EWM29563.1, cds1160 (CDS) Nannochloropsis gaditana

BLAST of EWM29563.1 vs. NCBI_GenBank
Match: gi|585112134|gb|EWM29563.1| (Pentatricopeptide repeat contining protein [Nannochloropsis gaditana])

HSP 1 Score: 1964.89 bits (5089), Expect = 0.000e+0
Identity = 952/952 (100.00%), Postives = 952/952 (100.00%), Query Frame = 0

Query:    1 MVNMRSNEAKRSPRSNLRGIFNLGRDARISGLLALLTSSWFLDGVAFHSPSFHSCSRGHARCFTTQPAKKVTMEMVTGHGSSSPIPPARAHPPKRLPGRRPPPSTQHRNLHGGGGLWEINNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVFVRSFFAPREGVSPAEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKAYRDYVVGLDMEGEGGDMAPLPLLVFPPNGGSPLWTQATTPRAVLEAAVHLPSYLQTLAAITPTATAPSPSSSSPHSRAPGAARRSFGTSHLFGGPDSITYNILMDFAGELGDVEALETIHAARNAAGFAIDHYVIHSYMKAHAVRGNLAGVTEAKEKMEALRVPANERTFSVLVLSLLRCGQVEKAELLLKNALARQTYVHDSLFVAFIQHYTSAPSSSQQPSAPEKPTSRLVAAREIFEAFKARRNPGAPMPAAPQQSHGKWAGAVWSAWMAALARKGDLDGVLAAWKEMTLCPSLGVRPTRSTYAQVIRAACVAGEVETALQWVQNMRGKKWVPDVRVYNGLIYACLEKEMGKADRKGSKGGGGQRLLVEVLEMMERDGVQPNAITLRAKEGVMRVLASRMRAVSDTMVGKLKMLALSKQWAERTEGKG 952
            MVNMRSNEAKRSPRSNLRGIFNLGRDARISGLLALLTSSWFLDGVAFHSPSFHSCSRGHARCFTTQPAKKVTMEMVTGHGSSSPIPPARAHPPKRLPGRRPPPSTQHRNLHGGGGLWEINNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVFVRSFFAPREGVSPAEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKAYRDYVVGLDMEGEGGDMAPLPLLVFPPNGGSPLWTQATTPRAVLEAAVHLPSYLQTLAAITPTATAPSPSSSSPHSRAPGAARRSFGTSHLFGGPDSITYNILMDFAGELGDVEALETIHAARNAAGFAIDHYVIHSYMKAHAVRGNLAGVTEAKEKMEALRVPANERTFSVLVLSLLRCGQVEKAELLLKNALARQTYVHDSLFVAFIQHYTSAPSSSQQPSAPEKPTSRLVAAREIFEAFKARRNPGAPMPAAPQQSHGKWAGAVWSAWMAALARKGDLDGVLAAWKEMTLCPSLGVRPTRSTYAQVIRAACVAGEVETALQWVQNMRGKKWVPDVRVYNGLIYACLEKEMGKADRKGSKGGGGQRLLVEVLEMMERDGVQPNAITLRAKEGVMRVLASRMRAVSDTMVGKLKMLALSKQWAERTEGKG
Sbjct:    1 MVNMRSNEAKRSPRSNLRGIFNLGRDARISGLLALLTSSWFLDGVAFHSPSFHSCSRGHARCFTTQPAKKVTMEMVTGHGSSSPIPPARAHPPKRLPGRRPPPSTQHRNLHGGGGLWEINNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVFVRSFFAPREGVSPAEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKAYRDYVVGLDMEGEGGDMAPLPLLVFPPNGGSPLWTQATTPRAVLEAAVHLPSYLQTLAAITPTATAPSPSSSSPHSRAPGAARRSFGTSHLFGGPDSITYNILMDFAGELGDVEALETIHAARNAAGFAIDHYVIHSYMKAHAVRGNLAGVTEAKEKMEALRVPANERTFSVLVLSLLRCGQVEKAELLLKNALARQTYVHDSLFVAFIQHYTSAPSSSQQPSAPEKPTSRLVAAREIFEAFKARRNPGAPMPAAPQQSHGKWAGAVWSAWMAALARKGDLDGVLAAWKEMTLCPSLGVRPTRSTYAQVIRAACVAGEVETALQWVQNMRGKKWVPDVRVYNGLIYACLEKEMGKADRKGSKGGGGQRLLVEVLEMMERDGVQPNAITLRAKEGVMRVLASRMRAVSDTMVGKLKMLALSKQWAERTEGKG 952          

BLAST of EWM29563.1 vs. NCBI_GenBank
Match: gi|1023176431|pdb|5I9G|C (Chain C, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U8c2 In Complex With Its Target Rna U8c2)

HSP 1 Score: 154.836 bits (390), Expect = 1.273e-36
Identity = 105/367 (28.61%), Postives = 180/367 (49.05%), Query Frame = 0

Query:  120 NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEA-ETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVFVRSFFAPREGVSPAE 485
            N  I+   K    D+ L L +++  +G++P++ T++ L++    C+ G+  E L ++++M E  G++P VVTYN L+    G        +AL +  EM + G++PDV+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +  S  + N L D   + G+   AL L+ E          E G    + ++N +I      G ++ A      +  +G K D VTYNTL+D  CKA  L  A  L E+M E  +     T + L+    +      +EAL L   ++   G+K + VT  T+++ +    +  +A+++F        +G+ P E
Sbjct:   55 NTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGL--CKAGKLDEALKLFEEMVE-KGIKPDVVTYNTLID---GLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDG--LCKAGKLDEALKLFEEMV-EKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMV---EKGIKPDE 401          

HSP 2 Score: 135.961 bits (341), Expect = 3.278e-30
Identity = 80/300 (26.67%), Postives = 149/300 (49.67%), Query Frame = 0

Query:  120 NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEA-ETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQE 418
            N  I+   K    D+ L L +++  +G++P++ T++ L++    C+ G+  E L ++++M E  G++P+VVTYN L+    G        +AL +  EM + G++P V+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K D +TY  +V+ +C+A+    AR  + ++ E  +    + +   +++
Sbjct:  160 NTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGL--CKAGKLDEALKLFEEMVE-KGIKPSVVTYNTLID---GLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAYIED 445          

HSP 3 Score: 131.724 bits (330), Expect = 7.228e-29
Identity = 99/351 (28.21%), Postives = 163/351 (46.44%), Query Frame = 0

Query:  182 HGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVF-----------VRSFFAPREGVSPAEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKA 521
             G+ PTVVTYN L+    G        +AL +  EM + G++PDV+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K   VTYNTL+D  CKA  L  A  L E+M E  +  +  T + L+    +      +EAL L   ++   G+K + VT  T+++ +    +  +A+++F           V ++    +G+  A  GK  E  +  +E + +  +P V+      +GL KA
Sbjct:   45 QGVAPTVVTYNTLID---GLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDG--LCKAGKLDEALKLFEEMV-EKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA--GKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA 379          

HSP 4 Score: 117.087 bits (292), Expect = 5.965e-24
Identity = 90/325 (27.69%), Postives = 151/325 (46.46%), Query Frame = 0

Query:  208 RQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVF-----------VRSFFAPREGVSPAEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKA 521
             +AL +  E+R+ GV P V+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K D VTYNTL+D  CKA  L  A  L E+M E  +  +  T + L+    +      +EAL L   ++   G+K + VT  T+++ +    +  +A+++F           V ++    +G+  A  GK  E  +  +E + +  +P V+      +GL KA
Sbjct:   33 ERALELFAELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPSVVTYNTLIDG--LCKAGKLDEALKLFEEMV-EKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA--GKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA 344          

HSP 5 Score: 85.5001 bits (210), Expect = 1.095e-13
Identity = 77/309 (24.92%), Postives = 133/309 (43.04%), Query Frame = 0

Query:  225 DVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVFVRSFFAPREGVSPA------------EGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKA 521
            DV  +  +++     G  + A  L   +  +G+AP   TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K D VTYNTL+D  CKA  L  A  L E+M E  +     T + L+    +      +EAL L   ++   G+K + VT  T+++ +    +  +A+++F        +G+ P+            + GK  E  +  +E + +  +P V+      +GL KA
Sbjct:   15 DVRAYTTVLHALSRAGRYERALELFAELRRQGVAPTVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDG--LCKAGKLDEALKLFEEMV-EKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMV---EKGIKPSVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA 309          

BLAST of EWM29563.1 vs. NCBI_GenBank
Match: gi|1023176429|pdb|5I9F|A (Chain A, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U10 In Complex With Its Target Rna U10)

HSP 1 Score: 149.058 bits (375), Expect = 1.322e-34
Identity = 91/299 (30.43%), Postives = 149/299 (49.83%), Query Frame = 0

Query:  120 NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGE-AETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQ 417
            N  I+   K    D+ L L +++  +G++P++ T++ L++    C+ G+  E L ++++M E  G++P VVTYN L+    G        +AL +  EM + G++PDV+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K D VTYNTL+D  CKA  L  A  L E+M E  +     T   +V+
Sbjct:  125 NTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGL--CKAGKLDEALKLFEEMVE-KGIKPDVVTYNTLID---GLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVE 409          

HSP 2 Score: 137.887 bits (346), Expect = 6.286e-31
Identity = 81/300 (27.00%), Postives = 149/300 (49.67%), Query Frame = 0

Query:  120 NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGE-AETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQE 418
            N  I+   K    D+ L L +++  +G++P++ T++ L++    C+ G+  E L ++++M E  G++P VVTYN L+    G        +AL +  EM + G++PDV+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K D +TY  +V+ +C+A+    AR  + ++ E  +    + +   +++
Sbjct:  160 NTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGL--CKAGKLDEALKLFEEMVE-KGIKPDVVTYNTLID---GLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAYIED 445          

HSP 3 Score: 134.035 bits (336), Expect = 1.499e-29
Identity = 100/351 (28.49%), Postives = 163/351 (46.44%), Query Frame = 0

Query:  182 HGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVF-----------VRSFFAPREGVSPAEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKA 521
             G+ PTVVTYN L+    G        +AL +  EM + G++PDV+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K D VTYNTL+D  CKA  L  A  L E+M E  +     T + L+    +      +EAL L   ++   G+K + VT  T+++ +    +  +A+++F           V ++    +G+  A  GK  E  +  +E + +  +P V+      +GL KA
Sbjct:   45 QGVAPTVVTYNTLID---GLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDG--LCKAGKLDEALKLFEEMV-EKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA--GKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA 379          

BLAST of EWM29563.1 vs. NCBI_GenBank
Match: gi|1023176433|pdb|5I9H|A (Chain A, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U8g2 In Complex With Its Target Rna U8g2)

HSP 1 Score: 144.05 bits (362), Expect = 5.576e-33
Identity = 89/299 (29.77%), Postives = 147/299 (49.16%), Query Frame = 0

Query:  120 NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGE-AETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQ 417
            N  I+   K    D+ L L +++  +G++P++ T++ L++    C+ G+  E L ++++M E  G++P VVTY  L+    G        +AL +  EM + G++PDV+T+  LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K D VTYNTL+D  CKA  L  A  L E+M E  +     T   +V+
Sbjct:  125 NTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGL--CKAGKLDEALKLFEEMVE-KGIKPDVVTYTTLID---GLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVE 409          

HSP 2 Score: 134.806 bits (338), Expect = 8.438e-30
Identity = 80/300 (26.67%), Postives = 147/300 (49.00%), Query Frame = 0

Query:  120 NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGE-AETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQE 418
            N  I+   K    D+ L L +++  +G++P++ T+  L++    C+ G+  E L ++++M E  G++P VVTY  L+    G        +AL +  EM + G++PDV+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K D +TY  +V+ +C+A+    AR  + ++ E  +    + +   +++
Sbjct:  160 NTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGL--CKAGKLDEALKLFEEMVE-KGIKPDVVTYTTLID---GLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAYIED 445          

HSP 3 Score: 129.028 bits (323), Expect = 6.400e-28
Identity = 98/351 (27.92%), Postives = 161/351 (45.87%), Query Frame = 0

Query:  182 HGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVF-----------VRSFFAPREGVSPAEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKA 521
             G+ PTVVTYN L+    G        +AL +  EM + G++PDV+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++  +I      G ++ A      +  +G K D VTY TL+D  CKA  L  A  L E+M E  +     T + L+    +      +EAL L   ++   G+K + VT  T+++ +    +  +A+++F           V ++    +G+  A  GK  E  +  +E + +  +P V+      +GL KA
Sbjct:   45 QGVAPTVVTYNTLID---GLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYTTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDG--LCKAGKLDEALKLFEEMV-EKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA--GKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA 379          

BLAST of EWM29563.1 vs. NCBI_GenBank
Match: gi|1023176427|pdb|5I9D|A (Chain A, Crystal Structure Of Designed Pentatricopeptide Repeat Protein Dppr- U8a2 In Complex With Its Target Rna U8a2)

HSP 1 Score: 140.198 bits (352), Expect = 1.296e-31
Identity = 87/299 (29.10%), Postives = 149/299 (49.83%), Query Frame = 0

Query:  120 NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEA-ETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQ 417
            N  I+   K    D+ L L +++  +G++P++ T++ L++    C+ G+  E L ++++M E  G++P VVTY+ L+    G        +AL +  EM + G++P+V+T++ LI+   + G +  A  L + MV +G+ P+  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K D VTYNTL+D  CKA  L  A  L E+M E  +     T   +V+
Sbjct:  125 NTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGL--CKAGKLDEALKLFEEMVE-KGIKPDVVTYSTLID---GLCKAGKLDEALKLFEEMVEKGIKPNVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVE 409          

HSP 2 Score: 133.265 bits (334), Expect = 2.787e-29
Identity = 79/300 (26.33%), Postives = 148/300 (49.33%), Query Frame = 0

Query:  120 NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEA-ETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQE 418
            N  I+   K    D+ L L +++  +G++P++ T+  L++    C+ G+  E L ++++M E  G++P VVTY+ L+    G        +AL +  EM + G++P+V+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ ++N +I      G ++ A      +  +G K D +TY  +V+ +C+A+    AR  + ++ E  +    + +   +++
Sbjct:  160 NTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYSTLIDGL--CKAGKLDEALKLFEEMVE-KGIKPNVVTYSTLID---GLCKAGKLDEALKLFEEMVEKGIKPNVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDELTYRRVVESYCRAKRFEEARGFLSEVSETDLDFDKKALEAYIED 445          

HSP 3 Score: 127.487 bits (319), Expect = 2.029e-27
Identity = 97/351 (27.64%), Postives = 163/351 (46.44%), Query Frame = 0

Query:  182 HGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVF-----------VRSFFAPREGVSPAEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKA 521
             G+ PTVVTYN L+    G        +AL +  EM + G++PDV+T+N LI+   + G +  A  L + MV +G+ PD  TY TLI G    G L +A +  + M  + +     + N L D   + G+   AL L+ E          E G   D+ +++ +I      G ++ A      +  +G K + VTY+TL+D  CKA  L  A  L E+M E  +     T + L+    +      +EAL L   ++   G+K + VT  T+++ +    +  +A+++F           V ++    +G+  A  GK  E  +  +E + +  +P V+      +GL KA
Sbjct:   45 QGVAPTVVTYNTLID---GLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEM--------VEKGIKPDVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYSTLIDGLCKAGKLDEALKLFEEMVEKGIKPNVVTYNTLIDG--LCKAGKLDEALKLFEEMV-EKGIKPDVVTYNTLIDGLCKAGKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA--GKLDEALKLFEEMVEKGIKPDVVTYNTLIDGLCKA 379          

BLAST of EWM29563.1 vs. NCBI_GenBank
Match: gi|629099602|gb|KCW65367.1| (hypothetical protein EUGRSUZ_G02802 [Eucalyptus grandis] >gi|629099603|gb|KCW65368.1| hypothetical protein EUGRSUZ_G02802 [Eucalyptus grandis] >gi|629099604|gb|KCW65369.1| hypothetical protein EUGRSUZ_G02802 [Eucalyptus grandis])

HSP 1 Score: 138.272 bits (347), Expect = 3.695e-30
Identity = 155/631 (24.56%), Postives = 265/631 (42.00%), Query Frame = 0

Query:  138 LAQKVETEGLR----PNIYTFHALLNAAIACRRGEAE-TLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGA---HGLKQNDVTICTVMEAMLSLERPRDAIQVFVRSFFAPREGVSPA-----EGGKGRERSERGKEAMGRVP---RPRVIPSLVAFNGLIKAY-RDYVV--GLDMEGEGGDMAPLPLLVFPPNGGSPLWTQATTPRAVLEAAVHLPSYLQTLAAITPTATAPS-PSSSSPHSRAPGAARRSFGTSHLFGGPDSITYNILMDFAGELGDVEALETIHAARNAAGFAIDHYVIHSYMKAHAVRGNLAGVTEAKEKMEALRVPANERTFSVLVLSLLRCGQVEKAELLLKNA------LARQTYVHDSLFVAFIQHYTSAPSSSQQ 742
            LA++V ++ LR    PN  T + ++     C   + E  L    KM +E G  P  VTYN +L     RG     ++A  +L +M+  G+ PD  TFN L+    + G +K AA ++ +M+   M PD  TY  LI G   +G L  AF     M    L     + N L D C + G  S A  L  E +        E G  L+  + NI+I  Y     ++ AR A+  +E  G   D VT+NTL+D +CKA NL  A  L+ +M    + +   T++ ++      H +  E+ LD A   L +    G   ++V+  T++      E P  A +++        +G++P+         G  +  +  EAM ++    +  ++P    +N ++  Y R+  V     +     + +  P  VF  N    +         +L+ A+ L S   +   +T   T  +  SS     R   A            GPDS TY  ++    + G  + +E + +    AG   D  +       H V+   A + E  E+++     +N   +S  ++ L    + + A L+ +++      L++  Y+  SL V  I+   S    ++Q
Sbjct:   31 LAREVFSDALRIGVSPNTSTCNIMIYGY--CLENKIEKALEFVNKMVDEFGCLPDNVTYNTMLDALCKRG---RLKEARDLLRDMKDRGLVPDKKTFNILVCGYCKLGRLKEAAQVIDSMMQNDMTPDVWTYNKLISGLCKEGKLDQAFRVKDDMENFKLSPDVVTYNTLIDGCFKWGSSSEAFKLIEEMQ--------EKGVKLNAVTHNIIINLYCKEKKMDEARDAMLKMEENGISPDCVTFNTLIDGNCKANNLEEALRLMHEMGSKGLKMDSFTLNTVL------HALCREKKLDKAYETLCSASKRGYFVDEVSYGTLITGYFDYEMPDKASELWDE---MKEKGITPSIITYNSLMAGLCQFGKTDEAMKKLIELLQSGIVPDETTYNTILNGYCREGCVEKAFQLHNRIVENSFKP-DVFTCN----ILLSGLCKEGMLDKALRLFSTWNSKGKVTDVVTYNTLISSLCKEGRFEEAFDLLVEMELKTKGPDSYTYTAILSALTDAGRRKKVEELMSLMVEAGKLNDFSI-------HEVQAQNAAINEPPEELD-----SNSIAYSEQIIELCSQSKFKDAMLVFEDSKQQGVVLSKIAYI--SLMVGLIKRRKSISRVARQ 620          

HSP 2 Score: 99.3673 bits (246), Expect = 8.447e-18
Identity = 83/378 (21.96%), Postives = 159/378 (42.06%), Query Frame = 0

Query:  183 GLRPTVVTYNILLK-----------------------------------RFFGRGTPAAQRQALAVLNEM-RQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVFV----RSFFAPREGV-SPAEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLI 519
            G+RP  +T N LL                                      +G        +AL  +N+M  + G  PD +T+N +++   + G +K A  L++ M  RG+ PD +T+  L+ G+   G L +A + + +M    +    ++ N L     + G+  +A  +  + E            + D+ ++N +I      G    A   +  ++ +G K + VT+N +++L+CK + +  AR  + KM+EN +     T + L+     A+ +  EEAL L    +G+ GLK +  T+ TV+ A+   ++   A +       R +F       +   G    E  ++  E    +    + PS++ +N L+
Sbjct:    5 GMRPNPLTCNTLLNALVKYPSSHSVLLAREVFSDALRIGVSPNTSTCNIMIYGYCLENKIEKALEFVNKMVDEFGCLPDNVTYNTMLDALCKRGRLKEARDLLRDMKDRGLVPDKKTFNILVCGYCKLGRLKEAAQVIDSMMQNDMTPDVWTYNKLISGLCKEGKLDQAFRVKDDME--------NFKLSPDVVTYNTLIDGCFKWGSSSEAFKLIEEMQEKGVKLNAVTHNIIINLYCKEKKMDEARDAMLKMEENGISPDCVTFNTLIDGNCKANNL--EEALRLMHE-MGSKGLKMDSFTLNTVLHALCREKKLDKAYETLCSASKRGYFVDEVSYGTLITGYFDYEMPDKASELWDEMKEKGITPSIITYNSLM 371          

HSP 3 Score: 91.2781 bits (225), Expect = 3.285e-15
Identity = 83/361 (22.99%), Postives = 157/361 (43.49%), Query Frame = 0

Query:  116 LWEINNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRR--GEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALL---GAHGLKQNDVTICTVMEAMLSLERPRDAIQVFV 471
            +W  N  I+   K+   DQ   +   +E   L P++ T++ L++    C +    +E   + ++M +E G++   VT+NI++  +         R A+    +M + G+ PD +TFN LI+   +  +++ A  LM  M  +G+  D+ T  T++     +  L  A+ET+ +   +   +   S   L         P +A  L+ E +        E G T  + ++N ++      G  + A   L  L + G   D  TYNT+++ +C+   +  A  L  ++ EN+      T +      I+  G+  E  LD A  L     + G   + VT  T++ ++    R  +A  + V
Sbjct:  154 VWTYNKLISGLCKEGKLDQAFRVKDDMENFKLSPDVVTYNTLIDG---CFKWGSSSEAFKLIEEM-QEKGVKLNAVTHNIIINLYCKEKKMDEARDAML---KMEENGISPDCVTFNTLIDGNCKANNLEEALRLMHEMGSKGLKMDSFTLNTVLHALCREKKLDKAYETLCSASKRGYFVDEVSYGTLITGYFDYEMPDKASELWDEMK--------EKGITPSIITYNSLMAGLCQFGKTDEAMKKLIELLQSGIVPDETTYNTILNGYCREGCVEKAFQLHNRIVENSFKPDVFTCN------ILLSGLCKEGMLDKALRLFSTWNSKGKVTDVVTYNTLISSLCKEGRFEEAFDLLV 493          

HSP 4 Score: 83.1889 bits (204), Expect = 1.130e-12
Identity = 62/267 (23.22%), Postives = 122/267 (45.69%), Query Frame = 0

Query:  119 INNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEA----ETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLV 381
             N  I+   K  N ++ L L  ++ ++GL+ + +T + +L+A   CR  +     ETL    K     G     V+Y  L+  +F    P    +A  + +EM++ G+ P ++T+N L+    + G    A   +  ++  G+ PD  TY T++ G+  +G +  AF+    +   +     ++CN+L     + G   +AL L+  + ++        G+  D+ ++N +I +    G  E A   L  +E +    D+ TY  ++
Sbjct:  262 FNTLIDGNCKANNLEEALRLMHEMGSKGLKMDSFTLNTVLHAL--CREKKLDKAYETLCSASK----RGYFVDEVSYGTLITGYFDYEMP---DKASELWDEMKEKGITPSIITYNSLMAGLCQFGKTDEAMKKLIELLQSGIVPDETTYNTILNGYCREGCVEKAFQLHNRIVENSFKPDVFTCNILLSGLCKEGMLDKALRLFSTWNSK--------GKVTDVVTYNTLISSLCKEGRFEEAFDLLVEMELKTKGPDSYTYTAIL 511          

BLAST of EWM29563.1 vs. NCBI_GenBank
Match: gi|702423392|ref|XP_010067268.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g16880 [Eucalyptus grandis] >gi|702423397|ref|XP_010067270.1| PREDICTED: pentatricopeptide repeat-containing protein At2g16880 [Eucalyptus grandis] >gi|702423401|ref|XP_010067271.1| PREDICTED: pentatricopeptide repeat-containing protein At2g16880 [Eucalyptus grandis] >gi|702423406|ref|XP_010067272.1| PREDICTED: pentatricopeptide repeat-containing protein At2g16880 [Eucalyptus grandis] >gi|702423410|ref|XP_010067273.1| PREDICTED: pentatricopeptide repeat-containing protein At2g16880 [Eucalyptus grandis] >gi|702423415|ref|XP_010067274.1| PREDICTED: pentatricopeptide repeat-containing protein At2g16880 [Eucalyptus grandis] >gi|1091489934|ref|XP_018715054.1| PREDICTED: pentatricopeptide repeat-containing protein At2g16880 [Eucalyptus grandis] >gi|1091489940|ref|XP_018715055.1| PREDICTED: pentatricopeptide repeat-containing protein At2g16880 [Eucalyptus grandis])

HSP 1 Score: 139.043 bits (349), Expect = 4.654e-30
Identity = 168/682 (24.63%), Postives = 279/682 (40.91%), Query Frame = 0

Query:   91 HPPKRLPGRRPPPSTQHRNLHGGGGLWEINNEINFWSKQRNFDQVLALAQKVETEGLR----PNIYTFHALLNAAIACRRGEAE-TLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGA---HGLKQNDVTICTVMEAMLSLERPRDAIQVFVRSFFAPREGVSPA------------EGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKAY-RDYVV--GLDMEGEGGDMAPLPLLVFPPNGGSPLWTQATTPRAVLEAAVHLPSYLQTLAAITPTATAPS-PSSSSPHSRAPGAARRSFGTSHLFGGPDSITYNILMDFAGELGDVEALETIHAARNAAGFAIDHYVIHSYMKAHAVRGNLAGVTEAKEKMEALRVPANERTFSVLVLSLLRCGQVEKAELLLKNA------LARQTYVHDSLFVAFIQHYTSAPSSSQQ 742
            H  KR  G RP P T              N  +N   K  +   VL LA++V ++ LR    PN  T + ++     C   + E  L    KM +E G  P  VTYN +L     RG     ++A  +L +M+  G+ PD  TFN L+    + G +K AA ++ +M+   M PD  TY  LI G   +G L  AF     M    L     + N L D C + G  S A  L  E +        E G  L+  + NI+I  Y     ++ AR A+  +E  G   D VT+NTL+D +CKA NL  A  L+ +M    + +   T++       + H +  E+ LD A   L +    G   ++V+  T++      E P  A +++        +G++P+            + GK  E  ++  E +    +  ++P    +N ++  Y R+  V     +     + +  P  VF  N    +         +L+ A+ L S   +   +T   T  +  SS     R   A            GPDS TY  ++    + G  + +E + +    AG   D  +       H V+   A + E  E+++     +N   +S  ++ L    + + A L+ +++      L++  Y+  SL V  I+   S    ++Q
Sbjct:  156 HKMKR-SGMRPNPLT-------------CNTLLNALVKYPSSHSVL-LAREVFSDALRIGVSPNTSTCNIMIYGY--CLENKIEKALEFVNKMVDEFGCLPDNVTYNTMLDALCKRGR---LKEARDLLRDMKDRGLVPDKKTFNILVCGYCKLGRLKEAAQVIDSMMQNDMTPDVWTYNKLISGLCKEGKLDQAFRVKDDMENFKLSPDVVTYNTLIDGCFKWGSSSEAFKLIEEMQ--------EKGVKLNAVTHNIIINLYCKEKKMDEARDAMLKMEENGISPDCVTFNTLIDGNCKANNLEEALRLMHEMGSKGLKMDSFTLN------TVLHALCREKKLDKAYETLCSASKRGYFVDEVSYGTLITGYFDYEMPDKASELWDE---MKEKGITPSIITYNSLMAGLCQFGKTDEAMKKLIELL----QSGIVPDETTYNTILNGYCREGCVEKAFQLHNRIVENSFKP-DVFTCN----ILLSGLCKEGMLDKALRLFSTWNSKGKVTDVVTYNTLISSLCKEGRFEEAFDLLVEMELKTKGPDSYTYTAILSALTDAGRRKKVEELMSLMVEAGKLNDFSI-------HEVQAQNAAINEPPEELD-----SNSIAYSEQIIELCSQSKFKDAMLVFEDSKQQGVVLSKIAYI--SLMVGLIKRRKSISRVARQ 777          

BLAST of EWM29563.1 vs. NCBI_GenBank
Match: gi|747093257|ref|XP_011094425.1| (pentatricopeptide repeat-containing protein At5g12100, mitochondrial [Sesamum indicum])

HSP 1 Score: 134.035 bits (336), Expect = 1.895e-28
Identity = 128/571 (22.42%), Postives = 236/571 (41.33%), Query Frame = 0

Query:  136 LALAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVF---VRSFFAPREGVSP--AEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKAYRDYVVGLDMEGEGGDMAPLPLLVFPPNGGSPLWTQAT-----TPRAVLEAAVHLPSYLQTLAAITPTATAPSPSSSSPHSRAPGAARRSFG-TSHLFGGPDSITYNILMDFAGELGDVEALETIHAARNAAGFAIDHYVIHSYMKAHAVRGNLAGVTEAKEKMEALRVPANERTFSVLV 695
            L L  +++  G RPN + ++ L+      RR + +   ++ +M + + + P  VTYN L+    G         A  +  +M+   VEP+++T+N L+    + G M+ A  +++ M   G  PD  TY+ L+ GH  +GN+  +         + + ++ Y+C++L +   + G+  RA     EF  +  E G      +     N M+  Y  +G+V+ A  A+  +E  G K   +TYNT++   C    +  A   + KM    +    QT + LV      H   +E+ L +    +  +GLK N VT  +++ +     R  +A+ +F   +     P   V     +G   R   +   + +  + R  + P++V +N L+        GL  +G   +   L   +        + T  +     +    LE  + L   ++T + I PT +   P  S           +     SH    PD I YN L+    + G+++    +       G + D    HS +      GN+ G  +  + M A  V  N+  F+ L+
Sbjct:  176 LDLMTQMKKCGARPNGFVYNVLIGGLCKERRVD-DAKKLFDEMLKRN-VAPNRVTYNSLID---GCCKAGDLEGAFNLREKMKTDSVEPNIVTYNTLLGGLCKMGRMEEADKILEEMGAYGFVPDGFTYSILLDGHSRRGNVEASMALYDDAVKKGVTMNEYTCSILMNGLCKLGKMDRA----EEFLMKLKEHGVGLTEVI----LNTMVNGYFKVGYVDKALLAIEKMENEGVKPSCITYNTVISNFCDLGRMDDANEWVRKMNVKGLCPNIQTYNILVDG--YGHSCQFEKCLQILEE-MENNGLKPNVVTYGSLINSFCKKGRLPEAVVIFKDMLNRSVLPNVQVYNMLIDGFCMRGNIDDAFKVLDEMLRSDISPTIVTYNALVN-------GLSKKGRVAEAEELAFSITDKGLNPDVITYNSLICGFSDAGNLEKCLELYEKMKT-SGIKPTLSTYHPLISVCKKDGLDLLEKIVAEMSHWNLSPDRIVYNELIYCYVKQGNIQKATVLCTEMLTLGISPDKMTYHSLIMGRLKEGNVQGARDLFDDMMAKGVAPNDGIFNTLI 722          

HSP 2 Score: 118.242 bits (295), Expect = 2.014e-23
Identity = 92/364 (25.27%), Postives = 157/364 (43.13%), Query Frame = 0

Query:  113 GGGLWEI--NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTM-RAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQ----------EEIIAHGVSWEEALD--LATALLGAHGLKQNDVTICTVM-EAMLSL 460
            G GL E+  N  +N + K    D+ L   +K+E EG++P+  T++ +++    C  G  +    W +     GL P + TYNIL+    G G      + L +L EM   G++P+V+T+  LIN   + G +  A  + + M++R + P+ + Y  LI G  ++GN+ DAF+ +  M R+   P                                             + ++N ++      G V  A     ++  +G   D +TYN+L+     A NL     L EKMK + +  T  T HPL+           E+I+A    W  + D  +   L+  + +KQ ++   TV+   ML+L
Sbjct:  361 GVGLTEVILNTMVNGYFKVGYVDKALLAIEKMENEGVKPSCITYNTVISNF--CDLGRMDDANEWVRKMNVKGLCPNIQTYNILVD---GYGHSCQFEKCLQILEEMENNGLKPNVVTYGSLINSFCKKGRLPEAVVIFKDMLNRSVLPNVQVYNMLIDGFCMRGNIDDAFKVLDEMLRSDISP--------------------------------------------TIVTYNALVNGLSKKGRVAEAEELAFSITDKGLNPDVITYNSLICGFSDAGNLEKCLELYEKMKTSGIKPTLSTYHPLISVCKKDGLDLLEKIVAEMSHWNLSPDRIVYNELIYCY-VKQGNIQKATVLCTEMLTL 674          

HSP 3 Score: 109.768 bits (273), Expect = 7.604e-21
Identity = 80/289 (27.68%), Postives = 136/289 (47.06%), Query Frame = 0

Query:  132 FDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEA-ETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALP--LSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQ 417
            F++ L + +++E  GL+PN+ T+ +L+N+   C++G   E + +++ M     L P V  YN+L+  F  RG       A  VL+EM +  + P ++T+N L+N   + G +  A  L  ++  +G+ PD  TY +LI G    GNL    E  + M+   +   LSTY  + L   C + G     L L  +  AE         R +    +N +I  Y   G+++ A      +   G   D +TY++L+    K  N+ GAR L + M    V       + L++
Sbjct:  452 FEKCLQILEEMENNGLKPNVVTYGSLINSF--CKKGRLPEAVVIFKDMLNRSVL-PNVQVYNMLIDGFCMRGNI---DDAFKVLDEMLRSDISPTIVTYNALVNGLSKKGRVAEAEELAFSITDKGLNPDVITYNSLICGFSDAGNLEKCLELYEKMKTSGIKPTLSTY--HPLISVCKKDG-----LDLLEKIVAEMSHWNLSPDRIV----YNELIYCYVKQGNIQKATVLCTEMLTLGISPDKMTYHSLIMGRLKEGNVQGARDLFDDMMAKGVAPNDGIFNTLIE 723          

HSP 4 Score: 87.4261 bits (215), Expect = 7.784e-14
Identity = 129/651 (19.82%), Postives = 254/651 (39.02%), Query Frame = 0

Query:  138 LAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRF-FGRGTPAA-----QRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVFVRSFFAPREGVSP--------AEGGKGRERSERGKEAMGRVPRPRVIPSLVAFNGLIKAY-------RDYVVGLDMEGEGGDMAPLPLLVFPPNGGSPLWTQATTPRAVLEAAVHLPSYLQTLAAITPTATAPSP--SSSSPHSRAPGAARRSFGTSHLFGGPDSITYNILMDFAGELGDVEA----------------------------------LETIHAARNAAGFAIDHYVIHSYMKAHAVRGNLAGVTEAKEKMEALRVPANERTFSVLVLSLLRCGQVEKAELLLKNALARQTYVHDSLFVAFIQ 731
            L   V ++G  P++  F+  L A +   + + +TL ++             V   + + +F +G+   +A      ++ L ++ +M++ G  P+   +N LI    +   +  A  L   M+ R +AP+  TY +LI G    G+L  AF   + M+  ++  +  + N L     + G+   A  +  E         G  G   D  +++I++  +   G+VE + A      ++G   +  T + L++  CK   +  A   + K+KE+ V +T   ++ +V        V + +   LA   +   G+K + +T  TV+     L R  DA + +VR      +G+ P         +G     + E+  + +  +    + P++V +  LI ++          V+  DM         L   V P      +       R  ++ A  +   +   + I+PT    +   +  S   R   A   +F  +     PD ITYN L+    + G++E                                   LE I A  +    + D  V +  +  +  +GN+   T    +M  L +  ++ T+  L++  L+ G V+ A  L  + +A+    +D +F   I+
Sbjct:  108 LYSLVRSDGNFPSLPAFNLFLEALVTSSQFD-KTLKIFSD----------AVDSGVWVDKFSYGKAIQSAVKLGDLKKGLDLMTQMKKCGARPNGFVYNVLIGGLCKERRVDDAKKLFDEMLKRNVAPNRVTYNSLIDGCCKAGDLEGAFNLREKMKTDSVEPNIVTYNTLLGGLCKMGRMEEADKILEEM--------GAYGFVPDGFTYSILLDGHSRRGNVEASMALYDDAVKKGVTMNEYTCSILMNGLCKLGKMDRAEEFLMKLKEHGVGLTEVILNTMVNG---YFKVGYVDKALLAIEKMENEGVKPSCITYNTVISNFCDLGRMDDANE-WVRKMNV--KGLCPNIQTYNILVDGYGHSCQFEKCLQILEEMENNGLKPNVVTYGSLINSFCKKGRLPEAVVIFKDM---------LNRSVLPNVQVYNMLIDGFCMRGNIDDAFKVLDEMLR-SDISPTIVTYNALVNGLSKKGRVAEAEELAFSITDKGLNPDVITYNSLICGFSDAGNLEKCLELYEKMKTSGIKPTLSTYHPLISVCKKDGLDLLEKIVAEMSHWNLSPDRIVYNELIYCYVKQGNIQKATVLCTEMLTLGISPDKMTYHSLIMGRLKEGNVQGARDLFDDMMAKGVAPNDGIFNTLIE 723          

HSP 5 Score: 70.0922 bits (170), Expect = 1.455e-8
Identity = 49/177 (27.68%), Postives = 91/177 (51.41%), Query Frame = 0

Query:  120 NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEA----ETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQAL 292
            N  +N  SK+    +   LA  +  +GL P++ T+++L+     C   +A    + L +++KM +  G++PT+ TY+ L+      G    ++    ++ EM    + PD + +NELI   V+ G+++ A  L   M+  G++PD  TY +LI G   +GN+  A +    M A+ +
Sbjct:  545 NALVNGLSKKGRVAEAEELAFSITDKGLNPDVITYNSLI-----CGFSDAGNLEKCLELYEKM-KTSGIKPTLSTYHPLISVCKKDGLDLLEK----IVAEMSHWNLSPDRIVYNELIYCYVKQGNIQKATVLCTEMLTLGISPDKMTYHSLIMGRLKEGNVQGARDLFDDMMAKGV 711          

BLAST of EWM29563.1 vs. NCBI_GenBank
Match: gi|1035934520|gb|OAY63523.1| (Pentatricopeptide repeat-containing protein [Ananas comosus])

HSP 1 Score: 131.724 bits (330), Expect = 4.478e-28
Identity = 91/281 (32.38%), Postives = 143/281 (50.89%), Query Frame = 0

Query:  137 ALAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALP--LSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGA---RSLIEKMKENAVPVTHQTI 412
            AL +++ + GLRPN+ TF+ L++    C+ G + +   +    +  GL P V+TYN L+    G G      +AL ++ E+      P+V+TFN LIN   + G +  A GL+  M+ R + P   TY TLI G+   G + DA E  + M  + +   +STY+C +L     R G    A  L  E         GE G   DL ++N++I A   +G +  A   +  +   G +   VTYNTL+D +CK  N+ GA   R+ +EK K++A  VT+  +
Sbjct:  284 ALLREMVSSGLRPNLVTFNTLIDGY--CKDGNSSSAMRFFDEMKRQGLCPNVITYNSLISGLCGEGK---MDEALGLMKEIEGFNAVPNVITFNGLINGFCKKGMLVEANGLLDDMMKRKIEPSVVTYNTLIDGYCRCGVIKDAVELKRVMGEKGISPNVSTYNCLILGFC--RKGDMKSAAKLLDEM--------GEKGIRADLVTYNVLISALCQIGELGKAVKLVDEMFDVGLQPSHVTYNTLIDGYCKKGNIRGALNMRARMEKGKKHANVVTYNVL 549          

HSP 2 Score: 112.079 bits (279), Expect = 7.324e-22
Identity = 78/309 (25.24%), Postives = 146/309 (47.25%), Query Frame = 0

Query:  116 LWEINNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHG 424
            L   N  I+ + K  N    +    +++ +GL PN+ T+++L++    C  G+ +      K  E     P V+T+N L+  F  +G      +A  +L++M +  +EP V+T+N LI+     G +K A  L + M  +G++P+  TY  LI G   KG++  A + +  M  + +     + NVL  A  + G+  +A+ L  E          + G      ++N +I  Y   G++ GA      +E+    A+ VTYN L+   C+   L  A  L+ +M E  + V ++  + ++++ ++  G
Sbjct:  298 LVTFNTLIDGYCKDGNSSSAMRFFDEMKRQGLCPNVITYNSLISG--LCGEGKMDEALGLMKEIEGFNAVPNVITFNGLINGFCKKGMLV---EANGLLDDMMKRKIEPSVVTYNTLIDGYCRCGVIKDAVELKRVMGEKGISPNVSTYNCLILGFCRKGDMKSAAKLLDEMGEKGIRADLVTYNVLISALCQIGELGKAVKLVDEM--------FDVGLQPSHVTYNTLIDGYCKKGNIRGALNMRARMEKGKKHANVVTYNVLIKYFCRKGKLAEANELLNEMLEKGL-VPNRITYEIIKDGMVEKG 592          

HSP 3 Score: 111.694 bits (278), Expect = 1.159e-21
Identity = 84/318 (26.42%), Postives = 140/318 (44.03%), Query Frame = 0

Query:  139 AQKVETEGLR----PNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKG-----------------------------HGL------KGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQ 417
            A+ V  E +R    PN+ TF+  +N    C+ G+        K     G  P+V+TYN L+  +  +G      +A A+L EM   G+ P+++TFN LI+   + G+  +A      M  +G+ P+  TY +LI G                             +GL      KG L++A   +  M  + +  S  + N L D   RCG    A+ L R          GE G + ++ ++N +I  +   G ++ A   L  +  +G +AD VTYN L+   C+   LG A  L+++M +  +  +H T + L+ 
Sbjct:  209 AETVYKEMMRRKIGPNLITFNTAINGL--CKSGKLRRAEDLIKDMRTWGFVPSVITYNTLIDGYCKKGRAGKMFKADALLREMVSSGLRPNLVTFNTLIDGYCKDGNSSSAMRFFDEMKRQGLCPNVITYNSLISGLCGEGKMDEALGLMKEIEGFNAVPNVITFNGLINGFCKKGMLVEANGLLDDMMKRKIEPSVVTYNTLIDGYCRCGVIKDAVELKRVM--------GEKGISPNVSTYNCLILGFCRKGDMKSAAKLLDEMGEKGIRADLVTYNVLISALCQIGELGKAVKLVDEMFDVGLQPSHVTYNTLID 516          

HSP 4 Score: 80.1073 bits (196), Expect = 1.014e-11
Identity = 91/387 (23.51%), Postives = 160/387 (41.34%), Query Frame = 0

Query:  177 KMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGH---GLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREF---------------------EAEADEAGG-----EAGRTL-DLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALL---GAHGLKQNDVTICTVMEAMLSLERPRDAIQVFVRSFFAPREGVSPAE-------GGKGRERSERGKEAM-GRVPRPRVIPSLVAFNGLIKAY 522
            + + ++G R +V++ N LL      G       A  V  EM +  + P+++TFN  IN   + G ++ A  L++ M   G  P   TY TLI G+   G  G +  A   ++ M +  L  +  + N L D   + G  S A+  + E                      E + DEA G     E    + ++ +FN +I  +   G +  A   L  + +R  +   VTYNTL+D +C+   +  A  L   M E  +     T + L+       G   +  +  A  LL   G  G++ + VT   ++ A+  +     A+++    F     G+ P+         G  ++ + RG   M  R+ + +   ++V +N LIK +
Sbjct:  179 RCSADYGCRLSVLSVNPLLSALIDEGEIDG---AETVYKEMMRRKIGPNLITFNTAINGLCKSGKLRRAEDLIKDMRTWGFVPSVITYNTLIDGYCKKGRAGKMFKADALLREMVSSGLRPNLVTFNTLIDGYCKDGNSSSAMRFFDEMKRQGLCPNVITYNSLISGLCGEGKMDEALGLMKEIEGFNAVPNVITFNGLINGFCKKGMLVEANGLLDDMMKRKIEPSVVTYNTLIDGYCRCGVIKDAVELKRVMGEKGISPNVSTYNCLIL------GFCRKGDMKSAAKLLDEMGEKGIRADLVTYNVLISALCQIGELGKAVKLVDEMFDV---GLQPSHVTYNTLIDGYCKKGNIRGALNMRARMEKGKKHANVVTYNVLIKYF 553          

BLAST of EWM29563.1 vs. NCBI_GenBank
Match: gi|242033891|ref|XP_002464340.1| (pentatricopeptide repeat-containing protein At3g53700, chloroplastic [Sorghum bicolor] >gi|241918194|gb|EER91338.1| hypothetical protein SORBI_3001G188600 [Sorghum bicolor])

HSP 1 Score: 131.339 bits (329), Expect = 1.235e-27
Identity = 88/332 (26.51%), Postives = 155/332 (46.69%), Query Frame = 0

Query:  126 WSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLG---AHGLKQNDVTICTVM 454
            + ++ + +  L +  K+   G  P   T + L+N    C+ G  E    + +     G  P  VTYN  +      G  +    AL V++ M Q G +PDV T+N +IN   + G++  A G++  MV RG  PDT T+ TLI     +  L +A +  + +  + L    Y+ N+L +A  + G P   + L+ E ++        +G T D  ++NI+I     +G +  A   L+ +E  G    TVTYNT++D  CK   +  A  + ++M    +  +  T + L+       G+   + +D AT L+      GL+ N++T  +++
Sbjct:  242 FIEEGSIEAALRVKAKMMEAGCSPTGVTVNVLINGY--CKMGRVEDALGYIQQEIADGFEPDQVTYNTFVHGLCQNGHVS---HALKVMDLMLQEGHDPDVFTYNTVINCLSKNGELDEAKGIVNQMVDRGCLPDTTTFNTLIVALSSQNRLEEALDLARELTVKGLSPDVYTFNILINALCKVGDPHLGIRLFEEMKS--------SGCTPDEVTYNILIDHLCSMGKLGNALDLLNEMESNGCPRSTVTYNTIIDALCKKMRIEEAEEVFDQMDAQGISRSAVTFNTLID------GLCKAKRIDDATELIEQMVKEGLQPNNITYNSIL 554          

HSP 2 Score: 105.145 bits (261), Expect = 1.938e-19
Identity = 81/302 (26.82%), Postives = 133/302 (44.04%), Query Frame = 0

Query:  116 LWEINNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQ 417
            ++  N  IN  SK    D+   +  ++   G  P+  TF+ L+ A  +  R E E L + +++T + GL P V T+NIL+      G P      + +  EM+  G  PD +T+N LI+     G +  A  L+  M   G    T TY T+I     K  + +A E    M AQ +  S  + N L D   +  +   A  L  +   E  +            ++N ++  Y   G+++ A   L  +   GF+ D VTY TL++  CKA     A  L+  M+   +  T +  +P++Q
Sbjct:  337 VFTYNTVINCLSKNGELDEAKGIVNQMVDRGCLPDTTTFNTLIVALSSQNRLE-EALDLARELTVK-GLSPDVYTFNILINALCKVGDP---HLGIRLFEEMKSSGCTPDEVTYNILIDHLCSMGKLGNALDLLNEMESNGCPRSTVTYNTIIDALCKKMRIEEAEEVFDQMDAQGISRSAVTFNTLIDGLCKAKRIDDATELIEQMVKEGLQPNN--------ITYNSILTHYCKQGNIKKAADILETMTANGFEIDVVTYGTLINGLCKAGRTQVALKLLRGMRIKGIRPTPKAYNPVIQ 625          

HSP 3 Score: 92.4337 bits (228), Expect = 1.654e-15
Identity = 86/342 (25.15%), Postives = 152/342 (44.44%), Query Frame = 0

Query:  132 FDQVLALAQKVETEGLRPNIYTFHALLNAAIACRR-GEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQAL-AVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFET-VKTMRAQALPLSTYSCNVLADACIRCGQPSRALALYREFEAEADEAGGEAGRTLDLKSFNIMIKAYRGLGHVEGARAALHALERRGFKADTVTYNTLVDLHCKAQNLGGARSLIEKMKENAVPVTHQTIHPLVQEEIIAHGVSWEEALDLATALLGAHGLKQNDVTICTVMEAMLSLERPRDAIQVF 470
            FD +  L +++  EG +       + + +    RR  +A  L + Q   +  G++   V +N LL          ++ + L +V NEM   G++PDV+T N LI        ++ A  +++ M   G+APD  T+TTL++G   +G++  A     K M A   P +  + NVL +   + G+   AL   ++  A+  E         D  ++N  +      GHV  A   +  + + G   D  TYNT+++   K   L  A+ ++ +M +        T + L+    ++     EEALDLA   L   GL  +  T   ++ A+  +  P   I++F
Sbjct:  105 FDLMEGLVREMRREGHQVRAVVVRSFVESYARLRRFDDAVDLVLNQLDNDTFGVQADTVVFNHLLNVL----VEGSKLKLLESVYNEMTGRGIQPDVVTLNTLIKALCRAHQVRTAVLMLEEMSSHGVAPDETTFTTLMQGFIEEGSIEAALRVKAKMMEAGCSP-TGVTVNVLINGYCKMGRVEDALGYIQQEIADGFEP--------DQVTYNTFVHGLCQNGHVSHALKVMDLMLQEGHDPDVFTYNTVINCLSKNGELDEAKGIVNQMVDRGCLPDTTTFNTLIVA--LSSQNRLEEALDLARE-LTVKGLSPDVYTFNILINALCKVGDPHLGIRLF 430          

HSP 4 Score: 69.707 bits (169), Expect = 1.891e-8
Identity = 47/195 (24.10%), Postives = 91/195 (46.67%), Query Frame = 0

Query:  120 NNEINFWSKQRNFDQVLALAQKVETEGLRPNIYTFHALLNAAIACRRGEAETLAVWQKMTEEHGLRPTVVTYNILLKRFFGRGTPAAQRQALAVLNEMRQGGVEPDVLTFNELINICVEGGDMKAAAGLMQAMVHRGMAPDTRTYTTLIKGHGLKGNLMDAFETVKTMRAQALPLSTYSCNVLADACIRCGQPSR 314
            N  I+   K+   ++   +  +++ +G+  +  TF+ L++     +R +  T  + Q + E  GL+P  +TYN +L  +  +G     ++A  +L  M   G E DV+T+  LIN   + G  + A  L++ M  +G+ P  + Y  +I+    + NL DA    + M     P    +  ++  +  R G P +
Sbjct:  481 NTIIDALCKKMRIEEAEEVFDQMDAQGISRSAVTFNTLIDGLCKAKRIDDATELIEQMVKE--GLQPNNITYNSILTHYCKQGNI---KKAADILETMTANGFEIDVVTYGTLINGLCKAGRTQVALKLLRGMRIKGIRPTPKAYNPVIQSLFRRNNLRDALNLFREMTEVGEPPDALTYKIVFRSLCRGGGPIK 670