EWM29401.1, cds1263 (CDS) Nannochloropsis gaditana

BLAST of EWM29401.1 vs. NCBI_GenBank
Match: gi|585111930|gb|EWM29401.1| (trna (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Nannochloropsis gaditana])

HSP 1 Score: 954.51 bits (2466), Expect = 0.000e+0
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query:    1 MAGTFIRNRLLPRVAVGLSGGVDSSVAALLLLEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWIGQAPWEMGKKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSSDHPGMLRVDFDVPQRGVALSQTLALYAGERCLGGGAIVEVGPSYYAQGKRLPERRLTWAVSGGKRCVAEARGGEESPRA 463
            MAGTFIRNRLLPRVAVGLSGGVDSSVAALLLLEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWIGQAPWEMGKKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSSDHPGMLRVDFDVPQRGVALSQTLALYAGERCLGGGAIVEVGPSYYAQGKRLPERRLTWAVSGGKRCVAEARGGEESPRA
Sbjct:    1 MAGTFIRNRLLPRVAVGLSGGVDSSVAALLLLEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWIGQAPWEMGKKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSSDHPGMLRVDFDVPQRGVALSQTLALYAGERCLGGGAIVEVGPSYYAQGKRLPERRLTWAVSGGKRCVAEARGGEESPRA 463          

BLAST of EWM29401.1 vs. NCBI_GenBank
Match: gi|698790382|ref|XP_009828223.1| (tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Aphanomyces astaci] >gi|574117071|gb|ETV82554.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Aphanomyces astaci])

HSP 1 Score: 372.859 bits (956), Expect = 4.125e-122
Identity = 207/454 (45.59%), Postives = 258/454 (56.83%), Query Frame = 0

Query:   13 RVAVGLSGGVDSSVAALLL-LEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWIGQAPWEMG---KKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSSD------HPG-----------MLRVDFDVPQRGVALSQTLALYAGE-RCLGGGAIVEVGPSYYAQGKRLPERRLTWAV 444
            +V VG+SGGVDSSVAA LL  ++  EV G++M NWDT+DE G + CP   +      V   +GIP  RVDF + YW +VF P L+EY +G TPNPD+LCNREIKFD F   A+ +VG   IATGHYA+L                       P +  A + + S    L   +D  KDQ+YFLS V   AFQ VLFPLG   K QVR++A  A L TA  KESMGICF+GKR  G+FL +Y+ PR G FVSV T   +   +G  +  T+GQGA++ G   KWFV GK  +   + VAPGT HPAL+ D   V  + FNW+   P+  G       D  +P    PS  S + +FRC YRARYRQPLAGC V+   D      HP             L+VDFDVPQRG+A  Q++ LY  +  C GGGAI   GPSY+   K LP     W V
Sbjct:   30 KVVVGMSGGVDSSVAAYLLHQDKSLEVHGMYMNNWDTSDEEGHATCPADMEYEAVRQVCNDIGIPCTRVDFVQEYWNNVFAPCLDEYAQGFTPNPDVLCNREIKFDAFARHAKHLVGADAIATGHYAQLL----------------------PMHDSAASSTDSDALRLFAAVDTNKDQTYFLSHVSAAAFQQVLFPLGKFTKPQVREIALHANLSTALAKESMGICFIGKRPFGSFLAQYVPPRQGHFVSVETNATMHAHDG-FSAYTIGQGAKVPGQAAKWFVVGKRDQDAAVLVAPGTHHPALFADHTYVHTQAFNWLAPPPFSQGLIQSSVRDDGKPATPPPSHTSATTSFRCLYRARYRQPLAGCTVTIVHDLDATGLHPARHNHQQLSTDTYLKVDFDVPQRGLAPGQSVVLYRQDGLCFGGGAIYCAGPSYWEMRKPLPSPLHDWHV 460          

BLAST of EWM29401.1 vs. NCBI_GenBank
Match: gi|673026708|ref|XP_008867315.1| (tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Aphanomyces invadans] >gi|574477362|gb|ETW04359.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Aphanomyces invadans])

HSP 1 Score: 355.91 bits (912), Expect = 1.215e-115
Identity = 202/449 (44.99%), Postives = 252/449 (56.12%), Query Frame = 0

Query:   13 RVAVGLSGGVDSSVAALLL-LEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWIGQAPWEMGKKHEDRSQPTDRLPS--TASPSVAFRCEYRARYRQPLAGCQVSTSSD------HP---------GMLRVDFDVPQRGVALSQTLALYAGE-RCLGGGAIVEVGPSYYAQGKRLPERRLTW 442
            +V VG+SGGVDSSVAA LL  ++  EV G++M NWD +DE G + CP   +      V + +GI   RVDF + YW +VF P L+EY +G TPNPD+LCNREIKFD F   A+D +G  +IATGHYA+L             P+PSS     P N     G       L    D  KDQ+YFLS V   AFQ V FPLG   K QVR +A AA L TA  KESMGICF+GKR  G FL +Y+ PR G FVSV +  V+   +G  +  T+GQGA++ G   KWFV GK  E   + +APGT HPAL+ D   V    FNW+                P  ++PS    + +  FRC YRARYRQPLA C V+ + D      HP           L+V+FD+PQRG+A  Q+L LY  +  C GGGAI   GPSY+   K LP     W
Sbjct:   28 KVVVGMSGGVDSSVAAYLLHQDKSLEVHGLYMNNWDCSDEEGQATCPADMEYESVRQVCRDIGISCSRVDFVQTYWNNVFAPCLDEYAQGFTPNPDVLCNREIKFDAFANHAKD-IGADFIATGHYAQLL------------PIPSSALPDSPSNGNGVLG-------LFAAADSNKDQTYFLSHVSLAAFQKVRFPLGAHTKPQVRQIASAAKLSTALAKESMGICFIGKRPFGTFLAQYVPPRKGHFVSVESNAVMHEHDG-FSSYTIGQGAKVSGQSIKWFVVGKRDEDAAVLIAPGTHHPALFADHTYVDASAFNWL---------------VPPHQIPSGFADNGTTTFRCFYRARYRQPLAACTVTIAHDLDATSLHPMRQTLPTSSTFLKVEFDIPQRGLAPGQSLVLYRDDGLCFGGGAIYCAGPSYWEMQKPLPSPLYDW 440          

BLAST of EWM29401.1 vs. NCBI_GenBank
Match: gi|669146546|ref|XP_008608835.1| (tRNA methyl transferase [Saprolegnia diclina VS20] >gi|530739660|gb|EQC37902.1| tRNA methyl transferase [Saprolegnia diclina VS20])

HSP 1 Score: 346.28 bits (887), Expect = 4.988e-112
Identity = 197/453 (43.49%), Postives = 257/453 (56.73%), Query Frame = 0

Query:   14 VAVGLSGGVDSSVAALLL-LEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWI-GQAPWEMGKKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSS-------------------DHPGMLRVDFDVPQRGVALSQTLALYAGE-RCLGGGAIVEVGPSYYAQGKRLPERRLTWAV 444
            V VG+SGGVDSSV A LL  ++   V+G++M NWD++DE G + CP   D      V  ++GI   RVDF + YW +VF P L++Y KGLTPNPD+LCNREIKFD F   A   +G  +IATGHYA+L+  P                           G+++    L   +D  KDQSYFLS V    FQ V+FPLG L K +VR++A  A LCTA KK+S+GICF+GKR  G+FL +Y+ PR G+FVSV   + +   +G  +  T+GQGA++ G   KWFV GK  E   +++APGT HPAL+ D + V    FNWI G A                  P+  +   + RC+YRARYRQPLA C +S +S                   D P  LRV FDVPQRGVA +Q+L LY  +  C GGG I+  GPSY+ Q K LP     W++
Sbjct:   27 VVVGMSGGVDSSVTAYLLHQDKSLHVVGLYMNNWDSSDEEGKATCPADMDYELVRQVCDEIGIECARVDFVQEYWNNVFEPCLDQYAKGLTPNPDVLCNREIKFDVFAKHAL-ALGADYIATGHYAQLKRLPD--------------------------GTNA----LYAAVDGNKDQSYFLSHVTGAQFQRVMFPLGGLCKPRVREIAAEANLCTAAKKDSVGICFIGKRSFGSFLSQYVPPRTGSFVSVVDEQQMHAHDG-YSAYTIGQGAKVSGLPAKWFVVGKRDEDAAVFIAPGTHHPALFSDEVFVRASAFNWIQGHA------------------PAALTNDGSLRCQYRARYRQPLAHCTISMASMDDVTSGLSVYQPTAIAPADEP-YLRVQFDVPQRGVAPAQSLVLYEDDGLCYGGGPILGAGPSYWEQQKPLPSPLFDWSM 428          

BLAST of EWM29401.1 vs. NCBI_GenBank
Match: gi|568034006|gb|ETM31693.1| (tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Phytophthora parasitica])

HSP 1 Score: 345.895 bits (886), Expect = 1.756e-111
Identity = 200/461 (43.38%), Postives = 245/461 (53.15%), Query Frame = 0

Query:   14 VAVGLSGGVDSSVAALLLLEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWIGQAPWEMGKKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSSDHP---------------------------GMLRVDFDVPQRGVALSQTLALYAGE-RCLGGGAIVEVGPSYYAQGKRLPERRLTWAVSG 446
            V VG+SGGVDSSVAALLL  QGY V GV+M+NWD +DE G SACP   +  D + V +QLGI    V+  + YW  VF P LE Y++GLTPNPDILCNREIKF  F  +A+ + G  ++ATGHYA LR      P+   GP                       P LC   D+ KDQSYFLS V  RAF +VLFPLG +RK +VR +A   GLCTA KK+S+GICF+GKR    F+ +Y+Q + G F +V  GE + +  G  T  TVGQGAR+ G   KWFV GK      + VA GT +PAL+ D L  +  +FNW+                    LP     +   RC YR RYRQ L  C +S  S+H                              LRVDFD PQRGV   Q L LY  +  C GGG I   G +YY QGK L +    W   G
Sbjct:   55 VVVGMSGGVDSSVAALLLKRQGYHVTGVYMKNWDPSDEEGESACPVDSEYMDVQKVCEQLGIEAQMVNLVQSYWNQVFAPCLEGYEEGLTPNPDILCNREIKFKAFTEYAKKI-GADYVATGHYAALR------PAHGCGP-----------------------PNLCAAEDDTKDQSYFLSSVDGRAFANVLFPLGGMRKTEVRQIAEREGLCTATKKDSVGICFIGKRNFADFIHQYIQRQEGYFYTVD-GEKMHSHLG-FTAYTVGQGARLQGMSAKWFVVGKHKSDHSVIVAEGTRNPALFSDTLFASATEFNWMAGV-----------------LPQELRDTGCMRCFYRVRYRQDLDECTISLVSNHEVRASASKLHDWQPEPLADGEDEESRERSYLRVDFDHPQRGVTPEQALVLYRDDGLCYGGGPIAAAGETYYEQGKPLADDVHDWHRQG 466          

BLAST of EWM29401.1 vs. NCBI_GenBank
Match: gi|970645519|gb|KUF85965.1| (hypothetical protein AM588_10002392 [Phytophthora nicotianae])

HSP 1 Score: 345.895 bits (886), Expect = 2.180e-111
Identity = 200/461 (43.38%), Postives = 245/461 (53.15%), Query Frame = 0

Query:   14 VAVGLSGGVDSSVAALLLLEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWIGQAPWEMGKKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSSDHP---------------------------GMLRVDFDVPQRGVALSQTLALYAGE-RCLGGGAIVEVGPSYYAQGKRLPERRLTWAVSG 446
            V VG+SGGVDSSVAALLL  QGY V GV+M+NWD +DE G SACP   +  D + V +QLGI    V+  + YW  VF P LE Y++GLTPNPDILCNREIKF  F  +A+ + G  ++ATGHYA LR      P+   GP                       P LC   D+ KDQSYFLS V  RAF +VLFPLG +RK +VR +A   GLCTA KK+S+GICF+GKR    F+ +Y+Q + G F +V  GE + +  G  T  TVGQGAR+ G   KWFV GK      + VA GT +PAL+ D L  +  +FNW+                    LP     +   RC YR RYRQ L  C +S  S+H                              LRVDFD PQRGV   Q L LY  +  C GGG I   G +YY QGK L +    W   G
Sbjct:   55 VVVGMSGGVDSSVAALLLKRQGYHVTGVYMKNWDPSDEEGESACPVDSEYLDVQKVCEQLGIEAQMVNLVQSYWNQVFAPCLEGYEEGLTPNPDILCNREIKFKAFTEYAKKI-GADYVATGHYAALR------PAHGCGP-----------------------PNLCAAEDDTKDQSYFLSSVDGRAFANVLFPLGGMRKTEVRQIAEREGLCTATKKDSVGICFIGKRNFADFIHQYIQRQEGYFYTVD-GEKMHSHLG-FTAYTVGQGARLQGMSAKWFVVGKHKSDHSVIVAEGTRNPALFSDTLFASATEFNWMAGV-----------------LPQELRDTGCMRCFYRVRYRQDLDECTISLVSNHEVRASASKLHDWQPEPLADGEDEESRERSYLRVDFDHPQRGVTPEQALVLYRDDGLCYGGGPIAAAGETYYEQGKPLADDVHDWHRQG 466          

BLAST of EWM29401.1 vs. NCBI_GenBank
Match: gi|567945964|gb|ETK71775.1| (tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Phytophthora parasitica] >gi|568004089|gb|ETL78424.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Phytophthora parasitica] >gi|570312140|gb|ETO60088.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Phytophthora parasitica P1976])

HSP 1 Score: 345.895 bits (886), Expect = 2.252e-111
Identity = 200/461 (43.38%), Postives = 245/461 (53.15%), Query Frame = 0

Query:   14 VAVGLSGGVDSSVAALLLLEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWIGQAPWEMGKKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSSDHP---------------------------GMLRVDFDVPQRGVALSQTLALYAGE-RCLGGGAIVEVGPSYYAQGKRLPERRLTWAVSG 446
            V VG+SGGVDSSVAALLL  QGY V GV+M+NWD +DE G SACP   +  D + V +QLGI    V+  + YW  VF P LE Y++GLTPNPDILCNREIKF  F  +A+ + G  ++ATGHYA LR      P+   GP                       P LC   D+ KDQSYFLS V  RAF +VLFPLG +RK +VR +A   GLCTA KK+S+GICF+GKR    F+ +Y+Q + G F +V  GE + +  G  T  TVGQGAR+ G   KWFV GK      + VA GT +PAL+ D L  +  +FNW+                    LP     +   RC YR RYRQ L  C +S  S+H                              LRVDFD PQRGV   Q L LY  +  C GGG I   G +YY QGK L +    W   G
Sbjct:   55 VVVGMSGGVDSSVAALLLKRQGYRVTGVYMKNWDPSDEEGESACPVDSEYMDVQKVCEQLGIEAQMVNLVQSYWNQVFAPCLEGYEEGLTPNPDILCNREIKFKAFTEYAKKI-GADYVATGHYAALR------PAHGCGP-----------------------PNLCAAEDDTKDQSYFLSSVDGRAFANVLFPLGGMRKTEVRQIAEREGLCTATKKDSVGICFIGKRNFADFIHQYIQRQEGYFYTVD-GEKMHSHLG-FTAYTVGQGARLQGMSAKWFVVGKHKSDHSVIVAEGTRNPALFSDTLFASATEFNWMAGV-----------------LPQELRDTGCMRCFYRVRYRQDLDECTISLVSNHEVRASASKLHDWQPEPLADGEDEESRERSYLRVDFDHPQRGVTPEQALVLYRDDGLCYGGGPIAAAGETYYEQGKPLADDVHDWHRQG 466          

BLAST of EWM29401.1 vs. NCBI_GenBank
Match: gi|566008477|gb|ETI31388.1| (tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Phytophthora parasitica P1569] >gi|567974617|gb|ETL25206.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Phytophthora parasitica] >gi|570935918|gb|ETP01175.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Phytophthora parasitica CJ01A1])

HSP 1 Score: 345.51 bits (885), Expect = 2.403e-111
Identity = 200/461 (43.38%), Postives = 245/461 (53.15%), Query Frame = 0

Query:   14 VAVGLSGGVDSSVAALLLLEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWIGQAPWEMGKKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSSDHP---------------------------GMLRVDFDVPQRGVALSQTLALYAGE-RCLGGGAIVEVGPSYYAQGKRLPERRLTWAVSG 446
            V VG+SGGVDSSVAALLL  QGY V GV+M+NWD +DE G SACP   +  D + V +QLGI    V+  + YW  VF P LE Y++GLTPNPDILCNREIKF  F  +A+ + G  ++ATGHYA LR      P+   GP                       P LC   D+ KDQSYFLS V  RAF +VLFPLG +RK +VR +A   GLCTA KK+S+GICF+GKR    F+ +Y+Q + G F +V  GE + +  G  T  TVGQGAR+ G   KWFV GK      + VA GT +PAL+ D L  +  +FNW+                    LP     +   RC YR RYRQ L  C +S  S+H                              LRVDFD PQRGV   Q L LY  +  C GGG I   G +YY QGK L +    W   G
Sbjct:   55 VVVGMSGGVDSSVAALLLKRQGYHVTGVYMKNWDPSDEEGESACPVDSEYLDVQKVCEQLGIEAQMVNLVQSYWNQVFAPCLEGYEEGLTPNPDILCNREIKFKAFTEYAKKI-GADYVATGHYAALR------PAHGCGP-----------------------PNLCAAEDDTKDQSYFLSSVDGRAFANVLFPLGGMRKTEVRQIAEREGLCTATKKDSVGICFIGKRNFADFIHQYIQRQEGYFYTVD-GEKMHSHLG-FTAYTVGQGARLQGMSAKWFVVGKHKSDHSVIVAEGTRNPALFSDTLFASATEFNWMAGV-----------------LPQELRDTGCMRCFYRVRYRQDLDECTISLVSNHEVRASASKLHDWQPEPLADGEDEESRERSYLRVDFDHPQRGVTPEQALVLYRDDGLCYGGGPIAAAGETYYEQGKPLADDVHDWHRQG 466          

BLAST of EWM29401.1 vs. NCBI_GenBank
Match: gi|675179247|ref|XP_008898508.1| (tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Phytophthora parasitica INRA-310] >gi|568101465|gb|ETN16151.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Phytophthora parasitica INRA-310] >gi|970633057|gb|KUF78938.1| tRNA-specific 2-thiouridylase MnmA [Phytophthora nicotianae])

HSP 1 Score: 344.354 bits (882), Expect = 7.729e-111
Identity = 199/461 (43.17%), Postives = 245/461 (53.15%), Query Frame = 0

Query:   14 VAVGLSGGVDSSVAALLLLEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWIGQAPWEMGKKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSSDHP---------------------------GMLRVDFDVPQRGVALSQTLALYAGE-RCLGGGAIVEVGPSYYAQGKRLPERRLTWAVSG 446
            V VG+SGGVDSSVAALLL  QGY V GV+M+NWD +DE G SACP   +  D + V +QLGI    V+  + YW  VF P LE Y++GLTPNPDILCNREIKF  F  +A+ + G  ++ATGHYA LR      P+   GP                       P LC   D+ KDQSYFLS V  RAF +VLFPLG +RK +VR +A   GLCTA KK+S+GICF+GKR    F+ +Y++ + G F +V  GE + +  G  T  TVGQGAR+ G   KWFV GK      + VA GT +PAL+ D L  +  +FNW+                    LP     +   RC YR RYRQ L  C +S  S+H                              LRVDFD PQRGV   Q L LY  +  C GGG I   G +YY QGK L +    W   G
Sbjct:   55 VVVGMSGGVDSSVAALLLKRQGYHVTGVYMKNWDPSDEEGESACPVDSEYLDVQKVCEQLGIEAQMVNLVQSYWNQVFAPCLEGYEEGLTPNPDILCNREIKFKAFTEYAKKI-GADYVATGHYAALR------PAHGCGP-----------------------PNLCAAEDDTKDQSYFLSSVDGRAFANVLFPLGGMRKTEVRQIAEREGLCTATKKDSVGICFIGKRNFADFIHQYIERQEGYFYTVD-GEKMHSHLG-FTAYTVGQGARLQGMSAKWFVVGKHKSDHSVIVAEGTRNPALFSDTLFASATEFNWMAGV-----------------LPQELRDTGCMRCFYRVRYRQDLDECTISLVSNHEVRASASKLHDWQPEPLADGEDEESRERSYLRVDFDHPQRGVTPEQALVLYRDDGLCYGGGPIAAAGETYYEQGKPLADDVHDWHRQG 466          

BLAST of EWM29401.1 vs. NCBI_GenBank
Match: gi|953502975|emb|CEG35743.1| (trna (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Plasmopara halstedii])

HSP 1 Score: 343.584 bits (880), Expect = 1.245e-110
Identity = 205/463 (44.28%), Postives = 249/463 (53.78%), Query Frame = 0

Query:   14 VAVGLSGGVDSSVAALLLLEQGYEVIGVHMQNWDTTDEAGASACPYTEDLRDAEAVSKQLGIPFHRVDFCRPYWTHVFTPFLEEYDKGLTPNPDILCNREIKFDRFQGFARDVVGTPWIATGHYARLRHFPSLGPSLPVGPLPSSTSEGGPRNPRACTGSHSSLPELCTGMDEAKDQSYFLSQVPTRAFQHVLFPLGHLRKGQVRDMAHAAGLCTAEKKESMGICFVGKRRMGAFLPEYLQPRAGTFVSVTTGEVLGTQEGALTLLTVGQGARIGGAEEKWFVCGKDMEAGRIYVAPGTDHPALYCDWLLVAKEDFNWI-GQAPWEMGKKHEDRSQPTDRLPSTASPSVAFRCEYRARYRQPLAGCQVSTSSDHPGM----------------------------LRVDFDVPQRGVALSQTLALYAGE-RCLGGGAIVEVGPSYYAQGKRLPERRLTWAVSG 446
            V VG+SGGVDSSVAALLL  QGY V GV+M+NWD++DE G SACP   + RD + V +QLGI    V+  + YW  VF P L+ Y++GLTPNPDILCNREIKF  F  +A+  +G  ++ATGHYA LR         PV         GG             LP+L    D  KDQSYFLS V  +AF+ VLFPLG +RK QVR +A + GLCTA KK+S+GICF+GKR    F+ +Y+Q + G F ++  G  L    G  T  TVGQGAR+ G   KWFV GK      I++A GT +PALY D L  +   FNWI G  P +         Q   RL          RC YR RYRQ L  C VS  S H  +                            LRVDFD PQRGV   Q+L LY  +  C GGG I   G +YY Q + LP     W   G
Sbjct:   46 VVVGMSGGVDSSVAALLLKRQGYHVTGVYMKNWDSSDEDGVSACPVDLEYRDVQKVCEQLGIEAQMVNMVQSYWNQVFAPCLKGYEEGLTPNPDILCNREIKFKAFTEYAKR-IGADYVATGHYAALR---------PV-------ELGG-------------LPQLFAAEDSTKDQSYFLSNVDGKAFEKVLFPLGGMRKTQVRQIAESEGLCTASKKDSVGICFIGKRNFPDFIHQYIQRQEGYFHAI-NGTKLYPHRG-FTAYTVGQGARLHGMSAKWFVVGKRKSDYSIFIAQGTRNPALYSDTLYASAAQFNWIAGDLPHDF--------QHIGRL----------RCYYRVRYRQDLDACTVSFVSSHDALLNASKFHDWQPELVDDDNLDGKEMTRTYLRVDFDHPQRGVTPDQSLVLYRSDGLCFGGGPIAAAGKTYYEQDRLLPNDAPDWRSQG 458