EWM26809.1, cds3851 (CDS) Nannochloropsis gaditana

BLAST of EWM26809.1 vs. NCBI_GenBank
Match: gi|585108900|gb|EWM26809.1| (autophagy-related 7 isoform cra_a [Nannochloropsis gaditana])

HSP 1 Score: 1404.81 bits (3635), Expect = 0.000e+0
Identity = 695/695 (100.00%), Postives = 695/695 (100.00%), Query Frame = 0

Query:    1 MLRGHQTECFHLLVRLSHGGQCPALPALEGARPFGRGWARGRKPIFFRDRVLSSVPGLAGVPSTSLPGDGRRVLSSLLPHPPFHDPPPQTADDREGGSSSLSLEVCPLDAYEKMDRSHGEQPQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWGLCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMHKGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVSSPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTAGESKARTGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEASGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCNEQGVLEEMTGLAQMRRETDALELSWSEDEDEEEEEELGGGGKEKEVEMGEKEQKGREKPTQEWDVKEEEKKSGGLDLALEDLAIEHAGIRRTGGNCE 695
            MLRGHQTECFHLLVRLSHGGQCPALPALEGARPFGRGWARGRKPIFFRDRVLSSVPGLAGVPSTSLPGDGRRVLSSLLPHPPFHDPPPQTADDREGGSSSLSLEVCPLDAYEKMDRSHGEQPQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWGLCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMHKGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVSSPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTAGESKARTGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEASGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCNEQGVLEEMTGLAQMRRETDALELSWSEDEDEEEEEELGGGGKEKEVEMGEKEQKGREKPTQEWDVKEEEKKSGGLDLALEDLAIEHAGIRRTGGNCE
Sbjct:    1 MLRGHQTECFHLLVRLSHGGQCPALPALEGARPFGRGWARGRKPIFFRDRVLSSVPGLAGVPSTSLPGDGRRVLSSLLPHPPFHDPPPQTADDREGGSSSLSLEVCPLDAYEKMDRSHGEQPQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWGLCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMHKGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVSSPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTAGESKARTGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEASGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCNEQGVLEEMTGLAQMRRETDALELSWSEDEDEEEEEELGGGGKEKEVEMGEKEQKGREKPTQEWDVKEEEKKSGGLDLALEDLAIEHAGIRRTGGNCE 695          

BLAST of EWM26809.1 vs. NCBI_GenBank
Match: gi|553185650|ref|XP_005854285.1| (autophagy-related protein 7 [Nannochloropsis gaditana CCMP526] >gi|422294776|gb|EKU22076.1| autophagy-related protein 7 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1173.69 bits (3035), Expect = 0.000e+0
Identity = 582/593 (98.15%), Postives = 585/593 (98.65%), Query Frame = 0

Query:  105 VCPLDAYEKMDRSHGEQPQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWGLCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMHKGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVSSPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTAGESKAR--TGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEASGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCNEQGVLEEMTGLAQMRRETDALELSWSEDEDEEEEEELGGGGKEKEVEMGEKEQKGREKPTQEWDVKEEEKKSGGLDLALEDLAIEHAGIRRTGGNCE 695
            VCPLDAYEKMDR HGEQ QVMFGYVDPGTVPDAPGWPLRNFLLLLANRWGLCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGAL TTGWEMHKGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAA ALRRIFPGVVAEGHVMTIPMPGHPVSSPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTA ESKAR  TGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEASGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLV+RVCNEQGVLEEMTGLAQMRRETDALELSWSEDEDEEEEEELGGGGKEKEVEMGEKEQKGREKPTQEW+VKEEEK SGGLDLALE+LAIEHAGIRRTGGNCE
Sbjct:  113 VCPLDAYEKMDRFHGEQQQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWGLCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALMTTGWEMHKGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAATALRRIFPGVVAEGHVMTIPMPGHPVSSPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTARESKARTGTGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEASGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVLRVCNEQGVLEEMTGLAQMRRETDALELSWSEDEDEEEEEELGGGGKEKEVEMGEKEQKGREKPTQEWNVKEEEKNSGGLDLALEELAIEHAGIRRTGGNCE 705          

BLAST of EWM26809.1 vs. NCBI_GenBank
Match: gi|1191052561|ref|XP_020904175.1| (ubiquitin-like modifier-activating enzyme ATG7 [Exaiptasia pallida] >gi|999973030|gb|KXJ12204.1| Ubiquitin-like modifier-activating enzyme ATG7 [Exaiptasia pallida])

HSP 1 Score: 484.182 bits (1245), Expect = 3.730e-159
Identity = 251/530 (47.36%), Postives = 337/530 (63.58%), Query Frame = 0

Query:  108 LDAYEKMDRSHGEQPQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWG--LCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMHKGKYM-PRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVSSPAEEASA-RREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTAGESKARTGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEASGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCNEQGVLEEMTGLAQMRRETDALELSWSEDEDEEEEE 633
            L + +++D+    + Q+M G+ DP T+P  PGW LRNFL  LA  WG  L    ++C+R R  G                  G + +  S V+HVS+  L +      P  +   GWE +K + + PR VDLSATMDP +LAE SVDLNLKLMRWRLLP+LD+  +++TKCLLLGAGTLGC+VAR LLGWGVR ITLVDNGR++ SNPVRQ+LFE+ DC   GKPKA  AA ALR+IFPGV A G + +IPMPGH + S  E   A +R+V+ L+ L+  HDV++LL DTRESRWLPT++ A  +K+++ +ALGFDTF+VMRHGA+                     G+          G   G  +GCYFCNDVVAP +ST+DRTLDQQCTV+RPG++ +ASALAVELMV++L HP + +A A+    S        D + PLG++P QIRG+L+ ++T+LP + AF  C  C+  VI +Y  EG   +++  N    LE++TGL ++  +T   E+ W   +DE  EE
Sbjct:  203 LSSLKEIDQFIETEQQLMVGFSDPSTMPSNPGWQLRNFLYFLAYHWGDKLTTVKILCFRDRFRG------------------GKREINHSIVIHVSVPALNNKEF---PKCI---GWEKNKNQKLGPRLVDLSATMDPEKLAESSVDLNLKLMRWRLLPSLDLDVISQTKCLLLGAGTLGCNVARCLLGWGVRNITLVDNGRISYSNPVRQTLFEFEDCKGSGKPKAETAANALRKIFPGVNACGEMFSIPMPGHAIGSSKEAQDAVQRDVQRLEALIDAHDVVYLLMDTRESRWLPTLIAAAKEKLVMTSALGFDTFLVMRHGARK-------------------VGQQPIDISSSGLGAIPGAYLGCYFCNDVVAPGNSTRDRTLDQQCTVSRPGMSFIASALAVELMVSVLQHPNKGQAXAN---TSAKDSHLTADFETPLGLVPHQIRGFLSRYSTVLPASLAFDKCIACSDVVISSYMNEGFSFLLKAFNLPKYLEDLTGLTKLMNDTRVEEV-WEMSDDESLEE 685          

BLAST of EWM26809.1 vs. NCBI_GenBank
Match: gi|1183492393|gb|ORZ16069.1| (E1-like protein-activating [Lobosporangium transversale])

HSP 1 Score: 482.641 bits (1241), Expect = 2.729e-158
Identity = 251/513 (48.93%), Postives = 329/513 (64.13%), Query Frame = 0

Query:  123 QVMFGYVDPGTVPDAPGWPLRNFLLLLANRWGLCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHV---SLAPLASSPSSSPPGALTTTGWEMH-KGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVSSPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTAGESKARTGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEASGLGH-RFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCNEQGVLEEMTGLAQMRRETDALELSWSEDEDEE 630
            Q++ G+ DP ++   PGWPLRNFL LL  +W + +  V+C+R                     + G Q +  S +  V   S  PL++ P +         GWE + +GK  PR  DL+  MDP RLA+ +VDLNLKLMRWRLLP L +  +  T+CLLLGAGTLGC VAR LLGWG+R ITLVDN +V+ SNPVRQ LFE+ DC +GGK KA+ AAA+LR+++PGV A GH ++IPMPGHP+ SP    S +R+VE L+ L  +HDVIFLL D+RESRWLPT+LGA   KI++NAALGFDTF+VMRHG                    G   E  A  G  SP       +GCYFCNDVVAP DS  DRTLDQQCTVTRPGL+ +ASA+AVE++V+LLHHP   RAPAD  +  G    R  ++    LG+LP QIRG+L+ F  +L   +A+  CT C+ A++EAY++EG G + +V  +   LE++TGL QM+ +++ L + W ED DE+
Sbjct:  238 QIVVGFADPSSLDSNPGWPLRNFLTLLYYQWRVSKVRVLCFR--------------------EVPGKQDISASKIFTVEVPSYVPLSTCPKA--------VGWEKNAQGKLGPRLADLAPLMDPTRLADTAVDLNLKLMRWRLLPDLQLEKVQETRCLLLGAGTLGCYVARSLLGWGIRNITLVDNAKVSFSNPVRQPLFEFEDCLEGGKDKAITAAASLRKVYPGVNAVGHTLSIPMPGHPI-SPQLYESVQRDVEKLEDLFQQHDVIFLLMDSRESRWLPTLLGARMKKIVINAALGFDTFLVMRHGI-------------------GQDHEYHASFG-SSP-------LGCYFCNDVVAPTDSLSDRTLDQQCTVTRPGLSAIASAVAVEMLVSLLHHPDGMRAPADKADDEGDQEGRTQNNQVSTLGLLPHQIRGFLSRFKNLLISGQAYDRCTACSDAILEAYEKEGFGFLAKVFQDAKHLEDVTGLTQMKEQSEELNMEWDEDSDED 694          

BLAST of EWM26809.1 vs. NCBI_GenBank
Match: gi|1025081225|ref|XP_016340089.1| (PREDICTED: ubiquitin-like modifier-activating enzyme ATG7 isoform X2 [Sinocyclocheilus anshuiensis])

HSP 1 Score: 481.871 bits (1239), Expect = 6.816e-158
Identity = 264/539 (48.98%), Postives = 345/539 (64.01%), Query Frame = 0

Query:  102 SLEVCPLDAYEKMDRSHGEQPQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWG--LCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMH-KGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVS-SPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTAGESKARTGKGSPGPA-----DGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEA-SGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCN-EQGVLEEMTGLAQMRRETDALELSWSEDEDE 629
            S+EV PL   + ++    +  ++  G  DP T+P  PGWPLRNFL+LLA +WG  L    V+C+R R                   L G++ +R S +  V L  L +S          + GWE + KG   PR V+LS  MDP RLAE SVDLNLKLMRWRL+P+LD+  +  T+CLLLGAGTLGC+VAR L+GWGVR IT VDN +++ SNPVRQ L+E+ DC  GGK KALAA   L++IFPGV AEG+ M+IPMPGHPV+ S    A AR++VE L+KL++EHDV+FLL DTRESRWLPTV+ A   K++VNAALGFDTF+VMRHG +   P  S +   S       +  S       +PG +      G  +GCYFCNDVVAP DST+DRTLDQQCTV+RPGLA +A ALAVELMV++L HP       +GG A +      +++P   LG++P QIRG+L+ F  +LP + AF  CT C+P V++ Y+REG   + +V N     LE++TGL  + +ET A E+ W   +DE
Sbjct:  209 SVEVAPL---KDLNSFFPDLKKITVGVYDPCTLPQHPGWPLRNFLVLLAKKWGSQLDVLEVLCFRDRT------------------LQGSRSIRHSIIFRVKLPDLTASAVCP-----KSVGWEKNAKGAMGPRSVNLSECMDPKRLAESSVDLNLKLMRWRLVPSLDLDKVVSTRCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLSGGKAKALAAVDRLKKIFPGVNAEGYNMSIPMPGHPVNFSELTMAQARQDVEQLEKLISEHDVVFLLMDTRESRWLPTVIAASQRKLIVNAALGFDTFVVMRHGLK--KPKESQSEESSPMSASSNSSCSSTPAATITPGASLFSNIPGHRLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAMIAGALAVELMVSVLQHP-------EGGYAVASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPASLAFDKCTACSPIVLDNYEREGFQFLAKVFNSSHSFLEDLTGLTLLHQETQAAEI-WDMSDDE 711          

BLAST of EWM26809.1 vs. NCBI_GenBank
Match: gi|918998964|ref|XP_013397719.1| (PREDICTED: ubiquitin-like modifier-activating enzyme ATG7 [Lingula anatina])

HSP 1 Score: 471.47 bits (1212), Expect = 3.897e-157
Identity = 258/511 (50.49%), Postives = 340/511 (66.54%), Query Frame = 0

Query:  124 VMFGYVDPGTVPDAPGWPLRNFLLLLANRWG--LCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMH-KGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVSSPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQ-PNPPLTSVAPSCSGGEDGGTAGESKARTGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEASGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCNEQGVLEEMTGLAQMRRETDALELSWSEDEDEE 630
            V+F   DP T+ + PGWP+RN L L+A  W   L    +V +R R++                   G++ +R S V +V L  + S+ +  P       GWE + KGK +PR VDLSATMDP++L+E SVDLNLKLMRWRLLP L++  + RT+CLLLGAGTLGC+VAR LLGWGVR IT VDN R++ SNPVRQSLF + DC  GGK KA AAA +LRRIFPGV A+GH +TIPMPGHP+S  + E + R++V +L++LV +HDVIFLL DTRESRWLPT+LGA   KI++NAALGFDTF+V+RHG + P  P+           D G  GE+K  +         G  +GCYFCNDVVAP DST+DRTLDQQCTV+RPG++ + +ALAVEL+V++L HP    APAD    S        D    LG++P QIRG+L+ +  +LP +RAF+ CT C+  VIE Y++EG G ++   N+   LE++TGL Q+ +ET   E+ W+  +D+E
Sbjct:    6 VLFCVCDPSTLAEHPGWPVRNLLTLIAYHWADKLPGVEIVSFRDRISQ------------------GSRDIRHSLVFNVQLQSI-SNLTECP----KCVGWEKNAKGKLVPRMVDLSATMDPVKLSESSVDLNLKLMRWRLLPELNLEKIKRTRCLLLGAGTLGCNVARSLLGWGVRKITFVDNSRISYSNPVRQSLFVFEDCLHGGKAKAEAAAESLRRIFPGVDAQGHSLTIPMPGHPLSESSIEQT-RKDVAVLEELVDQHDVIFLLMDTRESRWLPTLLGAAKGKIVLNAALGFDTFLVVRHGVKTPGEPM-----------DTGPPGEAKLSSN------VHGSQLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGMSMITAALAVELLVSILQHPEEGGAPAD---TSARDEHITKDMMSILGLVPHQIRGFLSRYYNLLPASRAFEKCTACSNIVIEHYRKEGFGFLLSAFNQPSYLEDLTGLTQLHQETLDSEV-WAISDDDE 471          

BLAST of EWM26809.1 vs. NCBI_GenBank
Match: gi|583975915|ref|XP_006783326.1| (PREDICTED: ubiquitin-like modifier-activating enzyme ATG7-like [Neolamprologus brichardi])

HSP 1 Score: 478.404 bits (1230), Expect = 2.326e-156
Identity = 259/526 (49.24%), Postives = 340/526 (64.64%), Query Frame = 0

Query:  118 HGEQPQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWG--LCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMH-KGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVS-SPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAP--------SCSGGEDGGTAGESKARTGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEA-SGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCN-EQGVLEEMTGLAQMRRETDALELSWSEDEDE 629
              +  +V  G  DP T+   PGWPLRN L+L+AN+WG  L    V+C+R R                   L G++ ++ S + HV L  L ++ S+ P     + GWE + KG   PR V+LS  MDP RLAE SVDLNLKLMRWRL+P+LD+  +  TKCLLLGAGTLGC+VAR L+GWGVR IT VDN +++ SNPVRQ L+E+ DC  GGK KA+AA   L +IFPGV AEG+ M+IPMPGHPV+ S A  + A+R+VE L+KL+ EHDV+FLL DTRESRWLPTV+ A   K++VNAALGFDTF+VMRHG +  P + SV+         S +       AG + A  G        G  +GCYFCNDVVAP DST+DRTLDQQCTV+RPGLA +A ALAVE+MV++L H       ++GG A +      +++P   LG++P QIRG+L+ F  +LP + AF  CT C+P V++ Y+REG   + +V N     LE++TGL  + +ET A E+ W   +DE
Sbjct:  224 FSDTKKVTVGIYDPCTLSQHPGWPLRNLLMLVANQWGSKLDTVEVLCFRDRT------------------LQGSRSIQQSVIFHVKLPELPNN-SACP----KSVGWEKNPKGAMGPRMVNLSECMDPKRLAESSVDLNLKLMRWRLVPSLDLDKVINTKCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKSKAMAAVDRLTKIFPGVNAEGYNMSIPMPGHPVNFSQATLSQAQRDVEQLEKLIEEHDVVFLLMDTRESRWLPTVIAASKRKLVVNAALGFDTFVVMRHGLKKPPNIQSVSTEPDSSPSCSSASSSSSTPAGAAPAVPGSSLFSNIPGHKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAMIAGALAVEMMVSILQH-------SEGGYAVASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPASLAFDKCTACSPIVLDHYEREGFSFLSKVFNSSHSFLEDLTGLTLLHQETQAAEI-WDMSDDE 718          

BLAST of EWM26809.1 vs. NCBI_GenBank
Match: gi|548344600|ref|XP_005724719.1| (PREDICTED: ubiquitin-like modifier-activating enzyme ATG7 [Pundamilia nyererei] >gi|554843987|ref|XP_005932358.1| PREDICTED: ubiquitin-like modifier-activating enzyme ATG7 [Haplochromis burtoni])

HSP 1 Score: 478.404 bits (1230), Expect = 2.377e-156
Identity = 261/526 (49.62%), Postives = 339/526 (64.45%), Query Frame = 0

Query:  118 HGEQPQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWG--LCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMH-KGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVS-SPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQ--PNPPLTSVAPSCSGGEDGGT------AGESKARTGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEA-SGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCN-EQGVLEEMTGLAQMRRETDALELSWSEDEDE 629
              +  +V  G  DP T+   PGWPLRN L+L+AN+WG  L    V+C+R R                   L G++ ++ S + HV L  L S+ S+ P     + GWE + KG   PR V+LS  MDP RLAE SVDLNLKLMRWRL+P+LD+  +  TKCLLLGAGTLGC+VAR L+GWGVR IT VDN +++ SNPVRQ L+E+ DC  GGK KA+AA   L +IFPGV AEG+ M+IPMPGHPV+ S A  + A+R+VE L+KL+ EHDV+FLL DTRESRWLPTV+ A   K++VNAALGFDTF+VMRHG +  PN    S  P  S      +      AG + A  G        G  +GCYFCNDVVAP DST+DRTLDQQCTV+RPGLA +A ALAVE+MV++L H       ++GG A +      +++P   LG++P QIRG+L+ F  +LP + AF  CT C+P V++ Y+REG   + +V N     LE++TGL  + +ET A E+ W   +DE
Sbjct:  224 FSDTKKVTVGVYDPCTLSQHPGWPLRNLLMLVANQWGSKLDTVEVLCFRDRT------------------LQGSRSIQQSVIFHVKLPELPSN-SACP----KSVGWEKNPKGAMGPRMVNLSECMDPKRLAESSVDLNLKLMRWRLVPSLDLDKVINTKCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKSKAMAAVDRLTKIFPGVNAEGYNMSIPMPGHPVNFSQATLSQAQRDVEQLEKLIEEHDVVFLLMDTRESRWLPTVIAASKRKLVVNAALGFDTFVVMRHGLKKPPNTQSVSTEPDSSPSCSSASSSSSTPAGAAPAVPGSSLFSNIPGHKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAMIAGALAVEMMVSILQH-------SEGGYAVASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPASLAFDKCTACSPIVLDHYEREGFSFLSKVFNSSHSFLEDLTGLTLLHQETQAAEI-WDMSDDE 718          

BLAST of EWM26809.1 vs. NCBI_GenBank
Match: gi|972973314|ref|XP_006631169.2| (PREDICTED: ubiquitin-like modifier-activating enzyme ATG7 [Lepisosteus oculatus] >gi|972973316|ref|XP_015203665.1| PREDICTED: ubiquitin-like modifier-activating enzyme ATG7 [Lepisosteus oculatus])

HSP 1 Score: 478.019 bits (1229), Expect = 2.433e-156
Identity = 255/523 (48.76%), Postives = 333/523 (63.67%), Query Frame = 0

Query:  113 KMDRSHGEQPQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWG--LCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMH-KGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVS-SPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTAGESKARTGKGSPGPADGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGEA-SGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCN-EQGVLEEMTGLAQMRRETDALELSWSEDEDE 629
            K D    EQ +V  G  DP T+P  PGWPLRN L L+A +WG  L    V+C+R R                   L G + ++ S +  V L  +++S      G   + GWE + KG   PR V+LS  MDP RLAE SVDLNLKLMRWRL+P+LD+  +  TKCLLLGAGTLGC+VAR L+GWGVR IT +DN +++ SNPVRQ L+E+ DC +GGKPKALAA   L +IFPGV A+G+ M+IPMPGHPV+ S A    AR +VE L++L+A HDV+FLL DTRESRWLPTV+ A   K++VNAALGFDTF+V+RHG +      S     +  E   ++    A  G        G  +GCYFCNDVVAP DST+DRTLDQQCTV+RPGLA +A ALAVELM+++L HP       +GG A +      +++P   LG++P QIRG+L+ F  +LP + AF  CT C+P V++ Y+R+G   +  V N     LE++TGL  + +ET A E+ W   +DE
Sbjct:  221 KWDDFFKEQKKVTVGVYDPCTLPQHPGWPLRNLLFLIACKWGGLLDSVEVLCFRDRT------------------LLGTRSIKHSIIFEVELPRVSASA-----GCPKSVGWEKNQKGAMGPRMVNLSECMDPKRLAESSVDLNLKLMRWRLVPSLDLEKIISTKCLLLGAGTLGCNVARTLMGWGVRHITFLDNAKISYSNPVRQPLYEFEDCLNGGKPKALAAVERLEKIFPGVAAKGYNMSIPMPGHPVNFSQATMDQARTDVEQLEQLIASHDVVFLLMDTRESRWLPTVIAASQRKLVVNAALGFDTFVVIRHGLKKPRQAES-----AEAEPMSSSSTYSAVPGTSLFSNIPGHRLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLATIAGALAVELMISILQHP-------EGGYAVASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPASLAFDKCTACSPVVLDHYERDGFQFLSEVFNSSHSFLEDLTGLTLLHKETQAAEI-WDMSDDE 707          

BLAST of EWM26809.1 vs. NCBI_GenBank
Match: gi|1025081222|ref|XP_016340088.1| (PREDICTED: ubiquitin-like modifier-activating enzyme ATG7 isoform X1 [Sinocyclocheilus anshuiensis])

HSP 1 Score: 478.404 bits (1230), Expect = 2.888e-156
Identity = 263/532 (49.44%), Postives = 341/532 (64.10%), Query Frame = 0

Query:  102 SLEVCPLDAYEKMDRSHGEQPQVMFGYVDPGTVPDAPGWPLRNFLLLLANRWG--LCEATVVCYRGRVTGPTHDPEAGGNGSHLNPLGGAQVMRGSFVLHVSLAPLASSPSSSPPGALTTTGWEMH-KGKYMPRRVDLSATMDPIRLAEQSVDLNLKLMRWRLLPALDIAALARTKCLLLGAGTLGCSVARGLLGWGVRTITLVDNGRVALSNPVRQSLFEYTDCADGGKPKALAAAAALRRIFPGVVAEGHVMTIPMPGHPVS-SPAEEASARREVELLDKLVAEHDVIFLLTDTRESRWLPTVLGAVHDKILVNAALGFDTFMVMRHGAQPNPPLTSVAPSCSGGEDGGTAGESKARTGKGSPGPA-----DGDGIGCYFCNDVVAPDDSTKDRTLDQQCTVTRPGLAPVASALAVELMVALLHHPLRHRAPADGGE--ASGLGHRFLDDPDRPLGILPQQIRGYLAHFTTILPVTRAFQHCTGCAPAVIEAYKREGAGLVVRVCN-EQGVLEEMTGLAQMRRETDALEL 621
            S+EV PL   + ++    +  ++  G  DP T+P  PGWPLRNFL+LLA +WG  L    V+C+R R                   L G++ +R S +  V L  L +S          + GWE + KG   PR V+LS  MDP RLAE SVDLNLKLMRWRL+P+LD+  +  T+CLLLGAGTLGC+VAR L+GWGVR IT VDN +++ SNPVRQ L+E+ DC  GGK KALAA   L++IFPGV AEG+ M+IPMPGHPV+ S    A AR++VE L+KL++EHDV+FLL DTRESRWLPTV+ A   K++VNAALGFDTF+VMRHG +   P  S +   S       +  S       +PG +      G  +GCYFCNDVVAP DST+DRTLDQQCTV+RPGLA +A ALAVELMV++L HP       +GG   AS    R +++P   LG++P QIRG+L+ F  +LP + AF  CT C+P V++ Y+REG   + +V N     LE++TGL  + +ET A E 
Sbjct:  209 SVEVAPL---KDLNSFFPDLKKITVGVYDPCTLPQHPGWPLRNFLVLLAKKWGSQLDVLEVLCFRDRT------------------LQGSRSIRHSIIFRVKLPDLTASAVCP-----KSVGWEKNAKGAMGPRSVNLSECMDPKRLAESSVDLNLKLMRWRLVPSLDLDKVVSTRCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLSGGKAKALAAVDRLKKIFPGVNAEGYNMSIPMPGHPVNFSELTMAQARQDVEQLEKLISEHDVVFLLMDTRESRWLPTVIAASQRKLIVNAALGFDTFVVMRHGLK--KPKESQSEESSPMSASSNSSCSSTPAATITPGASLFSNIPGHRLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAMIAGALAVELMVSVLQHP-------EGGYAVASSSDDR-MNEPPTSLGLVPHQIRGFLSRFDNVLPASLAFDKCTACSPIVLDNYEREGFQFLAKVFNSSHSFLEDLTGLTLLHQETQAAEF 704