EWM30221.1, cds382 (CDS) Nannochloropsis gaditana

BLAST of EWM30221.1 vs. NCBI_GenBank
Match: gi|585112880|gb|EWM30221.1| (p21-activated protein kinase [Nannochloropsis gaditana])

HSP 1 Score: 1192.95 bits (3085), Expect = 0.000e+0
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query:    1 MSNTSSDYPVRQQAHAMGALKPRMPEEMMGTLSLEEIPSPPPDNNGGVPHAGSDSLTGEGEPAADTVGTPKTVITAPITSTDSFRLSASESELAGDRSNEPARGTSVSKPMQVKHHIHVSFDPTSGAFKGLPPEWGKHLPVGVSKEEANVTATDRHVAPPKPTAEVKKTLNPLRKFTWWSSGSKGGGDRNPPLAGTVIGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPTHTRGPLPSRAAVEQAMAKALRIVKDDPRQYFSDLKRLGQGASGTVYAATDRRTGERRALKIAPVSELADLTNEIGLQSMSDHPNVVRIYEAFVTTSEVCIVMELMLGGSLTDVLGKHVVDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRAQLEARRRRRQQQAQAQAAHNKGR 579
            MSNTSSDYPVRQQAHAMGALKPRMPEEMMGTLSLEEIPSPPPDNNGGVPHAGSDSLTGEGEPAADTVGTPKTVITAPITSTDSFRLSASESELAGDRSNEPARGTSVSKPMQVKHHIHVSFDPTSGAFKGLPPEWGKHLPVGVSKEEANVTATDRHVAPPKPTAEVKKTLNPLRKFTWWSSGSKGGGDRNPPLAGTVIGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPTHTRGPLPSRAAVEQAMAKALRIVKDDPRQYFSDLKRLGQGASGTVYAATDRRTGERRALKIAPVSELADLTNEIGLQSMSDHPNVVRIYEAFVTTSEVCIVMELMLGGSLTDVLGKHVVDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRAQLEARRRRRQQQAQAQAAHNKGR
Sbjct:    1 MSNTSSDYPVRQQAHAMGALKPRMPEEMMGTLSLEEIPSPPPDNNGGVPHAGSDSLTGEGEPAADTVGTPKTVITAPITSTDSFRLSASESELAGDRSNEPARGTSVSKPMQVKHHIHVSFDPTSGAFKGLPPEWGKHLPVGVSKEEANVTATDRHVAPPKPTAEVKKTLNPLRKFTWWSSGSKGGGDRNPPLAGTVIGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPTHTRGPLPSRAAVEQAMAKALRIVKDDPRQYFSDLKRLGQGASGTVYAATDRRTGERRALKIAPVSELADLTNEIGLQSMSDHPNVVRIYEAFVTTSEVCIVMELMLGGSLTDVLGKHVVDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRAQLEARRRRRQQQAQAQAAHNKGR 579          

BLAST of EWM30221.1 vs. NCBI_GenBank
Match: gi|299469753|emb|CBN76607.1| (Possible protein kinase [Ectocarpus siliculosus])

HSP 1 Score: 535.413 bits (1378), Expect = 0.000e+0
Identity = 262/460 (56.96%), Postives = 328/460 (71.30%), Query Frame = 0

Query:  113 VKHHIHVSFDPTSGAFKGLPPEWGKHLPVGVSKEEANVTAT-------DRHVAPPKPTAEVKKTLNPLRKFTWWSSGSKGGGDRNPPLAGTVIGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPTHT--RGPLPSRAAVEQAMAKALRIVKDDPRQYFSDLKRLGQGASGTVYAATDRRTGERRALKIAPVSELADLTNEIGLQSMSDHPNVVRIYEAFVTTSEVCIVMELMLGGSLTDVLGKHVVDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRAQLEARRR 563
            V+ H+HV +DP SG FKG+P EW   LP G + +     A        + H+A P PT E K      R+F  W  G   G +   P  G+VIGAPFNVQH THV+PD  SSTGF+GLP  W  VLK SGITK+E V+HPQAVL AL FHM GP     R  +PS+ A+ + + +A+ + KDDP Q+++D+++LGQGASGTV+  TD RTGER ALK  PV+E+ DL NEIG+Q +S HPN+V + EAFVT +EV I M+ M GGSLTD LG   VDFKE +IAYV + +L ALA+MH  +R+HRDIKSDNVLV+ EG+VK+ADFGFA ALT E  KR SVVGTPYWMAPE+I+G +YD KVDVWS+GIT +EMAEGEPP LHEPPLRALL+ISI+ SP LKDP++WS  F+HFLA S  V+ E R TA+QLL HPF+  A ++ E+ A VR  L  +++
Sbjct:   27 VQKHLHVEWDPASGTFKGMPEEWANLLPEGTAVQTKAAPAVSPKHAKRNSHIAAPVPTVEAK------RRFRLW--GRNDGKEPEEPSVGSVIGAPFNVQHVTHVKPDSRSSTGFTGLPADWHVVLKASGITKEEVVEHPQAVLDALAFHMDGPQAKAQRPKMPSKEAIAKTVTEAMPLKKDDPMQFYTDMRKLGQGASGTVFVGTDVRTGERCALKFCPVAEMDDLKNEIGMQCLSKHPNIVTLREAFVTKTEVVIAMDFMEGGSLTDTLGT-TVDFKEMYIAYVCREILLALAFMHHQFRLHRDIKSDNVLVNLEGKVKLADFGFAAALTAEQDKRASVVGTPYWMAPEIIKGLEYDGKVDVWSMGITALEMAEGEPPLLHEPPLRALLLISINPSPRLKDPNKWSRGFIHFLASSLDVEVEKRYTADQLLQHPFIQTACSEAEFSAHVRKMLAKKKK 477          

BLAST of EWM30221.1 vs. NCBI_GenBank
Match: gi|855314366|ref|XP_012898492.1| (uncharacterized protein [Blastocystis hominis] >gi|300123171|emb|CBK24444.2| unnamed protein product [Blastocystis hominis])

HSP 1 Score: 367.851 bits (943), Expect = 2.423e-119
Identity = 176/371 (47.44%), Postives = 250/371 (67.39%), Query Frame = 0

Query:  198 IGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPTHTRGPLPSRAAVEQAMAKALRIVKDDPRQYFSDL-KRLGQGASGTVYAATDRRTGERRALKIAPVSELADLTNEIGLQSMSDHPNVVRIYEAFVTTSEVCIVMELMLGGSLTDVLGKHVVDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRAQLEARRRRRQQ 567
            +  P N Q   HV  DP S  G++GLP  W   LK + IT+  A+ +  A+   L   +  P +   PLP+    ++ + KA  I ++DP +++  + +R+G+GASG VY  T   TGER ALK++P S+L +L NE+ +Q + +HPN+V + + F+  +++ I MELM  G LT++LG+ + DFKE +IAYV   +L AL+Y+H + ++HRDIKSDNVL++ +G++K+ DFGFAI LTKE  +R SVVGTP+WMAPELIRG +Y+  VD+WSLGITC+EMA+GEPP+  EPPLRALL+I  S  P +KDP RWS QFL FL +   ++   R TA Q+LNHPFL MA + +E+  F    L+   RRR+Q
Sbjct:   36 VSNPRNFQAMHHVALDPDSELGWTGLPEEWIPYLKANNITQDMAIKNKDAIADCLTL-LNDP-YRPMPLPNAEEWDRELDKASFIRREDPFEFYHGINQRIGEGASGVVYKGTSTETGERVALKVSPASDLQNLKNELAMQRLCNHPNLVGLRDCFLWNNQLWIAMELMDAGCLTEILGQDI-DFKEKYIAYVCLNILQALSYLHRNNKLHRDIKSDNVLINSKGEIKLGDFGFAIGLTKEQDRRKSVVGTPFWMAPELIRGAEYNGSVDIWSLGITCLEMADGEPPYYREPPLRALLLIHTSPPPTVKDPLRWSPQFLDFLKRCLELNPVERGTANQMLNHPFLKMACSPKEFANFA---LDILNRRREQ 400          

BLAST of EWM30221.1 vs. NCBI_GenBank
Match: gi|1032150416|gb|OAO14198.1| (p21-activated protein kinase [Blastocystis sp. ATCC 50177/Nand II])

HSP 1 Score: 362.844 bits (930), Expect = 2.651e-117
Identity = 180/394 (45.69%), Postives = 252/394 (63.96%), Query Frame = 0

Query:  172 PLRKFTWWSSGSKGGGDRNPPLAGTVIGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPTHTRGPLPSRAAVEQAMAKALRIVKDDPRQYFSDL-KRLGQGASGTVYAATDRRTGERRALKIAPVSELADLTNEIGLQSMSDHPNVVRIYEAFVTTSEVCIVMELMLGGSLTDVLGKHVVDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRAQLEARRRR 564
            P +K T  +SG  G G R        +  P N +   HV  DP S  G  GLP  W+  L+ S I+K  A+ + +A++    F      +   PLP  +   + + +A  I ++DP + + ++ +RLG+GASGTVY  TD  TGER ALK+AP S+L +L NE+ LQ M +  N+V I + ++   ++ I MELM  G LT++LG  + DF E HIAYV K VL+AL+Y+H + ++HRDIKSDNVL++ +G++K+ADFGFA+ LT+E ++R SVVGTP+WMAPELIRGT YD  VD+WSLGIT +EMA+GEPP+  EPPLRALLMI  S SP + +P +WS +FL FL +   +D   R TA+++L HPF+  A T   +  F    L  RR +
Sbjct:   18 PGKKTTHRASGWFGFGRRQKKWVPVEVSKPSNFKSMNHVEIDPNSELGLKGLPDNWKVWLQTSNISKDMAMKNKEALVDC--FTYMDSQNKPAPLPKVSDFAEELRQAAFIREEDPLKVYVNINQRLGEGASGTVYKGTDSITGERIALKVAPASDLKNLKNELALQRMCNQQNIVSIRDCYLWNDKLWIAMELMDAGCLTEILGPDI-DFPETHIAYVCKNVLTALSYLHHNNKLHRDIKSDNVLMNTKGEIKLADFGFAVGLTQEENRRKSVVGTPFWMAPELIRGTAYDGSVDIWSLGITALEMADGEPPYYREPPLRALLMIHTSPSPTVHNPDKWSPEFLDFLKRCLELDPSKRGTADEMLKHPFVEKACTPAAFAQFALQILNERRNQ 408          

BLAST of EWM30221.1 vs. NCBI_GenBank
Match: gi|551636025|ref|XP_005820841.1| (hypothetical protein GUITHDRAFT_81068 [Guillardia theta CCMP2712] >gi|428164849|gb|EKX33861.1| hypothetical protein GUITHDRAFT_81068 [Guillardia theta CCMP2712])

HSP 1 Score: 324.324 bits (830), Expect = 2.181e-102
Identity = 163/361 (45.15%), Postives = 229/361 (63.43%), Query Frame = 0

Query:  198 IGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPTHTRGPLPSRAAVEQAMAKALRIVKDDPRQYFS-DLKRLGQGASGTVYAAT-DRRTGERRALKIAPVSELADLTNEIGLQSMSDHPNVVRIYEAFVTTSEVCIVMELMLGGSLTDVLGKHVVDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRA 556
            I APFN QH  HV+ DP+SSTGF+GLPP W  +L+ + ITK+EA++H  A+L  L F  +     + P   +A++E  MA  ++ + ++P  ++    K +GQG  GT+Y  T D +    + L ++  ++L  L NEI +   S HPNVV  +E+++    + +VMELM GGSLT+++ K   +F E  +A   KA + A+A++H   R+HRDIKSDNVL++  G+VKIADFGF + LT+E   R S+VGTPYWMAPEL+RG  YD KVDVWSLGI  IEMAE EPP+L E PLRAL +I+  G P LK  S++   F+ F  +  +     R TA +L NHPFL  AS+ E     + +
Sbjct:   36 ISAPFNFQHKYHVQVDPSSSTGFTGLPPGWDGLLQQANITKKEALEHGDAILDILRFQQEQGCEPQLPTAQQASMEAKMA--VQFLNENPADHYKMPPKSIGQGGMGTIYRGTKDGKDYAIKKLALSKQTDLPALQNEIAMMKTSAHPNVVEYFESYMFDRCLWVVMELMDGGSLTNLIQKRK-EFTEEEMAGFMKASIDAIAFLHGQGRIHRDIKSDNVLINSRGEVKIADFGFCVQLTQEKDMRQSMVGTPYWMAPELVRGQTYDQKVDVWSLGIMLIEMAESEPPYLREQPLRALYLIATKGEPKLKQESKYRGTFMDFYHKCLNPLPAKRATAAELQNHPFLACASSPEHLAEIINS 393          

BLAST of EWM30221.1 vs. NCBI_GenBank
Match: gi|950897194|ref|XP_014529233.1| (P21-activated protein kinase [Blastocystis sp. subtype 4] >gi|902863834|gb|KNB45780.1| P21-activated protein kinase [Blastocystis sp. subtype 4])

HSP 1 Score: 325.094 bits (832), Expect = 2.671e-102
Identity = 161/369 (43.63%), Postives = 234/369 (63.41%), Query Frame = 0

Query:  198 IGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPTHTRGPLPSRAAVEQAMAKALRIVKD-DPRQYFSDLKR-LGQGASGTVYAATDRRTGERRALKIAPVSELADLTNEIGLQSMSDHPNVVRIYEAFVTTSEVCIVMELMLGGSLTDVLGKHVVDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRAQLEARRRR 564
            I +P++ +H  H+  D  S  G SGLP  WR +L+ S I+K + +++ +AV+ AL +  +       P         AM +    +++ DP++Y+  LK  LG+G   TVY AT+ +T E  A+K+  +SEL ++  E+ LQ    H N++ + + F    ++ IVME M  GSL  ++GK V  F+E +IAYV K +LSAL+ MH   R+HRDIKSDN+L++ +G+VK+ADFGFA+ L  +  +R SVVGTP+WMAPELI+G  Y+S VD+WSLGIT +EMA+GEPPH H+ PL+ALL+I  S SP + +P +WS  F  +LA+S   + E R TA +LL HPFL  A   EE+  FV   L  R+ +
Sbjct:   62 IESPYDFRHNMHIYVDVNSPCGLSGLPDEWRLLLESSNISKDDVINNGEAVINALAYQFKPEV---IPTAQDMLDMDAMLRDKSFIREEDPKEYYRGLKEVLGEGGYSTVYKATNIKTEEVVAVKVTSLSELLNIRQELALQKTCHHSNIMTVGDCFNWKDKLWIVMEYMEKGSLFGIVGKDV-HFEEKYIAYVCKGILSALSSMHHDNRIHRDIKSDNILINGKGEVKLADFGFAVRLASDKERRKSVVGTPFWMAPELIQGGGYNSSVDIWSLGITALEMADGEPPHYHDAPLKALLLIHTSPSPTVLEPEKWSDDFKDYLARSLDTNPEKRATANELLQHPFLKQACNAEEFLLFVNDILAKRKNQ 426          

BLAST of EWM30221.1 vs. NCBI_GenBank
Match: gi|330797360|ref|XP_003286729.1| (hypothetical protein DICPUDRAFT_31335 [Dictyostelium purpureum] >gi|325083327|gb|EGC36783.1| hypothetical protein DICPUDRAFT_31335, partial [Dictyostelium purpureum])

HSP 1 Score: 319.316 bits (817), Expect = 1.296e-100
Identity = 160/384 (41.67%), Postives = 233/384 (60.68%), Query Frame = 0

Query:  198 IGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPTHTRGPLPSRAAVEQAMAKALRIVKDDPRQYFSD-LKRLGQGASGTVYAATDRRTGERRALKIAPVSE--LADLTNEIGLQSMSDHPNVVRIYEAFVTTSEVCIVMELMLGGSLTDVLGK-HVVDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRAQLEARRRRRQQQAQAQAAHNK 577
            +G+PFNV+H  HV  D  + TGF GLP  W  +L+ SGITK+E   + +AVL  L+FHM            +   E  ++    + ++DP + F +   ++G+GA+G V+  T  RT  + A+K  P+++  +  +  EIG+     H N++   ++++    + + ME M GG LT++L + + V   E  IA+V    L  LAY+HS +R+HRDIKSDN+L+  +G VK+ADFG+A  LTK   KR ++VGTPYWMAPELIRG +YD KVD+WSLGI  +EMAE EPP++  PPLRAL +I+  G P LKD ++WS +F  F+A+    + E RP A+ LLNHPFL  A         V A +EA++        A+ AH+K
Sbjct:    8 VGSPFNVKHNIHV--DYNTVTGFEGLPREWEIILQSSGITKEEVCANSEAVLDVLDFHMNQQQREAQIAQMQNTDETNVSLYDLVSQEDPTKLFGEGSTKIGEGAAGEVFVVTQLRTNNKVAIKKMPLNQQNMKLIVTEIGIMKSCKHQNIIDYIDSYLVGDSLWVAMEFMGGGCLTEILEQFNTVKLNESQIAFVCAETLKGLAYVHSQHRIHRDIKSDNILLGSDGSVKLADFGYAAQLTKSKQKRVTIVGTPYWMAPELIRGQNYDRKVDIWSLGIMAMEMAESEPPYMSFPPLRALFLITTKGIPELKDQNKWSPEFKDFVAKCLDKNVETRPDAKTLLNHPFLKTACNS---SGLVPAIIEAKK--------AKEAHSK 378          

BLAST of EWM30221.1 vs. NCBI_GenBank
Match: gi|1160574971|ref|XP_020427714.1| (p21-activated protein kinase [Polysphondylium pallidum PN500] >gi|281201368|gb|EFA75580.1| p21-activated protein kinase [Polysphondylium pallidum PN500])

HSP 1 Score: 321.242 bits (822), Expect = 5.257e-100
Identity = 162/374 (43.32%), Postives = 230/374 (61.50%), Query Frame = 0

Query:  196 TVIGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPTH-----TRGPLPSRAAVEQAMAKALRIVKDDPRQYFSDLKRLGQGASGTVYAATDRRTGERRALK-IAPVSELADL-TNEIGLQSMSDHPNVVRIYEAFVTTS-EVCIVMELMLGGSLTDVLGKHV-VDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRAQLEA 560
            + +G PFN++H  HV  D  S+TGFSGLP  W  +LK + ++K E +D P   L+ L F     T      T  PLP  +   Q ++    + K+DP + + ++ ++G+GA+G V+ AT  +  +R A+K I   +E A L   EI +   S H N+V   ++++    E+ + ME M GG LTD+L     V   EP IAYV +  L +L Y+HS +R+HRDIKSDN+L+  EG VKIADFG+A  LT++  KR++VVGTPYWMAPELIRG DY  KVD+WSLGI  +EMAEGEPP++  PPLRAL +I+  G P LK+ S+WS  FL F A+   ++ + RP A  LL HPF+  A    ++   ++   E+
Sbjct:  122 STVGTPFNLKHIVHV--DFNSATGFSGLPKEWEVILKSNNVSKDEVLDKPNEWLSVLEFQAGRTTEKTASGTVSPLPDES--NQTLSDL--VSKEDPTKIYKNMTKIGEGAAGEVFVATSSKNNKRVAIKKIEINNENAKLLVTEIAIMKTSQHDNIVNYMDSYILNDRELWVAMEFMGGGCLTDILEAFDHVKMTEPQIAYVVRETLKSLQYIHSLHRIHRDIKSDNILLGSEGSVKIADFGYAAQLTQKQQKRNTVVGTPYWMAPELIRGHDYGVKVDIWSLGIMMMEMAEGEPPYMDFPPLRALFLITTKGIPPLKEQSKWSKDFLDFFAKCLDINVQTRPDASALLRHPFMDKACDGSQFKPLIQLSRES 489          

BLAST of EWM30221.1 vs. NCBI_GenBank
Match: gi|330790787|ref|XP_003283477.1| (hypothetical protein DICPUDRAFT_91113 [Dictyostelium purpureum] >gi|325086587|gb|EGC39974.1| hypothetical protein DICPUDRAFT_91113 [Dictyostelium purpureum])

HSP 1 Score: 318.546 bits (815), Expect = 4.612e-99
Identity = 159/366 (43.44%), Postives = 225/366 (61.48%), Query Frame = 0

Query:  196 TVIGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQGPT--HTRGPLPSRAAVEQAMAKALRIVKDDPRQYFSDLKRLGQGASGTVYAATDRRTGERRALKIAPVSE--LADLTNEIGLQSMSDHPNVVRIYEAFVT-TSEVCIVMELMLGGSLTDVL-GKHVVDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVR 555
            + +  PFN++H  HV  D  S+TGFSGLP  W  +LK S ++KQE +D P   L+ L F   G T   T+    S+   E     +  + K+DP + + ++ ++G+GA+G V+ AT  +  +R A+K   ++      L  EI +   S H N+V   ++++    E+ + ME M GG LTD+L     V   EP IAYV +  L AL Y+HS +R+HRDIKSDN+L+  EG VKIADFG+A  LT++  KR++VVGTPYWMAPELIRG DY  KVD+WSLGI  +EMAEGEPP++  PPLRAL +I+  G P LK+ S+WS  F  F ++   ++ + RP A  LL HPF+ MA    E+   ++
Sbjct:  111 STVSQPFNLKHEVHV--DFNSATGFSGLPKEWEVILKSSNVSKQEVLDKPSEWLSVLEFQ-AGRTMDKTKSQQMSQLPDESNYTLSELVSKEDPTKIYKNMTKIGEGAAGEVFVATSSKNNKRVAIKKIEINNDNAKLLVTEIAIMKTSHHENIVNYIDSYIVGDKELWVAMEFMGGGCLTDILEAFDTVKMNEPQIAYVVRETLKALQYIHSLHRIHRDIKSDNILLGSEGSVKIADFGYAAQLTQKQQKRNTVVGTPYWMAPELIRGHDYGVKVDIWSLGIMMMEMAEGEPPYMDFPPLRALFLITTKGIPPLKEGSKWSKTFQDFFSKCLDINVQNRPDATDLLKHPFIEMACDPLEFKPLIQ 473          

BLAST of EWM30221.1 vs. NCBI_GenBank
Match: gi|831758985|ref|XP_012749509.1| (hypothetical protein SAMD00019534_106200 [Acytostelium subglobosum LB1] >gi|735994275|dbj|GAM27444.1| hypothetical protein SAMD00019534_106200 [Acytostelium subglobosum LB1])

HSP 1 Score: 330.102 bits (845), Expect = 5.631e-99
Identity = 165/388 (42.53%), Postives = 237/388 (61.08%), Query Frame = 0

Query:  198 IGAPFNVQHTTHVRPDPTSSTGFSGLPPAWRAVLKVSGITKQEAVDHPQAVLAALNFHMQ----------GPTHTRGPLPSRAAVEQAMAKALRIVKDDPRQYFSDLKRLGQGASGTVYAATDRRTGERRALKIAPVSE--LADLTNEIGLQSMSDHPNVVRIYEAFVTTSEVCIVMELMLGGSLTDVLGKHV-VDFKEPHIAYVAKAVLSALAYMHSSYRMHRDIKSDNVLVDKEGQVKIADFGFAIALTKETSKRHSVVGTPYWMAPELIRGTDYDSKVDVWSLGITCIEMAEGEPPHLHEPPLRALLMISISGSPALKDPSRWSSQFLHFLAQSTSVDAEARPTAEQLLNHPFLTMASTQEEYGAFVRAQLEARRRRRQQQAQAQ 572
            +G PFNV+H  HV  +  + TGF GLP  W  ++K S   + E +  P+ VL  + FH Q           P+  + PL   A V      +L    DDPR+ + ++ ++G+G +G V+ AT+ RT    A+K   +    L  + NEIG+   S+H N+V+  ++++   E+ + ME M GG LT+VL +H  V   EP IA+V   VL  LAY+H   R+HRDIKSDN+L+  +GQ+K+ADFG+A  LT+   +R+SVVGTPYWMAPELI+G +YD KVDVWSLGI   EMAEGEPP+L  PPLRAL +++  G P +KD   +S +F  FL++    D E RP+A +LLNHPF++ A + +E+   V A   A+ ++ +Q  Q Q
Sbjct:  526 VGIPFNVKHDVHV--NFNADTGFEGLPKEWEVLIK-SNFQENEVMAAPEEVLNVVKFHAQYNNLESAPAPNPSQMQAPLTDEAPVTLNELISL----DDPRKIYININKIGEGGAGEVFEATNTRTNTTIAIKKMKLKAQVLKTVINEIGMMKNSNHANIVQYVDSYIVADELWVAMEFMGGGCLTEVLDQHRDVQLTEPQIAFVCHEVLKGLAYIHKFNRIHRDIKSDNILIGVDGQIKLADFGYAAQLTQNRQQRNSVVGTPYWMAPELIKGNNYDFKVDVWSLGILTREMAEGEPPYLEYPPLRALFLLTTQGLPPIKDAENYSKEFNDFLSRCLEKDTENRPSAAELLNHPFVSKACSGQEFYRAVEASKIAKDKQIEQLNQFQ 906