EWM28449.1, cds2225 (CDS) Nannochloropsis gaditana

Overview
NameEWM28449.1
Unique Namecds2225
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002458.1:1222639..1222832 +
Alignment locationCM002458.1:1222999..1223105 +
Alignment locationCM002458.1:1223327..1223940 +
Alignment locationCM002458.1:1224221..1224310 +

Link to JBrowse

Properties
Property NameValue
Protein idEWM28449.1
Productaspartyl asparaginyl beta-hydroxylase
Orig transcript idgnl|cribi|Naga_100627g2.10710.mrna
GbkeyCDS
Noteencoded by transcript variant 1; alternatively spliced
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002458.1supercontigCM002458.1:1222639..1222832 +
CM002458.1supercontigCM002458.1:1222999..1223105 +
CM002458.1supercontigCM002458.1:1223327..1223940 +
CM002458.1supercontigCM002458.1:1224221..1224310 +
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Biological Process
TermDefinition
GO:0006464cellular protein modification process
GO:0018193peptidyl-amino acid modification
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR007803Asp_Arg_Pro-Hydrxlase
Homology
BLAST of EWM28449.1 vs. NCBI_GenBank
Match: gi|585110851|gb|EWM28449.1| (aspartyl asparaginyl beta-hydroxylase [Nannochloropsis gaditana])

HSP 1 Score: 692.189 bits (1785), Expect = 0.000e+0
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0
Query:    1 MSMSTSGPSAIASGFANKLQATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQIDSDYKRQDGEHSLHHGRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEGRREGRREGEKGGSEEGRERGNQGEGLQSAAGKDSSQSRPDPVPPGCASSSAEFDSLATLQARLPCGIRIGDQVREWVEGKCLVFDDAYEHEVWNLTEEERVLLLVDFWHPEIALEEREAIQKMFEFAARQGWMQGNGG 334
            MSMSTSGPSAIASGFANKLQATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQIDSDYKRQDGEHSLHHGRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEGRREGRREGEKGGSEEGRERGNQGEGLQSAAGKDSSQSRPDPVPPGCASSSAEFDSLATLQARLPCGIRIGDQVREWVEGKCLVFDDAYEHEVWNLTEEERVLLLVDFWHPEIALEEREAIQKMFEFAARQGWMQGNGG
Sbjct:    1 MSMSTSGPSAIASGFANKLQATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQIDSDYKRQDGEHSLHHGRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEGRREGRREGEKGGSEEGRERGNQGEGLQSAAGKDSSQSRPDPVPPGCASSSAEFDSLATLQARLPCGIRIGDQVREWVEGKCLVFDDAYEHEVWNLTEEERVLLLVDFWHPEIALEEREAIQKMFEFAARQGWMQGNGG 334          
BLAST of EWM28449.1 vs. NCBI_GenBank
Match: gi|585110852|gb|EWM28450.1| (aspartyl asparaginyl beta-hydroxylase [Nannochloropsis gaditana])

HSP 1 Score: 485.723 bits (1249), Expect = 2.707e-171
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0
Query:  101 GRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEGRREGRREGEKGGSEEGRERGNQGEGLQSAAGKDSSQSRPDPVPPGCASSSAEFDSLATLQARLPCGIRIGDQVREWVEGKCLVFDDAYEHEVWNLTEEERVLLLVDFWHPEIALEEREAIQKMFEFAARQGWMQGNGG 334
            GRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEGRREGRREGEKGGSEEGRERGNQGEGLQSAAGKDSSQSRPDPVPPGCASSSAEFDSLATLQARLPCGIRIGDQVREWVEGKCLVFDDAYEHEVWNLTEEERVLLLVDFWHPEIALEEREAIQKMFEFAARQGWMQGNGG
Sbjct:   47 GRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEGRREGRREGEKGGSEEGRERGNQGEGLQSAAGKDSSQSRPDPVPPGCASSSAEFDSLATLQARLPCGIRIGDQVREWVEGKCLVFDDAYEHEVWNLTEEERVLLLVDFWHPEIALEEREAIQKMFEFAARQGWMQGNGG 280          
BLAST of EWM28449.1 vs. NCBI_GenBank
Match: gi|1173943950|gb|OQR92744.1| (aspartyl/Asparaginyl beta-hydroxylase [Achlya hypogyna])

HSP 1 Score: 158.303 bits (399), Expect = 2.636e-43
Identity = 79/177 (44.63%), Postives = 105/177 (59.32%), Query Frame = 0
Query:   20 QATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQIDSDYKRQDGEHSLHHGRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEG 196
            +A ++RS +  T A   PRPSP+LF FPG+ ++P H      W   L     TI++EY+ L+  Q +SDY     EHSLH G+WDW SY+ KG+   LF + CP T++LL   N     M   PF+++FFSTL  QS I  H+ P N+RLRCH PL VP+G      +E KE  KEG
Sbjct:    3 KAALLRSALCLTTAK--PRPSPTLFMFPGLKAQPFHPTNSFPWTATLAANLDTIREEYVALRHAQKESDYVLAGQEHSLHQGQWDWFSYVTKGKKIDLFEKHCPTTTKLL---NDIPGFMTGLPFAYAFFSTLQPQSAIKAHSAPCNIRLRCHFPLFVPKGCGIRVADETKE-WKEG 173          

HSP 2 Score: 86.6557 bits (213), Expect = 3.062e-16
Identity = 36/71 (50.70%), Postives = 53/71 (74.65%), Query Frame = 0
Query:  261 CGIRIGDQVREWVEGKCLVFDDAYEHEVWNLTEE-ERVLLLVDFWHPEIALEEREAIQKMFEFAARQGWMQ 330
            CGIR+ D+ +EW EG+ +VFDD+Y+HEVW+  ++ ERV+LL D WHP++   E+EA+  MF  A  +GW+Q
Sbjct:  159 CGIRVADETKEWKEGEAMVFDDSYDHEVWHDGQKGERVVLLFDLWHPDLVDGEKEALVNMFAEATDKGWLQ 229          
BLAST of EWM28449.1 vs. NCBI_GenBank
Match: gi|1173958312|gb|OQS04692.1| (aspartyl/Asparaginyl beta-hydroxylase [Thraustotheca clavata])

HSP 1 Score: 161.77 bits (408), Expect = 3.800e-43
Identity = 79/180 (43.89%), Postives = 109/180 (60.56%), Query Frame = 0
Query:   17 NKLQATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQIDSDYKRQDGEHSLHHGRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEG 196
             K +A ++RS +  T A   PRPSP+LF FPG+ S+P H      W ++L E    I++EY+ L+  Q +SDY     EH+LH G+WDW SY++KG+   LF + CP T++LL   N     M   PF+++FFSTL  QS I  H+ P N+RLRCH PL VP+G      ++ KE  KEG
Sbjct:  134 TKAKAALLRSALCLTTAK--PRPSPTLFVFPGLKSQPFHANQDFPWTKLLSENLDIIREEYVALRHAQKESDYVVAGQEHTLHQGQWDWFSYVIKGKKTSLFEQHCPTTTKLL---NDIPGFMTGLPFAYAFFSTLKPQSAIKAHSAPCNIRLRCHFPLFVPKGCGIRVADQTKE-WKEG 307          

HSP 2 Score: 96.2857 bits (238), Expect = 7.648e-19
Identity = 41/71 (57.75%), Postives = 54/71 (76.06%), Query Frame = 0
Query:  261 CGIRIGDQVREWVEGKCLVFDDAYEHEVW-NLTEEERVLLLVDFWHPEIALEEREAIQKMFEFAARQGWMQ 330
            CGIR+ DQ +EW EG+C+VFDDAY+HEVW +  + ERVLLL DFWHP++  EE+ A+  MF  A  +GW+Q
Sbjct:  293 CGIRVADQTKEWKEGECMVFDDAYDHEVWHDGAKGERVLLLFDFWHPDLIEEEKAALIDMFAQATDKGWLQ 363          
BLAST of EWM28449.1 vs. NCBI_GenBank
Match: gi|813157761|ref|XP_012202229.1| (hypothetical protein SPRG_07847 [Saprolegnia parasitica CBS 223.65] >gi|641533137|gb|KDO27139.1| hypothetical protein SPRG_07847 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 156.762 bits (395), Expect = 1.128e-42
Identity = 78/177 (44.07%), Postives = 105/177 (59.32%), Query Frame = 0
Query:   20 QATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQIDSDYKRQDGEHSLHHGRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEG 196
            +A ++RS +  T A   PRPSP+LF FPG+ ++P H+A    W +   +   TI++EY+ L+  Q +SDY     EH+LH G+WDW SY+ KG+   LF   CP T+RLL   N     M   PF+++FFSTL  QS I  H+ P N+RLRCH PL VP        +E KE  KEG
Sbjct:    3 KAALLRSALCLTTAK--PRPSPTLFMFPGLKAQPFHEAKDFPWTQSFTDNLDTIREEYVALRHAQKESDYVLAGQEHALHQGQWDWFSYVTKGKKVDLFETHCPTTTRLL---NAIPGFMTGLPFAYAFFSTLQPQSAIKAHSAPCNIRLRCHFPLFVPPNCGIRVADETKE-WKEG 173          

HSP 2 Score: 88.5817 bits (218), Expect = 6.121e-17
Identity = 36/70 (51.43%), Postives = 53/70 (75.71%), Query Frame = 0
Query:  261 CGIRIGDQVREWVEGKCLVFDDAYEHEVWNLTEE-ERVLLLVDFWHPEIALEEREAIQKMFEFAARQGWM 329
            CGIR+ D+ +EW EG+C+VFDDAY+HEVW+  ++ ERV+LL D WHP++   E++A+  MF  A  +GW+
Sbjct:  159 CGIRVADETKEWKEGECMVFDDAYDHEVWHDGQKGERVVLLFDLWHPDLVPGEKDALINMFADATDKGWL 228          
BLAST of EWM28449.1 vs. NCBI_GenBank
Match: gi|669143896|ref|XP_008607510.1| (hypothetical protein SDRG_03651 [Saprolegnia diclina VS20] >gi|530741207|gb|EQC39449.1| hypothetical protein SDRG_03651 [Saprolegnia diclina VS20])

HSP 1 Score: 155.992 bits (393), Expect = 2.260e-42
Identity = 77/177 (43.50%), Postives = 105/177 (59.32%), Query Frame = 0
Query:   20 QATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQIDSDYKRQDGEHSLHHGRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEG 196
            +A ++RS +  T A   PRPSP+LF FPG+ ++P H+A    W +   +   TI++EY+ L+  Q +SDY     EH+LH G+WDW SY+ KG+   LF + CP T+ LL   N     M   PF+++FFSTL  QS I  H+ P N+RLRCH PL VP        +E KE  KEG
Sbjct:    3 KAALLRSALCLTTAK--PRPSPTLFMFPGLKAQPFHEAKDFAWTQSFTDNLDTIREEYVALRHAQKESDYVLAGQEHALHQGQWDWFSYVTKGKKVDLFEQHCPTTTHLL---NAIPGFMTGLPFAYAFFSTLQPQSAIKAHSAPCNIRLRCHFPLFVPPNCGIRVADETKE-WKEG 173          

HSP 2 Score: 88.5817 bits (218), Expect = 5.590e-17
Identity = 36/70 (51.43%), Postives = 54/70 (77.14%), Query Frame = 0
Query:  261 CGIRIGDQVREWVEGKCLVFDDAYEHEVWNLTEE-ERVLLLVDFWHPEIALEEREAIQKMFEFAARQGWM 329
            CGIR+ D+ +EW EG+C+VFDDAY+HEVW+  ++ ERV+LL D WHP++   E++A+  MF  A+ +GW+
Sbjct:  159 CGIRVADETKEWKEGECMVFDDAYDHEVWHDGQKGERVVLLFDLWHPDLVPGEKDALINMFAEASDKGWL 228          
BLAST of EWM28449.1 vs. NCBI_GenBank
Match: gi|698822973|ref|XP_009844515.1| (hypothetical protein, variant [Aphanomyces astaci] >gi|574100416|gb|ETV65996.1| hypothetical protein, variant [Aphanomyces astaci])

HSP 1 Score: 152.525 bits (384), Expect = 2.747e-41
Identity = 81/184 (44.02%), Postives = 106/184 (57.61%), Query Frame = 0
Query:   17 NKLQATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQIDSDYKRQDG-EHSLHHGRWDWHSYILKGQIQP---LFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKEGMKEG 196
             + +A ++RS +  T A   PRPSP+LF FPG+TS P H+     W R LE    TI+ EY+ LK+ Q  SDY  Q G EHSLH G+WDW SY+ KG+ +P    F E CP T+ LL         M   PF+++FFS+L   S I  H+ P N+RLRCH PL VP G      ++ +   KEG
Sbjct:   15 TRAKAALLRSALCLTTAK--PRPSPTLFMFPGLTSAPFHNPRDFDWTRALEANVDTIRTEYLALKAHQKTSDYDVQGGQEHSLHQGQWDWFSYVTKGK-KPNGASFEEHCPTTAALLTSIPG---FMTSVPFAYAFFSSLQPGSSIKAHSAPCNIRLRCHFPLFVPPGCGIRVADQTRS-WKEG 191          
BLAST of EWM28449.1 vs. NCBI_GenBank
Match: gi|567972124|gb|ETK97115.1| (hypothetical protein, variant [Phytophthora parasitica] >gi|568000447|gb|ETL50465.1| hypothetical protein, variant [Phytophthora parasitica] >gi|570338153|gb|ETO86101.1| hypothetical protein, variant [Phytophthora parasitica P1976] >gi|570998988|gb|ETP55091.1| hypothetical protein, variant [Phytophthora parasitica P10297])

HSP 1 Score: 151.754 bits (382), Expect = 5.218e-41
Identity = 76/184 (41.30%), Postives = 106/184 (57.61%), Query Frame = 0
Query:   20 QATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQID------------SDYKRQDGEHSLHHGRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKE 191
            +A ++RS +  T A   PRPSP+LF FPG+ S P HD ++  W + LE    TI  EY+ LK ++ +            SDYK  D EH LH G+WDW SY+ +G+ Q  FA  CP+T  +L++       M   PF++SFFS L  +S I  H+ P N+RLRCH PLIVP+G      +E ++
Sbjct:    4 RAALLRSALCLTTAK--PRPSPTLFCFPGLESAPWHDTSKFEWVKHLEANRETITNEYLALKEQRDELAKNSEEVANEASDYKVYDKEHQLHQGKWDWLSYVTQGRRQADFAVQCPKTVEILEDIPG---FMAGLPFAYSFFSILKPESSIKAHSAPCNIRLRCHFPLIVPEGCGIRVGDETRQ 182          
BLAST of EWM28449.1 vs. NCBI_GenBank
Match: gi|570962163|gb|ETP27142.1| (hypothetical protein, variant [Phytophthora parasitica CJ01A1])

HSP 1 Score: 151.369 bits (381), Expect = 6.393e-41
Identity = 76/184 (41.30%), Postives = 106/184 (57.61%), Query Frame = 0
Query:   20 QATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQID------------SDYKRQDGEHSLHHGRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKE 191
            +A ++RS +  T A   PRPSP+LF FPG+ S P HD ++  W + LE    TI  EY+ LK+++              SDYK  D EH LH G+WDW SY+ +G+ Q  FA  CP+T  +L++       M   PF++SFFS L  +S I  H+ P N+RLRCH PLIVP+G      +E ++
Sbjct:    4 RAALLRSALCLTTAK--PRPSPTLFCFPGLESAPWHDTSKFEWVKHLEANRETITNEYLALKAQRDALAKNSEEVANEASDYKVYDKEHQLHQGKWDWLSYVTQGRRQADFAVQCPKTVEILEDIPG---FMAGLPFAYSFFSILKPESSIKAHSAPCNIRLRCHFPLIVPEGCGIRVGDETRQ 182          
BLAST of EWM28449.1 vs. NCBI_GenBank
Match: gi|675162009|ref|XP_008889889.1| (hypothetical protein, variant [Phytophthora parasitica INRA-310] >gi|568109124|gb|ETN23731.1| hypothetical protein, variant [Phytophthora parasitica INRA-310])

HSP 1 Score: 151.369 bits (381), Expect = 6.601e-41
Identity = 76/184 (41.30%), Postives = 106/184 (57.61%), Query Frame = 0
Query:   20 QATIIRSGMLKTNAGVLPRPSPSLFYFPGITSKPVHDATQLHWARVLEEEYATIKQEYMGLKSRQID------------SDYKRQDGEHSLHHGRWDWHSYILKGQIQPLFAELCPETSRLLQEFNRQSFLMRDTPFSFSFFSTLHGQSVIAPHTGPSNLRLRCHLPLIVPQGGKTEANEEKKE 191
            +A ++RS +  T A   PRPSP+LF FPG+ S P HD ++  W + LE    TI  EY+ LK+++              SDYK  D EH LH G+WDW SY+ +G+ Q  FA  CP+T  +L++       M   PF++SFFS L  +S I  H+ P N+RLRCH PLIVP+G      +E ++
Sbjct:    4 RAALLRSALCLTTAK--PRPSPTLFCFPGLESAPWHDTSKFEWVKHLEANRETITNEYLALKAQRDALAKNSEEAANEASDYKVYDKEHQLHQGKWDWLSYVTQGRRQADFAVQCPKTVEILEDIPG---FMAGLPFAYSFFSILKPESSIKAHSAPCNIRLRCHFPLIVPEGCGIRVGDETRQ 182          
The following BLAST results are available for this feature:
BLAST of EWM28449.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585110851|gb|EWM28449.1|0.000e+0100.00aspartyl asparaginyl beta-hydroxylase [Nannochloro... [more]
gi|585110852|gb|EWM28450.1|2.707e-171100.00aspartyl asparaginyl beta-hydroxylase [Nannochloro... [more]
gi|1173943950|gb|OQR92744.1|2.636e-4344.63aspartyl/Asparaginyl beta-hydroxylase [Achlya hypo... [more]
gi|1173958312|gb|OQS04692.1|3.800e-4343.89aspartyl/Asparaginyl beta-hydroxylase [Thraustothe... [more]
gi|813157761|ref|XP_012202229.1|1.128e-4244.07hypothetical protein SPRG_07847 [Saprolegnia paras... [more]
gi|669143896|ref|XP_008607510.1|2.260e-4243.50hypothetical protein SDRG_03651 [Saprolegnia dicli... [more]
gi|698822973|ref|XP_009844515.1|2.747e-4144.02hypothetical protein, variant [Aphanomyces astaci]... [more]
gi|567972124|gb|ETK97115.1|5.218e-4141.30hypothetical protein, variant [Phytophthora parasi... [more]
gi|570962163|gb|ETP27142.1|6.393e-4141.30hypothetical protein, variant [Phytophthora parasi... [more]
gi|675162009|ref|XP_008889889.1|6.601e-4141.30hypothetical protein, variant [Phytophthora parasi... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna2233rna2233Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications