EWM27566.1, cds3086 (CDS) Nannochloropsis gaditana

Overview
NameEWM27566.1
Unique Namecds3086
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002460.1:700685..701752 -

Link to JBrowse

Properties
Property NameValue
Protein idEWM27566.1
Productprotein kinase
Orig transcript idgnl|cribi|Naga_100015g69.6382.mrna
GenePTK
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002460.1supercontigCM002460.1:700685..701752 -
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
GO:0016301kinase activity
GO:0000166nucleotide binding
GO:0003676nucleic acid binding
GO:0004672protein kinase activity
GO:0005524ATP binding
Vocabulary: Biological Process
TermDefinition
GO:0006464cellular protein modification process
GO:0006468protein phosphorylation
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR011009Kinase-like_dom
IPR008271Ser/Thr_kinase_AS
IPR000719Prot_kinase_dom
IPR002290Ser/Thr_dual-sp_kinase
Homology
BLAST of EWM27566.1 vs. NCBI_GenBank
Match: gi|585109833|gb|EWM27566.1| (protein kinase [Nannochloropsis gaditana])

HSP 1 Score: 741.495 bits (1913), Expect = 0.000e+0
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0
Query:    1 MLHKDLRHPNVVMFRGASWRDGRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELRPDVLDVFAESLPEVERSETGITKERLKEPEGVEVTDACKTCPLNKANQPPPGVSLAQAQAAASTPEHECPLAQHKEFKGVVAHAVTRAIVYDDFRPPIKGCPYQGLKTLIEKCWIPDVNDRPGFAAVVEALDGRVRREVSDYVRPNGGSTSSHSTGSTSCFTSGSIDLMMVRDGSRQGFRVASGDVARLRAPHGTNT 355
            MLHKDLRHPNVVMFRGASWRDGRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELRPDVLDVFAESLPEVERSETGITKERLKEPEGVEVTDACKTCPLNKANQPPPGVSLAQAQAAASTPEHECPLAQHKEFKGVVAHAVTRAIVYDDFRPPIKGCPYQGLKTLIEKCWIPDVNDRPGFAAVVEALDGRVRREVSDYVRPNGGSTSSHSTGSTSCFTSGSIDLMMVRDGSRQGFRVASGDVARLRAPHGTNT
Sbjct:    1 MLHKDLRHPNVVMFRGASWRDGRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELRPDVLDVFAESLPEVERSETGITKERLKEPEGVEVTDACKTCPLNKANQPPPGVSLAQAQAAASTPEHECPLAQHKEFKGVVAHAVTRAIVYDDFRPPIKGCPYQGLKTLIEKCWIPDVNDRPGFAAVVEALDGRVRREVSDYVRPNGGSTSSHSTGSTSCFTSGSIDLMMVRDGSRQGFRVASGDVARLRAPHGTNT 355          
BLAST of EWM27566.1 vs. NCBI_GenBank
Match: gi|553183847|ref|XP_005853979.1| (deah (asp-glu-ala-his) box polypeptide 36 [Nannochloropsis gaditana CCMP526] >gi|422295081|gb|EKU22380.1| deah (asp-glu-ala-his) box polypeptide 36 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 539.65 bits (1389), Expect = 5.411e-172
Identity = 256/257 (99.61%), Postives = 256/257 (99.61%), Query Frame = 0
Query:    1 MLHKDLRHPNVVMFRGASWRDGRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELRPDVLDVFAESLPEVERSETGITKERLKEPEGVEVTDACKTCPLNKANQPPPGVSLAQAQAAASTPEHECPLAQHKEFKGVVAHAVTRAIVYDDFRPPIK 257
            MLHKDLRHPNVVMFRGASWRDGRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELRPDVLDVFAESL EVERSETGITKERLKEPEGVEVTDACKTCPLNKANQPPPGVSLAQAQAAASTPEHECPLAQHKEFKGVVAHAVTRAIVYDDFRPPIK
Sbjct:  631 MLHKDLRHPNVVMFRGASWRDGRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELRPDVLDVFAESLSEVERSETGITKERLKEPEGVEVTDACKTCPLNKANQPPPGVSLAQAQAAASTPEHECPLAQHKEFKGVVAHAVTRAIVYDDFRPPIK 887          
BLAST of EWM27566.1 vs. NCBI_GenBank
Match: gi|299116696|emb|CBN74841.1| (Tyrosine kinase specific for activated (GTP-bound) p21cdc42Hs [Ectocarpus siliculosus])

HSP 1 Score: 204.912 bits (520), Expect = 4.193e-55
Identity = 118/322 (36.65%), Postives = 165/322 (51.24%), Query Frame = 0
Query:    1 MLHKDLRHPNVVMFRGASWRDGRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELRPDVLDVFAESLPEVERSETGITKERLKEPEGVEVTDACKTCPLNKANQPPPGVSLAQAQAAASTPEHECPLAQHKEFKGVVAHAVTRAIVYDDFRPPIKGCPYQGLKTLIEKCWIPDVNDRPGFAAVVEALDGRVRREVSDYVRPNGGSTSSHSTGSTSCFTSG 322
            ++ KD  HPN+V    A+WR  RL+M+++Y  RG LA VL  +   L W   K  M L IA GMA+LH+  +++  ++S   C++HRDLKPQN+LVT  Y V+I+DFG A    ++   TQVGT L++APE+VRG+ Y E+CD+YSFAVVLLAML+L+ DV+ VFAE                                 + + + PP GV                         G+   A+T  IV +  RP +    +Q L+ LIE+CW P    RP F  +VE L+   R E+ +     G    + STG  S    G
Sbjct:  940 LIMKDFNHPNIVRLLRATWRGPRLMMILEYVSRGNLAKVLEETP-VLSWPRHKFRMCLDIANGMAYLHRFTHFNELSNSMHDCIIHRDLKPQNLLVTHDYRVKISDFGAATGRGNDPRDTQVGTLLYIAPEIVRGDCYDERCDIYSFAVVLLAMLQLKDDVVTVFAEEA-------------------------------VRRGSTPPAGVGWVGG--------------------GLTHMAITNQIVNEGLRPTLPSEVFQTLRILIEQCWAPIPVMRPTFEEIVEFLETIARDEIFERQPSEGEPELNESTGMKSGAVPG 1209          
BLAST of EWM27566.1 vs. NCBI_GenBank
Match: gi|698826215|ref|XP_009846136.1| (TKL/DRK protein kinase, variant [Aphanomyces astaci] >gi|574098694|gb|ETV64373.1| TKL/DRK protein kinase, variant [Aphanomyces astaci])

HSP 1 Score: 130.954 bits (328), Expect = 1.093e-31
Identity = 72/184 (39.13%), Postives = 106/184 (57.61%), Query Frame = 0
Query:    2 LHKDLRHPNVVMFRGASWRD-GRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELRPDVLDVFAESLPEVERSETGITKERL 184
            L  +LRHPN+V F GASW     +  + ++  RG L  VL+  +  L WK   L+MA+ I++GMA+LH          S    ++HRDLK  NILV+ S+  +I+DFG +R    ++TM+  GT L++ PE++R ERYSEK DVYSF +VL  +   R          +P  +   TG +K+R+
Sbjct:  156 LMMNLRHPNIVQFIGASWNSYSNICFVTEFLDRGDLYSVLKNPRIQLSWKQPLLSMAIDISRGMAYLH----------SMDPPIIHRDLKSMNILVSSSFGAKISDFGLSREKVLDDTMSVTGTPLWLPPEMIRAERYSEKADVYSFGIVLTELDTRR----------IPYHDIKNTGTSKKRV 319          
BLAST of EWM27566.1 vs. NCBI_GenBank
Match: gi|673035226|ref|XP_008871574.1| (TKL/DRK protein kinase, variant 1 [Aphanomyces invadans] >gi|574472794|gb|ETV99798.1| TKL/DRK protein kinase, variant 1 [Aphanomyces invadans])

HSP 1 Score: 130.568 bits (327), Expect = 1.706e-31
Identity = 66/151 (43.71%), Postives = 94/151 (62.25%), Query Frame = 0
Query:    2 LHKDLRHPNVVMFRGASWRD-GRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVL 151
            L  +LRHPN+V F GASW     +  + ++  RG L  VL+  +  L WK   L+MA+ I++GMA+LH          S    ++HRDLK  NILV+ S+  +I+DFG +R    ++TM+  GT L++ PE++R ERYSEK DVYSF +VL
Sbjct:  151 LMMNLRHPNIVQFIGASWNSYSNICFVTEFLDRGDLYSVLKNPRVQLSWKQPLLSMAIDISRGMAYLH----------SMDPPIIHRDLKSMNILVSSSFGAKISDFGLSREKVLDDTMSVTGTPLWLPPEMIRAERYSEKADVYSFGIVL 291          
BLAST of EWM27566.1 vs. NCBI_GenBank
Match: gi|673035224|ref|XP_008871573.1| (TKL/DRK protein kinase [Aphanomyces invadans] >gi|574472793|gb|ETV99797.1| TKL/DRK protein kinase [Aphanomyces invadans])

HSP 1 Score: 131.339 bits (329), Expect = 3.881e-31
Identity = 88/297 (29.63%), Postives = 134/297 (45.12%), Query Frame = 0
Query:    2 LHKDLRHPNVVMFRGASWRD-GRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELRPDVLDVFAESLPEVERSETGITKERLKEPEGVEVTDACKTCPLNKANQPPPGVSLAQAQAAASTPEHECPLAQHKEFKGVVAHAVTRAIVYDDFRPPIKGCPYQGLKTLIEKCWIPDVNDRPGFAAVVEALDGRVRREV 297
            L  +LRHPN+V F GASW     +  + ++  RG L  VL+  +  L WK   L+MA+ I++GMA+LH          S    ++HRDLK  NILV+ S+  +I+DFG +R    ++TM+  GT L++ PE++R ERYSEK DVYSF +VL  +   R          +P  +    G +K+R+                                  A ST  H                     + Y + RP +       ++ L  +C   D + RP F  ++  L+  V+RE+
Sbjct:  151 LMMNLRHPNIVQFIGASWNSYSNICFVTEFLDRGDLYSVLKNPRVQLSWKQPLLSMAIDISRGMAYLH----------SMDPPIIHRDLKSMNILVSSSFGAKISDFGLSREKVLDDTMSVTGTPLWLPPEMIRAERYSEKADVYSFGIVLTELDTRR----------IPYHDIKNVGTSKKRI----------------------------------AGSTLMH--------------------MVAYKNLRPNMSAKCLDSVRNLFVRCTSDDPDMRPTFEEIMVDLETNVKREI 373          
BLAST of EWM27566.1 vs. NCBI_GenBank
Match: gi|698826213|ref|XP_009846135.1| (TKL/DRK protein kinase [Aphanomyces astaci] >gi|574098693|gb|ETV64372.1| TKL/DRK protein kinase [Aphanomyces astaci])

HSP 1 Score: 131.339 bits (329), Expect = 3.994e-31
Identity = 89/297 (29.97%), Postives = 134/297 (45.12%), Query Frame = 0
Query:    2 LHKDLRHPNVVMFRGASWRD-GRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELRPDVLDVFAESLPEVERSETGITKERLKEPEGVEVTDACKTCPLNKANQPPPGVSLAQAQAAASTPEHECPLAQHKEFKGVVAHAVTRAIVYDDFRPPIKGCPYQGLKTLIEKCWIPDVNDRPGFAAVVEALDGRVRREV 297
            L  +LRHPN+V F GASW     +  + ++  RG L  VL+  +  L WK   L+MA+ I++GMA+LH          S    ++HRDLK  NILV+ S+  +I+DFG +R    ++TM+  GT L++ PE++R ERYSEK DVYSF +VL  +   R          +P  +   TG +K+R+                                  A ST  H                     + Y   RP +       ++ L  +C   D + RP F  ++  L+  V+RE+
Sbjct:  156 LMMNLRHPNIVQFIGASWNSYSNICFVTEFLDRGDLYSVLKNPRIQLSWKQPLLSMAIDISRGMAYLH----------SMDPPIIHRDLKSMNILVSSSFGAKISDFGLSREKVLDDTMSVTGTPLWLPPEMIRAERYSEKADVYSFGIVLTELDTRR----------IPYHDIKNTGTSKKRI----------------------------------AGSTLMH--------------------MVAYKQLRPNMSATCLDSVRDLFVRCTSDDPDMRPTFEDIMIDLETHVKREI 378          
BLAST of EWM27566.1 vs. NCBI_GenBank
Match: gi|673035228|ref|XP_008871575.1| (TKL/DRK protein kinase, variant 2 [Aphanomyces invadans] >gi|574472795|gb|ETV99799.1| TKL/DRK protein kinase, variant 2 [Aphanomyces invadans])

HSP 1 Score: 127.102 bits (318), Expect = 1.924e-30
Identity = 64/149 (42.95%), Postives = 92/149 (61.74%), Query Frame = 0
Query:    2 LHKDLRHPNVVMFRGASWRD-GRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAV 149
            L  +LRHPN+V F GASW     +  + ++  RG L  VL+  +  L WK   L+MA+ I++GMA+LH          S    ++HRDLK  NILV+ S+  +I+DFG +R    ++TM+  GT L++ PE++R ERYSEK DVYSF +
Sbjct:  151 LMMNLRHPNIVQFIGASWNSYSNICFVTEFLDRGDLYSVLKNPRVQLSWKQPLLSMAIDISRGMAYLH----------SMDPPIIHRDLKSMNILVSSSFGAKISDFGLSREKVLDDTMSVTGTPLWLPPEMIRAERYSEKADVYSFGI 289          
BLAST of EWM27566.1 vs. NCBI_GenBank
Match: gi|695388955|ref|XP_009519312.1| (hypothetical protein PHYSODRAFT_556648 [Phytophthora sojae] >gi|348684209|gb|EGZ24024.1| hypothetical protein PHYSODRAFT_556648 [Phytophthora sojae])

HSP 1 Score: 125.946 bits (315), Expect = 3.456e-29
Identity = 63/158 (39.87%), Postives = 95/158 (60.13%), Query Frame = 0
Query:    2 LHKDLRHPNVVMFRGASWRD-GRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAMLELR 158
            L  +LRHPN+V F GASW     +  + ++  RG L  VLR  +  + W    L M +  ++GMA+LH          S +  ++HRDLK  NILV+ ++  +++DFG +R    + TM+  GT L++ PE++RGERY+EK DVYSF + +LA L+ R
Sbjct:  149 LMMNLRHPNIVQFIGASWNSYSNICFVTEFLERGDLFAVLRNPENKMTWAKPILRMTIDTSRGMAYLH----------SMKPPIIHRDLKSMNILVSSTWGAKVSDFGLSREKSVDETMSVTGTPLWLPPEMIRGERYTEKADVYSFGIAVLAELDTR 296          
BLAST of EWM27566.1 vs. NCBI_GenBank
Match: gi|470434310|ref|XP_004338665.1| (serine/threonine kinase [Acanthamoeba castellanii str. Neff] >gi|440795532|gb|ELR16652.1| serine/threonine kinase [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 127.872 bits (320), Expect = 1.226e-28
Identity = 64/152 (42.11%), Postives = 96/152 (63.16%), Query Frame = 0
Query:    4 KDLRHPNVVMFRGASWRDGRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEARTVDHENTMTQVGTQLFVAPEVVRGERYSEKCDVYSFAVVLLAML 155
             ++RHPN+V+F GA  R   L ++ ++  +G+L  +L  S   L W+ V+L M    A+G+ +LH          + + C+VHRDLKP N+LV +S++V++ DFG AR  +   TMT+ GT  + APEV+RGE YSE  DVYSFA+++  ML
Sbjct: 1438 SEMRHPNIVLFIGACLRMPNLCLVTEWVKQGSLKALLGNSTIKLPWQ-VRLRMLRDAARGVHYLH----------TLEPCIVHRDLKPSNLLVDESWNVKVADFGFARIKEENATMTRCGTPAWTAPEVIRGEHYSESADVYSFALIMWEML 1578          

HSP 2 Score: 77.7962 bits (190), Expect = 7.812e-12
Identity = 46/155 (29.68%), Postives = 83/155 (53.55%), Query Frame = 0
Query:    6 LRHPNVVMFRGASWRDGRLLMLIDYAGRGTLADVLRRSKGTLRWKPVKLNMALGIAKGMAFLHQTRYYDMWTSSYQRCVVHRDLKPQNILVTDSYHVQITDFGEAR---TVDHENTMTQVGTQLFVAPEVVRGERYSEK--CDVYSFAVVLLAML 155
            LRHPNVV+F  A  R  R+ +++++   G+L D++          P+ + +AL  AKGM FLH +             ++HRDLK  N+L+   ++++++DFG  R    +  +    Q G+  ++APE++  E   +    DVY+F ++L  ++
Sbjct:  839 LRHPNVVLFMAACTRPPRMCIVMEFMALGSLYDLIHNELVPDIPLPLVVRLALQAAKGMHFLHSS------------GIIHRDLKSLNLLLDAKWNLKVSDFGLTRFKGDIKRDAQQQQQGSIHWLAPEILAEEPGIDYVLADVYAFGIILWELM 981          
The following BLAST results are available for this feature:
BLAST of EWM27566.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585109833|gb|EWM27566.1|0.000e+0100.00protein kinase [Nannochloropsis gaditana][more]
gi|553183847|ref|XP_005853979.1|5.411e-17299.61deah (asp-glu-ala-his) box polypeptide 36 [Nannoch... [more]
gi|299116696|emb|CBN74841.1|4.193e-5536.65Tyrosine kinase specific for activated (GTP-bound)... [more]
gi|698826215|ref|XP_009846136.1|1.093e-3139.13TKL/DRK protein kinase, variant [Aphanomyces astac... [more]
gi|673035226|ref|XP_008871574.1|1.706e-3143.71TKL/DRK protein kinase, variant 1 [Aphanomyces inv... [more]
gi|673035224|ref|XP_008871573.1|3.881e-3129.63TKL/DRK protein kinase [Aphanomyces invadans] >gi|... [more]
gi|698826213|ref|XP_009846135.1|3.994e-3129.97TKL/DRK protein kinase [Aphanomyces astaci] >gi|57... [more]
gi|673035228|ref|XP_008871575.1|1.924e-3042.95TKL/DRK protein kinase, variant 2 [Aphanomyces inv... [more]
gi|695388955|ref|XP_009519312.1|3.456e-2939.87hypothetical protein PHYSODRAFT_556648 [Phytophtho... [more]
gi|470434310|ref|XP_004338665.1|1.226e-2842.11serine/threonine kinase [Acanthamoeba castellanii ... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna3094rna3094Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications