Antimicrobial activity of the Flo peptide produced in Scenedesmus acutus and Nannochloropsis oculata

Overview
TitleAntimicrobial activity of the Flo peptide produced in Scenedesmus acutus and Nannochloropsis oculata
AuthorsDávalos-Guzmán SD, Martinez-Gutierrez F, Martínez-González L, Quezada-Rivera JJ, Lorenzo-Leal AC, Bach H, Morales-Domínguez JF, Soria-Guerra RE
TypeJournal Article
Journal NameWorld journal of microbiology & biotechnology
Volume39
Issue8
Year2023
Page(s)211
CitationDávalos-Guzmán SD, Martinez-Gutierrez F, Martínez-González L, Quezada-Rivera JJ, Lorenzo-Leal AC, Bach H, Morales-Domínguez JF, Soria-Guerra RE. Antimicrobial activity of the Flo peptide produced in Scenedesmus acutus and Nannochloropsis oculata. World journal of microbiology & biotechnology. 2023 May 30; 39(8):211.

Abstract

The continuous increase of bacterial pathogen resistance to conventional antibiotics has challenged the research community to develop new antimicrobial strategies. Antimicrobial peptides (AMP) are a promising alternative to combat multidrug-resistant strains compared to conventional antibiotics because of their biocompatibility. In the present study, the Flo peptide, an AMP from the Moringa oleifera tree, was expressed in the chloroplast of the microalgae Nannochloropsis oculata and Scenedesmus acutus. The transgene insertion was verified by PCR amplification, and the homoplasmy was corroborated in spectinomycin-resistant lines. The identification and quantification of the peptide were performed using ELISA. The antimicrobial activity was studied against the Gram-negative Escherichia coli (ATCC 25,922) and Klebsiella pneumoniae (ATCC 700,603). The inflammatory response of the total soluble proteins of transplastomic N. oculata was assessed by measuring secretion of the cytokines IL-6, IL-10, and alpha-tumor necrosis (TNF-α), and cytotoxicity was assessed. These results provide a potential strategy to produce the Flo peptide in microalgae with antibacterial activities.

Properties
Additional details for this publication include:
Property NameValue
Journal CountryGermany
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
Copyright© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
Elocation10.1007/s11274-023-03664-7
DOI10.1007/s11274-023-03664-7
Journal AbbreviationWorld J Microbiol Biotechnol
Publication Date2023 May 30
eISSN1573-0972
ISSN1573-0972
Publication ModelElectronic
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PMID: PubMedPMID:37249711