EWM27540.1, cds3143 (CDS) Nannochloropsis gaditana

BLAST of EWM27540.1 vs. NCBI_GenBank
Match: gi|585109807|gb|EWM27540.1| (zinc transporter [Nannochloropsis gaditana])

HSP 1 Score: 1187.56 bits (3071), Expect = 0.000e+0
Identity = 585/585 (100.00%), Postives = 585/585 (100.00%), Query Frame = 0

Query:    1 MGRGGQNGHNHKPDSATCAHGSGGLSGHTGHTHASAHEKQAQSQQKRADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPPIDGFLMMVVAIIGVVFNVVLMIIFGHEHGHGHGHSHGHSHGHSHGHSHGHSHGHSHMHENEEEGVEKGVGRRHVGQGVDVEAGKKRVWSHDSHGSQHGHTHQGGAVRFPSCDSVCSHKSQAGRGQKSRCSSASTSEEGTLLVDEEEEGKQGGMVLPMSTTYGSLSSSGVATTYGSLPSSGVAMQAHGSCHLNGHAHSDGEEEEEQGEDALTGSSTRLTARKLAAHEDPVKISRTRGKASTTKKEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHIKAADPDMAMHKAQAVFTQAGIDHITIQIQRDECLPNECAHPCVSVACVSTSPNSTSPGRGGCTYL 585
            MGRGGQNGHNHKPDSATCAHGSGGLSGHTGHTHASAHEKQAQSQQKRADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPPIDGFLMMVVAIIGVVFNVVLMIIFGHEHGHGHGHSHGHSHGHSHGHSHGHSHGHSHMHENEEEGVEKGVGRRHVGQGVDVEAGKKRVWSHDSHGSQHGHTHQGGAVRFPSCDSVCSHKSQAGRGQKSRCSSASTSEEGTLLVDEEEEGKQGGMVLPMSTTYGSLSSSGVATTYGSLPSSGVAMQAHGSCHLNGHAHSDGEEEEEQGEDALTGSSTRLTARKLAAHEDPVKISRTRGKASTTKKEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHIKAADPDMAMHKAQAVFTQAGIDHITIQIQRDECLPNECAHPCVSVACVSTSPNSTSPGRGGCTYL
Sbjct:    1 MGRGGQNGHNHKPDSATCAHGSGGLSGHTGHTHASAHEKQAQSQQKRADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPPIDGFLMMVVAIIGVVFNVVLMIIFGHEHGHGHGHSHGHSHGHSHGHSHGHSHGHSHMHENEEEGVEKGVGRRHVGQGVDVEAGKKRVWSHDSHGSQHGHTHQGGAVRFPSCDSVCSHKSQAGRGQKSRCSSASTSEEGTLLVDEEEEGKQGGMVLPMSTTYGSLSSSGVATTYGSLPSSGVAMQAHGSCHLNGHAHSDGEEEEEQGEDALTGSSTRLTARKLAAHEDPVKISRTRGKASTTKKEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHIKAADPDMAMHKAQAVFTQAGIDHITIQIQRDECLPNECAHPCVSVACVSTSPNSTSPGRGGCTYL 585          

BLAST of EWM27540.1 vs. NCBI_GenBank
Match: gi|1108986036|ref|XP_019126311.1| (PREDICTED: zinc transporter 8-like isoform X2 [Larimichthys crocea])

HSP 1 Score: 215.698 bits (548), Expect = 5.989e-59
Identity = 154/531 (29.00%), Postives = 271/531 (51.04%), Query Frame = 0

