EWM28552.1, cds2055 (CDS) Nannochloropsis gaditana

BLAST of EWM28552.1 vs. NCBI_GenBank
Match: gi|585110970|gb|EWM28552.1| (diphthamide biosynthesis [Nannochloropsis gaditana])

HSP 1 Score: 1113.21 bits (2878), Expect = 0.000e+0
Identity = 544/544 (100.00%), Postives = 544/544 (100.00%), Query Frame = 0

Query:    1 MAVLDNATDRSIEELSDLYECDRTLDAIERNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQSIATCLSTTLLARSKEKRAFLFYELAYEAIIPDVALEVNGQLVGSHVEVTTARLPLASVSPILPFSEEGQYLASQATGCEKEAGKVIFAGGLELRVENNGEEGEGEGGDAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGGKSWDGSYSTDFRELESLCQSGAQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYKGGVGGKAKGTLPLEGSTALTKFSSPAGDFLQLREYRGLGHDKHKNAVCETETGDRAEDREGGAKPPAGIVEGRRGIASDYDNV 544
            MAVLDNATDRSIEELSDLYECDRTLDAIERNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQSIATCLSTTLLARSKEKRAFLFYELAYEAIIPDVALEVNGQLVGSHVEVTTARLPLASVSPILPFSEEGQYLASQATGCEKEAGKVIFAGGLELRVENNGEEGEGEGGDAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGGKSWDGSYSTDFRELESLCQSGAQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYKGGVGGKAKGTLPLEGSTALTKFSSPAGDFLQLREYRGLGHDKHKNAVCETETGDRAEDREGGAKPPAGIVEGRRGIASDYDNV
Sbjct:    1 MAVLDNATDRSIEELSDLYECDRTLDAIERNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQSIATCLSTTLLARSKEKRAFLFYELAYEAIIPDVALEVNGQLVGSHVEVTTARLPLASVSPILPFSEEGQYLASQATGCEKEAGKVIFAGGLELRVENNGEEGEGEGGDAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGGKSWDGSYSTDFRELESLCQSGAQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYKGGVGGKAKGTLPLEGSTALTKFSSPAGDFLQLREYRGLGHDKHKNAVCETETGDRAEDREGGAKPPAGIVEGRRGIASDYDNV 544          

BLAST of EWM28552.1 vs. NCBI_GenBank
Match: gi|553187505|ref|XP_005854626.1| (diphthamide biosynthesis, partial [Nannochloropsis gaditana CCMP526] >gi|422294440|gb|EKU21740.1| diphthamide biosynthesis, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 451.055 bits (1159), Expect = 4.009e-155
Identity = 221/223 (99.10%), Postives = 221/223 (99.10%), Query Frame = 0

Query:  246 DAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGGKSWDGSYSTDFRELESLCQSGAQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYKGGVGGKA 468
            DAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGGKSWDGSYSTDFRELESLCQS AQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYKGGV GKA
Sbjct:    1 DAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGGKSWDGSYSTDFRELESLCQSCAQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYKGGVEGKA 223          

BLAST of EWM28552.1 vs. NCBI_GenBank
Match: gi|553187490|ref|XP_005854621.1| (diphthamide biosynthesis protein, partial [Nannochloropsis gaditana CCMP526] >gi|422294435|gb|EKU21735.1| diphthamide biosynthesis protein, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 416.387 bits (1069), Expect = 9.903e-142
Identity = 199/210 (94.76%), Postives = 205/210 (97.62%), Query Frame = 0

Query:    1 MAVLDNATDRSIEELSDLYECDRTLDAIERNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQSIATCLSTTLLARSKEKRAFLFYELAYEAIIPDVALEVNGQLVGSHVEVTTARLPLASVSPILPFSEEGQYLA 210
            MAVLDNATDRS+E+LSD YECDRTLD IE NGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQ+IATCLSTTLLARSKEKRAFLFYELAYEAIIPDVALE+N QLVGSHV+VT ARLPLASVSPI+PFSEEGQYLA
Sbjct:    1 MAVLDNATDRSMEKLSDQYECDRTLDTIECNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQAIATCLSTTLLARSKEKRAFLFYELAYEAIIPDVALEMNKQLVGSHVDVTAARLPLASVSPIMPFSEEGQYLA 210          

BLAST of EWM28552.1 vs. NCBI_GenBank
Match: gi|597932182|ref|XP_007320435.1| (hypothetical protein SERLADRAFT_472037 [Serpula lacrymans var. lacrymans S7.9] >gi|336369261|gb|EGN97603.1| hypothetical protein SERLA73DRAFT_184369 [Serpula lacrymans var. lacrymans S7.3] >gi|336382044|gb|EGO23195.1| hypothetical protein SERLADRAFT_472037 [Serpula lacrymans var. lacrymans S7.9])

HSP 1 Score: 304.679 bits (779), Expect = 1.116e-93
Identity = 210/555 (37.84%), Postives = 291/555 (52.43%), Query Frame = 0

Query:   12 IEELSDLYECDRTLDAIERNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPL------TQTLQSIATCLSTTLLARSKEKRAFLFYELAYEAIIPDVALEVNGQL--VGSHVEVTTARLPLASVSPILPFSEEGQYLASQATGCEKEAGKVIFAGGLELRVENNGEEGEGEGGDAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGGK-SWDGSYSTDFRELESLCQSGAQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYK-----GGVGGKAKGT---------LPLEGSTALTKFSSPAGDFLQLREYRGLGHDKHKNAVCETETGDRAEDREGGAKPPAGIVEGRRGIASDYDN 543
            I E  D YE +RT   I   G+ RVALQFPD +L D+  + + L        KR     H++        +V+ DTSYGSCCVDEVAAQH+DAD +VHYGHAC+S T RLPVLY+FGR+P+      TQ   ++ +  S +    S +K   L +++AY      +  +V  QL  +G  V V    LP         FS   Q  A   T  +     +   G L + +EN            V++YIG    +  L N+++   +CD   YDP+S        +R+ S +T++ L RRY +VQ+ARDA VFG+++GTLGV  Y+ +++ +R L+ARA KK+YT+ +GKLN AKLANF EIE FVLVACPE S++E   + F+ P++TP E+E+AL  + +W G Y  +F   E+L    A +++   T        AN E D  D P FSLV+G Y+     GG     +GT         L  +  T      S AG FLQ R YRGL          ET TG  A         P+ + +GR GIA  Y +
Sbjct:   42 ISEFEDFYEIERTARDIVDGGFKRVALQFPDLLLPDSVPIYRLL--------KRRVGEAHEL--------YVLADTSYGSCCVDEVAAQHVDADFMVHYGHACMSQTYRLPVLYIFGRRPIDVDDCATQLADTLKSSCSGSDPLESNKKPVILRHDVAYTYQADTIVAKVREQLEALGVSVPVLYQTLPSR-------FSPPAQKDADVTTSTQ-----LPIKGDLPVTLEN-----------GVILYIGG--ESLGLTNLLMTHASCDIYSYDPSSK------TVRQESTRTNRLLMRRYAIVQKARDADVFGILIGTLGVASYLPLISHIRTLLARARKKSYTISVGKLNPAKLANFMEIECFVLVACPENSVIE--AKEFYRPIITPYELEVALQAEGTWTGRYVLNF---ETLLSEHANEDQSFAT--------ANEEED-PDRPMFSLVTGKYRHAKRYGGHEETTEGTNEANPTAVILRNQDGTVSRLDDSAAGQFLQSRSYRGL----------ETRTGQDA---------PSILEQGRSGIARGYSD 516          

BLAST of EWM28552.1 vs. NCBI_GenBank
Match: gi|751714102|gb|KIM63122.1| (hypothetical protein SCLCIDRAFT_1214417 [Scleroderma citrinum Foug A])

HSP 1 Score: 296.204 bits (757), Expect = 2.982e-90
Identity = 200/544 (36.76%), Postives = 291/544 (53.49%), Query Frame = 0

Query:   17 DLYECDRTLDAIERNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQSIATCLSTTLLARSKEKRAFLF--YELAYEAIIPDVALEVNGQLVGSHVEVTTARLPLASVSPILPFSEEGQYLASQATGCEKEAGKVIFAGGLELRVENNGEEGEGEGGDAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGGK-SWDGSYSTDFREL--ESLCQSGAQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYKG----GVGGKAKGTLPLEGSTA---------LTKFS-SPAGDFLQLREYRGLGHDKHKNAVCETETGDRAEDREGGAKPPAGIVEGRRGIASDY 541
            D YE +RT   I+   + R+ALQFPDE+L D+  V + L           K+R +DV E      +V+ DTSYGSCCVDEVAAQH++ADL+VHYGHACLS TSRLPV+YVFG+KP+  T  S A  +   ++++     +F+   +++ Y     ++  +V  ++  +  ++    +P     P+L          SQ    +K++  V        +  N GE+       ++V+YIGE   +  L NI++  ++ +   Y+PA+  L      R  S +T++ L RR+  VQ+ARDA VFG++VGTLGV  Y+SV+  +R+LIAR+ KK+YT+ +GKLN AKLANF EIE FVL ACPE S++E   + F  P+VTP E+E+AL  + +W   Y  DF +L  E    + A           +    +  E++  D P FSLV+G Y+     GV G+       EG+TA         LTK S + A  FLQ R YRGL                   +   G   P+ + +GR GIA  Y
Sbjct:   39 DFYEIERTAKQIKDGAFKRIALQFPDELLHDSVPVYRLL-----------KQRMNDVQE-----LYVLADTSYGSCCVDEVAAQHVNADLIVHYGHACLSQTSRLPVIYVFGKKPIDVT--SCANQIGDLVVSKDSPWPSFVVIRHDVTYSHQAGEIVKQVQAKVALTGAQILCDDIP-TRADPLL----------SQPKSDDKDSRNVSVHESTYTKNPNGGED------HSIVVYIGE--ESLALTNILMTSSSNEIYSYNPATNGL------RLESGRTNKLLMRRFAAVQKARDADVFGILVGTLGVASYLSVITYLRQLIARSQKKSYTISVGKLNPAKLANFMEIECFVLAACPENSIIE--SKEFFKPIVTPYELEVALQPEGTWLDRYVLDFAQLIAEDRYTATATATATATATANETEESSGVENEDPDRPSFSLVTGKYRQARRFGVRGEQTCVDDSEGATAVALRNQESSLTKLSDNAAAMFLQSRTYRGL-------------------EARVGEDTPSVLEQGRSGIARGY 518          

BLAST of EWM28552.1 vs. NCBI_GenBank
Match: gi|1045914480|gb|OCH91570.1| (diphthamide biosynthesis protein [Obba rivulosa])

HSP 1 Score: 294.278 bits (752), Expect = 8.673e-90
Identity = 207/553 (37.43%), Postives = 295/553 (53.35%), Query Frame = 0

Query:   11 SIEELSDLYECDRTLDAIERNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQSIATCLSTTLLARS---KEKRAFLFYELAYEAIIPDVALEVNGQLVGSHVEVTTARLPLASVSPILPFSEEGQYLASQATGCEKEAGKVIFAGGLELRVENNGEEGEGEGGDAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMAL-GGKSWDGSYSTDFRELESLCQSGAQK-----ERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYK-----GGVGGKAKGTLP--------LEGSTALTKFSSPAGDFLQLREYRGLGHDKHKNAVCETETGDRAEDREGGAKPPAGIVEGRRGIASDY 541
            S EEL D Y+ +RT+  +E+  Y R+ALQFPDE+L+D+  V  F   ++++   RE               +V+ DTSYGSCCVDEVAAQH+DAD +VHYGHAC++ TSRLPV+YVFG+KP+    +  A  L  +L A S   + ++A L +++AY A   D  LE     + S   +  + +PL S             + S +T  + E  KV    G +   +   EEG       +++Y+G    +  L N+++  + C+   YDP +  +         S +T++ L RRY +VQ+ARDA VFG+++GTLGV  Y+ +++ +R+++ARA KK+YT+ +GKLN AKLANF EIE FVLVACPE SL++   + F  P+VTP E+E+AL   +SW G Y  +F +L +    G Q      E+G D DL              D+P FSL +G Y+     G     A    P         +G+ AL    S AG FLQ R YRGL          ET  G  A         PA + +GR GIA  Y
Sbjct:   31 SPEELEDYYDIERTVREVEKGDYKRIALQFPDELLRDS--VPIFRRLKARLSPGRE--------------LYVLADTSYGSCCVDEVAAQHVDADAMVHYGHACMTKTSRLPVIYVFGKKPVDP--EDCARKLLKSLEASSTVIENRKAILRHDVAY-AHRADKILEKLKAALPSSTTLQYSPIPLTSYP-----------VTSSSTSAQDEVLKV--TNGSDQTSDTVAEEGTPS---EIILYVGG--ESLSLTNLLMTHSHCEVHTYDPNTGTVCL------ASERTNKMLMRRYAVVQKARDADVFGILIGTLGVASYLPLISHLRKVLARAHKKSYTISVGKLNPAKLANFLEIECFVLVACPENSLID--AKDFLRPIVTPYELEIALRAPQSWTGRYVLEFEKLLAEAPDGTQNVSADPEKG-DEDL--------------DQPVFSLATGKYRHAKRYGAPETNANLNDPSSALVLRDQDGTVALK--DSAAGAFLQARTYRGL----------ETRIGQDA---------PAVLEQGRSGIARGY 502          

BLAST of EWM28552.1 vs. NCBI_GenBank
Match: gi|695383593|ref|XP_009517756.1| (hypothetical protein PHYSODRAFT_475147 [Phytophthora sojae] >gi|348690667|gb|EGZ30481.1| hypothetical protein PHYSODRAFT_475147 [Phytophthora sojae])

HSP 1 Score: 286.574 bits (732), Expect = 6.787e-87
Identity = 191/554 (34.48%), Postives = 282/554 (50.90%), Query Frame = 0

Query:   14 ELSDLYECDRTLDAIERNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQSIATCLSTTLLARSKEKRAFLFYELAYEAIIPDVALEVNGQLVGSHVEVTTARLPLASVSPILPFSEEGQYLASQATGCEKEAGKVIFAGGLELRVENNGEEGEGEGGDAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALG-GKSWDGSYSTDFRE----LESLCQSGAQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYKGG----------------VGGKAKGTLPLEGS---TALTKFSSPAGDFLQLREYRGLGHDKHKNAVCETETGDRAEDREGGAKPPAGIVEGRRGIASDYDN 543
            +L   Y+  RT+  I R GY ++ALQFPD +L DA +V Q L  +   + +R                FV+GDTSYGSCCVDEVAAQHL AD +VHYG  CLS T+++PV+Y FG  P    +      LS  + A    K   L YE  Y      V  E   +  G        +L L ++      +E  Q + + +TG EK +  V+  GG E+ V+++  E   E     ++YIG    +  L +I++R++A DC  Y+P           R+     +++L RR++LVQ+A++A ++G+++GTLGV +Y+ VV  +++LI ++G+K+Y  ++GK+N  KLAN+AEI+ FVLVAC + +L++   + ++ P+VTP E+++AL   + WDG Y TDF E    LE   QS  Q                + E D  D+P+FSLVSG YK                    G+A  +  L+     T LT + S A D+L  REY+GL                   D   G  P    VEG  GIA  Y +
Sbjct:   17 DLRVYYDIARTVAQIRRGGYKKIALQFPDSLLPDASQVQQELKDELVGQWER---------------VFVLGDTSYGSCCVDEVAAQHLVADCIVHYGRTCLSATTKIPVIYAFGNAP--TEVDDCVQQLSERIAAVDPIKSVVLLYEPRYHHASSAV-FEALKEKFGER------KLVLGTMKTFFDPTEPVQEVEA-STGDEKPSTSVLRIGGQEIWVDSDNAEVTPET--FALLYIGA--ESAHLTSILMRYSAVDCFSYNPEMM------STRKEGATVNRSLMRRFFLVQQAKEAQIYGILMGTLGVNKYLDVVHGLQKLIKKSGRKSYLFVVGKVNVPKLANYAEIDAFVLVACQQNTLMD--SKEYYKPIVTPYELQLALSPDEEWDGQYKTDFGEVIPALEKTAQSVEQA-------------AGDSEGDDADKPFFSLVSGTYKTSSRTTAVDREATTYALTASGEAAASTALQVKNERTELTTYHSEAADYLATREYQGL-------------------DPRIGKTPAHAAVEGSTGIARGYTH 501          

BLAST of EWM28552.1 vs. NCBI_GenBank
Match: gi|648159093|gb|KDR72825.1| (hypothetical protein GALMADRAFT_73379 [Galerina marginata CBS 339.88])

HSP 1 Score: 287.73 bits (735), Expect = 7.429e-87
Identity = 206/564 (36.52%), Postives = 297/564 (52.66%), Query Frame = 0

Query:    3 VLDNATD-RSIEELSDLYECDRTLDAIERNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQSIATCLSTTLLARSKEKRAFLFYELAYEAII--PDVALEVNGQLVGSHVEVTTARLPLASVSPILPFSEEG----QYLASQATGCEKEAGKVIFAGGLELRVENNGEEGEGEGGDAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSV-KTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGG-KSWDGSYSTDFRELESLCQSGAQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYK-----GGVGGKAKGTLPLEGSTALT---------KFSSPAGDFLQLREYRGLGHDKHKNAVCETETGDRAEDREGGAKPPAGIVEGRRGIASDYDN 543
            V D +TD  S +E  + YE +RT++ I    Y R+ALQFPDE+L  +  + + L  + +I     ++ Q    E      +++ DTSYGSCCVDEVAAQH++ADLVVHYGHAC++ TSRLPV+Y+FGRK +   ++  AT    T   R   +   L  E A +A++   DVA       +       + R  L+  +P +P   +      Y +SQ+T           A G E     N E  +       + Y+G    +  L N+++   +C+   YDP++  +     + + S  +T++ L RRY  VQ+ARDA VFG++VGTLGV  Y+ +++ +RRL+ R  KK+YT+ +GKLN AKLANF EIE FVLVACPE SL+ED  + F  P+VTP E+E+A+   ++W G Y  DF +L +   +          D  K+   A  ESD  D+P FSLV+G Y+     GG          +EG TA+          K SS AG FLQ R Y+GL          E   G+ A         P+ + +GR GIA  Y +
Sbjct:   25 VPDTSTDVLSPQEFDEFYEIERTVEEIVVGNYQRIALQFPDELLHVSVPIYRRL--KRRITDSVSEKGQSSTQE-----LYILADTSYGSCCVDEVAAQHVNADLVVHYGHACMTQTSRLPVVYIFGRKDIN--VEKCATGF-ITAFERDNLEEPQLEGERAKKAVLLRHDVAFSHKADSI-----FQSLREALSKCTPAIPVLYDKISTMTYPSSQSTSP---------AHGKEDLPRQNAE-NDIALEHTTIFYVGS--ESLGLTNLLMTNASCNVYSYDPSTEKV-----IHQSSTGRTNKLLMRRYVAVQKARDADVFGILVGTLGVASYLPLISYIRRLLRRKQKKSYTISVGKLNPAKLANFLEIECFVLVACPENSLIED--KEFLRPIVTPYELEVAMQAEQTWTGQYVLDFEKLLADATAAGSG------DEVKDAGTAAEESD-PDQPVFSLVTGTYRHAKRYGGESDTTSEQQVVEGVTAMVLRNQDDQVAKMSSAAGQFLQQRTYQGL----------EMRLGEDA---------PSVLEQGRAGIARGYQD 528          

BLAST of EWM28552.1 vs. NCBI_GenBank
Match: gi|1023221427|gb|KZT18344.1| (diphthamide biosynthesis protein [Neolentinus lepideus HHB14362 ss-1])

HSP 1 Score: 286.96 bits (733), Expect = 1.119e-86
Identity = 203/569 (35.68%), Postives = 292/569 (51.32%), Query Frame = 0

Query:   10 RSIEELSDLYECDRTLDAIERNGYCRV---------------ALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQSIATCLSTTLLARSKE--KRAFLFYELAYEAIIPDVALEVNGQLVGSHVEVTTARLPLASVSPILPFSEEGQYLASQATGCEKEAGKVIFAGGLELRVENNGEEGEGEGGDAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGG-KSWDGSYSTDFRELESLCQSGAQKERGLDTDLRKNGNCANCESDGE---DEPYFSLVSGGYKG--------------GVGGKAKGTLPLEGSTALTKFSSPAGDFLQLREYRGLGHDKHKNAVCETETGDRAEDREGGAKPPAGIVEGRRGIASDYDN 543
            RS+EE  + YE  R    I +  + RV               ALQFPDE+L D+  + ++L        KRE E+ H+  +   P  +V+ DTSYGSCC DEVAAQH+DADL+VHYGHAC+S T R+PV+YVFG+K L   ++  A+ L   + + + E  K   L +++AY  +   V   +   +  + V+     L L   SP  P        ++ A+  E    +     G     +++ +E   +   ++++YIG    N  L N+++  ++ +   YDP +         R  S +T++ L RRY  VQRARDA VFG++VGTLG+  Y+ +++ +R L+AR  KK YT+ +GKLN AKLANF EIE FVLVACPE SL+E   + FH  +VTP E+E+AL   ++W G Y  DF   E L    A++    D         AN ES  E   D+P FSL++G Y+               G GG +   L  +  T  T   S A +FLQ R +RGL          ET  G+ A         P+ + +GR GIA  Y N
Sbjct:   30 RSVEEFKNYYEIPRVATEIIQGSFKRVQHDQNETIFIDPLQIALQFPDELLHDSVPIYRYL--------KRELEKAHN--DDGPPELYVLADTSYGSCCADEVAAQHVDADLIVHYGHACMSKTYRMPVIYVFGKKHLD--VEDCASKLGEIVKSYTMEVEKIVVLRHDVAYAHLADYVVTRLRESIPDTVVQYNRPPLRL-DPSPPQP--------SAVASATESATAETQLNSG-----KHDSKEANRDVDPSLILYIGG--ENLGLTNLLMTHSSSEVYSYDPETR------STRLESGRTNKLLMRRYATVQRARDADVFGILVGTLGIASYLPLISHLRSLLARHRKKTYTISVGKLNPAKLANFMEIECFVLVACPENSLIE--SKEFHKAIVTPFELEIALQAEQTWTGRYVLDF---ERLLADHAREAPEDD---------ANRESTSEKDPDQPQFSLITGKYRTSRRFGGADANTSSVGEGGPSAVILRNQDDTVATLADSAAAEFLQSRAFRGL----------ETRVGEDA---------PSVLEQGRSGIARGYKN 531          

BLAST of EWM28552.1 vs. NCBI_GenBank
Match: gi|813135671|ref|XP_012198568.1| (diphthamide biosynthesis protein 2 [Saprolegnia parasitica CBS 223.65] >gi|641537002|gb|KDO30873.1| diphthamide biosynthesis protein 2 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 278.87 bits (712), Expect = 5.450e-84
Identity = 191/550 (34.73%), Postives = 272/550 (49.45%), Query Frame = 0

Query:   11 SIEELSDLYECDRTLDAIERNGYCRVALQFPDEMLKDAPRVCQFLMAQSKIRQKREKERQHDVSEHTEPLFFVIGDTSYGSCCVDEVAAQHLDADLVVHYGHACLSPTSRLPVLYVFGRKPLTQTLQSIATCLSTTLLARSKEKRAFLFYELAYEAIIPDVALEVNGQLVGSHVEVTTARLPLASVSPILPFSEEGQYLASQATGCEKEAGKVIFAGGLELRVENNGEEGEGEGGDAVVIYIGESENNKQLVNIMLRFNACDCLVYDPASPPLPAPPRLREVSVKTSQALKRRYYLVQRARDASVFGVVVGTLGVGRYMSVVAAVRRLIARAGKKAYTLLIGKLNAAKLANFAEIETFVLVACPEASLLEDGGRAFHAPVVTPLEMEMALGGKS-WDGSYSTDFRE-LESLCQSGAQKERGLDTDLRKNGNCANCESDGEDEPYFSLVSGGYKGGVGGKAK---------------GTLPLEGSTALTKFSSPAGDFLQLREYRGLGHDKHKNAVCETETGDRAEDREGGAKPPAGIVEGRRGIASDYDN 543
            S  +++  Y+   T   I  NGY  +ALQFPD +L DA +V   L         ++  R H++S       FV+GDTSYGSCCVDEVAAQHL AD+VVHYG ACLSPTS LPV+YVFG  P    +  +   L+ TL +  +  +  L YE  Y  I    A  +   L   H   +        V  ++       Y+    T  ++ A      GG+ + +       E       ++YIG+      L NI+LR     C  Y+P +       RL    +  ++ L RRY+LVQRA++A + G+++GTLGV +Y+ VV  ++ LIA++G+K+YT ++GK+N  KL+NFAEI+ FVLVACPE SL +     +  P++TP E+++AL   + W   Y +DF E L +L  + A  E                ++   DEPYFSLV+G Y     G A                  L  + +  L+ + S A +FL+ REYRGL                  E R G   P A  V+G  GIA  YD+
Sbjct:   29 SSADVAAFYDVRATAATIAANGYRAIALQFPDALLPDAQKVQSLL---------KDALRGHELSR-----IFVLGDTSYGSCCVDEVAAQHLKADVVVHYGRACLSPTSTLPVIYVFGNAPCE--VDQVCDGLNATLCSLEEPTQVVLMYEPCYAYI----AASMTSALAARHAATS-------FVCSVM----RTMYIPGATTTDDEAAKTTTTIGGMTVPMAAT----ELFSASTTLLYIGK--ETSHLTNILLRCGDTPCYSYNPTT----QLARLEGAGI--NRTLNRRYFLVQRAKEAQIIGILMGTLGVAKYLDVVHTMQDLIAKSGRKSYTFVVGKINVPKLSNFAEIDLFVLVACPENSLFDSTD--YFKPIITPYELQLALDDDAEWTAQYKSDFEEVLPALEATAATIE----------------DAPVSDEPYFSLVTGTYMSSRHGAASDDDDENEDAENDAATTALVAKDNHQLSVYKSEAAEFLKTREYRGL------------------EPRLGETAPHAA-VQGAIGIARGYDH 498