EWM28618.1, cds2085 (CDS) Nannochloropsis gaditana

BLAST of EWM28618.1 vs. NCBI_GenBank
Match: gi|585111035|gb|EWM28617.1| (amidase protein [Nannochloropsis gaditana] >gi|585111036|gb|EWM28618.1| amidase protein [Nannochloropsis gaditana])

HSP 1 Score: 1279.62 bits (3310), Expect = 0.000e+0
Identity = 620/620 (100.00%), Postives = 620/620 (100.00%), Query Frame = 0

Query:    1 MFWWLSTAAERLQRKARAKRAEFEAKWAQPLPPLAPELDAAKETKIALELSIEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGESDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFRPASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIVMPSKSGKMGMQAIRATAGPLANRVEDVALVCQAWWVNKMWARDPYVVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEGGLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTALYLTGEKRRRFLVKNMRRDGLATRTYWQYCEELQNYQEAWTRMMQTQKLDGFLCPSVALPAFPHGWSSHLESSCSYNLFLNLLHYPAGVIPVTQVRADEAVYPISDLPRMQRDSIARAAAIAMQGSEALPVSVQVAFPPFQDEMCLHVMKDLQDAVGFTALPPYAKRSVFN 620
            MFWWLSTAAERLQRKARAKRAEFEAKWAQPLPPLAPELDAAKETKIALELSIEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGESDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFRPASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIVMPSKSGKMGMQAIRATAGPLANRVEDVALVCQAWWVNKMWARDPYVVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEGGLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTALYLTGEKRRRFLVKNMRRDGLATRTYWQYCEELQNYQEAWTRMMQTQKLDGFLCPSVALPAFPHGWSSHLESSCSYNLFLNLLHYPAGVIPVTQVRADEAVYPISDLPRMQRDSIARAAAIAMQGSEALPVSVQVAFPPFQDEMCLHVMKDLQDAVGFTALPPYAKRSVFN
Sbjct:    1 MFWWLSTAAERLQRKARAKRAEFEAKWAQPLPPLAPELDAAKETKIALELSIEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGESDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFRPASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIVMPSKSGKMGMQAIRATAGPLANRVEDVALVCQAWWVNKMWARDPYVVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEGGLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTALYLTGEKRRRFLVKNMRRDGLATRTYWQYCEELQNYQEAWTRMMQTQKLDGFLCPSVALPAFPHGWSSHLESSCSYNLFLNLLHYPAGVIPVTQVRADEAVYPISDLPRMQRDSIARAAAIAMQGSEALPVSVQVAFPPFQDEMCLHVMKDLQDAVGFTALPPYAKRSVFN 620          

BLAST of EWM28618.1 vs. NCBI_GenBank
Match: gi|219119326|ref|XP_002180426.1| (predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1] >gi|217407899|gb|EEC47834.1| predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 372.859 bits (956), Expect = 7.527e-120
Identity = 208/492 (42.28%), Postives = 286/492 (58.13%), Query Frame = 0

Query:  121 LEGIPLSVKDHIDVKGTDSTTGAACRCFRPASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIVMPSKSGKMGMQA-IRATAGPLANRVEDVALVCQAWWVNKMWARDPYVVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEGGLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTALYLTGEKRRRFLVKNMRRDGLATRTYWQYCEELQNYQEAWTRMMQTQKLDGFLCPSVALPAFPHGWSSHLESSCSYNLFLNLLHYPAGVIPVTQVRADEAVYPISDLPRMQRDSIARAAAIAMQGSEALPVSVQVAFPPFQDEMCLHVMKDLQDAVGFTALP 611
            L G+P+SVK+H+ ++G+ ST G ACR  +  +KD+L+V+++R AGAI +C  NVPQ +MLPET N IWG + +PW+L RS GGSSGG+AALVA R   + IG+D+ GS R PA F G VGFKPT  RVSGKG +   K+ + G  A I    GPLA  V+D A   +A  V +M+  D  V PLPFD  +Y                           Q +A      K+++G+F +D +FEPC   KRAVREA  AL   G +  V  +L T  DGW +  L   I  AEG  RS+++ALEGE MI  Y  +  A+  P+ L+P++   +    +KRR  L+K  R  G+     WQ   ++   ++ W   ++   LD  + P++ +PA  HG S  L +SCSY    NLL +P+G +PVT VRADEA Y   D+P  QRD I+R  A  MQGSE +P+SV V  P ++DE CL VMK+++  + F   P
Sbjct:    2 LYGVPISVKEHLALRGSYSTGGLACRLNQKDTKDSLIVQVIRSAGAIPMCSGNVPQIMMLPETYNRIWGRSRNPWDLCRSTGGSSGGDAALVAARCVPLAIGSDVAGSIRIPASFCGIVGFKPTAYRVSGKGNMKARKNNRSGTSAVIPVVCGPLARTVDDCAQFMKAVLVPEMFQGDSSVPPLPFDVDSY---------------------------QSKA------KLKIGYFDTDGWFEPCLTSKRAVREAIDALTKAG-HTCVPFKLPT--DGWISYGLLVAINAAEGNFRSFVEALEGEQMISEYDTLHQASNLPNLLKPVIMALI----DKRRGHLLKQGRNGGVPVWDLWQSVAKVLELRQKWDNAVREAGLDAIVHPAMPIPAIQHGLSGKLTASCSYMFLANLLQWPSGALPVTTVRADEAHYRRKDMPADQRDIISRIVAQVMQGSEGMPISVCVMSPSYRDETCLRVMKEIEKTIPFQEEP 453          

BLAST of EWM28618.1 vs. NCBI_GenBank
Match: gi|298711659|emb|CBJ32713.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 377.481 bits (968), Expect = 1.344e-119
Identity = 225/575 (39.13%), Postives = 328/575 (57.04%), Query Frame = 0

Query:   41 AKETKIALELSIEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGE--SDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFR-PASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIVMPSKSGKMGMQAIRATAGPLANRVEDVALVCQAWWVNKMWARDPYVVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEGGLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTAL-YLTGEKRRRFLVKNMRRDGLATRTYWQYCEELQNYQEAWTRMMQTQKLDGFLCPSVALPAFPHGWSSHLESSCSYNLFLNLLHYPAGVIPVTQVRADEAVYPISDLPRMQRDSIARAAAIAMQGSEALPVSVQVAFPPFQDEMCLHVMKDLQDAVGFTALP 611
            +++T+IA  L   +L+A M++GT S    V+   +RAR  G     N +T+  ++EA   AR+ D     GGG   +   G+L+G+P+S+KD + +KG  +T G AC+  + P+ +D L+V++LR  GAI   + NVPQC+MLPE+ N I+G + +PWNL R+ GGSSGGE +LVA R + + +G+D+GGS R P HF+G  G KPTP R+S KG+   S+  K G + I  T GP+AN V+D+ L   A    +MW  D  +  LP+D      G                                GR +++G+F +D +FEPC A  RAV EA + L + G +E+V         GW+  +LYY  M AEG ++ ++D+++GE M+P Y  +R  A FP+ LRP L   L  + G++RR  +V   R  GL+ R Y++   +  + +  W   +    LD  + P VALPA PHG +  L  + +Y    NLLH+PAGV+PVT VR DE  Y +  LP  QRD  AR A   M+GS  LPV VQV   PFQ+E+ LH M++++ AV F A+P
Sbjct:   71 SEDTRIA-RLRGTELMAAMRNGTLSCERAVAAFCRRARAVG-KVKTNGVTEEFYDEAVQAARKVDASGGGGGGGGPTATAGLLQGMPMSIKDAMHMKGALTTCGMACKAAKGPSKEDGLLVKVLRAHGAIPFVRGNVPQCMMLPESDNAIFGRSDNPWNLGRTPGGSSGGEGSLVASRSTPLALGSDVGGSIRIPCHFTGTCGLKPTPDRMSKKGMESLSEDNKGGQRIIHPTPGPIANCVDDLELAMTALCSVEMWDADKRIPRLPWDRTVAIDG-------------------------------PGRPLKVGFFTTDHWFEPCAAISRAVHEAAEGLRAAG-HEVVP--FEPPVSGWQIAKLYYGQMGAEGNMKRFVDSIQGERMLPSYAALRQLAGFPNCLRPALSWVLRNVLGDERRASIVGITRNKGLSVRQYYELVADTYDLRSKWEDAVTDAGLDAVIFPPVALPALPHGAAKDLTLAFTYMFVANLLHWPAGVVPVTLVRPDEQDYDVKRLPVNQRDGTARLARETMRGSAGLPVGVQVMTMPFQEELALHAMREVEKAVNFVAVP 609          

BLAST of EWM28618.1 vs. NCBI_GenBank
Match: gi|922852345|gb|KOO21040.1| (amidase signature enzyme [Chrysochromulina sp. CCMP291])

HSP 1 Score: 265.388 bits (677), Expect = 6.340e-79
Identity = 164/438 (37.44%), Postives = 242/438 (55.25%), Query Frame = 0

Query:   50 LSIEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGESDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFR-PASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIVMPSKSGKMGMQAIRATAGPLANRVEDVALVCQAWWVNKMWARDPYVVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEGGLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTAL-YLTGEKRRRFLVKNMRRDGLATRTYWQYCEELQNY 485
            ++  +L+  M  G  S+  V+     RA E      + A+T+  +EEA  EA+  D +R  G       G L G+P SVK+ I + G  ST GA CR    P+ + AL+V L + AGAI   +TNVPQ LMLPET N I+G T +P++L RS GGS+GGE AL+A RGS +G+GTD+GGS R PA  +G   FKPT  R+S KGI +P +  + G + +R+  GP+A  V D+ LV  A+    M+A D  +  +P+D A+    V   A+     G    +R G         S G + +R G+F++D +FEP  A KRAVR   +AL + G +E+VE    T  +  EA +LY  +++A+G  R ++D ++GE + P Y  +   AC P+++RP+L   + +  GE R+  LV  +         YWQ    +  Y
Sbjct:    1 MTATELLGAMSIGRLSAEYVMRCFIARAIEVD--ETLRAVTEQAYEEALAEAKACDLERAEG----KLRGPLHGLPFSVKEQIRMAGFQSTCGACCRLAEAPSEETALLVTLAKQAGAIPFARTNVPQLLMLPETFNAIYGTTCNPYDLGRSPGGSTGGECALIAARGSPLGLGTDVGGSVRIPAFMTGLCAFKPTVDRLSYKGITVPRRGNRSGQREVRSAPGPIARCVADLELVMHAFCQPAMYAADTTIPRMPWDRAS----VAHRAKGPTAKGPTSPSRRG---------SEGSKPLRFGYFVTDHWFEPAEACKRAVRGTAEALRAAG-HEVVEF---TPLEMAEAAKLYVALLSADGNFRGFLDGIDGEKLHPNYAFLCQVACVPNWIRPMLSAVMRHGLGEPRKADLV--LSGAAKTAHEYWQVITRVTAY 413          

BLAST of EWM28618.1 vs. NCBI_GenBank
Match: gi|551609070|ref|XP_005785768.1| (hypothetical protein EMIHUDRAFT_441645 [Emiliania huxleyi CCMP1516] >gi|485638968|gb|EOD33339.1| hypothetical protein EMIHUDRAFT_441645 [Emiliania huxleyi CCMP1516])

HSP 1 Score: 263.462 bits (672), Expect = 8.538e-76
Identity = 177/461 (38.39%), Postives = 239/461 (51.84%), Query Frame = 0

Query:   19 KRAEFEAKWAQPLPPLAPELDAAKETKIALELSIEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGESDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFRPASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIVMPSKSGK-MGMQAIRATAGPLANRVEDVALVCQAWWVNKMWARDPYVVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEGGLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTAL-YLTGEKRRRFLVKNMRRDGLATRTYWQ 477
            KR+   A W  P    +    AA+  +    L+   L+  M  G  +S  V+     RA   G     NA      +E F +A     +          +G L G+P+SVKD +D+ G DST G   RC  PA  D+L+V+LLRD GAI   ++N+PQCLMLPE++N IWG   +PW+ +R+ GGSSGGEAALVA R S +GIGTDIGGS R PAHF G  GFKPTP R+S +G+ +P    +  G  A+++TAGP    V D+ LV +AW                        G++       R G  E           E   P GR +R G   +D FF+P  A  RAV EA  ALE+ G +E+V +      + +EA  LY +++ AEGGL+ +ID LEGE ++PMY  +   A  P  LRP L  AL +L G+ R   LV   RR   +   YW+
Sbjct:   57 KRSRLVAPWPAP----SDHSPAAENMRHICSLNATALLEEMTAGRLTSVQVMRAFCSRALHVGERLDTNA------QECFADALAAAEEADSARAAGRPLGALHGLPVSVKDQLDMAGCDSTCGLQVRCGAPAEHDSLLVKLLRDQGAIPFVRSNLPQCLMLPESANRIWGAARNPWDASRTPGGSSGGEAALVAARASPLGIGTDIGGSIRIPAHFCGVFGFKPTPDRISNRGVAVPRPGNRPSGQIAVKSTAGPFGRSVADLRLVMRAWLAGG--------------------GMSDSDVTCPRAGWAEA----------EPGKPRGR-LRFGLLATDSFFDPSPACGRAVSEAAAALEAAG-HEVVAL----PGEFYEAALLYVSLLAAEGGLQGFIDGLEGEELLPMYSFLLRIATLPALLRPALRWALRHLLGQPRMADLVHAGRRH--SVHEYWR 469          

BLAST of EWM28618.1 vs. NCBI_GenBank
Match: gi|630351813|ref|XP_007865207.1| (amidase signature enzyme [Gloeophyllum trabeum ATCC 11539] >gi|521726281|gb|EPQ56481.1| amidase signature enzyme [Gloeophyllum trabeum ATCC 11539])

HSP 1 Score: 236.884 bits (603), Expect = 1.752e-66
Identity = 191/633 (30.17%), Postives = 307/633 (48.50%), Query Frame = 0

Query:    1 MFWWLSTAAERLQRKARAKRAEFEAKWAQPLPPLAPELDAAKETKIALELSIEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGESDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFRPASKDALVVELLRDAGAIVLCKTNVPQCLMLP-ETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIVMPSKSGKMGMQAIRATAGPLANRVEDVALVCQAWWVNKMWARDPY--VVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEGGLRSYIDALEGEAMIPMYRNIRLAAC---FPDFLRPL-LHTALYLTGEKRRRFLVKNMRRDGLATRTYWQYCEELQNYQ-----EAWTRMMQTQKLDGFLCPSVALPAFPHGWSSHLESSCSYNLFLNLLHYPAGVIPVTQVRADEAVYPISDLPRMQRDSIARAAAI---------------AMQGSEALPVSVQVAFPPFQDEMCLHVMKDLQDAVG 606
            M W  ++ +   +  A++KRAE E   A      A E  A+   +I  + +  ++V  ++ G +++  V+     RA         N +T+VLFE+A  EA+  D +  +        G L G+P S KDH ++ G D+T+G +    +P+ KDA +V L R  GAI   KTNVPQ + L  E SN +WG T +PW+   + GGSSGGEAAL+A+ G+ +G G+D+GGS R PA +SG    +PT  RVS +G+    +  + G + I+ TAGP+A  VED+ ++ +      +   D Y  V P P+                                 ++A  P  +++R G++  D F +   A KRAV E  +AL+  G +E VE ++   T     + ++  I T++G     ++A  G    P   N+ LA      P  +R + L    Y+ G+     +V   R    + + YW++  +  +Y+     EAWT+    Q+ DG + P  A+P+ PHG  +HL    +  +  N+L+YPAGV+PVT+V       P  D    +   +  A A+               AM+G   +PV VQV   P++DE  + +M+ +  A+G
Sbjct:    1 MIWPFNSNS--FEVIAKSKRAERERALAS-----AQEYSASTHGQIT-KATASEIVKRIEHGEWTASQVLEAFIARATL--AEKTTNCVTEVLFEQARREAQELDDEFSK---TKRLKGPLHGVPASFKDHFEIAGVDATSGYSHWANQPSEKDAAMVTLYRSMGAIPFVKTNVPQNMALSFECSNPLWGRTLNPWSAKYTCGGSSGGEAALLAMDGAGIGAGSDVGGSLRIPASYSGIYSLRPTAGRVSMEGV----RDLEPGFEGIKTTAGPMARCVEDLEIISRG----ILGISDDYNEVAPTPW---------------------------------KDAELP--KRLRFGYYTDDGFVKASPANKRAVLETVKALQEQG-HECVEFKIPDPT---RPLEVFVAITTSDG--YKTLEAHTGPD--PKESNLFLATLGPKLPGLIRSIGLWLMKYVIGDHVSIPIVAASRAK--SVKEYWEWTAKRDDYRRLFYDEAWTK----QQFDGIIAPVQAMPSIPHGGCAHLSPLAAATILYNVLNYPAGVVPVTRVD------PAKDQLTQEWQHLHGAKAVESMIYKGCKPTYNPEAMRG---IPVGVQVVGKPWEDEKVIGMMRVVDVALG 554          

BLAST of EWM28618.1 vs. NCBI_GenBank
Match: gi|829100400|ref|XP_001007347.2| (amidase [Tetrahymena thermophila SB210] >gi|586745740|gb|EAR87102.2| amidase (macronuclear) [Tetrahymena thermophila SB210])

HSP 1 Score: 229.95 bits (585), Expect = 1.674e-63
Identity = 181/602 (30.07%), Postives = 283/602 (47.01%), Query Frame = 0

Query:   14 RKARAKRAEFEAKWAQPLPPLAPELDAAKETKIALELSIEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGESDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFRPASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIVMPSKSGKMGMQAIRATAGPLANRVEDVALVC------QAWWVNKMWARDPYVVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEGGLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTALYLTGEKRRRFLVKNMRRDGLATRTYWQYCEELQNYQEAWTRMMQTQKLDGFLCPSVALPAFPHGWSSHLESS--CSYNLFLNLLHYPAGVIPVTQVRADEAVYPISDLPRMQRDSIARAAAIAMQGSEALPVSVQVAFPPFQDEMCLHVMKDLQDAVGF 607
            RK   KR +       P P L+ ++         L   I  L  ++++    S D+V++ ++R ++ G   G+  +T + +EEA   A+  D+ R+           L GIP+S+K+  D KG  +T G+  R      +D   V+LL+  GAI   +TNVPQ  M+ E+ N+++G   +PW+ T+ AGGSSGGE A VA R S  G+G+DIGGS R PA   G  GFKPT  R    G    SK+   G + +   +GP+   V+D+ L        Q     K+  RDP+    P          T++  + E     +Q                    R G+F +   F+   A +RAV  A + L + G ++LVE+ L       ++++  +         +   D L  E  +  Y  I + A  P  ++  L    Y+ GEK    L+    R  +      QY  ++   Q+ + R+    +++  +CPS   PA PH  SS L+S+  C Y    N L++P GV+PVT+V+ +E  +  S   R++     R      Q +E LPV+VQV  PPF++E CL+VMK L D + F
Sbjct:   52 RKFIQKRNQDLKNLIIPTPNLSQDI-----INKVLNSDITQLKKMLEEKIVKSVDLVNIFSQRVQKHGIEFGI--VTHLKYEEAIEAAKECDKLRKENSPLCSLP--LFGIPISMKETFDEKGYPATIGSIFRLDHIPKEDGFCVKLLKSGGAIPFLRTNVPQAAMIYESVNDVYGRVLNPWDKTKYAGGSSGGEGAAVAARMSPGGMGSDIGGSIRIPAAMCGVYGFKPTAQRTIMSGHTFYSKAFN-GQKTVLCASGPICKSVDDLILFFRQLSDPQYLQKFKLHERDPF---FP----------TQQINETELSNNKKQR-------------------RFGYFKTLKSFDCTLANQRAVEIAVEKLRAQG-HQLVEVDLPDV----DSIKNGFIQFLLSDSFQGIYDLLSQEDTVKEYLMIDIFASTPPLIKRFLSVLFYMLGEKSFAELLPQTNRLQVEELNKLQY--KIGQLQQDYLRLFDDHEIEAIICPSFGAPALPH--SSSLDSTPVCLYTFIWNYLNFPCGVLPVTKVQKEEQHFNNS---RIKELPAKRVDFYMKQNTEGLPVNVQVVAPPFKEETCLNVMKILDDEIQF 599          

BLAST of EWM28618.1 vs. NCBI_GenBank
Match: gi|908390665|gb|KND91301.1| (Acetamidase, partial [Tolypocladium ophioglossoides CBS 100239])

HSP 1 Score: 228.024 bits (580), Expect = 6.407e-63
Identity = 185/567 (32.63%), Postives = 268/567 (47.27%), Query Frame = 0

Query:   52 IEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGESDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFRPASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVS--GKGIVMPSKSGKMGMQAIRATAGPLANRVEDVALVCQAWWVNKMWARDPYVVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDG-WEAVRLYY--NIMTAEGGLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTALYLTGEKRRRFLVKNMRRDGLATRT--YWQYCEELQNYQEAWTRMMQTQKLDGFLCPSVALPAFPHGWSSHLESSCSYNLFLNLLHYPAGVIPVTQVRADEAVYPISDLPRMQRDSIARAA-----AIAMQGSEALPVSVQVAFPPFQDEMCLHVMKDLQDAVG 606
            IE+LV  +Q  +  + D++    K A  F   A  N +T++L ++A  E   KD       G  +  G L GIP+S+KD IDVKG DST G  C+   P  +D   V LL+D GA+   KTN+P  L+  E++N+IWG +++P+N   + GGS+GGE AL+A+ G  +GIG+D+ GS R PAHFSG    K +  R    G    MP + G      + A   P+A  + D+    +A      W  D    P+P     +R  V KE ++                          + +R+G F +D   +P  A KRA+   + AL   G +++VE+   +  +G + A RL     + T EG LR+      G A +  Y      A  P F++ + +  +      RR  L   + RD   T T  YW      + Y+  W  M    KLD  + P  A PA PH       SSC+Y    NL+ Y AGVIPVT V   +   P S+    + + IAR A     A AM G   LPV VQV     ++E  L +M  ++DA+G
Sbjct:   77 IEELVQDVQKDSSKAADLLLTYGKVA--FKAHAKTNCVTEILIKDA--EKWIKD-------GSINFKGPLAGIPISLKDTIDVKGYDSTVGMTCKARHPRPEDGATVRLLKDLGAVPYVKTNIPITLLSFESANDIWGRSTNPYNSKYAPGGSTGGEGALLAL-GGRIGIGSDVAGSVRCPAHFSGIYALKCSTGRWPKLGMNTSMPGQDG------VPAVYSPMARTLNDLLYFTRAVIQKGTWNYDHSCHPMP-----WRSDVVKEYKE-------------------------KKTMRVGVFRTDGIIDPSPACKRALEITEAALRRAG-HQIVEIDPPSPYEGLYLASRLLCADGLGTIEGFLRTGEWNDRGAAQMSYY------AKLPRFIKYVHYLWVRYV---RRDPLWAGLLRDWHPTSTQEYWSLIARREAYKRGWYEMWDDAKLDFIIAPPNATPAVPHNGMHDAVSSCAYTFLFNLIDYSAGVIPVTHVDRIKDRLP-SNFNVRKLNGIARGAYKLYDAEAMHG---LPVGVQVIGRRLEEEKVLTLMGRVEDALG 581          

BLAST of EWM28618.1 vs. NCBI_GenBank
Match: gi|754351932|ref|XP_004343422.2| (hypothetical protein CAOG_07548 [Capsaspora owczarzaki ATCC 30864] >gi|765556767|gb|KJE97076.1| hypothetical protein CAOG_007548 [Capsaspora owczarzaki ATCC 30864])

HSP 1 Score: 229.18 bits (583), Expect = 3.337e-62
Identity = 127/294 (43.20%), Postives = 174/294 (59.18%), Query Frame = 0

Query:   19 KRAEFEAKWAQPLP-PLAPELDAAKETKIALELSIEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGESDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFRPASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIVMPSKSGKMGMQAIRATAGPLANRVEDVALVCQAWWVNKMWARDPYVVPLPFDHAAYR 311
            KRAE +A+   P    + PEL A     + + L + +LV  +Q   F+S  +V    +RA+  G     NA  +  F EA  EA+  D      G      G L GIP+SVKD  D KG DST G A +CF P+++D L+V L+R AG I   ++NVPQ LM+ ET N+IWG   +P+N +R AGGSSGGE  L+A   S +G+GTD+GGS R P+++ G  GFKPTP R+S  G+     S + G QAI   AGP+   VED+ ++ + WWV+ +W RDPYV  +PF+ A YR
Sbjct:   61 KRAERDARRLVPFECNVDPELQA-----LIISLPVHELVQGIQSHRFTSEQIVRTYCQRAKLCGERFNSNA--EEFFHEAIEEAKEADAYFASTG---QLKGPLHGIPISVKDQFDQKGADSTCGMAAKCFHPSAEDGLLVHLIRTAGGIPFVRSNVPQMLMVTETRNDIWGTALNPYNTSRVAGGSSGGEGVLLACAASPLGLGTDVGGSIRVPSNWCGIYGFKPTPQRMSTVGLTN-GMSPQYGQQAILPVAGPMGRCVEDLTIIMKCWWVDALWNRDPYVPRMPFNDAIYR 343          

HSP 2 Score: 137.117 bits (344), Expect = 3.318e-30
Identity = 93/263 (35.36%), Postives = 130/263 (49.43%), Query Frame = 0

Query:  347 RKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEGGLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTALYLTGEKRRRFLVKNMRRDGLATR-TYWQYCEELQNYQEAWTRMMQTQKLDGFLCPSVALPAFPHGWSSHL-ESSCSYNLFLNLLHYPAGVIPVTQVRADEAVYPISDLPRMQRDSIARAAAIAMQGSEALPVSVQVAFPPFQDEMCLHVMKDLQDAVGF 607
            RK+R+G+F +   FE   A  RAV+E   +L   G +ELV        DG   +     +++ + GL +YI  L+GEA+   Y  +   +      R  L  +    G  R   LV   R  G+     Y +Y  + + Y+ A+    Q   LD  +CP++ LPA PH  ++ L  + C  NLF NLLH PAG +PVT VRADE  Y       + RD+    A  A  G+  LPV VQV   PFQDE+CLHVM  ++   G 
Sbjct:  478 RKLRIGYFTTTALFESSPACARAVQEVVASLTEAG-HELVFFEPPNLQDGAMTL---MGLLSCDRGLEAYIRGLQGEALNDAYEPLYQGSIMSTAARTELADSAERQGMWRLAGLV---RATGVKNAFEYKEYIHKAKTYKNAFVHAWQAANLDALICPTMPLPALPHLTTARLIPAHCYANLF-NLLHVPAGSVPVTHVRADEQCY-----TSVHRDAFTDLAVEASAGTAGLPVGVQVVALPFQDELCLHVMGLIERTNGL 727          

BLAST of EWM28618.1 vs. NCBI_GenBank
Match: gi|1025650133|gb|OAA37689.1| (amidase protein [Metarhizium rileyi RCEF 4871])

HSP 1 Score: 224.942 bits (572), Expect = 4.528e-62
Identity = 185/606 (30.53%), Postives = 276/606 (45.54%), Query Frame = 0

Query:   11 RLQRKARAKRAEFEAKWAQPLPPLAPELDAAKETKIALELSIEDLVALMQDGTFSSHDVVSVLAKRAREFGGPAGVNALTDVLFEEAFVEARRKDRDRQRGGGESDAVGVLEGIPLSVKDHIDVKGTDSTTGAACRCFRPASKDALVVELLRDAGAIVLCKTNVPQCLMLPETSNNIWGVTSSPWNLTRSAGGSSGGEAALVAVRGSLVGIGTDIGGSCRGPAHFSGCVGFKPTPARVSGKGIV--MPSKSGKMGMQAIRATAGPLANRVEDVALVCQAWWVNKMWARDPYVVPLPFDHAAYRRGVTKEAQQWERGGGGEQTRDGGKGRQEEASSPGGRKIRLGWFLSDDFFEPCRAGKRAVREAKQALESTGKYELVEMRLSTATDGWEAVRLYYNIMTAEG--GLRSYIDALEGEAMIPMYRNIRLAACFPDFLRPLLHTALYLTGEKRRRFLVKNMRRDGLATRT--YWQYCEELQNYQEAWTRMMQTQKLDGFLCPSVALPAFPHGWSSHLESSCSYNLFLNLLHYPAGVIPVTQVRADE----AVYPISDLPRMQRDSIARAAAIAMQGSEALPVSVQVAFPPFQDEMCLHVMKDLQDAVG 606
            R ++  R +R      +  P  PL  ++         + + IE+LV  +     S+ DV+    K A         N +T+VL ++A  E   KD       G  +  G L GIP+S+KD IDVKG DS+ G  C   +P S+D + V+LL++ GA+   KTN+P  L+  E+SN++WG +++P+N   + GGS+GGE AL+A+ G  +GIG+D+ GS R PAHF G    K +  R    G+   MP + G      I A   P+A  ++D+    +A      +  D    P+P     +R  V KE +Q                          + +R+G   +D   +P  A KRA+   + AL   G +E+VE+      + +EA+ L   ++ A+G   +R +     GE        +R  A  P   RP  +         RR  L   + RD   T T  YW      + Y+  W    Q   LD  + P  A PA PH       SSC Y    NLL Y AGVIPVT V   +    A + I +L  + R +     A  M G   LPV VQV     ++E  L +M+ ++DA+G
Sbjct:   13 RFKQDERVRRIRALTSYHAPFSPLDRDI---------INMPIEELVQQVHYDPGSAIDVLHTYGKVA--LKAHEKTNCVTEVLIKDA--EKWIKD-------GLINLKGPLAGIPISLKDTIDVKGYDSSVGVTCNVHKPKSEDGVTVKLLKELGAVPYIKTNIPITLLSFESSNDLWGRSTNPYNSKYTPGGSTGGEGALLAM-GGRIGIGSDVAGSVRCPAHFCGIYSLKCSTGRWPKLGMTTSMPGQDG------IPAVYSPMARTLDDLFYFTRAVLEKGTYNYDYSCHPIP-----WREDVVKEYKQ-------------------------KKAMRVGVLRTDGVVDPSPACKRALEMTESALRQDG-HEIVEI---IPPEPYEALYLASRLLCADGLDTVRGFFRT--GEWNDRGVAQMRFYAKLP---RPFKYLYYLWVRYIRRDTLWAGLLRDWHRTTTTEYWALIGRREAYKRQWWEYWQASNLDFIIAPPNATPAVPHDGMHDAVSSCGYTFMFNLLDYSAGVIPVTHVDKTKDQLPANFNIRNLNGIARGAYKLYNANDMHG---LPVGVQVIGRRLEEEKVLALMERVEDALG 549