EWM26748.1, cds3954 (CDS) Nannochloropsis gaditana

BLAST of EWM26748.1 vs. NCBI_GenBank
Match: gi|585108836|gb|EWM26748.1| (amino acid adenylation protein [Nannochloropsis gaditana])

HSP 1 Score: 1683.69 bits (4359), Expect = 0.000e+0
Identity = 817/817 (100.00%), Postives = 817/817 (100.00%), Query Frame = 0

Query:    1 MPDPAFFQCLGVTRATLTPSLLVNLLHCNAPILSRGQLRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGYYGKQGIHSNFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLRQRLIGRGISPELMPTRIVIGMKDQLLPLTSNGKLDRQMLSTWGSEVASRCTSYAEMVRSRFKDDSSGYWLEFCFSKLVGTQDENHIGLGLFQRGGHSLLAMQALYHIERHFKAASAGVMIEDMARPVAEISKKIGKNRDTLPRKRGRSFTAETISDRNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDLIFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLMPRNSETEIELVLIACQTSLYCIQTSSGKHCWKRKLETRPDEVITGQPTFLEGSLIVLLTTQFGKLLLLNLDSELDAVDTGTRELILTGESRLTSPAIITLPKETVFPYRKSWQCSIGSRDNKVYMINISG 817
            MPDPAFFQCLGVTRATLTPSLLVNLLHCNAPILSRGQLRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGYYGKQGIHSNFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLRQRLIGRGISPELMPTRIVIGMKDQLLPLTSNGKLDRQMLSTWGSEVASRCTSYAEMVRSRFKDDSSGYWLEFCFSKLVGTQDENHIGLGLFQRGGHSLLAMQALYHIERHFKAASAGVMIEDMARPVAEISKKIGKNRDTLPRKRGRSFTAETISDRNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDLIFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLMPRNSETEIELVLIACQTSLYCIQTSSGKHCWKRKLETRPDEVITGQPTFLEGSLIVLLTTQFGKLLLLNLDSELDAVDTGTRELILTGESRLTSPAIITLPKETVFPYRKSWQCSIGSRDNKVYMINISG
Sbjct:    1 MPDPAFFQCLGVTRATLTPSLLVNLLHCNAPILSRGQLRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGYYGKQGIHSNFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLRQRLIGRGISPELMPTRIVIGMKDQLLPLTSNGKLDRQMLSTWGSEVASRCTSYAEMVRSRFKDDSSGYWLEFCFSKLVGTQDENHIGLGLFQRGGHSLLAMQALYHIERHFKAASAGVMIEDMARPVAEISKKIGKNRDTLPRKRGRSFTAETISDRNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDLIFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLMPRNSETEIELVLIACQTSLYCIQTSSGKHCWKRKLETRPDEVITGQPTFLEGSLIVLLTTQFGKLLLLNLDSELDAVDTGTRELILTGESRLTSPAIITLPKETVFPYRKSWQCSIGSRDNKVYMINISG 817          

BLAST of EWM26748.1 vs. NCBI_GenBank
Match: gi|585108835|gb|EWM26747.1| (amino acid adenylation protein [Nannochloropsis gaditana] >gi|585108837|gb|EWM26749.1| amino acid adenylation protein [Nannochloropsis gaditana])

HSP 1 Score: 1681.38 bits (4353), Expect = 0.000e+0
Identity = 817/817 (100.00%), Postives = 817/817 (100.00%), Query Frame = 0

Query:    1 MPDPAFFQCLGVTRATLTPSLLVNLLHCNAPILSRGQLRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGYYGKQGIHSNFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLRQRLIGRGISPELMPTRIVIGMKDQLLPLTSNGKLDRQMLSTWGSEVASRCTSYAEMVRSRFKDDSSGYWLEFCFSKLVGTQDENHIGLGLFQRGGHSLLAMQALYHIERHFKAASAGVMIEDMARPVAEISKKIGKNRDTLPRKRGRSFTAETISDRNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDLIFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLMPRNSETEIELVLIACQTSLYCIQTSSGKHCWKRKLETRPDEVITGQPTFLEGSLIVLLTTQFGKLLLLNLDSELDAVDTGTRELILTGESRLTSPAIITLPKETVFPYRKSWQCSIGSRDNKVYMINISG 817
            MPDPAFFQCLGVTRATLTPSLLVNLLHCNAPILSRGQLRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGYYGKQGIHSNFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLRQRLIGRGISPELMPTRIVIGMKDQLLPLTSNGKLDRQMLSTWGSEVASRCTSYAEMVRSRFKDDSSGYWLEFCFSKLVGTQDENHIGLGLFQRGGHSLLAMQALYHIERHFKAASAGVMIEDMARPVAEISKKIGKNRDTLPRKRGRSFTAETISDRNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDLIFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLMPRNSETEIELVLIACQTSLYCIQTSSGKHCWKRKLETRPDEVITGQPTFLEGSLIVLLTTQFGKLLLLNLDSELDAVDTGTRELILTGESRLTSPAIITLPKETVFPYRKSWQCSIGSRDNKVYMINISG
Sbjct:  259 MPDPAFFQCLGVTRATLTPSLLVNLLHCNAPILSRGQLRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGYYGKQGIHSNFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLRQRLIGRGISPELMPTRIVIGMKDQLLPLTSNGKLDRQMLSTWGSEVASRCTSYAEMVRSRFKDDSSGYWLEFCFSKLVGTQDENHIGLGLFQRGGHSLLAMQALYHIERHFKAASAGVMIEDMARPVAEISKKIGKNRDTLPRKRGRSFTAETISDRNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDLIFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLMPRNSETEIELVLIACQTSLYCIQTSSGKHCWKRKLETRPDEVITGQPTFLEGSLIVLLTTQFGKLLLLNLDSELDAVDTGTRELILTGESRLTSPAIITLPKETVFPYRKSWQCSIGSRDNKVYMINISG 1075          

BLAST of EWM26748.1 vs. NCBI_GenBank
Match: gi|698788056|ref|XP_009827060.1| (hypothetical protein H257_04321 [Aphanomyces astaci] >gi|574118149|gb|ETV83630.1| hypothetical protein H257_04321 [Aphanomyces astaci])

HSP 1 Score: 172.17 bits (435), Expect = 1.732e-40
Identity = 184/702 (26.21%), Postives = 297/702 (42.31%), Query Frame = 0

Query:   12 VTRATLTPSLLVNLLHCNAPILSRGQLRFCLCSGETLS-FITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIV--PLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGYYGKQGIHSNFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKE---ELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLRQRLIGRGISPELMPTRIVIGMKDQLLPLTSNGKLDRQMLSTWGSEVASRCTSYAEMVRSRFKDDSSGYWLEFCF--SKLVGTQDENHIGLGLFQRGGHSLLAMQALYHIERHFKAASA------GVMIEDMARPVAEISKKIGKNRDTLPR----KRGRSFTAETISDR-----NQLPTMLQAWTFEFEKCVDAQPCVVSLDNG-ERLIIVGSHDHYIAALEAN--TGKVKWRSHTSGRIEGKVAVQNDLIFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLM 687
            VTR TL PS+L  LLH  A       ++  + SGETL   +  + L   AP  T+++LYGSTEV GD +  + +       +  +  +V  P+GK +  T++ L+  +          G+     GELWV GPG+ALGY         FV    D    FKTGD      D     L++  R DS  K+ G+ IHL+  E    + L    +     +G   +S +      + ++     A  +        Q  +    +  ++    V+ ++    PLTS GK  R+ L    +++     S  E     +KD  +   +      S      DE    + L + GG+SL+A   L+ +      A+       G  I  +   VA  S  +   +  LP         + T E+   R       L  +  +W+ E EKC+DA P VV+  +     I+VGSHDH++  ++A      + WR+    RIE       + +FV C D  +Y     TG++ W+F T  ++KCTPL +         +K +  V+ GS+D  +Y ++ +SG C  K  P Q   Y++P         +  D+ ++  +  G       +  G V     +       W  ++   VF+  V +T    LV G  DG +  +     GS +WRI      + PIF +PC++
Sbjct:  250 VTRLTLVPSVLQMLLHAAASFPQ--TIQTFVVSGETLRPQLLQQALQAAAPTATVLNLYGSTEVGGDVTCASFSRQSTTSDQIESWGVVGVPIGKAIQHTKLMLMDKD----------GVVSCTRGELWVAGPGLALGYLNPSDTIDKFVHVGSD--LWFKTGDECVWRGDV----LFYSGRVDSQVKIGGISIHLDAVEACFDMYLHQQFQHRPWHVGAVAISMSKPCLQLDTVVVYVGIAGGAATFTSIHSQAALASLFNGHVVVPVRVVVIERTAFPLTSTGKTHRRQLLELYNQINKGNESAPE-----YKDKLTSILMAHLDMPSSTAAIVDE----MTLVELGGNSLVATMVLHDLRTQCGLATLELADVLGKTIGQVRALVALQSIHLHHTKKRLPSLLPGPASPTTTHESRPKRVKTEPTSLGDLYISWSVEVEKCIDATPLVVTHQSAMTTTIVVGSHDHHVTCVDATFPPHAIMWRTKLPDRIESSCVRSENRLFVGCYDGHVYCLHRSTGDVLWSFATNDQIKCTPLVV--------ATKQV--VVCGSHDRHVYGLDIASGKCKFK-LPFQKGVYSTP---------AFQDDMLVCASIGG-------DLRGYVWTSLDIDQPTTPHWMIQLAAPVFSNLV-TTAQKLLVVGCADGCLYGMS-LQTGSTLWRI----ATAKPIFSTPCVL 891          

BLAST of EWM26748.1 vs. NCBI_GenBank
Match: gi|848907581|ref|XP_012852882.1| (PREDICTED: putative acyl-activating enzyme 19 [Erythranthe guttata])

HSP 1 Score: 172.17 bits (435), Expect = 1.860e-40
Identity = 209/878 (23.80%), Postives = 343/878 (39.07%), Query Frame = 0

Query:    6 FFQCLGVTRATLTPSLLVNLL-HCNAPILSRGQ--LRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGYYGKQGIHSNFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLRQRLIGRGISPELM-PTRIVIGMKDQLLPLTSNGKLDRQMLSTWGSEVASRCTSYAEMVRSRFKDDSSGYWLEFCFSKLVGTQDENHIGLGLFQRGGHSLLAMQALYHIERHFK----------------AASAGVMIE---------DMARPVAE--ISKKIGKNRDTL-------------------------PRKRGRSFTAETISDRNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERL-IIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDL--IFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLMPRNSETEIELVLIACQTSLYCIQTSSGKHCWKRKLETRPDEVITGQPTFLEGSLI----VLLTTQFGKLLLLNLDSELDAVDTGTRELILTGESRL-------TSPAIITLPKETVFPYRKSWQCSIGSRDNKVYMI 813
            F Q   + R    PSL+  +L    +    R Q  L+  + SGE L     +TLL   P  ++++LYGSTEV+GD ++       L   K   L  VP+G PL    + LVG +               + GE++V G  VA GY+     +   +         FKTGD  R +S   +     + R+D + KV G  + LEE E         +    +  +V+S   D E   L   +  +       L +   R     + P++M PTRI++    + LPLTS+GK+D   L+   +       +  EM +          + +    + V   D+       F+ GG+S+ A  A + +  H K                ++   V I+         D   P+ +  +  K    RD L                               +  +  I  R+    M   W  + E CVDA P VV    G R+ + +GSH      ++  +G V+W +   GR+E   A+ +D   + V C   ++Y  +   G ++W+F T GE+K  P+            K   LV  GSYD  +Y ++  +  C  K  P  GS + SP +       ++  E +   +  G +  L+++   M  K           W+Q+IG  VF +   +     ++    DG+VV L+P   GS+VW++        PIF  PC    +     E+++ +   S+Y  +T +    WK  + +     IT  P   + S I    + +    G + +L +DS          E  +   +RL       +SP +I              +  +G RDN VY I
Sbjct:  309 FLQGYLINRLVAVPSLIRAILPSLQSRRFRRIQYSLKLLVLSGEVLHLSLCKTLLKLLPQTSVLNLYGSTEVTGDCTYFDCKRLPLILEK-EELSSVPVGLPLSNCEVVLVGEDAP-------------LQGEIYVSGLCVAAGYFD----YPYLIPLADRVQKYFKTGDFARKLS---SGDFVIIGRKDRTLKVNGQRVALEEIETAFR-----DHPSVVDAAVLSREVDGEILLLEAHVVMEEKNEDDELLESSLRNWLLNMLPQVMIPTRILL---TRSLPLTSSGKVDYMSLAASTTSDEQDRINVKEMNQDNLIQVILKVFSDALMVEKVSVDDD------FFEMGGNSISAAYATFKLGIHMKLLYTFPTPRRLQTALLSSKFSVQIDAHSGVDSNMDNYNPIKKLKVESKEQSPRDILWNPGAVVHTECTFSRCNRSTHGVQCEENYSSNAASSKIRPRDGKGFMQVQWKVDMESCVDASPLVVF--KGSRVCVFIGSHSRKFVCIDGKSGVVQWETKLEGRVECSAAILDDFSQVIVGCYKGKIYFLNFSNGSVSWSFQTNGEVKSQPVI----------DKRRHLVWCGSYDHNLYAIDYRNYCCIYK-LPCGGSIFGSPAI-------NEMQENLYVASTSGHITALEIKT--MPFKKL---------WKQDIGAPVFGSLSINYPNGNVICCLVDGSVVVLNP--SGSVVWKV----KTGGPIFAGPC---ASRALPSEVLVCSRDGSIYSFETETSNLIWKHSIGSP----ITSSPYVDDNSPISDRLICVCDSSGSIYVLRVDSNAIGDSEQNTENTVREFARLDLQGDIFSSPVMI------------GGRIFVGCRDNHVYCI 1095          

BLAST of EWM26748.1 vs. NCBI_GenBank
Match: gi|813185937|ref|XP_012207140.1| (hypothetical protein SPRG_21179 [Saprolegnia parasitica CBS 223.65] >gi|641527933|gb|KDO22192.1| hypothetical protein SPRG_21179 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 152.525 bits (384), Expect = 2.446e-34
Identity = 145/549 (26.41%), Postives = 230/549 (41.89%), Query Frame = 0

Query:   38 LRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDAS-WTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGYYGKQGIHSNFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSS-AKVRGVWIHLEEGERELALALGLNS-KEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLRQRLIGRGISPELMPTRI---VIGMKDQLLPLTSNGKLDRQMLSTWGSEVASRCTSYAEMVRSRFKDDSSGYWLEFCFSKLVGTQDENHIGLGLFQRGGHSLLAMQALYHI--ERHFKAASAGVMIEDMARPVAEISKKIGKNRDTLPRKRGRSFTAETISDRNQLPTMLQA--WTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDLIFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPI 576
            LR+ + SGE L       L    P   ++HLYGSTEV+GDA+ W T TT             VP+G  +  T++ LV +E +   E+       +  GEL++ GP +A GY       S+       G   FKTGD    ++  +   L+F  R  +   KV GV +H+   E  +   LG +  K ++ L+ ++S+      + L+           ++  LR  +       E    R+   V+ +     P TS+GK D                     + + F   S   WL     +  G               G + +AM  + H    +H          E   RP+      +   +  + RKR R+ + E    R ++   ++A  W     KC+DA P + + D GE  IIVGSHDH +  + A  G V WR     R+E   AV  + + V C D  +Y   +++G   W F TQ ++KC+P+ +       +E      V+ GS+D  +Y +  +SGAC  K  P   S ++SP+
Sbjct:  222 LRYVVVSGEPLHADLIAALFAALPYVQLLHLYGSTEVAGDATFWLTTTTA----------APVPIGTAIGATKLRLVDAEGA---EIASD----ETRGELYIGGPPLARGYVNPSDNESHRFCMLH-GERWFKTGD----VAYWSKGLLFFCGRTHAGYVKVSGVQVHVAAIETTVRTYLGQHYLKWKVALAPIASSEGWHQYDTLVVCVGMDEDDTMDIDALRDAV-------EREHPRVAMHVLTVPIARFPETSSGKAD------------------PSQIPALFAQRSLPSWLRALLPRTSGLASTRLDPSTTLTELGLNSMAMAIVLHTLQSQH----------EMTLRPIELADMTVADLQALVQRKRPRAISPEASRKRTRVAPRMEAVRWRVRAHKCIDASPRLATTDVGE-YIIVGSHDHTVLCVAAVDGHVVWRKELPDRVESSAAVAKNQVVVGCYDGGVYCLDVLSGSTLWVFFTQDQVKCSPVIV-------EEENA---VVVGSHDHHVYGLALTSGACLFK-LPFTKSVFSSPV 701          

BLAST of EWM26748.1 vs. NCBI_GenBank
Match: gi|1072992775|ref|XP_018447190.1| (PREDICTED: putative acyl-activating enzyme 19 isoform X2 [Raphanus sativus])

HSP 1 Score: 132.109 bits (331), Expect = 8.701e-28
Identity = 89/318 (27.99%), Postives = 146/318 (45.91%), Query Frame = 0

Query:  415 RNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDL--IFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRI-------LLDQVQSSPIFISPCLMPRNSETEIELVLIACQTS-LYCIQTSSGKHCWKRKL 722
            RNQ+ ++ + W    E CVDA P VV L + +  + +GSH      ++A +G + W +   GRIEG   V  D   + + C   +LY    +TG L+WTF   GE+KC P+        H  S+   L+  GS+D  +Y ++  S  C  K     GS +ASP++  G          +   +  GRV  + ++               H+ WE E+   +F +     ++  ++    DG VVA+ P   G+I+W++       L+    S PIF  PC+    S      VL+ C+   +Y +++ SG H W+  +
Sbjct:  674 RNQIVSIQELWKVHMESCVDASPLVV-LKHSKTYLFIGSHSRKFVCIDAKSGSICWETILEGRIEGSAMVVGDFSQVVIGCYKGKLYFLDFLTGSLSWTFQAGGEIKCQPV-------VHTSSQ---LIWCGSHDHTLYALDYRSQRCVYK-LQCGGSIFASPVIDEGH-------SSLYVASTSGRVTAVSIK-----------DLPFHTLWELELEAPIFGSLSIIPSSRNVICCLVDGQVVAMSP--SGTIIWKLSNYSVITLMQYRTSGPIFAGPCM----SHVLPSQVLVCCRNGRVYSLESESGCHLWEYNI 955          

HSP 2 Score: 66.2402 bits (160), Expect = 1.885e-7
Identity = 82/314 (26.11%), Postives = 134/314 (42.68%), Query Frame = 0

Query:    5 AFFQCLGVTRATLTPSLLVNLLHCNAPILS-RGQ------LRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGY---------YGKQGIHS--NFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLR--QRLIGRGISPELMPTRIVIGMKDQLLPLTSNGKLDRQMLS 298
             F +   ++R    PS++  +L    P L  RG       L+  + SGE        +L    P    ++LYGSTEVSGD ++    +G  +  +T N+  VP+GKP+   ++ L+G E                 GE+ V G  ++ GY         Y K   +S  N +  +      ++TGD  R +S   +  L F+ RRD + K+ G  + LEE E  L L         +  +VV  N D      L A      ++ P+   +   +  I   +   ++P   V+    + LP TS+GK+D + L+
Sbjct:  190 GFLEAYSISRLVAVPSMIRAIL----PTLQHRGHNKLKSCLKLVVLSGEPFPVSMWDSLHKLLPETCFLNLYGSTEVSGDCTYFD-CSGLPKLLETENIGSVPIGKPISNCKILLLGDEDKPS------------EGEICVGGLCLSQGYLHSSVDSQSYVKLHNNSLCNHLTVDCGSQLYYRTGDYGRRLS---SGDLIFIGRRDRTVKLNGQRMALEEIETTLVL------NPYITEAVVIYNRDQTELASLDAFLVLNKEAKPDEDVIYSIRNWISDKLPSVMIPNHFVLV---ESLPSTSSGKVDYEALA 474          

BLAST of EWM26748.1 vs. NCBI_GenBank
Match: gi|1072992773|ref|XP_018447189.1| (PREDICTED: putative acyl-activating enzyme 19 isoform X1 [Raphanus sativus])

HSP 1 Score: 130.568 bits (327), Expect = 3.107e-27
Identity = 88/311 (28.30%), Postives = 143/311 (45.98%), Query Frame = 0

Query:  415 RNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDL--IFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLMPRNSETEIELVLIACQTS-LYCIQTSSGKHCWKRKL 722
            RNQ+ ++ + W    E CVDA P VV L + +  + +GSH      ++A +G + W +   GRIEG   V  D   + + C   +LY    +TG L+WTF   GE+KC P+        H  S+   L+  GS+D  +Y ++  S  C  K     GS +ASP++  G          +   +  GRV  + ++               H+ WE E+   +F +     ++  ++    DG VVA+ P   G+I+W+       S PIF  PC+    S      VL+ C+   +Y +++ SG H W+  +
Sbjct:  792 RNQIVSIQELWKVHMESCVDASPLVV-LKHSKTYLFIGSHSRKFVCIDAKSGSICWETILEGRIEGSAMVVGDFSQVVIGCYKGKLYFLDFLTGSLSWTFQAGGEIKCQPV-------VHTSSQ---LIWCGSHDHTLYALDYRSQRCVYK-LQCGGSIFASPVIDEGHS-------SLYVASTSGRVTAVSIK-----------DLPFHTLWELELEAPIFGSLSIIPSSRNVICCLVDGQVVAMSP--SGTIIWKYR----TSGPIFAGPCM----SHVLPSQVLVCCRNGRVYSLESESGCHLWEYNI 1062          

HSP 2 Score: 66.6254 bits (161), Expect = 1.770e-7
Identity = 82/314 (26.11%), Postives = 134/314 (42.68%), Query Frame = 0

Query:    5 AFFQCLGVTRATLTPSLLVNLLHCNAPILS-RGQ------LRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGY---------YGKQGIHS--NFVAKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLR--QRLIGRGISPELMPTRIVIGMKDQLLPLTSNGKLDRQMLS 298
             F +   ++R    PS++  +L    P L  RG       L+  + SGE        +L    P    ++LYGSTEVSGD ++    +G  +  +T N+  VP+GKP+   ++ L+G E                 GE+ V G  ++ GY         Y K   +S  N +  +      ++TGD  R +S   +  L F+ RRD + K+ G  + LEE E  L L         +  +VV  N D      L A      ++ P+   +   +  I   +   ++P   V+    + LP TS+GK+D + L+
Sbjct:  308 GFLEAYSISRLVAVPSMIRAIL----PTLQHRGHNKLKSCLKLVVLSGEPFPVSMWDSLHKLLPETCFLNLYGSTEVSGDCTYFD-CSGLPKLLETENIGSVPIGKPISNCKILLLGDEDKPS------------EGEICVGGLCLSQGYLHSSVDSQSYVKLHNNSLCNHLTVDCGSQLYYRTGDYGRRLS---SGDLIFIGRRDRTVKLNGQRMALEEIETTLVL------NPYITEAVVIYNRDQTELASLDAFLVLNKEAKPDEDVIYSIRNWISDKLPSVMIPNHFVLV---ESLPSTSSGKVDYEALA 592          

BLAST of EWM26748.1 vs. NCBI_GenBank
Match: gi|633267002|gb|AHZ46177.1| (NRPS [uncultured bacterium 14-4D])

HSP 1 Score: 129.798 bits (325), Expect = 5.160e-27
Identity = 116/364 (31.87%), Postives = 171/364 (46.98%), Query Frame = 0

Query:   12 VTRATLTPSLLVNLLHCNAPILSR-GQLRFCLCSGETLSFITARTLLMRAPLCTIIHLYGSTEVSGDASWTTLTTGDLEYGKTANLDIVPLGKPLPGTRMWLVGSETSTKLEVGQIGMPVDMNGELWVEGPGVALGYYGKQGIHSN-FV----AKERDGHPVFKTGDLVRCISDTTNTSLYFLHRRDSSAKVRGVWIHLEEGERELALALGLNSKEELGLSVVSSNTDTENSELLIALTWKAAQSWPNLPKLRQRLIGRGISPELMPTRIVIGMKDQLLPLTSNGKLDRQMLSTWGSEVASRCTSYAEMVRSRFKDDSSGYWLEFCFSKLVGTQDENHIGLGLFQRGGHSLLAMQALYHIERHF 369
            VTR  L PSLL  LL     + +R  +LR  +CSGETL     R      P   +++LYGS+EV+GD   T   T +L+ G  A    VP+G+P+  TR+++  +        GQ  MP+ + GEL V G GVA GY+ +  + +  F+    + E D   +++ GDL R     T+ SL +L R+D   K+RG  + L + E  LA      S +E  +++    TD      L+A     A   P+  +LRQ L  +   P  M     + +  +  PLT +GK+DRQ L     +   R  SY    RS  ++     W E      +G  D+       F  GGHSLLA + +  I   F
Sbjct:  736 VTRIVLVPSLLSALLDAENDLAARLPRLRLWVCSGETLPVSMCRRFQEAVPGAVLLNLYGSSEVAGDV--TCFNTEELDEGVGA----VPIGRPIANTRIYVCDT-------AGQ-PMPIGIAGELCVGGDGVANGYWCQAELTAERFIEDPYSAESDAR-LYRMGDLARW---RTDGSLDYLGRKDRQVKIRGFRVELGDVEAMLA---NHPSVDECAVALWGKKTDERG---LVAYVVPRAYPGPSAHELRQYLEAK--LPHYMVPFAFVAL--EAFPLTPSGKIDRQNLPPPDGQHIEREQSYV-APRSSIEEQIVAIWAELLVRDPIGVYDD------FFSLGGHSLLAARVMNRITERF 1064          

BLAST of EWM26748.1 vs. NCBI_GenBank
Match: gi|1079434112|ref|XP_018509516.1| (PREDICTED: putative acyl-activating enzyme 19 isoform X5 [Brassica rapa])

HSP 1 Score: 128.642 bits (322), Expect = 7.401e-27
Identity = 89/311 (28.62%), Postives = 141/311 (45.34%), Query Frame = 0

Query:  415 RNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDL--IFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLMPRNSETEIELVLIACQT-SLYCIQTSSGKHCWKRKL 722
            RNQ+ ++ + W    E CVDA P VV L + +  + +GSH      ++AN+G + W +   GRIEG   V  D   + + C   +LY     TG L WTF   GE+KC P+        H  S+   L+  GS+D  +Y ++  S  C  K     GS +ASPI+  G          +   +  GRV  + ++            +  H+ W  E+   +F +     ++  ++    DG VVA+ P   G+I+W+         PIF  PC+    S      VL+ C+  S+Y ++  SG H W+  +
Sbjct:  530 RNQMVSIQELWKVHMESCVDASPLVV-LKHSKTYLFIGSHSRKFFCIDANSGSICWETILEGRIEGSAMVVGDFSQVVIGCYKEKLYFLDFSTGSLCWTFQAGGEIKCQPV-------VHTSSQ---LIWCGSHDHTLYALDYRSQRCVYK-LQCGGSIFASPIIDEGHS-------SLYVASTSGRVTAVSIK-----------DFPFHTLWVLELDAPIFGSLSIIPSSRSVICCLVDGQVVAISP--SGTIIWKYR----TGGPIFAGPCM----SYVLPSQVLVCCRNGSVYSLEPESGCHLWEYNI 800          

BLAST of EWM26748.1 vs. NCBI_GenBank
Match: gi|685343091|ref|XP_009108010.1| (PREDICTED: putative acyl-activating enzyme 19 isoform X1 [Brassica rapa])

HSP 1 Score: 128.642 bits (322), Expect = 1.042e-26
Identity = 89/311 (28.62%), Postives = 141/311 (45.34%), Query Frame = 0

Query:  415 RNQLPTMLQAWTFEFEKCVDAQPCVVSLDNGERLIIVGSHDHYIAALEANTGKVKWRSHTSGRIEGKVAVQNDL--IFVPCLDYRLYAFSLVTGELAWTFITQGELKCTPLPLWLPSPTHDESKGISLVLFGSYDGRIYVVEASSGACFAKPFPLQGSPYASPIVIPGEKRNSDSDERIITVTNRGRVAQLKLEREGMVLKGAHLRWALHSDWEQEIGCAVFTTPVYSTTTAYLVFGGTDGAVVALDPFHGGSIVWRILLDQVQSSPIFISPCLMPRNSETEIELVLIACQT-SLYCIQTSSGKHCWKRKL 722
            RNQ+ ++ + W    E CVDA P VV L + +  + +GSH      ++AN+G + W +   GRIEG   V  D   + + C   +LY     TG L WTF   GE+KC P+        H  S+   L+  GS+D  +Y ++  S  C  K     GS +ASPI+  G          +   +  GRV  + ++            +  H+ W  E+   +F +     ++  ++    DG VVA+ P   G+I+W+         PIF  PC+    S      VL+ C+  S+Y ++  SG H W+  +
Sbjct:  766 RNQMVSIQELWKVHMESCVDASPLVV-LKHSKTYLFIGSHSRKFFCIDANSGSICWETILEGRIEGSAMVVGDFSQVVIGCYKEKLYFLDFSTGSLCWTFQAGGEIKCQPV-------VHTSSQ---LIWCGSHDHTLYALDYRSQRCVYK-LQCGGSIFASPIIDEGHS-------SLYVASTSGRVTAVSIK-----------DFPFHTLWVLELDAPIFGSLSIIPSSRSVICCLVDGQVVAISP--SGTIIWKYR----TGGPIFAGPCM----SYVLPSQVLVCCRNGSVYSLEPESGCHLWEYNI 1036