EWM26358.1, cds4285 (CDS) Nannochloropsis gaditana | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

Overview


Name	EWM26358.1
Unique Name	cds4285
Type	CDS
Organism	Nannochloropsis gaditana (N. gaditana B-31)
Alignment location	CM002463.1:202886..202954 +
Alignment location	CM002463.1:203206..203868 +

Link to JBrowse

Properties


Property Name	Value
Protein id	EWM26358.1
Product	40s ribosomal protein s3-3
Orig transcript id	gnl\|cribi\|Naga_100031g39.2241.mrna
Gbkey	CDS

Mutants

Expression

No biomaterial libraries express this feature.

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
CM002463.1	supercontig	CM002463.1:202886..202954 +
CM002463.1	supercontig	CM002463.1:203206..203868 +

Analyses

This CDS is derived from or has results from the following analyses


Analysis Name	Date Performed
GO annotation for N. gaditana B31	2020-04-08
BLAST analysis for N. gaditana B-31	2020-04-07
InterPro analysis for N. gaditana B-31	2020-04-06
Gene prediction for N. gaditana B-31	2014-02-18

Annotated Terms

The following terms have been associated with this CDS:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005198	structural molecule activity
GO:0003723	RNA binding
GO:0003735	structural constituent of ribosome

Vocabulary: **Cellular Component**
Term	Definition
GO:0015935	small ribosomal subunit
GO:0005840	ribosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR009019	KH_prok-type
IPR005703	Ribosomal_S3_euk/arc
IPR004044	KH_dom_type_2
IPR001351	Ribosomal_S3_C

Homology

[Close]

BLAST of EWM26358.1 vs. NCBI_GenBank
Match: gi|553193977|ref|XP_005855867.1| (small subunit ribosomal protein S3e [Nannochloropsis gaditana CCMP526] >gi|422293192|gb|EKU20492.1| small subunit ribosomal protein S3e [Nannochloropsis gaditana CCMP526] >gi|585108368|gb|EWM26358.1| 40s ribosomal protein s3-3 [Nannochloropsis gaditana])

HSP 1 Score: 491.886 bits (1265), Expect = 6.872e-176
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query:    1 MTTNISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIELPVPEPVTMSYGGMGGMAPAPMASY 243
            MTTNISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIELPVPEPVTMSYGGMGGMAPAPMASY
Sbjct:    1 MTTNISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIELPVPEPVTMSYGGMGGMAPAPMASY 243

[Close]

BLAST of EWM26358.1 vs. NCBI_GenBank
Match: gi|676390545|ref|XP_009038551.1| (hypothetical protein AURANDRAFT_60177 [Aureococcus anophagefferens] >gi|323450925|gb|EGB06804.1| hypothetical protein AURANDRAFT_60177 [Aureococcus anophagefferens])

HSP 1 Score: 380.948 bits (977), Expect = 3.852e-132
Identity = 183/218 (83.94%), Postives = 200/218 (91.74%), Query Frame = 0

Query:    4 NISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIELPV 221
             ISKKRKF+ADG+FYAELNELLTRELAEDGYAGVEVRV PLRTEIII ATRTQNVLGEKGQRIRELT+V+QKRF+F DG VEL+AE+VA+RGLCAQAQAESLKYKLLGGLAVRRACYGV+RFVME GAKG EV+VSGKLRAQRAK+MKF DGYMIK+G +KREYVD AVRHVLMRQGVLGVKV IMLPHD +GK GPK  L+DVV +LEPKEE  +PV
Sbjct:    3 TISKKRKFVADGLFYAELNELLTRELAEDGYAGVEVRVTPLRTEIIIRATRTQNVLGEKGQRIRELTSVVQKRFHFQDGAVELYAERVANRGLCAQAQAESLKYKLLGGLAVRRACYGVVRFVMESGAKGVEVVVSGKLRAQRAKAMKFTDGYMIKTGHSKREYVDSAVRHVLMRQGVLGVKVSIMLPHDASGKTGPKNTLADVVTILEPKEEAPMPV 220

[Close]

BLAST of EWM26358.1 vs. NCBI_GenBank
Match: gi|325183864|emb|CCA18322.1| (40S ribosomal protein S33 putative [Albugo laibachii Nc14] >gi|325183947|emb|CCA18405.1| 40S ribosomal protein S33 putative [Albugo laibachii Nc14])

HSP 1 Score: 371.318 bits (952), Expect = 4.346e-128
Identity = 181/229 (79.04%), Postives = 202/229 (88.21%), Query Frame = 0

Query:    1 MTTNISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIEL--PVPEPVTM 227
            M TNISKKRKF+ADGVFYAELNELL REL  DGY+GVEVRV PLRTEIII ATRTQ VLGEKGQRIRELT+V+QKRFNF +G VEL+AE++A+RGLC+QAQAESLKY+LL GLAVRRACY VLRFVME GAKG EVIVSGKLRAQRAKSMKF++GYM+K+G A++EYVD AVRHVLMRQGVLGVKV IMLPHDP GK GPK  L DVV +LEPKE+I L  P P+P +M
Sbjct:    1 MATNISKKRKFVADGVFYAELNELLQRELYGDGYSGVEVRVTPLRTEIIIRATRTQEVLGEKGQRIRELTSVVQKRFNFAEGSVELYAERIANRGLCSQAQAESLKYRLLEGLAVRRACYSVLRFVMEAGAKGCEVIVSGKLRAQRAKSMKFKEGYMVKTGNARQEYVDTAVRHVLMRQGVLGVKVSIMLPHDPTGKQGPKRNLDDVVTILEPKEDIPLVYPAPQPQSM 229

[Close]

BLAST of EWM26358.1 vs. NCBI_GenBank
Match: gi|298714483|emb|CBJ27505.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 370.163 bits (949), Expect = 5.621e-128
Identity = 178/215 (82.79%), Postives = 197/215 (91.63%), Query Frame = 0

Query:    5 ISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIEL 219
            +SKKRKF+ADGVFYAELNELLT+ELAE+GYAGVEVRV PLRTEIIISATR QNVLGEKGQRIRELT+V+QKRFNFPD  VEL+AE+VA RGLCAQ QAESLKYKLLGGLAVRRACYGVLRF+ME GAKGAEVIV+GKLRAQRAK+MKF+DGYMIKSG+ + +YVD+AVRHV+MRQGVLGV V IMLPHDP GK GPK  LSDV+ +L PKEEI L
Sbjct:    1 MSKKRKFVADGVFYAELNELLTQELAEEGYAGVEVRVTPLRTEIIISATRPQNVLGEKGQRIRELTSVVQKRFNFPDDAVELYAERVAKRGLCAQTQAESLKYKLLGGLAVRRACYGVLRFIMESGAKGAEVIVAGKLRAQRAKAMKFRDGYMIKSGRVREDYVDHAVRHVMMRQGVLGVTVSIMLPHDPTGKEGPKTPLSDVITILPPKEEIAL 215

[Close]

BLAST of EWM26358.1 vs. NCBI_GenBank
Match: gi|635368781|emb|CCI43086.1| (unnamed protein product [Albugo candida])

HSP 1 Score: 369.777 bits (948), Expect = 2.059e-127
Identity = 181/229 (79.04%), Postives = 202/229 (88.21%), Query Frame = 0

Query:    1 MTTNISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIEL--PVPEPVTM 227
            M TNISKKRKF+ADGVFYAELNELL REL  DGY+GVEVRV PLRTEIII ATRTQ VLGEKGQRIRELT+V+QKRFNF +G VEL+AE++A+RGLC+QAQAESLKY+LL GLAVRRACY VLRFVME GAKG EVIVSGKLRAQRAKSMKF++GYM+K+G A++EYVD AVRHVLMRQGVLGVKV IMLP DP GK GPK  L DVV +LEPKE+I L  PVP+P +M
Sbjct:    1 MATNISKKRKFVADGVFYAELNELLQRELYGDGYSGVEVRVTPLRTEIIIRATRTQEVLGEKGQRIRELTSVVQKRFNFAEGSVELYAERIANRGLCSQAQAESLKYRLLEGLAVRRACYSVLRFVMEAGAKGCEVIVSGKLRAQRAKSMKFKEGYMVKTGNARQEYVDTAVRHVLMRQGVLGVKVSIMLPQDPTGKQGPKRNLDDVVTILEPKEDIPLVFPVPQPQSM 229

[Close]

BLAST of EWM26358.1 vs. NCBI_GenBank
Match: gi|1214672946|gb|OWY96054.1| (40S ribosomal protein S3-3 [Phytophthora megakarya])

HSP 1 Score: 369.007 bits (946), Expect = 3.476e-127
Identity = 177/224 (79.02%), Postives = 196/224 (87.50%), Query Frame = 0

Query:    1 MTTNISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIELPVPEP 224
            M  NISKKRKF+ADGVFYAELNELL REL  DGY+GVEVRV P+RTEIII ATRTQ VLGEKGQRIR LT+V+QKRFNFPDG VEL+AE+VA+RGLC+QAQAESLKY+LLGGLAVRRACY V+RFVME GAKG +VIVSGKLRAQRAK+MKF +GYM+K+G A +EYVD AVRHVLMRQGVLGVKV IMLPHDP GK GPK  L DVV +LEPKEE+  P  EP
Sbjct:    1 MAANISKKRKFVADGVFYAELNELLQRELYGDGYSGVEVRVTPMRTEIIIRATRTQEVLGEKGQRIRALTSVVQKRFNFPDGAVELYAERVANRGLCSQAQAESLKYRLLGGLAVRRACYSVVRFVMEAGAKGVQVIVSGKLRAQRAKAMKFNEGYMVKTGNASQEYVDTAVRHVLMRQGVLGVKVSIMLPHDPTGKQGPKRALDDVVTILEPKEEVHRPYVEP 224

[Close]

BLAST of EWM26358.1 vs. NCBI_GenBank
Match: gi|813164262|ref|XP_012203191.1| (40S ribosomal protein [Saprolegnia parasitica CBS 223.65] >gi|641532155|gb|KDO26198.1| 40S ribosomal protein [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 367.851 bits (943), Expect = 5.995e-127
Identity = 177/221 (80.09%), Postives = 198/221 (89.59%), Query Frame = 0

Query:    4 NISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIELPVPEP 224
            NISKKRKF+ADGVF+AELNELL RELAEDGY+GVEVRV P+RTE+II ATRTQ VLGEKGQRIRELT+V+QKRFNF DG VEL+AE+VA+RGLCAQAQAESLK+KLLGGLAVRRA  GVLRFVME GAKG EVIVSGKLRAQRAK+MKF++GYM+K+G A  EYVD AVRHVLMRQGVLGVKV IMLPHDP G++GPK +L DVV +LEPKEE+  P  EP
Sbjct:    6 NISKKRKFVADGVFFAELNELLQRELAEDGYSGVEVRVTPVRTEVIIRATRTQEVLGEKGQRIRELTSVVQKRFNFADGAVELYAERVANRGLCAQAQAESLKFKLLGGLAVRRAAMGVLRFVMESGAKGCEVIVSGKLRAQRAKAMKFKEGYMVKTGNANLEYVDTAVRHVLMRQGVLGVKVNIMLPHDPTGRMGPKRQLDDVVTILEPKEEVYRPYVEP 226

[Close]

BLAST of EWM26358.1 vs. NCBI_GenBank
Match: gi|301107834|ref|XP_002902999.1| (40S ribosomal protein S3-3 [Phytophthora infestans T30-4] >gi|262098117|gb|EEY56169.1| 40S ribosomal protein S3-3 [Phytophthora infestans T30-4])

HSP 1 Score: 368.237 bits (944), Expect = 6.295e-127
Identity = 177/224 (79.02%), Postives = 196/224 (87.50%), Query Frame = 0

Query:    1 MTTNISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIELPVPEP 224
            M  NISKKRKF+ADGVFYAELNELL REL  DGY+GVEVRV P+RTEIII ATRTQ VLGEKGQRIR LT+V+QKRFNFPDG VEL+AE+VA+RGLC+QAQAESLKY+LLGGLAVRRACY V+RFVME GAKG +VIVSGKLRAQRAK+MKF +GYM+K+G A +EYVD AVRHVLMRQGVLGVKV IMLPHDP GK GPK  L DVV +LEPKEE+  P  EP
Sbjct:    1 MAANISKKRKFVADGVFYAELNELLQRELYGDGYSGVEVRVTPMRTEIIIRATRTQEVLGEKGQRIRALTSVVQKRFNFPDGAVELYAERVANRGLCSQAQAESLKYRLLGGLAVRRACYSVVRFVMEAGAKGVQVIVSGKLRAQRAKAMKFNEGYMVKTGNASQEYVDTAVRHVLMRQGVLGVKVSIMLPHDPTGKQGPKRALDDVVTILEPKEEVHRPYVEP 224

[Close]

BLAST of EWM26358.1 vs. NCBI_GenBank
Match: gi|669149500|ref|XP_008610312.1| (40S ribosomal protein [Saprolegnia diclina VS20] >gi|530737964|gb|EQC36206.1| 40S ribosomal protein [Saprolegnia diclina VS20])

HSP 1 Score: 367.851 bits (943), Expect = 6.930e-127
Identity = 177/221 (80.09%), Postives = 198/221 (89.59%), Query Frame = 0

Query:    4 NISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIELPVPEP 224
            NISKKRKF+ADGVF+AELNELL RELAEDGY+GVEVRV P+RTE+II ATRTQ VLGEKGQRIRELT+V+QKRFNF DG VEL+AE+VA+RGLCAQAQAESLK+KLLGGLAVRRA  GVLRFVME GAKG EVIVSGKLRAQRAK+MKF++GYM+K+G A  EYVD AVRHVLMRQGVLGVKV IMLPHDP G++GPK +L DVV +LEPKEE+  P  EP
Sbjct:    6 NISKKRKFVADGVFFAELNELLQRELAEDGYSGVEVRVTPVRTEVIIRATRTQEVLGEKGQRIRELTSVVQKRFNFADGAVELYAERVANRGLCAQAQAESLKFKLLGGLAVRRAAMGVLRFVMESGAKGCEVIVSGKLRAQRAKAMKFKEGYMVKTGNANLEYVDTAVRHVLMRQGVLGVKVNIMLPHDPTGRMGPKRQLDDVVTILEPKEEVYRPYVEP 226

[Close]

BLAST of EWM26358.1 vs. NCBI_GenBank
Match: gi|953488206|emb|CEG48223.1| (40s ribosomal protein [Plasmopara halstedii])

HSP 1 Score: 368.237 bits (944), Expect = 7.547e-127
Identity = 176/226 (77.88%), Postives = 197/226 (87.17%), Query Frame = 0

Query:    1 MTTNISKKRKFIADGVFYAELNELLTRELAEDGYAGVEVRVVPLRTEIIISATRTQNVLGEKGQRIRELTTVIQKRFNFPDGVVELFAEKVASRGLCAQAQAESLKYKLLGGLAVRRACYGVLRFVMEQGAKGAEVIVSGKLRAQRAKSMKFQDGYMIKSGQAKREYVDYAVRHVLMRQGVLGVKVKIMLPHDPAGKIGPKAKLSDVVNVLEPKEEIELPVPEPVT 226
            M  NISKKRKF+ADGVFYAELNELL REL  DGY+GVEVRV P+RTEIII ATRTQ VLGEKGQRIR LT+V+QKRFNFPDG VEL+AE+VA+RGLC+QAQAESLKY+LLGGLAVRRACY V+RF+ME GAKG +V+VSGKLRAQRAK+MKF +GYM+K+G A +EYVD AVRHVLMRQGVLGVKV IMLPHDP GK GPK  L DVV +LEPKEE+  P  EP T
Sbjct:    1 MAANISKKRKFVADGVFYAELNELLQRELYGDGYSGVEVRVTPMRTEIIIRATRTQEVLGEKGQRIRALTSVVQKRFNFPDGAVELYAERVANRGLCSQAQAESLKYRLLGGLAVRRACYSVVRFIMEAGAKGVQVVVSGKLRAQRAKAMKFNEGYMVKTGNASQEYVDTAVRHVLMRQGVLGVKVSIMLPHDPTGKQGPKRALDDVVTILEPKEEVYRPYVEPTT 226

The following BLAST results are available for this feature:

BLAST of EWM26358.1 vs. NCBI_GenBank

BLAST of EWM26358.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10


Match Name	E-value	Identity	Description
gi\|553193977\|ref\|XP_005855867.1\|	6.872e-176	100.00	small subunit ribosomal protein S3e [Nannochlorops...	[more]
gi\|676390545\|ref\|XP_009038551.1\|	3.852e-132	83.94	hypothetical protein AURANDRAFT_60177 [Aureococcus...	[more]
gi\|325183864\|emb\|CCA18322.1\|	4.346e-128	79.04	40S ribosomal protein S33 putative [Albugo laibach...	[more]
gi\|298714483\|emb\|CBJ27505.1\|	5.621e-128	82.79	conserved unknown protein [Ectocarpus siliculosus]	[more]
gi\|635368781\|emb\|CCI43086.1\|	2.059e-127	79.04	unnamed protein product [Albugo candida]	[more]
gi\|1214672946\|gb\|OWY96054.1\|	3.476e-127	79.02	40S ribosomal protein S3-3 [Phytophthora megakarya...	[more]
gi\|813164262\|ref\|XP_012203191.1\|	5.995e-127	80.09	40S ribosomal protein [Saprolegnia parasitica CBS ...	[more]
gi\|301107834\|ref\|XP_002902999.1\|	6.295e-127	79.02	40S ribosomal protein S3-3 [Phytophthora infestans...	[more]
gi\|669149500\|ref\|XP_008610312.1\|	6.930e-127	80.09	40S ribosomal protein [Saprolegnia diclina VS20] >...	[more]
gi\|953488206\|emb\|CEG48223.1\|	7.547e-127	77.88	40s ribosomal protein [Plasmopara halstedii]	[more]

Relationships

This CDS is a part of the following mRNA feature(s):


Feature Name	Unique Name	Species	Type
rna4295	rna4295	Nannochloropsis gaditana (N. gaditana B-31)	mRNA

Sequences

Synonyms

Publications