EWM29670.1, cds1005 (CDS) Nannochloropsis gaditana

Overview
NameEWM29670.1
Unique Namecds1005
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002456.1:945905..946819 +

Link to JBrowse

Properties
Property NameValue
Protein idEWM29670.1
ProductKua-ubiquitin conjugating enzyme hybrid, localization
Orig transcript idgnl|cribi|Naga_100112g14.5453.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002456.1supercontigCM002456.1:945905..946819 +
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR019547Kua
Homology
BLAST of EWM29670.1 vs. NCBI_GenBank
Match: gi|585112244|gb|EWM29670.1| (Kua-ubiquitin conjugating enzyme hybrid, localization [Nannochloropsis gaditana])

HSP 1 Score: 626.706 bits (1615), Expect = 0.000e+0
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0
Query:    1 MCVSARSETATPPAAVSDTSASGSIKRYYDNAGLGRQDGDAIDQLNAFMTKQAHKVWSPDNEDYKVYEMVRQGHVVFKRRCMPLLYFYWATILVGLLRAPSLLFLAGALASTYFYMELYSAVLHIVLDNPAFIKVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAINLLLFDWMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHRTYDRGFAILGGWSEGLITTLYHHVVPSQYVWLALFAVMTVFGLAGVVKIYIPAYDAFVAFVQ 304
            MCVSARSETATPPAAVSDTSASGSIKRYYDNAGLGRQDGDAIDQLNAFMTKQAHKVWSPDNEDYKVYEMVRQGHVVFKRRCMPLLYFYWATILVGLLRAPSLLFLAGALASTYFYMELYSAVLHIVLDNPAFIKVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAINLLLFDWMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHRTYDRGFAILGGWSEGLITTLYHHVVPSQYVWLALFAVMTVFGLAGVVKIYIPAYDAFVAFVQ
Sbjct:    1 MCVSARSETATPPAAVSDTSASGSIKRYYDNAGLGRQDGDAIDQLNAFMTKQAHKVWSPDNEDYKVYEMVRQGHVVFKRRCMPLLYFYWATILVGLLRAPSLLFLAGALASTYFYMELYSAVLHIVLDNPAFIKVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAINLLLFDWMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHRTYDRGFAILGGWSEGLITTLYHHVVPSQYVWLALFAVMTVFGLAGVVKIYIPAYDAFVAFVQ 304          
BLAST of EWM29670.1 vs. NCBI_GenBank
Match: gi|585105365|gb|EWM24073.1| (Kua-ubiquitin conjugating enzyme hybrid, localization [Nannochloropsis gaditana] >gi|585105366|gb|EWM24074.1| Kua-ubiquitin conjugating enzyme hybrid, localization [Nannochloropsis gaditana])

HSP 1 Score: 264.618 bits (675), Expect = 5.660e-84
Identity = 135/297 (45.45%), Postives = 182/297 (61.28%), Query Frame = 0
Query:    1 MCVSARSETATPPAAVSDTSAS--GSIKRYYDNAGLGRQDGDAIDQLNAFMTKQAHK-VWSPDNEDYKVYEMVRQGHVVFKRRCMPLLYFYWATILVGLLRAPSLLFLAGALASTYFYMELYSAVLHIVLDNPAFIKVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAINLLLFDWMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHRTYDRGFAILGGWSEGLITTLYHHVVPSQYVWLALFAVMTVFGLAGVVKIYIP 294
            MC+ + S    P  A S  + +   S+     +A  G    D +D+LN      A K +  P +E Y   +MV++G  VFK R  PL+Y YWA  + G+L  PSL F  GA   TY Y++LY AVLHIVLDNP F+K+P + +   EF  HHI+P EI  R +R I  DLN I+GL + +NL+LF+ + D    CV       AYLGQ AHRQAHM P K  P    LQ  GL+V+P  HR HH+TYD+GF IL GWS+  +T LY +VVPSQ+VWLA+F ++T  G+AG++++Y+P
Sbjct:    1 MCIGSVSAAIPPVTAASAEAVAQHSSMPVPESDAQDGDASMDIVDRLNVQAKHLAAKTIGVPGDEHYACLQMVKEGATVFKHRIWPLMYIYWAYTVYGILTGPSLAFALGAFVLTYLYIDLYGAVLHIVLDNPNFLKLPLIGEACLEFQFHHIIPHEITVRDFRHIAADLNGIIGLEYGVNLILFNGLTDPAYRCVACCAVLNAYLGQLAHRQAHMRPEKRDPVVAVLQGLGLMVTPDTHRRHHKTYDQGFPILSGWSDAPVTFLYRYVVPSQWVWLAMFVLLTFGGIAGLIRLYLP 297          
BLAST of EWM29670.1 vs. NCBI_GenBank
Match: gi|553187743|ref|XP_005854670.1| (kua-ubiquitin conjugating enzyme hybrid localization domain-containing protein [Nannochloropsis gaditana CCMP526] >gi|422294387|gb|EKU21687.1| kua-ubiquitin conjugating enzyme hybrid localization domain-containing protein [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 211.846 bits (538), Expect = 7.708e-63
Identity = 122/312 (39.10%), Postives = 176/312 (56.41%), Query Frame = 0
Query:    5 ARSETATPPAAVSDTSASGSIKRYYDNAGLGRQDGDA-------------IDQLNAFMTKQAH---KVWSPDNEDYKVYEMVRQGHVVFKRRCMPLLYFYWATILVGLLRAPSLLFLAGALASTYFYMELYSAVLHIVLDNPAFIKVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAINLLLF---DWMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHRTYDRGFAILGGWSEGLITTLYHHVVPSQYVWLALFAVMTVFGLAGVVKIYIPAYD 297
            + ++ AT   + + TS   S      +  +G  D ++             I++LN    K+A    KV +P + D+ +YE+VR+G+ VFK R M LLY YWA    GLLR PSL F   A   TY Y+ELY A+LH+ LDNP+F+ +P L +   EF  HH++P EI  R Y  I  DLN+IV L F +  ++F     + +  +   +A G   AYL ++AHRQAH P  K   FA+ LQD G LVS  LHR HH+T+D GF +L G SE +I  +   V  +Q+VWLA F +MT+ GL  ++ + +  ++
Sbjct:    2 STTKVATTTRSANCTSRPDSRLHDCSSTAVGGHDSESKARTWRILTPAQRIEKLNGMAMKRAQEMEKVLTPSHPDWPMYELVRRGNPVFKHRWMVLLYAYWALTGAGLLRCPSLAFGMSAFIITYLYIELYGAILHVNLDNPSFLFLPVLWEACLEFQYHHVIPSEITLRSYPQIAADLNLIVALQFIVCTIVFYAHGGLFNPYIHATMACGLVNAYLLEWAHRQAHFPRGKRHAFAQYLQDRGFLVSEALHRRHHQTFDVGFPVLSGLSEPVINFVRAKVCANQWVWLAAFILMTLAGLPCIISVNMRLWE 313          
BLAST of EWM29670.1 vs. NCBI_GenBank
Match: gi|585101427|gb|EWM21272.1| (Kua-ubiquitin conjugating enzyme hybrid, localization [Nannochloropsis gaditana])

HSP 1 Score: 211.846 bits (538), Expect = 7.876e-63
Identity = 120/312 (38.46%), Postives = 174/312 (55.77%), Query Frame = 0
Query:    5 ARSETATPPAAVSDTSASGSIKRYYDNAGLGRQDGDA-------------IDQLNAFMTKQAH---KVWSPDNEDYKVYEMVRQGHVVFKRRCMPLLYFYWATILVGLLRAPSLLFLAGALASTYFYMELYSAVLHIVLDNPAFIKVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAINLLLF---DWMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHRTYDRGFAILGGWSEGLITTLYHHVVPSQYVWLALFAVMTVFGLAGVVKIYIPAYD 297
            + ++ AT   + + TS   S    + +  +G  D ++             I++LN    K+A    KV +P + D+ +YE+VR+G+ VFK R M LLY YWA    GLLR PS  F   A   TY Y+ELY A+LH+ LDNP+F+ +P L +   EF  HH++P EI  R Y  I  DLN+IV + F +  ++F     + +  +   +A G   AYL ++AHRQAH P  K   FA+ LQD G LVS  LHR HH+T+D GF +L G SE +I  +   V  +Q+VWLA F  MT  GL  ++ + +  ++
Sbjct:    2 STTKVATTTQSANCTSRPDSRLHDFSSTAVGGHDSESKARTWRILTPAQRIEKLNGMAMKRAQEMEKVLTPSHPDWPMYELVRRGNPVFKHRWMVLLYAYWALTGTGLLRCPSFAFGMSAFIITYLYIELYGAILHVNLDNPSFLSLPVLWEACLEFQYHHVIPSEITLRSYPQIAADLNLIVAMQFIVCTIVFYAHGGLFNPYIHATMACGLVNAYLLEWAHRQAHFPRGKRHAFAQYLQDRGFLVSEALHRRHHQTFDVGFPVLSGLSEPVINFVRAKVCANQWVWLAAFISMTFAGLPCIISVNMRLWE 313          
BLAST of EWM29670.1 vs. NCBI_GenBank
Match: gi|676396509|ref|XP_009041532.1| (hypothetical protein AURANDRAFT_67743 [Aureococcus anophagefferens] >gi|323447839|gb|EGB03747.1| hypothetical protein AURANDRAFT_67743 [Aureococcus anophagefferens])

HSP 1 Score: 102.064 bits (253), Expect = 2.294e-21
Identity = 79/284 (27.82%), Postives = 129/284 (45.42%), Query Frame = 0
Query:    3 VSARSETATPPAAVSDTSASGSIKRYYD---NAGLGRQDGDAIDQLNAFMTKQ--AHKVWSPDNEDYKVYEMVRQGHVVFKRRCMPLLYFYWATILVGLLRAPSLLFLAGALASTYFYMELYSAVLHIVLD---NPAFIKVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAINLLLFDWMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHR-TYDRGFAILGGWSEGLITTLYHHVVPSQYVWLALF 277
            V+A+   A PPAA     A+   + Y D   N        D  D++   + +Q    K   P    + V      G +V+  R + +++ Y A  +  + +APS   +   + + Y   ++YS V H+ LD   N A +K   L Q   EF  HH +PV+I ++ +   C DLN+IV +   IN +   +   G    +       AY GQ+ HR AH P SK    A+ L D G +   + H  HH+  +D  F ++ G+ +G+I  +  H   + +VW   +
Sbjct:   24 VAAKLGRAAPPAARDPLLAAVPREVYMDAASNVPSASYYKDLNDKVADILERQRTGAKTAPPIELSHVV---AADGALVYPNRYLWMVWVYSAVAIYLICQAPSPAVVLLGVPAMYLCYDVYSGVTHVNLDDQRNLAGLKSYILFQGCLEFQWHHAIPVDITKKSFVACCADLNIIVAVSVVINCVALRYTT-GTAAALTGLKLFGAYFGQYCHRSAHTPKSKRPRVAQYLMDKGFMCPQNKHLGHHKPPHDANFCLV-GYCDGIINGMLKHCGTNDWVWFGFW 302          
BLAST of EWM29670.1 vs. NCBI_GenBank
Match: gi|298706641|emb|CBJ29579.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 100.908 bits (250), Expect = 2.388e-21
Identity = 78/225 (34.67%), Postives = 113/225 (50.22%), Query Frame = 0
Query:   68 EMVRQGHVVFKRRCMPLLYFYWATILVGLLR--------APSLLFLAGALASTYFYMELYSAVLHIVLDNPAFIKVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAINLLLFDWMKDGRVMCVVAAGGAM--AYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHR-TYDRGFAILGGWSEGLITTLYHHVVPSQYVWLALFAVMT 281
            + V  G + F +R   L+  Y    +  L+R        AP++    GA A  YF ++ YS VLH+VLD+   + VP L QP  EF  HH +P +   R + +  GDLN IVGL  A+   LF        M + A G  M  AY G + HRQAH   SK   +   LQ+ G+++SP  H+ HH   +D  + +LG  +   +T L    + S + W+ALF  +T
Sbjct:   38 QFVLGGRLPFVKRIETLVRGYVCVSMFFLIRYGILECGVAPTV----GAFAVMYFVLDFYSGVLHVVLDDDWNMNVPLLSQPAMEFQYHHHIPDDGCSRPFVEGMGDLNFIVGLHLALFTALFVRGGSQDFMLLTAGGWKMVLAYWGIWNHRQAHQLKSKRPRWCTYLQNNGIMLSPAAHKVHHTPPHDDEYCLLGV-TTWPVTWLGRRNLGSIF-WIALFLGLT 256          
BLAST of EWM29670.1 vs. NCBI_GenBank
Match: gi|954193073|gb|KRX05823.1| (hypothetical protein PPERSA_02355 [Pseudocohnilembus persalinus])

HSP 1 Score: 101.679 bits (252), Expect = 3.221e-21
Identity = 62/211 (29.38%), Postives = 102/211 (48.34%), Query Frame = 0
Query:   76 VFKRRCMPLLYFYWATILVGLLRAPSLLFLAGALASTYFYMELYSAVLHIVLDNPAFI---KVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAINLLLFDWMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHRTYDRGFAILGGWSEGLITTLYHHVVPSQYVWLALFAVMTVF 283
            V++ R   L+  Y    +  + ++PS      +    +F+ +L+S +LH+ LD+P  +   +   L +P  EF  HH++P +IA + Y    GDLN +VGLIFA    +   + +   M +       AY GQF+H+ +H      S F   LQ + +L+S   H+ HH  +D  F I  G   G+I +L   +  +  VW  +F     F
Sbjct:  101 VYEDRSKYLIAAYICLGIYLVSQSPSWQITVFSFFLNFFWYDLFSGILHVCLDDPRNLIGWRSAILYKPCLEFQWHHVIPADIASKPYYQSIGDLNFVVGLIFAFQCCM-GILSNPTAMVLSGFKFFFAYYGQFSHKMSHTFEGDRSIFVNLLQKSHILLSEKDHKVHHTNHDDNFCIGSGTFNGVIRSLL-KITTNPNVWFCIFGYSLFF 309          
BLAST of EWM29670.1 vs. NCBI_GenBank
Match: gi|676397009|ref|XP_009041782.1| (hypothetical protein AURANDRAFT_67953 [Aureococcus anophagefferens] >gi|323447600|gb|EGB03515.1| hypothetical protein AURANDRAFT_67953 [Aureococcus anophagefferens])

HSP 1 Score: 98.5969 bits (244), Expect = 3.921e-20
Identity = 82/305 (26.89%), Postives = 136/305 (44.59%), Query Frame = 0
Query:    3 VSARSETATPPAAVSDTSASGSIKRYYD---NAGLGRQDGDAIDQLNAFMTKQ--AHKVWSPDNEDYKVYEMVRQGHVVFKRRCMPLLYFYWATILVGLLRAPSLLFLAGALASTYFYMELYSAVLHIVLD---NPAFIKVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAINLLLFDWMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHR-TYDRGFAILGGWSEGLITTLYHHVVPSQYVWLAL---FAVMTVFGLAGVVKIYIPA 295
            V+A+   A PPAA     A+   + Y D   N        D  D++   + +Q    K   P    + V      G +V+  R + +++ Y A  +  + +APS   +   + + Y   ++YS V H+ LD   N A +K   L Q   EF  HH +PV+I ++ +   C DLN+IV +   IN +   +                AY GQ+ HR AH P SK    A+ L D G +   + H  HH+  +D  F ++ G+ +G+I  +  H   + +VW      F+   V+ +  V+ +  P+
Sbjct:   24 VAAKLGRAAPPAARDPLLAAVPREVYMDAASNVPSASYYKDLNDKVADILERQRTGAKTAPPIELSHVV---AADGALVYPNRYLWMVWVYSAVAIYLICQAPSPAVVLLGVPAMYLCYDVYSGVTHVNLDDQRNLAGLKSYILFQGCLEFQWHHAIPVDITKKSFVACCADLNIIVAVSVVINCVALRYTTGTAATLTGLKLFG-AYFGQYCHRSAHTPKSKRPRVAQYLMDKGFMCPQNKHLGHHKPPHDANFCLV-GYCDGIINGMLKHCGTNDWVWFGFWLAFSFGDVYVVNKVLTVAAPS 323          
BLAST of EWM29670.1 vs. NCBI_GenBank
Match: gi|1181277989|ref|WP_084583998.1| (carotenoid synthesis regulator CarF [Sphingomonas azotifigens])

HSP 1 Score: 68.1662 bits (165), Expect = 7.991e-10
Identity = 63/222 (28.38%), Postives = 96/222 (43.24%), Query Frame = 0
Query:   78 KRRCMPLLYFYWATILVGLLRAPSLLFLAGALASTYFYMELYSAVLHIVLD---NPA--------------------------FIKVPGLDQPVFEFLAHHILPVEIAQRRYRDICGDLNVIVGLIFAI--NLLLFD-----WMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHRTYDRGFAILGGWSEGLITTLY 263
            +R  MPL     A  LV +LR PS   +  AL + ++  +L S ++H+ +D    PA                            KV  LD+ +++F  HH  P  + +R    + G   ++  L F++  N+L F      W   G V  +V  GGA A     +  +AH P        RA++  GLL++P  H+ HH T  R FA   GWS  L+  L+
Sbjct:    3 RRILMPLFLLSLAANLVAVLRDPSWWVIP-ALLAGWYGADLASGLVHMYMDYKPTPAGKGLDALYFYAGSRESEHYKALWRERMAKVSPLDRLIYDFKNHHPRPDALGRRDLWRLIGSTVILAALPFSVMLNVLAFTGLVPGWAAAGGVTLIV--GGAFAQYFHGSLHRAHNPW-----IVRAMRRCGLLMTPAAHQLHHDTLQRDFATNCGWSNPLVNRLF 216          
BLAST of EWM29670.1 vs. NCBI_GenBank
Match: gi|976912646|gb|KVH99123.1| (Kua-ubiquitin conjugating enzyme hybrid, localization [Cynara cardunculus var. scolymus])

HSP 1 Score: 67.0106 bits (162), Expect = 2.547e-9
Identity = 51/183 (27.87%), Postives = 82/183 (44.81%), Query Frame = 0
Query:   74 HVVFKRRCMPLLYFYWATILVGLLRAPSLL--FLAGALASTYFYMELYSAVLHIVLDNPAFIKVPGLDQPVFEFLAHHILPVEIAQRRYR-DICGDLNVIVGLIFAINLLLFDWMKDGRVMCVVAAGGAMAYLGQFAHRQAHMPPSKVSPFARALQDAGLLVSPHLHRAHHRTYDRGFAILGG 253
            HV     C  +L+     ++ G   + + L   LAG +   Y +++L+S V H V DN     +P L   +  F  HH LP    +R++  ++     +I  ++  +NL+   W     VM  V             H  AH   SK+     ALQDAG++++   H AHHR+ D  + I+ G
Sbjct:   27 HVWVATGCTTVLFSLANAVVYGSTHSHTWLKTILAGFIG--YLFVDLFSGVYHWVFDNYGDFSIPILGSHIDTFRRHHELPWATTKRQFSSNLHVAARIITHVVPPMNLI---WHDQPAVMGFVGMFSGGVLFAVQIHAWAHESKSKLPAMVVALQDAGVVIAQSRHAAHHRSGDSSYCIVSG 204          
The following BLAST results are available for this feature:
BLAST of EWM29670.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585112244|gb|EWM29670.1|0.000e+0100.00Kua-ubiquitin conjugating enzyme hybrid, localizat... [more]
gi|585105365|gb|EWM24073.1|5.660e-8445.45Kua-ubiquitin conjugating enzyme hybrid, localizat... [more]
gi|553187743|ref|XP_005854670.1|7.708e-6339.10kua-ubiquitin conjugating enzyme hybrid localizati... [more]
gi|585101427|gb|EWM21272.1|7.876e-6338.46Kua-ubiquitin conjugating enzyme hybrid, localizat... [more]
gi|676396509|ref|XP_009041532.1|2.294e-2127.82hypothetical protein AURANDRAFT_67743 [Aureococcus... [more]
gi|298706641|emb|CBJ29579.1|2.388e-2134.67conserved unknown protein [Ectocarpus siliculosus][more]
gi|954193073|gb|KRX05823.1|3.221e-2129.38hypothetical protein PPERSA_02355 [Pseudocohnilemb... [more]
gi|676397009|ref|XP_009041782.1|3.921e-2026.89hypothetical protein AURANDRAFT_67953 [Aureococcus... [more]
gi|1181277989|ref|WP_084583998.1|7.991e-1028.38carotenoid synthesis regulator CarF [Sphingomonas ... [more]
gi|976912646|gb|KVH99123.1|2.547e-927.87Kua-ubiquitin conjugating enzyme hybrid, localizat... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna1006rna1006Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications