EWM28621.1, cds2058 (CDS) Nannochloropsis gaditana

BLAST of EWM28621.1 vs. NCBI_GenBank
Match: gi|585111039|gb|EWM28621.1| (tRNA methyltransferase complex GCD14 subunit [Nannochloropsis gaditana])

HSP 1 Score: 957.207 bits (2473), Expect = 0.000e+0
Identity = 466/466 (100.00%), Postives = 466/466 (100.00%), Query Frame = 0

Query:    1 MDFALVQEWEREESQRGTPSTKPDGRVVCTATKRRRPFQAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGRFIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRSISPHGHLHTFEFNEARAIDAQVDFERNRVSHLVTVMHGDICQDLSLSDEESTIVCTSAHMETESDGKDPLGEHNGLAPNGTAAASQPEAPPPLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQFELRRTCMPHLDFGDGDSVGVGEDLGGAAEKGNGVRGDDQNTAHARETGPKKSESEGMEAWRSKRKHREAEGGKDARPFAKARPVMRGHTAFLTFAVAGLHPPKSLGPGAVGPTVPAPWIPTSGPPAPAPDPVDELAAEA 466
            MDFALVQEWEREESQRGTPSTKPDGRVVCTATKRRRPFQAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGRFIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRSISPHGHLHTFEFNEARAIDAQVDFERNRVSHLVTVMHGDICQDLSLSDEESTIVCTSAHMETESDGKDPLGEHNGLAPNGTAAASQPEAPPPLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQFELRRTCMPHLDFGDGDSVGVGEDLGGAAEKGNGVRGDDQNTAHARETGPKKSESEGMEAWRSKRKHREAEGGKDARPFAKARPVMRGHTAFLTFAVAGLHPPKSLGPGAVGPTVPAPWIPTSGPPAPAPDPVDELAAEA
Sbjct:    1 MDFALVQEWEREESQRGTPSTKPDGRVVCTATKRRRPFQAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGRFIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRSISPHGHLHTFEFNEARAIDAQVDFERNRVSHLVTVMHGDICQDLSLSDEESTIVCTSAHMETESDGKDPLGEHNGLAPNGTAAASQPEAPPPLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQFELRRTCMPHLDFGDGDSVGVGEDLGGAAEKGNGVRGDDQNTAHARETGPKKSESEGMEAWRSKRKHREAEGGKDARPFAKARPVMRGHTAFLTFAVAGLHPPKSLGPGAVGPTVPAPWIPTSGPPAPAPDPVDELAAEA 466          

BLAST of EWM28621.1 vs. NCBI_GenBank
Match: gi|553187502|ref|XP_005854625.1| (hypothetical protein NGA_2123900, partial [Nannochloropsis gaditana CCMP526] >gi|422294439|gb|EKU21739.1| hypothetical protein NGA_2123900, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 284.263 bits (726), Expect = 6.124e-92
Identity = 137/138 (99.28%), Postives = 137/138 (99.28%), Query Frame = 0

Query:   16 RGTPSTKPDGRVVCTATKRRRPFQAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGRFIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRS 153
            RGTPST PDGRVVCTATKRRRPFQAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGRFIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRS
Sbjct:    1 RGTPSTNPDGRVVCTATKRRRPFQAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGRFIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRS 138          

BLAST of EWM28621.1 vs. NCBI_GenBank
Match: gi|1210521292|dbj|GAX20040.1| (tRNA (adenine57-N1/adenine58-N1)-methyltransferase catalytic subunit [Fistulifera solaris])

HSP 1 Score: 270.781 bits (691), Expect = 9.382e-83
Identity = 169/405 (41.73%), Postives = 216/405 (53.33%), Query Frame = 0

Query:   34 RRRPFQAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGR-FIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRSISPHGHLHTFEFNEARAIDAQVDFERNRVSHLVTVMHGDICQDLSLSDEESTIVCTSAHMETESDGKDPLGEHNGLAPNGTAAASQPEAPPPLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHK-----QFELR---RTCMPHLDFGDGDSVGVGEDLGGAAEKGNGVRGDDQNTAHARETGPKKSESEGMEAWRSKRKHREAEGGKDARPFAKARPV--MRGHTAFLTFAVAGLH 427
            R+   Q GD+VV+ME  D  +  Y+E  GM+ NR GHF H D IGK  G+KV SR+  G  F  LL P+PELW  S+  RTQIV +LDQS ++F+L LRP M V+ESGTGSGAMSHAILR+I+PHG LHT+EFN+ RA  A+ +FE+N VSHLVTV H D+C  +                                           EA      PP              +DAVFLD+P PW A+P AA VLK NARIA+YSPC EQ  +T EA+++  FHS++TME R +     + E +   +   P  +  D ++  +       +  GNG + DD   +   ETG    E  G E           EG +       ARP   MRGH+AFLTFA AGLH
Sbjct:   85 RKETIQVGDLVVIMESFDRLTFAYIEEKGMYSNRHGHFSHKDFIGKPFGSKVRSRNNQGYGFCFLLKPTPELWARSLTHRTQIVHELDQSQIIFQLYLRPNMTVVESGTGSGAMSHAILRTIAPHGKLHTYEFNKMRADTARNEFEKNGVSHLVTVHHRDVCAKI-------------------------------------------EAEAGFDLPPC------------SVDAVFLDLPEPWHAVPHAAKVLKPNARIASYSPCVEQTQRTVEAMKKAGFHSMKTMEYRLQEHYVDEIEYKSPPKAKRPKFEAHDNEA-RIMSASAAGSASGNGEQSDD--LSDEVETG-DDGELAGDEM---------GEGNEIKAKMVVARPFASMRGHSAFLTFATAGLH 421          

BLAST of EWM28621.1 vs. NCBI_GenBank
Match: gi|397633665|gb|EJK71082.1| (hypothetical protein THAOC_07513 [Thalassiosira oceanica])

HSP 1 Score: 243.817 bits (621), Expect = 1.399e-71
Identity = 163/399 (40.85%), Postives = 211/399 (52.88%), Query Frame = 0

Query:   35 RRPFQAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGR-FIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRSISPHGHLHTFEFNEARAIDAQVDFERNRVSHLVTVMHGDICQDLSLSDEESTIVCTSAHMETESDGKDPLG----EHNGLAP-NGTAAASQPEAPPPLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQFELRRTCMPHLDFGDGDSVGVGEDLGGAAEK----GNGVRGDDQNTAHARETGPKKSESEGMEAWRSKRKHREAEGGKDARPFAKARPVMRGHTAFLTFAV 423
            R   + GD+VVV E  +  + VY +   +F NR GHF H+  IGK  G K+ S +  G  F+ LL P+PELW  S+  RTQIV +LD SM+V  L L P M V ESGTGSGAMSHAI+RSI+P G LHTFEFN+ RA  A+ +FE + + HLV V H D+C   SL  ++S      A  E ++ G    G    E+NG+   +GT      +A    AH                  A+FLD+P PWLA+P AA  L  N RI +YSPC EQ  +TC ALR + FHS+RT+E R K+F +    +P       D   V  +   + EK      GV   +Q    A   G K+   E       K+          ARPFA+    MRGH+AFLTFA 
Sbjct:  125 RELIKEGDLVVVFESFNDLNFVYAKKDAIFSNRNGHFKHNSFIGKPYGCKIRSNNNSGLGFLYLLRPTPELWARSLPHRTQIVHELDASMIVHYLNLCPNMTVCESGTGSGAMSHAIMRSIAPRGMLHTFEFNKVRADKAKKEFEEHGLGHLVQVHHRDVCGKKSLLLQKSGEKPGEAGDEPKAGGSRDDGPNESENNGVEEDDGTGGFLIGQAK---AH------------------AIFLDLPEPWLAVPHAAHTLCPNGRICSYSPCMEQTQRTCAALRRNGFHSLRTVEARLKEFYVASVELP---VPPTDLPVVLSEEASSIEKVRLANGGVLTPNQLEERADLLGQKRKGDEQESELEPKKV-------LCARPFAQ----MRGHSAFLTFAT 488          

BLAST of EWM28621.1 vs. NCBI_GenBank
Match: gi|1072231427|gb|OEU16159.1| (tRNA methyltransferase, partial [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 227.639 bits (579), Expect = 1.996e-68
Identity = 121/288 (42.01%), Postives = 161/288 (55.90%), Query Frame = 0

Query:   40 AGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSA-GGRFIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRSISPHGHLHTFEFNEARAIDAQVDFERNRVSHLVTVMHGDICQDLSLSDEESTIVCTSAHMETESDGKDPLGEHNGLAPNGTAAASQPEAPPPLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQ 326
             G++VV+ E  DS +  Y E G +F NR GHFHHDD IGK  G K+ SR+  G  F+ LL P+PELW+ S+  RTQI+  LD SM+VF L +RP M + ESGTGSGA+SH I+RSI+P G LHT+EFN+ RA  A+++F  N VSH+  V H D+C                             G+H      G     +P++                      +DA+ LD+P PW A+P AA  +K N+RI +YSPC EQ  KT  A++   FHSI+TME R K+
Sbjct:    3 VGNLVVIFENFDSSNFCYAEKGKIFNNRNGHFHHDDFIGKPYGCKIRSRNNRGWGFVYLLKPTPELWSQSLNHRTQIIHFLDASMIVFYLNIRPNMVICESGTGSGALSHCIMRSIAPKGMLHTYEFNQQRAETAKLEFANNGVSHISEVHHKDVC-----------------------------GKH------GPGGFDRPQSS---------------------VDAIVLDLPEPWSAVPYAAHCIKPNSRIVSYSPCVEQAQKTIIAMKTSGFHSIKTMEFRLKE 234          

BLAST of EWM28621.1 vs. NCBI_GenBank
Match: gi|223998472|ref|XP_002288909.1| (hypothetical protein THAPSDRAFT_261852, partial [Thalassiosira pseudonana CCMP1335] >gi|220976017|gb|EED94345.1| hypothetical protein THAPSDRAFT_261852, partial [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 228.409 bits (581), Expect = 6.991e-68
Identity = 149/388 (38.40%), Postives = 190/388 (48.97%), Query Frame = 0

Query:   39 QAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGR-FIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRSISPHGHLHTFEFNEARAIDAQVDFERNRVSHLVTVMHGDICQDLSLSDEESTIVCTSAHMETESDGKDPLGEHNGLAPNGTAAASQPEAPPPLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQ-FELRRTCMPHLDFGDGDSVGVGEDLGGAAEKGNGVRGDDQNTAHARETGPKKSESEGMEAWRSKRKHREAEGGKDARPFAKARPV--MRGHTAFLTFA 422
            Q  D+VV+ E  +  + VY  P  +F NR GHFHH+D IGK  G K+ SR+  G  F+ LL P+PELW  S+  RTQIV +LD +M+V  L + P M V ESGTGSGAMSH  LRS++P G LHT+EFN+ R   A+ +F  + + HLV V H D+C                        GK  L     L  NG + A                             A+FLD+P PWLA+P AA  +K N RI +YSPC EQ  +TC ALR+H FHSI+T+E R K+ FE                         + EK   V G +         GPKK + +       KR+ +    G        ARP   MRGHTAFLTFA
Sbjct:    2 QPTDLVVIFESFNDINFVYATPNDIFSNRNGHFHHNDFIGKPYGCKIRSRNNDGLGFLYLLRPTPELWARSLPHRTQIVHELDAAMIVHHLNVSPNMIVCESGTGSGAMSHVFLRSLAPMGKLHTYEFNKVRVEKARDEFVGHGLGHLVEVHHRDVC------------------------GKKAL-----LKLNGQSVA----------------------------HAIFLDLPEPWLAVPHAAHTIKPNGRICSYSPCMEQTQRTCTALRQHGFHSIQTVEARLKEYFET-----------------------ASLEKERHVNGGEV-------VGPKKEKEDTEPKQPKKRRKKGLSDGSSGNKILCARPFAQMRGHTAFLTFA 302          

BLAST of EWM28621.1 vs. NCBI_GenBank
Match: gi|553187499|ref|XP_005854624.1| (trna (adenine-n -)-methyltransferase catalytic, partial [Nannochloropsis gaditana CCMP526] >gi|422294438|gb|EKU21738.1| trna (adenine-n -)-methyltransferase catalytic, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 220.32 bits (560), Expect = 1.668e-67
Identity = 105/105 (100.00%), Postives = 105/105 (100.00%), Query Frame = 0

Query:  245 PLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQFELRRTCMPHLDFGDGDSVGVGE 349
            PLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQFELRRTCMPHLDFGDGDSVGVGE
Sbjct:    1 PLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQFELRRTCMPHLDFGDGDSVGVGE 105          

BLAST of EWM28621.1 vs. NCBI_GenBank
Match: gi|298714706|emb|CBJ27631.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 223.016 bits (567), Expect = 1.135e-63
Identity = 149/394 (37.82%), Postives = 196/394 (49.75%), Query Frame = 0

Query:   41 GDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGRFIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRSISPHGHLHTFEFNEARAIDAQVDFERNRVSHLVTVMHGDICQDLSLSDEESTIVCTSAHMETESDGKDPLGEHNGLAPNGTAAASQPEAPPPLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQFELRRTCMPHLDFGD-----GDSVGVGEDLGGAAEKGNGVRGDDQNTAHARETGPKKSESEGMEAWRSKRKHREAEGGKDARP-----FAKARPVMRGHTAFLTFAVA 424
            GDMVV  E  +      ++   ++Q R G FHHD+IIGK  G++V SR+  G ++  L P+PELW+  +  RTQIVQD DQS+V+F+L L+PG  V+ESGTGSG MS AI+R+I+P G LH+FEFNE RA  AQ +FE N +  LV  +H D              VC +             G+  G                                L G+ DAVFLD+P PWLA+  A   LK   ++ +YSPC EQV KTCEALR   FHS+ T+E R +              GD     G      E+ GG+     GV  DD     A  TG  ++E    +A     +    + G   +P      A+  P+MRGHTAFLTFA A
Sbjct:   30 GDMVVFFEGREPIGFDRMKAKTIWQCRHGAFHHDEIIGKPFGSRVFSRTTKG-YVYALSPTPELWSHVVPNRTQIVQDFDQSIVIFRLDLKPGDTVVESGTGSGVMSTAIMRTIAPSGFLHSFEFNEFRATRAQEEFEENALGGLVKAVHRD--------------VCAAR------------GDGGGFGEE----------------------------LDGKADAVFLDLPEPWLAVAHAKMTLKPGKKLCSYSPCIEQVMKTCEALRSEGFHSVSTIEFRLRNINYAEVEGIKPMEGDQAALPGSKKRSREEGGGSGAATPGV--DD----GASRTGRGRNERLQPKAAIRAAEAAAEKVGGARKPPLKLVCAQPFPLMRGHTAFLTFATA 362          

BLAST of EWM28621.1 vs. NCBI_GenBank
Match: gi|855308789|ref|XP_012896635.1| (uncharacterized protein [Blastocystis hominis] >gi|300122013|emb|CBK22587.2| unnamed protein product [Blastocystis hominis])

HSP 1 Score: 214.927 bits (546), Expect = 1.314e-61
Identity = 130/365 (35.62%), Postives = 185/365 (50.68%), Query Frame = 0

Query:   35 RRPFQAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGRFIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRSISPHGHLHTFEFNEARAIDAQVDFERNRVSHLVTVMHGDICQD-LSLSDEESTIVCTSAHMETESDGKDPLGEHNGLAPNGTAAASQPEAPPPLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQFELRRTCMPHLDFGDGDSVGVGEDLGGAAEKGNGVRGDDQNTAHARETGPKKSESEGMEAWRSKRKHREAEG 398
            +R    GD V + ER DS   V+L+ G  +Q  LG F HDD+IGK  G+ + S++ G +++ +L P+ E+W+ S+K RTQI+  LD S+++F L ++PG RV+ESGTGSGA+S  I R +SP GHL TFE+NE RA +A+ +FERN +S +++V H D   D   + DE +  +C  A                                    HP               +DAVFLD+P P+ AI  A  VLK N RI  +SPC EQV + C  LRE+ F  I TMEV  +Q+EL           D ++     +                + A A ET   K E EG  A  ++ +  E++G
Sbjct:    6 KRVAAEGDFVFLFERKDSIIPVFLKHGEKYQCILGAFLHDDMIGKEFGSIITSQT-GNKYLYMLRPTAEMWSGSMKTRTQIIFALDASVIIFNLFIKPGSRVVESGTGSGALSTDIARVLSPIGHLFTFEYNEMRATEARAEFERNGLSDIISVFHRDAYTDGFVVHDESNEKLCVDAE-----------------------------------HP---------------VDAVFLDLPKPYNAIKHACEVLKYNGRICCFSPCIEQVQENCRTLRENKFELIETMEVLVRQYELNSI--------DNNNSYTTRNRNRERNAAAAKAKGKGSEASAMETESNKEEGEGNNAEVNESEKMESKG 311          

BLAST of EWM28621.1 vs. NCBI_GenBank
Match: gi|1032149764|gb|OAO13673.1| (tRNA (adenine-N(1)-)-methyltransferase catalytic subunit [Blastocystis sp. ATCC 50177/Nand II])

HSP 1 Score: 203.371 bits (516), Expect = 5.829e-58
Identity = 129/390 (33.08%), Postives = 197/390 (50.51%), Query Frame = 0

Query:   35 RRPFQAGDMVVVMERHDSYSHVYLEPGGMFQNRLGHFHHDDIIGKHVGAKVMSRSAGGRFIRLLLPSPELWTMSIKQRTQIVQDLDQSMVVFKLGLRPGMRVLESGTGSGAMSHAILRSISPHGHLHTFEFNEARAIDAQVDFERNRVSHLVTVMHGDICQD-LSLSDEESTIVCTSAHMETESDGKDPLGEHNGLAPNGTAAASQPEAPPPLAHPPAVSVAPVARTLRGRIDAVFLDVPSPWLAIPLAAAVLKANARIATYSPCFEQVFKTCEALREHNFHSIRTMEVRHKQFELRRTCMPHLDFGDGDSVGVGEDLGGAAEKGNGVRGDDQNTAHARETG-PKKSESEGMEAWRSKRKHREAEGGKDARPFAKARPVMRGHTAFLTFA 422
            RR  + GD+V++ ER DS   V L+ G  +Q+  G F HD++IGK  G+ V++  AG +++ +L P+ ELW+ S++ RTQI+  LD S+++F L L+PG RV+ESGTGSGA+S  I R + P GHL TFE+NE R+ +A+ +F RN + ++++V H D   D   + DE+ +       +  ++D                             HP               +DAVFLD+P P+ AI  A  VLK N RI  +SPC EQV + C  LRE  F  I TME   +++EL       +D  + ++V     +    +KG  ++ +   T    +   P  S  E +                ++   +K   + R HTA+LTFA
Sbjct:    6 RRVAKEGDLVIIYERKDSIVPVILKSGDKYQSIFGAFLHDNMIGKEYGS-VITSQAGNKYVYILKPTAELWSGSMRTRTQIIFTLDASVIIFNLFLKPGCRVVESGTGSGALSTDIARVLGPMGHLFTFEYNELRSNEARNEFCRNGLGYIISVFHRDAYADGFVVEDEDES-------LRVDAD-----------------------------HP---------------VDAVFLDLPKPYAAIDNACKVLKYNGRICCFSPCIEQVQENCRVLREKGFELIETMESIIREYELNT-----IDNNNSNTV----RMRTLEKKGVSLKREVPTTEITNDLFVPSASVMETL----------------NSMVLSKGEHIGRAHTAYLTFA 318