EWM27003.1, cds3660 (CDS) Nannochloropsis gaditana

BLAST of EWM27003.1 vs. NCBI_GenBank
Match: gi|585109173|gb|EWM27003.1| (dna topoisomerase iii [Nannochloropsis gaditana])

HSP 1 Score: 963.755 bits (2490), Expect = 0.000e+0
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query:    1 MDLRRDKSVLFLACETCAASHLLPHSRKGTSRPSTLTPPVLCPLCGFEALLVTPEGGGREGGGGDERVPPPYHMCPWCYHHPPASGTAMDNFRCANCTGVLDRGLEGREKDGGGGVRGPCPLARAVLDADKAVLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACILCDPLLREHCRYRFSPETPASSACKGRGIEGRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWADDVGGGGSEVPPPGRGAPDMGWNGTRTGNRDEGNGKNITGGGWKRSSTSSLAEGGWQSASRGGGRGAGRGRRGRGGASGGKKAKVSHEMDW 483
            MDLRRDKSVLFLACETCAASHLLPHSRKGTSRPSTLTPPVLCPLCGFEALLVTPEGGGREGGGGDERVPPPYHMCPWCYHHPPASGTAMDNFRCANCTGVLDRGLEGREKDGGGGVRGPCPLARAVLDADKAVLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACILCDPLLREHCRYRFSPETPASSACKGRGIEGRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWADDVGGGGSEVPPPGRGAPDMGWNGTRTGNRDEGNGKNITGGGWKRSSTSSLAEGGWQSASRGGGRGAGRGRRGRGGASGGKKAKVSHEMDW
Sbjct:    1 MDLRRDKSVLFLACETCAASHLLPHSRKGTSRPSTLTPPVLCPLCGFEALLVTPEGGGREGGGGDERVPPPYHMCPWCYHHPPASGTAMDNFRCANCTGVLDRGLEGREKDGGGGVRGPCPLARAVLDADKAVLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACILCDPLLREHCRYRFSPETPASSACKGRGIEGRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWADDVGGGGSEVPPPGRGAPDMGWNGTRTGNRDEGNGKNITGGGWKRSSTSSLAEGGWQSASRGGGRGAGRGRRGRGGASGGKKAKVSHEMDW 483          

BLAST of EWM27003.1 vs. NCBI_GenBank
Match: gi|1188120400|ref|XP_020782808.1| (DNA topoisomerase 3-alpha [Boleophthalmus pectinirostris])

HSP 1 Score: 96.6709 bits (239), Expect = 1.574e-17
Identity = 84/301 (27.91%), Postives = 121/301 (40.20%), Query Frame = 0

Query:  133 VLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACIL-CDPLLREHCRYRFSPETPASSACKGRGIEGRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWADDVGGGGSEVPPPGRGA---PDMGWNGTRTGNRDEGNGKN 429
            V  C  C     +  ++  R   L C G+PACK  ++F   V EV   D+  C  CR             V +L+F+F +  +PP  P  +  CI  CD  LRE    ++            + + G  G G   +           P  P ++A    AP G    R+ N  P+ P++ SS         G     R   +D             A  C+CGQ A +L VRK+GPN G++FY C       G C FF WA++    GS   PP R +   P +G+       R  G+G +
Sbjct:  631 VRKCPNCGRDMVLKKKREGRNKYLSCTGYPACKTAVWFPDMVLEVN-RDDSICPTCRPHP----------VHMLKFKFRRGSLPPMMPLEFVGCIGGCDETLREVLDLKYL-----------KSVGGGGGNGGADSH----------PAAPTKTAPTRAAPSGPASTRNSNSRPLPPVAVSSWTPPPQPPPGGIVNGRINSND-------------AIVCNCGQNAVLLTVRKDGPNQGRQFYKCN-----AGSCNFFLWANECAQQGSSALPPPRNSQPRPSLGFKNASGDGRSRGSGDH 881          

BLAST of EWM27003.1 vs. NCBI_GenBank
Match: gi|930560789|gb|KPI84912.1| (putative DNA topoisomerase III [Leptomonas seymouri])

HSP 1 Score: 93.2041 bits (230), Expect = 2.291e-16
Identity = 113/409 (27.63%), Postives = 150/409 (36.67%), Query Frame = 0

Query:    1 MDL-----RRDKSVLFLACETCAASHLLPHSRKGTSRPSTLTPPVLCPLCGFEALLVTPEGGGREGGGGDERVPPPYHMCPWCYHHPPASGTA------MDN---FRCANCTGVLDRGLEGREKDGGGGVRGPCPLARAVLDADKAVLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACILCDPLLREHCRYRFSPETPASSACKGRGIEGRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQ-GKGTRGMGGG-GHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWAD 393
            MDL      RD+ V  + C  C+ S+ +P+ R     P  LTPP  CPLCGFE L VT     RE           + +CP+C+ HPPAS +       M+    FRC  CT                     CPLA+ +      +  C  C+ Q+ + LR +  G  L C+G+P C   +    A   V+    + C  C                LL F F   +  PG   V T C+ CD  ++++   +  P    SSA    G +  R   S                           P  A+       P +   + S    GG        R      D  G G    G G G    C CG  AK L+ RKE  + GK F  C     A   CGFFQW D
Sbjct:  644 MDLVEHQGERDREVWAVRCGRCSKSYRVPNCRLNKLEP--LTPPQRCPLCGFEVLRVT----NREKQTS-------HTICPYCFSHPPASASVNGMLPDMETAGEFRCFQCT-------------------ADCPLAKGL--ESIGITPCIACR-QNELRLRASSCGYFLSCKGYPTCNLIVSLPTAT-SVKPCPTQRCPSC-------------NAVLLIFDFRGVQGVPGLSPVDTICVSCDARIKDYICVKGFPAAGVSSA-DTDGNDNNRKNAS---------------------------PSAAY-----TLPSVAAAAASGRRGGGSGGGRGRGRSGGGYHDHAGTGASTFGSGDGANVMCGCGMPAKQLVSRKEA-SKGKRFLTC-----ANRQCGFFQWLD 964          

BLAST of EWM27003.1 vs. NCBI_GenBank
Match: gi|290980003|ref|XP_002672722.1| (predicted protein [Naegleria gruberi] >gi|284086301|gb|EFC39978.1| predicted protein [Naegleria gruberi])

HSP 1 Score: 91.2781 bits (225), Expect = 8.534e-16
Identity = 98/420 (23.33%), Postives = 151/420 (35.95%), Query Frame = 0

Query:    1 MDLRRDKSVLFLACETCAASHLLPHSRKGTSRPSTLTPPVLCPLCGFEALLVTPEGGGREGGGGDERVPPPYHMCPWCYHHPPA---SGTAMDN-FRCANCTGVLDRGLEGREKDGGGGVRGPCPLARAVLDADKAVLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQ-RKVPPGTPEVYTACILCDPLLREHCRYRFSPETPAS------------SACKGRGIEGRR---------GRGS-VGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWAD 393
            MDL+      FL C+ C+ S+ LP      S+    +   +CP+C F+ L +  +                Y +CP C+++PP    S  +  N F+C NCT                     C  +R     D  V  C+ C     +  RKN+  L +GC+GFP CK  ++   ++ +  +      E+C      R   +   V  L+F F Q   +PPG    +T C+ CD    E      +     S            S    R +  R           R S VGA     A  Q   PPP +  +      G   R D         + +   N     +  + R      +Q     G  G G     +C  A ++    K+G N G++FY C    + +    FF+W D
Sbjct:  656 MDLKEQDGNNFLHCKNCSDSYPLP------SKSEYESIEDVCPICNFQILNIITQKNT------------SYKLCPHCFNNPPEIENSNESFANGFKCFNCTFT------------------GCKYSRK---KDAVVRKCQKCSNNLVVRQRKNDTSLYIGCKGFPNCKSAVWLPKSIEKADLT----TEKC-----NRCSTRSDTVYRLKFTFAQGTLLPPGVNREHTTCVFCDQDFDEAFNINLNSLNGDSGGASSHGVLVHNSTSNNRNMPNRSPTIAGGGYGNRTSPVGATGRNIAARQNNTPPPNQMTLDRML--GKRTRVD---------NNNGNNNSNPPPQNSYQRNNTSPRNQFGNNHGGSGSGDVVCTNCSSAPEIFTC-KQGENQGRQFYKCPNKDKDEACKKFFKWVD 1015          

BLAST of EWM27003.1 vs. NCBI_GenBank
Match: gi|597768109|ref|XP_007247892.1| (PREDICTED: DNA topoisomerase 3-alpha [Astyanax mexicanus])

HSP 1 Score: 90.1225 bits (222), Expect = 2.039e-15
Identity = 77/266 (28.95%), Postives = 107/266 (40.23%), Query Frame = 0

Query:  133 VLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACIL-CDPLLREHCRYRFSPETPASSACKGRGIE---GRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWADD 394
            V  C  C     +  RK+  G  L C G+PACK  ++F   V EV   D+  C  CR             V +L+F+F +  +PPG    +  CI  CD  LRE    ++     +      + I+    R+            +  +  PPPP  SAV              +RPP+   + S PM             +   SDQ           +A  C+CG  A +L VRKEGPN G++FY C       G C FF WAD+
Sbjct:  654 VRKCPQCNRDMVLRKRKDSTGKFLSCLGYPACKAAVWFPDTVLEVS-RDDSVCPTCRPHP----------VHMLKFKFKRGSLPPGMDLEFVGCIGGCDETLREVLNLKYLRGGGSGGDSGIQVIQRTASRQPNAPRNNNTALQSRSENRPPPPRLSAVS-------------SRPPLTNPTQSRPM-------------QLPSSDQS----------NAVVCNCGVDALLLTVRKEGPNQGRQFYKCNT-----GNCKFFLWADE 867          

BLAST of EWM27003.1 vs. NCBI_GenBank
Match: gi|1049239595|ref|XP_017558650.1| (PREDICTED: DNA topoisomerase 3-alpha [Pygocentrus nattereri])

HSP 1 Score: 88.1965 bits (217), Expect = 8.586e-15
Identity = 77/262 (29.39%), Postives = 100/262 (38.17%), Query Frame = 0

Query:  133 VLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACIL-CDPLLREHCRYRFSPETPASSACKGRGIEGRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWAD 393
            V  C  C     +  RK   G  L C G+PACK  ++F   V EV   D+  C  CR             V +L+F+F +  +PPG    +  CI  CD  LRE    ++                 R G  S        A  Q  PP              A + R ENRPP   L+  S           W  + +               G+   C+CGQ A +L VRKEGPN G++FY C     + G C FF WAD
Sbjct:  654 VRKCLQCNLDMVLKKRKEGTGKFLSCMGYPACKAAVWFPDTVLEVS-RDDSICPTCRPHP----------VHMLKFKFKRGSLPPGMDLEFVGCIGGCDETLREVLNLKYL----------------RGGGDSATQVNHSLASHQSNPP----------RANAALQSRSENRPPPPRLNTDSSRPPLPSSTQAWPMQPSSSVQ-----------GNTIVCNCGQDALLLTVRKEGPNQGRQFYKC-----STGNCKFFLWAD 862          

BLAST of EWM27003.1 vs. NCBI_GenBank
Match: gi|345305221|ref|XP_001510142.2| (PREDICTED: DNA topoisomerase 3-alpha [Ornithorhynchus anatinus])

HSP 1 Score: 86.2705 bits (212), Expect = 3.657e-14
Identity = 79/269 (29.37%), Postives = 110/269 (40.89%), Query Frame = 0

Query:  133 VLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACIL-CDPLLREHCRYRFSPETPASSACKGRGIEGRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWADDVGGGGS 400
            +  C  C     +  +KN  G  LGC G+PACK  ++F  +V EV   D+  C +C              V  L+F+F +  +PP  P  +  CI  CD  LRE    R+  + P  +      ++  +    +G+        QP PPP  R                 NRPP    +PSS    G     P A                  G ++  C+CGQ A +L VRK+GPN G++FY C       G C FF WAD    GG+
Sbjct:  650 IRKCPQCNKDMVLKTKKN-GGFYLGCLGYPACKAAVWFPDSVLEVSK-DDSVCPDCVPHP----------VYKLKFKFKRGSIPPTMPLEFVGCIGGCDETLREILDLRYF-QGPPRALHPASHVQLNQSLNRMGSRYNH----QPRPPPEGR-----------------NRPP----NPSS----GTHLSFPAA----------------AEGSNSVMCNCGQEALLLTVRKDGPNQGRQFYKC-----GAGACNFFLWADQQAEGGA 855          

BLAST of EWM27003.1 vs. NCBI_GenBank
Match: gi|944341522|ref|XP_014375638.1| (PREDICTED: DNA topoisomerase 3-alpha, partial [Alligator sinensis])

HSP 1 Score: 86.2705 bits (212), Expect = 4.248e-14
Identity = 79/280 (28.21%), Postives = 109/280 (38.93%), Query Frame = 0

Query:  132 AVLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACIL-CDPLLREHCRYRFSPETPASSACKGRGIEGRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWADDVGGGGSEVPPPGRGAP 410
            A+  C  C     +  RKN  G  LGC G+PACK  ++F   V EV   D+  C  C      R          L+F+  Q   PP  P  +  CI  CD LLR+    ++    P  ++  G+     +   S+                  R A  E        RR    PPI  L P       G +    A + A  +D+           +A  C+CGQAA +L VRK+GPN G++FY C     +   C FF WAD      +   P    +P
Sbjct:  607 AIRKCPRCNNDMVLKTRKNG-GFYLGCVGYPACKTAVWFPDFVLEVSK-DDSVCAVCEPHPVHR----------LKFKLRQGSAPPLLPLEFVGCIGGCDKLLRDTLDLQYLQRLPQPASEAGQADNHLQASHSLN-----------------RMASNENG-----HRRPATNPPIGFLGPR------GTRPPSTAARLAVAADEN----------NAVMCNCGQAAVLLTVRKDGPNQGRQFYKC-----SASSCNFFLWADQPAEDRNSAAPRSFASP 831          

BLAST of EWM27003.1 vs. NCBI_GenBank
Match: gi|1042324601|ref|XP_017309955.1| (PREDICTED: DNA topoisomerase 3-alpha isoform X1 [Ictalurus punctatus])

HSP 1 Score: 86.2705 bits (212), Expect = 4.260e-14
Identity = 77/273 (28.21%), Postives = 109/273 (39.93%), Query Frame = 0

Query:  133 VLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACIL-CDPLLREHCRYRFSPETPASSACKGRGIEGRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWA----DDVGGGGS 400
            V  C  C     +  +K+  G  L C G+P+CK  ++F  +V EV   D+  C  CR             V +L+F+F +  +PPG P  +  CI  CD  LRE    ++            RG+ G      V   +      +P+  P  +S             R ENRPP    +P+            W+ + +     G              C+CGQ A +L VRKEGPN G++FY C       G C FF WA    D +G  GS
Sbjct:  654 VRKCPQCGRDMVLKKKKDSAGKFLSCMGYPSCKAAVWFPDSVLEVS-RDDSICPTCRPHP----------VNMLKFKFKRGSLPPGMPLEFVGCIGGCDETLREVLNLKYL-----------RGVGGDSS-TQVNPSSASRQSTRPVIDPALQS-------------RSENRPPPPNSTPA------------WSIQPSHSIQNGV------------VCNCGQDALLLTVRKEGPNQGRQFYKCNT-----GNCKFFLWAEQQNDQLGSAGS 861          

BLAST of EWM27003.1 vs. NCBI_GenBank
Match: gi|1042324603|ref|XP_017309956.1| (PREDICTED: DNA topoisomerase 3-alpha isoform X2 [Ictalurus punctatus])

HSP 1 Score: 86.2705 bits (212), Expect = 4.339e-14
Identity = 77/273 (28.21%), Postives = 109/273 (39.93%), Query Frame = 0

Query:  133 VLSCRGCKGQHSMTLRKNERGLMLGCQGFPACKGTIYFWGAVREVRVLDNRFCEECRKGGGGRGGVQKGQVALLQFQFYQRKVPPGTPEVYTACIL-CDPLLREHCRYRFSPETPASSACKGRGIEGRRGRGSVGAGAMEAAPFQPLPPPPFRSAVGEGAPGGAWRRRDENRPPILPLSPSSPMNGGGEKEGPWARKRARESDQGKGTRGMGGGGHAPSCHCGQAAKVLIVRKEGPNMGKEFYGCEIGQRAQGGCGFFQWA----DDVGGGGS 400
            V  C  C     +  +K+  G  L C G+P+CK  ++F  +V EV   D+  C  CR             V +L+F+F +  +PPG P  +  CI  CD  LRE    ++            RG+ G      V   +      +P+  P  +S             R ENRPP    +P+            W+ + +     G              C+CGQ A +L VRKEGPN G++FY C       G C FF WA    D +G  GS
Sbjct:  618 VRKCPQCGRDMVLKKKKDSAGKFLSCMGYPSCKAAVWFPDSVLEVS-RDDSICPTCRPHP----------VNMLKFKFKRGSLPPGMPLEFVGCIGGCDETLREVLNLKYL-----------RGVGGDSS-TQVNPSSASRQSTRPVIDPALQS-------------RSENRPPPPNSTPA------------WSIQPSHSIQNGV------------VCNCGQDALLLTVRKEGPNQGRQFYKCNT-----GNCKFFLWAEQQNDQLGSAGS 825