EWM28428.1, cds2245 (CDS) Nannochloropsis gaditana | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

Overview


Name	EWM28428.1
Unique Name	cds2245
Type	CDS
Organism	Nannochloropsis gaditana (N. gaditana B-31)
Alignment location	CM002458.1:1269278..1269303 -
Alignment location	CM002458.1:1267001..1269167 -

Link to JBrowse

Properties


Property Name	Value
Protein id	EWM28428.1
Product	methionine synthase mitochondrial precursor-like protein
Orig transcript id	gnl\|cribi\|Naga_100388g6.10439.mrna
Gbkey	CDS

Mutants

Expression

No biomaterial libraries express this feature.

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
CM002458.1	supercontig	CM002458.1:1269278..1269303 -
CM002458.1	supercontig	CM002458.1:1267001..1269167 -

Analyses

This CDS is derived from or has results from the following analyses


Analysis Name	Date Performed
GO annotation for N. gaditana B31	2020-04-08
BLAST analysis for N. gaditana B-31	2020-04-07
InterPro analysis for N. gaditana B-31	2020-04-06
Gene prediction for N. gaditana B-31	2014-02-18

Annotated Terms

The following terms have been associated with this CDS:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0000166	nucleotide binding
GO:0016491	oxidoreductase activity
GO:0005506	iron ion binding
GO:0010181	FMN binding

Vocabulary: **INTERPRO**
Term	Definition
IPR017938	Riboflavin_synthase-like_b-brl
IPR023173	NADPH_Cyt_P450_Rdtase_dom3
IPR008254	Flavodoxin/NO_synth
IPR003097	FAD-binding_1
IPR029039	Flavoprotein-like
IPR001094	Flavdoxin

Homology

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BLAST of EWM28428.1 vs. NCBI_GenBank
Match: gi|585110818|gb|EWM28428.1| (methionine synthase mitochondrial precursor-like protein [Nannochloropsis gaditana])

HSP 1 Score: 1471.06 bits (3807), Expect = 0.000e+0
Identity = 730/730 (100.00%), Postives = 730/730 (100.00%), Query Frame = 0

Query:    1 MVNTDGDASHPSILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKKLSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALMKALGRDEAGAKDTALASVGNGEASNKANDDSTLGVGPETGREGMAGQGEEGAPAMEVERVAVEGKVEEKDAEPHVADRVGLPQARGGPLTQASGAQWVESLLAPADFQQIRETALAAAKDRPWTAYTPSVKVVFMDESGPGAGCTPPVAEAHPIPRRGSPPRSSTSSQSSLPCSTPFSHGGAFPTPSAAPSPTALKPPSPFKGRLSPFTGVTGGGRAAPTGSAPVATPQAQGASYSYEHPAQAKVRHAVYLTKTAGDGHHSEPPTRRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVGREGGRAGNRKSRLQVTDGVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQDEGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPRYLLCGVLPLGLSHPATYRLQRGRVRAPTSRGGGSDGRVSAPPGWKACCERRPGPPVGTTSASIRRPLPPPSLPPSPLYLKTGQGIRPSRQPLLAPDFGGARNGRGSVHGVFVPPTGAERGLETLAVRSVLRLLAGGF 730
            MVNTDGDASHPSILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKKLSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALMKALGRDEAGAKDTALASVGNGEASNKANDDSTLGVGPETGREGMAGQGEEGAPAMEVERVAVEGKVEEKDAEPHVADRVGLPQARGGPLTQASGAQWVESLLAPADFQQIRETALAAAKDRPWTAYTPSVKVVFMDESGPGAGCTPPVAEAHPIPRRGSPPRSSTSSQSSLPCSTPFSHGGAFPTPSAAPSPTALKPPSPFKGRLSPFTGVTGGGRAAPTGSAPVATPQAQGASYSYEHPAQAKVRHAVYLTKTAGDGHHSEPPTRRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVGREGGRAGNRKSRLQVTDGVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQDEGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPRYLLCGVLPLGLSHPATYRLQRGRVRAPTSRGGGSDGRVSAPPGWKACCERRPGPPVGTTSASIRRPLPPPSLPPSPLYLKTGQGIRPSRQPLLAPDFGGARNGRGSVHGVFVPPTGAERGLETLAVRSVLRLLAGGF
Sbjct:    1 MVNTDGDASHPSILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKKLSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALMKALGRDEAGAKDTALASVGNGEASNKANDDSTLGVGPETGREGMAGQGEEGAPAMEVERVAVEGKVEEKDAEPHVADRVGLPQARGGPLTQASGAQWVESLLAPADFQQIRETALAAAKDRPWTAYTPSVKVVFMDESGPGAGCTPPVAEAHPIPRRGSPPRSSTSSQSSLPCSTPFSHGGAFPTPSAAPSPTALKPPSPFKGRLSPFTGVTGGGRAAPTGSAPVATPQAQGASYSYEHPAQAKVRHAVYLTKTAGDGHHSEPPTRRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVGREGGRAGNRKSRLQVTDGVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQDEGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPRYLLCGVLPLGLSHPATYRLQRGRVRAPTSRGGGSDGRVSAPPGWKACCERRPGPPVGTTSASIRRPLPPPSLPPSPLYLKTGQGIRPSRQPLLAPDFGGARNGRGSVHGVFVPPTGAERGLETLAVRSVLRLLAGGF 730

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BLAST of EWM28428.1 vs. NCBI_GenBank
Match: gi|585715577|ref|XP_002741834.2| (PREDICTED: methionine synthase reductase-like [Saccoglossus kowalevskii])

HSP 1 Score: 176.022 bits (445), Expect = 1.797e-42
Identity = 172/612 (28.10%), Postives = 256/612 (41.83%), Query Frame = 0

Query:   13 ILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKKLSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALMKALGRDEAGAKDTALASVG----NGEASNKANDDSTLGVGPETGR--------------EGMAGQGEEGAPAMEVERVAVEGKVEEKDAEPHVADR-VGLPQARGGPLTQASGAQWVESLLAPADFQQIRETALAAAKDRPWTAYTPSVKVVFMDESGPGAGCTPPVAEAHPIPRRGSPPRSSTSSQSSLPCST-PFSHGGAFPTPSAAPSPTALKPPSPFKGRLSPFTGVTGGGRAAPTGSAPVATPQAQGASY--SYEHPAQAKVRHAVYLTKTAGDGHHSEPPTRRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVG--REGGR-------AGNRKSRLQVTDGVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQDEGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPR 593
             L+ +GS TG +E IA+ I  + +N HGL  A L  ++  +K  N   E+    +FV +TTG+GD P+NA KF+R LK++T P   L  ++ + +LGLGD+NY  FC+ GK+ D RL+++GG+R +  G AD+AVGLE  VEPWIE L+ AL + L  D +   D    ++     N +       DS + V  ET R              + ++         +  + V   G       E  V D+ V   Q       Q +     ++ +   + Q+  E   +A  D+              D    G       AE  PIP              SL  ST P S  G               PPSP      P+ G+     AA   S     P   GA++         A +  A  LT        S    +  L ++LD         Y PGD++GI CPN  E V+ ++ R+    E  +          +K R  + + +  G  +  A  T        +D+  TPK  +L+ L  YT +  ++  L  L +    G   YE WI    LS+ ++L   PS  P +  L +LLP L PR
Sbjct:   24 FLLLYGSQTGQAEAIAEEIQEDAEN-HGLK-ADLFCLSQTEKKFNIVDEK--CVVFVVSTTGDGDPPDNATKFYRRLKKKTLPNDHLK-QLKYTLLGLGDSNYSNFCNNGKNFDKRLNDLGGQRFYPSGFADDAVGLEIVVEPWIEGLWKALRQELKMDHSNHDDVQTENLSKELENIKLVENEQTDSLIEVS-ETERTKRNETEIDAIIPNKEISNSNTVNQNLLNDQSVNQSGISSNTTNEETVNDQSVDQSQTSSHKTNQETLNNQSKASIEIQNLQEANEKYESAVDDKK------------TDTVNRGDDSDVSQAET-PIP--------------SLKVSTPPLSESG------------LTLPPSP-----HPYIGIQYISDAALDVS---TIPLQGGANFPSCNSEVVMATIMSAERLT--------SSDALKTCLRIKLDIT--GTILSYQPGDSIGIVCPNMEEEVDFLIRRLSILDEADKPITLHVLKDTKKKRALLPEYIPAGCSIRHAILT-------CLDIRNTPKKALLRMLVDYTTNTDEKRRLQELCS--RQGAQDYEKWIRCCSLSITDILVAFPSCNPPIERLFELLPRLQPR 563

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BLAST of EWM28428.1 vs. NCBI_GenBank
Match: gi|1210523396|dbj|GAX17720.1| (methionine synthase reductase [Fistulifera solaris])

HSP 1 Score: 166.392 bits (420), Expect = 2.357e-39
Identity = 168/614 (27.36%), Postives = 258/614 (42.02%), Query Frame = 0

Query:    8 ASHPSILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKKLSNPTFEE----YEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALMKALGRDEAGAKDTALASVGNGEASNKANDDSTLGVGPETGREGMAGQGEEGAPAMEVERVAVEGKVEEKDAEPHVADRVGLPQARGGPLTQASGAQWVESLLAPADFQQIRETALAAAKDRPWTAYTPSVKVVFMDESGPGAGCTPPVAEAHPIPRRGSPPRSSTSSQSSLPCSTPFSHGGAFPTPSAAPSPTALKP--PSPFKGRLSPFTGVTGGGRAAPTGSAPVATPQAQGASYSYEHPAQA-KVRHAVYLTKTAGDGHHSEPPTR---RVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVGREGGRAGNRKSRLQVTDGVAVGGVLGTACWTLEEVVREVMDLEGTPK-PHVLKALAAYTQDEGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAA-PTLAALLDLLPPLPPRYLLCGVLPLGLSHPAT 609
            +   ++ + +GSATGN+E IAK + A  +            ++ +K+   PT+E+      A + V +TTGNGDAPENA +F R++KR           + +AVLGLGDTNYD+FC   K +D +L E+GG R+  L  ADEA GLE  VEPW E +          DE  A     A  G+  ++ +  ++S   +           Q +E    + VE  A E K    D    V   +GL + R  P+       W+                       P T Y PS+              T   +    +PR+ S               T FS   +  T S+    T   P   +P K R      +T     A   +  +     +   +S E   +A KV  + +      D    E   R   RV+EL L   D D    Y PGD++G+   N  E V  V++ + +  G   N++  + + +G AV         T+EEV R   DL    K   +L  L+ +  ++ ++  L +L +    G  +++S++ EQRL++ ++L   PS    ++  L  +LP LPPRY      PL  S   T
Sbjct:    2 SEESTLFILYGSATGNAEHIAKDLAA--RPNTYFDKVLCFELDQFKRKCQPTWEKETTTKHAVLIVTSTTGNGDAPENASRFVRYIKRSQTVDEKPFRHVAYAVLGLGDTNYDQFCQCAKVIDKKLLELGGTRIRPLTCADEATGLEEAVEPWTERIL---------DEIAA-----ACRGSNCSTEEVKEESKEAI-----------QEKEVDQTLTVENGARELKF--SDGVATVRALIGL-ENRSQPI-------WI-----------------------PDTKYLPSL--------------TASRSCELLVPRKES---------------TDFSCSDSLTTTSSGLHYTMKHPFESTPLKARY-----LTNTSTKAAEEATGLLEFTEEAEMFSDESLLKASKVFESHFSLSPEDDSCTREQRDRNGKRVIELSLALPD-DYTLEYAPGDSLGLLVENTPETVLFVIDMLQKNHGIHSNQQ--VSIDEGRAV---------TVEEVARSHFDLCSPVKSKRILFGLSQFATNDEERVALQFLSSKTTEGDRIFKSFVDEQRLNVVDLLREFPSTQGISMQGLFGMLPALPPRYYSVCSSPLKNSTSVT 509

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BLAST of EWM28428.1 vs. NCBI_GenBank
Match: gi|780138304|ref|XP_011680278.1| (PREDICTED: methionine synthase reductase isoform X1 [Strongylocentrotus purpuratus] >gi|780138309|ref|XP_011680279.1| PREDICTED: methionine synthase reductase isoform X2 [Strongylocentrotus purpuratus])

HSP 1 Score: 158.688 bits (400), Expect = 7.797e-37
Identity = 174/591 (29.44%), Postives = 256/591 (43.32%), Query Frame = 0

Query:   13 ILVCFGSATGNSEFIAKRIHAELQNKHGLPG-ARLLAMNDWKKLSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALMKALGR----DEAGAKDTALASVGNGEASNKANDDSTLGVGPETGREGMAGQGEEGAPAMEVERVAVEGKVEEKDAEPHVADRVGLPQARGGPLTQASG--AQWVESLLAPADFQQIRETALAAAKDRPWTAYTPSVKVVFMDESGPGAGCTPPVAEAHPIPRRGSPPRSSTSSQSSLPCSTPFSHGGAFPTPSAAPSPTALKPPSPFKGRLSPFTGVTGGGRAAPTGSAPVATPQAQGASYSYEHPAQAKVRHAVYLTKTAGDGHHSEPPTRRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVG-REGGRAGNRKSRLQVTD--GVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQDEGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPR 593
             L+ +GS TG S+ IA+ IH EL +KH L      L++ + KK S     +    + V +TTG G+ PE A KFWR L++ T P   LAN ++FA+L LGD+NY  FC  GK+ D RL E+G + ++  G AD+A GLE  VEPWIE L+ AL + + +    D  G  +     + NGE + +  ++  +G   E      +G  + G+  +          V+   A+P    + G   + G   +  SG      +S   P +   I +T          T+  P +KV   +ES  G    P V+                S   SLP   P S  G   T  A P P  LK        +   +    GG A P+ S+ V                QA V  A  LT+        +   +  L++EL   +    F   PGD+  I CPN    V  +L R+G RE      R S L+ T     AV   + T   TL  +     D+   PK   L+ L  YT D  ++  L  L +  + G+  Y +++    LSL +VL   PS  P +  +L+ +  L PR
Sbjct:    6 FLLLYGSETGQSQAIAEEIH-ELSSKHDLESEIHCLSLTE-KKFS---LSKETCMVMVVSTTGEGECPETAMKFWRRLRKTTLPSDHLAN-LNFALLALGDSNYSNFCRNGKNFDERLQELGAKHIYPTGYADDATGLEVVVEPWIEGLWKALHEVMDKQGTPDSNGCLENGHLEI-NGERNKENRNERMIGENAE------SGTTQNGSLDI----------VQADQAQP----KTGEQSSTGNNTSAMSGNDVNGSDSPDHPGNSNNITQTLQRTPSQS--TSTDPFIKV---EESTDGKAEGPTVS----------------SLTHSLP---PLSESGL--TLPALPPP-FLKVVYAHNETIDLESLPIQGGAALPSASSGV---------------IQAMVVQATQLTR--------DDAVKTALDIELKVAENSMSFQ--PGDSFSIICPNDAGEVTDLLHRLGLREMADVPLRISVLEGTKKRKAAVADFVPTFS-TLRYIFTTCCDVRVLPKKAFLRTLVEYTSDPKEKRRLQELCS--KQGQPDYAAFLRAPSLSLMDVLIAFPSCLPPIERILEHMTRLKPR 514

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BLAST of EWM28428.1 vs. NCBI_GenBank
Match: gi|390347070|ref|XP_787027.3| (PREDICTED: methionine synthase reductase isoform X3 [Strongylocentrotus purpuratus])

HSP 1 Score: 158.688 bits (400), Expect = 8.931e-37
Identity = 174/591 (29.44%), Postives = 256/591 (43.32%), Query Frame = 0

Query:   13 ILVCFGSATGNSEFIAKRIHAELQNKHGLPG-ARLLAMNDWKKLSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALMKALGR----DEAGAKDTALASVGNGEASNKANDDSTLGVGPETGREGMAGQGEEGAPAMEVERVAVEGKVEEKDAEPHVADRVGLPQARGGPLTQASG--AQWVESLLAPADFQQIRETALAAAKDRPWTAYTPSVKVVFMDESGPGAGCTPPVAEAHPIPRRGSPPRSSTSSQSSLPCSTPFSHGGAFPTPSAAPSPTALKPPSPFKGRLSPFTGVTGGGRAAPTGSAPVATPQAQGASYSYEHPAQAKVRHAVYLTKTAGDGHHSEPPTRRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVG-REGGRAGNRKSRLQVTD--GVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQDEGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPR 593
             L+ +GS TG S+ IA+ IH EL +KH L      L++ + KK S     +    + V +TTG G+ PE A KFWR L++ T P   LAN ++FA+L LGD+NY  FC  GK+ D RL E+G + ++  G AD+A GLE  VEPWIE L+ AL + + +    D  G  +     + NGE + +  ++  +G   E      +G  + G+  +          V+   A+P    + G   + G   +  SG      +S   P +   I +T          T+  P +KV   +ES  G    P V+                S   SLP   P S  G   T  A P P  LK        +   +    GG A P+ S+ V                QA V  A  LT+        +   +  L++EL   +    F   PGD+  I CPN    V  +L R+G RE      R S L+ T     AV   + T   TL  +     D+   PK   L+ L  YT D  ++  L  L +  + G+  Y +++    LSL +VL   PS  P +  +L+ +  L PR
Sbjct:    6 FLLLYGSETGQSQAIAEEIH-ELSSKHDLESEIHCLSLTE-KKFS---LSKETCMVMVVSTTGEGECPETAMKFWRRLRKTTLPSDHLAN-LNFALLALGDSNYSNFCRNGKNFDERLQELGAKHIYPTGYADDATGLEVVVEPWIEGLWKALHEVMDKQGTPDSNGCLENGHLEI-NGERNKENRNERMIGENAE------SGTTQNGSLDI----------VQADQAQP----KTGEQSSTGNNTSAMSGNDVNGSDSPDHPGNSNNITQTLQRTPSQS--TSTDPFIKV---EESTDGKAEGPTVS----------------SLTHSLP---PLSESGL--TLPALPPP-FLKVVYAHNETIDLESLPIQGGAALPSASSGV---------------IQAMVVQATQLTR--------DDAVKTALDIELKVAENSMSFQ--PGDSFSIICPNDAGEVTDLLHRLGLREMADVPLRISVLEGTKKRKAAVADFVPTFS-TLRYIFTTCCDVRVLPKKAFLRTLVEYTSDPKEKRRLQELCS--KQGQPDYAAFLRAPSLSLMDVLIAFPSCLPPIERILEHMTRLKPR 514

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BLAST of EWM28428.1 vs. NCBI_GenBank
Match: gi|1173945117|gb|OQR93784.1| (NADPH-cytochrome P450 reductase [Achlya hypogyna])

HSP 1 Score: 155.992 bits (393), Expect = 2.885e-36
Identity = 81/167 (48.50%), Postives = 109/167 (65.27%), Query Frame = 0

Query:    7 DASHPSILVCFGSATGNSEFIAKRIHAE-LQNKHGLPGARLLAMNDWKKLSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALMKALGRDE 172
            DAS P + V +GS TG +E IA+R+ ++ ++     P  R L   D  + S          + VC+TTGNGD P NAEKFWRF+K+R  P TLLAN + F VLGLGDTNYDKFCHMGKS+  R+ ++G    ++LG ADEA+GLE +VEPWI+  +PAL+  +   +
Sbjct:    3 DASKP-LYVFYGSQTGCAESIAERLQSDAIERNINCPEVRPL---DAFEKSGVLQAANATVVIVCSTTGNGDPPGNAEKFWRFIKKRAHPATLLAN-LEFTVLGLGDTNYDKFCHMGKSIFRRMKDLGAVSFYDLGCADEAMGLEDSVEPWIDGFWPALVGVISSSQ 164

HSP 2 Score: 82.8037 bits (203), Expect = 9.727e-13
Identity = 68/221 (30.77%), Postives = 98/221 (44.34%), Query Frame = 0

Query:  383 APTGSAPVATPQ-AQGASYSYEHPAQAKVRHAVYLTKTAGDGHHSEPPTRRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVGREGGRAGNRKSRLQVTDGVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQDEGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPRYLLCGVLPL 602
            APT +      Q A    YS   P  A +  A YLT  A          R+VL L+LD         YVPGD VGI CPN    V+ +++R+     +    +     +    V G+      TL ++    +DL   PK   L+ LA +  D  ++A L+      + G A ++ ++ +Q LS+ EVL L  S  P L  +L L+P L PRY      PL
Sbjct:  234 APTDAIAATVAQVATDTLYSAASPFPATIAAARYLTTPAS-------ADRQVLALDLDISGSGAT--YVPGDVVGINCPNDAAVVDYLIQRLQLSAFQHHRVQIAPVASAKKKVAGMPFPPSATLRDIFSTQLDLAALPKKATLRGLAVHCLDADERARLF------KEGAADFKRFVDDQYLSIPEVLALFQSCHPPLDVMLSLVPRLSPRYYSVASSPL 439

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BLAST of EWM28428.1 vs. NCBI_GenBank
Match: gi|673041971|ref|XP_008874946.1| (hypothetical protein, variant 1 [Aphanomyces invadans] >gi|574469669|gb|ETV96683.1| hypothetical protein, variant 1 [Aphanomyces invadans])

HSP 1 Score: 156.762 bits (395), Expect = 4.209e-36
Identity = 83/164 (50.61%), Postives = 112/164 (68.29%), Query Frame = 0

Query:    7 DASHPSILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKK-----LSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALM 165
            D++   + + FGS TG +E IAKRI A+    H +P + L  +N ++K     L+ PT       + VC+TTGNGD P NAEKFWRFLK++  P+TLL   + F+VL LGDTNYDKFC+MGKS+  R+ E+GG  MH+LG ADEA+GLE TVEP++  L+ AL+
Sbjct:  202 DSAVDHLHIFFGSQTGCAESIAKRIQADAAGHH-IPTS-LNPLNAFEKEGILALATPTHV-----VIVCSTTGNGDPPTNAEKFWRFLKKKAHPRTLL-QHVRFSVLALGDTNYDKFCYMGKSIHRRMKELGGVCMHDLGCADEAMGLEDTVEPYVLGLWSALL 357

HSP 2 Score: 144.05 bits (362), Expect = 5.529e-32
Identity = 73/155 (47.10%), Postives = 102/155 (65.81%), Query Frame = 0

Query:   13 ILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKK---LSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPAL 164
            + V +GS TG +E IAKR+ ++   +H      L  +N+++K   L++P+       + VC+TTGNGD P NAEKFWR+LK++  P TLL   + F VL LGDTNYDKFC+MGK++  RL E+G +  + LG ADEA+GLE  VEPW+   + AL
Sbjct:    6 LFVFYGSQTGCAESIAKRVQSDGLERHM--TVALHTLNEFEKCGVLTHPS----ATVLIVCSTTGNGDPPTNAEKFWRYLKKKAHPTTLL-EHVRFTVLALGDTNYDKFCYMGKNIHRRLGELGAKSYYMLGCADEAMGLEDCVEPWLMGHWAAL 153

HSP 3 Score: 85.8853 bits (211), Expect = 1.191e-13
Identity = 58/174 (33.33%), Postives = 84/174 (48.28%), Query Frame = 0

Query:  431 RRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVGREGGRAGNRKSRLQVTDGVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQD-EGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPRYLLCGVLPLG 603
            R+VL LELDT        YVPGD++GIRCPN    V   + R+G     + +      V   V    +   A  +L  ++    DL+   K   L+ LA Y  D   D+  L +L +  + G   ++ ++  QRLSL ++L L P+  P +A +L LLP L PRY      PL 
Sbjct:  476 RKVLWLELDTAGMG--MTYVPGDSIGIRCPNHANVVSYFVRRLGI----SSDELHAPCVIQSVKKAAISANAV-SLFHLLTHHYDLQAQVKKAALRVLAQYCPDGSDDRRTLLYLCS--KDGPTHFQQFVEAQRLSLVDILHLFPTCTPPIAHILSLLPTLSPRYYSIASSPLS 640

[Close]

BLAST of EWM28428.1 vs. NCBI_GenBank
Match: gi|570971657|gb|ETP34776.1| (hypothetical protein F442_16929 [Phytophthora parasitica P10297])

HSP 1 Score: 155.221 bits (391), Expect = 7.507e-36
Identity = 82/182 (45.05%), Postives = 117/182 (64.29%), Query Frame = 0

Query:   12 SILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKKLSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALMKALGRDEAGAKDTALASVGNGEASNKAN 193
            ++ V +GS TG +E IA+RI+ E   K     A L  +N+++K    T + +   + VC+TTGNGD P+N+ KFWR++KRRTQP+ +LA ++ + VLGLGDTNYDKFC MGKS++ R+ E+G +  +  G ADEA+GLE +VEPW+  L+ A  KA G  EA A      S      +N+ N
Sbjct:   26 AVSVFYGSQTGCAESIAQRIYDEALEKQ--LDAELHPLNEFQKSKVETRDGHFV-VIVCSTTGNGDPPDNSGKFWRYVKRRTQPEDMLA-KLRYTVLGLGDTNYDKFCFMGKSIEKRMRELGAQSFYEFGAADEAMGLEDSVEPWLNGLWEAFDKANGVIEATADVVTSVSDIKESTANRGN 203

HSP 2 Score: 100.523 bits (249), Expect = 3.286e-18
Identity = 75/225 (33.33%), Postives = 106/225 (47.11%), Query Frame = 0

Query:  391 ATPQAQGASYSYEHPAQAKVRHAVYLTKTAGDGHHSEPPTRRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVGREG-------------GRAGNRKSRLQVTDGVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQDEGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPRYLLCGVLPL 602
            +T        S  +P  A +  A YLT       H+E   R+VL L++D         YVPGD++GI+CPNRTE V+ +L R+G  G             GRA  RK+               T C ++ +V    +D+  +PK   ++ALA Y  DE ++A +  L +  + G   Y  +I EQ L+ AE+L   PS  P L  LL L+P L PRY      PL
Sbjct:  274 STTDEDDKEISSTNPFMASLIGAEYLT-----APHAE---RKVLRLDIDVSG--SGIQYVPGDSIGIKCPNRTEDVDALLARLGVSGDEVFSAEPVAATNGRAAKRKN---------TSIHFPTPC-SVRDVFLHYVDILSSPKKAAVRALATYCTDEEERARMLLLSS--KTGADKYTEFITEQHLNFAEILHFFPSCKPPLDHLLTLIPQLMPRYYSIATSPL 476

[Close]

BLAST of EWM28428.1 vs. NCBI_GenBank
Match: gi|673041969|ref|XP_008874945.1| (hypothetical protein H310_10357 [Aphanomyces invadans] >gi|574469668|gb|ETV96682.1| hypothetical protein H310_10357 [Aphanomyces invadans])

HSP 1 Score: 155.606 bits (392), Expect = 1.235e-35
Identity = 83/164 (50.61%), Postives = 112/164 (68.29%), Query Frame = 0

Query:    7 DASHPSILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKK-----LSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALM 165
            D++   + + FGS TG +E IAKRI A+    H +P + L  +N ++K     L+ PT       + VC+TTGNGD P NAEKFWRFLK++  P+TLL   + F+VL LGDTNYDKFC+MGKS+  R+ E+GG  MH+LG ADEA+GLE TVEP++  L+ AL+
Sbjct:  202 DSAVDHLHIFFGSQTGCAESIAKRIQADAAGHH-IPTS-LNPLNAFEKEGILALATPTHV-----VIVCSTTGNGDPPTNAEKFWRFLKKKAHPRTLL-QHVRFSVLALGDTNYDKFCYMGKSIHRRMKELGGVCMHDLGCADEAMGLEDTVEPYVLGLWSALL 357

HSP 2 Score: 143.28 bits (360), Expect = 1.362e-31
Identity = 73/155 (47.10%), Postives = 102/155 (65.81%), Query Frame = 0

Query:   13 ILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKK---LSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPAL 164
            + V +GS TG +E IAKR+ ++   +H      L  +N+++K   L++P+       + VC+TTGNGD P NAEKFWR+LK++  P TLL   + F VL LGDTNYDKFC+MGK++  RL E+G +  + LG ADEA+GLE  VEPW+   + AL
Sbjct:    6 LFVFYGSQTGCAESIAKRVQSDGLERHM--TVALHTLNEFEKCGVLTHPS----ATVLIVCSTTGNGDPPTNAEKFWRYLKKKAHPTTLL-EHVRFTVLALGDTNYDKFCYMGKNIHRRLGELGAKSYYMLGCADEAMGLEDCVEPWLMGHWAAL 153

HSP 3 Score: 85.1149 bits (209), Expect = 2.401e-13
Identity = 58/174 (33.33%), Postives = 84/174 (48.28%), Query Frame = 0

Query:  431 RRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVGREGGRAGNRKSRLQVTDGVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQD-EGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPRYLLCGVLPLG 603
            R+VL LELDT        YVPGD++GIRCPN    V   + R+G     + +      V   V    +   A  +L  ++    DL+   K   L+ LA Y  D   D+  L +L +  + G   ++ ++  QRLSL ++L L P+  P +A +L LLP L PRY      PL 
Sbjct:  476 RKVLWLELDTAGMG--MTYVPGDSIGIRCPNHANVVSYFVRRLGI----SSDELHAPCVIQSVKKAAISANAV-SLFHLLTHHYDLQAQVKKAALRVLAQYCPDGSDDRRTLLYLCS--KDGPTHFQQFVEAQRLSLVDILHLFPTCTPPIAHILSLLPTLSPRYYSIASSPLS 640

[Close]

BLAST of EWM28428.1 vs. NCBI_GenBank
Match: gi|568009571|gb|ETL83773.1| (hypothetical protein L917_16326 [Phytophthora parasitica])

HSP 1 Score: 154.066 bits (388), Expect = 1.986e-35
Identity = 81/182 (44.51%), Postives = 115/182 (63.19%), Query Frame = 0

Query:   12 SILVCFGSATGNSEFIAKRIHAELQNKHGLPGARLLAMNDWKKLSNPTFEEYEAGIFVCATTGNGDAPENAEKFWRFLKRRTQPKTLLANRMHFAVLGLGDTNYDKFCHMGKSMDTRLSEVGGRRMHNLGTADEAVGLEGTVEPWIETLYPALMKALGRDEAGAKDTALASVGNGEASNKAN 193
            ++ V +GS TG +E IA+RI+ E   K     A L  +N+++K    T + +   + VC+TTGNGD P+N  KFWR++KRRTQP+ +LA ++ + VLGLGDTNYDKFC MGKS++ R+ E+G +  +  G ADEA+GLE +VEPW+  L+ A  KA G  E  A      S      +N+ N
Sbjct:   26 AVSVFYGSQTGCAESIAQRIYDEALEKQ--LDAELHPLNEFQKSKVETCDGHFV-VIVCSTTGNGDPPDNCGKFWRYVKRRTQPEDMLA-KLRYTVLGLGDTNYDKFCFMGKSIEKRMRELGAQSFYEFGAADEAMGLEDSVEPWLNGLWEAFDKANGVIEVTADVVTSVSDIKESTANRGN 203

HSP 2 Score: 100.138 bits (248), Expect = 4.016e-18
Identity = 75/225 (33.33%), Postives = 106/225 (47.11%), Query Frame = 0

Query:  391 ATPQAQGASYSYEHPAQAKVRHAVYLTKTAGDGHHSEPPTRRVLELELDTQDCDPPFPYVPGDAVGIRCPNRTEAVERVLERVGREG-------------GRAGNRKSRLQVTDGVAVGGVLGTACWTLEEVVREVMDLEGTPKPHVLKALAAYTQDEGDQALLWWLGAGGEGGKALYESWILEQRLSLAEVLELIPSAAPTLAALLDLLPPLPPRYLLCGVLPL 602
            +T        S  +P  A +  A YLT       H+E   R+VL L++D         YVPGD++GI+CPNRTE V+ +L R+G  G             GRA  RK+               T C ++ +V    +D+  +PK   ++ALA Y  DE ++A +  L +  + G   Y  +I EQ L+ AE+L   PS  P L  LL L+P L PRY      PL
Sbjct:  274 STTDEDDKEISSTNPFMASLIGAEYLT-----APHAE---RKVLRLDIDVSG--SGIQYVPGDSIGIKCPNRTEDVDALLARLGVSGDEVFSAEPVAATNGRAAKRKN---------TSIHFPTPC-SVRDVFLHYVDILSSPKKAAVRALATYCTDEEERARMLLLSS--KTGADKYTEFITEQHLNFAEILHFFPSCKPPLDHLLTLIPQLMPRYYSIATSPL 476

The following BLAST results are available for this feature:

BLAST of EWM28428.1 vs. NCBI_GenBank

BLAST of EWM28428.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10


Match Name	E-value	Identity	Description
gi\|585110818\|gb\|EWM28428.1\|	0.000e+0	100.00	methionine synthase mitochondrial precursor-like p...	[more]
gi\|585715577\|ref\|XP_002741834.2\|	1.797e-42	28.10	PREDICTED: methionine synthase reductase-like [Sac...	[more]
gi\|1210523396\|dbj\|GAX17720.1\|	2.357e-39	27.36	methionine synthase reductase [Fistulifera solaris...	[more]
gi\|780138304\|ref\|XP_011680278.1\|	7.797e-37	29.44	PREDICTED: methionine synthase reductase isoform X...	[more]
gi\|390347070\|ref\|XP_787027.3\|	8.931e-37	29.44	PREDICTED: methionine synthase reductase isoform X...	[more]
gi\|1173945117\|gb\|OQR93784.1\|	2.885e-36	48.50	NADPH-cytochrome P450 reductase [Achlya hypogyna]	[more]
gi\|673041971\|ref\|XP_008874946.1\|	4.209e-36	50.61	hypothetical protein, variant 1 [Aphanomyces invad...	[more]
gi\|570971657\|gb\|ETP34776.1\|	7.507e-36	45.05	hypothetical protein F442_16929 [Phytophthora para...	[more]
gi\|673041969\|ref\|XP_008874945.1\|	1.235e-35	50.61	hypothetical protein H310_10357 [Aphanomyces invad...	[more]
gi\|568009571\|gb\|ETL83773.1\|	1.986e-35	44.51	hypothetical protein L917_16326 [Phytophthora para...	[more]

Relationships

This CDS is a part of the following mRNA feature(s):


Feature Name	Unique Name	Species	Type
rna2253	rna2253	Nannochloropsis gaditana (N. gaditana B-31)	mRNA

Sequences

Synonyms

Publications