EWM28783.1, cds1949 (CDS) Nannochloropsis gaditana

Overview
NameEWM28783.1
Unique Namecds1949
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002458.1:566218..567282 -

Link to JBrowse

Properties
Property NameValue
Protein idEWM28783.1
Productputative trna pseudouridine synthase 1
Orig transcript idgnl|cribi|Naga_100002g63.4902.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002458.1supercontigCM002458.1:566218..567282 -
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Biological Process
TermDefinition
GO:0006139nucleobase-containing compound metabolic process
GO:0006396RNA processing
GO:0009451RNA modification
GO:0001522pseudouridine synthesis
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0003723RNA binding
GO:0009982pseudouridine synthase activity
Vocabulary: INTERPRO
TermDefinition
IPR020103PsdUridine_synth_cat_dom
IPR002501PsdUridine_synth
IPR014780tRNA_psdUridine_synth_TruB
Homology
BLAST of EWM28783.1 vs. NCBI_GenBank
Match: gi|585111206|gb|EWM28783.1| (putative trna pseudouridine synthase 1 [Nannochloropsis gaditana])

HSP 1 Score: 736.873 bits (1901), Expect = 0.000e+0
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0
Query:    1 MMRWKHCYHLKFCVLLLNIVTQSKADYRMYSGRKALVKPTFRLPKMCSFCPANLKVPNFRSLSPTRSCKERKYLARYERMCTVRGATPGTTAELPPLNMNGLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGVKVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEPRAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGPLSLDDCLPEEVWKNDAEAVVQHLINLRDDMYAASSDCPQ 354
            MMRWKHCYHLKFCVLLLNIVTQSKADYRMYSGRKALVKPTFRLPKMCSFCPANLKVPNFRSLSPTRSCKERKYLARYERMCTVRGATPGTTAELPPLNMNGLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGVKVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEPRAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGPLSLDDCLPEEVWKNDAEAVVQHLINLRDDMYAASSDCPQ
Sbjct:    1 MMRWKHCYHLKFCVLLLNIVTQSKADYRMYSGRKALVKPTFRLPKMCSFCPANLKVPNFRSLSPTRSCKERKYLARYERMCTVRGATPGTTAELPPLNMNGLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGVKVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEPRAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGPLSLDDCLPEEVWKNDAEAVVQHLINLRDDMYAASSDCPQ 354          
BLAST of EWM28783.1 vs. NCBI_GenBank
Match: gi|553194039|ref|XP_005855880.1| (pseudouridine synthase 1-like protein, partial [Nannochloropsis gaditana CCMP526] >gi|422293173|gb|EKU20473.1| pseudouridine synthase 1-like protein, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 691.034 bits (1782), Expect = 0.000e+0
Identity = 331/336 (98.51%), Postives = 333/336 (99.11%), Query Frame = 0
Query:    1 MMRWKHCYHLKFCVLLLNIVTQSKADYRMYSGRKALVKPTFRLPKMCSFCPANLKVPNFRSLSPTRSCKERKYLARYERMCTVRGATPGTTAELPPLNMNGLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGVKVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEPRAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGPLSLDDCLPEEVWKNDAEAVVQ 336
            MMRWKHCYHLKFCVLLLNIVTQSKADYRMYSGRKAL+KPTFRLPKMCSFCPANLKV NFRSLSPT SCKERKYLARYERMCTVRGATPGTTAELPPLNMNGLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGVKVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEPRAVQVDRLDLIHAEDETGH+LEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGP SLDDCLPEEVWKNDAEAVVQ
Sbjct:    6 MMRWKHCYHLKFCVLLLNIVTQSKADYRMYSGRKALIKPTFRLPKMCSFCPANLKVSNFRSLSPTTSCKERKYLARYERMCTVRGATPGTTAELPPLNMNGLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGVKVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEPRAVQVDRLDLIHAEDETGHKLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGPFSLDDCLPEEVWKNDAEAVVQ 341          
BLAST of EWM28783.1 vs. NCBI_GenBank
Match: gi|676397043|ref|XP_009041799.1| (hypothetical protein AURANDRAFT_5154, partial [Aureococcus anophagefferens] >gi|323447554|gb|EGB03470.1| hypothetical protein AURANDRAFT_5154, partial [Aureococcus anophagefferens])

HSP 1 Score: 221.476 bits (563), Expect = 1.733e-67
Identity = 131/245 (53.47%), Postives = 165/245 (67.35%), Query Frame = 0
Query:  101 GLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGVKVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEPRAVQVDRLDLIHAEDETG-----HRLEWPYFGLAIECQGGTYVRSLISDVAR--SVNARAHMTALTRTKHGPLSLDDCLPEEVWKNDAEAVVQHL 338
            G+ A++KP  W+S +VV K+R +LE+  +     K KVKVGHGGTLDP+A GVLV+GVGRGC+++  YLKG+K YRAV  LG E DT+DA G + E     HVT D+LE AL +F G I Q PP+YSAL  +GKRLYELAR+G +VE E R V + RL L    D+ G       L  P+FGL +EC GGTY+R+LISD+AR  +V + AHM  L RTK GP  L DCL E  W  D +A+ QHL
Sbjct:    1 GVCAVYKPPGWSSANVVAKLRGVLERAIKIPGQKKFKVKVGHGGTLDPLARGVLVVGVGRGCREMQGYLKGAKRYRAVAELGKETDTLDAEGAVVEEAPFDHVTLDALEAALPAFTGDIRQTPPMYSALHHEGKRLYELAREGIEVERESRPVTISRLAL----DDRGVGAVAGPLGLPHFGLDVECSGGTYIRTLISDLARHEAVKSVAHMVELERTKQGPFELGDCLHEADW--DFDAICQHL 239          
BLAST of EWM28783.1 vs. NCBI_GenBank
Match: gi|1210519808|dbj|GAX21360.1| (tRNA pseudouridine55 synthase [Fistulifera solaris])

HSP 1 Score: 213.386 bits (542), Expect = 6.548e-64
Identity = 113/235 (48.09%), Postives = 153/235 (65.11%), Query Frame = 0
Query:  101 GLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGVKV-----KVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEPRAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGPLSLDDCLPEEVWKND 330
            GL A+ KP NWTS++VV  +R +LE++AR R G K+     ++K+GHGGTLDP+ATGVLVLGVG+G + L  YL GSKAYRA   LG E +T+D  G IT      H+ P  LE     F G I Q PP++SA+++ G+RLY+ AR+G   +T+   V   RL  IH E + G+ ++ P F + +EC GGTY+RSLI D+A  ++  A  T L RTK GP +L+DCL +E W  D
Sbjct:   29 GLFAVHKPLNWTSSNVVSYIRGILERDARTR-GAKLPRRRPRIKIGHGGTLDPLATGVLVLGVGKGTKQLESYLSGSKAYRAGAALGFETNTLDMEGNITRRATFDHIQPIDLERVRPHFVGTIEQIPPLFSAIKQNGQRLYQKARKG---QTDDDVVIASRLVTIH-ELQFGN-VDLPQFDIQVECGGGTYIRSLIRDIAYQLDTVATTTYLQRTKQGPFTLEDCLEKEDWNAD 257          
BLAST of EWM28783.1 vs. NCBI_GenBank
Match: gi|551571022|ref|XP_005771422.1| (hypothetical protein EMIHUDRAFT_44604, partial [Emiliania huxleyi CCMP1516] >gi|485622650|gb|EOD18993.1| hypothetical protein EMIHUDRAFT_44604, partial [Emiliania huxleyi CCMP1516])

HSP 1 Score: 204.527 bits (519), Expect = 3.702e-61
Identity = 108/219 (49.32%), Postives = 143/219 (65.30%), Query Frame = 0
Query:  101 GLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGV---KVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQG--EDVETEPRAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHG 314
            G+V  +KP NWTS DVV K R  LE + ++       + ++KVGHGGTLDP+A G+LVLG+G GC+ L  YLKG KAYRA   LG+E DT D++G IT +    HVT  +L +A     G I+QRPP+YSAL+R GK L+ELAR G  E+ + EPR V V  L++   + E+G       F L ++C GGTYVR+LI D+ R+V + AHMT L RT+ G
Sbjct:    1 GIVVAYKPTNWTSFDVVAKARGCLEADLKKAGHTFRRRSRLKVGHGGTLDPLAEGLLVLGIGAGCKGLEGYLKGGKAYRATARLGTETDTQDSSGAITRSEPAEHVTLAALRDAAAGLTGSILQRPPIYSALKRGGKPLHELARAGKIEEGDVEPRRVTVHSLEVSRFDAESGS------FELLVDCAGGTYVRTLIVDLGRAVGSAAHMTGLVRTRVG 213          
BLAST of EWM28783.1 vs. NCBI_GenBank
Match: gi|551597689|ref|XP_005780927.1| (tRNA pseudouridine synthase, partial [Emiliania huxleyi CCMP1516] >gi|485633627|gb|EOD28498.1| tRNA pseudouridine synthase, partial [Emiliania huxleyi CCMP1516])

HSP 1 Score: 204.142 bits (518), Expect = 4.307e-61
Identity = 108/219 (49.32%), Postives = 143/219 (65.30%), Query Frame = 0
Query:  101 GLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGV---KVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQG--EDVETEPRAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHG 314
            G+V  +KP NWTS DVV K R  LE + ++       + ++KVGHGGTLDP+A G+LVLG+G GC+ L  YLKG KAYRA   LG+E DT D++G IT +    HVT  +L +A     G I+QRPP+YSAL+R GK L+ELAR G  E+ + EPR V V  L++   + E+G       F L ++C GGTYVR+LI D+ R+V + AHMT L RT+ G
Sbjct:    1 GIVVAYKPTNWTSFDVVAKARGCLEADLKKAGHTFRRRSRLKVGHGGTLDPLAEGLLVLGIGTGCKGLEGYLKGGKAYRATARLGTETDTQDSSGAITRSEPAEHVTLAALRDAAAGLTGSILQRPPIYSALKRGGKPLHELARAGKIEEGDVEPRRVTVHSLEVSRFDAESGS------FELLVDCAGGTYVRTLIVDLGRAVGSAAHMTGLVRTRVG 213          
BLAST of EWM28783.1 vs. NCBI_GenBank
Match: gi|942144451|ref|XP_005995377.2| (PREDICTED: probable tRNA pseudouridine synthase 1 [Latimeria chalumnae])

HSP 1 Score: 201.445 bits (511), Expect = 7.340e-59
Identity = 117/253 (46.25%), Postives = 156/253 (61.66%), Query Frame = 0
Query:   98 NMNGLVAIFKPRNWTSNDVVQKVRSLLEKEA------RRRAGVKVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEP-RAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGPLSLDD-CLPEEVWKNDAEA-VVQHLINL 341
            +++GL AI+KP+  TS DV+ K++  L +EA      R+R   K  +KVGHGGTLD  A+GVLV+G+GRG + L   L GSK Y AVG LG   DT+DA GT+TE     H+T D LE  L  F G  MQ PP+YSAL++ G+RL  L ++GE VE +P R V V  L L           + P F L +EC GG YVRSL+SD+ + ++  AH+  LTRTK GP +LD+  L EE W  D  A  ++H ++L
Sbjct:   19 SLSGLFAIYKPKGPTSADVLNKLKEKLMEEADMKPLSRKRK--KQTLKVGHGGTLDSAASGVLVVGIGRGTKMLGSMLSGSKKYTAVGELGKATDTLDAIGTVTEEKPYGHITKDDLEGILWKFTGDTMQVPPLYSALKKDGQRLSCLVKKGEAVEAKPARPVTVYSLSL--------QEFKPPLFTLNVECGGGFYVRSLVSDIGKELSTCAHVKELTRTKQGPFTLDEHILHEEKWTVDEIAQAIEHSMHL 261          
BLAST of EWM28783.1 vs. NCBI_GenBank
Match: gi|1210515176|dbj|GAX25888.1| (tRNA pseudouridine55 synthase [Fistulifera solaris])

HSP 1 Score: 195.282 bits (495), Expect = 1.254e-56
Identity = 105/241 (43.57%), Postives = 143/241 (59.34%), Query Frame = 0
Query:   95 PPLNMNGLVAIFKPRNWTSNDVVQKVRSLLEKEARRRAGV----KVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGE-DVETEPRAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGPLSLDDCLPEEVWKND 330
            P     GL A+ KP +WTS+ VV  +R +LE++AR R         ++K+GHGGTLDP+A GVLVLGVG G + L  YL GSKAYRA G  G E +T+D  G IT      H+    LE     F G I Q PP++SA+++ G+RLY+ AR+G  DV+   R+V +  L  ++        +  P F + +EC GGTY+RSLI D+A  ++  A  T L R K GP +L+DCL +E W  D
Sbjct:   46 PVYRAEGLFAVDKPLHWTSSQVVSYLRGILERDARTRGAQLPRRSPRIKMGHGGTLDPLANGVLVLGVGHGTKQLQSYLSGSKAYRAGGEFGFETNTLDMEGNITRRAAYDHI--HDLEHVRPHFVGTIEQIPPLFSAIKQDGQRLYQKARKGHTDVDIPARSVTIYELQFLN--------VNLPKFDIEVECGGGTYIRSLIRDIAYQLDTVATTTYLQRIKQGPFTLEDCLQKEDWTAD 276          
BLAST of EWM28783.1 vs. NCBI_GenBank
Match: gi|558217262|ref|XP_006135021.1| (PREDICTED: probable tRNA pseudouridine synthase 1 isoform X1 [Pelodiscus sinensis])

HSP 1 Score: 193.356 bits (490), Expect = 7.774e-56
Identity = 108/253 (42.69%), Postives = 159/253 (62.85%), Query Frame = 0
Query:   97 LNMNGLVAIFKPRNWTSNDVVQKVRSLL------EKEARRRAGVKVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEP-RAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGPLSLDD-CLPEEVWKND--AEAVVQHLI 339
             +++GL A++KP+  TS  V+ +++  L      +K+AR+R   K  +K+GHGGTLD  ATGVLV+G+G+G + L+  L GSK Y A+G LG   DT+DATG +TE      +T + LE  L  F+G IMQ PP+YSAL+R G+RL  L ++GE VE +P R V V  L L           + P F L +EC GG YVRSL+SD+ + +++ A++  LTRTK GP +L++  L E+ W  D  A+++ Q ++
Sbjct:   22 FSLSGLFALYKPKGPTSAHVLNQLKEKLLAEVGIQKDARKRK--KQILKIGHGGTLDSAATGVLVVGIGKGTKMLSNVLAGSKKYTAIGELGKATDTLDATGKVTEEKPYDQITKEDLERVLQKFKGDIMQVPPLYSALKRDGQRLSSLMKKGEAVEAKPARPVTVYSLSL--------QNFQPPLFTLDVECGGGFYVRSLVSDIGKELSSCANVQELTRTKQGPFTLEEHALHEDKWTIDEIAQSLKQSML 264          
BLAST of EWM28783.1 vs. NCBI_GenBank
Match: gi|1025094302|ref|XP_016343662.1| (PREDICTED: probable tRNA pseudouridine synthase 1 [Sinocyclocheilus anshuiensis])

HSP 1 Score: 192.971 bits (489), Expect = 1.507e-55
Identity = 106/239 (44.35%), Postives = 147/239 (61.51%), Query Frame = 0
Query:   98 NMNGLVAIFKPRNWTSNDVVQKVRSLLEKEA-------RRRAGVKVKVKVGHGGTLDPMATGVLVLGVGRGCQDLNLYLKGSKAYRAVGLLGSEYDTMDATGTITETVDVSHVTPDSLEEALVSFRGCIMQRPPVYSALRRKGKRLYELARQGEDVETEP-RAVQVDRLDLIHAEDETGHRLEWPYFGLAIECQGGTYVRSLISDVARSVNARAHMTALTRTKHGPLSLDD-CLPEEVW 327
            + NG+ AI+K +  TS DV+  ++  L KEA       R+R   K  +K+GHGGTLD  A+GVLV+G+G G + L   L GSK YRAVG LG   DT+D TG + E     H+T ++ EE L  F G IMQ PP+YSAL++ GKR+  L +QG++VE +P R V V  L L             PYF + +EC GG YVRSL+ D+A+++++ AH+  LTRTK G  +L++  L E+ W
Sbjct:   27 SFNGIFAIYKKQGPTSADVLNTLKKALLKEAGVANPNPRKRN--KQPLKIGHGGTLDSNASGVLVVGIGEGTKMLTTMLAGSKKYRAVGELGKATDTLDVTGNVVEEKSYDHITKEAFEEKLKQFTGEIMQVPPLYSALKKDGKRMSVLLKQGQEVEAKPARPVTVYSLSL--------QDFSPPYFTIDVECGGGFYVRSLVDDLAKALSSCAHIKELTRTKQGQFTLEEHALKEDRW 255          
The following BLAST results are available for this feature:
BLAST of EWM28783.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585111206|gb|EWM28783.1|0.000e+0100.00putative trna pseudouridine synthase 1 [Nannochlor... [more]
gi|553194039|ref|XP_005855880.1|0.000e+098.51pseudouridine synthase 1-like protein, partial [Na... [more]
gi|676397043|ref|XP_009041799.1|1.733e-6753.47hypothetical protein AURANDRAFT_5154, partial [Aur... [more]
gi|1210519808|dbj|GAX21360.1|6.548e-6448.09tRNA pseudouridine55 synthase [Fistulifera solaris... [more]
gi|551571022|ref|XP_005771422.1|3.702e-6149.32hypothetical protein EMIHUDRAFT_44604, partial [Em... [more]
gi|551597689|ref|XP_005780927.1|4.307e-6149.32tRNA pseudouridine synthase, partial [Emiliania hu... [more]
gi|942144451|ref|XP_005995377.2|7.340e-5946.25PREDICTED: probable tRNA pseudouridine synthase 1 ... [more]
gi|1210515176|dbj|GAX25888.1|1.254e-5643.57tRNA pseudouridine55 synthase [Fistulifera solaris... [more]
gi|558217262|ref|XP_006135021.1|7.774e-5642.69PREDICTED: probable tRNA pseudouridine synthase 1 ... [more]
gi|1025094302|ref|XP_016343662.1|1.507e-5544.35PREDICTED: probable tRNA pseudouridine synthase 1 ... [more]
back to top
Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna1954rna1954Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications