EWM29297.1, cds1384 (CDS) Nannochloropsis gaditana

BLAST of EWM29297.1 vs. NCBI_GenBank
Match: gi|585111820|gb|EWM29297.1| (trna guanosine-2 -o-methyltransferase trm13 [Nannochloropsis gaditana])

HSP 1 Score: 1173.69 bits (3035), Expect = 0.000e+0
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query:    1 MEDSKETRTPSEAVIPPSPLTGTDIGLKIRCPPKQDRCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGLNPQPLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEARVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGGLTSPLVFMGKHVCGVATDLSLRAVADYVRDFEGSKEEGAAGEGPCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGGVEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILAWTPINEEEAENFRNVEL 575
            MEDSKETRTPSEAVIPPSPLTGTDIGLKIRCPPKQDRCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGLNPQPLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEARVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGGLTSPLVFMGKHVCGVATDLSLRAVADYVRDFEGSKEEGAAGEGPCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGGVEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILAWTPINEEEAENFRNVEL
Sbjct:    1 MEDSKETRTPSEAVIPPSPLTGTDIGLKIRCPPKQDRCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGLNPQPLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEARVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGGLTSPLVFMGKHVCGVATDLSLRAVADYVRDFEGSKEEGAAGEGPCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGGVEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILAWTPINEEEAENFRNVEL 575          

BLAST of EWM29297.1 vs. NCBI_GenBank
Match: gi|553194716|ref|XP_005856020.1| (coiled-coil domain containing 76, partial [Nannochloropsis gaditana CCMP526] >gi|553194973|ref|XP_005856065.1| coiled-coil domain containing 76, partial [Nannochloropsis gaditana CCMP526] >gi|422292995|gb|EKU20296.1| coiled-coil domain containing 76, partial [Nannochloropsis gaditana CCMP526] >gi|422293037|gb|EKU20338.1| coiled-coil domain containing 76, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 398.282 bits (1022), Expect = 2.659e-134
Identity = 195/195 (100.00%), Postives = 195/195 (100.00%), Query Frame = 0

Query:  102 YERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGLNPQPLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEARVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGG 296
            YERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGLNPQPLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEARVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGG
Sbjct:    1 YERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGLNPQPLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEARVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGG 195          

BLAST of EWM29297.1 vs. NCBI_GenBank
Match: gi|998707407|ref|XP_015491430.1| (PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog [Parus major])

HSP 1 Score: 207.223 bits (526), Expect = 9.883e-57
Identity = 164/536 (30.60%), Postives = 253/536 (47.20%), Query Frame = 0

Query:   33 PKQDRCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSG-----SLRRPGLNPQPLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEARVVL---VERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGGLTSPLVFMGKHVCGVATDLSLRAVADYVRDFEGSKEEGAAGEGPCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGG--VEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILA 558
            P+  RCA F+ RK RFC +   P  ++CG H H                +E++  ++R+PCPLD  HTVYE +L  HL+ CN + + K +    ++ + IN+G      +    +    L ++E+  ++ +++   +  + H +    EQI S    ++ L     KN   ES  +HL QQAS++ ++ +  LLG     VE GAGRG L H V  A      VL   VERA  R K D  +++     +F R ++DI+ L +N +P+       PLV +GKH+CG ATDL+LR + +       S      GEG  E AP+    A   G     +     ++ G  +   G+VIA CC+H C +  YVG + +++ +GLG  EF   QR + W +                            C  +ET ++  GG   ED      + EET+ RT  ++G + + G                LS +++ +IG  CKRLID+GR+ YL+  G +A    Y E  VS EN  + A
Sbjct:   16 PEPGRCAHFVLRKGRFCRMIPAPGRRFCGEHGH----------------EEEQNDRKRIPCPLDPKHTVYEDQLQKHLKKCNSREKPKPV----YFVQDINAGLKDIIEIPEKKVPLSSLSIEELQNLIIKLKKASNGLELHLK----EQILSHQALQEALND--PKN--GESAFKHLKQQASILGNMEKLHLLGPGRCFVEFGAGRGKLSHWVDIALENVESVLFLLVERATTRFKVDGKHKK--RDSIFERLQVDIQHLCLNKVPILEKK-KLPLVGIGKHLCGAATDLALRCLVE-------SYTTCCDGEGE-EPAPKRCRAAQTEGAPHKSA--GNESTTGDHKPVAGIVIALCCHHKCDWTHYVGRDFFKS-VGLGPVEFHYFQRMSSWAT----------------------------CGMQETTSKASGGEGSEDQTNDTEEHEETLSRT--ESGSDTLQGI---------------LSVEERKEIGCLCKRLIDHGRIEYLQHRGYKAALQYYTESAVSLENVLLTA 464          

BLAST of EWM29297.1 vs. NCBI_GenBank
Match: gi|1095986816|ref|XP_009086749.2| (PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog isoform X1 [Serinus canaria])

HSP 1 Score: 207.223 bits (526), Expect = 1.015e-56
Identity = 164/539 (30.43%), Postives = 255/539 (47.31%), Query Frame = 0

Query:   33 PKQDRCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGLNPQ------PLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPE---ARVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGGLTSPLVFMGKHVCGVATDLSLRA-VADYVRDFEGSKEEGAAGEG---PCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGGVEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILA 558
            P+  RCA F+ RK RFC +   P  ++CG H H                +E++  ++R+PCPLD  HTVYE +L  HL+ CN + + K +    ++ + IN+G L+     P+       L ++E+  ++ R++   +  + H +    EQI S    ++ L+    KN   ES  +HL QQAS++ ++ +  LLG     VE GAGRG L H +  A       + +LVERA  R K D  ++R     +F R ++DI+ L +N +P+       P+V +GKH+CG ATDL+LR  V  Y    +G  EE A         E AP    G   + +D               +   G+VIA CC+H C +  YVG E +++ +GLG  EF   QR + W +                     +E+ + +   E++G        D      + E T+ RT+  +G + + G                LS +++ +IG  CKRLID+GR+ YL+  G +A    Y EP VS EN  + A
Sbjct:   14 PEPGRCAHFVLRKKRFCKMIPAPGRRFCGEHGH----------------EEEQNDRKRIPCPLDPKHTVYEDQLQKHLKKCNSREKPKPV----YFVQDINAG-LKDAAEIPEKQVPLSSLSIEELQNLITRLKKATNGLELHLK----EQILSHQALQEALSD--PKN--GESAFKHLKQQASILGNMEKLHLLGPGKCFVEFGAGRGKLSHWLDIALENVESVQFLLVERATTRFKVDGKHKR--RDSIFERLQVDIQHLCLNKVPILEKK-KLPVVGIGKHLCGAATDLALRCLVESYTTCCDGKDEEPAPKRCRAVQTEVAPHKSAGNESITED--------------HKPVAGIVIALCCHHKCDWTHYVGREFFKS-VGLGPVEFHYFQRMSSWATC------------------GMQETTSKASTSEDSG--------DQTNDTEEHEHTLSRTA--SGSDTLQGI---------------LSVEERKEIGCLCKRLIDHGRIEYLQQRGYKAALQYYTEPAVSLENVLLTA 462          

BLAST of EWM29297.1 vs. NCBI_GenBank
Match: gi|291398434|ref|XP_002715882.1| (PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog isoform X1 [Oryctolagus cuniculus])

HSP 1 Score: 205.297 bits (521), Expect = 6.538e-56
Identity = 168/563 (29.84%), Postives = 250/563 (44.40%), Query Frame = 0

Query:   13 AVIPPSPLTGTDIGLKIRCPPKQDRCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGLNPQPLDV-----DEVPAILARIR--------GLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEA---RVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLP-LGPGGLTSPLVFMGKHVCGVATDLSLRAVADYVRDFEGSKEEGAAGEGPCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGGVEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILA 558
            A  P  P TG          P + RC +F+++K RFC +      ++CG H          A AA     E+   ++R+ CPLD  HTVYE +L+ HL+ CN + + K      F+ + IN+G      +  Q + +     +++  ++ ++R         L+H   SHP                 L   +      +S  +HL QQAS++ ++ +  LLG R   VE GAG+G L H V  A  +A     +LVE+   R K D  +R+  +  VF R ++DI+ L +N +P L  G L  P+V +GKH+CGVATDL+LR +   +  +  S EE    E P   A R+    T    + +   R +N    K     G+VIA CC+H C +  YVG E +   LGLG  EF   QR + W +                            C   +T  E        +G +   EE       D GG ++        DD+  SL   L+ ++K +IG  CK LID GR+ YL+  G       Y +P VS EN  + A
Sbjct:    3 ASAPTPPATGF---------PAEGRCGYFVEKKKRFCRMVVAAGKRFCGEH----------AGAA-----EEENTRKRILCPLDPKHTVYEDQLAKHLKKCNAREKPK----PDFFIQDINAGLTDETEILEQLVPISSLSEEQLENLIKKLRKASEGLNSTLKHHIMSHP----------------ALHDALNDPNNGDSATKHLKQQASILGNIEKLKLLGPRRCFVEFGAGKGKLSHWVDIALKDAEKVHFILVEKGTTRFKVDGKHRK--KNSVFERLQIDIQHLCLNKIPVLREGKL--PVVGIGKHLCGVATDLALRCL---IETYAASCEE--RNEEPL--AKRIKNDKTEKEINTLAKERNENIVPEKWTPVAGIVIALCCHHRCDWRHYVGKEYFRT-LGLGAVEFHYFQRMSSWAT----------------------------CGMRKTSLETSTMTTQPKGNQKDDEE------HDDGGYRI-------TDDSADSLPGLLTVEEKKKIGHLCKLLIDQGRVKYLQQKGFSPALQYYTDPLVSLENVLLTA 468          

BLAST of EWM29297.1 vs. NCBI_GenBank
Match: gi|1209967839|ref|XP_021380827.1| (tRNA:m(4)X modification enzyme TRM13 homolog [Lonchura striata domestica] >gi|1208038214|gb|OWK64517.1| tRNA:m(4)X modification enzyme TRM13 [Lonchura striata domestica])

HSP 1 Score: 204.912 bits (520), Expect = 7.541e-56
Identity = 161/534 (30.15%), Postives = 247/534 (46.25%), Query Frame = 0

Query:   37 RCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSG-----SLRRPGLNPQPLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAF---PEARVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGGLTSPLVFMGKHVCGVATDLSLRAVAD-YVRDFEGSKEEGAAGE---GPCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGGVEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILA 558
            RCA F+ RK RFC +   P  ++CG H H                +E++  ++R+PCPLD  HTVYE +L  HL+ CN + + K +    ++ + IN+G      +    +    L ++E+  ++ +++   +  + H +    EQI S    ++ L+    KN   ES  +HL QQAS++ ++ +  LLG     VE GAGRG L H V  A       + +LVERA  R K D  ++R     +F R ++DI+ L +N +P+       P+V +GKH+CG ATDL+LR + + Y    +G  EE A         E AP    G                ++   R+   G+VIA CC+H C +  YVG E +++  GLG  EF   QR + W +                    G +         E G       ED      + E T+ RT+  +G + + G                LS +++ +IG  CKRLID+GR+ YL+  G +A    Y EP VS EN  + A
Sbjct:   12 RCAHFVLRKRRFCRMIPAPGRRFCGEHGH----------------EEEQNDRKRIPCPLDPKHTVYEDQLQKHLKKCNSREKPKPV----YFVQDINAGLKDVVEISEKQVPLSSLSLEELQNLIIKLKKASNGLEVHLK----EQILSHQALQEALSD--PKN--GESAFKHLKQQASILGNMEKLHLLGPGRCFVEFGAGRGKLSHWVDIALENVENVQFLLVERATTRFKVDGKHKR--RDSIFERLQVDIQHLCLNKVPILEKK-KLPVVGIGKHLCGAATDLALRCLVESYTTSCDGEDEEPAPKRCRAAQTEVAPHKSAG--------------NESTTEVRKPVAGIVIALCCHHKCDWTHYVGREFFKST-GLGPVEFHYFQRMSSWAT-------------------CGMQETTSKVSASEDG-------EDQTNDTEEHEHTLSRTA--SGSDTLQGI---------------LSVEERKEIGCLCKRLIDHGRIEYLQQQGYKAAMQYYTEPAVSLENVLLTA 456          

BLAST of EWM29297.1 vs. NCBI_GenBank
Match: gi|327270525|ref|XP_003220040.1| (PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog isoform X2 [Anolis carolinensis])

HSP 1 Score: 204.142 bits (518), Expect = 1.456e-55
Identity = 165/542 (30.44%), Postives = 246/542 (45.39%), Query Frame = 0

Query:   33 PKQDRCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSG------SLRRPGLNPQPLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESK-----ARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEARVV---LVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLP-LGPGGLTSPLVFMGKHVCGVATDLSLRAVAD-YVRDFEGSKEEGAAGEGPCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGGVEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILA 558
            P   RCAFF++RK RFC +      ++CG H                   E+   ++R+PCPLD  HTVYE +L  HL+ CN + + K +    ++ + IN+G      SL    ++   L  +E+  ++ ++R                   S  + ++IL     +N  S+ K      +HL QQAS++ ++ R  LL   +  VE GAGRG L H V  A  +A+ V   LVERA  R K D  + R      F R ++DI+ L +N +P L    L  P+V +GKHVCG ATDL+LR + + Y + ++  +E+ A      ++        T    DE  +    N+         G+VIA CC+H C ++ YVG E +    GLG  EF   +R A W +             LRE  +  R++  D   EE++  E E   EDD G                             D  + SL    + +++ QIGR CK LID+GR+ YL+  G EA    Y +  VS EN  + A
Sbjct:   11 PPPGRCAFFVERKKRFCKMVPAQGRRFCGEH----------------GVHEEEKSRKRIPCPLDPKHTVYEDQLQKHLKKCNSREKPKPV----YFAQDINAGPKDATESLEEEQVSISALSKEELEDLIRKLR-------------RASTSISNVIKDQILCHQALQNALSDPKNGDAATKHLKQQASILGNMERLELLQPGNCFVEFGAGRGKLSHWVDTALHDAKNVHFLLVERAATRFKVDGKHSRNS----FERLQVDIQHLCLNKVPVLAKEKL--PVVGIGKHVCGAATDLALRCLVETYAKHYDNREEDPAPKRLKTDET-----NETSFCPDETCT----NSVSENWSPVAGIVIALCCHHRCDWKHYVGQEFF-TAAGLGATEFNYFRRMASWATC-----------GLRETAR--RDTATDGKNEEQSN-EAEEHEEDDNGA----------------------------DSNWDSLQGLFTAEERKQIGRRCKLLIDHGRIEYLQQRGYEAVLQRYTDLSVSLENVLLTA 461          

BLAST of EWM29297.1 vs. NCBI_GenBank
Match: gi|975106853|ref|XP_015268842.1| (PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog [Gekko japonicus])

HSP 1 Score: 203.756 bits (517), Expect = 2.108e-55
Identity = 164/535 (30.65%), Postives = 252/535 (47.10%), Query Frame = 0

Query:   33 PKQDRCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGL------NPQPLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEAR---VVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGGLTSPLVFMGKHVCGVATDLSLRAVADYVRDFEGSKEEGAAGEGPCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGGVEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILA 558
            P+  RCA+F++RK RFC +      ++CG H                  QE+  G+RR+PCPLDS HTVYE +L  HL+ CN + + K +    ++ + IN+GS     L      +   L  +E+  ++ +++   +  +S  +    +QI S     + L   ++     ++  +HL QQAS++ ++ R  LLG R   VE GAGRG L H V +A  +A+    +LVERA  R K D  +RR      F R ++DI+ L ++ +P+       P+V +GKHVCG ATDL+LR +A+   +     ++G   E P   A R     T    +   S     +S        G+ IA CC+H C ++ YVG E +   +GLG  EF+  +R   W +      G R         ++G ++     E +E G+E E     DR V           S D                   SL   L+ +++ QIG  CK LID+GR+ YL+  G +A    Y +P VS EN  + A
Sbjct:   11 PQPGRCAYFVERKRRFCKMVPARGRRFCGEH----------------AAQEEENGRRRIPCPLDSKHTVYEDQLQKHLKKCNSREKPKPV----YFAKDINAGSKEANKLPEEKQVSISALSKEELEDLIRKLKRASNGIKSVLK----DQICS----HQALQEALSDPRNGDAAFKHLKQQASILGNMERLNLLGPRRCFVEFGAGRGKLSHWVDRALQDAKDVHFLLVERATTRFKVDGKHRRNS----FERLQVDIQHLCLSKVPVLVKE-KLPVVGIGKHVCGSATDLALRCLAESYANL--CDDDGR--ESP--TAKRWKSSGTDGPPNNSPSEMCSKSSVENWSPVAGIAIALCCHHRCDWKHYVGQEFF-TAVGLGVTEFDYFKRMTSWATC-----GMR---------ESGPQTSTIEVESKEQGSEAEEQDPHDRSV---------ECSSD-------------------SLQGLLTTEERKQIGHLCKLLIDHGRIEYLQQRGYDAVLQYYTDPSVSLENVLLTA 463          

BLAST of EWM29297.1 vs. NCBI_GenBank
Match: gi|929439761|ref|XP_005482098.2| (PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog [Zonotrichia albicollis])

HSP 1 Score: 203.371 bits (516), Expect = 2.901e-55
Identity = 164/539 (30.43%), Postives = 246/539 (45.64%), Query Frame = 0

Query:   33 PKQDRCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGLNPQ------PLDVDEVPAILARIRGLQHQKQSHPRGEEVEQITSGPVAEKILATMVAKNWTSESKARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPE---ARVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGGLTSPLVFMGKHVCGVATDLSLRAVAD-YVRDFEGSKEEGAAGE---GPCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGGVEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILA 558
            P+  RCA F+ RK RFC +   P  ++CG H H                +E++  ++R+PCPLD  HTVYE +L  HL+ CN + + K +    ++ + IN+G L+     P+       L ++E+  ++ R++   +  + H +    EQI S    ++ L+    KN   ES  +HL QQAS++ ++ R  LLG     VE GAGRG L H V  A       + +LVERA  R K D  ++R     +F R ++DI+ L +N +P+       P+V +GKH+CG ATDL+LR + + Y    +G  EE A         E AP    G     +D               +   G+VIA CC+H C +  YVG E +++ +GLG  EF   QR + W                             +C  +ET  +     ED        EE     S  A G                +L   LS +++ ++G  CKRLID+GR+ YL+  G +A    Y E  VS EN  + A
Sbjct:   20 PEPGRCAHFVLRKKRFCRMIPAPGRRFCGEHGH----------------EEEQNDRKRIPCPLDPKHTVYEDQLQKHLKKCNSREKPKPV----YFVQDINAG-LKDVSEIPEKQVPLSSLSIEELQNLITRLKKASNGLELHLK----EQILSHQALQEALSD--PKN--GESAFKHLKQQASILGNMERLHLLGPGRCFVEFGAGRGKLSHWVDIALENVESVQFLLVERATTRFKVDGKHKR--RDSIFERLQVDIQHLCLNKVPILEKK-KLPVVGIGKHLCGAATDLALRCLVESYTTCCDGKDEEPAPKRCRAAQTEVAPHKSAGNESTTED--------------HKPVAGIVIALCCHHKCDWTHYVGREFFKS-VGLGPVEFHYFQRMSSWA----------------------------TCGMQETTGKASTS-EDSEDQTNDTEEDEHTLSSTASGSD--------------TLQGTLSVEERKELGCLCKRLIDHGRIEYLQQQGYKAALQYYTEAAVSLENVLLTA 468          

BLAST of EWM29297.1 vs. NCBI_GenBank
Match: gi|532028570|ref|XP_005357243.1| (PREDICTED: tRNA:m(4)X modification enzyme TRM13 homolog [Microtus ochrogaster])

HSP 1 Score: 203.371 bits (516), Expect = 3.499e-55
Identity = 164/558 (29.39%), Postives = 252/558 (45.16%), Query Frame = 0

Query:   12 EAVIPPSPLTGTDIGLKIRCPPKQDRCAFFLKRKLRFCTVPRLPTSKWCGNHQHLEAGYENCAAAARSKTQEKRGGQRRVPCPLDSNHTVYERELSAHLRVCNVKREQKFLEDKPFYRRSINSGSLRRPGLNPQPLDVDEVPAILARIRGLQHQKQSH--PRGEEVEQITSGPVAEKILATMVAKNWTSESK-----ARHLVQQASLISHLHRRGLLGARSTCVELGAGRGTLGHAVHQAFPEA---RVVLVERAGVRLKADAPYRRTGEGGVFARYRLDIRDLWINGLPLGPGGLTSPLVFMGKHVCGVATDLSLRAVAD-YVRDFEGSKEEGAAGEGPCEQAPRLGEGATIVGQDEMGSPRAQNASKGKRRARVGLVIATCCYHVCRYEDYVGVEAWENDLGLGREEFEVAQRSACWVSSLTSARGHRYKPRLREEGKAGRESYADSCEEEETGAEGEGGVEDDRGVRGQREETVRRTSGDAGGEQVGGRRQAEMDDAYTSLGKGLSRDQKIQIGRACKRLIDYGRLAYLRASGLEAECLLYCEPDVSPENSCILA 558
            EA  PPS  T           P + RC++F+++K RFC +      ++CG H          A AA     E+   ++R+ CPLD  HTVYE +L+ HL+ CN + + K      F+ + IN+GS              E+P  L     L  ++  +   +  +  +  + P+ + I++     +  ++ K      +HL QQAS++ ++ +  LLG R   VE GAG+G L H V  A  +A     +LVE+   R K D  +RR  +   F R ++DI+ L +N +P+   G   P+V +GKH+CGVATDL+LR + + Y  +FE   EE  A         R+ +  T    + +    ++  +   R    G+VIA CC+H C +  YVG E ++  LGLG E+F   QR + W +      G R                  S E  +T AE                   R+   +   E+   R     D +  S+   L+ ++K  IG  CK LID GRL YL+  G       Y +P VS EN  + A
Sbjct:    2 EAPAPPSHATAL---------PAEGRCSYFVEKKKRFCRMVAAAGRRFCGEH----------AGAA-----EEENARKRILCPLDPKHTVYEDQLAKHLKKCNSREKPK----PDFFIQDINAGSKHEM----------EIPEQLVPFSSLSEEQLENLIKKLRKASEGLNSPLKDHIMSHPALYDALNDPKNGDCAVKHLKQQASILGNIEKLKLLGPRRCFVEFGAGKGKLSHWVDIALKDAENVHFILVEKVTTRFKVDGKHRR--KNSAFERLQIDIQHLCLNRIPVLREGRL-PVVGIGKHLCGVATDLALRCLVETYAANFEERTEEPLAK--------RVKDDKTEEESNALSKEVSEEDTPETRTPVAGIVIALCCHHRCDWRHYVGREYFKA-LGLGAEDFYYFQRMSSWATC-----GMRRT----------------SLEASDTTAE-------------------RKDDQNYDSEEHDERGDRPADGSTDSVPGVLTAEEKENIGHLCKLLIDQGRLEYLQQKGFSPALKYYTDPSVSLENVLLTA 469