EWM25821.1, cds4819 (CDS) Nannochloropsis gaditana

Overview
NameEWM25821.1
Unique Namecds4819
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002464.1:462790..462846 +
Alignment locationCM002464.1:462925..463191 +
Alignment locationCM002464.1:463326..463625 +

Link to JBrowse

Properties
Property NameValue
Protein idEWM25821.1
Productcytochrome b5 reductase 4
Orig transcript idgnl|cribi|Naga_100021g2.8764.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002464.1supercontigCM002464.1:462790..462846 +
CM002464.1supercontigCM002464.1:462925..463191 +
CM002464.1supercontigCM002464.1:463326..463625 +
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Molecular Function
TermDefinition
GO:0005488binding
GO:0020037heme binding
Vocabulary: INTERPRO
TermDefinition
IPR018506Cyt_B5_heme-BS
IPR001199Cyt_B5-like_heme/steroid-bd
Homology
BLAST of EWM25821.1 vs. NCBI_GenBank
Match: gi|553195200|ref|XP_005856099.1| (hypothetical protein NGA_0331900 [Nannochloropsis gaditana CCMP526] >gi|422292934|gb|EKU20235.1| hypothetical protein NGA_0331900 [Nannochloropsis gaditana CCMP526] >gi|585107688|gb|EWM25821.1| cytochrome b5 reductase 4 [Nannochloropsis gaditana])

HSP 1 Score: 423.705 bits (1088), Expect = 4.094e-150
Identity = 207/207 (100.00%), Postives = 207/207 (100.00%), Query Frame = 0
Query:    1 MATGTPSNKTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYLIEEEGATPKDTGLAQEESPQAGLDGSASSGPAAERAGEDGDSLVTDAAGQSPVDACVGLLAPPPKRFSITAAFGTEEDFAASIEENGAVREG 207
            MATGTPSNKTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYLIEEEGATPKDTGLAQEESPQAGLDGSASSGPAAERAGEDGDSLVTDAAGQSPVDACVGLLAPPPKRFSITAAFGTEEDFAASIEENGAVREG
Sbjct:    1 MATGTPSNKTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYLIEEEGATPKDTGLAQEESPQAGLDGSASSGPAAERAGEDGDSLVTDAAGQSPVDACVGLLAPPPKRFSITAAFGTEEDFAASIEENGAVREG 207          
BLAST of EWM25821.1 vs. NCBI_GenBank
Match: gi|302812502|ref|XP_002987938.1| (hypothetical protein SELMODRAFT_127066 [Selaginella moellendorffii] >gi|302824760|ref|XP_002994020.1| hypothetical protein SELMODRAFT_138045 [Selaginella moellendorffii] >gi|300138123|gb|EFJ04902.1| hypothetical protein SELMODRAFT_138045 [Selaginella moellendorffii] >gi|300144327|gb|EFJ11012.1| hypothetical protein SELMODRAFT_127066 [Selaginella moellendorffii])

HSP 1 Score: 127.102 bits (318), Expect = 3.060e-34
Identity = 57/108 (52.78%), Postives = 74/108 (68.52%), Query Frame = 0
Query:    8 NKTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYL 115
            NK    KGF  MDW+KLT S  DL+G+      R   MEEV +HR  +DAW V  G+VYN+TPYL +HPGG ++++KAAG DCT+LFNK+H WVN E L  + ++G L
Sbjct:    8 NKVPFEKGFSQMDWLKLTQSHPDLAGLKGRSNRRMIAMEEVKQHRKQDDAWTVLRGRVYNITPYLNFHPGGLDMLMKAAGKDCTSLFNKYHAWVNAEFLLERCLLGAL 115          
BLAST of EWM25821.1 vs. NCBI_GenBank
Match: gi|951069380|ref|XP_014490185.1| (PREDICTED: cytochrome b5 domain-containing protein RLF [Vigna radiata var. radiata] >gi|951069382|ref|XP_014490186.1| PREDICTED: cytochrome b5 domain-containing protein RLF [Vigna radiata var. radiata])

HSP 1 Score: 126.331 bits (316), Expect = 1.402e-32
Identity = 53/111 (47.75%), Postives = 73/111 (65.77%), Query Frame = 0
Query:    9 KTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYLIEEE 119
            K    KG+  MDW+KLT +  DL+G+      R   M+EV +H+T  D W V  G+VYN++PY+ +HPGG ++++KA G DCT+LFNK+H WVN E L  K VVG L E +
Sbjct:  116 KVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLISMDEVRKHKTEGDMWTVLKGRVYNISPYMKFHPGGVDMLMKAVGKDCTSLFNKYHAWVNAEFLLEKCVVGTLDESQ 226          
BLAST of EWM25821.1 vs. NCBI_GenBank
Match: gi|1044545041|ref|XP_017438620.1| (PREDICTED: cytochrome b5 domain-containing protein RLF [Vigna angularis] >gi|1044545043|ref|XP_017438621.1| PREDICTED: cytochrome b5 domain-containing protein RLF [Vigna angularis] >gi|1044545045|ref|XP_017438622.1| PREDICTED: cytochrome b5 domain-containing protein RLF [Vigna angularis] >gi|965615837|dbj|BAU00987.1| hypothetical protein VIGAN_11013400 [Vigna angularis var. angularis])

HSP 1 Score: 125.946 bits (315), Expect = 2.007e-32
Identity = 53/111 (47.75%), Postives = 73/111 (65.77%), Query Frame = 0
Query:    9 KTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYLIEEE 119
            K    KG+  MDW+KLT +  DL+G+      R   M+EV +H+T  D W V  G+VYN++PY+ +HPGG ++++KA G DCT+LFNK+H WVN E L  K VVG L E +
Sbjct:  117 KVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLISMDEVRKHKTEGDMWTVLKGRVYNISPYMKFHPGGVDMLMKAVGKDCTSLFNKYHAWVNAEFLLEKCVVGTLDESQ 227          
BLAST of EWM25821.1 vs. NCBI_GenBank
Match: gi|294464394|gb|ADE77709.1| (unknown [Picea sitchensis])

HSP 1 Score: 124.405 bits (311), Expect = 3.500e-32
Identity = 52/107 (48.60%), Postives = 73/107 (68.22%), Query Frame = 0
Query:    9 KTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYL 115
            K    KG+  MDW+KLT +  DL+G+      R   M+EV +H++  D W V  G+VYN++PY+ +HPGG ++++KAAG DCTALFNK+H WVN E L  K +VG+L
Sbjct:   92 KVPFEKGYSQMDWLKLTQTHPDLAGLKGSSNRRLISMDEVKQHKSEGDIWTVLKGRVYNISPYMKFHPGGEDMLMKAAGKDCTALFNKYHAWVNAEFLLEKCLVGFL 198          
BLAST of EWM25821.1 vs. NCBI_GenBank
Match: gi|545371685|ref|XP_005650376.1| (cytochrome b5, partial [Coccomyxa subellipsoidea C-169] >gi|384252356|gb|EIE25832.1| cytochrome b5, partial [Coccomyxa subellipsoidea C-169])

HSP 1 Score: 122.094 bits (305), Expect = 3.621e-32
Identity = 56/107 (52.34%), Postives = 73/107 (68.22%), Query Frame = 0
Query:    9 KTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYL 115
            K  L KG+  MDW++L+  ++DL+G       R   MEEV  HRT +DAW V  GKVYN+TPY+ +HPGGA+ I+K AG DCTALFNK+H WVN + L  K ++G L
Sbjct:    9 KIPLEKGYSQMDWLRLSRGSQDLAGRQGQPLRRDITMEEVKAHRTKSDAWTVLRGKVYNITPYINFHPGGADWIMKGAGMDCTALFNKYHAWVNSDMLLEKCLIGQL 115          
BLAST of EWM25821.1 vs. NCBI_GenBank
Match: gi|633905653|gb|KDD72899.1| (hypothetical protein H632_c2760p0, partial [Helicosporidium sp. ATCC 50920])

HSP 1 Score: 124.02 bits (310), Expect = 4.311e-32
Identity = 58/106 (54.72%), Postives = 72/106 (67.92%), Query Frame = 0
Query:   16 FGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYLIEEEGA 121
             GLMDWV LT S+ DL+G+      R  RMEEV +HRT+ D W V   KVYNLT Y+P+HPGGA I++  AG D TALFNK+HPWVN + L  K +VG L  ++ A
Sbjct:   34 IGLMDWVHLTRSSSDLAGLKGSPPRRDIRMEEVRQHRTATDCWTVLREKVYNLTAYMPFHPGGAKILMACAGRDGTALFNKYHPWVNSDFLLAKCLVGTLHPDDAA 139          
BLAST of EWM25821.1 vs. NCBI_GenBank
Match: gi|1149768040|ref|XP_020148719.1| (cytochrome b5 domain-containing protein RLF [Aegilops tauschii subsp. tauschii])

HSP 1 Score: 124.405 bits (311), Expect = 7.440e-32
Identity = 57/121 (47.11%), Postives = 78/121 (64.46%), Query Frame = 0
Query:    1 MATGTPSNKTALR------KGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYL 115
            +A   P  K+A+R      KGF  MDW+KLT +  DL+G+      R   +EEV +H+T +  W V  G+VYN+ PY+ +HPGG ++++K AG DCTALFNK+H WVN E L  K +VGYL
Sbjct:   98 LAQPKPLKKSAVRAKVPFEKGFSPMDWLKLTRTHPDLAGLKGQSNRRLISLEEVKQHKTGDCVWTVLKGRVYNIGPYMKFHPGGVDMLMKGAGKDCTALFNKYHAWVNAEFLLEKCLVGYL 218          
BLAST of EWM25821.1 vs. NCBI_GenBank
Match: gi|593701193|ref|XP_007151014.1| (hypothetical protein PHAVU_004G011500g [Phaseolus vulgaris] >gi|561024323|gb|ESW23008.1| hypothetical protein PHAVU_004G011500g [Phaseolus vulgaris])

HSP 1 Score: 124.02 bits (310), Expect = 7.501e-32
Identity = 52/111 (46.85%), Postives = 73/111 (65.77%), Query Frame = 0
Query:    9 KTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYLIEEE 119
            K    KG+  MDW+KLT +  DL+G+      R   ++EV +H+T  D W V  G+VYN++PY+ +HPGG ++++KA G DCT+LFNK+H WVN E L  K VVG L E +
Sbjct:  109 KVPFEKGYSQMDWLKLTRTHPDLAGLKGQSNKRLISIDEVRKHKTEGDMWTVLKGRVYNISPYMKFHPGGVDMLMKAVGKDCTSLFNKYHAWVNAEFLLEKCVVGTLDESQ 219          
BLAST of EWM25821.1 vs. NCBI_GenBank
Match: gi|514725904|ref|XP_004955848.1| (PREDICTED: cytochrome b5 domain-containing protein RLF-like [Setaria italica] >gi|944258819|gb|KQL23076.1| hypothetical protein SETIT_031089mg [Setaria italica] >gi|944258820|gb|KQL23077.1| hypothetical protein SETIT_031089mg [Setaria italica])

HSP 1 Score: 124.02 bits (310), Expect = 8.704e-32
Identity = 52/107 (48.60%), Postives = 72/107 (67.29%), Query Frame = 0
Query:    9 KTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGGANIIVKAAGTDCTALFNKHHPWVNLEGLAGKLVVGYL 115
            K    KG+  MDW+KLT +  DL+G+      R   +EEV +H+T +  W V  G+VYN+ PY+ +HPGG ++++KAAG DCTALFNK+H WVN E L  K +VG+L
Sbjct:  104 KVPFEKGYSQMDWLKLTQTHPDLAGLKGQSNRRLISLEEVKQHKTGDCIWTVLKGRVYNIAPYMKFHPGGVDMLMKAAGKDCTALFNKYHAWVNAEFLLEKCLVGFL 210          
The following BLAST results are available for this feature:
BLAST of EWM25821.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|553195200|ref|XP_005856099.1|4.094e-150100.00hypothetical protein NGA_0331900 [Nannochloropsis ... [more]
gi|302812502|ref|XP_002987938.1|3.060e-3452.78hypothetical protein SELMODRAFT_127066 [Selaginell... [more]
gi|951069380|ref|XP_014490185.1|1.402e-3247.75PREDICTED: cytochrome b5 domain-containing protein... [more]
gi|1044545041|ref|XP_017438620.1|2.007e-3247.75PREDICTED: cytochrome b5 domain-containing protein... [more]
gi|294464394|gb|ADE77709.1|3.500e-3248.60unknown [Picea sitchensis][more]
gi|545371685|ref|XP_005650376.1|3.621e-3252.34cytochrome b5, partial [Coccomyxa subellipsoidea C... [more]
gi|633905653|gb|KDD72899.1|4.311e-3254.72hypothetical protein H632_c2760p0, partial [Helico... [more]
gi|1149768040|ref|XP_020148719.1|7.440e-3247.11cytochrome b5 domain-containing protein RLF [Aegil... [more]
gi|593701193|ref|XP_007151014.1|7.501e-3246.85hypothetical protein PHAVU_004G011500g [Phaseolus ... [more]
gi|514725904|ref|XP_004955848.1|8.704e-3248.60PREDICTED: cytochrome b5 domain-containing protein... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna4831rna4831Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications