EWM30200.1, cds476 (CDS) Nannochloropsis gaditana

BLAST of EWM30200.1 vs. NCBI_GenBank
Match: gi|585112858|gb|EWM30200.1| (et-dependent rrna methyltransferase spb1 [Nannochloropsis gaditana])

HSP 1 Score: 2101.63 bits (5444), Expect = 0.000e+0
Identity = 1040/1040 (100.00%), Postives = 1040/1040 (100.00%), Query Frame = 0

Query:    1 MKENLKRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAEMEGGSGDKAISIFSAQYARQKRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPFFEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKEHQGRASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEERLKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLDLLTGRREGDDEESDGTGSDTESGEGEEGEPEEGGEEEEEEEEAGPRRDGGGSGHPLLVDPKVFEGVERNEVKVARWFSNPVLQSVMAGGEEGEEEEEGGERGEDGIGREESGEEEDASHDEESRATGVGKATGGKGAVRRKDVVEEVLAAMPRTDKQLRHAKRMKDRERRAHLAEKKTRAADPDGAVDVEEQAMEVVAAPDPEEEEKRRRRRALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTRGMKKAMKAKKGREVSGEGLEVNILHAQGCSLLSVKCLPGPLFLRFAQLKTKSE 1040
            MKENLKRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAEMEGGSGDKAISIFSAQYARQKRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPFFEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKEHQGRASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEERLKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLDLLTGRREGDDEESDGTGSDTESGEGEEGEPEEGGEEEEEEEEAGPRRDGGGSGHPLLVDPKVFEGVERNEVKVARWFSNPVLQSVMAGGEEGEEEEEGGERGEDGIGREESGEEEDASHDEESRATGVGKATGGKGAVRRKDVVEEVLAAMPRTDKQLRHAKRMKDRERRAHLAEKKTRAADPDGAVDVEEQAMEVVAAPDPEEEEKRRRRRALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTRGMKKAMKAKKGREVSGEGLEVNILHAQGCSLLSVKCLPGPLFLRFAQLKTKSE
Sbjct:    1 MKENLKRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAEMEGGSGDKAISIFSAQYARQKRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPFFEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKEHQGRASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEERLKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLDLLTGRREGDDEESDGTGSDTESGEGEEGEPEEGGEEEEEEEEAGPRRDGGGSGHPLLVDPKVFEGVERNEVKVARWFSNPVLQSVMAGGEEGEEEEEGGERGEDGIGREESGEEEDASHDEESRATGVGKATGGKGAVRRKDVVEEVLAAMPRTDKQLRHAKRMKDRERRAHLAEKKTRAADPDGAVDVEEQAMEVVAAPDPEEEEKRRRRRALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTRGMKKAMKAKKGREVSGEGLEVNILHAQGCSLLSVKCLPGPLFLRFAQLKTKSE 1040          

BLAST of EWM30200.1 vs. NCBI_GenBank
Match: gi|553183202|ref|XP_005853858.1| (et-dependent rrna methyltransferase spb1, partial [Nannochloropsis gaditana CCMP526] >gi|422295206|gb|EKU22505.1| et-dependent rrna methyltransferase spb1, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1080.09 bits (2792), Expect = 0.000e+0
Identity = 531/538 (98.70%), Postives = 532/538 (98.88%), Query Frame = 0

Query:    1 MKENLKRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAEMEGGSGDKAISIFSAQYARQKRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPFFEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKEHQGRASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEERLKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLD 538
            MKENLKRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAEMEGGSGDKAISIFSAQYARQKRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPF EHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKEHQGRASAGTGEEEARAAAAAVLAENTR+RSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEV    GEGGP EEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEERLKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLD
Sbjct:    1 MKENLKRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAEMEGGSGDKAISIFSAQYARQKRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPFLEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKEHQGRASAGTGEEEARAAAAAVLAENTRRRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEV----GEGGP-EEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEERLKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLD 533          

BLAST of EWM30200.1 vs. NCBI_GenBank
Match: gi|298709155|emb|CBJ31099.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 685.641 bits (1768), Expect = 0.000e+0
Identity = 460/1022 (45.01%), Postives = 609/1022 (59.59%), Query Frame = 0

Query:    6 KRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVF---AEMEGGSGDKAISIFSAQYARQ-KRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPFFEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKM----KEHQGRASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEER-LKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLDLLTGRREGDDEESDGTGSDTESGEGEEGEPEEGGEEEEEEEEA-------GPRRDGGGSGHPLLVDPK-----VFEGVERNEVKVARWFSNPVLQSVMAGGEEGEEEEEGGERGEDGIGREESGEEEDASHDEESRATGVGKATGGKGAVRRKDVVEEVLAAMPRTDKQLRHAKRMKDRERRAHLAEKKTRAADPDGAVDVEEQAME---VVAAPDPEEEEKRRRRRALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSL---VPP--------VADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTRGMKKA 992
            KR K  D+DKYY LAK+QG+R+R+AFKLI+IN+KYDFLS AKVC+DLCAAPGGWCQVAAKHMP  SIILGVDLLPIRPI  VKTLVHDITT ECR A+KREM+     DVVLCDGAPNVG  Y KDA+ QNEIAL ALR AT HL  GGTF+TKVYRSQDYN+LMWV  Q F   QA+KP+SSR QSAEIFV+   YKAP  ID ++L+P + F    ++EGG   K +SIF  +Y +  KRHR GY  + G+ L R A V+ F+ +EDPV+ L++   + F   C  F +H  TT E++   +DL+VLGK DF+ L+KWR++M    +E +        E +                + EE+EEE+QAEI+ L+ +  A+ KR RKKERE + R+R R A GM + A DLPD E +FSL +I +  +L+ IRD + +++     +   + EEEE   E+   +       E+++      +LEA L E Y  +L  K+E R ++GTR  KR K   AA A E+  ED+A YDGD + YL LL     G +EE     SD +S +    + +   +  ++   +       G R  G      ++ DP      +    ER +    RWFS+P+   +         EEE  +  +D         +      EE RA   G A    GA       E ++AAMP+TDK+ R  KR K  ER+     K ++    DG  D + Q +     +   DP+E  K  ++R LI+AGMG    + E  G              FEV  A G  +  L     P        V D R Y SD E +DDE+RAR LA+GTMMLR+ +AKE+VDA+YNRF+WND  DLP+WFVDDE+RHYRPQ+P+P  L+++MK +F SLA+KPI KVAEARARK+ R  QRL  AK KA+A+ +NPDM+ER+K+KA+Q+A+K KG K   P KVY V  +G S   K+       A++K+VD RMK DTRG K++
Sbjct:    3 KRTKGQDRDKYYRLAKDQGFRARSAFKLIEINKKYDFLSSAKVCIDLCAAPGGWCQVAAKHMPRGSIILGVDLLPIRPIPNVKTLVHDITTDECRTALKREMQTW-KADVVLCDGAPNVGTAYKKDAYEQNEIALHALRVATQHLKKGGTFVTKVYRSQDYNSLMWVIQQFFEEHQAVKPASSRSQSAEIFVVGRNYKAPDFIDSRMLEPKHAFRQDYDVEGGQ--KGLSIFHKKYDQHNKRHRQGYADDLGMSLSRVAKVSDFVNAEDPVRVLTDTHVLEFGADCQVFKDHRATTAEIQHLCSDLRVLGKGDFRHLIKWRMRMVKYLEELKALEGESGDEGDEEGEGGEGGDAAEVPMTEEEKEEEVQAEIRALQLKALAQRKRVRKKEREAAGRLRSRVAMGMENTAIDLPDEEGVFSLASIKTGGDLESIRDVDLSKLESSAMDVTVDNEEEESGAEDGSSD-------EDDDSDAEDVKLEAHLAEGYLRFLQAKDENRRMEGTRLAKRNKQAKAAKAAEEEMEDMALYDGDQQRYLQLLA---NGGEEEVGIDASDVDSSDDISDDDDSDDDVRDKLSSSVSRAVGRGKRGRGAMGASAVVSDPNPLMAEIGSKSERRQAAAQRWFSDPLFAGI--DDTLEGGEEEEEQEADDKASGVVPPSKRKRGSGEEDRAKRRGAAGEELGAA------EALIAAMPKTDKEKRKEKRKKAAERKERKESKNSKRMGEDGIADDDLQVVSGERDLENLDPKERVKLEKKRGLIRAGMGAAADEAEDTG--------------FEVAKASGAAMAMLGKRYKPKFAVDGLDVEDDRDYGSDQEPYDDEDRARQLALGTMMLRKHRAKEMVDAAYNRFSWNDDADLPDWFVDDEKRHYRPQVPLPPALVEQMKNKFMSLATKPIKKVAEARARKRKRAAQRLTNAKKKATALAANPDMSEREKLKAVQQAMK-KGAKQDIPSKVYVVAGKGPSGTKKKG------AKIKMVDARMKSDTRGAKRS 982          

BLAST of EWM30200.1 vs. NCBI_GenBank
Match: gi|1173932582|gb|OQR84526.1| (rRNA methyltransferase [Achlya hypogyna])

HSP 1 Score: 678.707 bits (1750), Expect = 0.000e+0
Identity = 435/1015 (42.86%), Postives = 600/1015 (59.11%), Query Frame = 0

Query:    5 LKRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAEMEGGSGDKAISIFSAQYARQKRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPFFEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKEHQG---RASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSA-AELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEERLKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLDLLTGRREGDDEESDGTGSDTESGEGEEGEPEEGGEEEEEEEEAGPRRDGGGSGHPLLVDPKVFEGVERNEVKVARWFS-NPVLQSVMAGGEEGEEEEEGGERGEDGIGREESGEEEDASHDEESRATGVGKATGGKGAVRRKDVVEEVLAAMPRTDKQLRHAKRMKDRERRAHLAEKKTRAADPDGA-VDVEEQAMEVVAAPDPEEEEK-------------RRRRRALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTRGMKKAMKAKKGRE 1000
            + ++K+HDKDKYY+LA++QGYR+R+AFKLIQ+N+KYDFLSKAKVC+DLCAAPGGWCQVAAK+MP+ SIILG+DLLPIRPI  VKT   DITT  CR  +++EM++  + DVVLCDGAPNVGA Y KDA+VQNE+AL AL+ A   +G G TF++KV+RSQDYNAL+WVF QLF  V A KP SSR +SAEIFV+C  Y APHSIDPKLLDP +VF+++E    +  ++IF  +Y +QKRHR GYD + G  L R  S++ F++S DP++ L++ +   F+ G   F +H  TT E+K CL+DLKVLGK DFK LLKWR KM+ ++    +A+ G  E     A  A +  N  + + EE+   I+ E+ +LR    AK KRE+KKEREK A+ RLR A GM     +L D    FSLK +G A + L  + DG +A   + +    PE +  ++++++                    A +E+++E+ Y  YL+ K E   K  +A KR K    ALAGE + ED   +DGD   Y  +L                           PEE  +E + EE            +PL+V     +   R    V+RWFS N + + V                                   +++ A  V K               +V+  MP TDK++RH KR +  ER+   A KK R  D + A +  +++ M+  A P+ E+++K             +  + ALIKAGMG  L             +   L + FEVV A  P L     PVAD+RKYDSD+ED+D E+ ART+A+  MML+R  AK+L+D SYNR+AWND  +LP+WFVDDEERHYRPQIP+PK L+ +MKERF  +A+KP+ KVAEARARK+ + ++++K AK KA+ I + PDM+ R+K+KAI KA+K    K  +  K+Y V RRG S+   +      + + K+VD RMKKD R  +K  ++K+GR+
Sbjct:    1 MVKRKKHDKDKYYNLARQQGYRARSAFKLIQLNKKYDFLSKAKVCIDLCAAPGGWCQVAAKYMPASSIILGIDLLPIRPIRGVKTYQCDITTARCRQIIRQEMQSW-NADVVLCDGAPNVGAEYSKDAYVQNELALIALKLAVDVMGKGATFVSKVFRSQDYNALVWVFKQLFKKVSATKPLSSRNESAEIFVVCEDYLAPHSIDPKLLDPEHVFSQLE-KEAEGGLTIFHPKYGQQKRHREGYDDDLGQTLTRVKSISDFVSSTDPIRLLTDCTSFTFAEGDDMFRDHAATTAEIKLCLSDLKVLGKGDFKNLLKWRTKMQTYRDELLKAAKGEEEAAEEEAVVATIQANV-EMTEEEKNAAIREELAQLRANVLAKKKREKKKEREKKAKERLRAAMGMSEAGIELTDDAQAFSLKKLGLAKSNLNDLDDGAYASASDSDELLEPEHDGNDDDDDDM------------------DAVMESQMEKLYDEYLTRKGEG-AKTKKAVKRSKIAKRALAGEALVEDHTMFDGDNEAYNKMLN--------------------------PEESSDESDAEE------------NPLVVS---LKQPTRPSAAVSRWFSGNKLFEDV-----------------------------------KDTPAVTVDKDG-------------DVVPVMPLTDKEIRHKKRKEAAERKERRAAKKQRLEDEELARLASDDEDMD--AGPNDEDDKKGAKAPLSAEQLKRKHEKEALIKAGMGAPL-------------LAAPLSTNFEVVKA-DPEL-----PVADTRKYDSDNEDYDAEDHARTMALAHMMLKRDTAKDLIDNSYNRYAWNDTAELPDWFVDDEERHYRPQIPVPKHLMAQMKERFMEMATKPVKKVAEARARKQRQQLKKVKIAKKKANDIANLPDMSTREKLKAIDKAMKMA--KTKKESKLYVVSRRGRSAANSKSFRKGDKGQTKVVDPRMKKDKRNAEK--RSKRGRK 879          

BLAST of EWM30200.1 vs. NCBI_GenBank
Match: gi|669165780|ref|XP_008618452.1| (methyltransferase [Saprolegnia diclina VS20] >gi|530729785|gb|EQC28027.1| methyltransferase [Saprolegnia diclina VS20])

HSP 1 Score: 667.152 bits (1720), Expect = 0.000e+0
Identity = 434/1001 (43.36%), Postives = 591/1001 (59.04%), Query Frame = 0

Query:    5 LKRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAEMEGGSGDKAISIFSAQYARQKRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPFFEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKEHQG---RASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIG-SAAELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEERLKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLDLLTGRREGDDEESDGTGSDTESGEGEEGEPEEGGEEEEEEEEAGPRRDGGGSGHPLLVDPKVFEGVERNEVKVARWFS-NPVLQSVMAGGEEGEEEEEGGERGEDGIGREESGEEEDASHDEESRATGVGKATGGKGAVRRKDVVEEVLAAMPRTDKQLRHAKRMKDRERRAHLAEKKTRAADPDGAVDVEEQAMEVVAAPDPEEEEKRRRRRALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTRGMKKAMKAKKGRE 1000
            + ++K+HDKDKYY+LA++QGYR+R+AFKLIQ+N+KYDFLSKAKVC+DLCAAPGGWCQVAAK+MP+ SIILG+DLLPIRPI  VKT   DITT  CR  +++EM++  + DVVLCDGAPNVGA Y KDA+VQNE+AL AL+ A   +G G TF++KV+RSQDYNAL+WVF QLF  V A KP SSR +SAEIFV+C  Y APHSIDPKLLDP +VF+ +E    +  ++IF  +Y +QKRHR GYD + G  L R  ++T F+ S DP++ L++ +   F      F EH  TT EVKSCLADLKVLGK DFK+LLKWR KM+ ++    +A+ G  E     A  A +  N  + + EE+   I+ E+ +LR    AK KRE+KKEREK A+ RLR A GM     +L D    FSLK +G   + L  + DG +A   +                 +E  E E G+ G+++++    A +E+++E+ Y  YLS K +   K  +A KR K    ALAGE + ED   +DGD   Y  ++                           PEE  +E + EE            +PL+V  K  + V R    V+RWFS N + + V              +     + ++          D+E R              +RKD  E       R D++    +R++D E    LA   +   + D   D E+           E+ +++  + ALIKAGMG  L             V   L + FEVV A  P L    PP+ D+RK+DSD+ED+D+E+ ART+A+  MMLRR  AK+L+D SYNR+AWNDA DLPEWF DDEERHYRPQIP+PK L+ +MKERF  +A+KP+ KVAEARARK+ + ++++K AK KA+ I + PDM+ R+K+KAI KA+K    K  +  K+Y V RRG ++   +      +   K+VD RMKKD R  +K  +AK+G++
Sbjct:    1 MVKRKKHDKDKYYNLARQQGYRARSAFKLIQLNKKYDFLSKAKVCIDLCAAPGGWCQVAAKYMPASSIILGIDLLPIRPIRGVKTYQCDITTARCRQIIRQEMQSW-NADVVLCDGAPNVGAEYSKDAYVQNELALIALKLAVDVMGKGATFVSKVFRSQDYNALVWVFKQLFKKVSATKPLSSRNESAEIFVVCEDYLAPHSIDPKLLDPEHVFSALE-KDAEGGLTIFHPKYGQQKRHREGYDDDLGQTLTRVKTITEFVESNDPIRLLTDCTSFKFEQADAMFLEHAATTTEVKSCLADLKVLGKGDFKSLLKWRTKMQVYRDELLKAAQGEEEVAEEEAVVATITANV-ELTEEEKNAAIREELAQLRANVLAKKKREKKKEREKKAKERLRAAMGMSEAGIELTDDNQAFSLKKLGLGKSNLNDLDDGAYASASDS----------------DELLEPEHGN-GDDDDDDDMAAIMESQMEKLYDEYLSRKGDG-AKTKKAIKRSKVAKRALAGEALVEDHTMFDGDNEAYEKMIN--------------------------PEESSDESDVEE------------NPLVVSLK--QPV-RPSAAVSRWFSGNKLFEDV-------------KDTPTVVVDKDGDVVPVMPMTDKEIRHK------------KRKDAAE-------RKDRRAAKKQRLEDEE----LARLASDDENMDAGGDDEDDKKGNKTPLTAEQLKRKHEKEALIKAGMGAPL-------------VATPLSTNFEVVKA-DPEL----PPM-DARKFDSDNEDYDEEDHARTMALAHMMLRRDTAKDLIDNSYNRYAWNDAADLPEWFQDDEERHYRPQIPVPKHLMAQMKERFMEMATKPVKKVAEARARKQRQQLKKIKIAKKKANDIANLPDMSTREKLKAIDKAMKMA--KTKKESKLYVVSRRGRTAANSKAFRKGDKGATKVVDPRMKKDKRNTEK--RAKRGKK 880          

BLAST of EWM30200.1 vs. NCBI_GenBank
Match: gi|1210515818|dbj|GAX25341.1| (AdoMet-dependent rRNA methyltransferase SPB1 [Fistulifera solaris])

HSP 1 Score: 568.155 bits (1463), Expect = 0.000e+0
Identity = 420/1050 (40.00%), Postives = 569/1050 (54.19%), Query Frame = 0

Query:    6 KRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKV-CLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAE----MEGG---SGDKAISIFSAQYARQKRHRSGYDGE-AGVLLHRKASVTAFL---ASEDPVQFLSEQSEVVF-------------------SPGCTPFFEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKE----HQGRASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSN-KNEERLKGTRAGKRKKTVTAALAGEKISEDL-------ATYDGDLRGYLDLLTGRREGDDEESDGTGSDTESGEGEEG--EPEEGG--EEEEEEEEAGPRRDGGGSGHPLLVDPKVFEGVERNEVKVARWFSNPVLQSVMAGGEEGEEEEEGGERGEDGIGREESGE-EEDASHDEESRATGVGKATGGKGAVRRKDVV--EEVLAAMPRTDKQLRHAKRMK-------DRERRAHLAEKKTRAAD--PDGAVDVEEQAMEVVAAPDPEEEEKRRRR---RALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKV-AEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTRGMKKA 992
            KRKK HDKDKYY LAKEQG RSRAAFKL QINRKY FL K +   LD+CAAPGGW Q++ K  P    I+ VD+LPIR I  VKTL+ DITT +C+A + + +     VD VL DGAPNVGA Y KDA+ QNE+ L AL+ AT HL PGGTF TKVYRS+DY ++ WV NQLF ++  IKP++SR QSAEIF LCL+YKAP  IDP+L DP ++FA+      GG   S    +++    + +++R R GYD E     +    SV  F+   A +  V  LS+ + + F                   +  C     HPLT+ ++K C++DL+VL +SDFK LL WR +M++       R  A + EE  +        E +     EEE+E++  EI +L+  R  + KR++KKE+E +A+ R R A  +     D  + + IF+L  + S A+L    D    ++ + +  G          E +++E  + G   +E+EE RR A  E  L+++Y  YL N KN E   GTR  KR K +      E+  ED        +  D + R Y ++LTG ++ DD +SD +  D E     +    PEE     ++ + E+  P        +PL+     F   E+  VK ARWFSNP+  S+    EE            D   R  SG  EED S  +E+ A  VG     K   +RK  +  +EVLA +P+TDKQ+RH KR+K        +ERRA    K     +  P G    +E+  +++   D   E +++R    RALIKAGMG      E  G            S  EVVP  G PL     P  D R Y    ED+D ++ A+TLA+GTMMLRRSK K  VDASYNR+AWND  DLP+WFVDDE +HYRPQ+PIP  L+ +MKER  +L +KPI KV      +KK    +     K   +   SN DM+E  K+KAI KA++G+  K  +P K   V ++G       + G  G   VKLVDKRMK D RGMK+A
Sbjct:    5 KRKKFHDKDKYYRLAKEQGLRSRAAFKLSQINRKYPFLEKCQHGVLDICAAPGGWAQMSLKTCPKGIPIVAVDILPIRNIPGVKTLIGDITTAKCQAEIHKTV---GKVDAVLHDGAPNVGAEYGKDAYEQNELVLHALKCATHHLLPGGTFCTKVYRSKDYASIQWVLNQLFGTIHTIKPAASRSQSAEIFFLCLQYKAPTKIDPRLFDPKHIFADDIAASTGGVMASSSSGLTVLDKNWDKKRRKRGGYDMEHLDATMRHIESVRDFVMLPAMKSAVDLLSQSTGLSFYCQSCKEERNAVDKKDNKLTCPCEFLLHHPLTSTDIKECISDLQVLNQSDFKRLLSWRTQMRDALKARDERNEADSDEESDK-------DEKSSDIDSEEEDEKVLQEIAELKARRQRERKRQKKKEKELAAKRRRRAAMDVLQ--IDEAEDDKIFTLATLTSQADLDATVDVNLDQLTDEQIFG---------HESDDDEGNDPGKNADEDEEERRKALREKALDDAYNMYLRNTKNGEAKSGTRMAKRSKKLERQKVLEEALEDQEMALVGKSMSDYNTRTYAEMLTGPKDSDDSDSDASDDDEEDDGFSDDPMTPEEHAILVQQRKSEKKKP-------SNPLI---HSFAEDEKPSVKSARWFSNPLFTSIGKAAEEA-----------DIDSRRRSGRYEEDNSDVDENDADSVG---SDKPKKKRKVGISADEVLAMIPKTDKQVRHEKRLKAMAREERKKERRAKQLGKSEEEFELVPAGEQGSDEKD-DIIDGMDNMSEAQKKRLLEARALIKAGMGAQPGVQEKDG------------SGIEVVP--GQPL-----PKVDLRNYGD--EDYDSDDYAQTLALGTMMLRRSKEKAFVDASYNRYAWNDPEDLPDWFVDDENKHYRPQLPIPPTLVAKMKERVLALTTKPIKKVAEARARKKKRARDKLAAAKKKAEAMAKSNSDMSEAMKLKAISKALRGQDSK--KPGKSVVVAKKG-------RAGMQGAKGVKLVDKRMKADARGMKRA 978          

BLAST of EWM30200.1 vs. NCBI_GenBank
Match: gi|1210524320|dbj|GAX17049.1| (AdoMet-dependent rRNA methyltransferase SPB1 [Fistulifera solaris])

HSP 1 Score: 549.28 bits (1414), Expect = 5.289e-176
Identity = 398/1042 (38.20%), Postives = 553/1042 (53.07%), Query Frame = 0

Query:    6 KRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKV-CLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAE----MEGG---SGDKAISIFSAQYARQKRHRSGYDGE-AGVLLHRKASVTAFL---ASEDPVQFLSEQSEVVF-----------------SPGCTPFF--EHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKEHQGRASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERA-AKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSN-KNEERLKGTRAGKRKKTVTAALAGEKISEDL-------ATYDGDLRGYLDLLTG-RREGDDEESDGTGSDTESGEGEEGEPEEGGEEEEEEEEAGPRRDGGGSGHPLLVDPKVFEGVERNEVKVARWFSNPVLQSVMAGGEEGEEEE--EGGERGEDGIGREESGEEEDASHDEESRATGVGKATGGKGAVRRKDVVEEVLAAMPRTDKQLRHAKRMK-------DRERRAHLAEKKTRAAD--PDGAVDVEEQAMEVVAAPDPEEEEKRR--RRRALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKV-AEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTRGMKKA 992
            KRKK HDKDKYY LAKEQG RSRAAFKL QINRKY FL K +   LD+CAAPGGW Q++ K  P    I+ VD+LPIR I  VKTL+ DITT +C+A + + +     VD VL DGAPNVGA Y KDA+ QNE+ L AL+ AT HL PGGTF TKVYRS+DY ++ WV NQLF ++  IKP++SR QSAEIF LCL+YKAP  IDP+L DP ++FA+      GG   +    +++    + +++R R GYD E     +    SV  F+   A +  V  LS+ + + F                  P C   F   HPLT+ ++K C++DL+VL +SDFK LL WR +M++      A    +E   A +   ++   K S  + EEE +  +Q++ E +A  + +R+R+K++EK    + R+   M     D  + + IF+L  + S A+L    D    ++ + +  G         E ++++      +  E+EEERR+  R E  L+++Y  YL N KN E   GTR  KR K +      E+  ED        +  D + R Y ++LTG +   D +         +    +   PEE     ++ +    +       +PL+     F   E+  VK ARWFSNP+  S+    EE + +    GG   +D    E   +   +   ++ R  G+                +EVLA +P+TDKQ+RH KR+K        ++RRA    K     +  P G    +E+   +    +  E +K+R    RALIKAGMG      E  G            S+ EVVP  G PL     P  D R Y    ED+D ++ A+TLA+GTMMLRRSK K  VDASYNR+AWND  DLP+WFVDDE +HYRPQ+PIP  L+ +MKER  +L  KPI KV      +KK    +     K   +   SN DM+E  K+KAI KA++G+  K  +P K   V ++G       + G  G   VKLVDKRMK D RGMK+A
Sbjct:    5 KRKKFHDKDKYYRLAKEQGLRSRAAFKLSQINRKYPFLEKCQHGVLDICAAPGGWAQMSLKTCPKGIPIVAVDILPIRHIPGVKTLIGDITTAKCQAEIHKTV---GKVDAVLHDGAPNVGAEYGKDAYEQNELVLHALKCATHHLLPGGTFCTKVYRSKDYASIQWVLNQLFGTIHTIKPAASRSQSAEIFFLCLQYKAPTKIDPRLFDPKHIFADDIAASTGGVMATSSSGLTVLDKNWDKKRRKRGGYDMEHLDATMRHIESVRDFVMLPAMKSAVDLLSQSTGLSFYCQTCKEDRNAVDKKGDKPTCPCEFLLHHPLTSADIKECVSDLQVLNQSDFKRLLSWRTQMRD------ALNARDEMNEADSDEESDKDEKSSDIDSEEEDEKVLQEIAELKARRQRERKRQKKKEKELAAKRRRRAAMDVLQIDEAEDDKIFTLATLTSQADLDATVDVNLDQLTDEQIFG--------NESDDDDGNDSGINADEDEEERRKALR-EKALDDAYNMYLRNTKNGEAKSGTRMAKRSKKLERQKVVEEALEDQEMALVGKSMSDYNTRTYAEMLTGPKDSDDSDSDASDDDGDDGFSDDPMTPEEHAILVQQRKSEKTKL-----SNPLI---HSFAEDEKPSVKSARWFSNPLFTSIGKAAEEADTDSRRRGGRDEDDSDADESDADTAGSDKPKKKRKVGIS--------------ADEVLAMIPKTDKQVRHEKRLKAMAREERKKDRRAKQLGKSEEEFELVPAGGQGTDEKDDVIGGMENMSEAQKKRLLEARALIKAGMGAQTGVHEKDG------------SSIEVVP--GQPL-----PKVDLRNYGD--EDYDSDDYAQTLALGTMMLRRSKEKAFVDASYNRYAWNDPEDLPDWFVDDENKHYRPQLPIPPALVAKMKERVLALTMKPIKKVAEARARKKKRARDKLAAAKKKAEAMAKSNSDMSEAMKLKAISKALRGQDSK--KPGKSVVVAKKG-------RAGMQGAKGVKLVDKRMKADARGMKRA 976          

BLAST of EWM30200.1 vs. NCBI_GenBank
Match: gi|553179525|ref|XP_005852714.1| (rrna methyltransferase [Nannochloropsis gaditana CCMP526] >gi|422295818|gb|EKU23117.1| rrna methyltransferase [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 542.732 bits (1397), Expect = 9.729e-176
Identity = 291/293 (99.32%), Postives = 292/293 (99.66%), Query Frame = 0

Query:  695 QLRHAKRMKDRERRAHLAEKKTRAADPDGAVDVEEQAMEVVAAPDPEEEEKRRRRRALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTR 987
            +LRHAKRMKDRERRAHLAEKKTRAADPDGAVDVEEQAMEVVAAPDPEEEEKRRRRRALIKAGMGKVLADGE GGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTR
Sbjct:  485 KLRHAKRMKDRERRAHLAEKKTRAADPDGAVDVEEQAMEVVAAPDPEEEEKRRRRRALIKAGMGKVLADGEGGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTR 777          

BLAST of EWM30200.1 vs. NCBI_GenBank
Match: gi|301123027|ref|XP_002909240.1| (rRNA methyltransferase, putative [Phytophthora infestans T30-4] >gi|262100002|gb|EEY58054.1| rRNA methyltransferase, putative [Phytophthora infestans T30-4])

HSP 1 Score: 493.812 bits (1270), Expect = 6.414e-156
Identity = 263/537 (48.98%), Postives = 365/537 (67.97%), Query Frame = 0

Query:    5 LKRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAEMEGGSGDKAISIFSAQYARQKRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPFFEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKMKEHQGRASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEERLKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLDLLT 541
            + ++K+HDKDKYY+LA++QGYR+R+AFKLIQ+N+KYDFLS AKVC+DLCAAPGGWCQVAAK+MP+ SIILG+DLLPIRPI  VKT   DITT  CR  +K+EM++    DVVLCDGAPNVG  Y KDA+VQNE+AL AL+ A   +G GGTF++KV+RSQDYNAL+WVF QLF  V A KP SSR +SAEIFV+C ++ APHSIDPKL DP YVF +++  S +K I+IF  ++  +KRHR GYD   GV L  + SVT F+ + DP++ L++ + V F P    + +H  T+ E+ +CL+DLKVLGKSDFK+LLKWR +M +++         EE +        E  R+ + +E++  ++ E+ +LR    AK KRE+KKERE+  ++R+R A GM+ D  D+ + ES FSLK      ELK  +    ++V E E  G     +EE + E  +EE E  D G E+ +      LEA +E++Y+ +L+ + ++ +K  +A KR K    ALAGE + +D   +DGD++ Y  ++ 
Sbjct:    1 MVKRKKHDKDKYYNLARQQGYRARSAFKLIQLNKKYDFLSTAKVCIDLCAAPGGWCQVAAKYMPASSIILGIDLLPIRPIRGVKTFQCDITTVRCRQIIKQEMQSW-QADVVLCDGAPNVGTEYSKDAYVQNELALIALKLAVDVMGRGGTFVSKVFRSQDYNALLWVFKQLFKKVSATKPLSSRNESAEIFVVCEQFLAPHSIDPKLFDPKYVFDQVD--SQEKTITIFHPKFGDRKRHREGYDEALGVTLTNECSVTQFIDAHDPIRLLTDTTRVKFLPEDDVYRDHKDTSIEIVTCLSDLKVLGKSDFKSLLKWRTRMLKYKEELLKAKKPEEEKEEEEKPEKEPERELTEKEKDALVREELSQLRANVQAKKKREKKKERERKQKLRIRAALGMNADGIDVTEAESAFSLK------ELKLSK----SKVDELENAGADSSSDEELQFETSDEEDE--DAGLEDSDAEYDELLEASMEKAYEQFLTRRGDD-VKTRKAVKRTKVAKRALAGEALVQDSEMFDGDMKQYQKMIN 521          

HSP 2 Score: 205.297 bits (521), Expect = 5.345e-51
Identity = 136/315 (43.17%), Postives = 194/315 (61.59%), Query Frame = 0

Query:  686 LAAMPRTDKQLRHAKRMKDRERRAHLAEKKTRAA------------DPDGAVDVEEQAMEVVAAPDPEEEEKRRRRRALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTRG 988
            L  MP+TDK++RH KR    ER+    +KK +              D     + +++A     A +  ++E    ++ALI++GMG  L   +   +             FEVV AG  P  + +P + D RKYDSDHE++D E+RA+TLA+ +MM+ +SKAK+++DASYNR+AWND+  LP+WF DDE++HYRPQIPIPK +L +MKERF  +A+KP+ KVAEAR RK+   +++LK AK KA+ I + PDM+ R+K+KAI +A+KG   +  +  KVY V  RGGS  A  K       +VK+VD RMK D R 
Sbjct:  571 LPEMPKTDKEIRHTKRKAAIERKERREKKKLKKEEKEYELEFGTEFDVAAPANSDDEAAMAAEAQEESDDEATAEKKALIRSGMGAALKTDKDSDK-------------FEVVAAGEEPDENALP-IMDDRKYDSDHEEYDAEDRAKTLALASMMIHKSKAKDMIDASYNRYAWNDSEALPDWFADDEDKHYRPQIPIPKNVLAQMKERFMEMATKPVKKVAEARGRKQRMQLKKLKAAKKKATDIANLPDMSTREKLKAIDRAMKGA--RLKKESKVYVVSNRGGSRTADGK-KIGKGKKVKIVDPRMKSDKRN 868          

BLAST of EWM30200.1 vs. NCBI_GenBank
Match: gi|566030000|gb|ETI52911.1| (ribosomal RNA large subunit methyltransferase J [Phytophthora parasitica P1569] >gi|568055735|gb|ETM52476.1| ribosomal RNA large subunit methyltransferase J [Phytophthora parasitica])

HSP 1 Score: 485.723 bits (1249), Expect = 6.942e-153
Identity = 261/538 (48.51%), Postives = 366/538 (68.03%), Query Frame = 0

Query:    5 LKRKKRHDKDKYYHLAKEQGYRSRAAFKLIQINRKYDFLSKAKVCLDLCAAPGGWCQVAAKHMPSDSIILGVDLLPIRPIAKVKTLVHDITTPECRAAVKREMRAHPHVDVVLCDGAPNVGATYDKDAFVQNEIALAALRAATCHLGPGGTFLTKVYRSQDYNALMWVFNQLFASVQAIKPSSSRQQSAEIFVLCLRYKAPHSIDPKLLDPAYVFAEMEGGSGDKAISIFSAQYARQKRHRSGYDGEAGVLLHRKASVTAFLASEDPVQFLSEQSEVVFSPGCTPFFEHPLTTEEVKSCLADLKVLGKSDFKALLKWRLKM-KEHQGRASAGTGEEEARAAAAAVLAENTRKRSPEEEEEEIQAEIQKLREERAAKTKRERKKEREKSARMRLRQAYGMHHDAFDLPDHESIFSLKAIGSAAELKRIRDGEFAEVGEGEGEGGPEEEEEEEEEEEEEEEKEKGDKGEEEEERRRTARLEAELEESYQAYLSNKNEERLKGTRAGKRKKTVTAALAGEKISEDLATYDGDLRGYLDLLT 541
            + ++K+HDKDKYY+LA++QGYR+R+AFKLIQ+N+KYDFLS AKVC+DLCAAPGGWCQVAAK+MP+ SIILG+DLLPIRPI  VKT   DITT  CR  +K+EM++    DVVLCDGAPNVGA Y KDA+VQNE+AL AL+ A   +G GGTF++KV+RSQDYNAL+WVF QLF  V A KP SSR +SAEIFV+C ++ APHSIDPKL DP YVF +++  + +K I+IF  ++  +KRHR GYD   G+ L  + SV+ F+ + DP++ L++ + + F P    + +H  T++E+ +CL DLKVLGK+DFK LLKWR +M K  +    A   EEE +        E  R+ + +E++  ++ E+ +LR    AK KRE+KKERE+  ++R+R A GM+ +  D+ + ES FSLK      ELK  ++    +V E E  G     +EE + E  +EE E  D G E+ +      LEA +E++Y+ +L+ + ++ +K  +A KR K    ALAGE + +D   +DGD++ Y  ++ 
Sbjct:    1 MVKRKKHDKDKYYNLARQQGYRARSAFKLIQLNKKYDFLSTAKVCIDLCAAPGGWCQVAAKYMPASSIILGIDLLPIRPIRGVKTFQCDITTTRCRQIIKQEMQSW-QADVVLCDGAPNVGAEYSKDAYVQNELALVALKLAVDVMGRGGTFVSKVFRSQDYNALLWVFKQLFKKVSATKPLSSRNESAEIFVVCEQFLAPHSIDPKLFDPKYVFDQVD--AQEKTITIFHPKFGDRKRHREGYDEALGMTLTNECSVSQFIDAHDPIRLLTDTTSIKFMPEDDVYRDHKDTSDEIVTCLTDLKVLGKADFKNLLKWRTRMLKYKEELIKAENPEEEEKEEEKPAEKEPERELTEKEKDALVREELSQLRANVQAKKKREKKKERERKQKLRIRAALGMNAEGIDVTEAESAFSLK------ELKMSKN----KVDELENAGADSSSDEELQFETSDEEDE--DAGLEDSDAEYDELLEASMEKAYEQFLTRRGDD-VKTRKAVKRTKVAKRALAGEALVQDSEMFDGDMKQYQKMIN 522          

HSP 2 Score: 213.001 bits (541), Expect = 1.504e-53
Identity = 143/316 (45.25%), Postives = 201/316 (63.61%), Query Frame = 0

Query:  685 VLAAMPRTDKQLRHAKR---MKDRERRAHLAEKKTRAA---------DPDGAVDVEEQAMEVVAAPDPEEEEKRRRRRALIKAGMGKVLADGESGGEDGKRRVEQGLQSTFEVVPAGGPPLLSLVPPVADSRKYDSDHEDWDDEERARTLAIGTMMLRRSKAKELVDASYNRFAWNDAGDLPEWFVDDEERHYRPQIPIPKPLLDEMKERFKSLASKPIAKVAEARARKKHRLVQRLKQAKSKASAIVSNPDMTERDKMKAIQKAVKGKGDKFSRPDKVYAVVRRGGSSQAKQKDGASGRARVKLVDKRMKKDTRG 988
             L  MP+TDK++RHAKR   M+ +ERRA    KK             D     + +++A     A +  ++E    ++ALI++GMG  L            + ++     FEVV AG  P  + +P V D RKYDSDHE++D E+RA+TLA+ +MM+ +SKAK+++DASYNR+AWND+  LP+WF DDEE+HYRPQIPIPK +L +MKERF  +A+KP+ KVAEAR RK+   +++LK AK KA+ I + PDM+ R+K+KAI +A+KG   +  +  KVY V  RGG+  A  K   +G+ +VKLVD RMK D R 
Sbjct:  571 TLPEMPKTDKEIRHAKRKAAMERKERRAMKKLKKEEKEYELEFGTEFDIAAPANSDDEAAMTAEAEEESDDEATADKKALIRSGMGAAL------------KTDKNSSDKFEVVAAGEEPDENALP-VMDERKYDSDHEEYDAEDRAKTLALASMMIHKSKAKDMIDASYNRYAWNDSEALPDWFADDEEKHYRPQIPIPKNVLAQMKERFMEMATKPVKKVAEARGRKQRLQMKKLKAAKKKATDIANLPDMSTREKLKAIDRAMKGA--RLKKESKVYVVSTRGGTKTAGGK--KAGKGKVKLVDPRMKSDKRN 869