRPFTIVCLGNNAGLVETIPDAVSVHALKKRTPNFISLREYFERAYGAPPSPALAKAVRNFTESMAGYSLVTYLFQVRDRHNGNIMLAASGHVIHIDFGFMLGNSPGSIRFEAAPFKLSPEYLQVMGDVQSETFCYFRELFVSGF--LACRKHHEKLTTLIEIMLEGTRLPCMAGGMAVVDGLRQRLMLSLPERECIRAVLDLIDESIDNWRTRQYDRFQFYSNGI 1174          

HSP 2 Score: 68.1662 bits (165), Expect = 5.459e-8
Identity = 49/173 (28.32%), Postives = 78/173 (45.09%), Query Frame = 0

Query:  172 RARYFAHCTTFVAALVKLSFDVKGVSSVRRLTALHQGIDRVNALLFRLMHTRGEEMEGGPDEASQSPQTPGSRPSASPPQRHGVSAVTGGGSAGASAHPSWLTEEHIARQCPEVAAHSVHLPMLDGSSPIFRFLRLVSAECELLPSRERCPYMVVAELLQTERNTRCGSARLY 344
            R  YF     F  +LV++S D++ +S  +R   L   ++ ++ L++R    R    EG              RPS    Q   VSA                    +A +CP  A  S+HLP+   +  + R LR+ S EC +L S++R P+MV AE+L+T  N +C    ++
Sbjct:  459 RFDYFNDMLYFFRSLVRISLDLRAISPSKRERILRSRLEDLSELIYR----RIAGFEG--------------RPSRLGAQDIEVSAAM------------------VAPRCPRAALRSIHLPLSRSNESVLRLLRIASIECVILSSKDRVPFMVYAEVLET--NMKCSDRNIF 593          

BLAST of EWM25780.1 vs. NCBI_GenBank
Match: gi|1189386091|gb|OSX73221.1| (hypothetical protein BU14_0368s0021 [Porphyra umbilicalis])

HSP 1 Score: 278.485 bits (711), Expect = 1.565e-76
Identity = 139/306 (45.42%), Postives = 198/306 (64.71%), Query Frame = 0

Query:  451 RAITVRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQ----------EDMNWQEFRSMVVRGFGVLNQPRHRRRLLDLMAV-----SIFEVPGKAAILDSFDKRLVY----ASYAPNALALVDESFDNYRTKMYDSYQKRRNGI 737
            R   +  V+GELW WKE+R+  +SPFG++ G +L  FIVKAGDDLRQEQ+A+QLI     IFKEE L+L+LRP+ ++CV   +GLIE +ADA S+  +KKR P+F+SL +Y+ER YG   ++ F +AQR F++S+A Y V TYLLQ+KDRHN NI++D  G ++HIDFG++L  SPG+I FESAPFKLT E++++L G+          +    + FR + V GF  L   +H  RL  L+ +     S+  + G  A+++   +R       +    N L L+ ES  N R+K+YDS+Q   NGI
Sbjct:  923 RQAALLAVYGELWAWKEERVLRASPFGKMHGAKLVPFIVKAGDDLRQEQLAIQLIAQFDAIFKEESLDLYLRPFTVMCVSADSGLIETIADAVSLHVLKKRTPNFSSLLDYFERAYGKVGTQGFHQAQRRFIQSMAAYSVVTYLLQIKDRHNGNIMLDANGRIVHIDFGYILSNSPGAIKFESAPFKLTDEFIDVLAGRPGAADSAGFADTPGCRYFRELCVLGF--LAARKHSDRLTTLVEIMLEGTSMPCMTGGLAVVEGLRRRFFLGVPESQCVSNVLGLIAESTSNSRSKLYDSFQYLTNGI 1226          

BLAST of EWM25780.1 vs. NCBI_GenBank
Match: gi|290980661|ref|XP_002673050.1| (predicted protein [Naegleria gruberi] >gi|284086631|gb|EFC40306.1| predicted protein [Naegleria gruberi])

HSP 1 Score: 263.077 bits (671), Expect = 3.046e-73
Identity = 164/480 (34.17%), Postives = 251/480 (52.29%), Query Frame = 0

Query:  288 HSVHLPMLDGSSPIFRFLRLVSAECELLPSRERCPYMVVAELLQTERNTRCGSARLYTGGATLGCSVWDALDHRVFPESLLYPDDHPAM-------RYPVRPFVFN---------NDYNDEDDYFLDLHATPHAPPHHS--RSLPHHRPLPPPQAPLPPPLAFDEDTLSAMDPRLLDPSTRRAI--TVRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGFGVLNQPRHRRRLLDLMAVSI------FEVPGKAAILDSFDKRLV----YASYAPNALALVDESFDNYRTKMYDSYQKRRNGI 737
            + V+ P+ +   P  R LR+ +  C +  ++ER P + V E+L  +         +     T   +++D +  +   +      D PA        R     + F+         ND  DE D    +  +  + P  S   S+P        + P+      ++D    ++ +  DP+    +   +  VFGE +   + + R  SP+G   GW L   IVKA DD+RQE  AMQLI L   IF+EE L +++RPY+I+      G++E V D  SID +KK+ P+FT+LK+Y+ R+YG P S TF  AQ NF+ S+ GY + +Y+LQ+KDRHN NIL+D++GH++HIDFGF LG SPG + FE++PFKL +++V+L+G   D++   FR +    F  L +PR+  RLL+L+ + I      F+    A I+     R         +A  A  LVD+S DN+ TK YD YQ   NGI
Sbjct:  313 YGVYYPLCESRDPHCRILRICTDACRVFSTKERVPILCVFEVLTGDAPCTQPEDVIQFDETTEEFNIYDGIIVKKNGDCT----DSPATEESRMIERRISSTYQFDVTMDDEKHKNDILDEIDATSTISDSSDSGPRKSDASSIPQTPSTFKSELPILHIPNINQDDFVLIEKK--DPTRDEEVLHDLESVFGESFEKIKAKYRAVSPYGCAPGWDLRSVIVKANDDIRQEIFAMQLIKLFARIFEEEKLPIYIRPYEIIVTSGNCGMLETVRDTCSIDGLKKKFPNFTNLKDYFIRVYGSPTSNTFIRAQTNFVESVVGYSLISYILQIKDRHNGNILLDREGHMVHIDFGFFLGISPGGVKFENSPFKLPQDWVDLMGELFDLH---FRPVFYVAFAALRKPRNMNRLLNLVEMMIGANYKCFD-GNHANIMRDLRNRFYPNQSEEEFAKTARKLVDDSIDNWFTKRYDQYQYLTNGI 782          

BLAST of EWM25780.1 vs. NCBI_GenBank
Match: gi|831763605|ref|XP_012750922.1| (hypothetical protein SAMD00019534_090540 [Acytostelium subglobosum LB1] >gi|735851554|dbj|GAM25879.1| hypothetical protein SAMD00019534_090540 [Acytostelium subglobosum LB1])

HSP 1 Score: 249.21 bits (635), Expect = 3.067e-72
Identity = 120/250 (48.00%), Postives = 169/250 (67.60%), Query Frame = 0

Query:  455 VRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGF-GVLNQPRHRRRLLDLMA-VSIFEVPGKAAILDSFDKRL 702
            +  +FGE W  KE +I  +SP+  + GWRL  FIVK+GDDLRQEQ+AMQLIT    IFK+  L LWLRPY +V  G  +GLIE + D+ S+D +KK+ P++T+L  Y+   YG   S+ F  AQ NF+ S+AGY +  Y LQ+KDRHN NILID++GH+IHID+GF+L  SPG+I+FE APFKLT+E VEL+GG++   ++ F  +++ GF  V N  +    +++LM   +  +   +  +LDSF  R 
Sbjct:   99 LNTIFGENWKTKENKIWRTSPYRSISGWRLISFIVKSGDDLRQEQLAMQLITQFYRIFKKTNLPLWLRPYLVVATGQDSGLIETIPDSTSLDTLKKKCPNYTTLLNYFIDAYGDNRSKEFILAQSNFIESVAGYALVCYFLQIKDRHNGNILIDRKGHIIHIDYGFLLSTSPGAINFEKAPFKLTEEIVELMGGEKSDRFKYFTVLLLSGFLQVRNHYQEFMMMIELMIPHTNLKCLKREGVLDSFRARF 348          

BLAST of EWM25780.1 vs. NCBI_GenBank
Match: gi|66808077|ref|XP_637761.1| (phosphatidylinositol 4-kinase [Dictyostelium discoideum AX4] >gi|60466201|gb|EAL64264.1| phosphatidylinositol 4-kinase [Dictyostelium discoideum AX4])

HSP 1 Score: 265.003 bits (676), Expect = 5.906e-72
Identity = 136/292 (46.58%), Postives = 183/292 (62.67%), Query Frame = 0

Query:  455 VRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGFGVLNQPRHR-RRLLDLMAVSI---FEVPGKAAILDSFDKRL-----VYASYAPNALALVDESFDNYRTKMYDSYQKRRNGI 737
            +  +FGELW  KE RI  +S     +GW L  FIVK+GDDLRQEQ AMQLIT    IFK+  L LWLRPY ++  G  +GLIE V D  S+D++KK+   FT+L +YY R YG   S++F +AQ  F+ SLAGY +  Y LQ+KDRHN NILID +GHLIHID+GF+   SPGS++FE APFKLTKE+++L+GG E   ++ F  ++V GF  + +     + +++L+       F V    + LDSF  R               LAL+++S +N+ T  YDSYQ   NGI
Sbjct:  874 IYTIFGELWKQKENRIYKTSMMSHHDGWGLISFIVKSGDDLRQEQFAMQLITQFHRIFKQCRLPLWLRPYLVIATGHDSGLIETVPDTISLDNLKKKDTAFTTLLDYYIRCYGDVNSKSFAKAQACFIESLAGYSLVCYFLQIKDRHNGNILIDTRGHLIHIDYGFIFSTSPGSMNFEKAPFKLTKEFIDLMGGVESDRFEYFTKLLVAGFVEIKKHYEEFKMMIELLLPHTNLSFIVKAGESSLDSFKDRFKSNLTTVEQCEQFVLALINQSLNNWTTASYDSYQYYTNGI 1165          

BLAST of EWM25780.1 vs. NCBI_GenBank
Match: gi|330846412|ref|XP_003295026.1| (hypothetical protein DICPUDRAFT_160141 [Dictyostelium purpureum] >gi|325074377|gb|EGC28449.1| hypothetical protein DICPUDRAFT_160141 [Dictyostelium purpureum])

HSP 1 Score: 263.077 bits (671), Expect = 7.769e-72
Identity = 134/292 (45.89%), Postives = 185/292 (63.36%), Query Frame = 0

Query:  455 VRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLGGQEDMNWQEFRSMVVRGFGVLNQPRHR-RRLLDLM---AVSIFEVPGKAAILDSFDKRL-----VYASYAPNALALVDESFDNYRTKMYDSYQKRRNGI 737
            +  +FGELW  KE RI  SS      GW L  FIVK+GDDLRQEQ AMQ+IT    IF++  L LWLRPY ++  G  +GLIE V D  S+D++KK+   FT+L +Y+ R YG   S++F++AQ  F+ SLAGY +  Y LQ+KDRHN NILID +GHLIHID+GF+L  SPG+++FE APFKLT+E+++L+GG E   ++ F  ++V GF  + +     + +LDL+       F V G  A + SF +R             + ++L+  S +N+ T  YDSYQK  NGI
Sbjct:  708 IYAIFGELWKQKENRIYKSSMMSHHPGWGLISFIVKSGDDLRQEQFAMQMITQFHRIFRKSKLPLWLRPYLVIATGHDSGLIETVPDTISLDNLKKKDTAFTNLYDYFIRAYGDVNSKSFKKAQTGFIESLAGYSLVCYFLQIKDRHNGNILIDTRGHLIHIDYGFLLSTSPGNMNFEKAPFKLTQEFIDLMGGVESDCFKYFTYLLVAGFVEIRKHYEEFKMILDLLLPQTNLTFFVKGGPASITSFKERFKLDLKSVEECEAHMMSLISLSINNWTTASYDSYQKLTNGI 999          

BLAST of EWM25780.1 vs. NCBI_GenBank
Match: gi|255088872|ref|XP_002506358.1| (predicted protein [Micromonas commoda] >gi|226521630|gb|ACO67616.1| predicted protein [Micromonas commoda])

HSP 1 Score: 245.358 bits (625), Expect = 4.140e-71
Identity = 146/354 (41.24%), Postives = 202/354 (57.06%), Query Frame = 0

Query:  397 LDLHATPHA-PPHHSRSLPHHRPLPPPQAPLPPPLAFDEDTLSAMDPRLLDPSTRRAITVRKVFGELWHWKEQRIRNSSPFGRLEGWRLAMFIVKAGDDLRQEQVAMQLITLVREIFKEEGLELWLRPYDIVCVGDQAGLIEAVADAKSIDHIKKRAPHFTSLKEYYERIYGGPYSRTFREAQRNFMRSLAGYCVCTYLLQVKDRHNANILIDKQGHLIHIDFGFMLGASPGSISFESAPFKLTKEYVELLG----GQEDMNWQEFRSMVVRGFGVLNQPRHRRR---LLDLMAVS---IFEVPGKA--AILDSFDKRLVYASYAPNALALVDESFDNYRTKMYDSYQKRRNGI 737
            LD  A P A  P   RS    RP     APL P         S   P++  P  R A       GE W  K +R+R+SSP+G L GW L   I+KAGD+ RQE +A+QL    R+I++E GL LW+RP++++     +  IEAV DA SI  IK RAP  TSL++++E  +G P    FR+AQRNF+ SLAGY + TY+LQVKDRHN NIL+   GHLIHIDF FML  SPG I+FES+PFKLT+EY+E++     G     +  ++ + ++G+  L+  +H  R   L+++M+ S    F+   K    +   F+            L L+ +S D + T+ YD YQ+  NGI
Sbjct:   16 LDATAAPPALTPTRPRSADASRP-----APLEPG--------SPTTPQIPSPWKRSA----GGLGEKWCDKVERVRSSSPYGTLPGWSLKPVIIKAGDNCRQELLAIQLTQQFRQIYRESGLPLWIRPFEVIAQSTTSAFIEAVTDAPSIHAIKSRAPRGTSLRQHFEARFGAPGQPRFRQAQRNFVESLAGYSILTYILQVKDRHNGNILLHDDGHLIHIDFNFMLSTSPGGINFESSPFKLTREYLEVMDSDDTGAASEAFNYYKVLCIQGY--LSVRKHAERIVQLVEMMSASGCPCFKAGPKVLQNLRRRFNMECTEEQCVEIMLGLISDSMDAWCTRQYDFYQRVLNGI 350