Glucose-6-Phosphate Dehydrogenase from the Oleaginous Microalga Nannochloropsis Uncovers Its Potential Role in Promoting Lipogenesis

Overview
TitleGlucose-6-Phosphate Dehydrogenase from the Oleaginous Microalga Nannochloropsis Uncovers Its Potential Role in Promoting Lipogenesis
AuthorsXue J, Chen TT, Zheng JW, Balamurugan S, Liu YH, Yang WD, Liu JS, Li HY
TypeJournal Article
Journal NameBiotechnology journal
Volume15
Issue2
Year2020
Page(s)e1900135
CitationXue J, Chen TT, Zheng JW, Balamurugan S, Liu YH, Yang WD, Liu JS, Li HY. Glucose-6-Phosphate Dehydrogenase from the Oleaginous Microalga Nannochloropsis Uncovers Its Potential Role in Promoting Lipogenesis. Biotechnology journal. 2020 Feb; 15(2):e1900135.

Abstract

Microalgae have long been considered as potential biological feedstock for the production of wide array of bioproducts, such as biofuel feedstock because of their lipid accumulating capability. However, lipid productivity of microalgae is still far below commercial viability. Here, a glucose-6-phosphate dehydrogenase from the oleaginous microalga Nannochloropsis oceanica is identified and heterologously expressed in the green microalga Chlorella pyrenoidosa to characterize its function in the pentose phosphate pathway. It is found that the G6PD enzyme activity toward NADPH production is increased by 2.19-fold in engineered microalgal strains. Lipidomic analysis reveals up to 3.09-fold increase of neutral lipid content in the engineered strains, and lipid yield is gradually increased throughout the cultivation phase and saturated at the stationary phase. Moreover, cellular physiological characteristics including photosynthesis and growth rate are not impaired. Collectively, these results reveal the pivotal role of glucose-6-phosphate dehydrogenase from N. oceanica in NADPH supply, demonstrating that provision of reducing power is crucial for microalgal lipogenesis and can be a potential target for metabolic engineering.

Properties
Additional details for this publication include:
Property NameValue
Journal CountryGermany
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
Copyright© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Elocation10.1002/biot.201900135
DOI10.1002/biot.201900135
Journal AbbreviationBiotechnol J
Publication Date2020 Feb
eISSN1860-7314
ISSN1860-7314
Publication ModelPrint-Electronic
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PMID: PubMedPMID:31464064