EWM25809.1, cds4859 (CDS) Nannochloropsis gaditana

BLAST of EWM25809.1 vs. NCBI_GenBank
Match: gi|585107676|gb|EWM25809.1| (folylpolyglutamate mitochondrial [Nannochloropsis gaditana])

HSP 1 Score: 1275.77 bits (3300), Expect = 0.000e+0
Identity = 618/618 (100.00%), Postives = 618/618 (100.00%), Query Frame = 0

Query:    1 MDMTSQDYITSIAALNSTQLTGDALHRANNSRNSTMEDMHVYLQRLGLAESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAPGAELHVVKPLPGDVKLGIAGDFQAMNAALALALVREYNVYSRRCSRSTIEGSVAEGIVQDPRAESCVAWVDVYPYISTDRPAVSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQALSRPRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCPVDCAKPTLSRARSARALLLEHNEGFSHARQLDQSVLHALCDAVENMNAQNSSNTPSLLWQQTLRDTWRILSAYRLFAQDKESENESISGIGFSDSNAEVREGGGHGGRVTKWSSWIELADQQTRSKVFPSVAAAMGEIDAMTNIEPFDVLVTGSLYLVGNVLNYVERIPSPFSNSKKK 618
            MDMTSQDYITSIAALNSTQLTGDALHRANNSRNSTMEDMHVYLQRLGLAESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAPGAELHVVKPLPGDVKLGIAGDFQAMNAALALALVREYNVYSRRCSRSTIEGSVAEGIVQDPRAESCVAWVDVYPYISTDRPAVSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQALSRPRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCPVDCAKPTLSRARSARALLLEHNEGFSHARQLDQSVLHALCDAVENMNAQNSSNTPSLLWQQTLRDTWRILSAYRLFAQDKESENESISGIGFSDSNAEVREGGGHGGRVTKWSSWIELADQQTRSKVFPSVAAAMGEIDAMTNIEPFDVLVTGSLYLVGNVLNYVERIPSPFSNSKKK
Sbjct:    1 MDMTSQDYITSIAALNSTQLTGDALHRANNSRNSTMEDMHVYLQRLGLAESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAPGAELHVVKPLPGDVKLGIAGDFQAMNAALALALVREYNVYSRRCSRSTIEGSVAEGIVQDPRAESCVAWVDVYPYISTDRPAVSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQALSRPRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCPVDCAKPTLSRARSARALLLEHNEGFSHARQLDQSVLHALCDAVENMNAQNSSNTPSLLWQQTLRDTWRILSAYRLFAQDKESENESISGIGFSDSNAEVREGGGHGGRVTKWSSWIELADQQTRSKVFPSVAAAMGEIDAMTNIEPFDVLVTGSLYLVGNVLNYVERIPSPFSNSKKK 618          

BLAST of EWM25809.1 vs. NCBI_GenBank
Match: gi|765170123|ref|XP_011471535.1| (folylpolyglutamate synthase, mitochondrial isoform X1 [Oryzias latipes])

HSP 1 Score: 261.536 bits (667), Expect = 1.136e-75
Identity = 166/455 (36.48%), Postives = 237/455 (52.09%), Query Frame = 0

Query:    3 MTSQDYITSIAALNSTQLTGDALHRANNSRN---STMEDMHVYLQRLGL-AESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAP---GAELHVVKPLPGDVKLGIAGDFQAMNAALALALVREYNVYSRRCSRSTIEGSVAEGIVQDPRAESCVAWVDVYPYISTDRPA--VSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQALSR--------PRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCP 440
            +   +Y  +I  LN+ Q    AL +    RN     ++ M  +L+R GL  E L +L I+HV G+KGKGSTCAF E +L   G+RT  ++SPHLV+V ERIR+NG+P+    ++ +FW ++ RL++                   S  ASG +          P YFR LT+LAF V + E+VDV ++EVG+GG +D TN++ KP  C + +L ++HT +LG TIE+IAW+KGGI KPG P F   QP E   ++LR  RA  +  P      L   +     + LG+AG  Q  NA+LAL L   +    RRC  S ++ S++    ++ R +   A+          RP+  +  GL +  WPGR Q L  G   ++F+DGAHTV S+Q+ V WFKG A            R LLF     + +  +L  LV             F  A FCP
Sbjct:   43 LPGMEYQDAICTLNTLQTNASALQQVRQERNHPQQQLQAMQGFLERAGLTVEELNRLNIIHVTGTKGKGSTCAFTEKILRSYGLRTGFYSSPHLVQVRERIRINGQPIGKDIFTKYFWQIYGRLEE-------------------SKPASGGTM---------PAYFRFLTILAFHVFLQEKVDVAVIEVGIGGAYDCTNIIRKPWVCGVASLGIDHTQILGDTIEKIAWQKGGIFKPGVPAFTVSQP-EGAMAVLRD-RAKEIQCPLWVCPNLDEYQTDGTALHLGLAGKHQRWNASLALQLSNSW--LKRRC--SPVDQSLSAINPENTRVQPATAF----------RPSSEMMMGLANTEWPGRNQTLKNG-EVTYFLDGAHTVRSMQACVDWFKGSAAQHEKNASGPVARVLLFNATGDRDSAAMLKLLVP----------CHFDFAVFCP 442          

BLAST of EWM25809.1 vs. NCBI_GenBank
Match: gi|765170127|ref|XP_011471537.1| (folylpolyglutamate synthase, mitochondrial isoform X3 [Oryzias latipes])

HSP 1 Score: 257.684 bits (657), Expect = 2.980e-75
Identity = 168/462 (36.36%), Postives = 240/462 (51.95%), Query Frame = 0

Query:    7 DYITSIAALNSTQLTGDALHRANNSRN---STMEDMHVYLQRLGL-AESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAP---GAELHVVKPLPGDVKLGIAGDFQAMNAALALALVREYNVYSRRCSRSTIEGSVAEGIVQDPRAESCVAWVDVYPYISTDRPA--VSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQALSR--------PRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCPVDCAKPTLSRA 451
            +Y  +I  LN+ Q    AL +    RN     ++ M  +L+R GL  E L +L I+HV G+KGKGSTCAF E +L   G+RT  ++SPHLV+V ERIR+NG+P+    ++ +FW ++ RL++                   S  ASG +          P YFR LT+LAF V + E+VDV ++EVG+GG +D TN++ KP  C + +L ++HT +LG TIE+IAW+KGGI KPG P F   QP E   ++LR  RA  +  P      L   +     + LG+AG  Q  NA+LAL L   +    RRC  S ++ S++    ++ R +   A+          RP+  +  GL +  WPGR Q L  G   ++F+DGAHTV S+Q+ V WFKG A            R LLF     + +  +L  LV      +  P S   L   C    +   L RA
Sbjct:   47 EYQDAICTLNTLQTNASALQQVRQERNHPQQQLQAMQGFLERAGLTVEELNRLNIIHVTGTKGKGSTCAFTEKILRSYGLRTGFYSSPHLVQVRERIRINGQPIGKDIFTKYFWQIYGRLEE-------------------SKPASGGTM---------PAYFRFLTILAFHVFLQEKVDVAVIEVGIGGAYDCTNIIRKPWVCGVASLGIDHTQILGDTIEKIAWQKGGIFKPGVPAFTVSQP-EGAMAVLRD-RAKEIQCPLWVCPNLDEYQTDGTALHLGLAGKHQRWNASLALQLSNSW--LKRRC--SPVDQSLSAINPENTRVQPATAF----------RPSSEMMMGLANTEWPGRNQTLKNG-EVTYFLDGAHTVRSMQACVDWFKGSAAQHEKNASGPVARVLLFNATGDRDSAAMLKLLVD----AAVLPLSLVILTLPCSAQTSLKPLCRA 459          

BLAST of EWM25809.1 vs. NCBI_GenBank
Match: gi|765170125|ref|XP_011471536.1| (folylpolyglutamate synthase, mitochondrial isoform X2 [Oryzias latipes])

HSP 1 Score: 259.61 bits (662), Expect = 6.508e-75
Identity = 166/451 (36.81%), Postives = 235/451 (52.11%), Query Frame = 0

Query:    7 DYITSIAALNSTQLTGDALHRANNSRN---STMEDMHVYLQRLGL-AESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAP---GAELHVVKPLPGDVKLGIAGDFQAMNAALALALVREYNVYSRRCSRSTIEGSVAEGIVQDPRAESCVAWVDVYPYISTDRPA--VSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQALSR--------PRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCP 440
            +Y  +I  LN+ Q    AL +    RN     ++ M  +L+R GL  E L +L I+HV G+KGKGSTCAF E +L   G+RT  ++SPHLV+V ERIR+NG+P+    ++ +FW ++ RL++                   S  ASG +          P YFR LT+LAF V + E+VDV ++EVG+GG +D TN++ KP  C + +L ++HT +LG TIE+IAW+KGGI KPG P F   QP E   ++LR  RA  +  P      L   +     + LG+AG  Q  NA+LAL L   +    RRCS    + S++    ++ R +   A+          RP+  +  GL +  WPGR Q L  G   ++F+DGAHTV S+Q+ V WFKG A            R LLF     + +  +L  LV             F  A FCP
Sbjct:   47 EYQDAICTLNTLQTNASALQQVRQERNHPQQQLQAMQGFLERAGLTVEELNRLNIIHVTGTKGKGSTCAFTEKILRSYGLRTGFYSSPHLVQVRERIRINGQPIGKDIFTKYFWQIYGRLEE-------------------SKPASGGTM---------PAYFRFLTILAFHVFLQEKVDVAVIEVGIGGAYDCTNIIRKPWVCGVASLGIDHTQILGDTIEKIAWQKGGIFKPGVPAFTVSQP-EGAMAVLRD-RAKEIQCPLWVCPNLDEYQTDGTALHLGLAGKHQRWNASLALQLSNSW--LKRRCSP---DQSLSAINPENTRVQPATAF----------RPSSEMMMGLANTEWPGRNQTLKNG-EVTYFLDGAHTVRSMQACVDWFKGSAAQHEKNASGPVARVLLFNATGDRDSAAMLKLLVP----------CHFDFAVFCP 441          

BLAST of EWM25809.1 vs. NCBI_GenBank
Match: gi|1003969453|ref|XP_015734822.1| (PREDICTED: folylpolyglutamate synthase, mitochondrial isoform X2 [Coturnix japonica])

HSP 1 Score: 259.225 bits (661), Expect = 1.087e-74
Identity = 173/458 (37.77%), Postives = 239/458 (52.18%), Query Frame = 0

Query:    7 DYITSIAALNSTQLTGDALHRANNSRNS---TMEDMHVYLQRLGL-AESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRAC--------VLDAPGAELHVVKPLPGDVKLGIAGDFQAMNAALALALVREYNVYSRRCSRSTIEGSVAEGIVQD--PRAE-SCVAWVDVYP-YISTDRPAVSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQAL--------SRPRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCP 440
            DY  +I  LN+ Q     L +    R      ++ M  +L+R GL  E L +L I+HV G+KGKGS CAF E +L   G+RT  ++SPHLV+V ERIR+NG+PL+ + +S +FW V+ RL+     G +  A A++                       P YFR LT++AF V + E+VD+ ++EVG+GG FD TN++  PV C +++L ++HT +LG T+E+IAW+KGGI KPG P F  PQP +    +    R C         LDA  A + V       ++LG+AG  Q  NAALAL L R +        R   EGS    ++QD  P AE S    V + P +  +D  A+ QGL+   WPGR Q L  G   +++IDGAHT  SIQ+ V WF+  AL        S  R LLF     +    LL  L+             F  A FCP
Sbjct:   35 DYQDAIRTLNTLQTNASYLEQVKRERGDPRVQLDAMRGFLERSGLRVEDLDRLNIIHVTGTKGKGSACAFTERILRGYGLRTGFYSSPHLVQVRERIRINGQPLSKELFSKYFWLVYRRLR-----GSKDEAQANM-----------------------PAYFRFLTIMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRTPVVCGVSSLGIDHTSILGDTVEKIAWQKGGIFKPGVPAFTVPQPEQPLEVLRDRARECGCPLYLCPELDAFEAGMRV-------LELGLAGSHQRSNAALALQLSRTW------LQRRGYEGS---DVLQDVLPGAELSAQRSVPLAPTFCPSD--AMIQGLRDTEWPGRTQVLQHG-PVTWYIDGAHTTSSIQACVRWFRQAALNEDKPQDGSEVRVLLFNATGDRDTAALLKLLLP----------CHFDYAVFCP 435          

BLAST of EWM25809.1 vs. NCBI_GenBank
Match: gi|296482024|tpg|DAA24139.1| (TPA: folylpolyglutamate synthase, mitochondrial [Bos taurus])

HSP 1 Score: 254.218 bits (648), Expect = 1.413e-74
Identity = 154/410 (37.56%), Postives = 224/410 (54.63%), Query Frame = 0

Query:    2 DMTSQDYITSIAALNSTQLTGDALHRANNSRNSTMEDMHVYLQRLGL-AESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAPGAELHVVKPLPG------DVKLGIAGDFQAMNAALALALVREY-------NVYSRRCSRSTIEGSVAEGIVQDPRAESCVAWVDVYPYISTDRPAVSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQALSR 397
            DM  QD + ++  L +     + + R      + +E M +YL R GL  E L +L I+H+ G+KGKGSTCAF E +L   G++T  F+SPHLV+V ERIR+NG+P++ + ++ HFW +++RL++ +                 SS  S             PGYFR LTL+AF V + E+VD+ +LEVG+GG +D TN++ KPV C IT+L ++HT LLG T+E+IAW+KGGI K G P F   QP E   ++LR  RA  +  P   L++  PL         + LG+ G+ Q  NAALAL + R +       +    + SR ++ G +    V  P                   P + QGL+   WPGR Q L  G   ++++DGAHT  S+Q+ V WF+ QAL R
Sbjct:   39 DMEYQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLEETKD---------------DSSCVS------------MPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDE-PLAVLRD-RAQQISCP---LYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAPVFQP------------------TPRMQQGLRHTEWPGRTQLLRRG-PLTWYLDGAHTTSSMQACVRWFR-QALHR 396          

BLAST of EWM25809.1 vs. NCBI_GenBank
Match: gi|1201843030|ref|XP_021269287.1| (folylpolyglutamate synthase, mitochondrial isoform X2 [Numida meleagris])

HSP 1 Score: 257.684 bits (657), Expect = 2.671e-74
Identity = 169/452 (37.39%), Postives = 237/452 (52.43%), Query Frame = 0

Query:   11 SIAALNSTQLTGDALHRANNSRN---STMEDMHVYLQRLGL-AESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAPGAELHVVKPLPG------DVKLGIAGDFQAMNAALALALVREYNVYSRRCSRSTIEGSVAEGIVQD--PRAE-SCVAWVDVYP-YISTDRPAVSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQAL--------SRPRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCP 440
            +I  LN+ Q     L +    R    + +E M  +L+R GL  E L +L I+HV G+KGKGS CAF E +L   G+RT  ++SPHLV+V ERIR+NG+PL+ + +S +FW V+ RLQD +       A A++                       P YFR LT++AF V + E+VD+ ++EVG+GG FD TN++  PV C +++L ++HT +LG T+E+IAW+KGGI KPG P F   QP +    +    R C     G  L++   L         ++LG+AG  Q  NAALAL L R +        R   EG+   G++QD  P AE S    V + P +  +D  A+ QGL+   WPGR Q L  G   +++IDGAHT  S+Q+ V WF+  AL        S  R LLF     +    LL  L+             F  A FCP
Sbjct:   23 AIRTLNTLQTNASYLEQVKRERGDPRAQLEAMRGFLERSGLRVEDLDRLNIIHVTGTKGKGSACAFTERILRGYGLRTGFYSSPHLVQVRERIRINGQPLSKELFSKYFWLVYRRLQDTKD-----KAQANM-----------------------PAYFRFLTIMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRAPVVCGVSSLGIDHTSILGDTVEKIAWQKGGIFKPGVPAFTVQQPEQPLEVLRDRAREC-----GCPLYLCPELDAFETGTRVLELGLAGSHQRSNAALALQLSRTW------LQRRGYEGT---GVLQDVLPSAELSAQRSVPLAPTFCPSD--AMIQGLRDTEWPGRTQVLPHG-PVTWYIDGAHTTSSVQACVRWFRQAALNEDKPQDGSEVRVLLFNATGDRDTAALLKLLLP----------CHFDYAVFCP 419          

BLAST of EWM25809.1 vs. NCBI_GenBank
Match: gi|971423562|ref|XP_015135198.1| (PREDICTED: folylpolyglutamate synthase, mitochondrial isoform X3 [Gallus gallus])

HSP 1 Score: 258.07 bits (658), Expect = 2.790e-74
Identity = 170/456 (37.28%), Postives = 239/456 (52.41%), Query Frame = 0

Query:    7 DYITSIAALNSTQLTGDALHRANNSRN---STMEDMHVYLQRLGL-AESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAPGAELHVVKPLPG------DVKLGIAGDFQAMNAALALALVREYNVYSRRCSRSTIEGSVAEGIVQD--PRAE-SCVAWVDVYP-YISTDRPAVSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQAL--------SRPRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCP 440
            DY  +I  LN+ Q     L +    R    + +E M  +L+R GL  E L +L I+HV G+KGKGS CAF E +L   G+RT  ++SPHLV+V ERIR+NG+PL+ + +S +FW V+ RL+     G +  + A++                       P YFR LT++AF V + E+VD+ ++EVG+GG FD TN++  PV C +++L ++HT +LG T+E+IAW+KGGI KPG P F  PQP +    +    R C     G  L++   L         ++LG+AG  Q  NAALAL L R +        R   EG+    ++QD  P AE S    V + P +  +D  A+ QGL+   WPGR Q L  G   +++IDGAHT  SIQ+ V WF+  AL        S  R LLF     +    LL  L+             F  A FCP
Sbjct:   35 DYQDAIRTLNTLQTNASYLEQVKRERGDPRAQLEAMRGFLERSGLQVEDLDRLNIIHVTGTKGKGSACAFTERILRGYGLRTGFYSSPHLVQVRERIRINGQPLSKELFSKYFWLVYRRLR-----GTKDESQANM-----------------------PAYFRFLTIMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRAPVVCGVSSLGIDHTSILGDTVEKIAWQKGGIFKPGVPAFTVPQPEQPLEVLRDRAREC-----GCPLYLCPELDAFEAGTRVLELGLAGSHQRSNAALALQLSRTW------LQRRGYEGT---DVLQDVLPGAELSAQRSVPLAPTFCPSD--AMIQGLRDTEWPGRTQVLPHG-PVTWYIDGAHTTSSIQACVRWFRQAALNEDKPQDGSEVRVLLFNATGDRDTAALLKLLLP----------CHFDYAVFCP 435          

BLAST of EWM25809.1 vs. NCBI_GenBank
Match: gi|1187553370|ref|XP_020730525.1| (folylpolyglutamate synthase, mitochondrial [Odocoileus virginianus texanus])

HSP 1 Score: 258.07 bits (658), Expect = 3.011e-74
Identity = 167/460 (36.30%), Postives = 240/460 (52.17%), Query Frame = 0

Query:    7 DYITSIAALNSTQLTGDALHRANNSR---NSTMEDMHVYLQRLGL-AESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAPGAELHVVKPLPG------DVKLGIAGDFQAMNAALALALVREY-------NVYSRRCSRSTIEGSVAEGIVQDPRAESCVAWVDVYPYISTDRPAVSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQAL---------SRPRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCP 440
            +Y  ++  LN+ Q   + L      R    + +E M +YL R GL  E L +L I+H+ G+KGKGSTCAF E +L   G++T  F+SPHLV+V ERIR+NG+P++ + ++ HFW++++RL++ +                 SS  S             PGYFR LTL+AF V + E+VD+ ++EVG+GG +D TN++ KPV C IT+L ++HT LLG T+E+IAW+KGGI K G P F  PQP E   ++LR  RA  +  P   L++  PL         + LG+ G+ Q  NAALAL L R +       +    + SR ++ G +    V  P                   P + QGL+   WPGR Q L  G   ++++DGAHT  S+Q+ V WF+ QAL         S  R LLF     + +  LL  L             +F  A FCP
Sbjct:   41 EYQDAVRTLNTLQTNANYLEMVKRERGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWSLYHRLEETKD---------------DSSCVS------------MPGYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVPQPDE-PLAVLRD-RAQQISCP---LYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQLARCWLQQKGYQDAGELKASRPSLPGQLPLAPVFQP------------------TPHMQQGLRHTEWPGRTQLLRRG-PLTWYLDGAHTTSSMQACVRWFR-QALQGRGLKDGGSEVRVLLFNSTGDRDSAALLKLLQP----------CQFDYAIFCP 438          

BLAST of EWM25809.1 vs. NCBI_GenBank
Match: gi|803286697|ref|XP_012002996.1| (PREDICTED: folylpolyglutamate synthase, mitochondrial isoform X1 [Ovis aries musimon])

HSP 1 Score: 257.684 bits (657), Expect = 4.035e-74
Identity = 166/462 (35.93%), Postives = 240/462 (51.95%), Query Frame = 0

Query:    2 DMTSQDYITSIAALNSTQLTGDALHRANNSRNSTMEDMHVYLQRLGL-AESLPKLPILHVAGSKGKGSTCAFAEAVLCKQGVRTALFTSPHLVRVNERIRLNGEPLTDKQWSLHFWTVWNRLQDRRSTGLQRSADASLLKSSSSSAASGASTTRIAEPDVTPGYFRLLTLLAFWVAISEQVDVLILEVGVGGRFDATNVVPKPVACAITTLDLEHTDLLGSTIEEIAWEKGGIIKPGTPCFLEPQPTEYQSSILRVLRACVLDAPGAELHVVKPLPG------DVKLGIAGDFQAMNAALALALVREY-------NVYSRRCSRSTIEGSVAEGIVQDPRAESCVAWVDVYPYISTDRPAVSQGLQSCTWPGRCQRLDWGSSCSFFIDGAHTVESIQSAVAWFKGQALSR---------PRALLFYCGEGKTAPELLAHLVTLGSATSSTPYSKFSLAFFCP 440
            DM  QD + ++  L +     + + R      + +E M +YL R GL  E L +L I+H+ G+KGKGSTCAF E +L   G++T  F+SPHLV+V ERIR+NG+P++ + ++ HFW +++RL++ +                 SS  S             PGYFR LTL+AF V + E+VD+ ++EVG+GG +D TN++ KPV C IT+L ++HT LLG+T+E+IAW+KGGI K G P F   QP E   ++LR  RA  +  P   L++  PL         + LG+ G+ Q  NAALAL L R +       +    + SR ++ G +    V  P                   P + QGL+   WPGR Q L  G   ++++DGAHT  S+Q+ V WF+ QAL R          R LLF     + +  LL  L             +F  A FCP
Sbjct:   39 DMEYQDAVRTLNTLQTNANYLEMVKRERGDPQTQLETMKLYLARSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLEETKD---------------DSSCVS------------MPGYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNIIRKPVVCGITSLGIDHTGLLGNTMEKIAWQKGGIFKSGVPAFTVLQPDE-PLAVLRD-RAQQISCP---LYLCPPLQALEEGGPPLTLGLEGEHQRSNAALALQLARCWLQQKGYQDAGELKASRPSLPGQLPPAPVFQP------------------TPHMQQGLRHTEWPGRTQLLRRG-PLTWYLDGAHTTSSMQACVRWFR-QALHRCERPDSGSEVRVLLFNSTGDRDSAALLKVLQP----------CQFDYAIFCP 438