EWM30300.1, cds316 (CDS) Nannochloropsis gaditana

BLAST of EWM30300.1 vs. NCBI_GenBank
Match: gi|585112959|gb|EWM30300.1| (dna ligase [Nannochloropsis gaditana])

HSP 1 Score: 1967.97 bits (5097), Expect = 0.000e+0
Identity = 974/974 (100.00%), Postives = 974/974 (100.00%), Query Frame = 0

Query:    1 MMWMRSSRAWCSCRGRGRIKFAAFLPLRPLMTSPNRHNLLPHESAFPPLFVQPTPSEALTTAENKRKSLMSMSEVQFGRVRRRGKMAEGAVKPGQHRRKKLENLGVPDEVEQELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPATQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARRDVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEEDQVAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASREAAKKERRARGRSSSEGSGGRAPYAENAALGKPGDTMALHDGSDEVLLEERAGWGEKSVTALFSAIAARRKIPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAMQALEQGEVEEGRCGVEDRKDMEEHLNGGGVETREGEKNLPSKQVATAQDAGGMDTIEGIGPTVIASLRAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTLVDRASKGVI 974
            MMWMRSSRAWCSCRGRGRIKFAAFLPLRPLMTSPNRHNLLPHESAFPPLFVQPTPSEALTTAENKRKSLMSMSEVQFGRVRRRGKMAEGAVKPGQHRRKKLENLGVPDEVEQELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPATQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARRDVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEEDQVAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASREAAKKERRARGRSSSEGSGGRAPYAENAALGKPGDTMALHDGSDEVLLEERAGWGEKSVTALFSAIAARRKIPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAMQALEQGEVEEGRCGVEDRKDMEEHLNGGGVETREGEKNLPSKQVATAQDAGGMDTIEGIGPTVIASLRAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTLVDRASKGVI
Sbjct:    1 MMWMRSSRAWCSCRGRGRIKFAAFLPLRPLMTSPNRHNLLPHESAFPPLFVQPTPSEALTTAENKRKSLMSMSEVQFGRVRRRGKMAEGAVKPGQHRRKKLENLGVPDEVEQELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPATQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARRDVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEEDQVAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASREAAKKERRARGRSSSEGSGGRAPYAENAALGKPGDTMALHDGSDEVLLEERAGWGEKSVTALFSAIAARRKIPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAMQALEQGEVEEGRCGVEDRKDMEEHLNGGGVETREGEKNLPSKQVATAQDAGGMDTIEGIGPTVIASLRAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTLVDRASKGVI 974          

BLAST of EWM30300.1 vs. NCBI_GenBank
Match: gi|655965075|ref|WP_029007726.1| (DNA ligase (NAD(+)) LigA [Azospirillum halopraeferens])

HSP 1 Score: 503.056 bits (1294), Expect = 2.669e-161
Identity = 348/930 (37.42%), Postives = 473/930 (50.86%), Query Frame = 0

Query:   86 MAEGAVKPGQHRRKKLENLGVPDEVEQELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPATQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARRDVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEEDQVAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASREAAKKERRARGRSSSEGSGGRAPYAENAALGKPGDTMALHDGSDEVLLEERAGWGEKSVTALFSAIAARRK-IPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAMQA------------------LEQGEVEEGRCGVEDRKDMEE---HLNGGGVETREGEKNLPSKQV------ATAQDAG-------GMDT---------------IEGIGPTVIASLRAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTL 965
            M++ A  P   R   +++L + ++ + EL  LA E+  HDALY+ Q+ P ITDA+YD L RR  A+E   P L R         S   RVG++P               A F  V+H  PMLSL NAF+ EEVA +  R++R L           +  D+A                          +   AE K+DG+S SLRYEDG LV  ATRG+G  GEDVT NV +I  +P  LPPP      P  +EVRGEVY+ R  F  +N AR  +  P  +NPRNAA+GSLRQLD ++T +R L FF Y +  T +       +  TQ G+   L+ WGF +  P A    ++AL  ++  +   R++L FDIDGVVYK++ +  + R G  +RAPRWAIAHKF   +A TR++A+ +QVGRTG LTPVA LEPVTVGGVV++RA+LHN  ++AR+D+RVGDLV V+RAGDVIPQV+G   + L+ +  + E   +             P  CP C     +E D+    C  GL CPAQ+ ERL HFVSR AFDI+GLG + ++E R+  +V +P D+F+L       K+R  RG                                 E+ L  R GW EKSV  L  AI  RR  I L RFIFALG+ HVG+ TA+ +A H+GS+  W   M+A                  L    V+  R      +++E    H N   +      +NL  K++      A  +  G       G+ T               +  +G  V   + +F  ++RN   +E L R   V  E       D EV          D P+ GKTV+FTG L  M R+E +A+A  +GA    ++SKK D +V GA  GSKA K R+LG+  L E +WL L
Sbjct:    1 MSDVAASPSDLRAIPVDDLTI-EQAQAELAVLAAEIARHDALYHQQDRPEITDADYDRLVRRNGAIEARFPELQRS-------DSPSLRVGAAP--------------AAGFGKVRHAMPMLSLGNAFAPEEVAEFVARVRRFL-----------SLPDNA-------------------------PLAFEAEPKVDGLSCSLRYEDGRLVLAATRGDGAEGEDVTANVRTIRDVPAELPPP-----FPAVLEVRGEVYMTRDDFLAMNRARAERDEPPFANPRNAAAGSLRQLDPSITAQRPLCFFGYALGETSE------RVADTQSGIRRRLKGWGFRLNRPAARLGGVEALLRYYAGIGRRRAALPFDIDGVVYKVDAIDLQERLGFVSRAPRWAIAHKFPAEQAQTRLKAIAIQVGRTGALTPVAELEPVTVGGVVVSRATLHNEDEIARKDIRVGDLVTVQRAGDVIPQVVG---VILSQRPHESESFVF-------------PDHCPECGSLAVREPDEAVRRCTGGLICPAQAKERLRHFVSRNAFDIEGLGEKIIEEFRNDGLVRSPADIFTL-------KDRVKRG---------------------------------EITLVGRPGWKEKSVANLLDAIEERRNAIALHRFIFALGIRHVGEVTAKTLARHYGSLDAWLEGMKAAAREMPKPEWAELRTLKGLGPASVDALRAWFAAPENLETVRFHANAPDLRPETALRNLGIKELKQPAITALVERYGTFAACLDGLRTAFAGAPGDAWTELVNVRDVGAIVAEEIASFFLEDRNLEVIEGL-RAAGVRVE-------DAEVRQAA------DSPVAGKTVVFTGTLTTMGRNEAKARAEALGAKVAGSVSKKTDYVVAGADAGSKAAKARELGVTVLSEEEWLAL 791          

BLAST of EWM30300.1 vs. NCBI_GenBank
Match: gi|1064018098|gb|ODT25915.1| (DNA ligase (NAD(+)) LigA [Hyphomicrobium sp. SCN 65-11])

HSP 1 Score: 477.248 bits (1227), Expect = 1.529e-151
Identity = 330/905 (36.46%), Postives = 460/905 (50.83%), Query Frame = 0

Query:  113 ELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPA-TQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARRDVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEEDQVAVY------CPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASREAAKKERRARGRSSSEGSGGRAPYAENAALGKPGDTMALHDGSDEVLLEERAGWGEKSVTALFSAIAARRKIPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAM--------------------------QALEQGEVEEGRCGVEDRKDMEEHLNGGGVE-TREGEKNLPSK---QVAT--------AQDAGGMDT-----IEGIGPTVIASLRAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTLVD 967
            E + L  E+  HDALYY Q+ P I+DAEYD L  RL A+E   P    +LA+     S  +RVG++PV +              F  V+H  PMLSL NAFS EEVA +  R++R L             +DD                    P+       V AE KIDG+S+S+RYE G LVR ATRG+G  GEDVT N+ ++  IPH L        +P  +E+RGE+YL    F  LN A+   G+ + +NPRNAA+GSLRQLD ++T +R L FFAY   A         +LPA TQ  V+     WG  +      C  ++ L AF+  +  +R+SL +DIDGVVYK++ L  + R G  +R+PRWA AHKF+  +A T ++ +++QVGRTG LTPVA L+PVTVGGVV++ A+LHN  ++ R+D+R+GD V V+RAGDVIPQV+              E   +GA      KP   P  CP C    +++E +          C  G  CPAQ+ ERL HFVSR AFDI+GLGG R++      ++  P D+F+LA R+AA                     ++N                    L ER G+GEKS+  LF AI ARR+I L RF+FALGV HVG+TTA+ +A  FG+   +   +                          + L Q     GR  + D       L   G++  R G     ++    VAT        A++  G D      + GIG  V  SL  F  +  N++ V  L  EIE+   +++  ++             V   + GKTV+FTG L+ M+R+E +A+A + GA    ++S K D ++ GA  GSK  K R+LG+  L E +WL L++
Sbjct:   22 EHKKLVAEIARHDALYYQQDAPAISDAEYDALRTRLTAIEAQFP----DLAAAE---SPTQRVGAAPVEA--------------FGKVRHEVPMLSLGNAFSSEEVAEFVERVRRFLA----------LRADD--------------------PLV------VTAEPKIDGLSISIRYEGGQLVRAATRGDGIEGEDVTTNIRTVREIPHAL----KGAGVPDLIEIRGEIYLGTEDFRRLNAAQADAGSKVFANPRNAAAGSLRQLDASITAKRPLRFFAYTWGAAS-------ALPAKTQFDVVHAFAKWGLPINPRTVRCSSVEELIAFYDTVAIDRASLGYDIDGVVYKVDRLDYQERLGFVSRSPRWATAHKFAAEQATTVLQDIEIQVGRTGALTPVAKLQPVTVGGVVVSNATLHNEDEIRRKDIRIGDTVIVQRAGDVIPQVV----------SVIAERRPHGA------KPFKFPHTCPVCGSHAARDEGEEGGEAEAVRRCTGGFICPAQAKERLKHFVSRLAFDIEGLGGERIELFYDEGLIRAPADIFTLARRDAA---------------------SDNP-------------------LAERKGFGEKSIQNLFRAIDARRRIGLERFLFALGVRHVGETTARDLARAFGTYETFRTQVDLASKARPGASYRRLVTAKGLGPKTAETLMQAIAARGREDLFDGAPETFELAIAGIKGVRTGVAEALARAFPDVATFIETARSAAREMPGDDYRAFAGLSGIGEVVADSLIDFFDEPHNQKVVADLLSEIEI---QAYVRQAT------------VASAVTGKTVVFTGTLEKMSRNEAKAKAERAGAKVASSVSNKTDYVIAGAEAGSKLDKARELGVTVLNEDEWLALIE 787          

BLAST of EWM30300.1 vs. NCBI_GenBank
Match: gi|1113133756|gb|OJU08960.1| (DNA ligase (NAD(+)) LigA [Alphaproteobacteria bacterium 64-11])

HSP 1 Score: 468.003 bits (1203), Expect = 8.935e-149
Identity = 312/872 (35.78%), Postives = 442/872 (50.69%), Query Frame = 0

Query:  109 EVEQELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLP--PPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPA-TQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARRDVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEEDQ------VAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASR----EAAKKERRARGRSSSEGSGGRAPYAENAALGKPGDTMALHDGSDEVLLEERAGWGE-KSVTALFSAIAARRKIPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAMQALEQGEVEEGRCGVEDRKDMEEHLNGGGVETREGEKNLPSKQVATAQDAGGMDTIEGIGPTVIASLRAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTLV 966
            E  +EL  LA E+  HD  YY Q+ P I+DAEYD L RR   +E   P L+R         S   R+G+ P                +FA V+H  PMLSLDNAFS+E+VA +  R++R L  ++          DDA+                           V+AE KIDG+S SLRYE+G+ V+GATRG+G  GED+T N+ +I+ IP RL   PP         +EVRGEVY+  + F  LN+ ++ +G P+ +NPRN+A+GS+RQLD  +T  R L FFAY      D       +PA TQ G+L   E++GF V      C  L+ +  F+R +E  R+ L +DIDGVVYK++ L  + R G  +R+PRWAIAHKF   +A T + A+++QVGRTG L PVA L+PVTVGGVV+  A+LHN  ++AR D RVGD V ++RAGDVIPQ+L R  L    +                S+P   P +CP C     +E D+      V   C  GL C AQ++ERL +FV R  FDI+GLGG  ++      +++ P D+F+L  +     A   E RAR       + G+ P                         +++A  GE K V  L +AI  RR I L RFI ALG+ HVG+TTA+ +A H+ S+G +   M+A   G V+E                                          +++IEGIG  V  ++  F  +  N + ++ L + + V +                   P    P+ GKTV+FTG L+ MTR E +A+A  +GA    ++S K D++V G   GSK ++ +   ++ L+E  WL L+
Sbjct:   13 EAAKELDRLAREIAEHDRHYYLQDAPVISDAEYDALRRRNAEIEARFPRLIRP-------DSPSHRIGARP--------------SEKFAKVRHAVPMLSLDNAFSDEDVADFIGRVRRFLGLKD----------DDAVA--------------------------VMAEPKIDGLSASLRYENGIFVQGATRGDGEEGEDITANLRTISDIPLRLSGRPPKV-------LEVRGEVYMTHKAFAALNKRQEKEGKPVYANPRNSAAGSVRQLDPKITAGRALNFFAYAWGEISD-------MPADTQHGILQKFESYGFVVNPLVRRCETLEQVLKFYRDIEARRAKLGYDIDGVVYKVDRLDLQERLGLVSRSPRWAIAHKFPAEQAETVIEAIEIQVGRTGKLAPVARLKPVTVGGVVVQNATLHNEDEIARLDARVGDTVVIQRAGDVIPQIL-RVILEKRPRN---------------SRPYHFPHKCPVCGSHAVREVDEKTGREDVDRRCTGGLICDAQTVERLKYFVGRDQFDIEGLGGTLIELFHERGLLKEPADIFALTEKPKKVAAILAEHRARLSEERRAAEGKEPVKTKG--------------------KKKADEGEGKQVENLMAAIERRRTIGLDRFINALGIRHVGETTARLLARHYRSLGAFLAGMKA---GNVKE-----------------------------------------ELESIEGIGGVVAEAIFDFFDEPHNVKALDRLLKWLTVTD----------------MAAPANSSPVSGKTVVFTGTLEKMTRQEAKARAESLGAKVSGSVSAKTDIVVAGPGAGSKLKEAQKHNVQVLDEDAWLKLI 717          

BLAST of EWM30300.1 vs. NCBI_GenBank
Match: gi|757140733|ref|WP_042694966.1| (DNA ligase (NAD(+)) LigA [Azospirillum sp. B506])

HSP 1 Score: 469.929 bits (1208), Expect = 1.150e-148
Identity = 330/894 (36.91%), Postives = 443/894 (49.55%), Query Frame = 0

Query:  124 HDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPATQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARRDVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEEDQVAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASREAAKKERRARGRSSSEGSGGRAPYAENAALGKPGDTMALHDGSDEVLLEERAGWGEKSVTALFSAIAARRK-IPLSRFIFALGVTHVGQTTAQAIAAHFGSV---------------GNWWGAMQAL------------------EQGEVEEGRCGVEDRKDMEEHLNGGGVE---TREGEK------NLPSKQVATAQDAGG------MDTIE--GIGPTVIASLRAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTLV 966
            HD LY+ Q++P I+DA+YD L RR  A+E   P L R         S   RVG++P               A F  V+H  PMLSL NAF+ E+VA +  R++R L             SDDA                   P+T       VAE KIDG+S SLRYE G LV  ATRG+G  GE+VT NV +I  +P RLP      P P  +EVRGEVY+ R  F  +N AR  +G  L +NPRNAA+GSLRQLD A+T  R L FF Y +      G     +  TQ G+   L+ WGF++  P   C     L A++  +   R+ L FDIDGVVYK++ L+ ++R G  +RAPRWAIAHKF   +A TR++A+ +QVGRTG LTPVA LE +TVGGVV++RA+LHN  ++AR+D+RVGDLV V+RAGDVIPQV+    + L+ + AD             S P   P  CP C     +E  +V   C  GL C AQ+ ERL HFVSR AFDI+GLG + ++E      +++P D+F+L  R                                             V L  R GW EKSV  LF AI  RR  I L R I+ALG+ H+G+ TA+++A  + ++               G  W  + AL                  E G   +   G  D   +E  ++  G++   TR  +        L   + A  + AG       +D +    +G      L  F  +ERN   V  L   + VL  E                P   + P+ GKTV+FTG L+ MTRSE +A+A  +GA    ++S K D +V GA  GSKA K ++LG+E L E +WL  +
Sbjct:   38 HDRLYHQQDQPEISDADYDALVRRNLAIETRFPDLRRA-------DSPSLRVGAAP--------------AAGFGKVRHAIPMLSLGNAFAPEDVAEFDARVRRFL-----------GLSDDA-------------------PLT------FVAEPKIDGLSCSLRYEKGELVLAATRGDGAEGENVTANVRTIRDVPQRLP-----APFPDVLEVRGEVYMSRDDFLAMNSARAEKGEQLFANPRNAAAGSLRQLDPAITASRPLCFFGYAL------GELSEPIADTQWGIRERLKGWGFQLNRPAELCDGKDKLLAYYEGIGRRRAGLPFDIDGVVYKVDSLELQQRLGFVSRAPRWAIAHKFPAEQAQTRLKAITIQVGRTGALTPVAELEDITVGGVVVSRATLHNEDEIARKDIRVGDLVVVQRAGDVIPQVV---EVVLSQRPAD-------------SVPYVAPETCPVCGSLAIREAGEVVRRCTGGLICAAQAKERLRHFVSRDAFDIEGLGEKIIEEFWDEGFIKSPVDIFTLEGR---------------------------------------------VELIGRPGWKEKSVQNLFKAIQQRRDGIDLHRVIYALGIRHIGEVTAKSLARQYKTMQGWVDGMLAAEQAMPGQEWRDLHALSGVGPVTADTIIAWFADPESGSKLDFYAG-NDALRLETIISSLGIKRLNTRAAQALAERYGTLADWRAAMERAAGQAPGQAWLDLVATPDVGEVAAEELAGFFAEERNLVIVRGLMERLTVLEAEV---------------PKASNSPIAGKTVVFTGTLERMTRSEAKARAESLGAKVAGSVSGKTDYLVAGADAGSKAAKAKELGVEILTEDEWLARI 786          

BLAST of EWM30300.1 vs. NCBI_GenBank
Match: gi|1027699480|ref|WP_063634995.1| (DNA ligase (NAD(+)) LigA [Azospirillum humicireducens] >gi|1026295575|gb|ANC91914.1| DNA ligase (NAD(+)) LigA [Azospirillum humicireducens])

HSP 1 Score: 452.21 bits (1162), Expect = 7.383e-142
Identity = 329/921 (35.72%), Postives = 447/921 (48.53%), Query Frame = 0

Query:   93 PGQHRRKKLENLGVPDEVEQELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPATQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARRDVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEEDQVAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASRE-----------------AAKKERR-------------------------ARGRSSSEG--SGGRAPYAENAALGKPGDTMALHDGSDEVLLEERAGW-GEKSVTALFSAIAARRKIPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAMQALEQGEVEEGRCGVEDRKDMEEHLNGGGVETREGEKNLPSKQVATAQDAGGMDTIE--GIGPTVIASLRAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTLV 966
            P   R   +E L  PD+   +L  LA E+  HD LY+ Q++P I+DA+YD L RR  A+E   P L R                 SP      S  +G+   A F  V+H  PMLSL NAF+ E+VA +  R++R L             SDDA                   P+T       VAE KIDG+S SLRYE G LV  ATRG+G  GE+VT NV +I  +PHRLP      P P  +EVRGEVY+ R  F  +N AR  +G  L +NPRNAA+GSLRQLD ++T  R L FF Y +      G     +  TQ G+   L+ WGF++  P   C     L A++  +   R+ L FDIDGVVYK++ L+ ++R G  +RAPRWAIAHKF   +A TR++A+ +QVGRTG LTPVA LE +TVGGVV++RA+LHN  ++AR+D+RVGDLV V+RAGDVIPQV+    + L+ + AD             S P   P  CP C     +E  +V   C  GL C AQ+ ERL HFVSR AFDI+GLG + ++E      +++P D+F+L  R                   A  ERR                         AR   + +G   G RA  AE A  G+    +   +G   V  +    W  +    A     A    + L   I +LG+  +    AQA+A  +G++ +W  AM+                                        + VA A     +D +    +G      L  F  ++RN   V  L + + VL  E                P   + P+ GKTV+FTG L+ MTRSE +A+A  +GA    ++S K D +V GA  GSKA K ++LG+E L E +WL  +
Sbjct:    8 PPNPRSIDVEAL-TPDQAAADLAALAAEIAHHDRLYHQQDQPEISDADYDGLVRRNLAIETRFPELRR---------------ADSP------SLRIGAAPAAGFGKVRHAIPMLSLGNAFAAEDVAEFDARVRRFL-----------GLSDDA-------------------PLT------FVAEPKIDGLSCSLRYEKGELVLAATRGDGAEGENVTANVRTIRDVPHRLP-----APFPAVLEVRGEVYMNREDFLAMNAARAEKGEQLFANPRNAAAGSLRQLDPSITASRPLCFFGYAL------GEVSEPIAETQWGIRERLKGWGFQLNRPAELCDGKDKLLAYYDGIGRRRAGLPFDIDGVVYKVDSLELQQRLGFVSRAPRWAIAHKFPAEQAQTRLKAITIQVGRTGALTPVAELEDITVGGVVVSRATLHNEDEIARKDIRVGDLVVVQRAGDVIPQVV---EVVLSQRPAD-------------SVPYVAPETCPVCGSLAIREAGEVVRRCTGGLICAAQAKERLRHFVSRDAFDIEGLGEKIIEEFWDEGFIKSPADIFTLEGRVELIGRPGWKEKSVQNLFKAINERRDGIDLHRVIYALGIRHVGEVTAKSLARQYRTMQGWVDGMRA--AERAMPGQEWRDLHALNGVGPVTADTILAWFADAESAAKLDFYAGSEALRLETIIGSLGIKRLNTRAAQALATRYGTLADWRAAME----------------------------------------RAVAQAPGRPWLDLVATPDVGEVAAEELAGFFAEDRNLVIVGELAQRLTVLEAEV---------------PKASNSPIAGKTVVFTGTLERMTRSEAKARAESLGAKVAGSVSGKTDYLVAGADAGSKAAKAKELGVEILTEEEWLAKI 786          

BLAST of EWM30300.1 vs. NCBI_GenBank
Match: gi|1124690897|ref|WP_074969150.1| (DNA ligase (NAD(+)) LigA [Paracoccus aminovorans] >gi|1097674154|emb|SFH69253.1| DNA ligase (NAD+) [Paracoccus aminovorans])

HSP 1 Score: 451.44 bits (1160), Expect = 2.280e-141
Identity = 327/883 (37.03%), Postives = 448/883 (50.74%), Query Frame = 0

Query:  113 ELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPATQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARR-----------DVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEEDQVAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASREAAKKERRARGRSSSEGSGGRAPY-AENAALGKPGDTMALHDGSDEVL------LEERAGWGEKSVTALFSAIAARRKIPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAMQALEQGE--VEEGRCGVEDRKDMEEHLNGGGVETREGE-------KNLPSKQVATAQDAGGMDTIEGIGPTVIASLRAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTLVDR 968
            EL  L  +L+  +  YY  + P I+DAEYD   RRLEA+E   P    ELA           V  SP      ++ +G+    RFA V H  PMLSL+N FSE EV A+  R++  L             + DA+                         +   AE KIDG+SLSLRYE G LV+ ATRG+G VGE+VT N  +I  IP RL         P  +EVRGEVY+  + FE LN++    G  + +NPRNAA+GSLRQLD A+T  R L FFAY        G     L ATQ   +  L A GF+       CR +  + A    +E  R++L +DIDGVVYK+NDL  + R G  +  PRWA+AHKF    A TR+ A+D+QVGRTG L+PVA L PVTVGGVV++ A+LHN   +A R           D+R+GD V V RAGDVIP+V   A++ L  + AD             + P + P  CP C  P  +EE      C  GL CPAQ++E+L HFVSR AFDI+GLG ++++       +  P D+F+LA R+A   +  A   + + G   +A Y A   +LG     ++     D +       L E  GWGEKS  +LF AI  RRKIPL+R +FALG+ H+G+  AQ +A H+GS   W   + AL+         R   +  +         G   R  E       +  PS +   A    G+   +GIGP +  SL     +   +  ++ L   +  L  E+    S+I                 GKT++FTG L+ MTR+E +A+A  +GA    ++S K D+++ G   GSKA+K  DLGI  ++E +WL +  R
Sbjct:   32 ELARLTEKLDRANRAYYRDDAPFISDAEYDAGKRRLEAIEARFP----ELA-----------VQDSP------TAQVGAAPDGRFAKVPHRLPMLSLENGFSEAEVQAFVQRVRSFLN-----------LAPDAV-------------------------LECTAEPKIDGLSLSLRYEGGRLVQAATRGDGTVGENVTANARTIGDIPDRL----LGDDFPDVLEVRGEVYMSHQDFEKLNQS---DGGRIFANPRNAAAGSLRQLDPAVTAARPLRFFAYA------WGEISVPLAATQFEAVRRLGALGFQTNPLTRICRSVPEMMATWSEIEQMRATLGYDIDGVVYKVNDLGYQARLGMRSTTPRWALAHKFPAERAWTRLEAIDIQVGRTGALSPVARLHPVTVGGVVVSNATLHNEDYIAGRGADGSEIRGGKDIRIGDWVEVYRAGDVIPKV---ADVDLTQRPAD-------------ATPYAFPEVCPECGSPAIREEGDSVRRCTGGLICPAQAVEKLRHFVSRAAFDIEGLGAKQVELFHRLGWIAEPADIFTLARRDAEGNKLLAHA-ALATGEAAQAEYRALLLSLGVDALQLSAQSIPDVLARLSATPLAEIKGWGEKSAASLFQAIERRRKIPLARLLFALGIRHLGEVAAQDLARHYGS---WPALVAALDTARPAALAHRLAEDAAQAERAAALAEGRRARPSEARVAALAEAAPSPESMAAWT--GLIAGDGIGPVLAMSLSDSFANPAERAAIDRLVGFLTPLPPEARASDSEI----------------TGKTLVFTGTLEKMTRAEAKARAEALGAHVAGSVSAKTDLLIAGPGAGSKAKKAADLGIRVIDEDEWLAIAQR 806          

BLAST of EWM30300.1 vs. NCBI_GenBank
Match: gi|1134288410|ref|WP_076528762.1| (DNA ligase (NAD(+)) LigA [Gemmobacter megaterium] >gi|1131877672|emb|SIS66196.1| DNA ligase (NAD+) [Gemmobacter megaterium])

HSP 1 Score: 449.514 bits (1155), Expect = 8.376e-141
Identity = 325/901 (36.07%), Postives = 436/901 (48.39%), Query Frame = 0

Query:  109 EVEQELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPATQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARR-----------DVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEID-SRSKPISTPT----RCPSCAGPVSQEEDQVAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASRE----AAKKERRARGRSSSEGSGGRAPYAENAALGKPGDTMALHDGSDEVL-----------LEERAGWGEKSVTALFSAIAARRKIPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAMQALEQGEVEEGR------CGVEDRKDMEEHLNGGGVETREGEKNLPSKQVATAQDAGGMD--TIEGIGPTVIASL-RAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPR---VDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTLV 966
            E   E  +LA ++   +  Y+  + P I+DA YD L RRL+ LE A P L                       ST  +  +G      F  ++H  PMLSL+NAF + +VA +A R++R L  E+                                      G+   AE KIDG+SLSLRYE GVLV+ ATRG+G +GE+VT N  +IA IP +L         P  +EVRGEVY+    F  LN A+  +G  + +NPRNAA+GSLRQLD  +T  R L FFAY        G     L  TQ G +  L A GF+     A C     L A + R+E +R++L +DIDGVVYK+NDL  ++R G  A  PRWAIAHKF    A TR+  +D+QVGRTG ++PVA L PVTVGGVV++ A+LHN   +A R           D+R+GD V + RAGDVIP+V                     A++D SR  P +TP      CP C  P  +E    A  C  GL CPAQ++ERL HFVSR AFDI+GLG R+++       ++ P D+F+LA R+    A   + R R  +  +      P  +          + + D + E L           L    G+GEKS   LF+AI  RR I LSR IFALG+ HVG+  A+ +A HFGS    W A+       +   R            +            TR  EK      V     A   D   IEG+G  + ASL     RD   +  V+ L  ++           ++IE       PP       P+ G TV+FTG L+ MTRSE +A+A  +GA    ++S K   +V G   GSKA K  +LG+  L EA+WL L+
Sbjct:   13 EAAAEWGELATKIAQANTEYHRDDAPTISDAAYDGLKRRLQELEAAFPDLAD---------------------STSPTQAVGGPLAEGFGKLRHQVPMLSLENAFEDADVADFADRVRRFLGIED--------------------------------------GIAFTAEPKIDGLSLSLRYEKGVLVQAATRGDGEIGENVTANARTIADIPQQL------ADAPEELEVRGEVYMSHADFAALNAAQSQRGGKIFANPRNAAAGSLRQLDATITAARPLRFFAYAW------GALSAPLAPTQFGAIKRLAALGFQTNPLTALCTTPAELLAHYARIEAQRATLGYDIDGVVYKVNDLALQQRLGFRANTPRWAIAHKFPAELAWTRLEGIDIQVGRTGAMSPVARLSPVTVGGVVVSNATLHNEDYIAGRDSRGATIRGGKDIRIGDWVQIYRAGDVIPKV---------------------ADVDLSRRPPEATPYVFPDTCPECGSPAIREAGDSARRCTGGLVCPAQAVERLRHFVSRAAFDIEGLGARQVEFFYYLGKIDLPADIFTLAERDGGDAAMVVDPRRRLSNHDDVPEPWGPLHDQLVEILRASGVPVEDHNTEALIAGLRHLAGKPLSRVKGFGEKSTQNLFAAIEERRSIALSRLIFALGIRHVGEVAAKDLARHFGS----WSALADAVDMALPAARRHLLADEAEAAERAAAAAAGRRAEITRTREKVWADPPVPPEARAAWDDLLAIEGLGAVLAASLVTTLERDGPERAAVDRLIAQL-----------TEIE-------PPEARATASPVAGSTVVFTGTLERMTRSEAKARAESLGAKVAGSVSAKTTYLVAGPGAGSKATKAAELGVTVLTEAEWLELI 799          

BLAST of EWM30300.1 vs. NCBI_GenBank
Match: gi|494127363|ref|WP_007067135.1| (DNA ligase (NAD(+)) LigA [Fulvimarina pelagi] >gi|114540041|gb|EAU43161.1| DNA ligase, NAD-dependent [Fulvimarina pelagi HTCC2506])

HSP 1 Score: 449.129 bits (1154), Expect = 2.832e-140
Identity = 327/930 (35.16%), Postives = 452/930 (48.60%), Query Frame = 0

Query:  101 LENLGVPDEVE--QELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVFEDLNEARKAQGAPLLSNPRNAASGSLRQLDVALTRERRLGFFAYGI-EATEDTGVHPPSLPATQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVAR------------RDVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEED------QVAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASREAAKKERRARGRSSSEGSGGRAPYAENAALGKPGDTMALHDGSDEVLLEERAGWGEKSVTALFSAIAARRKIPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAMQALEQG------------------------EVEEGRCGVEDRKDMEEHLNGGGVETREGEKN------------LPSKQVATAQDAGGMDTIEGIGPTVIASLRAFVRDERNKRTVEALFRE-IEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTLVDRASKG 972
            +ENL   DE E   EL  LA E+  HD  YY  + P ++DA+YD L RR  A+E   P L+R         S  +RVG++P                 FA V+H  PMLSLDNAF+EE+VA +A R++R      +A+ + T                               G+ + AE KIDG+SLSLRY +G L   ATRG+G VGEDVT N  ++  IP R+         P   EVRGEVY+    F  LNE ++A G P  +NPRNAA+GSLRQ D  +TR R L FFAY   EA+E  G        TQ  V+A +  +GF V         + AL   +RR+E  R+SL +DIDGVVYK++DL+ ++  G  +R PRWAIAHKF+  +A T ++ +DV  GRTG L P+A LEPVTVGGVV++ A+LHN   +A              D+R+GD V ++RAGDVIPQ++      +A +   G            +KP   PT C  C     +E +          +C AGL+CPAQ  E L H VSR AFDI+GLG   ++ L    +V  P D+F+L   E  K     R  ++          A  A   +P           +V  ++RA    KS   +F  I ARR +PL RFIF LG+ +VG+TTA+A+A HF  V    G + A E+G                        ++++ +   E      +   G  V  R+  K             L +KQ A  Q    + T   IG     SL  F  ++ N++ V+AL +  +   NE      S+             D P+ G T++FTG+L+ MTR   +  A  +GA    ++S K D++V G   GSK  K  +LGIE ++E  W   + R   G
Sbjct:    6 VENL---DETEAASELERLAREIAEHDRAYYGDDAPTLSDADYDALRRRNTAIEARFPALIRA-------DSPSKRVGAAP--------------STTFAKVRHAVPMLSLDNAFTEEDVADFAKRVRR------IAKLQDT------------------------------DGLGITAEPKIDGLSLSLRYVNGELETAATRGDGTVGEDVTPNARTVDDIPVRIDRA------PKVFEVRGEVYMAHADFAALNERQEAAGKPRFANPRNAAAGSLRQKDAEVTRSRPLRFFAYAWGEASEVPG-------ETQSEVVAAIGKFGFPVNELMQRFETIDALIEHYRRIEENRASLGYDIDGVVYKVDDLRLQKDLGFVSRFPRWAIAHKFAAEKATTVLKGIDVTTGRTGALNPIARLEPVTVGGVVVSNATLHNEDYIAGIDADGETIRPSGLDIRIGDTVVIQRAGDVIPQIID----VVAEKRPAG------------AKPFVFPTHCAVCGSNAVREMNPRTGRPDSKRHCTAGLTCPAQGREGLKHLVSRSAFDIEGLGETLVEALFDEGLVRQPADIFTL-DFEKMKALLEERALAARREREAAEDEAARAEGREP-----------KVRGDDRAYDYTKSTQNIFDGIDARRTLPLERFIFGLGIPNVGETTAKALARHFSDVAALIGGVDAAEKGKPGSAWIELSALKGIGEKSFEQILDLDQSKLNEETYDPTRDPAAGLNVTRRKSLKERYGDASRIRAAILEAKQTAPTQAYLNLTTDSDIGTVATQSLINFFAEDHNRQAVDALLKAGVSTTNERMTAVASN-------------DSPVSGLTIVFTGSLEKMTREAAKEMAEGLGAKVSGSVSNKTDLVVAGPGAGSKLSKASELGIEVIDEDAWFKRIGRDPAG 821          

BLAST of EWM30300.1 vs. NCBI_GenBank
Match: gi|1131039216|ref|WP_075798770.1| (DNA ligase (NAD(+)) LigA [Roseomonas gilardii] >gi|1129030877|gb|APT57962.1| DNA ligase (NAD(+)) LigA [Roseomonas gilardii])

HSP 1 Score: 439.499 bits (1129), Expect = 7.690e-138
Identity = 309/874 (35.35%), Postives = 421/874 (48.17%), Query Frame = 0

Query:  108 DEVEQELRDLAHELEVHDALYYNQNEPCITDAEYDELARRLEALERAHPLLVRELASRTGYVSRGERVGSSPVFSTDASSDMGSGAGARFAPVKHGWPMLSLDNAFSEEEVAAWATRLQRALCEEEVAEGRQTATSDDAIGEGRSTARGGLAGGETERPVTGAGGVHVVAELKIDGVSLSLRYEDGVLVRGATRGNGFVGEDVTENVHSIASIPHRLPPPSTDCPLPPAVEVRGEVYLPRRVF----------EDLNEARKAQGAP------LLSNPRNAASGSLRQLDVALTRERRLGFFAYGIEATEDTGVHPPSLPATQVGVLALLEAWGFEVAHPWAPCRDLQALRAFHRRLEGERSSLAFDIDGVVYKLNDLQARRRAGRSARAPRWAIAHKFSPLEAVTRVRAVDVQVGRTGVLTPVAVLEPVTVGGVVITRASLHNFQDVARRDVRVGDLVAVRRAGDVIPQVLGRANLTLAAQGADGEDACYGAEIDSRSKPISTPTRCPSCAGPVSQEEDQVAVYCPAGLSCPAQSLERLAHFVSRGAFDIKGLGGRRLQELRSWEMVETPTDLFSLASREAAKKERRARGRSSSEGSGGRAPYAENAALGKPGDTMALHDGSDEVLLEERAGWGEKSVTALFSAIAARRKIPLSRFIFALGVTHVGQTTAQAIAAHFGSVGNWWGAMQALEQGEVEEGRCGVEDRKDMEEHLNGGGVETREGEKNLPSKQVATAQDAGGMDTIEGIGPTVIASLRAFVRDERNKRTVEALFREIEVLNEESFTERSDIEVSAGGRGPPRVDGPLVGKTVLFTGALQGMTRSEVEAQARQMGATPVKTLSKKVDMIVVGASPGSKARKGRDLGIEHLEEAQWLTL 965
            +E   EL  LA ++   D  Y+ Q+ P +TDA YD L RR  A+E   P L RE                SP     + +  G+   A F+  +HG PMLSLDN F   E A +A R++R L                           GL+  E  R          V E KIDG+S++L YEDG  VRGATRG+G VGED+T N+ ++  +P +LP      P P  +E+RGEV++ +  F           +  EAR+A G P      +  NPRNAA+GSLRQLD  +T  R L  FAY        G     +  T  G L  LE WGF+V        D  A  AF   L  +R+ LA+DIDGVVYKL+ L  + R G   RAPRWAIA KF   +A T +  + +QVGRTG LTP AV++PV VGGV++  A+LHN  ++AR+DVR+GD V ++RAGDVIPQ+ G                    +    S+P   P  CP+C     +   +V   C  GL CPAQ +ERL HF SR A DI+GLG   ++ L    +V TP D+F L     A  ER                             M   +G      +  +G   + +  L +AI ARR+  L RF+FALG+  +G   A+ +A H+G+V +W  AM       ++    G E R+D+     GG                           I+GIGP +   L  F  + RN   ++ L RE+     E+  E                 G L GK+++FTG+L+ MTR E EA+A  +GA  VK++SKK D++V+GA  GSKA+K  +L +  L EA+W  L
Sbjct:   16 EEARAELARLAGQIAEADLAYHQQDAPILTDAAYDALRRRNTAIEERFPALKRE---------------DSP-----SEASPGAAPAAGFSKSRHGTPMLSLDNVFGPGEFAEFAARIRRFL---------------------------GLSAEEVLR---------FVGEPKIDGLSVNLLYEDGRFVRGATRGDGTVGEDITRNLETLDELPRQLP-----APCPARIEIRGEVFMTKPDFLAFHAEQTRLNEEREARRAAGEPVGPAIRIPVNPRNAAAGSLRQLDPMVTASRPLRLFAYA------QGESSEPVAETHSGYLKRLEQWGFQVNPLSRLLPDEHAAEAFQAELAEKRAGLAYDIDGVVYKLDRLDWQSRLGFVGRAPRWAIAWKFPAEQATTTLLDIQIQVGRTGALTPRAVMQPVNVGGVMVQHATLHNEDEIARKDVRIGDTVILQRAGDVIPQITG----------------IVPEKRPKDSQPFVFPDTCPACGSHAVRPPGEVIKRCTGGLICPAQQVERLIHFASRNALDIEGLGIENIELLHREGLVNTPADIFRL----KAHTER-----------------------------MRGWEGWGGRGGKNPSGKDSRKIANLLAAIEARREPSLERFLFALGIRRIGAQNARLLARHYGTVEHWRQAM-------LDARVIGSEAREDL-----GG---------------------------IQGIGPAIAQELVEFFSEPRNLEALDDLLREVRPQPAEAVAE-----------------GALSGKSIVFTGSLETMTRQEAEARAESLGAKVVKSVSKKTDLVVIGADAGSKAKKAAELELRTLTEAEWREL 717