Query:   42 QSQQKRADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPPIDGFLMMVVAIIGVVFNVVLMIIFGHEHGHGHGHSHGHSHGHSHG--------HSHGHSHGHSHMHENEEEGVEKGVGRR-HVGQGVDVEAGKKRVWSHDSHG-SQHGHTHQGGAVRFPSCDSVCSHKSQAGRGQKSRCSSASTSEEGTLLVDEEEEGKQGGMVLPMSTTYGSLSSSGVATTYGSLPSSGVAMQAHGSCHLNGHAHSDGEEEEEQGEDALTGSSTRLTARKLAAHEDPVKISRTRGKASTTKKEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHI---KAADPDMAMHK-AQAVFTQAGIDHITIQIQRDECLPNEC 558
             +   R + + E+   R +L A ++  L+F+  EI GG  AGSLA++TDAAHL  D  +FI ++++  LS  P+T K +YG  RAE+L AL++   I ++   LVY  + R++         I+G +M++ +   V+ N+++ ++  H+ GHGH H    SHGHSHG        H    S+GHS+      +   KG+    H   G++          H + G + +GH+H+G      S + + S+   +G    +  S    +  G         G   G +     + G L+S+G   ++G L S+G    +HG  + NGH++ D    ++           +++    +  +  V + +    +S   + A+  N ++ AA +HV+ D  QS+ V ++ +II+FKP +++ DPICT +FSI +  + ++++     VL+EG P GV    + D LLA++GVT VH+LH+W L++ + +++ HI   ++ D    + +  QA F+      +TIQ++R   L   C
Sbjct:   44 DNNHARENRERERNASRKRLYAVSIICLIFIIGEIVGGYFAGSLAVMTDAAHLLVDFISFIISLLSLWLSSRPATHKLNYGWHRAEILGALLSVFTIWLVTGVLVYLAVKRLIT----NDYTIEGTVMLITSGCAVLANIIMALVL-HQSGHGHSHGGLSSHGHSHGGLSPNDKSHEGLSSNGHSNGGLRSSDHSHKGLSLNGHSNGGLNPNG-------HSNEGLNPNGHSHEGLNSNGHSNEDLTSNGHSSGGLNSNGHSHGGLNSNGHSHGGLNSNGHSNGDLSSNGHSSGGLNSNG--HSHGGLNSNG---HSHGGLNSNGHSNGDLSSNDQGHSHKKEEEHNQISNHAHSNDDLNVDLEQ---NSSAHGRRAQQANASVRAAFVHVVGDFFQSISVFISAIIIFFKPEYKMADPICTFLFSIFVLCTTITIMRDILIVLMEGTPAGVRYSEVRDGLLAVKGVTAVHNLHIWALTMNQAVLSAHIAIDESVDAQTVLREMTQACFSSYNFHSVTIQMERQADLKPGC 554          

BLAST of EWM27540.1 vs. NCBI_GenBank
Match: gi|1108986033|ref|XP_019126310.1| (PREDICTED: zinc transporter 8-like isoform X1 [Larimichthys crocea])

HSP 1 Score: 215.312 bits (547), Expect = 1.136e-58
Identity = 154/531 (29.00%), Postives = 271/531 (51.04%), Query Frame = 0

Query:   42 QSQQKRADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPPIDGFLMMVVAIIGVVFNVVLMIIFGHEHGHGHGHSHGHSHGHSHG--------HSHGHSHGHSHMHENEEEGVEKGVGRR-HVGQGVDVEAGKKRVWSHDSHG-SQHGHTHQGGAVRFPSCDSVCSHKSQAGRGQKSRCSSASTSEEGTLLVDEEEEGKQGGMVLPMSTTYGSLSSSGVATTYGSLPSSGVAMQAHGSCHLNGHAHSDGEEEEEQGEDALTGSSTRLTARKLAAHEDPVKISRTRGKASTTKKEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHI---KAADPDMAMHK-AQAVFTQAGIDHITIQIQRDECLPNEC 558
             +   R + + E+   R +L A ++  L+F+  EI GG  AGSLA++TDAAHL  D  +FI ++++  LS  P+T K +YG  RAE+L AL++   I ++   LVY  + R++         I+G +M++ +   V+ N+++ ++  H+ GHGH H    SHGHSHG        H    S+GHS+      +   KG+    H   G++          H + G + +GH+H+G      S + + S+   +G    +  S    +  G         G   G +     + G L+S+G   ++G L S+G    +HG  + NGH++ D    ++           +++    +  +  V + +    +S   + A+  N ++ AA +HV+ D  QS+ V ++ +II+FKP +++ DPICT +FSI +  + ++++     VL+EG P GV    + D LLA++GVT VH+LH+W L++ + +++ HI   ++ D    + +  QA F+      +TIQ++R   L   C
Sbjct:   60 DNNHARENRERERNASRKRLYAVSIICLIFIIGEIVGGYFAGSLAVMTDAAHLLVDFISFIISLLSLWLSSRPATHKLNYGWHRAEILGALLSVFTIWLVTGVLVYLAVKRLIT----NDYTIEGTVMLITSGCAVLANIIMALVL-HQSGHGHSHGGLSSHGHSHGGLSPNDKSHEGLSSNGHSNGGLRSSDHSHKGLSLNGHSNGGLNPNG-------HSNEGLNPNGHSHEGLNSNGHSNEDLTSNGHSSGGLNSNGHSHGGLNSNGHSHGGLNSNGHSNGDLSSNGHSSGGLNSNG--HSHGGLNSNG---HSHGGLNSNGHSNGDLSSNDQGHSHKKEEEHNQISNHAHSNDDLNVDLEQ---NSSAHGRRAQQANASVRAAFVHVVGDFFQSISVFISAIIIFFKPEYKMADPICTFLFSIFVLCTTITIMRDILIVLMEGTPAGVRYSEVRDGLLAVKGVTAVHNLHIWALTMNQAVLSAHIAIDESVDAQTVLREMTQACFSSYNFHSVTIQMERQADLKPGC 570          

BLAST of EWM27540.1 vs. NCBI_GenBank
Match: gi|808869316|gb|KKF19414.1| (Zinc transporter 8 [Larimichthys crocea])

HSP 1 Score: 212.231 bits (539), Expect = 2.180e-55
Identity = 156/542 (28.78%), Postives = 276/542 (50.92%), Query Frame = 0

Query:   31 HTHASAHEKQAQSQQKRADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPPIDGFLMMVVAIIGVVFNVVLMIIFGHEHGHGHGHSHGHSHGHSHG--------HSHGHSHGHSHMHENEEEGVEKGVGRR-HVGQGVDVEAGKKRVWSHDSHG-SQHGHTHQGGAVRFPSCDSVCSHKSQAGRGQKSRCSSASTSEEGTLLVDEEEEGKQGGMVLPMSTTYGSLSSSGVATTYGSLPSSGVAMQAHGSCHLNGHAHSDGEEEEEQGEDALTGSSTRLTARKLAAHEDPVKISRTRGKASTTKKEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHI---KAADPDMAMHK-AQAVFTQAGIDHITIQIQRDECLPNEC 558
            H H + H ++ + +++ A         R +L A ++  L+F+  EI GG  AGSLA++TDAAHL  D  +FI ++++  LS  P+T K +YG  RAE+L AL++   I ++   LVY  + R++         I+G +M++ +   V+ N+++ ++  H+ GHGH H    SHGHSHG        H    S+GHS+      +   KG+    H   G++          H + G + +GH+H+G      S + + S+   +G    +  S    +  G         G   G +     + G L+S+G   ++G L S+G    +HG  + NGH++ D    ++           +++    +  +  V + +    +S   + A+  N ++ AA +HV+ D  QS+ V ++ +II+FKP +++ DPICT +FSI +  + ++++     VL+EG P GV    + D LLA++GVT VH+LH+W L++ + +++ HI   ++ D    + +  QA F+      +TIQ++R   L   C
Sbjct:  470 HCHDNNHARENRERERNAS--------RKRLYAVSIICLIFIIGEIVGGYFAGSLAVMTDAAHLLVDFISFIISLLSLWLSSRPATHKLNYGWHRAEILGALLSVFTIWLVTGVLVYLAVKRLIT----NDYTIEGTVMLITSGCAVLANIIMALVL-HQSGHGHSHGGLSSHGHSHGGLSPNDKSHEGLSSNGHSNGGLRSSDHSHKGLSLNGHSNGGLNPNG-------HSNEGLNPNGHSHEGLNSNGHSNEDLTSNGHSSGGLNSNGHSHGGLNSNGHSHGGLNSNGHSNGDLSSNGHSSGGLNSNG--HSHGGLNSNG---HSHGGLNSNGHSNGDLSSNDQGHSHKKEEEHNQISNHAHSNDDLNVDLEQ---NSSAHGRRAQQANASVRAAFVHVVGDFFQSISVFISAIIIFFKPEYKMADPICTFLFSIFVLCTTITIMRDILIVLMEGTPAGVRYSEVRDGLLAVKGVTAVHNLHIWALTMNQAVLSAHIAIDESVDAQTVLREMTQACFSSYNFHSVTIQMERQADLKPGC 983          

HSP 2 Score: 112.464 bits (280), Expect = 3.197e-22
Identity = 47/120 (39.17%), Postives = 81/120 (67.50%), Query Frame = 0

Query:  406 KEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHIKAADP 525
            + A+  N ++ AA +HV+ DL+QS+ V ++ +II+FKP +++ DPICT +FSI +  + ++++     VL+EG P GV    + D LLA++GVT VH+LH+W L++ + +++ HI    P
Sbjct:  244 RRAQQANASVRAAFVHVVGDLLQSISVLVSAIIIFFKPEYKMADPICTFLFSIFVLCTTITIMRDILIVLMEGTPAGVRYSEVRDGLLAVKGVTAVHNLHIWALTMNQAVLSAHIAIGTP 363          

HSP 3 Score: 92.0485 bits (227), Expect = 1.087e-15
Identity = 60/186 (32.26%), Postives = 104/186 (55.91%), Query Frame = 0

Query:    4 GGQNGHNHK---PDS-ATCAHGSGGLSGHTGHTHASAHEKQAQSQQKRADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPPIDGFLMMVVAIIGVVFNVVLMI 185
            GG+N +N     P+  A    G+GG+     H H ++H ++        D + E++  R +L   +V  ++FM  EI GG  AGSLA++TDAAHL  D  +FI ++++  LS  P+T K +YG  RAE+L AL++   I ++   LVY  + R+++        I+G +M++ +   V+ N+++ +
Sbjct:   15 GGRNTYNTAGSVPNQEALQIDGAGGIK----HCHDNSHAQE--------DREREKKVARKRLYVVSVICMIFMIGEILGGYFAGSLAVMTDAAHLLVDFTSFIISLLSLWLSSRPATHKLNYGWHRAEILGALLSVFTIWLVTGVLVYLAVERLIS----DDYTIEGTVMLITSGCAVLANIIMAL 184          

BLAST of EWM27540.1 vs. NCBI_GenBank
Match: gi|1049462550|emb|SBT82398.1| (cation diffusion facilitator family protein, putative [Plasmodium ovale])

HSP 1 Score: 166.392 bits (420), Expect = 3.362e-41
Identity = 125/480 (26.04%), Postives = 230/480 (47.92%), Query Frame = 0

Query:   54 QRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPPIDGFLMMVVAIIGVVFNVVLMIIFGHEHGHGHGHSHGHSHGHSHGHSHGHSHGHSHMHENEEEGVEKGVGRRHVGQGVDVEAGKKRVWSHDSHGSQHGHTHQGGAVRFPSCDSVCSHKSQAGRGQKSRCSSASTSEEGTLLVDEEEEGKQGGMVLPMSTTYGSLSSSGVATTYGSLPSSG---VAMQAHGSCHLNGHAHSDGEEEEEQGEDALTGSSTRLTARKL--------AAHEDPVKISRTRGKASTTKKEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHIKA 522
            Q++   KLI A++  ++FM +EI   I++ SL+++TDA+HLF D+ +F   + + ++S        S+G  RAE++ AL +   I  L+AY++Y  + R+          +DG++M V A +  + N+ +  +    H HG          HS G    H H H  + EN  +G  K    +++   + +            + S + H      +   SC S  +          +  +    +E G  +  +  +G++      ++ +   +++S V      +   G   V +  H   + + +A S G+ EE    D+L G         +        +AH+       +  +     +E  S  I+L+ A+LH I+DL+Q++GV +A +II++ P + + DPIC+I+F  ++  + +S++ +  +VL+EG P  ++   + + LL I GV DVHDLHVW LS+G+P +  HI A
Sbjct:   26 QKKATKKLIVASIICIIFMIIEIIAAIISNSLSLMTDASHLFCDLLSFGLNLFSIYVSTFEGNVDMSFGYHRAEIIGALFSIFFIWALSAYILYSAVFRLF-----DVQTVDGYIMFVTAFVSTLANIFIAFVLK-VHSHGFEFIGQKRCSHSDG---SHDHTHISIWENNPKGQSKTRKYKNINSDIILA----------DNDSSYNHNISIDKMNTSSC-SYVAFDYYENVNNANTSNYYKVNEGGGDITGDVSDGRRKETNNSLNLSKNKINTSHVGNN-EDVDGDGNFKVGINDHIISNEHAYAISKGDCEENVMCDSLDGHLVETNVYDMHDGSHGHQSAHQ------HSHDRQHHGNEEINS--ISLKTAYLHAISDLLQNIGVMIASLIIWYNPKYSITDPICSIIFCFIVFSTTISVIKEILNVLMEGTPVSINLIDIKNDLLKIPGVIDVHDLHVWSLSIGKPALACHIVA 476          

BLAST of EWM27540.1 vs. NCBI_GenBank
Match: gi|813165160|ref|XP_012203640.1| (hypothetical protein SPRG_08905 [Saprolegnia parasitica CBS 223.65] >gi|641531539|gb|KDO25606.1| hypothetical protein SPRG_08905 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 151.754 bits (382), Expect = 5.123e-37
Identity = 70/156 (44.87%), Postives = 106/156 (67.95%), Query Frame = 0

Query:  406 KEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVH-IKAADPDMAMHKAQAVFTQAGIDHITIQIQRD-ECLPNECA 559
            K+ K  N+N+EAA++H + D +QSLGV +AG +I+++P WQV DPI T +FSIL+  + V ++  S HVL+EG P+G +P  + D LLA+  V++ HDLH+W LS G P ++VH +   +PD A+H+AQ      GIDH TIQ++   +  P +C+
Sbjct:  224 KKKKLENLNIEAAYIHALGDFIQSLGVCVAGGLIWYEPKWQVADPIATFLFSILVLGTTVGIIKSSIHVLMEGTPDGYEPDMILDGLLALPAVSNAHDLHIWSLSAGLPSLSVHLVTEGNPDEALHQAQEYLISKGIDHTTIQVEHALKTYPRDCS 379          

HSP 2 Score: 112.464 bits (280), Expect = 3.163e-23
Identity = 65/159 (40.88%), Postives = 92/159 (57.86%), Query Frame = 0

Query:   34 ASAHEKQAQSQQKRADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPP-----IDGFLMMVVAIIGVVFNVVLMIIF 187
            A+ +E       K A       R + KL  A +FALVFM  E+ GG +AGSLAI+TDAAHL +DVA F+ ++ A  +   P+++K S+G  RAE+L A+I+  VI +L  +LVY  + R    FQ          +DG LM ++A IG+V N+ LM I 
Sbjct:    2 AAMNEASPLVPAKSAPLNEGALRAKTKLQWACLFALVFMFAEVVGGFMAGSLAIMTDAAHLLSDVAGFLISLFAIWIQTFPASSKMSFGFHRAEILGAVISVLVIWVLTGFLVYAAVER----FQDAISDNPKEHVDGKLMFIIACIGLVVNLALMQIL 156          

BLAST of EWM27540.1 vs. NCBI_GenBank
Match: gi|669146258|ref|XP_008608691.1| (hypothetical protein SDRG_04785 [Saprolegnia diclina VS20] >gi|530739516|gb|EQC37758.1| hypothetical protein SDRG_04785 [Saprolegnia diclina VS20])

HSP 1 Score: 151.369 bits (381), Expect = 8.620e-37
Identity = 71/160 (44.38%), Postives = 108/160 (67.50%), Query Frame = 0

Query:  402 STTKKEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVH-IKAADPDMAMHKAQAVFTQAGIDHITIQIQRD-ECLPNECA 559
            + TKK  K  N+N+EAA++H + D +QSLGV LAG +I+++P WQV DPI T +FSIL+  + + ++  S HVL+EG P+G DP  + + LLA+  V++ HDLH+W LS G P ++VH +   +PD A+H+AQ      G+DH TIQ++   +  P +C+
Sbjct:  225 APTKK--KLENLNIEAAYIHALGDFIQSLGVCLAGGLIWYEPKWQVADPIATFLFSILVLGTTIGIIKSSIHVLMEGTPDGYDPDDILEGLLALPAVSNAHDLHIWSLSAGLPSLSVHLVSEGNPDEALHQAQEYLISKGVDHTTIQVEHALKTYPRDCS 382          

HSP 2 Score: 115.931 bits (289), Expect = 2.066e-24
Identity = 66/159 (41.51%), Postives = 93/159 (58.49%), Query Frame = 0

Query:   34 ASAHEKQAQSQQKRADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPP-----IDGFLMMVVAIIGVVFNVVLMIIF 187
            A+ +E       K A       R + KL  A +FALVFM  E+ GG +AGSLAI+TDAAHL +DVA F+ ++ A  +   P+++K SYG  RAE+L A+++  VI +L  +LVY  I R    FQ          +DG LM ++A+IG+V N+ LM I 
Sbjct:    2 AAMNEASPLVPAKSAPLNEGALRAKKKLQWACLFALVFMFAEVVGGFMAGSLAIMTDAAHLLSDVAGFLISLFAIWIQTFPASSKMSYGFHRAEILGAIVSVLVIWVLTGFLVYAAIDR----FQDAISDNPKEHVDGKLMFIIAVIGLVVNLALMKIL 156          

BLAST of EWM27540.1 vs. NCBI_GenBank
Match: gi|325185259|emb|CCA19747.1| (zinc transporter putative [Albugo laibachii Nc14])

HSP 1 Score: 146.362 bits (368), Expect = 8.151e-35
Identity = 70/183 (38.25%), Postives = 111/183 (60.66%), Query Frame = 0

Query:  393 KISRTRGKASTTKKEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHIKAADPDMAMHKAQAVFTQAGIDHITIQIQR-DECLPNECAHPCVSVACVSTSPNS 574
            ++  T  +  + K+     N+N+EAA++H + D +QS+GV +AG +I++ P WQ+ DPI T +FSI++  +   ++  S HVL+EG PEG+D   +   L A E V  +HDLH+W LS G P ++VH+ + DPD A+H+AQ      GI H TIQ+++  +  P  CA     + C   SP++
Sbjct:  245 QLDETLTEVKSKKRLITLENLNIEAAYIHALGDFIQSIGVCIAGGLIWYNPTWQIADPIATFIFSIVVLGTTFGIVTSSLHVLMEGTPEGIDHKEIELGLRACESVYGLHDLHIWSLSAGLPSLSVHLISDDPDNALHEAQNYLLSMGITHTTIQVEKASKRYPRNCAS---DLKCGQNSPHT 424          

HSP 2 Score: 111.309 bits (277), Expect = 1.103e-22
Identity = 62/144 (43.06%), Postives = 88/144 (61.11%), Query Frame = 0

Query:   45 QKRADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQG-TAPPIDGFLMMVVAIIGVVFNVVLMIIF 187
             +R        R + KL  A VF+++FM  E+ GGILAGSLAI+TDAAHL +DVA F  ++ A  +S  P++ K S+G +RAEV+ A+ +  VI +L   LVY  I R++   Q      ++G LM +VA IG+  N+VLM I 
Sbjct:   41 MRRIALDESSLRAKRKLQLACVFSILFMLAEVCGGILAGSLAIMTDAAHLLSDVAGFCISLFAIWVSTLPASAKLSFGFLRAEVIGAITSVLVIWVLTGVLVYAAIERLMDCLQSHPTEHVNGKLMFIVACIGLGVNLVLMRIL 184          

BLAST of EWM27540.1 vs. NCBI_GenBank
Match: gi|971506659|dbj|GAQ91546.1| (Zn2+ transporter [Klebsormidium nitens])

HSP 1 Score: 139.428 bits (350), Expect = 1.521e-31
Identity = 71/201 (35.32%), Postives = 117/201 (58.21%), Query Frame = 0

Query:  355 NGHAHSDGEEEEEQGEDALTGSSTRLTARKLAAHEDPVKISRTRGKASTTKKEAK-SRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHIK----AADPDMAMHKAQAVFTQAGIDHITIQIQR 550
            NG+ H  G E E +         TRL   +L   E      R   +  ++    K S NIN+  A+LHV+ DLVQS+GV LAG++I+F P WQ+VD +CT++FS+L+  + V++L     VLLE  P  ++   L ++L+ ++ V DVHDLH+W +++G+ L+  H++    A   D+ +   +    + GI+H+TIQ+++
Sbjct:  371 NGNGHGSGSELEYE---------TRLPIEELLNLEPHPSDGRGLVRRLSSHTNVKPSENINIRGAYLHVLGDLVQSVGVMLAGLVIWFFPEWQIVDLVCTLLFSVLVLATTVNILRDILEVLLESTPRAINAEELEERLMRVQSVVDVHDLHIWQITMGKTLLACHLRIDVDADSEDVLLEVTELCERKYGIEHVTIQVEK 562          

BLAST of EWM27540.1 vs. NCBI_GenBank
Match: gi|1190984187|ref|XP_020880826.1| (metal tolerance protein A1 [Arabidopsis lyrata subsp. lyrata])

HSP 1 Score: 133.265 bits (334), Expect = 7.048e-31
Identity = 60/150 (40.00%), Postives = 99/150 (66.00%), Query Frame = 0

Query:  405 KKEAKSRNINLEAAHLHVITDLVQSLGVALAGVIIYFKPHWQVVDPICTIVFSILICYSVVSMLAKSTHVLLEGVPEGVDPHALHDKLLAIEGVTDVHDLHVWMLSVGRPLVTVHIKA---ADPDMAMHKA-QAVFTQAGIDHITIQIQR 550
            +K  + +NIN++ A+LHV+ DL+QS+GV + G +I++ P W+V+D ICT+VFS+++  + + ML     VL+E  P  +D   L   L+ IE V DVH+LH+W ++VG+ L + H+K    AD +M ++K    ++ +  I H+TIQI+R
Sbjct:  195 EKSKEIKNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLVFSVIVLGTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHIWAITVGKALFSCHVKVRPEADDEMVLNKVIDYIWREYRISHVTIQIER 344          

HSP 2 Score: 110.923 bits (276), Expect = 5.655e-23
Identity = 82/183 (44.81%), Postives = 115/183 (62.84%), Query Frame = 0

Query:   48 ADAKMEQRRVRAKLIAATVFALVFMSVEIAGGILAGSLAIVTDAAHLFTDVANFITAIMASHLSETPSTTKHSYGLVRAEVLSALINTGVIVMLAAYLVYEGIHRIVAWFQGTAPPIDGFLMMVVAIIGVVFNVVLMIIFGHEHGHGHGHSHGHSHGHSHGHSHGHSHGHSHMHENEEEGVEK 230
            +DAK     +R KL    V  L+FMS+E+ GGI A SLAI+ DAAHL TDV  F  ++++   S   +  + SYG  R E+L AL++  +I +L   LVYE + R+V   Q T   +DGF M++VA  G++ N++++++ GH+HGHGHGH H HSH H HGH  GHSH H    E  E+ +EK
Sbjct:   21 SDAKERAASMR-KLCFVVVSCLLFMSIEVVGGIKANSLAILADAAHLLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGALVSIQLIWLLTGILVYEAVTRLV---QETNDDVDGFFMVLVATFGLLVNIIMIVVLGHDHGHGHGHDHSHSHVHGHGHCRGHSHSHG---EKAEQLLEK 196