EWM29648.1, cds1034 (CDS) Nannochloropsis gaditana

BLAST of EWM29648.1 vs. NCBI_GenBank
Match: gi|585112220|gb|EWM29648.1| (phosphoglycerate mutase 1 family [Nannochloropsis gaditana])

HSP 1 Score: 1180.24 bits (3052), Expect = 0.000e+0
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query:    1 MLTMRRLLNTQPRKTLLHKLFFRVAIFGAIISFRPASAFLSPLVPRCSFTFRHEVSSTIVCMMAPHRVHGVDSMWNSLHHGSNTTFVSEIEDDVFAGSNSWAKKVYRALNAATKRKEPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPIEQEGAVSPLTGEFLEKKGLLRAALAKEAELAASVPGYSTDGGGFRGNANLPVMDARLRGLAKLSEARQLIAATSLGVPPPLAPLPQAVPATRVAYPRLVVPKAASSPPSAGAPDAKNAQEHDQIIVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNEVGREGCQGGSMRGWGSSMCDVSD 571
            MLTMRRLLNTQPRKTLLHKLFFRVAIFGAIISFRPASAFLSPLVPRCSFTFRHEVSSTIVCMMAPHRVHGVDSMWNSLHHGSNTTFVSEIEDDVFAGSNSWAKKVYRALNAATKRKEPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPIEQEGAVSPLTGEFLEKKGLLRAALAKEAELAASVPGYSTDGGGFRGNANLPVMDARLRGLAKLSEARQLIAATSLGVPPPLAPLPQAVPATRVAYPRLVVPKAASSPPSAGAPDAKNAQEHDQIIVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNEVGREGCQGGSMRGWGSSMCDVSD
Sbjct:    1 MLTMRRLLNTQPRKTLLHKLFFRVAIFGAIISFRPASAFLSPLVPRCSFTFRHEVSSTIVCMMAPHRVHGVDSMWNSLHHGSNTTFVSEIEDDVFAGSNSWAKKVYRALNAATKRKEPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPIEQEGAVSPLTGEFLEKKGLLRAALAKEAELAASVPGYSTDGGGFRGNANLPVMDARLRGLAKLSEARQLIAATSLGVPPPLAPLPQAVPATRVAYPRLVVPKAASSPPSAGAPDAKNAQEHDQIIVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNEVGREGCQGGSMRGWGSSMCDVSD 571          

BLAST of EWM29648.1 vs. NCBI_GenBank
Match: gi|1072222249|gb|OEU07017.1| (hypothetical protein FRACYDRAFT_252624 [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 412.149 bits (1058), Expect = 1.191e-130
Identity = 234/520 (45.00%), Postives = 308/520 (59.23%), Query Frame = 0

Query:  103 KKVYRALNAATKRK--EPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEID--VTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMT--PDHPHWHMH----EKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKN-----MVPI-EQEGAVSPLT------GEFLEKKGLLRAALAKEAELAASVPGYSTDGGGFRGNANLPVMDARL-------RGLAKLSEARQLIA----------------------------------ATSLGVPPPLAPLPQAVPATR---------VAYPRLVVPKAASSPPSAGAPDAKNAQ--EHDQIIVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNE 548
            KK YR L     +   EPG LI++RHGES+ N NKTFTGW D DL  +G RE EH+ARLLME GY  D  V +TSRLKRAIRS+WIL+ E  ++Y PV+KSWRLNER YGAL G+SK   AE LG E+VQ +R  LT  PPP+    D  H+  +    ++KYAD+  ++IP+TESL+D M RT PLW+ +I+ +L+ GR V++VAHAN+LRG+VK ID++   EIQ+V IP GIP++YKF K+     ++ I + +G  S L       G+FLEK GLL+ AL  E    A VPGY             P+M  ++       R L KL+  R+L A                                   T+      +AP   A+ A           ++       +  ++     +    N Q    D +IVI+RHGKT++NKLG+FTGWEDAPLA EG  EA+ AG+ LK+ G E DVVYTSWLSRA+ETAW ++DE+DCLW+PIVK+WRLNE
Sbjct:  134 KKPYRKLKKLITKDTTEPGTLILVRHGESIWNKNKTFTGWADPDLTIQGYREVEHAARLLMEGGYHYDIHVVFTSRLKRAIRSTWILLQEFNEVYLPVFKSWRLNERNYGALTGLSKTQTAERLGAELVQGWRGSLTKKPPPLDIKNDPIHYQQYYPGNDRKYADLTEDQIPLTESLKDCMIRTKPLWDDKIVYELKKGRNVLVVAHANTLRGLVKTIDDIGDEEIQNVQIPTGIPIIYKFKKDVDTGQLISIDDDDGGNSNLVSQVHMKGKFLEKPGLLKEALKLEEGWKARVPGYD------------PIMQRQIRPMTGLERSLYKLNAVRELGAWANEFIDLDMLKNNGDGKVGEDTDSNDDGNGIFFTTNGNGNNKIAPTTTAIVANNGRGDVGADIISDQEEDDVRGTNNDDEVSSSGGSNTQTKRQDSVIVIIRHGKTQHNKLGLFTGWEDAPLAEEGIEEAKLAGRNLKKFGFEFDVVYTSWLSRALETAWFVMDEMDCLWLPIVKTWRLNE 641          

HSP 2 Score: 173.711 bits (439), Expect = 1.788e-42
Identity = 103/278 (37.05%), Postives = 155/278 (55.76%), Query Frame = 0

Query:   91 EDDVFAGSNSWAKKVYRALNAATKRKEPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPM---TPDHPHWHMHEKKYA-DV---------------KP---EEIPVTESLQDTMERTLPLWESRILPD-LRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPIEQEGA 345
            EDDV   +N          N  TKR++   +++IRHG++  N    FTGW DA L E GI E + + R L + G+E DV YTS L RA+ ++W +M E+  ++ P+ K+WRLNERMYG L G+SK  + +  GE+  +A+R G    PPP+   + D+P      +KY  DV               KP    ++P TESL+D M+RTLP +  RI  D +   + V+I +  N++RG++ H+ ++ Q EI  + IPNG+PL+Y      V +  +G 
Sbjct:  529 EDDVRGTNNDDEVSSSGGSNTQTKRQD-SVIVIIRHGKTQHNKLGLFTGWEDAPLAEEGIEEAKLAGRNLKKFGFEFDVVYTSWLSRALETAWFVMDEMDCLWLPIVKTWRLNERMYGNLTGLSKQMVKQRHGEKQFKAWRRGFDVRPPPVSSFSKDYPGNDPRYQKYLRDVRYSIKESCIRSIEKGKPVLVRKLPKTESLKDCMDRTLPYFIERIEKDAIDQNKRVLISSSENAIRGLLMHLCDIPQDEITGLEIPNGLPLIYDIRSKCVKLLDDGT 805          

BLAST of EWM29648.1 vs. NCBI_GenBank
Match: gi|219110028|ref|XP_002176766.1| (phosphoglycerate mutase [Phaeodactylum tricornutum CCAP 1055/1] >gi|217411301|gb|EEC51229.1| phosphoglycerate mutase [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 327.02 bits (837), Expect = 5.052e-98
Identity = 158/276 (57.25%), Postives = 204/276 (73.91%), Query Frame = 0

Query:  108 ALNAATKRKEPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPIEQEGAVSP-----LTGEFLEKKGLLRAALAKEAELAASVPGYS 378
            A N   K  +PG LI+IRHG+S+ N NKTFTGW D DL+E G+RE EH+ARLL+E GYEIDV +TSRLKRAIRS WI++ EL ++Y PV+KSWRLNERMYGAL G+SK   AE+LG  +VQ +R  L + PP +TP HP W   ++KYAD+  ++IP+TESL D MERT P+WE +I+ +LR GR VM+VAHAN+LRG+VK ID ++  +I+ VAIP GIP++YKFD+NM  I  EG         + G FLE+ GLL+ ALA+E E +  VPGY+
Sbjct:  163 AKNVLWKPPQPGTLILIRHGQSVWNSNKTFTGWADPDLSELGLREVEHAARLLLEGGYEIDVVFTSRLKRAIRSVWIILQELEEVYLPVFKSWRLNERMYGALTGLSKKETAEQLGSSIVQEWRGSLKSRPPALTPTHPFWPGKDRKYADLSSDQIPMTESLMDCMERTAPVWEDKIMYELRKGRNVMVVAHANTLRGLVKTIDGVSDEDIREVAIPTGIPIIYKFDENMKSIPPEGDRQTKSQIHMKGLFLEQPGLLKEALAREEEWSKQVPGYN 438          

HSP 2 Score: 157.532 bits (397), Expect = 4.687e-37
Identity = 82/248 (33.06%), Postives = 139/248 (56.05%), Query Frame = 0

Query:  121 LIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYA----DVK------------------PEEIPVTESLQDTMERTLPLWESRILPD-LRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPIEQEGA 345
            +++IRHG++  N    FTGW DA L   G+ E   + RLL   G+E DV YTS L RAI ++W ++ E+  ++ P+ K+WRLNERMYG L G+SK  + +  G    + +R G    PPP++    H+  ++ +Y     D++                    ++P +ESL++ M+RT+P +  RI+P+ +  G+ V+I +  N++RG++ H+  + + +I  + IPNG+PLV+      V +  +G 
Sbjct:  572 IVIIRHGKTEHNKLGLFTGWEDAPLAREGVDEAREAGRLLKAHGFEFDVVYTSWLSRAIETAWHVIDEMDALWLPIVKTWRLNERMYGKLTGLSKQMVRQRHGPTQFKKWRRGYKDPPPPVSSFSQHYPGNDPRYEKYLNDIRYSASESIIRSIEKGRPTLHRKLPKSESLKNCMDRTIPFFTERIVPEAIDQGKRVLISSSENAIRGLLMHLCEIPEDKITELEIPNGLPLVFDLRSKCVKLLDDGT 819          

HSP 3 Score: 149.828 bits (377), Expect = 1.887e-34
Identity = 67/87 (77.01%), Postives = 77/87 (88.51%), Query Frame = 0

Query:  462 DQIIVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNE 548
            D +IVI+RHGKTE+NKLG+FTGWEDAPLA EG  EAR AG+LLK HG E DVVYTSWLSRAIETAW ++DE+D LW+PIVK+WRLNE
Sbjct:  569 DAVIVIIRHGKTEHNKLGLFTGWEDAPLAREGVDEAREAGRLLKAHGFEFDVVYTSWLSRAIETAWHVIDEMDALWLPIVKTWRLNE 655          

HSP 4 Score: 85.1149 bits (209), Expect = 1.432e-13
Identity = 38/84 (45.24%), Postives = 58/84 (69.05%), Query Frame = 0

Query:  465 IVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNE 548
            ++++RHG++ +N    FTGW D  L+  G  E  +A +LL   G E+DVV+TS L RAI + W+I+ EL+ +++P+ KSWRLNE
Sbjct:  176 LILIRHGQSVWNSNKTFTGWADPDLSELGLREVEHAARLLLEGGYEIDVVFTSRLKRAIRSVWIILQELEEVYLPVFKSWRLNE 259          

BLAST of EWM29648.1 vs. NCBI_GenBank
Match: gi|1210512636|dbj|GAX28226.1| (hypothetical protein FisN_27Hh126 [Fistulifera solaris])

HSP 1 Score: 323.553 bits (828), Expect = 1.632e-97
Identity = 169/344 (49.13%), Postives = 226/344 (65.70%), Query Frame = 0

Query:   91 EDDVFAGSNSWAKKVYRALNAATK------RKEPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPI---EQEGAVSP--LTGEFLEKKGLLRAALAKEAELAASVPGYSTDGGGFRGNANLPVMDARLRGLAKLSEARQLIAATSLGVPPPLAP 423
            +DD +    S + K+   L +         R EPG LI++RHGES+ N N TFTGW D  L+ERG RE EH+ARLL+E GYEID+ +TSRLKRAI SSWI++ E+ QIY+PV+K+WRLNERMYGAL G+ K   AE LG ++VQ +R  L T PPP+TP+  +W   ++KYAD+  E+IP+TESL D MERT P+WE +I  +LR GR VM+V HAN+LRG+VK ID ++  +IQ V+IP GIP++YKFD+++ PI    ++  VS   + G FLEK GLL+ AL +E E A  VPGY+          ++P+     R L KL   R+L       + P  AP
Sbjct:  120 DDDGYNSMRSESSKMITVLKSINNVIFPKPRVEPGTLILVRHGESLWNANATFTGWADPGLSERGRREVEHAARLLLEGGYEIDIVFTSRLKRAIMSSWIMLQEMEQIYKPVFKTWRLNERMYGALTGLCKFETAERLGADLVQEWRGSLRTRPPPLTPNDEYWPGRDRKYADLSLEQIPMTESLLDCMERTAPVWEDKIKYELRHGRNVMVVGHANTLRGLVKTIDGISDEDIQMVSIPTGIPIIYKFDRDLKPIPPSAEKSTVSQVHMKGLFLEKPGLLKEALKREEEWANQVPGYNNTLALSSSQMSVPLTSLE-RSLLKLRAERELGEWAGQFIDPNAAP 462          

HSP 2 Score: 161.77 bits (408), Expect = 1.435e-38
Identity = 83/240 (34.58%), Postives = 145/240 (60.42%), Query Frame = 0

Query:  115 RKEPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKY----ADVK------------------PEEIPVTESLQDTMERTLPLWESRILPD-LRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVY 331
            R++P  +++IRHG++  N    FTGW D  L + GI E + + RLL + G+E DV YTS L RAI ++W +++E+  ++ P+ K+WRLNERMYG L G+SK  + +  GE   +A+R G  T PP ++   PH+  ++ +Y    +D++                    ++P +ESL+D M+RT+P +   I+P  +  G+ V+I +  N++RG++ H+ ++ + +I  + IPNG+P+++
Sbjct:  553 RRDP-VIVIIRHGKTGHNLLGLFTGWDDVALAQDGIEEAKDAGRLLKKYGFEFDVVYTSWLSRAIETAWHVLSEMDCLWLPIIKTWRLNERMYGELTGLSKQMVKQRHGETQFKAWRRGYKTPPPKVSSFSPHYPGNDPRYSKYLSDLRYSVRESLIRSIEKGRPTLARKLPKSESLKDCMDRTIPFFTECIVPQAVDQGKRVLISSSENAIRGLLMHLCDIPEDKIVELEIPNGLPIIF 791          

HSP 3 Score: 137.502 bits (345), Expect = 1.739e-30
Identity = 63/109 (57.80%), Postives = 80/109 (73.39%), Query Frame = 0

Query:  441 PKAASSPPSAGAPD-AKNAQEHDQIIVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNE 548
            P   S P     P   K     D +IVI+RHGKT +N LG+FTGW+D  LA +G  EA++AG+LLK++G E DVVYTSWLSRAIETAW ++ E+DCLW+PI+K+WRLNE
Sbjct:  533 PCVTSVPSQVLVPGMGKTPVRRDPVIVIIRHGKTGHNLLGLFTGWDDVALAQDGIEEAKDAGRLLKKYGFEFDVVYTSWLSRAIETAWHVLSEMDCLWLPIIKTWRLNE 641          

HSP 4 Score: 84.7297 bits (208), Expect = 1.717e-13
Identity = 35/84 (41.67%), Postives = 59/84 (70.24%), Query Frame = 0

Query:  465 IVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNE 548
            ++++RHG++ +N    FTGW D  L+  GR E  +A +LL   G E+D+V+TS L RAI ++W+++ E++ ++ P+ K+WRLNE
Sbjct:  156 LILVRHGESLWNANATFTGWADPGLSERGRREVEHAARLLLEGGYEIDIVFTSRLKRAIMSSWIMLQEMEQIYKPVFKTWRLNE 239          

BLAST of EWM29648.1 vs. NCBI_GenBank
Match: gi|1210517801|dbj|GAX23348.1| (hypothetical protein FisN_27Lh126 [Fistulifera solaris])

HSP 1 Score: 322.398 bits (825), Expect = 1.085e-96
Identity = 169/344 (49.13%), Postives = 225/344 (65.41%), Query Frame = 0

Query:   91 EDDVFAGSNSWAKKVYRALNAATK------RKEPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPI---EQEGAVSP--LTGEFLEKKGLLRAALAKEAELAASVPGYSTDGGGFRGNANLPVMDARLRGLAKLSEARQLIAATSLGVPPPLAP 423
            +DD +    S + K+   L +         R EPG LI++RHGES+ N N TFTGW D  L+ERG RE EH+ARLL+E GYEID+ +TSRLKRAI SSWI++ E+ QIY+PV+K+WRLNERMYGAL G+ K   AE LG ++VQ +R  L T PPP+TP+   W   ++KYAD+  E+IP+TESL D MERT P+WE +I  +LR GR VM+V HAN+LRG+VK ID ++  +IQ V+IP GIP++YKFD+++ PI    ++  VS   + G FLEK GLL+ AL +E E A  VPGY+          ++P+     R L KL   R+L       + P  AP
Sbjct:   93 DDDGYNSMRSESSKMISVLKSINNVLFPKPRVEPGTLILVRHGESLWNANATFTGWADPGLSERGRREVEHAARLLLEGGYEIDIVFTSRLKRAIMSSWIMLQEMEQIYKPVFKTWRLNERMYGALTGLCKFETAERLGADLVQEWRGSLRTRPPPLTPNDEFWPGRDRKYADLSLEQIPMTESLLDCMERTAPVWEDKIKYELRHGRNVMVVGHANTLRGLVKTIDGISDEDIQMVSIPTGIPIIYKFDRDLKPIPPSAEKSTVSQVHMKGLFLEKPGLLKEALKREEEWANQVPGYNNTLALSSSQMSVPLTSLE-RSLLKLRAERELGEWAGQFIDPNAAP 435          

HSP 2 Score: 164.851 bits (416), Expect = 1.880e-39
Identity = 85/254 (33.46%), Postives = 149/254 (58.66%), Query Frame = 0

Query:  115 RKEPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKY----ADVK------------------PEEIPVTESLQDTMERTLPLWESRILPD-LRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPIEQEGA 345
            R++P  +++IRHG++  N    FTGW D  L + GI E + + RLL   G+E DV YTS L RAI ++W +++E+  ++ P+ K+WRLNERMYG L G+SK  + +  GE   +A+R G  T PP ++   PH+  ++ +Y    +D++                    ++P +ESL+D M+RT+P +   I+P  +  G+ V+I +  N++RG++ H+ ++ + +I  + IPNG+P+++      V +  +G+
Sbjct:  525 RRDP-VIVIIRHGKTGHNLLGLFTGWDDVALAQDGIEEAKEAGRLLKRYGFEFDVVYTSWLSRAIETAWHVLSEMDCLWLPIIKTWRLNERMYGELTGLSKQMVKQRHGETQFKAWRRGYKTPPPKVSSFSPHYPGNDPRYSKYLSDLRYSVRESLIRSIEKGRPTLARKLPKSESLKDCMDRTIPFFTECIVPQAVDQGKRVLISSSENAIRGLLMHLCDIPEDKIVELEIPNGLPIIFDVKSKCVKLLDDGS 777          

HSP 3 Score: 137.502 bits (345), Expect = 1.833e-30
Identity = 64/109 (58.72%), Postives = 79/109 (72.48%), Query Frame = 0

Query:  441 PKAASSPPSAGAPD-AKNAQEHDQIIVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNE 548
            P   S P     P   K     D +IVI+RHGKT +N LG+FTGW+D  LA +G  EA+ AG+LLKR+G E DVVYTSWLSRAIETAW ++ E+DCLW+PI+K+WRLNE
Sbjct:  505 PCVTSVPSQVLVPGMGKTPVRRDPVIVIIRHGKTGHNLLGLFTGWDDVALAQDGIEEAKEAGRLLKRYGFEFDVVYTSWLSRAIETAWHVLSEMDCLWLPIIKTWRLNE 613          

HSP 4 Score: 84.3445 bits (207), Expect = 2.066e-13
Identity = 35/84 (41.67%), Postives = 59/84 (70.24%), Query Frame = 0

Query:  465 IVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNE 548
            ++++RHG++ +N    FTGW D  L+  GR E  +A +LL   G E+D+V+TS L RAI ++W+++ E++ ++ P+ K+WRLNE
Sbjct:  129 LILVRHGESLWNANATFTGWADPGLSERGRREVEHAARLLLEGGYEIDIVFTSRLKRAIMSSWIMLQEMEQIYKPVFKTWRLNE 212          

BLAST of EWM29648.1 vs. NCBI_GenBank
Match: gi|922855622|gb|KOO24055.1| (phosphoglycerate mutase [Chrysochromulina sp. CCMP291])

HSP 1 Score: 316.62 bits (810), Expect = 7.802e-96
Identity = 191/468 (40.81%), Postives = 260/468 (55.56%), Query Frame = 0

Query:   99 NSWAKKVYRALNAATKRKEPGKLIMIRHGESMLNYN--KTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPM---TPDHPHWHMHEKKYAD----VKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPIEQEGAVSPLTGEFLE--KKGLLRAALAKEAELAASVPGYSTDGGGFRGNANLPVMDARLRGLAKLSEARQLIAATSL--GVPPPLAPLPQAVPATRVAYPRLVVPKAASSPPSAGA-----PDAKNAQEHDQIIVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNE 548
            NSW  +    L        PG LI++RHG S       + F GW D DL E G ++   +AR + E GY  D+ YTS LKRA+R+ W+L+ EL  I+   +K+WRLNER YGAL G +   +  + G + V  +R   T  PPP    +P +P      K++ D    ++P  +P  ES+QDT+ R LP+W+  ILPDLR G++V++VAHANS+R +V+ ID +   E++ + IP  IPLVY+FD+   P E     +     +L+  ++   R AL + A L   V  +S            P+    L   +KL+ A++ + A SL                A   + P L      S   S G      P  K  Q   Q ++I+RHGKTE+NKLG+FTGWED  LA EGR EA  AGKLL+ HGIE DVVYTSWL RAIETAWL++ ELD +W+PI KSWRLNE
Sbjct:   51 NSWGTRFTNFLENPAP--PPGTLILLRHGASEAEQREPRVFVGWSDPDLCELGEQQAIEAARAIKEAGYTFDIVYTSMLKRAVRTVWLLLMELECIHVACWKTWRLNERCYGALTGRTLQEMQAKYGADTVAGWRRAFTARPPPFDPASPLNPDRDPRYKRWYDRRGVMRPVAVPDGESMQDTIARCLPVWKQEILPDLRRGKSVLVVAHANSIRALVQTIDGVTNDELRDLEIPVAIPLVYRFDRKN-PFEDLADQASWAPSWLQLRRRRTARQALQQLANLVPIVSDHSAS----------PLSGEYLAKPSKLAAAQESVRAASLRRHDEASATAAASDTNAHDFSAPPLTDTHVLSGGSSGGVWVRPRPPRKRDQ---QYVIIIRHGKTEHNKLGLFTGWEDVELAEEGRQEAIRAGKLLRAHGIEFDVVYTSWLQRAIETAWLVLVELDAMWLPIHKSWRLNE 502          

HSP 2 Score: 154.836 bits (390), Expect = 2.248e-36
Identity = 84/243 (34.57%), Postives = 138/243 (56.79%), Query Frame = 0

Query:  114 KRKEPGKLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYA----DVK------------------PEEIPVTESLQDTMERTLPLWESRILPD-LRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKF 333
            ++++   +I+IRHG++  N    FTGW D +L E G +E   + +LL   G E DV YTS L+RAI ++W+++ EL  ++ P++KSWRLNERMYGAL G+SK       GE+  + +R G    PP ++    H+  ++++Y     DV+                    ++P +ESL+D MERT+P + + I  + +  G+ V+I +  N++RG++ H+  +    I  + IP G+PLVY  
Sbjct:  412 RKRDQQYVIIIRHGKTEHNKLGLFTGWEDVELAEEGRQEAIRAGKLLRAHGIEFDVVYTSWLQRAIETAWLVLVELDAMWLPIHKSWRLNERMYGALTGLSKKMTRTIYGEKQFKKWRRGYDVKPPKVSSFSRHYPGNDQRYVENMLDVRLSFKESVIRSLERGSLSLHRKLPHSESLKDCMERTIPYFVNNIEKNAIARGKNVLIASSENAIRGLLMHLLEIPTDRIVDIEIPTGLPLVYDL 654          

BLAST of EWM29648.1 vs. NCBI_GenBank
Match: gi|676393001|ref|XP_009039778.1| (hypothetical protein AURANDRAFT_5727, partial [Aureococcus anophagefferens] >gi|323449764|gb|EGB05650.1| hypothetical protein AURANDRAFT_5727, partial [Aureococcus anophagefferens])

HSP 1 Score: 289.656 bits (740), Expect = 1.295e-91
Identity = 140/216 (64.81%), Postives = 167/216 (77.31%), Query Frame = 0

Query:  121 LIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMH--EKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFD 334
            LI++RHGES  N N TFTGW D DL++RG RE EH+ARLL+E GY +DV YTSRL+RAIRS WIL+  LG++Y PV+KSWRLNER YGAL G+SKPGLA ELGE  VQAFR GL   PPPM  D  H +    ++KYAD+    +P TESL DTM R LPLW+S+I PDL +GR VM+VAH NSLRG+VKHID ++  EI SV+IPNGIPLVYKF+
Sbjct:    1 LILVRHGESTWNANSTFTGWADVDLSDRGEREVEHAARLLLEAGYVVDVAYTSRLRRAIRSCWILLRGLGKVYSPVFKSWRLNERHYGALTGLSKPGLALELGEAAVQAFRHGLEDLPPPMPADGSHAYDDRGDRKYADLG--TVPATESLSDTMARALPLWDSKIKPDLLAGRNVMVVAHGNSLRGLVKHIDGISDDEITSVSIPNGIPLVYKFE 214          

HSP 2 Score: 78.5666 bits (192), Expect = 5.832e-13
Identity = 35/84 (41.67%), Postives = 54/84 (64.29%), Query Frame = 0

Query:  465 IVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNE 548
            ++++RHG++ +N    FTGW D  L++ G  E  +A +LL   G  +DV YTS L RAI + W+++  L  ++ P+ KSWRLNE
Sbjct:    1 LILVRHGESTWNANSTFTGWADVDLSDRGEREVEHAARLLLEAGYVVDVAYTSRLRRAIRSCWILLRGLGKVYSPVFKSWRLNE 84          

BLAST of EWM29648.1 vs. NCBI_GenBank
Match: gi|676394263|ref|XP_009040409.1| (hypothetical protein AURANDRAFT_14255, partial [Aureococcus anophagefferens] >gi|323448960|gb|EGB04852.1| hypothetical protein AURANDRAFT_14255, partial [Aureococcus anophagefferens])

HSP 1 Score: 257.299 bits (656), Expect = 4.045e-79
Identity = 122/217 (56.22%), Postives = 157/217 (72.35%), Query Frame = 0

Query:  120 KLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKN 336
            KL+++RHGES  N NKTF+GW+D DLN  G RE EH+ARL+MERG  IDV YTS LKRAIRS+WIL+ E  Q +RPV K WRLNER YGA +G SKPGLA  LG E V+ +R G    PP +   H      E+KY  V  +++P+TESL+D +ER LP++E RI  D+  G+ V++VAH N+LRG+VK ID L+   I++VAIPNGIPLVY+F ++
Sbjct:    1 KLVLVRHGESTWNANKTFSGWVDVDLNGVGEREVEHAARLMMERGLTIDVVYTSLLKRAIRSAWILLRESKQSFRPVVKDWRLNERHYGAFQGRSKPGLAALLGYEQVRDYRAGFVARPPALPRHHALSPFRERKYTGVAADDLPLTESLRDCLERALPVYEDRIKADVLDGKDVVVVAHGNALRGLVKAIDGLSDDVIENVAIPNGIPLVYEFARS 217          

HSP 2 Score: 80.4925 bits (197), Expect = 1.237e-13
Identity = 40/97 (41.24%), Postives = 58/97 (59.79%), Query Frame = 0

Query:  465 IVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNEVGREGCQGGSMRG 561
            +V++RHG++ +N    F+GW D  L   G  E  +A +L+   G+ +DVVYTS L RAI +AW+++ E    + P+VK WRLNE      QG S  G
Sbjct:    2 LVLVRHGESTWNANKTFSGWVDVDLNGVGEREVEHAARLMMERGLTIDVVYTSLLKRAIRSAWILLRESKQSFRPVVKDWRLNERHYGAFQGRSKPG 98          

BLAST of EWM29648.1 vs. NCBI_GenBank
Match: gi|676398747|ref|XP_009042651.1| (hypothetical protein AURANDRAFT_6558, partial [Aureococcus anophagefferens] >gi|323446519|gb|EGB02652.1| hypothetical protein AURANDRAFT_6558, partial [Aureococcus anophagefferens])

HSP 1 Score: 253.832 bits (647), Expect = 8.086e-78
Identity = 121/213 (56.81%), Postives = 154/213 (72.30%), Query Frame = 0

Query:  120 KLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYK 332
            KL+++RHGES  N NKTF+GW+D DLN  G RE EH+ARL+MERG  IDV YTS LKRAIRS+WIL+ E  Q +RPV K WRLNER YGA +G SKPGLA  LG E V+ +R G    PP +   H      E+KY  V  +++P+TESL+D +ER LP++E RI  D+  G+ V++VAH N+LRG+VK ID L+   I++VAIPNGIPLVY+
Sbjct:    1 KLVLVRHGESTWNANKTFSGWVDVDLNGVGEREVEHAARLMMERGLTIDVVYTSLLKRAIRSAWILLRESKQSFRPVVKDWRLNERHYGAFQGRSKPGLAALLGYEQVRDYRAGFVARPPALPRHHALSPFRERKYTGVAADDLPLTESLRDCLERALPVYEDRIKADVLDGKDVVVVAHGNALRGLVKAIDGLSDDVIENVAIPNGIPLVYE 213          

HSP 2 Score: 80.4925 bits (197), Expect = 1.421e-13
Identity = 40/97 (41.24%), Postives = 58/97 (59.79%), Query Frame = 0

Query:  465 IVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNEVGREGCQGGSMRG 561
            +V++RHG++ +N    F+GW D  L   G  E  +A +L+   G+ +DVVYTS L RAI +AW+++ E    + P+VK WRLNE      QG S  G
Sbjct:    2 LVLVRHGESTWNANKTFSGWVDVDLNGVGEREVEHAARLMMERGLTIDVVYTSLLKRAIRSAWILLRESKQSFRPVVKDWRLNERHYGAFQGRSKPG 98          

BLAST of EWM29648.1 vs. NCBI_GenBank
Match: gi|550170697|ref|WP_022587279.1| (phosphoglyceromutase [Caldanaerobacter subterraneus] >gi|548794696|gb|ERM92922.1| phosphoglyceromutase [Caldanaerobacter subterraneus subsp. yonseiensis KB-1] >gi|973029371|gb|KUK08245.1| 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase [Caldanaerobacter subterraneus])

HSP 1 Score: 241.506 bits (615), Expect = 1.504e-72
Identity = 110/221 (49.77%), Postives = 159/221 (71.95%), Query Frame = 0

Query:  120 KLIMIRHGESMLNYNKTFTGWIDADLNERGIRETEHSARLLMERGYEIDVTYTSRLKRAIRSSWILMTELGQIYRPVYKSWRLNERMYGALEGMSKPGLAEELGEEVVQAFRTGLTTTPPPMTPDHPHWHMHEKKYADVKPEEIPVTESLQDTMERTLPLWESRILPDLRSGRTVMIVAHANSLRGIVKHIDNLNQTEIQSVAIPNGIPLVYKFDKNMVPI 340
            K++++RHGES+ N    FTGW D DL+ +GI E + + ++L E+G+  DV +TS LKRAIR+ WI++ EL  ++ PVYKSWRLNER YGAL+G++K   A++ GEE V+ +R      PP +T D P +  ++ +YAD+  +EIP+TE+L DT+ R +P WES I P ++SG+ V+I AH NSLRG+VK++DNL+  EI  + IP GIPLVY+ D+N+ PI
Sbjct:    3 KVVLLRHGESIWNMENRFTGWTDVDLSPKGIEEAKQAGKILKEKGFTFDVAFTSVLKRAIRTLWIVLDELDLMWIPVYKSWRLNERHYGALQGLNKAETAKKYGEEQVKLWRRSAEVRPPALTKDDPRYPGNDPRYADLSEDEIPLTENLIDTINRVIPYWESTIAPTIKSGKRVIIAAHGNSLRGLVKYLDNLSNEEIMELNIPTGIPLVYELDENLKPI 223          

HSP 2 Score: 100.523 bits (249), Expect = 3.325e-20
Identity = 45/92 (48.91%), Postives = 62/92 (67.39%), Query Frame = 0

Query:  465 IVIMRHGKTEYNKLGVFTGWEDAPLANEGRIEARNAGKLLKRHGIELDVVYTSWLSRAIETAWLIVDELDCLWVPIVKSWRLNEVGREGCQG 556
            +V++RHG++ +N    FTGW D  L+ +G  EA+ AGK+LK  G   DV +TS L RAI T W+++DELD +W+P+ KSWRLNE      QG
Sbjct:    4 VVLLRHGESIWNMENRFTGWTDVDLSPKGIEEAKQAGKILKEKGFTFDVAFTSVLKRAIRTLWIVLDELDLMWIPVYKSWRLNERHYGALQG 95