EWM29233.1, cds1432 (CDS) Nannochloropsis gaditana

BLAST of EWM29233.1 vs. NCBI_GenBank
Match: gi|585111755|gb|EWM29233.1| (serine hydroxymethyltransferase [Nannochloropsis gaditana])

HSP 1 Score: 1082.01 bits (2797), Expect = 0.000e+0
Identity = 524/524 (100.00%), Postives = 524/524 (100.00%), Query Frame = 0

Query:    1 MERGGRRWQLAILGLMGWCWNSCAAAFAVPPCYWPPLSLSVAAAAELAAPSTQSQCRASPHGSRLAHAQKSSTVAASVLSGHEAPSILADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRRVSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDMAHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRKELAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMELQRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFPS 524
            MERGGRRWQLAILGLMGWCWNSCAAAFAVPPCYWPPLSLSVAAAAELAAPSTQSQCRASPHGSRLAHAQKSSTVAASVLSGHEAPSILADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRRVSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDMAHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRKELAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMELQRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFPS
Sbjct:    1 MERGGRRWQLAILGLMGWCWNSCAAAFAVPPCYWPPLSLSVAAAAELAAPSTQSQCRASPHGSRLAHAQKSSTVAASVLSGHEAPSILADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRRVSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDMAHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRKELAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMELQRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFPS 524          

BLAST of EWM29233.1 vs. NCBI_GenBank
Match: gi|922857763|gb|KOO25971.1| (serine hydroxymethyltransferase [Chrysochromulina sp. CCMP291])

HSP 1 Score: 603.979 bits (1556), Expect = 0.000e+0
Identity = 305/458 (66.59%), Postives = 356/458 (77.73%), Query Frame = 0

Query:   66 AHAQKSSTVAASVLSGHEAPSILADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRRVSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDMAHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRKELAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMELQRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFP 523
            A A  SST AA    GH   S L+ +DPEV  +I+ E  RQ  G+ELIASENF S AV EALGS +TNKYSEG PGARYYGGNEH+DA+ERLC+ RAL L+ LS + WGVNVQPYSGSPANFA YTALL+PHDRIMGLDLPSGGHLTHGYY+ K+++SATSIYFESLPY VD  TGLVDYD L+  A LFRP+LI+AG SAYPRDWDY R+R IAD  GAYL+ DMAHISGL +A  C+NPF ++DVVTSTTHKSLRGPR+G+IF+RK+    V+ AVFP+LQGGPHNHQI ALAVAL+EAAAP F  DYI  VK NA+ALA +L   G+ +VT GT+NHL+LWD+RP G+TGSK+EKL EAV IS+NKN + GD SA +PGGVRVGTPAMTTRGL E DF  VA  L    +L+L++QA     KL DF   ++    EVRALR+RV  FAS F  P
Sbjct:   23 ASAPSSSTPAA----GH---SSLSKQDPEVCSLIEKEAARQRDGLELIASENFVSPAVREALGSCLTNKYSEGLPGARYYGGNEHIDAIERLCQKRALDLYGLSPDEWGVNVQPYSGSPANFAAYTALLQPHDRIMGLDLPSGGHLTHGYYSPKKKISATSIYFESLPYEVDATTGLVDYDGLEARARLFRPKLIIAGASAYPRDWDYGRMRQIADEVGAYLLNDMAHISGLAAARECHNPFEHADVVTSTTHKSLRGPRAGVIFYRKKFEAAVNGAVFPALQGGPHNHQIGALAVALREAAAPEF-GDYIRSVKANAKALARKLVSHGYEIVTGGTDNHLVLWDLRPLGLTGSKLEKLCEAVHISLNKNAVRGDTSALTPGGVRVGTPAMTTRGLNEADFEVVADHLHAAAQLSLQLQA-LSGPKLADFVHEIEG-SAEVRALRERVTEFASKFPMP 470          

BLAST of EWM29233.1 vs. NCBI_GenBank
Match: gi|544211744|ref|XP_005536271.1| (serine hydroxymethyltransferase [Cyanidioschyzon merolae strain 10D] >gi|449016583|dbj|BAM79985.1| serine hydroxymethyltransferase [Cyanidioschyzon merolae strain 10D])

HSP 1 Score: 588.186 bits (1515), Expect = 0.000e+0
Identity = 285/443 (64.33%), Postives = 346/443 (78.10%), Query Frame = 0

Query:   92 DPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRRVSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDMAHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRK-----------ELAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMELQRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFP 523
            DPEV  I++ E+ RQ  G+ELIASENF S AV+EALGS  TNKYSEG PG RYYGGN ++D +ERLC+ RAL+ F+LS   WGVNVQPYSGSPANFAVYTALL PHDRIMGLDLPSGGHLTHG+Y  K+RVSATSIYFESLPYRV+  TG +DYD L+K A+LFRP+L++AGGSAY R+WDYAR R IAD  GAYL+ DMAHISGLV+ G   +PFPY+DVVT+TTHKSLRGPR+GMIF+R+           +L +++  AVFP+LQGGPHNHQIAALAV L+E   P+FR  Y  +++RNA+ALA  L + G+ +VT GTENHL+LWD+RP GITGSK+EKL E V I++NKN + GD SA +PGGVR+GTPA+TTRG  E DF  VA FLD  +R+AL++Q  +  KKL DF  AM   + EV+ALR+ V+ FAS F  P
Sbjct:   83 DPEVYQILEKEKERQRRGLELIASENFTSAAVMEALGSAFTNKYSEGYPGRRYYGGNMYIDEVERLCQERALAAFSLSPTDWGVNVQPYSGSPANFAVYTALLNPHDRIMGLDLPSGGHLTHGFYTAKKRVSATSIYFESLPYRVNETTGYIDYDALEKQAALFRPKLLIAGGSAYAREWDYARFRQIADQSGAYLLVDMAHISGLVATGEAQSPFPYADVVTTTTHKSLRGPRAGMIFYRRSALAEIAPRGADLEQRIQEAVFPALQGGPHNHQIAALAVQLREVQTPAFRA-YAKQIRRNAQALARRLVQHGYDLVTGGTENHLVLWDLRPIGITGSKMEKLCEYVSITLNKNAVPGDTSALNPGGVRIGTPALTTRGFREADFEKVADFLDSCVRIALRIQEQS-GKKLNDFV-AMLPDDPEVQALRESVERFASTFPMP 522          

BLAST of EWM29233.1 vs. NCBI_GenBank
Match: gi|470508817|ref|XP_004349634.1| (serine hydroxymethyltransferase [Acanthamoeba castellanii str. Neff] >gi|440801528|gb|ELR22546.1| serine hydroxymethyltransferase [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 584.334 bits (1505), Expect = 0.000e+0
Identity = 285/456 (62.50%), Postives = 352/456 (77.19%), Query Frame = 0

Query:   78 VLSGHEAPSILADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRRVSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDMAHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRK----------ELAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMELQRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFP 523
            +LS H     LAD DPEV  +I+ E+ RQ++GIELIASENF S+AVLEALGS MTNKYSEG PG RYYGGNE +D +E LC  RAL  F+LS E WGVNVQPYSGSPANFA YTALL PHDRIMGLDLPSGGHLTHGY  +KR++SATSIYFES+PY+V   TGL+DYD L++ A+LFRP++I+AG SAYPRDWDY RLR IA+  GAYL+ DMAHISG+V+A  CN+PF Y DVVT+TTHK+LRGPR+G+IFFR+          +  ++++ AVFP+LQGGPH + IAA+AVALKEAA P F+  YI +VK+NA+ LA  L   G+SVVT GT+NHL+LWD+RP  +TGSK+EKL E V ISVNKN ++GD SA SPGGVR+G PAMT+RGLTE DF+ VA  L  G ++A+ +Q     K L ++  A++  E EV+AL++ V+ FAS F  P
Sbjct:    1 MLSQHAGNRTLADADPEVQGLIRLEKKRQLNGIELIASENFTSRAVLEALGSCMTNKYSEGLPGRRYYGGNEVIDQVENLCIKRALEAFHLSPEQWGVNVQPYSGSPANFAAYTALLNPHDRIMGLDLPSGGHLTHGYQTDKRKISATSIYFESMPYQVSYQTGLIDYDRLEENAALFRPKMIIAGASAYPRDWDYKRLRQIANKHGAYLLCDMAHISGIVAAQECNSPFEYCDVVTTTTHKTLRGPRAGLIFFRRGKNEATGAAYDYEDRINNAVFPALQGGPHENTIAAVAVALKEAAEPEFKT-YIQQVKKNAKVLAETLVSKGYSVVTGGTDNHLVLWDVRPQEMTGSKLEKLFELVSISVNKNAVYGDASALSPGGVRLGAPAMTSRGLTEADFVRVAELLHKGAQIAIAIQ-NKTGKLLKNYLPALETSE-EVKALKEEVEAFASTFPMP 453          

BLAST of EWM29233.1 vs. NCBI_GenBank
Match: gi|551625436|ref|XP_005792856.1| (serine hydroxymethyltransferase [Emiliania huxleyi CCMP1516] >gi|485647201|gb|EOD40427.1| serine hydroxymethyltransferase [Emiliania huxleyi CCMP1516])

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 299/440 (67.95%), Postives = 351/440 (79.77%), Query Frame = 0

Query:   86 SILADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRRVSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDM--AHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRKELAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMELQRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFP 523
            S L  ED  V  +I+AE  RQ  G+ELIASENFAS AV EALGSVMTNKYSEG PGARYYGGNEHVDALERLC+ARAL LF L  + WGVNVQPYSGSPANFA +TALLEPHDRIMGLDLPSGGHLTHGYYNE+RRVSATSIYFESLPY V   TGL+DYD L++ A LFRP+L++AG SAYPRDWDYAR+R IAD+ GA LM DM  AHISGLV+A    +PF + DVVTSTTHKSLRGPR+G+IF+RK     V+FAVFP LQGGPHNHQIAALAVAL+EA  P+FR+ YI RVK NA+ALA  L   G+++VT GT+NHL+LWD+RP  +TGSK+EKL EAVGI++NKN + GD SA++PGGVR+GTPA+TTRG  E DF+ VA +L + + LAL +Q  + + KL DF   M+A E  V  LR+RV+ FA+ F  P
Sbjct:   45 SALEREDGTVWELIEAEGRRQRDGLELIASENFASAAVREALGSVMTNKYSEGLPGARYYGGNEHVDALERLCQARALELFGLDPDEWGVNVQPYSGSPANFAAFTALLEPHDRIMGLDLPSGGHLTHGYYNERRRVSATSIYFESLPYEVSAETGLIDYDGLEERARLFRPKLLIAGASAYPRDWDYARMRRIADSVGALLMNDMAHAHISGLVAAEQAADPFEHCDVVTSTTHKSLRGPRAGVIFYRKRFESAVNFAVFPCLQGGPHNHQIAALAVALREAQQPAFRE-YIRRVKANAKALAAALVARGYTIVTGGTDNHLVLWDLRPQRLTGSKLEKLAEAVGITLNKNAVRGDVSAATPGGVRIGTPALTTRGFGEADFVRVAEYLHEAVELALALQQRSGT-KLKDFVAEMEA-EPAVADLRERVRAFATEFPMP 481          

BLAST of EWM29233.1 vs. NCBI_GenBank
Match: gi|551576247|ref|XP_005773799.1| (hypothetical protein EMIHUDRAFT_424674 [Emiliania huxleyi CCMP1516] >gi|485625504|gb|EOD21370.1| hypothetical protein EMIHUDRAFT_424674 [Emiliania huxleyi CCMP1516])

HSP 1 Score: 581.637 bits (1498), Expect = 0.000e+0
Identity = 299/440 (67.95%), Postives = 351/440 (79.77%), Query Frame = 0

Query:   86 SILADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRRVSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDM--AHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRKELAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMELQRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFP 523
            S L  ED  V  +I+AE  RQ  G+ELIASENFAS AV EALGSVMTNKYSEG PGARYYGGNEHVDALERLC+ARAL LF L  + WGVNVQPYSGSPANFA +TALLEPHDRIMGLDLPSGGHLTHGYYNE+RRVSATSIYFESLPY V   TGL+DYD L++ A LFRP+L++AG SAYPRDWDYAR+R IAD+ GA LM DM  AHISGLV+A    +PF + DVVTSTTHKSLRGPR+G+IF+RK     V+FAVFP LQGGPHNHQIAALAVAL+EA  P+FR+ YI RVK NA+ALA  L   G+++VT GT+NHL+LWD+RP  +TGSK+EKL EAVGI++NKN + GD SA++PGGVR+GTPA+TTRG  E DF+ VA +L + + LAL +Q  + + KL DF   M+A E  V  LR+RV+ FA+ F  P
Sbjct:   45 SALEREDGAVWELIEAEGRRQRDGLELIASENFASAAVREALGSVMTNKYSEGLPGARYYGGNEHVDALERLCQARALELFGLDPDEWGVNVQPYSGSPANFAAFTALLEPHDRIMGLDLPSGGHLTHGYYNERRRVSATSIYFESLPYEVSAETGLIDYDGLEERARLFRPKLLIAGASAYPRDWDYARMRRIADSVGALLMNDMAHAHISGLVAAEQAADPFEHCDVVTSTTHKSLRGPRAGVIFYRKRFESAVNFAVFPCLQGGPHNHQIAALAVALREAQQPAFRE-YIRRVKANAKALAAALVARGYTIVTGGTDNHLVLWDLRPQRLTGSKLEKLAEAVGITLNKNAVRGDVSAATPGGVRIGTPALTTRGFGEADFVRVAEYLHEAVELALALQQRSGT-KLKDFVAEMEA-EPAVADLRERVRAFATEFPMP 481          

BLAST of EWM29233.1 vs. NCBI_GenBank
Match: gi|1189394613|gb|OSX81493.1| (hypothetical protein BU14_0014s0043 [Porphyra umbilicalis])

HSP 1 Score: 577.785 bits (1488), Expect = 0.000e+0
Identity = 276/446 (61.88%), Postives = 343/446 (76.91%), Query Frame = 0

Query:   88 LADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRRVSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDMAHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRK----------ELAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMELQRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFP 523
            LA+ DPE+  ++QAER RQ  GIELIASENF S+AVLE LGS +TNKYSEG PGAR+YGGNE +D +E LC++RAL+ F LS + WGVNVQPYSGSPANFA YT LL PHDRIMGLDLPSGGHLTHG+Y  K+RVSATSIYFESLPY+VD  TGL+DYD L+  A+ FRP++++AGGSAYPR+WDYAR R++AD  GAYLM DMAHISGLV+ G  ++PF Y+DVVTSTTHKSLRGPR+G+IF+RK           +AE ++ AVFPSLQGGPHNHQI ALAV L E A P F   Y  +V  NA A A  L   G+ +VT+GT+NHL+LWD+RP G++G+K++ + +A  I++NKN++HGD SA +PGGVR+G+PAMT+RGL E DF  +A FL  G+ +A +VQA    KKL DF  A++ +  +V ALRD V+ FA  F  P
Sbjct:   72 LAETDPEMYELLQAERTRQQRGIELIASENFTSRAVLETLGSCLTNKYSEGYPGARFYGGNEVIDKIESLCQSRALAAFGLSPDEWGVNVQPYSGSPANFAAYTGLLNPHDRIMGLDLPSGGHLTHGFYTAKKRVSATSIYFESLPYKVDSATGLIDYDALESNAAAFRPKVVIAGGSAYPREWDYARFRSVADQSGAYLMVDMAHISGLVATGVVDSPFQYADVVTSTTHKSLRGPRAGVIFYRKTPLAAAGEGVNMAEAINAAVFPSLQGGPHNHQIGALAVQLSEVATPGFVA-YSHQVVANARACAATLMGHGYKLVTDGTDNHLVLWDLRPAGVSGAKMQAVFDACSITLNKNSVHGDTSAMNPGGVRIGSPAMTSRGLVEADFEQIAHFLHRGVVIAQQVQA-ISGKKLKDFLPALEGN-ADVAALRDEVESFAEGFHMP 514          

BLAST of EWM29233.1 vs. NCBI_GenBank
Match: gi|676383543|ref|XP_009035050.1| (hypothetical protein AURANDRAFT_53011 [Aureococcus anophagefferens] >gi|323454364|gb|EGB10234.1| hypothetical protein AURANDRAFT_53011 [Aureococcus anophagefferens])

HSP 1 Score: 575.859 bits (1483), Expect = 0.000e+0
Identity = 273/460 (59.35%), Postives = 354/460 (76.96%), Query Frame = 0

Query:   74 VAASVLSGHEAPSILADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRR------VSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDMAHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRKE---LAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMEL-QRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFP 523
            ++A  +  H     L + D E+  +I+ E+ RQ + IELIASENFAS+A+++ LGS +TNKY+EG PG RYYGGNE +D +E +C++RAL  + L ++ WGVNVQPYSGSPANFAVYT LL+PHDRIMGLDLPSGGHLTHG+Y   ++      VSATS+YFESLPY+V   TGLVD+DEL K+A++F+P LIV GGSAYPRDWDYA+ R IADA G+ LM DMAHISGLV+    N+PF Y D+VT+TTHKSLRGPRSG+IFF+K+     ++++ AVFP+LQGGPH HQIA +AV LKE   P F+  Y+ +VK+N +A+A +L  + G+++ T GT+NHLLLWD+RP GITGSKVEK+ + V I++NKN + GD SA SPGGVR+G PAMTTRGL E+DF  VA  L + ++LALK+QA A SKKLVDF +A++ +  EV ALR++V+ FAS+F  P
Sbjct:    1 MSAMAIKTHPGLKTLEEVDSEMYELIEQEKRRQFTSIELIASENFASRAIMDCLGSCLTNKYAEGLPGKRYYGGNEIIDQIENMCKSRALEAYRLKTDEWGVNVQPYSGSPANFAVYTGLLQPHDRIMGLDLPSGGHLTHGFYTLDKKTMSRKPVSATSVYFESLPYKVHQTTGLVDFDELAKMAAIFKPALIVCGGSAYPRDWDYAKFREIADANGSLLMMDMAHISGLVATQEANDPFQYCDIVTTTTHKSLRGPRSGIIFFKKDARGFEDKINNAVFPALQGGPHEHQIAGVAVQLKETTKPEFK-GYVQQVKKNIKAMAAKLVDQYGYALATGGTDNHLLLWDLRPAGITGSKVEKICDVVQITLNKNAVPGDVSALSPGGVRIGAPAMTTRGLVEKDFEAVADLLHEAVQLALKIQAAAPSKKLVDFSKALEGN-AEVDALREKVKGFASSFGMP 458          

BLAST of EWM29233.1 vs. NCBI_GenBank
Match: gi|676386769|ref|XP_009036663.1| (hypothetical protein AURANDRAFT_53612 [Aureococcus anophagefferens] >gi|323452799|gb|EGB08672.1| hypothetical protein AURANDRAFT_53612 [Aureococcus anophagefferens])

HSP 1 Score: 575.859 bits (1483), Expect = 0.000e+0
Identity = 274/460 (59.57%), Postives = 353/460 (76.74%), Query Frame = 0

Query:   74 VAASVLSGHEAPSILADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRR------VSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDMAHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRKE---LAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMEL-QRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFP 523
            ++A  +  H     L + D E+  +I+ E+ RQ + IELIASENFAS+A+++ LGS +TNKY+EG PG RYYGGNE +D +E +C+ARAL  + L ++ WGVNVQPYSGSPANFAVYT LL+PHDRIMGLDLPSGGHLTHG+Y   ++      VSATS+YFESLPY+V   TGLVD+DEL K+A++F+P LIV GGSAYPRDWDYA+ R IADA G+ LM DMAHISGLV+    N+PF Y D+VT+TTHKSLRGPRSG+IFF+K+     ++++ AVFP+LQGGPH HQIA +AV LKE   P F+  Y+ +VK+N +A+A +L  + G+++ T GT+NHLLLWD+RP GITGSKVEK+ + V I++NKN + GD SA SPGGVR+G PAMTTRGL E+DF  VA  L + ++LALK+QA A SKKLVDF +A+  +  EV ALR++V+ FAS+F  P
Sbjct:    1 MSAMAIKSHPGLKTLEEVDTEMYELIEQEKRRQFTSIELIASENFASRAIMDCLGSCLTNKYAEGLPGKRYYGGNEIIDQIENMCKARALEAYRLKTDEWGVNVQPYSGSPANFAVYTGLLQPHDRIMGLDLPSGGHLTHGFYTLDKKTMSRKPVSATSVYFESLPYKVHQTTGLVDFDELAKMAAIFKPALIVCGGSAYPRDWDYAKFREIADANGSLLMMDMAHISGLVATQEANDPFKYCDIVTTTTHKSLRGPRSGIIFFKKDARGFEDKINNAVFPALQGGPHEHQIAGVAVQLKETTKPEFK-GYVQQVKKNIKAMAAKLVDQYGYALATGGTDNHLLLWDLRPAGITGSKVEKICDVVQITLNKNAVPGDVSALSPGGVRIGAPAMTTRGLVEKDFEAVADLLHEAVQLALKIQAAAPSKKLVDFSKALVGN-AEVDALREKVKGFASSFGMP 458          

BLAST of EWM29233.1 vs. NCBI_GenBank
Match: gi|290987042|ref|XP_002676232.1| (hydroxymethyltransferase [Naegleria gruberi] >gi|284089833|gb|EFC43488.1| hydroxymethyltransferase [Naegleria gruberi])

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 274/436 (62.84%), Postives = 338/436 (77.52%), Query Frame = 0

Query:   88 LADEDPEVAHIIQAERIRQISGIELIASENFASKAVLEALGSVMTNKYSEGRPGARYYGGNEHVDALERLCEARALSLFNLSSESWGVNVQPYSGSPANFAVYTALLEPHDRIMGLDLPSGGHLTHGYYNEKRRVSATSIYFESLPYRVDPVTGLVDYDELQKLASLFRPRLIVAGGSAYPRDWDYARLRAIADACGAYLMTDMAHISGLVSAGACNNPFPYSDVVTSTTHKSLRGPRSGMIFFRKELAEQVHFAVFPSLQGGPHNHQIAALAVALKEAAAPSFRQDYIWRVKRNAEALAMELQRLGHSVVTNGTENHLLLWDMRPHGITGSKVEKLLEAVGISVNKNTIHGDKSASSPGGVRVGTPAMTTRGLTERDFLHVAAFLDDGLRLALKVQAGADSKKLVDFERAMQAHEQEVRALRDRVQHFASNFSFP 523
            L+  DPE+  +I+ E+ RQ  G+ELIASENF S+AV++ LGS +TNKYSEG+ GARYYGGNE++D +E+LC+ RAL  F+L+SE W VNVQPYSGSPANFAVYT LL+PHDRIMGLDLPSGGHLTHGYY+ K+++SATSIYFESLPY VD   GL+DYD L+K A +FRP+LI+ GGSAYPRDWDYARLR IAD   AYLM DMAH SGLV+ G  N+PF Y DVVTSTTHKSLRGPR+G+IF +K L  ++ FAVFP +QGGPHNHQIAA+A  LKE   P F+Q YI +VK NA+ LA  L   G+++ T GT+NHL+LW++RP GITGSK+EKL +AV I+ NKN+I GD +A SP GVR+GTPA+TTRG  E DF  VA FL  G+++ LK+Q  A S KL DF  A+  + +E+  L+  V+ FA  F  P
Sbjct:   15 LSQADPELFDLIEKEKERQWKGLELIASENFTSQAVMDCLGSCLTNKYSEGQVGARYYGGNEYIDEIEKLCKTRALEAFSLNSEDWSVNVQPYSGSPANFAVYTGLLQPHDRIMGLDLPSGGHLTHGYYSGKKKISATSIYFESLPYTVDQ-QGLIDYDGLEKSARVFRPKLIICGGSAYPRDWDYARLRKIADEIEAYLMCDMAHYSGLVATGEHNSPFQYCDVVTSTTHKSLRGPRAGIIFAKKALMPKIDFAVFPGIQGGPHNHQIAAIATQLKEVKTPEFKQ-YIQQVKANAKTLAKALIEKGYTLATGGTDNHLVLWNLRPQGITGSKMEKLFDAVSITSNKNSIAGDANALSPFGVRLGTPALTTRGFKEVDFEKVAEFLHRGVQIGLKLQEQAVSTKLADF-LALFENNEELTQLKSEVESFAKQFGIP 447