EWM27690.1, cds2971 (CDS) Nannochloropsis gaditana

BLAST of EWM27690.1 vs. NCBI_GenBank
Match: gi|585109962|gb|EWM27690.1| (hydroxymethylpyrimidine kinase phosphomethylpyrimidine kinase thiamine-phosphate diphosphorylase [Nannochloropsis gaditana])

HSP 1 Score: 1187.94 bits (3072), Expect = 0.000e+0
Identity = 585/585 (100.00%), Postives = 585/585 (100.00%), Query Frame = 0

Query:    1 MVLYPRVHSCLLLVLASLALGSTAEAFVPATASRKPWRYAWPNTIMASSTTESGRSSEYDVAGGPQLPVPPIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAAVAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVTSRVEEGSKKRPGPFAECERDMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLVDVPLVAIGGIGLEAAQGVRDAGADGICVISALTQASDVAQAVQAWRAVLRTGK 585
            MVLYPRVHSCLLLVLASLALGSTAEAFVPATASRKPWRYAWPNTIMASSTTESGRSSEYDVAGGPQLPVPPIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAAVAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVTSRVEEGSKKRPGPFAECERDMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLVDVPLVAIGGIGLEAAQGVRDAGADGICVISALTQASDVAQAVQAWRAVLRTGK
Sbjct:    1 MVLYPRVHSCLLLVLASLALGSTAEAFVPATASRKPWRYAWPNTIMASSTTESGRSSEYDVAGGPQLPVPPIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAAVAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVTSRVEEGSKKRPGPFAECERDMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLVDVPLVAIGGIGLEAAQGVRDAGADGICVISALTQASDVAQAVQAWRAVLRTGK 585          

BLAST of EWM27690.1 vs. NCBI_GenBank
Match: gi|299472726|emb|CBN80294.1| (Thiamine monophosphate synthase [Ectocarpus siliculosus])

HSP 1 Score: 491.886 bits (1265), Expect = 3.306e-165
Identity = 286/536 (53.36%), Postives = 364/536 (67.91%), Query Frame = 0

Query:   51 TESGRSSEYDVAGGPQLPVPPIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAA--VAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVTSRVEEGSKKRPGPFAECERDMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKA-------NARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLVDVPLVAIGGIGLEAAQGVRDAGADGICVISALTQASDVAQAVQAW 577
            T S  +S   ++ G   P  PIVW+IAGSDSGGGAG+QADL A H+LG HGCSVITA+TAQNSH V  VE +S++M++ T+  L+ D+P  A+K+GM+ T  VV  VG FL     EG  G V+CDPVMV++SGS L  E A   M + +FPRAT+ITPN  EAE+L+G +LRTP D+E  A  LLA   +G VL+KGGH+ D    ER   G     A    +QDY  +       W+T+PR+++ +THGTGCTLSSA AA LA+ L   D+VVLAKAYV+QGIR AR+ G+GPGPV  TGWP+ A  FPWVT+  E G+  RP  F +C  D GLY  V +A WV++LL LGV+DIQLR+K      L+ EVA AA++CR +         RLWVND+W  AVR+ AYG+H+GQED++         L A+   G+RLG+STH++ EL RA A+RPSYISLGP+F TTSKKV F P+G   +  WR LVDVPL+AIGGI LE A  V +AGAD I VISA+T+A DV +AV+ W
Sbjct:   36 TRSQSTSRAAMSSGEGCP-KPIVWTIAGSDSGGGAGIQADLHAMHSLGVHGCSVITAMTAQNSHAVTHVEYASVEMIQATIDALQSDLPPAAVKLGMMGTDAVVSVVGAFL-----EGYKGNVVCDPVMVSTSGSRLLPEGAEALMKERVFPRATMITPNLIEAEVLLGRSLRTPADVEAGAASLLASSAAGGVLIKGGHSED----EREPGGADRPSARQRYSQDYWTDGTPGGSFWLTTPRVDSDDTHGTGCTLSSAAAACLAKGLDPADSVVLAKAYVTQGIRVARRFGKGPGPVAHTGWPSRADCFPWVTATAEGGAGGRPEAFPKCHDDWGLYPVVETAEWVEKLLSLGVRDIQLRVKGASPEALDAEVARAAQACRDSAAGAGGGGGRLWVNDFWRAAVRHGAYGVHLGQEDLEAGGGE---ALSAISKAGLRLGLSTHSYLELARATAVRPSYISLGPVFETTSKKVAFSPRGAVLVGAWRALVDVPLIAIGGISLERAPEVLEAGADSIAVISAITKADDVEEAVRQW 558          

BLAST of EWM27690.1 vs. NCBI_GenBank
Match: gi|551339251|ref|WP_022958671.1| (bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase [Spongiibacter tropicus])

HSP 1 Score: 413.305 bits (1061), Expect = 1.276e-135
Identity = 238/514 (46.30%), Postives = 325/514 (63.23%), Query Frame = 0

Query:   71 PIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHAL--DGLGGERSASGEGLFDAAVAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVTSRVEEGSKKRPGPFAECERDMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLVD--VPLVAIGGIGLEAAQGVRDAGADGICVISALTQASDVAQAVQAWRAV 580
            PI WSIAGSDSGGGAG+QADL  FH  G HGC+VITA+TAQNS  V  V V+S   L   +  L+ D+PA AIK+GML +A+VV  V  +L +       G V+CDPVMV++SG +L +    Q +++ + PRA L+TPN  EAE L+   + T E++ERAA D++ +G   +VL+ GGH +  DGL                  DY   ++G+ G W++ P ++  NTHGTGCTLSSA+AA LA+   + DA+VLAKAYVSQG+R + +LG GPGPV   GWP    D+P ++ R     K +P  F  C+ ++GLY  V S+ WV+RLL+ GVK IQLRIKD     L+ E+  A    R+  AR+++NDYW  A+   AYG+H+GQED+D         + A+ + G+RLGVSTH+  E+ RA ALRPSYI++GP++ TTSK++RFGPQG+ RLR W  L+     L AIGGI L+ A  V   G     +I+A+T+A D    V +  A+
Sbjct:    7 PIAWSIAGSDSGGGAGIQADLATFHDFGVHGCTVITAITAQNSFAVGHVAVTSHRALAAQINALDSDLPAGAIKLGMLGSAEVVEVVAKYLQDY-----PGPVVCDPVMVSTSGGLLMENGVSQVLVEKLLPRADLLTPNISEAETLLDRKITTAEEVERAAQDIVVLGAR-SVLLTGGHLVTRDGL----------------CHDYW--TDGEQGFWLSGPCIDTLNTHGTGCTLSSAIAALLARGYEMADALVLAKAYVSQGLRLSVQLGGGPGPVTHMGWPKQLCDYPGLSRRF---PKAKPA-FPACDGELGLYPVVDSSEWVERLLKSGVKTIQLRIKDPHQPNLDEEIQRAVALGREYQARVFINDYWPQAIAAGAYGVHLGQEDLDV------ADIQAIADAGLRLGVSTHSHYEVARAHALRPSYIAIGPVYPTTSKEMRFGPQGVNRLRHWVELLSPGYTLTAIGGINLKRADEVLATGVGSCAMITAITEADDPESVVASLMAL 486          

BLAST of EWM27690.1 vs. NCBI_GenBank
Match: gi|1084642887|gb|OGO91571.1| (hypothetical protein A3F41_02540 [Coxiella sp. RIFCSPHIGHO2_12_FULL_44_14])

HSP 1 Score: 409.068 bits (1050), Expect = 6.695e-134
Identity = 238/517 (46.03%), Postives = 315/517 (60.93%), Query Frame = 0

Query:   71 PIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAAVAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVTSRVEEGSK-KRPGPFAECERD-MGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLVDVPLVAIGGIGLEAAQGVRDAGADGICVISALTQASDVAQAV-------QAWR 578
            PI W+IAGSDSGGGAG+QADL  F A+G HGCSVITA+TAQNS  VR +       +   +A L DD+PA  IKIGML  A  + A+  +L     +   G VI DPVMV++SGSVL  +  +Q MI  +FPRA L+TPN  EAE +   ++RTP+D+E+AA  +L +G    VL+KGGH +D                    DY   ++G+   W+   RL + NTHG+GCTLS+A+AA+LAQ     DA+V++K YV+Q IR A+ LGRGPGPV    WP  + D P  T+ +    K      F  C +  +GLY  + S+ W+++LL LG+  IQLRIK++    LE E+  A +      ARL++NDYWELA+RY AYG+H+GQED+          + A+   G+RLG+STH+  E+ RA AL PSYI+ GPIF TTSK + F PQGL RL+ W+  +D P+VAIGGIGL     V   G  GI +ISA+T A D  Q V       Q WR
Sbjct:    5 PIAWTIAGSDSGGGAGIQADLHTFQAIGVHGCSVITAVTAQNSVGVRDIAYLPATHVAAQIAALHDDLPACVIKIGMLGNASAISAIVDYL-----QSFKGTVIVDPVMVSTSGSVLLPDDIKQLMIQRLFPRAGLLTPNLFEAEKITESSIRTPQDMEKAAQQILLLGPR-RVLLKGGHRIDN----------------DCNDYW--TDGRESFWLNQVRLPHANTHGSGCTLSAAIAAYLAQGYAWRDALVMSKIYVTQCIRHAKPLGRGPGPVTPGPWPLHSPDLP--TATIMPLMKVSTQNIFPSCGKTPLGLYPIIDSSDWIEKLLPLGITTIQLRIKNKTGKALEKEIQRAVQLAEHHQARLFINDYWELAIRYGAYGVHLGQEDLAHAN------INAIHRAGLRLGISTHSPYEIARARALCPSYIAFGPIFPTTSKAMSFPPQGLERLQYWQRCLDYPVVAIGGIGLSEFDAVLATGVSGIAMISAITLAPDPIQTVDKLLNKGQQWR 489          

BLAST of EWM27690.1 vs. NCBI_GenBank
Match: gi|822637200|ref|WP_047011026.1| (bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase [Spongiibacter sp. IMCC21906] >gi|822479350|gb|AKH68426.1| hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiamine-phosphate diphosphorylase [Spongiibacter sp. IMCC21906])

HSP 1 Score: 405.601 bits (1041), Expect = 1.462e-132
Identity = 233/501 (46.51%), Postives = 318/501 (63.47%), Query Frame = 0

Query:   71 PIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAAVAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVTSRVEEGSKKRPGPFAECERDMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLV--DVPLVAIGGIGLEAAQGVRDAGADGICVISALTQASD 569
            PIVWSIAGSDSGGGAG+QADL  FH  G HGC+VITA+TAQNS  V  V  +    L   +  L+ D+ A AIK+GML   DVV+ V  +L     E   G V+CDPVMV++SG  L +    + +I+ + PRA L+TPN  EAE L+   +   E++ERAA D++A+G   +VL+ GGH L     +R              DY   ++G +G W++   ++  NTHGTGCTLSSA+AA +A+D  L DA+VLAKAYVSQG+R + +LG GPGPV   GWP   +DFP V+ R+    K     F  C+  +GLY  V SA W+ RLL+ GVK IQLR+K+    +LE E+ EA    ++ NAR+++NDYW+ AV   AYG+H+GQED+D         L A+   G+RLGVSTH++ E+ RA  +RPSYI++GPI+ TTSK++ F PQG+A+L++W +++     L AIGGI ++ A  V   G     +ISA+TQA D
Sbjct:    7 PIVWSIAGSDSGGGAGIQADLATFHDFGVHGCTVITAITAQNSFTVGHVSTTPRRALAAQINALDSDLQADAIKLGMLGGVDVVKMVAKYL-----EDYPGFVVCDPVMVSTSGGELMESGVAEVLIEQLLPRADLLTPNLSEAEALLNRKIDGLEEVERAAADIVALGAR-SVLITGGH-LSARNNQR-------------HDYW--TDGLNGFWLSGQNIDTLNTHGTGCTLSSAIAALMARDFDLKDALVLAKAYVSQGLRMSVQLGGGPGPVAHVGWPQRLADFPGVSRRLPAAGKVL--AFPACDGPLGLYPVVDSAEWIARLLKCGVKTIQLRVKEVSGSDLEKEITEAVALGQQYNARVFINDYWQQAVAAGAYGVHLGQEDLD------GTDLHAIAEAGLRLGVSTHSYHEVARAHGIRPSYIAIGPIYPTTSKQMPFSPQGVAKLQRWVNILAPHYTLTAIGGIDVKRADAVLATGVGSAAMISAITQAED 477          

BLAST of EWM27690.1 vs. NCBI_GenBank
Match: gi|646612412|ref|WP_025563166.1| (bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase [Psychromonas sp. SP041])

HSP 1 Score: 399.053 bits (1024), Expect = 5.823e-130
Identity = 232/521 (44.53%), Postives = 323/521 (62.00%), Query Frame = 0

Query:   72 IVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLS-EKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAAVAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVT-------SRVEEGSKKRPGP-FAECER-DMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLV-DVPLVAIGGIGLEAAQGVRDAGADGICVISALTQASDVAQAVQAWRAVL 581
            IVW+IAGSDS  GAG+QADL  FH LG  GCSVITA+TAQNS  V+ VE  S  M    LA+L +DMPAK IKIG+LA+ + VR +   L+  K T      VI DPV V S+G  + +E   + +   + P+  L+TPN +E   L G+ L + E  + A   L++MG  G+VL+KGGH               L D  +A DY   S+GK  + +TSPRL+N +THGTGCTL+SA+AA +A D  + DA+V+AKAY+ QG++A++ LG G G V   GWP    DFP V          ++       GP F  C+   +GLY  V S  WV+R+L+LGVK +QLRIKD+   ++E ++ +A    ++ +ARL++NDYW+LA+++ AYG+H+GQEDMD      DLPL+A  + G+RLG+STH + E+ RA  L+PSYI+LG IF T +K +   PQGL RL ++ +L+ D+  VAIGGI LE A  V   G   I V++A+T A D  Q +  +  ++
Sbjct:    4 IVWTIAGSDSSAGAGIQADLYTFHDLGADGCSVITAVTAQNSQAVQLVESVSEKMFTQQLASLAEDMPAKVIKIGLLASVEHVRILAQQLALYKQTWSRKPFVIYDPVSVASTGDEMAEEGINEIIKTQLLPQIDLLTPNANEVLTLSGHALISGESFKPAVDKLVSMG-CGSVLIKGGHF-------------ELVD-DIALDYW--SDGKREIALTSPRLDNVHTHGTGCTLASAIAATMALDYFIEDALVIAKAYLYQGLKASKALGAGEGSVAHCGWPTQRDDFPEVVLPESSIGYELDLPGNLEAGPGFEPCDTIKLGLYPVVDSVEWVERVLKLGVKTLQLRIKDKQPEQVEQQIIDAIALGKQYDARLFINDYWQLAIKHNAYGVHLGQEDMD----VADLPLIA--DAGLRLGLSTHGYYEMLRARQLKPSYIALGHIFPTQTKDMPSDPQGLTRLSKYANLMSDMATVAIGGINLERAPAVWQTGIGSIAVVTAITLADDPKQVINQFNEIM 501          

BLAST of EWM27690.1 vs. NCBI_GenBank
Match: gi|655487142|ref|WP_028868693.1| (bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase [Psychromonas arctica])

HSP 1 Score: 395.201 bits (1014), Expect = 1.625e-128
Identity = 229/521 (43.95%), Postives = 325/521 (62.38%), Query Frame = 0

Query:   72 IVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLS-EKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAAVAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVT-------SRVEEGSKKRPGP-FAECER-DMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLV-DVPLVAIGGIGLEAAQGVRDAGADGICVISALTQASDVAQAVQAWRAVL 581
            IVW+IAGSDS  GAG+QADL  FH LG  GCSVITA+TAQNS  V+ VE  S  M    LA+L +DMPAK IKIG++A+ + V+ +   L+  K T      VI DPV V S+G  + +E   + +   + P+  L+TPN +E   L G+ L + E  + A   L++MG  G+VL+KGGH               L D  +A DY   S+G+  + +TSPRL+N +THGTGCTL+SA+AA +A D  + DA+V+AKAY++QG++A++ LG G G V   GWP   +DFP V          ++       GP F  C+   +GLY  V S  WV+R+L++GVK +QLRIKD+   ++E ++ +A    ++ +ARL++NDYW+LA+++ AYG+H+GQEDMD      DLPL+A  + G+RLG+STH + E+ RA  L+PSYI+LG IF T +K +   PQGL RL ++ +L+ D+  VAIGGI LE A  V   G   I V++A+T A D  Q V  +  ++
Sbjct:    4 IVWTIAGSDSSAGAGIQADLCTFHDLGADGCSVITAVTAQNSQAVQLVESVSEKMFAQQLASLAEDMPAKVIKIGLIASVEHVKILAQQLALYKQTWARKPFVIYDPVAVASTGDEMAEEGINEVIKTQLLPQIDLLTPNANEVLTLSGHALISGESFKPAVDKLISMG-CGSVLIKGGHF-------------ELVD-DIALDYW--SDGEREIALTSPRLDNVHTHGTGCTLASAIAATMALDYFIEDALVIAKAYLNQGLKASKALGAGEGSVAHCGWPTLRADFPEVVLPESSIGYELDLPGSLEAGPGFKPCDTIKLGLYPVVDSVEWVERVLKMGVKTLQLRIKDKQPEQVEQQIIDAIALGKQYDARLFINDYWQLAIKHNAYGVHLGQEDMD----VADLPLIA--DAGLRLGLSTHGYYEMLRARQLKPSYIALGHIFPTKTKDMPSDPQGLTRLSKYANLMSDMATVAIGGINLERAPAVWQTGIGSIAVVTAITLADDPKQVVNQFNEIM 501          

BLAST of EWM27690.1 vs. NCBI_GenBank
Match: gi|495527728|ref|WP_008252373.1| (thiamine-phosphate diphosphorylase [Zhongshania aliphaticivorans] >gi|385277993|gb|EIF41966.1| thiamine-phosphate pyrophosphorylase [gamma proteobacterium BDW918] >gi|1004370781|gb|AMO69845.1| hypothetical protein AZF00_16735 [Zhongshania aliphaticivorans])

HSP 1 Score: 387.497 bits (994), Expect = 1.556e-125
Identity = 230/501 (45.91%), Postives = 315/501 (62.87%), Query Frame = 0

Query:   71 PIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAAVAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVTSRVEEGSKKRPGPFAECERDMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLV--DVPLVAIGGIGLEAAQGVRDAGADGICVISALTQASD 569
            PIVWSIAGSDSGGGAG+QADL  FH  G HGC+VITA+TAQNS  V  V V+    L   +  L+ D+ A  IK+GMLA   VV  V  +L     +   G V+CDPV+ +S+G  L D      +I+ + PR  L+TPN  EAE ++   ++  +D+ERAA D++A+G   AVL+KGGH L     +R              DY   ++G  G W++   ++  NTHGTGCTLSSA+AA LA+   L DA+V+AKAYVSQG+R   +LG GPGPV   GWP    DFP ++ R+ + S  RP  F  CE ++GLY  V SA W++RLL  GVK IQLR+K      L  EVA A    R+ +AR+++ND+W+LA+   AYG+H+GQED+D         + A+ + G+RLGVSTHT+ E+ RA  L+PSYI++GP++ TTSK + + PQGL +LR+W  ++  +  L AIGGI ++ A+ V   G   I +ISA+TQA +
Sbjct:    7 PIVWSIAGSDSGGGAGIQADLATFHDFGVHGCTVITAITAQNSFAVGHVSVTPRRTLAAQINALDSDLQADVIKLGMLADEGVVGMVAKYL-----DSYRGFVVCDPVLSSSTGGDLTDGNLSACLIEKLLPRCDLLTPNLSEAESILNRRIKGLDDVERAAADIVALGAR-AVLIKGGH-LTARNNQR-------------HDYW--TDGIDGFWLSGANVDTLNTHGTGCTLSSAIAASLARGYALPDALVIAKAYVSQGLRMGVQLGGGPGPVAHVGWPQQLIDFPSLSRRLPQ-SGPRP-VFPSCEGELGLYPVVDSADWIKRLLACGVKTIQLRVKGLQGEALRKEVARAVALGREYSARVFINDFWQLAIAEGAYGVHLGQEDLDI------ADIEAIASAGLRLGVSTHTYYEVARAHGLQPSYIAIGPVYRTTSKDMPYSPQGLMQLRRWVGVLGDNYTLTAIGGIDIKRAKEVLLTGVKSIAMISAITQAEN 477          

BLAST of EWM27690.1 vs. NCBI_GenBank
Match: gi|219122347|ref|XP_002181508.1| (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >gi|217406784|gb|EEC46722.1| predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 384.03 bits (985), Expect = 4.452e-123
Identity = 241/558 (43.19%), Postives = 333/558 (59.68%), Query Frame = 0

Query:   70 PPIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAAVAQDYLLES-----------NGKSGLWITSPRLENQNTHGTGCTLSSAVAAFL-----------------AQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVTSRVEEGSKKRP--------GPFAECERDMG-LYACVASAVWVQRLLE-LGVKDIQLRIK----DRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLV--DVPLVAIGGIG-LEAAQGVRDAGADGICVISALTQASDVAQAVQAWRAVLR 582
            PPIV++IAGSDSGGGAG+QADL A H+ GCHGCS IT LTAQNS  V  V     D LR  L TL +D+P +AIKIGML T ++   VG FL +         V+ DPVM+T+SG  L +E A++AM+  +FP   ++TPNK EAE L+  TL T  D+E  A DL+A+G + +VL+KGGH L          G+     A AQDY L S           +G  G+W+ SPR E ++THGTGCTLS+++AA L                    +  VDA  LAKAYV+ GI    +LG+GPGPV QTG+P+S   FP V +   E  ++ P          + +    +G +   V   VWVQRL +  GV DIQLR+K    ++  LE+   + +  + C+ +  RLWVNDYW+ A+    +G+HVGQED+   + +  L L  ++ + +  G+STH++ EL  A+ ++PSYISLGP+F+T+SK V+F PQGL+ +R+WR L+  +VPLV IGG    E A+ VR  GA+ + VI A+TQA+D  +AV      +R
Sbjct:   36 PPIVYTIAGSDSGGGAGIQADLHAIHSFGCHGCSAITCLTAQNSVGVIGVHAPPPDFLRAQLETLLEDLPPQAIKIGMLGTKELAIEVGAFLKKLKALDRKVWVVLDPVMITTSGHRLIEEDAQEAMVKHVFPHIDVLTPNKFEAEALLNRTLETISDVEEGAKDLIALG-APSVLIKGGHTL-------YEGGKASNHIAYAQDYFLSSVQRNTGEPRLCDGDLGVWLRSPRYETEHTHGTGCTLSASLAASLALGEQERQKPDGKRRGATSAIDTVDACCLAKAYVTAGIFHGIQLGQGPGPVAQTGFPSSHQYFPMVVADAAEDHQRFPRMKAYDDKTTYDDNRPTLGRILPVVNDEVWVQRLCQDPGVHDIQLRVKGIDDNKKILEI---IKKCQKLCQASGKRLWVNDYWQEAIESGCFGVHVGQEDLYTCIRAGGLQL--LREKRMAFGISTHSYGELATALGVKPSYISLGPVFATSSKTVQFDPQGLSLVRKWRELIQKEVPLVVIGGFSDAERAKAVRGLGANCVAVIGAVTQANDTVEAVSQMNEAMR 580          

BLAST of EWM27690.1 vs. NCBI_GenBank
Match: gi|654397094|ref|WP_027873171.1| (bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase [Spongiibacter marinus])

HSP 1 Score: 378.252 bits (970), Expect = 4.764e-122
Identity = 223/501 (44.51%), Postives = 309/501 (61.68%), Query Frame = 0

Query:   71 PIVWSIAGSDSGGGAGVQADLQAFHALGCHGCSVITALTAQNSHQVRKVEVSSLDMLRTTLATLEDDMPAKAIKIGMLATADVVRAVGIFLSEKATEGSSGAVICDPVMVTSSGSVLQDELARQAMIDTIFPRATLITPNKHEAEMLVGYTLRTPEDLERAAHDLLAMGKSGAVLVKGGHALDGLGGERSASGEGLFDAAVAQDYLLESNGKSGLWITSPRLENQNTHGTGCTLSSAVAAFLAQDLPLVDAVVLAKAYVSQGIRAARKLGRGPGPVQQTGWPASASDFPWVTSRVEEGSKKRPGPFAECERDMGLYACVASAVWVQRLLELGVKDIQLRIKDRPALELEHEVAEAAESCRKANARLWVNDYWELAVRYKAYGLHVGQEDMDRLMNSPDLPLVAVKNEGIRLGVSTHTFAELGRAIALRPSYISLGPIFSTTSKKVRFGPQGLARLRQWRHLVD--VPLVAIGGIGLEAAQGVRDAGADGICVISALTQASD 569
            PI W+IAGSDSGGGAG+QADL +FH  G HGCSV+TALTAQNS  V  V ++    L   +  L+ D+ A AIK+GML   DVV  V  +L++       G V+CDPVMV++SG  L ++   +A+I+ I PRA L+TPN  EAE L+   + +P D+ERAA D++ +G   +VL+ GGH                 D     DY   ++G+SG W++   ++  NTHGTGCTLSSA+AA +A+   L DA+V+AKAYVSQG+R + +LG GPGPV   GWP    DFP ++ ++     +    F +C  ++GLY  V S+ W++RLL  GVK IQLRIK      L+ E+  A    ++  AR+++NDYW  A+   AYG+H+GQED+D           A+   G+RLGVSTH++ E+ RA AL PSYI++GP++ TTSK +RFG QGL +LR+W  ++     L AIGGI    A  V   G     +I+A+T+A D
Sbjct:    7 PIAWTIAGSDSGGGAGIQADLVSFHDFGVHGCSVVTALTAQNSFTVGHVAITPRRALAAQINALDSDLQAAAIKLGMLGGEDVVEMVAKYLADY-----RGFVVCDPVMVSTSGGQLMEDGVSEALIEKILPRADLLTPNISEAETLLNREINSPADVERAAQDIVVLGAR-SVLLTGGHLST--------------DDGCRHDYW--TDGESGFWLSGENIDTLNTHGTGCTLSSAIAALIARGYELADALVIAKAYVSQGLRLSVQLGGGPGPVVHLGWPQRLEDFPRLSRQLPAAPIQ---AFPDCGGELGLYPVVDSSEWLERLLRAGVKTIQLRIKIPEQSNLDEEIRRAVALGQQYGARVFINDYWRQAIDAGAYGVHLGQEDLDIADIG------AIAAAGLRLGVSTHSYYEIARAHALSPSYIAIGPVYPTTSKVMRFGAQGLTQLRRWVDILSPRYTLTAIGGIDRRRASDVLATGVGSCAMITAITEADD 476