EWM28606.1, cds2127 (CDS) Nannochloropsis gaditana

BLAST of EWM28606.1 vs. NCBI_GenBank
Match: gi|585111024|gb|EWM28606.1| (ppgpp synthetase [Nannochloropsis gaditana])

HSP 1 Score: 1729.53 bits (4478), Expect = 0.000e+0
Identity = 845/845 (100.00%), Postives = 845/845 (100.00%), Query Frame = 0

Query:    1 MTLCTDRPWGQPAELLGFPAPAGSPARLKLPDRQPSGNMVTNFKKKIRRSLQLLLLIPPLYRVNGWVSGSIQSLERHHHHAPRRRQPSANKVLSSPPTLHNIKAAVTPAPTDGSRNEYEDVRDDEDATELQKQITIELTDWVSEGEELWQEAFSTPRDEKRHRGKSEPGDNGMGHSTRPSNGLFTTVLGFRSAAHDDLSHGQAAHQLPVRLQREVNLVWYEIQRGDRMAYLRAEERDRVKQQLVVLAFFRTLFDHPSQVLNTQSAEACFQQADVNRDGQLSFEEFLRWYTASYDDQGPKSPEGRDSSEEEGGSTEETAGALVPFGQGAAARRLEGLSMTEVLRTFLAQKEHVSEAGPEADNKSEEQVLADLDRVIETTQVLLELEADVETVLAALATYFLGLDAEDSEDLTEKLAVVRSQCGPVVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEADVQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTGPRGRFLRGQLARKKNALPAWSTATTVALGDGASEGYGERGNVGGRNSDGTQRALGPAGATPVFSSPLPSASRLELEAWLCHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTAEIQIRTAGMHAEAEDGIASHALYKAELQTRDQVEDFQEQLGAARAATPAGEAAAKTETGKPALLLPASASSASWAVTVALPGEASSGG 845
            MTLCTDRPWGQPAELLGFPAPAGSPARLKLPDRQPSGNMVTNFKKKIRRSLQLLLLIPPLYRVNGWVSGSIQSLERHHHHAPRRRQPSANKVLSSPPTLHNIKAAVTPAPTDGSRNEYEDVRDDEDATELQKQITIELTDWVSEGEELWQEAFSTPRDEKRHRGKSEPGDNGMGHSTRPSNGLFTTVLGFRSAAHDDLSHGQAAHQLPVRLQREVNLVWYEIQRGDRMAYLRAEERDRVKQQLVVLAFFRTLFDHPSQVLNTQSAEACFQQADVNRDGQLSFEEFLRWYTASYDDQGPKSPEGRDSSEEEGGSTEETAGALVPFGQGAAARRLEGLSMTEVLRTFLAQKEHVSEAGPEADNKSEEQVLADLDRVIETTQVLLELEADVETVLAALATYFLGLDAEDSEDLTEKLAVVRSQCGPVVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEADVQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTGPRGRFLRGQLARKKNALPAWSTATTVALGDGASEGYGERGNVGGRNSDGTQRALGPAGATPVFSSPLPSASRLELEAWLCHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTAEIQIRTAGMHAEAEDGIASHALYKAELQTRDQVEDFQEQLGAARAATPAGEAAAKTETGKPALLLPASASSASWAVTVALPGEASSGG
Sbjct:    1 MTLCTDRPWGQPAELLGFPAPAGSPARLKLPDRQPSGNMVTNFKKKIRRSLQLLLLIPPLYRVNGWVSGSIQSLERHHHHAPRRRQPSANKVLSSPPTLHNIKAAVTPAPTDGSRNEYEDVRDDEDATELQKQITIELTDWVSEGEELWQEAFSTPRDEKRHRGKSEPGDNGMGHSTRPSNGLFTTVLGFRSAAHDDLSHGQAAHQLPVRLQREVNLVWYEIQRGDRMAYLRAEERDRVKQQLVVLAFFRTLFDHPSQVLNTQSAEACFQQADVNRDGQLSFEEFLRWYTASYDDQGPKSPEGRDSSEEEGGSTEETAGALVPFGQGAAARRLEGLSMTEVLRTFLAQKEHVSEAGPEADNKSEEQVLADLDRVIETTQVLLELEADVETVLAALATYFLGLDAEDSEDLTEKLAVVRSQCGPVVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEADVQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTGPRGRFLRGQLARKKNALPAWSTATTVALGDGASEGYGERGNVGGRNSDGTQRALGPAGATPVFSSPLPSASRLELEAWLCHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTAEIQIRTAGMHAEAEDGIASHALYKAELQTRDQVEDFQEQLGAARAATPAGEAAAKTETGKPALLLPASASSASWAVTVALPGEASSGG 845          

BLAST of EWM28606.1 vs. NCBI_GenBank
Match: gi|298713995|emb|CBJ27227.1| (GTP diphosphokinase [Ectocarpus siliculosus])

HSP 1 Score: 218.779 bits (556), Expect = 6.536e-56
Identity = 149/421 (35.39%), Postives = 225/421 (53.44%), Query Frame = 0

Query:  424 VVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEADVQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTGPRGRFLRGQLARKKNALPAWSTATTVALGDGASEGYGERGNVGGRNSDGTQ-RALGPAGATPVFSSPLPSASRLELEAWLCHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTAEIQIRTAGMHAEAEDGIASHALYKAELQTRDQVEDFQEQLGAARAATPAGEAAAKTETGKPALLLPASASSASWAVTVALPGEASS 843
            V H++     R G  F  SP  + +   P     +LDLD  +A+L+R +LV+ + DARA VVHMA L   LR+  + P H RH LALE+LQLYVPV++ALGLGS  +E+EEL Y+ +FP++Y + A WH +        +   K ++   + A  + +   +  Y + GR K++      V+R++K  +++HD++G R+I+ P    G R     G   R ++           A G   +E   E  N G R   G   R  GP  A  VF+         + ++ L H V+ V+  L+EEVPGR+KNYVD PK NGYRS+HT V +   G   E Q+RTA MH  AE G ASH+LYK +L   ++   F+ ++     +    +  +K+++G+ A  L  +A   S A  V+  G A++
Sbjct:  503 VSHRMHGRRARAGSAFTCSPP-QSFGVKP-----VLDLDDHHAKLMRDYLVHSSRDARAVVVHMADLTCRLRDPSKTPAHTRHTLALEALQLYVPVSNALGLGSSFRELEELGYKTIFPQTYSNMALWHHDVMGKSHDIVREVKTRMLDALHAR-EDIAIRVHSYRLEGRTKAL------VFRQNKRADDVHDIIGFRIIVAPKR-PGTRASSATGGAGRTRS-----------ATGGNTAE---EAENAGARKRPGDDRRRGGPRPARSVFTVKTFPPPYRDADSRLLHDVYEVLVGLFEEVPGRFKNYVDFPKKNGYRSVHTTV-VHPTGIKMEFQVRTALMHVAAEGGSASHSLYKGDLDNPEEASLFRSKMTPNILSPAHRQLQSKSKSGEAATALQPTADGGSPAANVSEKGPAAA 894          

HSP 2 Score: 75.0998 bits (183), Expect = 3.946e-10
Identity = 36/97 (37.11%), Postives = 59/97 (60.82%), Query Frame = 0

Query:  208 PVRLQREVNLVWYEIQRGDRMAYLRAEERDRVKQQLVVLAFFRTLFDHPSQVLNTQSAEACFQQADVNRDGQLSFEEFLRWYTAS--YDDQGPKSPE 302
            P R Q+ +  +W  + RG+++ YL  E + +VKQ L VLA +  L +HP Q    ++ +  F + D N DG++SF+EF+RWY+ S   D++  + PE
Sbjct:  125 PFRFQQLIQQMWATLSRGNQLEYLGKEGKAKVKQALSVLAAYSALREHPLQT-GVENPQQAFSEMDFNSDGEVSFDEFVRWYSMSTALDEKPQQLPE 220          

BLAST of EWM28606.1 vs. NCBI_GenBank
Match: gi|551663923|ref|XP_005834725.1| (hypothetical protein GUITHDRAFT_41256, partial [Guillardia theta CCMP2712] >gi|428178872|gb|EKX47745.1| hypothetical protein GUITHDRAFT_41256, partial [Guillardia theta CCMP2712])

HSP 1 Score: 116.701 bits (291), Expect = 4.774e-25
Identity = 73/208 (35.10%), Postives = 114/208 (54.81%), Query Frame = 0

Query:  417 VRSQCGPVVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEADVQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVIL 624
            VR + GPVV  +V DS  L  L    P+                LD   ++ +R+++V  + D RA +V +A  L+ALR     P++ +H+ ALESLQ++ P+A ALG+G  L E+E+LS+RALFP+SY S   W     ++    +  AK  +   +  +   L   +E Y I  R KSV S FKK++R +K  E++ D+  +RV++
Sbjct:   21 VRDRFGPVVGSMVEDSVWLSQL----PDRVGL------------LDDDGSRFLREYMVAASRDHRAVIVMLADRLNALRKIEEAPLYDQHLKALESLQVFSPLAHALGVGKWLWELEDLSFRALFPDSYTSVEQWQLELWEVSSNMMEQAKVDVLKALNEN-DFLLEHVERYAITSRRKSVFSTFKKMFRSNKKLEDVKDVFAMRVVI 211          

HSP 2 Score: 64.6994 bits (156), Expect = 1.501e-7
Identity = 34/60 (56.67%), Postives = 40/60 (66.67%), Query Frame = 0

Query:  722 WEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTAEIQIRTAGMHAEAEDGIASHALYK 781
            WEE  GR+K+YV  PKPNGY+S+HT +         E+QIRT  MHA AE G ASH LYK
Sbjct:  242 WEEPRGRFKDYVTHPKPNGYQSIHTTLD-SPLKLPLEVQIRTEEMHAAAEFGDASHNLYK 300          

BLAST of EWM28606.1 vs. NCBI_GenBank
Match: gi|302790548|ref|XP_002977041.1| (hypothetical protein SELMODRAFT_21858, partial [Selaginella moellendorffii] >gi|300155017|gb|EFJ21650.1| hypothetical protein SELMODRAFT_21858, partial [Selaginella moellendorffii])

HSP 1 Score: 118.242 bits (295), Expect = 2.337e-24
Identity = 66/174 (37.93%), Postives = 110/174 (63.22%), Query Frame = 0

Query:  460 DLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEAD--VQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTG 631
            +LD  NA ++R   +  + D RA VV +A  L+ +R+   +P   + + ALE++QLY P+A ALGLG+  +E+E++++R LFP+SY+    W ++    G+  +  + K++ A +EAD  +QG+   +E  ++ GR KS  S  KK+ R  + P++++D+LGLRVILFP  G G
Sbjct:  119 NLDDENANMLRDFSLAYH-DIRAMVVELASRLNEMRHVDCLPRLRQQMAALENMQLYAPLAHALGLGTLSQELEDMAFRILFPDSYQCVEDWLQSHWGDGEAIIAESIKQLKAALEADSVLQGM---VESVKVTGRRKSRFSTMKKLLRDGRGPDQVYDILGLRVILFPKTGAG 288          

HSP 2 Score: 90.1225 bits (222), Expect = 3.178e-15
Identity = 46/97 (47.42%), Postives = 63/97 (64.95%), Query Frame = 0

Query:  711 CHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTA---EIQIRTAGMHAEAEDGIASHALYKAELQTRDQVEDFQE-QLGAARAA 803
            C++   +V ++W+EVPGR K+Y+  PK NGY SLH  V+L + G  +   E+QIRT+ MHA A  G +SHALYK  L    QVE+F+   L AA+ A
Sbjct:  296 CYRAQKIVTNMWKEVPGRMKDYIAKPKLNGYSSLHLAVFLTKYGPWSLPMELQIRTSAMHAHAVAGGSSHALYKGGLTDPRQVENFKAVMLAAAKTA 392          

BLAST of EWM28606.1 vs. NCBI_GenBank
Match: gi|302763085|ref|XP_002964964.1| (hypothetical protein SELMODRAFT_83164 [Selaginella moellendorffii] >gi|300167197|gb|EFJ33802.1| hypothetical protein SELMODRAFT_83164 [Selaginella moellendorffii])

HSP 1 Score: 118.242 bits (295), Expect = 7.194e-24
Identity = 82/263 (31.18%), Postives = 139/263 (52.85%), Query Frame = 0

Query:  371 LDRVIETTQVLLELEADVETVLAALATYFLGLDAEDSEDLTEKLAVVRSQCGPVVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEAD--VQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTG 631
            L R +     L +L  D E + + +        A ++E L+  L  V+ + G     ++ DS R+  L                   + +LD  NA ++R   +  + D RA VV +A  L  +R+   +P   + + ALE++QLY P+A ALGLG+  +E+E++++R LFP+SY+    W ++    G+  +  + K++ A +EAD  +QG+   +E  ++ GR KS  S  KK+ R  + P++++D+LGLRVILFP  G G
Sbjct:  178 LSRALAIAYTLADLHMDAEVIASGILRV-----AMETEVLS--LTDVQRELGAGAFHLLDDSLRVNQL----------------ACCLDNLDDENANMLRDFSLAYH-DIRAMVVELASRLDEMRHVDCLPRLRQQMAALENMQLYAPLAHALGLGTLSQELEDMAFRILFPDSYQCVEDWLQSHWGDGEAIIAESIKQLKAALEADSVLQGM---VESVKVTGRRKSRFSTMKKLLRDGRGPDQVYDILGLRVILFPKTGAG 413          

HSP 2 Score: 90.1225 bits (222), Expect = 5.957e-15
Identity = 46/97 (47.42%), Postives = 63/97 (64.95%), Query Frame = 0

Query:  711 CHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTA---EIQIRTAGMHAEAEDGIASHALYKAELQTRDQVEDFQE-QLGAARAA 803
            C++   +V ++W+EVPGR K+Y+  PK NGY SLH  V+L + G  +   E+QIRT+ MHA A  G +SHALYK  L    QVE+F+   L AA+ A
Sbjct:  421 CYRAQKIVTNMWKEVPGRMKDYIAKPKLNGYSSLHLAVFLTKYGPWSLPMELQIRTSAMHAHAVAGGSSHALYKGGLTDPRQVENFKAVMLAAAKTA 517          

BLAST of EWM28606.1 vs. NCBI_GenBank
Match: gi|763102389|ref|WP_043982777.1| (bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Meiothermus ruber])

HSP 1 Score: 118.242 bits (295), Expect = 1.358e-23
Identity = 81/264 (30.68%), Postives = 138/264 (52.27%), Query Frame = 0

Query:  375 IETTQVLLELEADVETVLAALATYFLGLDAEDSEDLTEKLAVVRSQCGPVVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEADVQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTGPRGRFLR 638
            +   Q+L EL  DVET+LA       GL  +  ED   +   +  + G  V +IV   T++  L++ +   ++ +            + ++A+ +RQ  +    D R  +V +A  LH LR    MP H +  +A E+L++Y P+A  LG+G    E+E+LS+R L PESY++     K+     + A+  A++K+   +  D   L++ ++ +E+ GR K + S +KK+ R+ K  E+++D+L LRVIL P  G  P    LR
Sbjct:   69 VAVAQILAELGMDVETLLA-------GLLHDTIEDTQVRPEEIEERFGAAVRKIVEGETKVSKLYKLAHAAQEDKHA----------EEKHAEDLRQMFIAMAEDVRIIIVKLADRLHNLRTLEFMPPHKQQRIARETLEIYAPLAHRLGIGQVKLELEDLSFRYLDPESYQALERRLKSHRAEREAAVQAAREKLEQALARDFI-LKQSIKKFEVTGRTKHLFSIWKKMEREGKTIEQIYDLLALRVILDPRQGQDPEETSLR 314          

HSP 2 Score: 83.1889 bits (204), Expect = 1.044e-12
Identity = 44/105 (41.90%), Postives = 65/105 (61.90%), Query Frame = 0

Query:  707 EAWLCHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTAEIQIRTAGMHAEAEDGIASHALYK------AELQTR----DQVEDFQEQLGAAR 801
            E  +C+ V  +V +LW+ +PGR K+Y+  PKPNGY+SLHT V  +  G   E+QIRT  MH  AE G+A+H LYK       EL+ R      ++D+Q++  ++R
Sbjct:  315 EKQVCYHVLGLVHALWQPIPGRVKDYIAVPKPNGYQSLHTTVIAV-NGLPLEVQIRTREMHRVAEFGVAAHWLYKEGVTDPEELRRRVGWLKSIQDWQQEYSSSR 418          

BLAST of EWM28606.1 vs. NCBI_GenBank
Match: gi|568062194|ref|WP_024050601.1| (bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Meiothermus ruber])

HSP 1 Score: 117.857 bits (294), Expect = 1.616e-23
Identity = 83/264 (31.44%), Postives = 139/264 (52.65%), Query Frame = 0

Query:  375 IETTQVLLELEADVETVLAALATYFLGLDAEDSEDLTEKLAVVRSQCGPVVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEADVQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTGPRGRFLR 638
            +   Q+L EL  DVET+LA L    L    ED+E   E    +  + G  V +IV   T++  L++ +   ++ +            + ++A+ +RQ  +    D R  +V +A  LH LR    MP H +  +A E+L++Y P+A  LG+G    E+E+LS+R L PE+Y++     K+     + A+  AK+K+   +  D   L++ ++ +E+ GR K + S +KK+ R+ K  E+++D+L LRVIL P  G  P    LR
Sbjct:   69 VAVAQILAELGLDVETLLAGL----LHDTIEDTEVRPED---IEERFGAAVRKIVEGETKVSKLYKLAHAAQEDKHA----------EEKHAEDLRQMFIAMAEDVRIIIVKLADRLHNLRTLEFMPPHKQQRIARETLEIYAPLAHRLGIGQVKLELEDLSFRYLDPENYQALERRLKSHRAEREAAVQAAKEKLEQALARDFI-LKQSIKKFEVTGRTKHLFSIWKKMEREGKTIEQIYDLLALRVILDPRQGPDPEENSLR 314          

HSP 2 Score: 82.0333 bits (201), Expect = 2.468e-12
Identity = 43/105 (40.95%), Postives = 65/105 (61.90%), Query Frame = 0

Query:  707 EAWLCHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTAEIQIRTAGMHAEAEDGIASHALYK------AELQTR----DQVEDFQEQLGAAR 801
            E  +C+ V  +V +LW+ +PGR K+Y+  PKPNGY+SLHT V  +  G   E+QIRT  MH  AE G+A+H LYK       +L+ R      ++D+Q++  ++R
Sbjct:  315 EKQVCYHVLGLVHALWQPIPGRVKDYIAVPKPNGYQSLHTTVIAV-NGLPLEVQIRTREMHRVAEFGVAAHWLYKEGVTDPEDLRRRVGWLKSIQDWQQEYSSSR 418          

BLAST of EWM28606.1 vs. NCBI_GenBank
Match: gi|916742791|ref|WP_051349847.1| (bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Meiothermus taiwanensis])

HSP 1 Score: 117.857 bits (294), Expect = 1.891e-23
Identity = 81/264 (30.68%), Postives = 138/264 (52.27%), Query Frame = 0

Query:  375 IETTQVLLELEADVETVLAALATYFLGLDAEDSEDLTEKLAVVRSQCGPVVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEADVQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTGPRGRFLR 638
            +   Q+L EL  DVET+LA       GL  +  ED   +   +  + G  V +IV   T++  L++ +   ++ +            + ++A+ +RQ  +    D R  +V +A  LH LR    MP H +  +A E+L++Y P+A  LG+G    E+E+LS+R L PESY++     K+     + A+  A++K+   +  D   L++ ++ +E+ GR K + S +KK+ R+ K  E+++D+L LRVIL P  G  P    LR
Sbjct:   69 VAVAQILAELGMDVETLLA-------GLLHDTIEDTQVRPEEIEERFGAAVRKIVEGETKVSKLYKLAHAAQEDKHA----------EEKHAEDLRQMFIAIAEDVRIIIVKLADRLHNLRTLEFMPPHKQQRIARETLEIYAPLAHRLGIGQVKLELEDLSFRYLDPESYQALERRLKSHRAEREAAVQAAREKLEQALARDFI-LKQSIKKFEVTGRTKHLFSIWKKMEREGKTIEQIYDLLALRVILDPRQGQDPEETSLR 314          

HSP 2 Score: 83.1889 bits (204), Expect = 1.044e-12
Identity = 44/105 (41.90%), Postives = 65/105 (61.90%), Query Frame = 0

Query:  707 EAWLCHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTAEIQIRTAGMHAEAEDGIASHALYK------AELQTR----DQVEDFQEQLGAAR 801
            E  +C+ V  +V +LW+ +PGR K+Y+  PKPNGY+SLHT V  +  G   E+QIRT  MH  AE G+A+H LYK       EL+ R      ++D+Q++  ++R
Sbjct:  315 EKQVCYHVLGLVHALWQPIPGRVKDYIAVPKPNGYQSLHTTVIAV-NGLPLEVQIRTREMHRVAEFGVAAHWLYKEGVTDPEELRRRVGWLKSIQDWQQEYSSSR 418          

BLAST of EWM28606.1 vs. NCBI_GenBank
Match: gi|756399682|gb|KIQ54470.1| (GTP pyrophosphokinase [Meiothermus ruber])

HSP 1 Score: 117.472 bits (293), Expect = 1.928e-23
Identity = 81/264 (30.68%), Postives = 138/264 (52.27%), Query Frame = 0

Query:  375 IETTQVLLELEADVETVLAALATYFLGLDAEDSEDLTEKLAVVRSQCGPVVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEADVQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTGPRGRFLR 638
            +   Q+L EL  DVET+LA       GL  +  ED   +   +  + G  V +IV   T++  L++ +   ++ +            + ++A+ +RQ  +    D R  +V +A  LH LR    MP H +  +A E+L++Y P+A  LG+G    E+E+LS+R L PESY++     K+     + A+  A++K+   +  D   L++ ++ +E+ GR K + S +KK+ R+ K  E+++D+L LRVIL P  G  P    LR
Sbjct:   47 VAVAQILAELGMDVETLLA-------GLLHDTIEDTQVRPEEIEERFGAAVRKIVEGETKVSKLYKLAHAAQEDKHA----------EEKHAEDLRQMFIAMAEDVRIIIVKLADRLHNLRTLEFMPPHKQQRIARETLEIYAPLAHRLGIGQVKLELEDLSFRYLDPESYQALERRLKSHRAEREAAVQAAREKLEQALARDFI-LKQSIKKFEVTGRTKHLFSIWKKMEREGKTIEQIYDLLALRVILDPRQGQDPEETSLR 292          

HSP 2 Score: 83.1889 bits (204), Expect = 1.099e-12
Identity = 44/105 (41.90%), Postives = 65/105 (61.90%), Query Frame = 0

Query:  707 EAWLCHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTAEIQIRTAGMHAEAEDGIASHALYK------AELQTR----DQVEDFQEQLGAAR 801
            E  +C+ V  +V +LW+ +PGR K+Y+  PKPNGY+SLHT V  +  G   E+QIRT  MH  AE G+A+H LYK       EL+ R      ++D+Q++  ++R
Sbjct:  293 EKQVCYHVLGLVHALWQPIPGRVKDYIAVPKPNGYQSLHTTVIAV-NGLPLEVQIRTREMHRVAEFGVAAHWLYKEGVTDPEELRRRVGWLKSIQDWQQEYSSSR 396          

BLAST of EWM28606.1 vs. NCBI_GenBank
Match: gi|888642486|dbj|GAO76464.1| ((p)ppGpp synthetase I SpoT/RelA [Meiothermus ruber H328])

HSP 1 Score: 117.087 bits (292), Expect = 2.640e-23
Identity = 83/264 (31.44%), Postives = 139/264 (52.65%), Query Frame = 0

Query:  375 IETTQVLLELEADVETVLAALATYFLGLDAEDSEDLTEKLAVVRSQCGPVVHQIVRDSTRLGGLFQASPETRQYRRNPREYYTMLDLDHRNAQLVRQHLVNENSDARAFVVHMAGLLHALRNQYRMPVHARHVLALESLQLYVPVASALGLGSRLKEMEELSYRALFPESYESFASWHKNFADLGQVALVIAKKKIAARIEADVQGLRRFLEGYEIHGRVKSVVSAFKKVYRKDKLPEELHDMLGLRVILFPSLGTGPRGRFLR 638
            +   Q+L EL  DVET+LA L    L    ED+E   E    +  + G  V +IV   T++  L++ +   ++ +            + ++A+ +RQ  +    D R  +V +A  LH LR    MP H +  +A E+L++Y P+A  LG+G    E+E+LS+R L PE+Y++     K+     + A+  AK+K+   +  D   L++ ++ +E+ GR K + S +KK+ R+ K  E+++D+L LRVIL P  G  P    LR
Sbjct:   47 VAVAQILAELGLDVETLLAGL----LHDTIEDTEVRPED---IEERFGAAVRKIVEGETKVSKLYKLAHAAQEDKHA----------EEKHAEDLRQMFIAMAEDVRIIIVKLADRLHNLRTLEFMPPHKQQRIARETLEIYAPLAHRLGIGQVKLELEDLSFRYLDPENYQALERRLKSHRAEREAAVQAAKEKLEQALARDFI-LKQSIKKFEVTGRTKHLFSIWKKMEREGKTIEQIYDLLALRVILDPRQGPDPEENSLR 292          

HSP 2 Score: 82.0333 bits (201), Expect = 2.668e-12
Identity = 46/115 (40.00%), Postives = 69/115 (60.00%), Query Frame = 0

Query:  697 PLPSASRLELEAWLCHQVHAVVASLWEEVPGRYKNYVDAPKPNGYRSLHTGVYLMEGGGTAEIQIRTAGMHAEAEDGIASHALYK------AELQTR----DQVEDFQEQLGAAR 801
            P P  + L  E  +C+ V  +V +LW+ +PGR K+Y+  PKPNGY+SLHT V  +  G   E+QIRT  MH  AE G+A+H LYK       +L+ R      ++D+Q++  ++R
Sbjct:  284 PDPEENSLR-EKQVCYHVLGLVHALWQPIPGRVKDYIAVPKPNGYQSLHTTVIAV-NGLPLEVQIRTREMHRVAEFGVAAHWLYKEGVTDPEDLRRRVGWLKSIQDWQQEYSSSR 396